CN102396461A - Use of mepiquat chloride plant growth regulator in increasing content of flavone active components in medicinal plants - Google Patents
Use of mepiquat chloride plant growth regulator in increasing content of flavone active components in medicinal plants Download PDFInfo
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- CN102396461A CN102396461A CN2011104260521A CN201110426052A CN102396461A CN 102396461 A CN102396461 A CN 102396461A CN 2011104260521 A CN2011104260521 A CN 2011104260521A CN 201110426052 A CN201110426052 A CN 201110426052A CN 102396461 A CN102396461 A CN 102396461A
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Abstract
By spraying mepiquat chloride plant growth regulator onto leaves, the content of flavone active components in roots of medicinal plants can be increased. The mepiquat chloride treatment is performed in a leaf expansion period. The method is suitable for root medicinal materials containing flavones compounds, such as scutellaria baicalensis.
Description
Technical field
The present invention relates to the traditional Chinese medicine cultivation, belongs to the Chinese medicine agriculture field.
Background technology
Active component content is an important indicator of weighing the traditional Chinese medicine quality, receives influence of various factors in the course of cultivation, comprises developmental stage, environment and cultivation condition etc.These factors must be optimized to realize the raising of cultivation medicinal material active component content.
Plant growth regulator can influence growth and development of plant, and influences the natural rhythm and pace of moving things of plant.More specifically, plant growth regulator can reduce plant height, promote seed sprouting, induced flowering, minimizing damage by disease and insect etc., but not see at present about utilizing plant growth regulator in actual production, to control the report of traditional Chinese medicine active component content.
Mepiquat chloride (Mepiquat chloride) is a kind of new plant growth regulator, is the antagonist of gibberellin, can be used for various crop; The performance multiple efficacies; Can promote development of plants, bloom, prevent to come off, increase output in advance, it is synthetic to strengthen chlorophyll, suppresses the elongation of stem and fruit branch.Mepiquat chloride has retarding action to vegetation growth of plant, and mepiquat chloride can absorb through plant leaf and root, conducts to complete stool, can reduce the activity of plant body inner gibberellin; Thereby the inhibition cell elongation, the terminal bud growing way weakens, and the control plant grows in length and breadth, and the plant internode is shortened; Plant type is compact, and the leaf color depth is thick, and leaf area reduces; And strengthen chlorophyllous synthesizing, can prevent the prosperous length of plant, postpone Feng Hang etc.Mepiquat chloride can improve cell membrane stability, increases plant resistance (Dong Zhixin, the mepiquat chloride application on crops, modernization agricultural, 1990,8:17.)。Mepiquat chloride uses wider on cotton, can prevent the cotton overgrowing effectively, and the control plant type is compact, reduces the shedding of cotton bolls, and promotion is ripe, makes the cotton raising the output.Can promote root system development; Leaf look greening; Thickening prevents excessive growth; Resistant to lodging; Improve into the bell rate; Spend before increasing frost; And cotton grade is improved; Simultaneously, make plant type compact; Superfluous bud significantly reduces, save the training recruitment (Luo Honghai, Zhao Ruihai, Han Chunli, Zhang Wangfeng, mepiquat chloride (DPC) be to the influence of cotton canopy structure characteristic and yield traits under the different densities, the cotton journal, 2011,4:334-340).In addition, mepiquat chloride be used for winter wheat can prevent lodging (Li Jiuxing, paclobutrazol and mepiquat chloride be to the research of wheat form and physiological effect, Agricultural University Of He'nan's journal, 1994,2:166-172); Be used for fruit tree and can suppress plant excessive growth, make plant solid, resistant to lodging with improve color and luster (Wang Menghui, Gao Yanrong, Li Hui, Chen Qingfu, the use of mepiquat chloride in Kuerle delicious pear tree summer pruning, northwest gardening, 2006,2:35); Be used for tomato and can improve output, and premature ripening (Liang Yuqin, Liu Yun, Liu Wen, Song Ping Yan, mepiquat chloride be to the influence of cherry and tomato plant strain growth output and quality, the Hebei agricultural science, and 2008,10:23-24).But also do not see the report that on medicinal plant, uses at present.
Flavonoids is one type of common traditional Chinese medicine active component, has anti-inflammatory, anti-oxidant, antitumor isoreactivity.The active component content raising that is realized by the present invention can be used for the traditional Chinese medicine that those contain the flavonoids active component, like the root of large-flowered skullcap (containing scutelloside, baicalein, wogonin etc.), the root of kudzu vine (containing Puerarin, big legumin etc.), Radix Glycyrrhizae (containing liquiritin etc.).The variation of flavonoid content and plant endogenous hormones level have close getting in touch, and show that like research the variation of root of large-flowered skullcap endogenous gibberellins content and scutelloside, baicalein content exist significant correlation property (Chen Shunqin, Huang Luqi; Yuan Yuan, Luo Yujian, Chen Ping; Qin Shuanshuan, Yang Zhaochun, illumination is to the influence of root of large-flowered skullcap endogenous hormones and active ingredient correlation; Chinese experimental pharmacology of traditional Chinese medical formulae magazine, 2010,16 (4): 87-90; Qin Shuanshuan, Huang Luqi, Yuan Yuan, water stress be to the influence of root of large-flowered skullcap endogenous hormones and active ingredient correlation, Chinese experimental pharmacology of traditional Chinese medical formulae magazine, 2010,16 (7): 75-77).
The growth and development stage of medicinal plant also can impact the accumulation of its active component content, shows that like research leaf stretching period is critical period (Yuan Y., the Hao J.D of active component accumulation in the root of large-flowered skullcap; Yang B., Li H., Li Z.; Huang L.Q., Feng X.F., Chen S.Q.; Luo Y.J.; And Zhang Z.J. (2010), Climate change affected the best producing area of Chinese herbal medicine Scutellaria baicalensis Georgi.J.Trad.Med. (Russia) .3s, 241-248.).Therefore, the present invention selects leaf stretching period to spray the mepiquat chloride plant growth regulator, to the root of large-flowered skullcap grow, and improve main active content of baicalin in the root of large-flowered skullcap.
Summary of the invention
The present invention relates to through selecting the plant growth regulator of suitable developmental stage, with the method that realizes that the activity of drug ingredients composition improves medicinal plant blade spraying effective dose.
The definition of embodiment 1 root of large-flowered skullcap growth and development stage
According to bibliographical information (Leeization, Huang Luqi, Yang Bin, Feng Xuefeng, Li Wei, soup, the Changing Pattern of the different growth and development stage active ingredients of the root of large-flowered skullcap, Chinese herbal medicine, 2008,4:604-607) confirm that the root of large-flowered skullcap growth and development stage that relates among the present invention is respectively:
1. the seedling phase is 8-10 to leaf period;
2. leaf stretching period is leaf and period of not blooming more than 10 pairs;
3. flowers and fruits are the period of blooming and not seeing fruit in earlier stage;
4. flowers and fruits are to bloom and period of result mid-term;
5. flowers and fruits are to finish at the florescence and period of result latter stage;
6. withering period is that seed maturity finishes and overground part withered and yellow period.
Embodiment 2 leaf stretching periods spray the influence of mepiquat chloride to root of large-flowered skullcap active component content.
Select annual root of large-flowered skullcap leaf stretching period, (Jiangnan Farm Chemical Plant, Changzhou produces, active ingredient >=97% to spray the mepiquat chloride plant growth regulator; Standard No.: GB9555-88), concentration is 50-200mg/L, sprays 1 time in every separated 8-10 days; Spraying number of times is 2-3 time, at leaf stretching period, flowers and fruits early stage and withering period the active component content of the root of large-flowered skullcap is measured respectively, and sample is by the five-spot collection; Each puts 3 strains, each 15 strains.Bright article are dried to constant weight for 55 ℃, pulverize, and put in the drier subsequent use.
Content assaying method adopts the RP-HPLC method to measure.Scutelloside (lot number 110715-200212, supply quantitative assay with), baicalein (lot number 111595-200301, supply quantitative assay with), wogonin (lot number 1514-200001, supply to differentiate with) standard items provide by Nat'l Pharmaceutical & Biological Products Control Institute.
Chromatographic condition: Diamonsil C18 (250mm * 4.6mm; 5 μ m); With acetonitrile-water-formic acid (21: 78: 1) (A) and acetonitrile-water-formic acid (80: 20: 1) be that flowing phase is carried out gradient elution: 0-10min (100%A) (B), 10-35min (100%A-90%A), 35-70min (90%A-65%A); 70-90min (65%A-5%A), 90-100min (5%A-0%A); Detect wavelength 280nm; 30 ℃ of column temperatures; Volume flow 1.0mL/min.The theoretical cam curve of scutelloside, baicalein, wogonin is respectively 20000,30000 and 200000.
Calibration curve is drawn: it is an amount of that precision takes by weighing scutelloside, baicalein, wogonin standard items, adds dissolve with methanol, makes mass concentration and be 0.561,0.066, the hybrid standard article solution of 0.015mg/mL.Get this hybrid standard article solution 0.5,1,6,12,18,24,30 μ L sample introduction respectively, with peak area sample size is returned, the regression equation that gets scutelloside, baicalein, wogonin respectively is Y=3.269 * 10
7X-2.773 * 10
6, r=0.9999; Y=5.269 * 10
7X-1.485 * 10
6, r=0.9997; Y=5.759 * 10
6X-2.492 * 10
4, r=0.9999.
Assay: get the about 0.1g of scutellariae,radix powder, the accurate title, decide, and puts in the tool plug conical flask, and precision pipettes 70% ethanol 20mL; It is fixed to claim, ultrasonic 1h is put coldly, claims quality; Supply the loss quality with 70% ethanol, filter, get subsequent filtrate 0.45 μ m miillpore filter and filter, promptly get.Measure according to above-mentioned chromatographic condition, adopt the external standard method one point method to calculate the content of scutelloside, baicalein and wogonin in each sample, the result is as shown in table 1, and the result shows that spraying mepiquat chloride can effectively strengthen content of baicalin in the withered and yellow phase scutellariae,radix.
Table 1 mepiquat chloride is to the influence of root of large-flowered skullcap active component content
Claims (4)
1. a method that improves medicinal plant flavonoids active component is characterized in that: utilize the mepiquat chloride plant growth regulator to improve medicinal plant flavonoids active component.
2. according to the method for the raising medicinal plant flavonoids active component of claim 1, wherein said medicinal plant is the root of large-flowered skullcap.
3. according to the method for the raising medicinal plant flavonoids active component of claim 1, wherein the usage amount of mepiquat chloride is 50-200mg/L.
4. according to the method for the raising medicinal plant flavonoids active component of claim 1, wherein the time of spraying is leaf stretching period.
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| CN110870476A (en) * | 2018-08-30 | 2020-03-10 | 沈阳中化农药化工研发有限公司 | Liquid preparation containing biphenyl compounds and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101810176A (en) * | 2009-12-08 | 2010-08-25 | 安阳市全丰农药化工有限责任公司 | Plant growth regulator containing cycocel and mepiquat chloride mixture |
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| CN101810176A (en) * | 2009-12-08 | 2010-08-25 | 安阳市全丰农药化工有限责任公司 | Plant growth regulator containing cycocel and mepiquat chloride mixture |
Non-Patent Citations (2)
| Title |
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| 李化等: "黄芩不同生长发育期有效成分的变化规律", 《中草药》, vol. 39, no. 04, 12 April 2008 (2008-04-12), pages 604 - 607 * |
| 蔡葛平等: "矮壮素和赤霉素对黄芩生物量及根中黄酮类成分产量的影响", 《中国农学通报》, vol. 24, no. 07, 5 July 2008 (2008-07-05), pages 213 - 217 * |
Cited By (1)
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| CN110870476A (en) * | 2018-08-30 | 2020-03-10 | 沈阳中化农药化工研发有限公司 | Liquid preparation containing biphenyl compounds and application thereof |
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