CN102369998A - Method for preparing easy-to-absorb low-lactose dairy product rich in galacto-oligosaccharide - Google Patents
Method for preparing easy-to-absorb low-lactose dairy product rich in galacto-oligosaccharide Download PDFInfo
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- CN102369998A CN102369998A CN2010102553330A CN201010255333A CN102369998A CN 102369998 A CN102369998 A CN 102369998A CN 2010102553330 A CN2010102553330 A CN 2010102553330A CN 201010255333 A CN201010255333 A CN 201010255333A CN 102369998 A CN102369998 A CN 102369998A
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Abstract
Currently, nutritive values are a main pursuit of dairy products in markets, and functional pursuits are not commonly assigned on dairy products. Even if a dairy product is claimed to be with special functionalities, the efficacies are originated from additional materials but not intrinsic components of the dairy product. Although a small amount of dairy product has a pursuit of low lactose, a product with both low lactose and high galacto-oligosaccharide is not found. Compared to a method with additional materials, the method provided by the invention is advantaged in low cost. The invention relates to a method for preparing an easy-to-absorb low-lactose dairy product rich in galacto-oligosaccharide. The method is characterized in that: a milk raw material is directly processed by using lactase. The invention also relates to a functional dairy product prepared with the method provided by the invention. The content of galacto-oligosaccharide reaches an efficacy level.
Description
Technical field
The present invention relates to a kind of preparation and be rich in galactooligosaccharide and the easy method of dairy products and the dairy products that are rich in galactooligosaccharide that prepare with said method of absorbing of low lactose.
Background technology
For a long time; Milk is the popular high-nutrition food of being familiar with already; Comprise various important nutrient such as protein, fat, amino acid, minerals and vitamins in the milk constituents, show that in medical science, the existing multinomial result of study of nutrition educational circles many drink milks can prevention of osteoporosis run off.
But milk protein also is difficult for by human consumption and absorption, and casein is a macro-molecular protein in the contained protein of milk, must pass through human chitinase and resolve into little molecule, just is prone to digest and assimilate.And the sugar in the milk is lactose (lactose); Human body reduces lactose if can't secrete enough lactases; Bacterium can become lactic acid, carbon dioxide and other organic acid by lactose fermenters in the enteron aisle; Can cause enteron aisle osmotic pressure uneven, cause lactose intolerance (lactose intolerance), produce symptoms such as diarrhoea, stomachache, abdominal distension.
Galactooligosaccharide is known can be reduced enteron aisle pH value and promote intestines peristalsis; The hyperplasia that suppresses harmful bacterium, and reduce the toxic waste that harmful bacterium metabolism is produced, have environmental protection in the body of keeping; The effect of activating human body function is a kind of important functional composition in breast milk and the cow's milk.
United States Patent (USP) the 5th, 032 discloses a kind of method for preparing galactooligosaccharide in No. 509, and said method is with beta galactosidase (β-galactosidase) and glucose isomerase (glucose isomerase) processing with lactose.With the lactose of the solid content of 60% (w/w) in 70 ℃ of pH values 4.5, temperature through beta galactosidase effect 2 hours; Generate galactooligosaccharide; Again in 7.5,60 ℃ of pH values with glucose isomerase effect 16 hours, convert lactose hydrolysate glucose to fructose.Said method makes 60% (w/w) syrup, comprises 28.4% galactooligosaccharide, 52.4% disaccharide, 8.0% glucose, 8.1% fructose and 3.1% galactolipin, can be used as sugar replacement or food additives.
United States Patent (USP) the 5th, 378 also discloses a kind of method for preparing galactooligosaccharide No. 833; Said method is with lactose and galactolipin, and ratio is about 9: 1 to 5: 5, preferably is about 8: 2 to 7: 3; With inorganic acid,, handled 0.5 to 3 hour under 100～200 ℃ of the high temperature like hydrochloric acid, sulfuric acid, nitric acid and phosphoric acid etc.; Be preferably 1 to 2 hour, can obtain 80% above galactooligosaccharide conversion ratio, via neutralization, decolouring, desalination, concentrate and spray-drying after process powdery product.
The TaiWan, China patent discloses a kind of method for preparing low lactose, LG dairy products No. 095130588; It is to use yeast or lactase to handle; Under 15～35 ℃ of temperature, carry out fermentation step; Last 10～48 hours, lactose content is reduced to 50% of the lactose content that is lower than said initial substance, and said glucose content is reduced to 50% of the glucose amount that is lower than said initial substance.The gained dairy products have the saccharomycetes to make fermentation local flavor, in case of necessity, need to add flavoring to shelter this not good local flavor.
In addition; Disclose a kind of preparation in No. the 1903052nd, the Chinese patent and have the method for the whey powder of CPP, peptide resisting angiotensin-convertion enzyme and galactooligosaccharide; It is to be raw material with the mammalian milk, through lactobacillus-fermented, adds renin and forms curdled milk; Warp heating, fragmentation also filter to isolate whey; Obtain protein content 75～90% condensed whey liquid after concentrating, whey liquid acts on 1～4 hour with trypsase, pepsin and galactosidase under 35～60 ℃ of temperature conditions, and enzyme goes out; Vacuum drying under 40～50 ℃ of conditions obtains the hydrolysis condensed whey of total solid 40～50%, carries out spray-drying again, obtains having CPP, the whey powder finished product of peptide resisting angiotensin-convertion enzyme and galactooligosaccharide.
Moreover Chinese patent discloses the synthetic method of 3,6 branching compound sugar of a kind of galactolipin for No. 1349998; It is with 1,2,5; 6-two-O-isopropylidene-α-D-gala furanose is the compound sugar that the initiation material preparation has 3,6 branching of galactolipin of important biomolecule function.
The aforementioned method for preparing galactooligosaccharide all belongs to chemical synthesis process, and the processing step of the aforementioned preparation method of part is loaded down with trivial details and saccharomycete and enzyme processing time are long, so that production cost is higher.This skill is needed a kind of synthetic method non-chemically that can effectively generate galactooligosaccharide badly.
Moreover in the galactooligosaccharide content of the method for not having arbitrary Prior Art in improving dairy products, other that also promote said dairy products is functional.For example, improve in the dairy products galactooligosaccharide content and reduce lactose content and avoid lactose intolerance simultaneously.
Summary of the invention
The present invention proposes a kind of enzyme decomposition method in view of the above, and it can generate the dairy products that are rich in high unit galactooligosaccharide.Enzyme decomposition method of the present invention also can reduce the lactose content in the dairy products simultaneously.
One of the object of the invention provides a kind of enzyme decomposition method, wherein is to use lactase (lactase), converts the lactose in the dairy milk starting material to galactooligosaccharide, has the dairy products of high unit galactooligosaccharide with generation.
This paper alleged " dairy milk starting material " can be from any mammal species, and it includes but not limited to the dairy milk starting material from ox, goat or sheep.Preferred person is ox, goat or sheep breast, and better person is a cow's milk.The used breast of the inventive method can carry out upgrading before handling, for example, can convert thereof into skimmed milk, low fat milk, lactalbumin, whey, serum albumen, lactoferrin, lactose.Therefore, this paper alleged " dairy milk starting material " also comprises skimmed milk, low fat milk, lactalbumin, whey, serum albumen, lactoferrin, lactose.
The employed dairy milk starting material of the inventive method can pass through concentration and is the high concentration state.It is the dairy milk starting material of 14% (w/w) that a specific embodiment of the present invention is to use solid content; It is the dairy milk starting material of 40% (w/w) that another specific embodiment of the present invention is to use solid content.The dairy milk starting material that the present invention uses comprises the dairy milk starting material of solid content about 13～60% (w/w).Preferred person has about 14～40% (w/w).
Can breast be processed albumen, cow's milk or milk powder via dried, and before using the inventive method to handle, be dissolved in the water, use as dairy milk starting material.For example, can albumen, cow's milk or milk powder be dissolved in the water.
The inventive method can be used the lactase in any source, and it includes but not limited to the lactase from Aspergillus (Aspergillus), saccharomycete (Saccharomyces), Kluyveromyces sp (Kluyveromyces).Preferred person is beta galactosidase (β-galactosidase).
The inventive method can optionally use extra enzyme differential to separate the dairy milk starting material of handling through lactase, so that the gained dairy products obtain further function.For example, can use protease (protease), change wherein protein transduction into amino acid, be beneficial to the absorption of milk protein, reduce allergic reaction.
In view of the above; Another object of the present invention; Provide a kind of pair of enzyme differential and separate method; Wherein be to use lactase to convert the lactose in the dairy milk starting material to galactooligosaccharide, and change protein transduction into amino acid, have high unit galactooligosaccharide and the dairy products that reduce the casein anaphylactogen with generation with protease.
The inventive method can be used the protease in any source, and it includes but not limited to flavor protease (flavourzyme), and fungal proteinase, like aspergillus oryzae (Aspergillus oryze).
Enzyme decomposition reaction of the present invention can be carried out according to the known enzyme reaction condition of technical field of the present invention.Like the those skilled in the art person of understanding in the technical field of the present invention; Conditions such as the employed enzyme amount of the inventive method, operating temperature and operating time; Can be by those skilled in the art in the technical field of the present invention, according to employed dairy milk starting material, employed enzyme and required judgement decisions such as end-product.
Be to use the lactase of about 0.1～0.5% (w/w) according to the method for the invention.Preferred person is to use the lactase of about 0.2～0.3% (w/w).Be to use the protease of about 0.1～0.5% (w/w) according to the method for the invention.Preferred person is to use the protease of about 0.2～0.3% (w/w).
Enzyme decomposition reaction according to the present invention is under 30～60 ℃, to carry out.Preferred person is under 40～50 ℃, to carry out.
Enzyme differential according to the present invention is separated reaction and was carried out 30～120 minutes.Preferred person carried out 60～90 minutes.
After accomplishing enzyme and handling, method that can convention is ended enzyme reaction.For example, can the breast of gained be heated the enzyme that goes out, cooling subsequently.About 60～90 ℃ of heating enzyme-removal temperature, preferred person is heated to about 70～80 ℃.The enzyme that goes out is cooled to about 20 ℃ after handling, and preferred person reduces to about 10 ℃.
At last, the product behind the enzyme that goes out can convention be handled the method sterilization of dairy products.For example, can use pasteurizing or UHT superhigh temperature instantaneously sterilising.
Optionally, can use aseptic cold filling technology (aseptic cooling filler system) packing gained end-product.
According to the enzyme of the inventive method gained handle newborn product (solid content 8～60%w/w) contains the galactooligosaccharide of have an appointment 0.3～8% (w/w), and final liquid diary product (solid content 7～28%w/w) contains the galactooligosaccharide of have an appointment 0.2～5% (w/w).Preferred person, the enzyme of the inventive method gained handle newborn product (solid content 8～60%w/w) contains the galactooligosaccharide of have an appointment 2～6% (w/w), and final liquid diary product (solid content 7～28%w/w) contains the galactooligosaccharide of have an appointment 0.5～3% (w/w).
Handle newborn product (solid content 40%w/w) according to the enzyme of the inventive method gained and contain lactose and be lower than about 4% (w/w), and final liquid diary product (solid content 12%w/w) contains lactose and is lower than about 1.5% (w/w).Preferred person, enzyme is handled newborn product (solid content 40%w/w) and is contained lactose and be lower than about 3% (w/w), and final liquid diary product (solid content 12%w/w) contains lactose and is lower than about 1% (w/w).
In view of the above, another purpose of the present invention provides a kind of galactooligosaccharide and easy dairy products that absorb of low lactose of being rich in, and it is made by the inventive method.
Dairy products of the present invention can be made into the breast drink or the dairy products of normal temperature kenel, also can be made into the breast drink or the dairy products of refrigeration.It also can be used as the substrate of making ice cream, milk shake, flavored milk, sour milk, health drink, snack food, soup article or snack food.
The specific embodiment
Following examples are to be used for the present invention is further specified, but non-in order to limit scope of the present invention.
The condition of instance 1 to 8 and galactooligosaccharide content are shown in table one.
Instance 1
Raw milk (solid content 12%w/w) is (CNS3056) with contained lactose yield, and the speed of every 100g lactose 0.1g is added from the lactase of Aspergillus (Aspergillus) in wherein.Then reacted 0 to 24 hour down in 4 ℃.Gained breast product had the galactooligosaccharide content of 0,0.13 (g/100g) respectively in the 0th, 24 hour.
Instance 2
Raw milk (solid content 12%w/w) is (CNS3056) with contained lactose yield, and the speed of every 100g lactose 0.1g is added from the lactase of Aspergillus (Aspergillus) in wherein.Then reacted 0 to 2 hour down in 50 ℃.Gained breast product had the galactooligosaccharide content of 0,0.285 (g/100g) respectively in the 0th, 2 hour.
Instance 3
Milk powder stirred form high concentration cow's milk (solid content 14%w/w) in the water that is dissolved in 55 ℃.Speed with every 100g lactose 0.1g is added the lactase from Aspergillus (Aspergillus).Then reacted 0 to 120 minute down in 50 ℃.Gained breast product had the galactooligosaccharide content of 0,2.2,4.86,4.65 (g/100g) respectively in the 0th, 30,60,120 minute.
Instance 4
Milk powder stirred form high concentration cow's milk (solid content 40%w/w) in the water that is dissolved in 55 ℃.Speed with every 100g lactose 0.1g is added the lactase from Aspergillus (Aspergillus).Then reacted 0 to 120 minute down in 50 ℃.Gained breast product had the galactooligosaccharide content of 0,1.08,1.16,0.85 (g/100g) respectively in the 0th, 30,60,120 minute.
Instance 5
Milk powder stirred form high concentration cow's milk (solid content 14%w/w) in the water that is dissolved in 55 ℃.Speed with every 100g lactose 0.1g is added the lactase from saccharomycete (Saccharomyces).Then reacted 0 to 120 minute down in 50 ℃.Gained breast product had the galactooligosaccharide content of 0,3.17,4.3,4.9 (g/100g) respectively in the 0th, 30,60,120 minute.
Instance 6
Milk powder stirred form high concentration cow's milk (solid content 40%w/w) in the water that is dissolved in 55 ℃.Speed with every 100g lactose 0.1g is added the lactase from saccharomycete (Saccharomyces).Then reacted 0 to 120 minute down in 50 ℃.Gained breast product had the galactooligosaccharide content of 0,1.01,0.89,0.84 (g/100g) respectively in the 0th, 30,60,120 minute.
Instance 7
Milk powder stirred form high concentration cow's milk (solid content 14%w/w) in the water that is dissolved in 55 ℃.Speed with every 100g lactose 0.1g is added the lactase from Kluyveromyces sp (Kluyveromyces).Then reacted 0 to 120 minute down in 50 ℃.Gained breast product had the galactooligosaccharide content of 0,4.3,5.19,5.07 (g/100g) respectively in the 0th, 30,60,120 minute.
Instance 8
Milk powder stirred form high concentration cow's milk (solid content 40%w/w) in the water that is dissolved in 55 ℃.Speed with every 100g lactose 0.1g is added the lactase from Kluyveromyces sp (Kluyveromyces).Then reacted 0 to 120 minute down in 50 ℃.Gained breast product had the galactooligosaccharide content of 0,1.12,1.53,0.96 (g/100g) respectively in the 0th, 30,60,120 minute.
The reaction condition of instance 1-8 and galactooligosaccharide content are as shown in the table:
[table]
Liquid breast with instance 1 to 8 gained heats the enzyme (70～80 ℃ of temperature) that goes out, and is cooled to 10～20 ℃ subsequently.With UHT superhigh temperature instantaneously sterilising (140 ℃ of temperature, 30 seconds), and use aseptic cold filling technology packing.
Though the present invention discloses as above with preferred embodiment; Right its is not in order to limit the present invention; Any those skilled in the art; In not breaking away from spirit of the present invention and scope, when can doing a little change and modification, so protection scope of the present invention should be looked above the appending claims person of defining and is as the criterion.
Claims (22)
1. one kind prepares the method that is rich in galactooligosaccharide and low lactose milk products, it is characterized by with lactase (lactase) and directly handles dairy milk starting material.
2. the method for claim 1, wherein said dairy milk starting material is from mammal species.
3. method as claimed in claim 2, wherein said dairy milk starting material are ox, goat or sheep breast.
4. the method for claim 1, wherein said dairy milk starting material are to be dissolved in the water lactogenesis or milk powder or lactalbumin made.
5. the method for claim 1, wherein said dairy milk starting material is to be the high concentration state.
6. the method for claim 1; Wherein said lactase is beta galactosidase (β-galactosidase), or be the lactase from Aspergillus (Aspergillus), saccharomycete (Saccharomyces), Kluyveromyces sp (Kluyveromyces).
7. like the described method of arbitrary claim in the claim 1 to 6, wherein be to use the lactase that is about 0.1 to 0.5% (w/w).
8. like the described method of arbitrary claim in the claim 1 to 6, it further comprises the dairy milk starting material that uses Protease Treatment to handle through lactase.
9. method as claimed in claim 8, wherein said protease are flavor protease (flavourzyme), or from the fungal proteinase of aspergillus oryzae (Aspergillus oryzae).
10. method as claimed in claim 8 wherein is to use the protease that is about 0.1 to 0.5% (w/w).
11. like the described method of arbitrary claim in the claim 1 to 6, it is to about 60 ℃ temperature, to carry out the enzyme processing at about 30 ℃.
12. method as claimed in claim 8, it is to about 60 ℃ temperature, to carry out the enzyme processing at about 30 ℃.
13. like the described method of arbitrary claim in the claim 1 to 6, wherein after enzyme was finished dealing with, the enzyme that goes out of heating was lowered the temperature subsequently.
14. method as claimed in claim 8, wherein after enzyme was finished dealing with, the enzyme that goes out of heating was lowered the temperature subsequently.
15. method as claimed in claim 13, it is further with UHT superhigh temperature instantaneously sterilising.
16. method as claimed in claim 14, it is further with UHT superhigh temperature instantaneously sterilising.
17. method as claimed in claim 15, it further comprises the dairy products with aseptic cold filling technology packing gained.
18. method as claimed in claim 16, it further comprises the dairy products with aseptic cold filling technology packing gained.
19. dairy products that are rich in galactooligosaccharide, it is prepared by the described method of arbitrary claim in the claim 1 to 18.
20. milk product as claimed in claim 19, the solid content of the newborn product after wherein enzyme is handled is 8～60%w/w, contains the galactooligosaccharide of have an appointment 0.3～8% (w/w).
21. dairy products as claimed in claim 19, wherein the solid content of final liquid diary product is 7～28%w/w, contains the galactooligosaccharide of have an appointment 0.2～5% (w/w).
22. like the described dairy products of arbitrary claim in the claim 19 to 21; The solid content of the newborn product after wherein enzyme is handled is 40%w/w; Contain lactose and be lower than about 4% (w/w), and the solid content 12%w/w of final liquid diary product contains lactose and is lower than about 1.5% (w/w).
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| CN102726522A (en) * | 2012-04-26 | 2012-10-17 | 山东东兴生物科技股份有限公司 | Preparation method of low-lactose pasteurized milk |
| CN103976018A (en) * | 2013-02-08 | 2014-08-13 | 爱之味股份有限公司 | A method for preparing functional fermented dairy product and functional fermented dairy product prepared by the method |
| CN104186651A (en) * | 2014-07-31 | 2014-12-10 | 光明乳业股份有限公司 | Brown lactic acid bacteria drink and preparation method thereof |
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| CN108138208A (en) * | 2016-01-12 | 2018-06-08 | 维塔鲁斯营养有限公司 | By the method for lactose production galactooligosacchari(es |
| CN109463437A (en) * | 2018-12-27 | 2019-03-15 | 上海昊岳食品科技有限公司 | A method of shortening lactic acid bacteria lag phase in acidified milk production |
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| CN102726522A (en) * | 2012-04-26 | 2012-10-17 | 山东东兴生物科技股份有限公司 | Preparation method of low-lactose pasteurized milk |
| CN103976018A (en) * | 2013-02-08 | 2014-08-13 | 爱之味股份有限公司 | A method for preparing functional fermented dairy product and functional fermented dairy product prepared by the method |
| CN103976018B (en) * | 2013-02-08 | 2016-03-23 | 爱之味股份有限公司 | A method for preparing functional fermented dairy product and functional fermented dairy product prepared by the method |
| CN104186651A (en) * | 2014-07-31 | 2014-12-10 | 光明乳业股份有限公司 | Brown lactic acid bacteria drink and preparation method thereof |
| CN104186651B (en) * | 2014-07-31 | 2016-08-17 | 光明乳业股份有限公司 | A kind of brown lactic acid bacteria beverage and preparation method thereof |
| CN108138208A (en) * | 2016-01-12 | 2018-06-08 | 维塔鲁斯营养有限公司 | By the method for lactose production galactooligosacchari(es |
| CN106857819A (en) * | 2016-10-21 | 2017-06-20 | 帝斯曼知识产权资产管理有限公司 | Milk easy to digest and preparation method thereof |
| CN109548870A (en) * | 2017-09-25 | 2019-04-02 | 爱之味股份有限公司 | Oligosaccharide-rich milk product with functions of regulating blood fat, improving intestinal flora and enhancing immunity and preparation method thereof |
| US11241018B2 (en) | 2017-09-25 | 2022-02-08 | A.G.V. Products Corp. | Process for preparing oligo-saccharide enhanced milk products |
| CN109463437A (en) * | 2018-12-27 | 2019-03-15 | 上海昊岳食品科技有限公司 | A method of shortening lactic acid bacteria lag phase in acidified milk production |
| CN114258950A (en) * | 2021-12-28 | 2022-04-01 | 光明乳业股份有限公司 | Yoghourt containing galacto-oligosaccharide and preparation method thereof |
| CN114271328A (en) * | 2021-12-28 | 2022-04-05 | 光明乳业股份有限公司 | Lactobacillus acidophilus milk rich in rhamnose and preparation method thereof |
| CN114271327A (en) * | 2021-12-28 | 2022-04-05 | 光明乳业股份有限公司 | Zero-lactose fermented milk and preparation method thereof |
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