CN102391952A - In vitro conservation method of puccinia cnici - Google Patents
In vitro conservation method of puccinia cnici Download PDFInfo
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- CN102391952A CN102391952A CN2011103691582A CN201110369158A CN102391952A CN 102391952 A CN102391952 A CN 102391952A CN 2011103691582 A CN2011103691582 A CN 2011103691582A CN 201110369158 A CN201110369158 A CN 201110369158A CN 102391952 A CN102391952 A CN 102391952A
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Abstract
The invention discloses an in vitro conservation method of puccinia cnici, which is characterized in that the in vitro conservation method is operated according to the following steps: a) preparing uredispore powder; b) taking one type of solid particles, spraying 1% carboxymethyl cellulose sodium water solution on the surfaces of the solid particles and then rolling a layer of the uredispore powder; c) using A powder for coating 10-15 layers so as to form particles taking the solid particles as cores; d) using B powder for coating a second outer layer; e) using talcum powder for coating an outermost layer, and f) airing and storing. The component of the A powder is goldleaf brand tobacco coating agent type A which is produced by Liuhe Institute of Agricultural Science. The component of the B component is goldleaf brand tobacco coating agent type B which is produced by Liuhe Institute of Agricultural Science. The method is simple, convenient to operate and easy for conservation; furthermore, under the in vitro conservation conditions, the germination ratio of uredispore is high, the conservation effect is good and the purpose of developing biocontrol preparations can be achieved.
Description
Technical field:
The present invention relates to agricultural biological technical field, the in-vitro conservation method of particularly a kind of Ji handle rest fungus.
Background technology:
Rest fungus, mycelia has barrier film, the primary hyphae monokaryon, double-coreization subsequently is grown in host's intercellular substance, invades cell with haustorium.Haustorium generally closely combines round nucleus and tenuigenin.Contain karyomicrosome, plastosome, meat net, glycogen particle and mixtinite in the tenuigenin of rest fungus.The membranous structure of mycelia is different with high basidiomycetes, and approximate with ascomycetes, entad growth respectively has 1 layer of electronics dense layer up and down, and the centre is 1 layer of thin layer of electronics, from around to center attenuation gradually.There are the hole that is filled the thing filling, uncovered shape structure up and down in central authorities.Rest fungus does not give birth to sporophore, only gives birth to spore device and spore.Most typical rest fungus has 5 types of spores, i.e. spermatium, spring spore, uredospore, winter spore and sporidium, and wherein uredospore can produce repeatedly in the season of growth continuously, is the main spore shape of most plant rusts, in the generation of plant rust, plays a leading role.Rest fungus is prone to cause plant rust takes place, and rest fungus can only grow on the host plant body of living under field conditions (factors), after host plant is infected by aecidium, grows the sorus of iron rust shape at plant organ tissues such as blade, leaf sheath or stems.Rust is popular very strong Plant diseases.
We had found to dye the weeds field thistle plant of rest fungus in the area, Qingdao in 2006, and ill plant the lighter downgrades, or can not normally blossom and have seeds, and weight person is dead.Sick leaf back overgrows with the spore powder of rust.The applicant identifies the rest fungus of field thistle plant, finds that it belongs to Basidiomycotina, Teliomycetes, Uredinales, handle rest fungus section, Puccinia, Ji handle rest fungus (Puccinia obtegens).Uredospore subcircular, tawny are to brown, and diameter is 26-27.5 μ m, and fine spinule is arranged.Winter spore is short oval, and two cell sizes are 32-39 * 21-27 μ m, and brown has fine little wart, the tabula place institute of not hanging, and handle is very short, water white transparency.The applicant has carried out research at length to this rest fungus, in the hope of opening up new resource for the biological control of weeds field thistle.And, with the new bacterium source of this rest fungus as the biological control of weeds field thistle.
For a long time, weed control problem has proposed stern challenge to agriculture prodn.Statisticses such as McWhorter etc. and Parker show that the global farm crop mean yield loss that is caused by weeds is approximately 12%.The 1950's, the sales volume of weedicide only accounted for 20% of whole agrochemical agent Total sales volume, and rise to 50% the eighties in 20th century, after this rises to 60%.The extensive application of chemical herbicide obviously exposes many drawbacks, at first is the weedicide float of mist droplet, the soup volatilization, and selectivity is undesirable, the degraded product damage to crops, even cause chemical injury of crops; Next is to use weedicide to cause rising, the appearance of antiweed weeds and the increasing the weight of of environmental pollution of resistance weeds population repeatedly.This shows that seeking safer effective weedicide has become weedicide industry developing direction from now on.Campelyco helps environment protection, and effective to weed control.Especially phytopathy original weedicide is more and more appeared out advantage suddenly, excites people that it is produced keen interest to a certain extent.Field thistle is claimed field thistle [Cephalanoplos segetum (Bunge) Kitam.] again, is one of the main weeds in China farmland (comprising vega), takes place very extensively.Field thistle is the same with other farmland weed to compete water, fertilizer and space with crop, directly influences output and the quality of crop.At present farmland weed prevent and kill off main dependence artificial weeding with chemical herbicide is prevented and kill off, the use of chemical pesticides also is the major obstacle of nuisanceless leaf tobacco production in a large number., study, in the hope of opening up new approach for the biological control of weeds such as field thistle to field thistle cause of disease rest fungus for this reason.
Usually rest fungus can only preserve on the live body plant, that is, rest fungus can not be cultivated on artificial medium.Therefore, it is simple, easy to operate to press for a kind of preservation of development, and the uredospore germination rate is high, and can reach development biological prevention and control agent purpose in-vitro conservation method.
Summary of the invention:
The objective of the invention is to overcome the shortcoming that exists in the prior art, it is simple, easy to operate to invent a kind of new preservation, and the uredospore germination rate is high, and can reach development biological prevention and control agent purpose Ji handle rest fungus in-vitro conservation method.
To achieve these goals, the invention provides a kind of Ji handle rest fungus in-vitro conservation method, wherein, according to following procedure:
A, preparation uredospore powder,
B, get a kind of solid particulate, and, roll last layer uredospore powder then at its surface sprinkling one deck 1% sodium carboxymethyl cellulose solution,
10～15 layers of c, A powder dressings, forming with the solid particulate is the particle of core,
D, B powder dressing time skin,
E, talcum powder dressing outermost layer,
F, dry and preserve.
Step a is: the field thistle plant of Ji handle rest fungus has been infected in artificial collection from the field, and then at the indoor monospore purifying that carries out, the artificial inoculation field thistle makes field thistle infect Ji handle rest fungus.Scrape and get sophisticated uredospore from the little thistle leaves of morbidity with scalpel then, be mixed and made into the uredospore powder scraping the uredospore of getting and the A powder of its 3～5 times of weight fractions.
Step b is: weigh the solid particulate of getting with weight such as uredospore powder in the balance, spraying the massfraction ratio at particle surface then is 1% sodium carboxymethyl cellulose solution, fully stirs to make sodium carboxymethyl cellulose solution be evenly distributed in particle surface;
Particle is mixed with the uredospore powder, and the rolling particle, all evenly roll until all uredospore powder and be bonded at the particulate surface.
Step c is: spraying the massfraction ratio at particle surface once more is 1% sodium carboxymethyl cellulose solution, and the spray back is rolled at particle surface again and is stained with 1 layer in A powder;
Aforesaid operations repeats 10～15 times, until the particle that is stained with the A powder, can be as the criterion through 8 orders～10 purpose sieve apertures, and the particle requirement size that is stained with the A powder is even.
Steps d is: the particulate surface sprinkling massfraction ratio at step c gained is 1% sodium carboxymethyl cellulose solution, evenly rolls and is stained with 1 layer in B powder.
Step e is: the particulate surface sprinkling massfraction ratio at the steps d gained is 1% sodium carboxymethyl cellulose solution, rolls uniformly and is stained with talcum powder, makes particle surface form the talcum bisque.
Step f is: sieve, choose and passed through 8 orders～10 purpose particles, and under indoor room temperature, dried 20～28 hours; The air dried particle is packed in the centrifuge tube and sealed centrifuge tube, preserve with-20 ℃ of refrigerators in 4 ℃.
The composition of said A powder is: gold leaf board tobacco seed-coating agent model A, it is produced with research of agricultural science by six.
The composition of said B powder is: gold leaf board tobacco seed-coating agent model B, it is produced with research of agricultural science by six.
When Ji handle rest fungus uredospore preserved 120 days through store method of the present invention, its germination rate was higher than 80%.
The size of said solid particulate is 40～50 orders, and said solid particulate is any in tobacco seed, Semen Brassicae campestris, Chinese cabbage seeds or the silica gel particle of deactivation.
The concrete operations of repetitive operation 10～15 times are among the step c: " spraying the massfraction ratio in solid particles surface is 1% sodium carboxymethyl cellulose solution, and the spray back is rolled in solid particles surface again and is stained with 1 layer in A powder.
The invention has the advantages that method is simple, easy to operate, be easy to preserve, preservation effect is good, and the uredospore germination rate is high, can reach the in vitro conservation of development biological prevention and control agent purpose.Be specially:
Usually the pathogenic rest fungus can only preserve on the live body plant, that is, rest fungus can not be cultivated on artificial medium, and the present invention adopts the in vitro conservation mode, has innovated store method, can guarantee again to have high germination rate through the rest fungus of this preservation.
Utilize the A powder and the B powder of tobacco seed-coating agent that uredospore is carried out dressing, 10～15 layers of A powder dressing uredospores, B powder dressing time skin, talcum powder dressing outermost layer,
This store method is simple, rest fungus is become be easy to preserve, and preservation effect is good.
Store method of the present invention, the research of support Ji handle rest fungus that can be strong is in the hope of opening up new approach for the biological control of weeds such as field thistle.
Description of drawings:
Fig. 1 is the block diagram of store method of the present invention.
Embodiment:
Below in conjunction with accompanying drawing, invention is described.
As shown in Figure 1, Fig. 1 is the block diagram of store method of the present invention.
The invention provides the in-vitro conservation method of a kind of Ji handle rest fungus, wherein, according to following procedure:
A, preparation uredospore powder,
B, get a kind of solid particulate, and, roll last layer uredospore powder then at its surface sprinkling one deck 1% sodium carboxymethyl cellulose solution,
10～15 layers of c, A powder dressings, forming with the solid particulate is the particle of core,
D, B powder dressing time skin,
E, talcum powder dressing outermost layer,
F, dry and preserve.
Step a is: the field thistle plant of Ji handle rest fungus has been infected in artificial collection from the field, and then at the indoor monospore purifying that carries out, the artificial inoculation field thistle makes field thistle infect Ji handle rest fungus.Scrape and get sophisticated uredospore from the little thistle leaves of morbidity with scalpel then, be mixed and made into the uredospore powder scraping the uredospore of getting and the A powder of its 3～5 times of weight fractions.
Step b is: weigh the solid particulate of getting with weight such as uredospore powder in the balance, spraying the massfraction ratio at particle surface then is 1% sodium carboxymethyl cellulose solution, fully stirs to make sodium carboxymethyl cellulose solution be evenly distributed in particle surface;
Particle is mixed with the uredospore powder, and the rolling particle, all evenly roll until all uredospore powder and be bonded at the particulate surface.
Step c is: spraying the massfraction ratio at particle surface once more is 1% sodium carboxymethyl cellulose solution, and the spray back is rolled at particle surface again and is stained with 1 layer in A powder; Aforesaid operations repeats 10～15 times, until the particle that is stained with the A powder, can be as the criterion through 8 orders～10 purpose sieve apertures, and the particle requirement size that is stained with the A powder is even.
Steps d is: the particulate surface sprinkling massfraction ratio at step c gained is 1% sodium carboxymethyl cellulose solution, evenly rolls and is stained with 1 layer in B powder.
Step e is: the particulate surface sprinkling massfraction ratio at the steps d gained is 1% sodium carboxymethyl cellulose solution, rolls uniformly and is stained with talcum powder, makes particle surface form the talcum bisque.
Step f is: sieve, choose and passed through 8 orders～10 purpose particles, and under indoor room temperature, dried 20～28 hours; The air dried particle is packed in the centrifuge tube and sealed centrifuge tube, preserve with-20 ℃ of refrigerators in 4 ℃.The composition of A powder is: gold leaf board tobacco seed-coating agent model A, it is produced with research of agricultural science by six.The composition of B powder is: gold leaf board tobacco seed-coating agent model B, it is produced with research of agricultural science by six.When Ji handle rest fungus uredospore preserved 120 days through store method of the present invention, its germination rate reached more than 80%.The size of solid particulate is 40～50 orders, and said solid particulate is any in tobacco seed, Semen Brassicae campestris, Chinese cabbage seeds or the silica gel particle of deactivation.
Example 1
At first the field thistle plant that infects Ji handle rest fungus is gathered in manual work from the field, and then at the indoor monospore purifying that carries out, the artificial inoculation field thistle makes field thistle infect Ji handle rest fungus.Scrape and get uredospore from the little thistle leaves of morbidity with scalpel then, be mixed and made into the uredospore powder scraping the uredospore of getting and the A powder of its 3～5 times of volumes; Take by weighing tobacco seeds weight, deactivation such as uredospore powder then, the tobacco seed size is selected 40～45 orders for use, and at tobacco seed surface sprinkling 1% sodium carboxymethyl cellulose solution; Roll the uredospore powder on the tobacco seed surface then; At tobacco seed surface sprinkling 1% sodium carboxymethyl cellulose solution, roll the A powder again; Repeat to spray 1% sodium carboxymethyl cellulose solution, and roll the A powder 10～15 times, can pass through 8 orders～10 purpose sieve apertures until particle diameter; Spray 1% sodium carboxymethyl cellulose solution, roll 1 layer in B powder again; Spray 1% sodium carboxymethyl cellulose solution, roll 1 layer of talcum powder; Sieve, and at the indoor 20～28h that dries; Particle is enclosed in the centrifuge tube, in 4 ℃ with-20 ℃ of conditions under preserve.The uredospore germination rate is higher than 80% when preserving 120 days.(sprouting result such as following table)
The uredospore that preserves sprouts the result
Example 2
A, preparation uredospore powder,
B, get Semen Brassicae campestris, and, roll last layer uredospore powder then at its surface sprinkling one deck 1% sodium carboxymethyl cellulose solution,
10～15 layers of c, A powder dressings, forming with the Semen Brassicae campestris is the particle of core,
D, B powder dressing time skin,
E, talcum powder dressing outermost layer,
F, dry and preserve.
Step a is: the field thistle plant of Ji handle rest fungus has been infected in artificial collection from the field, and then at the indoor monospore purifying that carries out, the artificial inoculation field thistle makes field thistle infect Ji handle rest fungus.Scrape and get sophisticated uredospore from the little thistle leaves of morbidity with scalpel then, be mixed and made into the uredospore powder scraping the uredospore of getting and the A powder of its 3～5 times of weight fractions.
Step b is: weigh the solid particulate of getting with weight such as uredospore powder in the balance; Solid particulate is the Semen Brassicae campestris of fire extinguishing; Size is 48～50 orders; Be 1% sodium carboxymethyl cellulose solution at Semen Brassicae campestris surface sprinkling massfraction ratio then, fully stirring makes sodium carboxymethyl cellulose solution be evenly distributed in the Semen Brassicae campestris surface; The Semen Brassicae campestris that is stained with sodium carboxymethyl cellulose solution is mixed with the uredospore powder, and the rolling Semen Brassicae campestris, the surface that is bonded at Semen Brassicae campestris all evenly rolled until all uredospore powder.
Step c is: be 1% sodium carboxymethyl cellulose solution at Semen Brassicae campestris surface sprinkling massfraction ratio once more, the spray back is rolled on the Semen Brassicae campestris surface again and is stained with 1 layer in A powder; Aforesaid operations repeats 10～15 times, and the particle that forms until the Semen Brassicae campestris that is stained with the A powder can be as the criterion through 8 orders～10 purpose sieve apertures, and the particle requirement size that is stained with the A powder is even.
Steps d is: the particulate surface sprinkling massfraction ratio at step c gained is 1% sodium carboxymethyl cellulose solution, evenly rolls and is stained with 1 layer in B powder.
Step e is: the particulate surface sprinkling massfraction ratio at the steps d gained is 1% sodium carboxymethyl cellulose solution, rolls uniformly and is stained with talcum powder, makes particle surface form the talcum bisque.
Step f is: sieve, choose and passed through 8 orders～10 purpose particles, and under indoor room temperature, dried 20～28 hours; The air dried particle is packed in the centrifuge tube and sealed centrifuge tube, preserve with-20 ℃ of refrigerators in 4 ℃.The composition of A powder is: gold leaf board tobacco seed-coating agent model A, it is produced with research of agricultural science by six.The composition of B powder is: gold leaf board tobacco seed-coating agent model B, it is produced with research of agricultural science by six.When Ji handle rest fungus uredospore preserved 120 days through store method of the present invention, its germination rate was higher than 80%.(sprouting result such as following table)
The uredospore that preserves sprouts the result
Example 3
A, preparation uredospore powder,
B, get Chinese cabbage seeds, and, roll last layer uredospore powder then at its surface sprinkling one deck 1% sodium carboxymethyl cellulose solution,
10～15 layers of c, A powder dressings, forming with the Chinese cabbage seeds is the particle of core,
D, B powder dressing time skin,
E, talcum powder dressing outermost layer,
F, dry and preserve.
Step a is: the field thistle plant of Ji handle rest fungus has been infected in artificial collection from the field, and then at the indoor monospore purifying that carries out, the artificial inoculation field thistle makes field thistle infect Ji handle rest fungus.Scrape and get sophisticated uredospore from the little thistle leaves of morbidity with scalpel then, be mixed and made into the uredospore powder scraping the uredospore of getting and the A powder of its 3～5 times of weight fractions.
Step b is: weigh the solid particulate of getting with weight such as uredospore powder in the balance; Solid particulate is the Chinese cabbage seeds of deactivation; Size is 48～50 orders; Be 1% sodium carboxymethyl cellulose solution at Chinese cabbage seeds surface sprinkling massfraction ratio then, fully stirring makes sodium carboxymethyl cellulose solution be evenly distributed in the Chinese cabbage seeds surface; The Chinese cabbage seeds that is stained with sodium carboxymethyl cellulose solution is mixed with the uredospore powder, and the rolling Chinese cabbage seeds, the surface that is bonded at Chinese cabbage seeds all evenly rolled until all uredospore powder.
Step c is: be 1% sodium carboxymethyl cellulose solution at Chinese cabbage seeds surface sprinkling massfraction ratio once more, the spray back is rolled at particle surface again and is stained with 1 layer in A powder; Aforesaid operations repeats 10～15 times, and the particle until the Chinese cabbage seeds that is stained with the A powder forms can be as the criterion through 8 orders～10 purpose sieve apertures, and the particle requirement size that is stained with the A powder is even.
Steps d is: at step c gained particulate surface sprinkling massfraction ratio is 1% sodium carboxymethyl cellulose solution, evenly rolls and is stained with 1 layer in B powder.
Step e is: the particulate surface sprinkling massfraction ratio at the steps d gained is 1% sodium carboxymethyl cellulose solution, rolls uniformly and is stained with talcum powder, makes particle surface form the talcum bisque.
Step f is: sieve, choose and passed through 8 orders～10 purpose particles, and under indoor room temperature, dried 20～28 hours; The air dried particle is packed in the centrifuge tube and sealed centrifuge tube, preserve with-20 ℃ of refrigerators in 4 ℃.The composition of A powder is: gold leaf board tobacco seed-coating agent model A, it is produced with research of agricultural science by six.The composition of B powder is: gold leaf board tobacco seed-coating agent model B, it is produced with research of agricultural science by six.When Ji handle rest fungus uredospore preserved 120 days through store method of the present invention, its germination rate was higher than 80%.(sprouting result such as following table)
The uredospore that preserves sprouts the result
Example 4
At first the field thistle plant that infects Ji handle rest fungus is gathered in manual work from the field, and then at the indoor monospore purifying that carries out, the artificial inoculation field thistle makes field thistle infect Ji handle rest fungus.Scrape and get uredospore from the little thistle leaves of morbidity with scalpel then, be mixed and made into the uredospore powder scraping the uredospore of getting and the A powder of its 3～5 times of volumes; Take by weighing the silica gel particle of weight such as uredospore powder then, the silica gel particle size is selected 40 orders for use, and sprays 1% sodium carboxymethyl cellulose solution at silica gel particle surface; Uredospore powder on silica gel particle surface rolls then; Spray 1% sodium carboxymethyl cellulose solution at silica gel particle surface again, roll the A powder; Repeat to spray 1% sodium carboxymethyl cellulose solution, and roll the A powder 10～15 times, can pass through 8 orders～10 purpose sieve apertures until the silica gel particle diameter; Spray 1% sodium carboxymethyl cellulose solution, roll 1 layer in B powder again; Spray 1% sodium carboxymethyl cellulose solution, roll 1 layer of talcum powder; Sieve, and at the indoor 20～28h that dries; Silica gel particle is enclosed in the centrifuge tube, in 4 ℃ with-20 ℃ of conditions under preserve.The uredospore germination rate is higher than 80% when preserving 120 days.(sprouting result such as following table)
The uredospore that preserves sprouts the result
Advantage method of the present invention is simple, easy to operate, is easy to preserve, and preservation effect is good, and the uredospore germination rate is high, can reach the in vitro conservation technology of development biological prevention and control agent purpose.
Usually rest fungus can only preserve on the live body plant, that is, rest fungus can not be cultivated on artificial medium, and the present invention adopts the in vitro conservation mode, has innovated store method, can guarantee again to have high germination rate through the rest fungus that this method is preserved.Utilize tobacco seed-coating agent A powder and B powder, 10～15 layers of A powder dressing uredospores, B powder dressing time skin, talcum powder dressing outermost layer.
This store method is simple, rest fungus is become be easy to preserve, and its preservation effect is good.Store method of the present invention, the research of support rest fungus that can be strong is in the hope of opening up new approach for the biological control of weeds such as field thistle.
Claims (10)
1. the in-vitro conservation method of a Ji handle rest fungus is characterized in that, according to following procedure:
A, preparation uredospore powder,
B, get a kind of solid particulate, and, roll last layer uredospore powder then at its surface sprinkling one deck 1% sodium carboxymethyl cellulose solution
10～15 layers of c, A powder dressings, forming with the solid particulate is the particle of core,
D, B powder dressing time skin,
E, talcum powder dressing outermost layer,
F, dry and preserve.
Step a is: the field thistle plant of Ji handle rest fungus has been infected in artificial collection from the field, and then at the indoor monospore purifying that carries out, the artificial inoculation field thistle makes field thistle infect Ji handle rest fungus.Scrape and get sophisticated uredospore from the little thistle leaves of morbidity with scalpel then, be mixed and made into the uredospore powder scraping the uredospore of getting and the A powder of its 3～5 times of weight fractions.
2. according to the store method of the said Ji handle of claim 1 rest fungus, it is characterized in that step b is:
Weigh the solid particulate of getting with weight such as uredospore powder in the balance, spraying the massfraction ratio at particle surface then is 1% sodium carboxymethyl cellulose solution, fully stirs to make sodium carboxymethyl cellulose solution be evenly distributed in particle surface; Particle is mixed with the uredospore powder, and the rolling particle, all evenly roll until all uredospore powder and be bonded at the particulate surface.
3. according to the store method of the said Ji handle of claim 2 rest fungus, it is characterized in that step c is:
Spraying the massfraction ratio at particle surface once more is 1% sodium carboxymethyl cellulose solution, and the spray back is rolled at particle surface again and is stained with 1 layer in A powder; Aforesaid operations repeats 10～15 times, until the particle that is stained with the A powder, can be as the criterion through 8 orders～10 purpose sieve apertures, and the particle requirement size that is stained with the A powder is even.
4. according to the store method of the said Ji handle of claim 3 rest fungus, it is characterized in that steps d is:
Spraying the massfraction ratio at step c gained particle surface is 1% sodium carboxymethyl cellulose solution, evenly rolls and is stained with 1 layer in B powder.
5. according to the store method of the said Ji handle of claim 4 rest fungus; It is characterized in that; Step e is: spraying the massfraction ratio at steps d gained particle surface is 1% sodium carboxymethyl cellulose solution, rolls uniformly and is stained with talcum powder, makes particle surface form the talcum bisque.
6. according to the store method of the said Ji handle of claim 5 rest fungus, it is characterized in that step f is: sieve, choose and passed through 8 orders～10 purpose particles, and under indoor room temperature, dried 20～28 hours; The air dried particle is packed in the centrifuge tube and sealed centrifuge tube, preserve with-20 ℃ of refrigerators in 4 ℃.
7. according to the store method of the said Ji handle of claim 1 rest fungus, it is characterized in that the composition of said A powder is: gold leaf board tobacco seed-coating agent model A, it is produced with research of agricultural science by six.
8. according to the store method of the said Ji handle of claim 1 rest fungus, it is characterized in that the composition of said B powder is: gold leaf board tobacco seed-coating agent model B, it is produced with research of agricultural science by six.
9. according to the store method of the said Ji handle of claim 1 rest fungus, it is characterized in that when Ji handle rest fungus uredospore preserved 120 days through store method of the present invention, its germination rate was higher than 80%.
10. according to the store method of the said Ji handle of claim 2 rest fungus, it is characterized in that the size of said solid particulate is 40～50 orders, said solid particulate is any in tobacco seed, Semen Brassicae campestris, Chinese cabbage seeds or the silica gel particle of deactivation.
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| CN108486033A (en) * | 2018-02-23 | 2018-09-04 | 云南省德宏热带农业科学研究所 | A kind of in-vitro verification method of coffee germplasm rust resistance |
| CN117004497A (en) * | 2023-08-14 | 2023-11-07 | 中国农业科学院植物保护研究所 | Method for preserving wheat stripe rust fungus summer spores |
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| 李金玉 等: "种衣剂17号包衣对小麦条锈菌影响的组织病理学研究", 《植物保护学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108486033A (en) * | 2018-02-23 | 2018-09-04 | 云南省德宏热带农业科学研究所 | A kind of in-vitro verification method of coffee germplasm rust resistance |
| CN117004497A (en) * | 2023-08-14 | 2023-11-07 | 中国农业科学院植物保护研究所 | Method for preserving wheat stripe rust fungus summer spores |
| CN117004497B (en) * | 2023-08-14 | 2024-04-19 | 中国农业科学院植物保护研究所 | Method for preserving wheat stripe rust fungus summer spores |
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