CN102391668A - Preparation method of purple sweet potato haematochrome with high color value - Google Patents
Preparation method of purple sweet potato haematochrome with high color value Download PDFInfo
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- CN102391668A CN102391668A CN2011102852233A CN201110285223A CN102391668A CN 102391668 A CN102391668 A CN 102391668A CN 2011102852233 A CN2011102852233 A CN 2011102852233A CN 201110285223 A CN201110285223 A CN 201110285223A CN 102391668 A CN102391668 A CN 102391668A
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Abstract
The invention discloses a preparation method of purple sweet potato haematochrome with high color value. The preparation method includes the steps as follows: firstly grinding a purple sweet potato into pulp; adding the pulp in a citric acid aqueous solution; conducting ultrasonic extraction in normal temperature; conducting centrifugation to obtain crude extract solution; secondly, allowing the extract solution to pass through a chromatographic column; absorption and elution, conducting refining, thus obtaining the purple sweet potato haematochrome; and conducting concentration and drying, thus obtaining purple sweet potato haematochrome powder.
Description
Technical field
The invention belongs to a kind of extraction and purification process of functional natural pigment; The preparation method who relates to a kind of high luminance relay valency purple sweet potato haematochrome adopts aqueous citric acid solution supersound extraction, extracting solution purifying, the isolating method of chromatography column to prepare high luminance relay valency purple sweet potato haematochrome.
Background technology
Food-processing industry needs a large amount of food dyes, promotes competitiveness of product, is the important Oranoleptic indicator of food, but the majority that foodstuffs industry is used at present is artificial synthetic chemical pigment, and is most harmful.In recent years, find successively that both at home and abroad the toxic action of chemosynthesis pigment not only shows as general toxicity and causes rushing down property, some kind also has carcinogenesis.So usually replace synthetic colour with self colour now, security improves greatly, some is also to the human body beneficial.
China's anthocyanin class plant resources is very abundant; Wherein many people of being get used to stem, leaf, flower and the piece root of edible and medicinal berry, drupelet or plant; Be no lack of the edible natural pigment of value of exploiting and utilizing glycosides is arranged; Key is to filter out bright-colored, good stability, strong coloring force, raw material to contain abundant and the easy product of complete processing, and this product has the bigger market competitiveness.
In recent years, domestic development and use to the edible natural pigment resource obtain bigger progress, aspect anthocyani pigment, have also carried out many researchs.The primary structure of sweet potato anthocyanogen is cyanidin acyl group glucoside and YGM 6 acyl group glucosyl, and the acidylate group that it had makes it, and generally anthocyanogen is less to inductions such as light, heat, air, and character is more stable.In addition, the sweet potato natural pigment is because raw material sources are easy, cheap; Extraction cost is low, can be widely used as the pigment source of food colorant, and its sub product can fully utilize still; Process raw material as food, chemical industry, feed etc.; This drives sweet potato propagation to improving the comprehensive utilization ratio of sweet potato, has very important significance.
Purple sweet potato haematochrome is the natural food colour that from the convolvulaceae morning glory, extracts, and by cyanidin(e), flavonoid is formed, and the vivid nature of color and luster is nontoxic, no special odor.It has multiple nutrients, pharmacology and nourishing function; It is a kind of rare natural food colour resource; Be widely used in the food service industry, simultaneously, because purple sweet potato haematochrome has stronger anti-oxidant, removing radical ability; Has mutation property, antioxygenation and physiological function such as antitumor.According to the data introduction, cyanidin(e) resistance of oxidation in animal body is 20 times of VE, 50 times of VC, thereby food, makeup and medical aspect, cyanidin(e) has huge application potential.The purple sweet potato haematochrome good heat resistance; Can stand the Pasteur's method sterilization in the foodstuffs industry; Can be dissolved in alcohol and water solution; Therefore mainly add in the food such as ice-creams, dairy beverage, cheese, fishery products, nectar, jelly, cereal, belong to the Nantural non-toxic goods as redness to the red-purple tinting material of food.
At present acidifying ethanol or acidifying methyl alcohol are adopted in the extraction of purple sweet potato haematochrome more, this causes dissolvent residual to a certain extent, and extraction yield neither be very high.Occurred the method with the aqueous citric acid solution extraction in recent years, but its purification process is improper, can't obtain high luminance relay valency purple sweet potato haematochrome, and proper for some purification process, its extraction yield is undesirable again.
Summary of the invention
To the deficiency of purple sweet potato haematochrome specification and prior art on the market, technical problem to be solved by this invention is: the extraction yield of raw material is low, product look valency is low, complex process, defective that production cost is high.A kind of preparation method who comes the separation and purification purple sweet potato haematochrome with the method for aqueous acid ultrasonic extraction, extracting solution purifying, column chromatography is provided.
In order to solve the problems of the technologies described above, the technical scheme that the present invention adopts is, a kind of method that improves purple sweet potato haematochrome look valency, and it comprises following content:
At first purple sweet potato powder is broken into slurry, is placed on again in the aqueous citric acid solution, normal temperature supersound extraction, the centrifugal crude extract solution that obtains; Secondly extracting solution is crossed chromatography column,, make the purple sweet potato haematochrome powder through concentrated, drying again through adsorption process, the refining liquid purple sweet potato haematochrome that obtains of elution process.Concrete extraction and separation method is following:
1, Rhizoma Dioscoreae esculentae being added 5 ~ 20 times of mass concentrations is to smash pulping to pieces in 0.2 ~ 0.8% the aqueous citric acid solution;
2, with above-mentioned slurries supersound extraction 10 ~ 120min under the frequency of 20 ~ 150KHz, centrifugal, collect extracting solution;
3, said extracted liquid is crossed AB-8 or 3520 resin columns, 40 ~ 95% ethanol elutions;
4, above-mentioned elutriant is descended purple sweet potato haematochrome powder concentrated, that drying obtains the high luminance relay valency in 40 ~ 60 ℃.
Description of drawings
Fig. 1 is the liquid phase collection of illustrative plates of purple sweet potato haematochrome.
Specific embodiments
The present invention can specify its preparation process and effect according to the following example.
Embodiment 1:
1, Rhizoma Dioscoreae esculentae being added 10 times of mass concentrations is to smash pulping to pieces in 0.6% the aqueous citric acid solution, and supersound extraction 10min is centrifugal under the frequency of 150KHz; It is to smash pulping to pieces in 0.3% the aqueous citric acid solution that the filter residue of centrifugal gained adds 2 times of mass concentrations again; Supersound extraction 40min is centrifugal under the frequency of 60KHz, and extracted twice liquid is merged; Collect extracting solution, the extraction yield that records in the extracting solution is 97.5%;
2, said extracted liquid is crossed the AB-8 resin column, 60% ethanol elution;
3, above-mentioned elutriant is concentrated under 60 ℃, drying obtains the purple sweet potato haematochrome powder, and the look valency is 78.
Embodiment 2:
1, Rhizoma Dioscoreae esculentae being added 5 times of mass concentrations is to smash pulping to pieces in 0.5% the aqueous citric acid solution; Supersound extraction 20min is centrifugal under the frequency of 60KHz, and the filter residue of centrifugal gained extracts for the second time according to above-mentioned said method again; Centrifugal; Extracted twice liquid is merged, collect extracting solution, the extraction yield that records in the extracting solution is 99.0%;
2, said extracted liquid is crossed the AB-8 resin column, 80% ethanol elution;
3, above-mentioned elutriant is concentrated under 60 ℃, drying obtains the purple sweet potato haematochrome powder, and the look valency is 103.
Embodiment 3:
1, Rhizoma Dioscoreae esculentae being added 5 times of mass concentrations is to smash pulping to pieces in 0.5% the aqueous citric acid solution; Extract 2h at normal temperatures, centrifugal, the filter residue of centrifugal gained extracts for the second time according to above-mentioned said method again; Centrifugal; Extracted twice liquid is merged, collect extracting solution, the extraction yield that records in the extracting solution is 86%;
2, said extracted liquid is crossed the AB-8 resin column, 80% ethanol elution;
3, above-mentioned elutriant is concentrated under 60 ℃, drying obtains the purple sweet potato haematochrome powder, and the look valency is 101.
Embodiment 4:
1, Rhizoma Dioscoreae esculentae being added 8 times of mass concentrations is to smash pulping to pieces in 0.2% the aqueous citric acid solution, and supersound extraction 120min is centrifugal under the frequency of 60KHz, and the extraction yield that records in the extracting solution is 95.5%;
2, said extracted liquid is crossed the AB-8 resin column, 45% ethanol elution;
3, above-mentioned elutriant is concentrated under 60 ℃, drying obtains the purple sweet potato haematochrome powder, and the look valency is 67.
Embodiment 5:
1, Rhizoma Dioscoreae esculentae being added 20 times of mass concentrations is to smash pulping to pieces in 0.4% the aqueous citric acid solution, and supersound extraction 30min is centrifugal under the frequency of 20KHz, and the extraction yield that records in the extracting solution is 96.5%;
2, said extracted liquid is crossed 3520 resin columns, 80% ethanol elution;
3, above-mentioned elutriant is concentrated under 60 ℃, drying obtains the purple sweet potato haematochrome powder, and the look valency is 93.
Claims (9)
1. the preparation method of a high luminance relay valency purple sweet potato haematochrome is characterized in that: purple sweet potato powder is broken into slurry, is placed in the aqueous citric acid solution again, normal temperature supersound extraction, the centrifugal crude extract solution that obtains; Secondly extracting solution is crossed chromatography column,, make the purple sweet potato haematochrome powder through concentrated, drying again through adsorption process, the refining liquid purple sweet potato haematochrome that obtains of elution process.
2. the preparation method of high luminance relay valency purple sweet potato haematochrome according to claim 1 is characterized in that: the mass concentration of aqueous citric acid solution is 0.2 ~ 0.8%, and the solid-to-liquid ratio of Rhizoma Dioscoreae esculentae and aqueous citric acid solution is 1:5 ~ 1:20.
3. the preparation method of high luminance relay valency purple sweet potato haematochrome according to claim 1 is characterized in that: ultrasonic frequency is 20 ~ 150KHz, and ultrasonic time is 10 ~ 120min.
4. according to the preparation method of claim 1 and 2 described high luminance relay valency purple sweet potato haematochromes, it is characterized in that: the mass concentration of aqueous citric acid solution is 0.45 ~ 0.55%, and the solid-to-liquid ratio of Rhizoma Dioscoreae esculentae and aqueous citric acid solution is 1:6 ~ 1:10.
5. according to the preparation method of claim 1 and 3 described high luminance relay valency purple sweet potato haematochromes, it is characterized in that: ultrasonic frequency is 50 ~ 60KHz, and ultrasonic time is 30 ~ 90min.
6. according to the preparation method of any one described high luminance relay valency purple sweet potato haematochrome in the claim 1 ~ 5; It is characterized in that: the centrifugal filter residue that obtains adds aqueous citric acid solution and carries out supersound extraction once more, behind the united extraction liquid after chromatography column, wherein; Ultrasonic frequency is 20 ~ 150KHz; Ultrasonic time is 10 ~ 120min, and the mass concentration of aqueous citric acid solution is 0.2 ~ 0.8%, and the solid-to-liquid ratio of Rhizoma Dioscoreae esculentae and aqueous citric acid solution is 1:2 ~ 1:8.
7. the preparation method of high luminance relay valency purple sweet potato haematochrome according to claim 6 is characterized in that: ultrasonic frequency is 50 ~ 60KHz, and ultrasonic time is 20 ~ 40min.
8. the preparation method of high luminance relay valency purple sweet potato haematochrome according to claim 6 is characterized in that: the mass concentration of aqueous citric acid solution is 0.45 ~ 0.55%, and the solid-to-liquid ratio of Rhizoma Dioscoreae esculentae and aqueous citric acid solution is 1:3 ~ 1:6.
9. the preparation method of high luminance relay valency purple sweet potato haematochrome according to claim 1 is characterized in that: the used resin model of chromatography column is AB-8 or 3520, and elutriant is 40 ~ 90% ethanolic soln.
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| CN2011102852233A CN102391668A (en) | 2011-09-23 | 2011-09-23 | Preparation method of purple sweet potato haematochrome with high color value |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105017799A (en) * | 2015-07-03 | 2015-11-04 | 上海应用技术学院 | Extraction method for purple potato haematochrome |
| CN105199426A (en) * | 2015-10-30 | 2015-12-30 | 湖南农业大学 | Method for extracting purple sweet potato pigments with natural amino acid phosphate aqueous solution |
| CN107525847A (en) * | 2016-11-09 | 2017-12-29 | 中国检验检疫科学研究院 | The quick determination method of colouring agent is disabled in a kind of wax crayon and Decal |
-
2011
- 2011-09-23 CN CN2011102852233A patent/CN102391668A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105017799A (en) * | 2015-07-03 | 2015-11-04 | 上海应用技术学院 | Extraction method for purple potato haematochrome |
| CN105199426A (en) * | 2015-10-30 | 2015-12-30 | 湖南农业大学 | Method for extracting purple sweet potato pigments with natural amino acid phosphate aqueous solution |
| CN105199426B (en) * | 2015-10-30 | 2017-08-25 | 湖南农业大学 | A kind of method that utilization natural amino acid aqueous phosphatic extracts purple potato pigment |
| CN107525847A (en) * | 2016-11-09 | 2017-12-29 | 中国检验检疫科学研究院 | The quick determination method of colouring agent is disabled in a kind of wax crayon and Decal |
| CN107525847B (en) * | 2016-11-09 | 2019-12-06 | 中国检验检疫科学研究院 | method for rapidly detecting forbidden colorant in crayon and water sticker |
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Application publication date: 20120328 |