CN102362895A - Method for extracting medicinal ingredient against methicillin-resistant staphylococcus aureus from peony seeds - Google Patents
Method for extracting medicinal ingredient against methicillin-resistant staphylococcus aureus from peony seeds Download PDFInfo
- Publication number
- CN102362895A CN102362895A CN2011103683124A CN201110368312A CN102362895A CN 102362895 A CN102362895 A CN 102362895A CN 2011103683124 A CN2011103683124 A CN 2011103683124A CN 201110368312 A CN201110368312 A CN 201110368312A CN 102362895 A CN102362895 A CN 102362895A
- Authority
- CN
- China
- Prior art keywords
- extract
- obtains
- staphylococcus aureus
- subsequent use
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a method for extracting a medicinal ingredient against methicillin-resistant staphylococcus aureus from peony seeds, which comprises: mixing peony seeds and ethanol solution, and soaking the peony seeds under a closed condition for 2 to 5 days; obtaining extract I and filter residue I; soaking the filter residue I in ethanol again to obtain extract II; mixing the extract I and the extract II, adding water into the mixed extract, mixing, standing and obtaining supernate; subjecting the obtained supernate to extraction by an ethyl acetate solvent twice; mixing the extract obtained by the two times of extraction; and adding saturated solution of n-butyl alcohol into the mixture for extraction twice, mixing the n-butyl alcohol extract obtained by the two times of extraction, standing the mixture for 2 to 5 days, and obtaining the product. The method for extracting the medicinal ingredient against methicillin-resistant staphylococcus aureus from peony seeds, which is provided by the invention, is simple in process and operation, the content of the medicinal ingredient against methicillin-resistant staphylococcus aureus, which is obtained from peony seeds, is high, the purity of the medicinal ingredient is high, and the antibacterial effect is obvious.
Description
Technical field
The present invention relates to a kind of method for distilling of pharmaceutical compositions, seed of Flos Moutan extracts the method for methicillin-resistant staphylococcus aureus resistance ingredient specifically.
Background technology
Along with antibiotic extensive use, the variation of bacterial structure, form cause increasing bacterial antibiotic to produce drug resistance, and drug-resistant intensity is strengthened day by day.At present methicillin-resistant staphylococcus aureus (MRSA) infection, hepatitis b virus hbv and AIDS are listed as three big scabrous infectious diseases in the world wide.Methicillin-resistant staphylococcus aureus all has drug resistance to multiple antibiotic, and the pathogenicity of methicillin-resistant staphylococcus aureus is strong and therapeutic effect is poor, is to cause one of The main pathogenic fungi that hospital clinical infects.Though the available vancomycin treatment of infection that clinically methicillin-resistant staphylococcus aureus is caused; In recent years; Vancomycin is regarded as in the increasing hospital after the conventional antibiotics medication, and also engenders drug resistance, at present; For the drug resistance growth rate of slowing down methicillin-resistant staphylococcus aureus; Remove through the restriction abuse of antibiotics and slow down drug resistance MRSA growth rate, the medicine of simultaneously must the research and development new caused disease of methicillin-resistant staphylococcus aureus resistance in support, in case the great outburst of methicillin-resistant staphylococcus aureus disease occurs.Therefore the antibiotic that screens and develop effectively methicillin-resistant staphylococcus aureus resistance infection is extremely urgent.
Seed of Flos Moutan is usually used in treating lumbago and skelalgia among the people, has antioxidation, anti-inflammatory analgetic effect, and seed of Flos Moutan is as potential medicine resource, remains deep development and use.Seed of Flos Moutan is used for breeding more at present, refining Paeonia suffruticosa oil, and its medical value is not obtained exploitation as yet.Find to show according to early-stage Study, mainly contain oil substances such as linolenic acid, oleic acid, linoleic acid, Palmic acid and stearic acid in the peony seed oil; Relevant non-greasy chemical constituent has oleanolic acid, helexin, kaempferol; Luteolin, apigenin, chrysoeriol; Materials such as stigmasterol, known in addition seed of Flos Moutan be to bacillus subtilis, Salmonella; Rhizopus and Aspergillus niger and Ba Men Salmonella have certain bacteriostasis, and the activity that methicillin-resistant staphylococcus aureus resistance infects does not still have relevant report.
Summary of the invention
The present invention is directed to the deficiency of the problems referred to above; The method of from seed of Flos Moutan, extracting the methicillin-resistant staphylococcus aureus resistance ingredient is provided; Its technology is simple, and is easy to operate, and the methicillin-resistant staphylococcus aureus resistance content of active component that from seed of Flos Moutan, obtains is high; Purity is high, and antibacterial effect is remarkable.
The present invention addresses the above problem the technical scheme that is adopted to be: from seed of Flos Moutan, extract the method for methicillin-resistant staphylococcus aureus resistance ingredient, its concrete steps are:
Step 2, in the container that contains the filtering residue I, to add 200-300 parts of concentration be 60-80% alcoholic solution, and mix homogeneously carries out airtight immersion 2-3 days; Filter, obtain extracting solution II and filtering residue II, extracting solution I that obtains and extracting solution II are mixed; Obtain mixed liquor, subsequent use;
Step 3, mark is than getting 1 part mixed liquor and 8-16 parts water by weight, and mix homogeneously left standstill 2-5 hours, filtered and obtained supernatant, and is subsequent use;
Step 4, the mark ratio is got 1 part of supernatant and 1 part of ethyl acetate solvent by weight, puts into separatory funnel, extracts, and obtains the extract I on upper strata and the separating medium I of lower floor, and is subsequent use;
Step 5, the mark ratio is got 1 part of separating medium I and 1 part of ethyl acetate solvent by weight, puts into separatory funnel, extracts, and obtains the extract II, with extract I and extract II mix homogeneously, leaves standstill, and obtains acetic acid ethyl ester extract, and is subsequent use;
Step 6, the mark ratio is got 1 part of acetic acid ethyl ester extract and 1 part of saturated butanol solution by weight, puts into separatory funnel, extracts, and obtains the extract III on upper strata and the separating medium II of lower floor, and is subsequent use;
Step 7, the mark ratio is got 1 part of separating medium II and 1 saturated part of butanol solution by weight, puts into separatory funnel, extracts, and obtains the extract IV, and with extract III and extract IV mix homogeneously, the evaporative removal solvent obtains product.
Beneficial effect
1, in the present invention, find a kind of novel anti methicillin-resistant staphylococcus aureus pharmaceutical compositions.
2, in the present invention, through seed of Flos Moutan is extracted, the methicillin-resistant staphylococcus aureus resistance pharmaceutical compositions extract that obtains; Its productive rate is greater than 65%; Through detecting, have the effect that methicillin-resistant staphylococcus aureus resistance infects, improved the medical value of Paeonia suffruticosa.
3, improve economic benefit and the medical value of Paeonia suffruticosa, a new way is opened up in the comprehensive utilization of Paeonia suffruticosa and the deep processing of Paeonia suffruticosa industry.
4, simple, the safety, reliable of operating procedure of the present invention can be applied in the large-scale production fully.
Description of drawings
Fig. 1 is 12 hours susceptibility observation figure;
Fig. 2 is 32 hours susceptibility observation figure;
Label among the figure: 10,000 for concentration be the vancomycin susceptibility sheet of 5mg/mL; 10,000 for concentration be the vancomycin susceptibility sheet of 10mg/mL; Green grass or young crops 1 is the penicillin susceptibility sheet of 5mg/mL for concentration; Blue or green 10 for concentration is the penicillin susceptibility sheet of 10mg/mL, and positive 15 for concentration is the susceptibility sheet of the methicillin-resistant staphylococcus aureus resistance pharmaceutical compositions extract of 5mg/mL, positive 15 for concentration be the susceptibility sheet of the methicillin-resistant staphylococcus aureus resistance pharmaceutical compositions of 10mg/mL.
The specific embodiment
Seed of Flos Moutan extracts the method for methicillin-resistant staphylococcus aureus resistance ingredient, and its concrete steps are:
Step 2, in the container that contains the filtering residue I, to add 200-300 parts of concentration be 60-80% alcoholic solution, and mix homogeneously carries out airtight immersion 2-3 days; Filter, obtain extracting solution II and filtering residue II, extracting solution I that obtains and extracting solution II are mixed; Obtain mixed liquor, subsequent use;
Step 3, mark is than getting 1 part mixed liquor and 8-16 parts water by weight, and mix homogeneously left standstill 2-5 hours, filtered and obtained supernatant, and is subsequent use;
Step 4, the mark ratio is got 1 part of supernatant and 1 part of ethyl acetate solvent by weight, puts into separatory funnel, extracts, and obtains the extract I on upper strata and the separating medium I of lower floor, and is subsequent use;
Step 5, the mark ratio is got 1 part of separating medium I and 1 part of ethyl acetate solvent by weight, puts into separatory funnel, extracts, and obtains the extract II, with extract I and extract II mix homogeneously, leaves standstill, and obtains acetic acid ethyl ester extract, and is subsequent use;
Step 6, the mark ratio is got 1 part of acetic acid ethyl ester extract and 1 part of saturated butanol solution by weight, puts into separatory funnel, extracts, and obtains the extract III on upper strata and the separating medium II of lower floor, and is subsequent use;
Step 7, the mark ratio is got 1 part of separating medium II and 1 part of saturated butanol solution by weight, puts into separatory funnel, extracts, and obtains the extract IV, and with extract III and extract IV mix homogeneously, the evaporative removal solvent obtains product.
Embodiment one
Seed of Flos Moutan extracts the method for methicillin-resistant staphylococcus aureus resistance ingredient, and its concrete steps are:
Step 2, in the container that contains the filtering residue I, to add 200 parts of concentration be 60% alcoholic solution, and mix homogeneously carries out airtight immersion 2 days; Filter, obtain extracting solution II and filtering residue II, extracting solution I that obtains and extracting solution II are mixed; Obtain mixed liquor, subsequent use;
Step 3, mark is than getting 1 part mixed liquor and 8 parts water by weight, and mix homogeneously left standstill 2 hours, filtered and obtained supernatant, and is subsequent use;
Step 4, the mark ratio is got 1 part of supernatant and 1 part of ethyl acetate solvent by weight, puts into separatory funnel, extracts, and obtains the extract I on upper strata and the separating medium I of lower floor, and is subsequent use;
Step 5, the mark ratio is got 1 part of separating medium I and 1 part of ethyl acetate solvent by weight, puts into separatory funnel, extracts, and obtains the extract II, with extract I and extract II mix homogeneously, leaves standstill, and obtains acetic acid ethyl ester extract, and is subsequent use;
Step 6, the mark ratio is got 1 part of acetic acid ethyl ester extract and 1 part of saturated butanol solution by weight, puts into separatory funnel, extracts, and obtains the extract III on upper strata and the separating medium II of lower floor, and is subsequent use;
Step 7, the mark ratio is got 1 part of separating medium II and 1 part of saturated butanol solution by weight, puts into separatory funnel, extracts; Obtain the extract IV; With extract III and extract IV mix homogeneously, the evaporative removal solvent gets yellow extractum; Be the extract that obtains the methicillin-resistant staphylococcus aureus resistance pharmaceutical compositions, the productive rate of its extract is 65%.
Embodiment two
Seed of Flos Moutan extracts the method for methicillin-resistant staphylococcus aureus resistance ingredient, and its concrete steps are:
Step 2, in the container that contains the filtering residue I, to add 250 parts of concentration be 70% alcoholic solution, and mix homogeneously carries out airtight immersion 3 days; Filter, obtain extracting solution II and filtering residue II, extracting solution I that obtains and extracting solution II are mixed; Obtain mixed liquor, subsequent use;
Step 3, mark is than getting 1 part mixed liquor and 12 parts water by weight, and mix homogeneously left standstill 3 hours, filtered and obtained supernatant, and is subsequent use;
Step 4, the mark ratio is got 1 part of supernatant and 1 part of ethyl acetate solvent by weight, puts into separatory funnel, extracts, and obtains the extract I on upper strata and the separating medium I of lower floor, and is subsequent use;
Step 5, the mark ratio is got 1 part of separating medium I and 1 part of ethyl acetate solvent by weight, puts into separatory funnel, extracts, and obtains the extract II, with extract I and extract II mix homogeneously, leaves standstill, and obtains acetic acid ethyl ester extract, and is subsequent use;
Step 6, the mark ratio is got 1 part of acetic acid ethyl ester extract and 1 part of saturated butanol solution by weight, puts into separatory funnel, extracts, and obtains the extract III on upper strata and the separating medium II of lower floor, and is subsequent use;
Step 7, the mark ratio is got 1 part of separating medium II and 1 part of saturated butanol solution by weight, puts into separatory funnel, extracts; Obtain the extract IV; With extract III and extract IV mix homogeneously, the evaporative removal solvent obtains yellow extractum; Promptly obtain the extract of methicillin-resistant staphylococcus aureus resistance pharmaceutical compositions, the productive rate of its extract is 67%.
Embodiment three
Seed of Flos Moutan extracts the method for methicillin-resistant staphylococcus aureus resistance ingredient, and its concrete steps are:
Step 2, in the container that contains the filtering residue I, to add 300 parts of concentration be 80% alcoholic solution, and mix homogeneously carries out airtight immersion 3 days; Filter, obtain extracting solution II and filtering residue II, extracting solution I that obtains and extracting solution II are mixed; Obtain mixed liquor, subsequent use;
Step 3, mark is than getting 1 part mixed liquor and 16 parts water by weight, and mix homogeneously left standstill 5 hours, filtered and obtained supernatant, and is subsequent use;
Step 4, the mark ratio is got 1 part of supernatant and 1 part of ethyl acetate solvent by weight, puts into separatory funnel, extracts, and obtains the extract I on upper strata and the separating medium I of lower floor, and is subsequent use;
Step 5, the mark ratio is got 1 part of separating medium I and 1 part of ethyl acetate solvent by weight, puts into separatory funnel, extracts, and obtains the extract II, with extract I and extract II mix homogeneously, leaves standstill, and obtains acetic acid ethyl ester extract, and is subsequent use;
Step 6, the mark ratio is got 1 part of acetic acid ethyl ester extract and 1 part of saturated butanol solution by weight, puts into separatory funnel, extracts, and obtains the extract III on upper strata and the separating medium II of lower floor, and is subsequent use;
Step 7, the mark ratio is got 1 part of separating medium II and 1 part of saturated butanol solution by weight, puts into separatory funnel, extracts; Obtain the extract IV; With extract III and extract IV mix homogeneously, the evaporative removal solvent obtains yellow extractum; Promptly obtain the extract of methicillin-resistant staphylococcus aureus resistance pharmaceutical compositions, the productive rate of its extract is 70%.
Drug sensitive test:
The preparation of susceptibility sheet: in the penicillin solution that concentration is the extract solution of the extract solution of the methicillin-resistant staphylococcus aureus resistance pharmaceutical compositions of 5mg/mL, methicillin-resistant staphylococcus aureus resistance pharmaceutical compositions that concentration is 10mg/mL, vancomycin solution that concentration is 5mg/mL, concentration is 10mg/mL vancomycin solution, penicillin solution that concentration is 5mg/mL and concentration are 10mg/mL, put into the drug sensitive test paper that diameter is 6mm; Soaked 24 hours; Make concentration and be the penicillin susceptibility sheet that the susceptibility sheet of the susceptibility sheet of the methicillin-resistant staphylococcus aureus resistance pharmaceutical compositions extract of 5mg/mL, methicillin-resistant staphylococcus aureus resistance pharmaceutical compositions that concentration is 10mg/mL, vancomycin susceptibility sheet that concentration is 5mg/mL, vancomycin susceptibility sheet that concentration is 10mg/mL, penicillin susceptibility sheet that concentration is 5mg/mL and concentration are 10mg/mL, subsequent use;
The activation of methicillin-resistant staphylococcus aureus: in aseptic processing room, the mark ratio is got 1 part and is contained 3.2 * 10 by weight
8The methicillin-resistant staphylococcus aureus suspension of cfu/mL and 19 parts of agarose culture medium; Mix homogeneously, the culture medium that will contain methicillin-resistant staphylococcus aureus is poured in the culture dish, and the thickness of culture medium is 4-5mm; The culture dish that will contain culture medium was placed 30 minutes, and is subsequent use;
Bacteriostatic experiment: the penicillin susceptibility sheet that the concentration for preparing is the susceptibility sheet of the susceptibility sheet of the methicillin-resistant staphylococcus aureus resistance pharmaceutical compositions extract of 5mg/mL, methicillin-resistant staphylococcus aureus resistance pharmaceutical compositions that concentration is 10mg/mL, vancomycin susceptibility sheet that concentration is 5mg/mL, concentration is 10mg/mL vancomycin susceptibility sheet, penicillin susceptibility sheet that concentration is 5mg/mL and concentration are 10mg/mL is placed on respectively in the culture medium that contains methicillin-resistant staphylococcus aureus; After cultivating 15 hours under 37 ℃ of conditions, observe the inhibition zone in the culture medium;
The result finds, after cultivating 12 hours, observes, and concentration is that the inhibition zone of the methicillin-resistant staphylococcus aureus resistance pharmaceutical compositions extract susceptibility sheet of 5mg/mL is 10mm; Concentration is that the inhibition zone of the methicillin-resistant staphylococcus aureus resistance pharmaceutical compositions susceptibility sheet of 10mg/mL is 15mm; Concentration is that the inhibition zone of the vancomycin susceptibility sheet of 5mg/mL is 15mm; Concentration is that the inhibition zone of the vancomycin susceptibility sheet of 10mg/mL is 20mm; Concentration is the inhibition zone that the penicillin susceptibility sheet of 5mg/mL does not have, and concentration is the penicillin susceptibility sheet of 10mg/mL fuzzy inhibition zone is arranged; And after cultivating 32 hours, observe; It in concentration the bacterium colony that has single methicillin-resistant staphylococcus aureus on the inhibition zone of vancomycin susceptibility sheet of 10mg/mL; Show that vancomycin has drug resistance, and methicillin-resistant staphylococcus aureus resistance pharmaceutical compositions extract, have the effect of significant methicillin-resistant staphylococcus aureus resistance; And fungistatic effect is stable, does not have drug resistance.
Claims (1)
1. seed of Flos Moutan extracts the method for methicillin-resistant staphylococcus aureus resistance ingredient, and it is characterized in that: concrete steps are:
Step 1, by weight the mark ratio to get 100 parts of seed of Flos Moutan and 300-400 parts of concentration be 60-80% alcoholic solution, earlier seed of Flos Moutan is crushed to 200-400 purpose granules, in the seed of Flos Moutan granule, add alcoholic solution; Mix homogeneously; Put into container, carry out airtight immersion 2-5 days, filter; Obtain extracting solution I and filtering residue I, subsequent use;
Step 2, in the container that contains the filtering residue I, to add 200-300 parts of concentration be 60-80% alcoholic solution, and mix homogeneously carries out airtight immersion 2-3 days; Filter, obtain extracting solution II and filtering residue II, extracting solution I that obtains and extracting solution II are mixed; Obtain mixed liquor, subsequent use;
Step 3, mark is than getting 1 part mixed liquor and 8-16 parts water by weight, and mix homogeneously left standstill 2-5 hours, filtered and obtained supernatant, and is subsequent use;
Step 4, the mark ratio is got 1 part of supernatant and 1 part of ethyl acetate solvent by weight, puts into separatory funnel, extracts, and obtains the extract I on upper strata and the separating medium I of lower floor, and is subsequent use;
Step 5, the mark ratio is got 1 part of separating medium I and 1 part of ethyl acetate solvent by weight, puts into separatory funnel, extracts, and obtains the extract II, with extract I and extract II mix homogeneously, leaves standstill, and obtains acetic acid ethyl ester extract, and is subsequent use;
Step 6, the mark ratio is got 1 part of acetic acid ethyl ester extract and 1 part of saturated butanol solution by weight, puts into separatory funnel, extracts, and obtains the extract III on upper strata and the separating medium II of lower floor, and is subsequent use;
Step 7, the mark ratio is got 1 part of separating medium II and 1 saturated part of butanol solution by weight, puts into separatory funnel, extracts, and obtains the extract IV, and with extract III and extract IV mix homogeneously, the evaporative removal solvent obtains product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110368312 CN102362895B (en) | 2011-11-19 | 2011-11-19 | Method for extracting medicinal ingredient against methicillin-resistant staphylococcus aureus from peony seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110368312 CN102362895B (en) | 2011-11-19 | 2011-11-19 | Method for extracting medicinal ingredient against methicillin-resistant staphylococcus aureus from peony seeds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102362895A true CN102362895A (en) | 2012-02-29 |
| CN102362895B CN102362895B (en) | 2013-07-17 |
Family
ID=45689511
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110368312 Active CN102362895B (en) | 2011-11-19 | 2011-11-19 | Method for extracting medicinal ingredient against methicillin-resistant staphylococcus aureus from peony seeds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102362895B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103272008A (en) * | 2013-06-11 | 2013-09-04 | 洛阳建洛生物科技有限公司 | Technology for synergistically extracting peony flavone |
| CN106265977A (en) * | 2015-06-04 | 2017-01-04 | 伽蓝（集团）股份有限公司 | Seed of Flos Moutan extract and preparation method and application, include its skin preparations for extenal use |
| CN107698553A (en) * | 2017-09-27 | 2018-02-16 | 山东省科学院生物研究所 | Chlorine-containing compound and preparation method and purposes |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1642564A (en) * | 2002-01-26 | 2005-07-20 | Mst有限公司 | Composition containing moutan root bark extract as active ingredient |
| CN101647872A (en) * | 2009-09-07 | 2010-02-17 | 兰州牡丹园艺开发公司 | Peony essential oil soft capsule and preparation method thereof |
-
2011
- 2011-11-19 CN CN 201110368312 patent/CN102362895B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1642564A (en) * | 2002-01-26 | 2005-07-20 | Mst有限公司 | Composition containing moutan root bark extract as active ingredient |
| CN101647872A (en) * | 2009-09-07 | 2010-02-17 | 兰州牡丹园艺开发公司 | Peony essential oil soft capsule and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 白喜婷: "牡丹籽提取物的抑菌特性研究", 《中国酿造》, no. 3, 31 December 2009 (2009-12-31), pages 59 - 61 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103272008A (en) * | 2013-06-11 | 2013-09-04 | 洛阳建洛生物科技有限公司 | Technology for synergistically extracting peony flavone |
| CN106265977A (en) * | 2015-06-04 | 2017-01-04 | 伽蓝（集团）股份有限公司 | Seed of Flos Moutan extract and preparation method and application, include its skin preparations for extenal use |
| CN106265977B (en) * | 2015-06-04 | 2021-07-20 | 伽蓝（集团）股份有限公司 | Peony seed extract, preparation method and application thereof, and skin external preparation comprising peony seed extract |
| CN107698553A (en) * | 2017-09-27 | 2018-02-16 | 山东省科学院生物研究所 | Chlorine-containing compound and preparation method and purposes |
| CN107698553B (en) * | 2017-09-27 | 2019-08-09 | 山东省科学院生物研究所 | Chlorine-containing compound and preparation method and purposes |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102362895B (en) | 2013-07-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5269160B2 (en) | Bioactive liquid composition and method for producing the same | |
| CN104593425B (en) | A kind of stem of noble dendrobium red yeast rice preparation method | |
| CN102362895B (en) | Method for extracting medicinal ingredient against methicillin-resistant staphylococcus aureus from peony seeds | |
| CN102731365A (en) | Hericium erinaceum biological micro-molecules for inhibiting helicobacter pylori and use of the hericium erinaceum biological micro-molecules in treatment of digestive tract diseases | |
| CN102690858A (en) | Preparation method for grape seed proanthocyanidin extract | |
| CN102038014A (en) | Compound plant-source acaricide and preparation method thereof | |
| CN106729520A (en) | A kind of preparation method of Ginger P.E | |
| CN103222486B (en) | Plant extract with inhibiting effect on orange pathogenic bacteria as well as preparation method and application thereof | |
| CN102577951B (en) | Method for producing huperzine A by using huperzia serrata prothallium | |
| CN104845943B (en) | A kind of streptococcus phage and its application | |
| TW200942252A (en) | Anti-methicillin-resistant staphylococcus aureus agent and anti-vancomycin-resistant enterococcus agent | |
| CN104031109A (en) | Method for purifying tea saponin by microbial fermentation | |
| CN103103127A (en) | Culture method for microalgae | |
| CN104059869A (en) | Marine Bacillus amyloliquefaciens and culturing method and application thereof | |
| CN102772447A (en) | Method for extracting bacteriostatic agent from aschersoniaaleyrodis metabolin | |
| CN104758340B (en) | Coffee mesitoyl quinine acid extract and its preparation method and application in a kind of folium lonicerae | |
| CN105232613B (en) | Extract of caragana plant bark ethyl acetate, extraction method and application | |
| CN103520323B (en) | Application of Nauclea officinalis extract to preparation of medicament for resisting methicillin-resistant Staphylococcus aureus | |
| CN104873510A (en) | Use of oxazolidinone compound for resisting biofilm | |
| CN113082104B (en) | Composite antibacterial containing pomegranate peel water extract and pepper seed water extract | |
| CN109851500B (en) | Perilla oxygen carbonyl menthyl propionate and application thereof | |
| CN113813303B (en) | Application of cyclocarya paliurus leaf extract in preparation of antibacterial drug and/or antibacterial agent for inhibiting drug-resistant bacteria | |
| CN108041038A (en) | Citrus processing generates application of the small molecule as copper agent bactericide synergist in disease control | |
| CN107396938A (en) | A kind of soil insecticide and preparation method thereof | |
| CN106305796A (en) | Natural sterilization insecticide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |