CN102388895A - Holothurin bactericidal growth promotion agent - Google Patents
Holothurin bactericidal growth promotion agent Download PDFInfo
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- CN102388895A CN102388895A CN2011102915675A CN201110291567A CN102388895A CN 102388895 A CN102388895 A CN 102388895A CN 2011102915675 A CN2011102915675 A CN 2011102915675A CN 201110291567 A CN201110291567 A CN 201110291567A CN 102388895 A CN102388895 A CN 102388895A
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- holothurin
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- surfactant
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- sterilizing
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Abstract
The invention discloses a holothurin bactericidal growth promotion agent. The holothurin bactericidal growth promotion agent comprises the following components with the contents: 50 to 100 ppm of holothurin, 100 to 150 ppm of salicyl saponin, 5 to 10 ppm of surfactant OP, 15 to 20 ppm of heteroauxin, 5 to 20 ppm of exogenous hormones and a small quantity of Zn (Zinc), Fe (Ferrum), Mn (Manganese) and H3BO3, and the use concentration ranges from 5000 to 1000 ppm; the surfactant is preferably the wetting agent on sale. After the components are proportioned according to the above contents, the holothurin bactericidal growth promotion agent provided by the invention is used for plant growth, has the advantages of nutrition supply, bacteria killing, the benefit of pollination of flowers and improvement in utilization ratio of fertilizer and reduction of the dosage of pesticide and the like.
Description
Technical field
The present invention relates to a kind of short long agent, be specifically related to a kind of holothurin agent for sterilizing and promoting growth.
Background technology
The various countries scholar has carried out wide chemical and pharmacological research to the sea cucumber of different cultivars in recent years, and we are separated to holothurin from the hojothuria leucospilota internal organ extraction of fresh collection, prove antineoplastic antifungic action.Be used to treat dermatophytosis clinically, obtain curative effect preferably, produce with the clean trade name of the tinea that disappears at present.Yet, aspect plant growing, using seldom for holothurin, even do not having.
Summary of the invention
To the weak point of above-mentioned prior art, the present invention aims to provide a kind of holothurin agent for sterilizing and promoting growth.
Technical scheme of the present invention: the holothurin agent for sterilizing and promoting growth, the component and the content of said short long agent comprise: holothurin 50~100ppm, bigcatkin willow saponin 100~150ppm, heteroauxin 15~20ppm, mepiquat chloride 5~10ppm, surfactant 0P are 5~10ppm.
Further, the component and the content of said short long agent comprise: holothurin 60~90ppm, and bigcatkin willow saponin 110~140ppm, heteroauxin 16~19ppm, mepiquat chloride 6~9ppm, surfactant OP are 6~9ppm.
Further, the component and the content of said short long agent comprise: holothurin 70~80ppm, and bigcatkin willow saponin 120~130ppm, heteroauxin 17~18ppm, mepiquat chloride 7~8ppm, surfactant OP are 7~8ppm.
Further, said surfactant is commercially available wetting agent.
Further, said short long agent also includes a small amount of Zn, Fe, Mn, H
3BO
3
Beneficial effect of the present invention: through above-mentioned component according to quantity after the proportioning, is used in the plant growing, have extra-nutrition, fungus killing is short long, helps the pollination of flower, improves fertilizer utilization ratio, reduces advantages such as pesticide dosage.
Embodiment
Further specify the present invention below in conjunction with embodiment.
The component and the content of described holothurin agent for sterilizing and promoting growth comprise: holothurin 50~100ppm, bigcatkin willow saponin 100~150ppm, surfactant OP are 5~10ppm, heteroauxin 15~20ppm, mepiquat chloride 5~10ppm and macroelement Zn, Fe, Mn, H
3BO
3, its working concentration is 500~1000ppm.
Further, the component and the content of said short long agent comprise: holothurin 60~90ppm, and bigcatkin willow saponin 110~140ppm, heteroauxin 16~19ppm, mepiquat chloride 6~9ppm, surfactant OP are 6~9ppm.
Further, the component and the content of said short long agent comprise: holothurin 70~80ppm, and bigcatkin willow saponin 120~130ppm, heteroauxin 17~18ppm, mepiquat chloride 7~8ppm, surfactant OP are 7~8ppm.
Preferably, said surfactant is commercially available wetting agent.
For holothurin: will remove the sea cucumber internal organ of clean silt, and after shredding, smash (10000 rev/mins * 3 minutes) to pieces, with 70-80% ethanol cold soaking 3 times with the high speed bruisher; Each 1 all left and right sides time, decompression recycling ethanol to medicinal extract shape (brownish red) with methyl alcohol repeated multiple times dissolving medicinal extract, is removed insoluble matter; Methyl alcohol component recovered under reduced pressure methyl alcohol medicinal extract shape, multiplexing water-soluble separating, the elimination insoluble matter, the aqueous solution is with Petroleum ether extraction 3 times; The aqueous solution after the degreasing extracts several times with n-butanol, merges n-butanol layer, uses anhydrous sodium sulfate drying, filters and removes sodium sulphate; The recovered under reduced pressure n-butanol gets pale yellow powder to doing, and claims holothurin.This material has tangible aceticanhydride, belongs to oside compound.
Sea cucumber have stronger antifungic action, when concentration is 10-40 μ g/ml, and to achorion, trichophyton tonsurans, Cryptococcus neoformans etc. all have the growth of inhibition effect.Try out in treatment dermatophyte 113 examples, obtain curative effect preferably.
Bigcatkin willow saponin itself is a growth regulator, pharmaceutically can be bactericide.
Said surfactant is the molecule that is formed by two kinds of distinct particles, and a kind of particle has extremely strong lipophile, and is another kind of then have extremely strong hydrophily.After being dissolved in the water, surfactant can reduce the surface tension of water, and improves the solubility of organic compound.
Said heteroauxin is as plant growth substance and analytical reagent.Auxin class materials such as 3-indolyl acetic acid and 3-indoleacetaldehyde, 3-indole acetonitrile, ascorbic acid are in the natural existence of natural world, and 3-indolyl acetic acid biosynthetic precursor in plant corpus is a tryptophan.The basic role of auxin is to regulate the growth of plant, can not only promote growth, and has the effect that inhibition is grown and organ builds up.Auxin not only exists with free state in plant cell; Can also exist with the bound form auxin of strong bonded such as boiomacromolecule; The auxin that forms bond with particular matter is also arranged, for example indoles acetyl group asparagine, heteroauxin arabinose and indoles acetyl glucosamine etc.This possibly be auxin at intracellular a kind of storage method, also be to remove the detoxifcation mode that superfluous auxin is poisoned.
Said mepiquat chloride has retarding action to vegetation growth of plant, and mepiquat chloride can absorb through plant leaf and root, conducts to complete stool; Can reduce the activity of plant body inner gibberellin, thereby suppress cell elongation, the terminal bud growing way weakens, and the control plant grows in length and breadth; The plant internode is shortened, and plant type is compact, and the leaf color depth is thick, and leaf area reduces; And strengthen chlorophyllous synthesizing, can prevent the prosperous length of plant, postpone Feng Hang etc.Mepiquat chloride can improve cell membrane stability, increases plant resistance.
The preparation method of this holothurin agent for sterilizing and promoting growth is:
A. with after the required chemical raw material roughing, chemically examine, selected;
B. with said holothurin, bigcatkin willow saponin, surfactant, heteroauxin, mepiquat chloride, mix according to quantity with water and to blend, and then add and contain Zn, Fe, Mn, H
3Bo
3Isoreactivity thing or compound can make said holothurin agent for sterilizing and promoting growth.
C. the holothurin agent for sterilizing and promoting growth of processing is divided in the plastic bottle, absorbs as required during use and get final product.
This holothurin agent for sterilizing and promoting growth can be used for grain, cotton, oil, fiber crops, melon and fruit, greengrocery.
Embodiment 1: the component of said holothurin agent for sterilizing and promoting growth and content are: holothurin 50ppm, bigcatkin willow saponin 100ppm, surfactant OP are 5ppm, heteroauxin 15ppm, mepiquat chloride 5ppm and macroelement Zn, Fe, Mn, H
3BO
3, its working concentration is 500ppm.
Embodiment 2: the component of said holothurin agent for sterilizing and promoting growth and content are: holothurin 60ppm, bigcatkin willow saponin 110ppm, surfactant OP are 6ppm, heteroauxin 16ppm, mepiquat chloride 6ppm and macroelement Zn, Fe, Mn, H
3BO
3, its working concentration is 600ppm.
Embodiment 3: the component of said holothurin agent for sterilizing and promoting growth and content are: holothurin 70ppm, bigcatkin willow saponin 120ppm, surfactant OP are 7ppm, heteroauxin 17ppm, mepiquat chloride 7ppm and macroelement Zn, Fe, Mn, H
3BO
3, its working concentration is 700ppm.
Embodiment 4: the component of said holothurin agent for sterilizing and promoting growth and content are: holothurin 80ppm, bigcatkin willow saponin 130ppm, surfactant OP are 8ppm, heteroauxin 18ppm, mepiquat chloride 8ppm and macroelement Zn, Fe, Mn, H
3BO
3, its working concentration is 800ppm.
Embodiment 5: the component of said holothurin agent for sterilizing and promoting growth and content are: holothurin 90ppm, bigcatkin willow saponin 140ppm, surfactant OP are 9ppm, heteroauxin 19ppm, mepiquat chloride 9ppm and macroelement Zn, Fe, Mn, H
3BO
3, its working concentration is 900ppm.
Embodiment 6: the component of said holothurin agent for sterilizing and promoting growth and content are: holothurin 100ppm, bigcatkin willow saponin 150ppm, surfactant OP are 10ppm, heteroauxin 20ppm, mepiquat chloride 10ppm and macroelement Zn, Fe, Mn, H
3BO
3, its working concentration is 1000ppm.
In practice; Each short long agent according to above embodiment 1 to embodiment 6 preparation is used grain, cotton, oil, fiber crops, melon and fruit respectively; Patient's condition such as withered, that Huang withers do not appear in said grain, cotton, oil, fiber crops, melon and fruit in growing way; And the grain, cotton, oil, fiber crops, melon and fruit etc. that use other agricultural chemicals the same period all have in various degree withered, patient's condition such as Huang withers; And the present invention than other agricultural chemicals in use, and the output of each crop has all obtained corresponding raising, and the damping off of vegetables is also had special efficacy.
The present invention's ability controlling plant tissue growth, growth can suppress the elongation of stem, make stem body X direction enlarge, increase slightly, effectively control the formation of carpopodium absciss layer, prevent coming off of organ.
Claims (5)
1. the holothurin agent for sterilizing and promoting growth is characterized in that, the component and the content of said short long agent comprise: holothurin 50~100ppm, and bigcatkin willow saponin 100~150ppm, heteroauxin 15~20ppm, mepiquat chloride 5~10ppm, surfactant OP are 5~10ppm.
2. holothurin agent for sterilizing and promoting growth according to claim 1; It is characterized in that: the component and the content of said short long agent comprise: holothurin 60~90ppm, bigcatkin willow saponin 110~140ppm, heteroauxin 16~19ppm; Mepiquat chloride 6~9ppm, surfactant OP are 6~9ppm.
3. holothurin agent for sterilizing and promoting growth according to claim 1; It is characterized in that: the component and the content of said short long agent comprise: holothurin 70~80ppm, bigcatkin willow saponin 120~130ppm, heteroauxin 17~18ppm; Mepiquat chloride 7~8ppm, surfactant OP are 7~8ppm.
4. according to the described holothurin agent for sterilizing and promoting growth of any claim of claim 1 to 3, it is characterized in that: said surfactant is commercially available wetting agent.
5. holothurin agent for sterilizing and promoting growth according to claim 1 is characterized in that: said short long agent also includes a small amount of Zn, Fe, Mn, H
3BO
3
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CN2011102915675A CN102388895A (en) | 2011-09-30 | 2011-09-30 | Holothurin bactericidal growth promotion agent |
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Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009085216A2 (en) * | 2007-12-20 | 2009-07-09 | Squicor | Compositions and methods for detecting or elimninating senescent cells to diagnose or treat disease |
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Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009085216A2 (en) * | 2007-12-20 | 2009-07-09 | Squicor | Compositions and methods for detecting or elimninating senescent cells to diagnose or treat disease |
Non-Patent Citations (4)
Title |
---|
《中国海洋大学学报》 20061130 丛日山 等 "仿刺参水溶性海参皂苷的分离制备及抗真菌活性的研究" 第962页表2 1-5 第36卷, 第6期 * |
《广东医学》 19890501 周清凯 等 "海参素及其抗肿瘤真菌的作用" 第40页 1-5 , 第4期 * |
丛日山 等: ""仿刺参水溶性海参皂苷的分离制备及抗真菌活性的研究"", 《中国海洋大学学报》 * |
周清凯 等: ""海参素及其抗肿瘤真菌的作用"", 《广东医学》 * |
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