CN102389149A - Method for extracting and purifying polyphenol from tender areca nuts - Google Patents
Method for extracting and purifying polyphenol from tender areca nuts Download PDFInfo
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- CN102389149A CN102389149A CN2011102877141A CN201110287714A CN102389149A CN 102389149 A CN102389149 A CN 102389149A CN 2011102877141 A CN2011102877141 A CN 2011102877141A CN 201110287714 A CN201110287714 A CN 201110287714A CN 102389149 A CN102389149 A CN 102389149A
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Abstract
The invention discloses a method for extracting and purifying polyphenol from tender areca nuts. The method comprises the following steps: (1) pulping the cleaned tender areca nuts, immediately adding hot de-ionized water for extracting, and then filtering to obtain a polyphenol coarse extraction liquid; and (2) adding the polyphenol coarse extraction liquid to a macroporous resin column, then eluting for separating and purifying. The polyphenol extracted from areca is a natural extract, has antioxidant efficacy, and can be applied to food and healthcare products.
Description
Technical field
The invention belongs to the method for extraction and purification of betel nut polyphenol, particularly a kind of method of from the tender fruit of betel nut, extracting the purifying polyphenol.
Background technology
Betel nut (Areca catechu L.) is China's traditional Chinese medicine material, has expelling parasite; It is long-pending to disappear; The treatment therapeutic method to keep the adverse qi flowing downward; Row water; Preventing malaria.Malnutrition due to parasitic infestation; Food stagnates; Abdominal distention; Weigh after rushing down dysentery; Beriberi; Oedema; Effects such as malaria.Pharmaceutical research proves that betel nut also has effects such as hypotensive, anticancer now, and China south also is used as food with betel nut like provinces such as Hainan, and a large amount of crowds chew the food betel nut.
Research shows that the betel nut polyphenol content is l7.2%～29.8% in green fruit period. arrived mature period and just dropped to 11.1～17.8%, so the present invention selects for use the green tender fruit of betel nut to extract polyphenol.
It is the anti-oxidant of pure natural that the present invention extracts the betel nut polyphenol that obtains, and can be used for food, health products by exploitation.
Summary of the invention
The objective of the invention is to make full use of the natural resources, a kind of method of from the tender fruit of betel nut, extracting the purifying polyphenol is provided.
Technical scheme of the present invention is summarized as follows:
From the tender fruit of betel nut, extract the method for purifying polyphenol, comprise the steps:
(1) the tender fruit of betel nut cleaned of deionized water is after the beater making beating; Add temperature in 5～15 minutes and be 70 ℃～90 ℃ deionized waters for extracting solvent, at 95 ℃, the feed liquid mass ratio is to extract 90 minutes under the condition of 1:5～10; Filter, get betel nut polyphenol crude extract;
(2) betel nut polyphenol crude extract is stirred; Last macroporous resin column; The concentration expressed in percentage by volume of using 8～15 times of column volume deionized waters, 3 times of column volumes is that the percentage concentration of 20%～40% ethanol water, 10 times of column volumes is that 95% ethanol water carries out wash-out, and collecting percentage concentration is 95% ethanol water wash-out liquid, is evaporated to pol 30%; Spray-drying promptly obtains the betel nut polyphenol behind the purifying.
After said extraction solvent should be the making beating of the tender fruit of betel nut, the temperature that adds in 5～15 minutes was 70 ℃～90 ℃ a deionized water.
Said solid-liquid ratio is 1:5～10.
Said macroreticular resin, model is: AB-8; HPD-100; ME-2; FL-1; D101; XAD-10; HP-20.
The specific embodiment
Explain that through concrete embodiment the present invention extracts the method for purifying polyphenol from the tender fruit of betel nut below, following description only is in order to make those skilled in the art understand the present invention better, but does not limit the present invention in any way.
Embodiment 1
From the tender fruit of betel nut, extract the method for purifying polyphenol, comprise the steps:
(1) the tender fruit of betel nut cleaned of deionized water is after the beater making beating, adds temperature in 5 minutes and be 70 ℃ of deionized waters for extracting solvent, and at 95 ℃, the feed liquid mass ratio is to extract 60 minutes under the condition of 1:5, filter, betel nut polyphenol crude extract;
(2) betel nut polyphenol crude extract is stirred; Last AB-8 macroporous resin column; The concentration expressed in percentage by volume of using 8 times of column volume deionized waters, 3 times of column volumes is that the percentage concentration of 20% ethanol water, 10 times of column volumes is that 95% ethanol water carries out wash-out, and collecting percentage concentration is 95% ethanol water wash-out liquid, is evaporated to pol 30%; Spray-drying promptly obtains the betel nut polyphenol behind the purifying.Polyphenol content 72%.
Embodiment 2
From the tender fruit of betel nut, extract the method for purifying polyphenol, comprise the steps:
(1) the tender fruit of betel nut cleaned of deionized water is after the beater making beating, adds temperature in 10 minutes and be 80 ℃ of deionized waters for extracting solvent, and at 95 ℃, the feed liquid mass ratio is to extract 60 minutes under the condition of 1:7, filter, betel nut polyphenol crude extract;
(2) betel nut polyphenol crude extract is stirred; Last HPD-100 macroporous resin column; The concentration expressed in percentage by volume of using 10 times of column volume deionized waters, 3 times of column volumes is that the percentage concentration of 30% ethanol water, 10 times of column volumes is that 95% ethanol water carries out wash-out, and collecting percentage concentration is 95% ethanol water wash-out liquid, is evaporated to pol 30%; Spray-drying promptly obtains the betel nut polyphenol behind the purifying.Polyphenol content 84%.
Embodiment 3
From the tender fruit of betel nut, extract the method for purifying polyphenol, comprise the steps:
(1) the tender fruit of betel nut cleaned of deionized water is after the beater making beating, adds temperature in 15 minutes and be 90 ℃ of deionized waters for extracting solvent, and at 95 ℃, the feed liquid mass ratio is to extract 60 minutes under the condition of 1:8, filter, betel nut polyphenol crude extract;
(2) betel nut polyphenol crude extract is stirred; Last ME-2 macroporous resin column; The concentration expressed in percentage by volume of using 12 times of column volume deionized waters, 3 times of column volumes is that the percentage concentration of 40% ethanol water, 10 times of column volumes is that 95% ethanol water carries out wash-out, and collecting percentage concentration is 95% ethanol water wash-out liquid, is evaporated to pol 30%; Spray-drying promptly obtains the betel nut polyphenol behind the purifying.Polyphenol content 87%.
Embodiment 4
From the tender fruit of betel nut, extract the method for purifying polyphenol, comprise the steps:
(1) the tender fruit of betel nut cleaned of deionized water is after the beater making beating, adds temperature in 7 minutes and be 75 ℃ of deionized waters for extracting solvent, and at 95 ℃, the feed liquid mass ratio is to extract 60 minutes under the condition of 1:10, filter, betel nut polyphenol crude extract;
(2) betel nut polyphenol crude extract is stirred; Last FL-1 macroporous resin column; The concentration expressed in percentage by volume of using 14 times of column volume deionized waters, 3 times of column volumes is that the percentage concentration of 25% ethanol water, 10 times of column volumes is that 95% ethanol water carries out wash-out, and collecting percentage concentration is 95% ethanol water wash-out liquid, is evaporated to pol 30%; Spray-drying promptly obtains the betel nut polyphenol behind the purifying.Polyphenol content 79%.
Embodiment 5
From the tender fruit of betel nut, extract the method for purifying polyphenol, comprise the steps:
(1) the tender fruit of betel nut cleaned of deionized water is after the beater making beating, adds temperature in 9 minutes and be 85 ℃ of deionized waters for extracting solvent, and at 95 ℃, the feed liquid mass ratio is to extract 60 minutes under the condition of 1:6, filter, betel nut polyphenol crude extract;
(2) betel nut polyphenol crude extract is stirred; Last D101 macroporous resin column; The concentration expressed in percentage by volume of using 15 times of column volume deionized waters, 3 times of column volumes is that the percentage concentration of 35% ethanol water, 10 times of column volumes is that 95% ethanol water carries out wash-out, and collecting percentage concentration is 95% ethanol water wash-out liquid, is evaporated to pol 30%; Spray-drying promptly obtains the betel nut polyphenol behind the purifying.Polyphenol content 83%.
Embodiment 6
From the tender fruit of betel nut, extract the method for purifying polyphenol, comprise the steps:
(1) the tender fruit of betel nut cleaned of deionized water is after the beater making beating, adds temperature in 12 minutes and be 90 ℃ of deionized waters for extracting solvent, and at 95 ℃, the feed liquid mass ratio is to extract 60 minutes under the condition of 1:9, filter, betel nut polyphenol crude extract;
(2) betel nut polyphenol crude extract is stirred; Last XAD-10 macroporous resin column; The concentration expressed in percentage by volume of using 13 times of column volume deionized waters, 3 times of column volumes is that the percentage concentration of 32% ethanol water, 10 times of column volumes is that 95% ethanol water carries out wash-out, and collecting percentage concentration is 95% ethanol water wash-out liquid, is evaporated to pol 30%; Spray-drying promptly obtains the betel nut polyphenol behind the purifying.Polyphenol content 70%.
Embodiment 7
From the tender fruit of betel nut, extract the method for purifying polyphenol, comprise the steps:
(1) the tender fruit of betel nut cleaned of deionized water is after the beater making beating, adds temperature in 8 minutes and be 85 ℃ of deionized waters for extracting solvent, and at 95 ℃, the feed liquid mass ratio is to extract 60 minutes under the condition of 1:8, filter, betel nut polyphenol crude extract;
(2) betel nut polyphenol crude extract is stirred; Last HP-20 macroporous resin column; The concentration expressed in percentage by volume of using 11 times of column volume deionized waters, 3 times of column volumes is that the percentage concentration of 27% ethanol water, 10 times of column volumes is that 95% ethanol water carries out wash-out, and collecting percentage concentration is 95% ethanol water wash-out liquid, is evaporated to pol 30%; Spray-drying promptly obtains the betel nut polyphenol behind the purifying.Polyphenol content 79%.
Embodiment 8
From the tender fruit of betel nut, extract the method for purifying polyphenol, comprise the steps:
(1) the tender fruit of betel nut cleaned of deionized water is after the beater making beating, adds temperature in 6 minutes and be 90 ℃ of deionized waters for extracting solvent, and at 95 ℃, the feed liquid mass ratio is to extract 60 minutes under the condition of 1:10, filter, betel nut polyphenol crude extract;
(2) betel nut polyphenol crude extract is stirred; Last D101 macroporous resin column; The concentration expressed in percentage by volume of using 15 times of column volume deionized waters, 3 times of column volumes is that the percentage concentration of 30% ethanol water, 10 times of column volumes is that 95% ethanol water carries out wash-out, and collecting percentage concentration is 95% ethanol water wash-out liquid, is evaporated to pol 30%; Spray-drying promptly obtains the betel nut polyphenol behind the purifying.Polyphenol content 88%.
Embodiment 9
From the tender fruit of betel nut, extract the method for purifying polyphenol, comprise the steps:
(1) the tender fruit of betel nut cleaned of deionized water is after the beater making beating, adds temperature in 10 minutes and be 80 ℃ of deionized waters for extracting solvent, and at 95 ℃, the feed liquid mass ratio is to extract 60 minutes under the condition of 1:8, filter, betel nut polyphenol crude extract;
(2) betel nut polyphenol crude extract is stirred; Last ME-2 macroporous resin column; The concentration expressed in percentage by volume of using 9 times of column volume deionized waters, 3 times of column volumes is that the percentage concentration of 20% ethanol water, 10 times of column volumes is that 95% ethanol water carries out wash-out, and collecting percentage concentration is 95% ethanol water wash-out liquid, is evaporated to pol 30%; Spray-drying promptly obtains the betel nut polyphenol behind the purifying.Polyphenol content 73%.
Claims (5)
1. from the tender fruit of betel nut, extract the method for purifying polyphenol, it is characterized in that comprising the steps:
(1) the tender fruit of betel nut cleaned of deionized water is after the beater making beating; Add temperature in 5～15 minutes and be 70 ℃～90 ℃ deionized waters for extracting solvent, at 95 ℃, the feed liquid mass ratio is to extract 90 minutes under the condition of 1:5～10; Filter, get betel nut polyphenol crude extract;
(2) betel nut polyphenol crude extract is stirred; Last macroporous resin column; The concentration expressed in percentage by volume of using 8～15 times of column volume deionized waters, 3 times of column volumes is that the percentage concentration of 20%～40% ethanol water, 10 times of column volumes is that 95% ethanol water carries out wash-out, and collecting percentage concentration is 95% ethanol water wash-out liquid, is evaporated to pol 30%; Spray-drying promptly obtains the betel nut polyphenol behind the purifying.
2. from the tender fruit of betel nut, extract the method for purifying polyphenol according to claim 1, it is characterized in that said raw material is the green tender fruit of betel nut.
3. from the tender fruit of betel nut, extract the method for purifying polyphenol according to claim 1, it is characterized in that the tender fruit of betel nut after the beater making beating, adding temperature in 5～15 minutes is that 70 ℃～90 ℃ deionized waters are for extracting solvent.
4. according to the said method of from the tender fruit of betel nut, extracting the purifying polyphenol of claim 1, it is characterized in that said solid-liquid ratio is 1:5～10.
5. according to the said method of from the tender fruit of betel nut, extracting the purifying polyphenol of claim 1, it is characterized in that said macroreticular resin is AB-8; HPD-100; ME-2; FL-1; D101; XAD-10; HP-20.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478719A (en) * | 2013-09-27 | 2014-01-01 | 许启太 | Method for extracting betel nut biotin form fresh betel nuts |
| CN106417843A (en) * | 2016-09-30 | 2017-02-22 | 许启太 | Betel nut and peony seed coffee and preparation method thereof |
| CN107582782A (en) * | 2017-10-19 | 2018-01-16 | 王荣 | A kind of process for preparing medicine of composition containing betel nut and its anti anoxia application |
| CN108516995A (en) * | 2018-04-27 | 2018-09-11 | 湖南口味王集团有限责任公司 | A kind of method of purification of betel nut polyphenol |
| CN108606029A (en) * | 2018-04-28 | 2018-10-02 | 洛阳师范学院 | A kind of coarse grain biscuit and preparation method thereof with anti-fatigue effect |
| CN109123764A (en) * | 2018-07-23 | 2019-01-04 | 许启太 | A kind of preparation method of Bin phenol cigarette |
| CN109674916A (en) * | 2019-03-05 | 2019-04-26 | 海南口味王科技发展有限公司 | A method of preparing betelnut extract from fresh areca nuts |
| CN110496179A (en) * | 2019-09-26 | 2019-11-26 | 海南大学 | A kind of method and application for rapidly and efficiently extracting antioxidant in Areca |
| CN111869841A (en) * | 2020-07-16 | 2020-11-03 | 海南华创槟榔研究院 | Betel nut chewable tablet and preparation method thereof |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478719A (en) * | 2013-09-27 | 2014-01-01 | 许启太 | Method for extracting betel nut biotin form fresh betel nuts |
| CN103478719B (en) * | 2013-09-27 | 2015-12-02 | 许启太 | A kind of method extracting betel nut biotin from fresh betel nut |
| CN106417843A (en) * | 2016-09-30 | 2017-02-22 | 许启太 | Betel nut and peony seed coffee and preparation method thereof |
| CN107582782A (en) * | 2017-10-19 | 2018-01-16 | 王荣 | A kind of process for preparing medicine of composition containing betel nut and its anti anoxia application |
| CN107582782B (en) * | 2017-10-19 | 2020-11-03 | 中国人民解放军联勤保障部队第九〇四医院 | Preparation method of medicine containing areca catechu component and anti-hypoxia application of medicine |
| CN108516995A (en) * | 2018-04-27 | 2018-09-11 | 湖南口味王集团有限责任公司 | A kind of method of purification of betel nut polyphenol |
| CN108606029A (en) * | 2018-04-28 | 2018-10-02 | 洛阳师范学院 | A kind of coarse grain biscuit and preparation method thereof with anti-fatigue effect |
| CN109123764A (en) * | 2018-07-23 | 2019-01-04 | 许启太 | A kind of preparation method of Bin phenol cigarette |
| CN109674916A (en) * | 2019-03-05 | 2019-04-26 | 海南口味王科技发展有限公司 | A method of preparing betelnut extract from fresh areca nuts |
| CN110496179A (en) * | 2019-09-26 | 2019-11-26 | 海南大学 | A kind of method and application for rapidly and efficiently extracting antioxidant in Areca |
| CN111869841A (en) * | 2020-07-16 | 2020-11-03 | 海南华创槟榔研究院 | Betel nut chewable tablet and preparation method thereof |
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| CN102389149B (en) | 2016-04-13 |
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