CN102357142B - Use of extract of Chinese herbal medicine seville orange flower in preparation of drug for losing weight and reducing blood fat or drug for inhibiting lipase activity - Google Patents
Use of extract of Chinese herbal medicine seville orange flower in preparation of drug for losing weight and reducing blood fat or drug for inhibiting lipase activity Download PDFInfo
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Abstract
The invention discloses a use of water extract or ethanol extract of Chinese herbal medicine seville orange flower in preparation of a drug for losing weight and reducing blood fat, or a drug for inhibiting lipase activity. The water extract or ethanol extract of Chinese herbal medicine seville orange flower overcomes the defect that the existing clinical drug for losing weight has a low traditional Chinese medicine effective rate and large side effects. The water extract or ethanol extract of Chinese herbal medicine seville orange flower is a drug which is utilized for losing weight and reducing blood fat and has the advantages of clear mechanism, scientific target, small side-effect and high clinical effective rate.
Description
Technical field
The present invention relates to the application of Chinese herbal medicine CitrusaurantiumL.Var.amara Engl. extract in preparing Weight-lossing hypolipemic medicine or preparation and have the medicine of lipase activity inhibition.
Background technology
Metabolism syndrome (Metabolic syndrome, MS) mainly comprises the risk factors such as obesity, impaired glucose tolerance, metabolism disorder of blood lipid, hypertension.The sickness rate of MS patient's diabetes (Diabetes mellitus, DM), cardiovascular disease is often the several times of normal population.MS is the serious harm mankind's physical and mental health, the public health problem that becomes international, but we are very limited to the understanding of MS so far.Preliminary studies confirm that at present, in numerous causes of disease, fat particularly close with the relation of MS, be key factor and the core link of MS morbidity, development.Along with the raising of expanding economy, people's living standard and the change of life style, the fat chronic disease that has become global adult's maximum.
At present, U.S. FDA only ratified 2 kinds of medicines can be for a long time for bariatrician: sibutramine (Sibutramine) and orlistat (Orlistat).Sibutramine is a kind of monoamine neurotransmitter reuptake inhibitor, it acts on central nervous system and with minimizing, ingests and reduce body weight, but this medicine may cause patient's hypertension, heart rate and accelerate, and can not, for the patient of the medical histories such as coronary heart disease, arrhythmia and apoplexy, this medicine application be restricted.Orlistat is a kind of pancreatic lipase inhibitor, can suppress the activity of pancreatic lipase in gastrointestinal tract, and makes fat absorption reduce approximately 30%, and its systemic side effects is starkly lower than other existing appetrol, is a successful example of lipase inhibitor.
Chinese herbal medicine is the natural pharmaceutical resources of the unique advantage of China, and classic of TCM record at all times and clinical practice have the example of a large amount of Chinese medicinal herb weight-losing blood fat reducings.But because Chinese herbal medicine active component is indefinite, the restriction of the unclear grade of pharmacological mechanism, cannot go to the world Chinese traditional herbs, and China does not also have the slimming medicine of independent intellectual property right so far.Therefore from Chinese herbal medicine, screening has the effective ingredient of lipase activity inhibition, and carries out the development of Weight-lossing hypolipemic medicine, is main purpose of the present invention.
Summary of the invention
The object of the invention is to the application of open Chinese herbal medicine CitrusaurantiumL.Var.amara Engl. extract in preparing Weight-lossing hypolipemic medicine or preparation and have the medicine of lipase activity inhibition.
The present invention seeks to be achieved through the following technical solutions:
The water extract of following Chinese herbal medicine or alcohol extract all have lipase activity inhibition:
Radix Scutellariae, Folium camelliae assamicae, oolong tea, TIEGUANYIN tea, Folium Ilicis, black tea, green tea, CitrusaurantiumL.Var.amara Engl., Flos Carthami, Pericarpium Zanthoxyli, Aloe, Rhizoma Polygoni Cuspidati, Folium Sennae, Radix Puerariae, Radix Salviae Miltiorrhizae, the Rhizoma Atractylodis Macrocephalae, Flos persicae, Herba Apocyni veneti, Flos Chrysanthemi, Flos Campsis, Flos Jasmini Sambac, Semen Oroxyli, Semen Vitis viniferae, Flos Trollii, Flos Caryophylli, Flos Lonicerae, Radix Cynanchi Atrati, Radix Sophorae Flavescentis, Herba Cassiae Nomamis, Folium Artemisiae Argyi, Radix Ginseng, the Flos Sophorae Immaturus, Rhizoma Fagopyri Dibotryis, Rhizoma Alismatis, Fructus Aurantii, Fructus Aurantii Immaturus, Radix Achyranthis Bidentatae, Fructus Chebulae, Caulis Polygoni Multiflori, the Radix Aucklandiae, Folium Ginkgo, Rhizoma Osmundae, the Cortex Eucommiae, Pollen Tyjphae, Fructus Trichosanthis, Folium Mori, Cortex Mori,
The preparation method of said herbal medicine water extract is as follows: get above-mentioned each single herbal decoction piece, and prefabricated, pulverize; Add 5-10 volume deionized water doubly, boil 30-60 minute, filtration or centrifugal, gets filtrate or supernatant and obtains extract; The preparation method of said herbal medicine alcohol extract is as follows: get above-mentioned each single herbal decoction piece, and prefabricated, pulverize; Add 5-10 volume 50%-95% ethanol doubly, supersound extraction 30-90 minute, filtration or centrifugal, gets filtrate or supernatant and obtains extract, gets supernatant and obtains extract;
The preferred preparation method of said herbal medicine water extract is as follows: get above-mentioned each single herbal decoction piece, and prefabricated, pulverize; Add 7 volumes deionized water doubly, boil 45 minutes, filtration or centrifugal, gets filtrate or supernatant and obtains extract; The preferred preparation method of said herbal medicine alcohol extract is as follows: get above-mentioned each single herbal decoction piece, and prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes, filtration or centrifugal, gets filtrate or supernatant and obtains extract.
The alcohol extract of following Chinese herbal medicine has lipase activity inhibition:
Radix Et Rhizoma Rhei, Radix Glycyrrhizae, Rhizoma Chuanxiong, Fructus Chaenomelis, Caulis Spatholobi, Cortex Magnoliae Officinalis, Cortex Moutan, Herb Gynostemmae Pentaphylli, Rhizoma Curcumae Longae, Rhizoma Et Radix Notopterygii, Radix Polygoni Multiflori, Semen Arecae, Ramulus Cinnamomi;
The preparation method of said herbal medicine alcohol extract is as follows: get above-mentioned each single herbal decoction piece, and prefabricated, pulverize; Add 5-10 volume 50%-95% ethanol doubly, supersound extraction 30-90 minute, filtration or centrifugal, gets filtrate or supernatant and obtains extract;
The preferred preparation method of said herbal medicine alcohol extract is as follows: get above-mentioned each single herbal decoction piece, and prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes, filtration or centrifugal, gets filtrate or supernatant and obtains extract.
The water extract of following Chinese herbal medicine has lipase activity inhibition:
Fructus Lycii, Folium Nelumbinis, Radix Platycodonis;
The preferred preparation method of said herbal medicine water extract is as follows: get above-mentioned each single herbal decoction piece, and prefabricated, pulverize; Add 5-10 volume deionized water doubly, boil 30-60 minute, filtration or centrifugal, gets filtrate or supernatant and obtains extract;
The preferred preparation method of said herbal medicine water extract is as follows: get above-mentioned each single herbal decoction piece, and prefabricated, pulverize; Add 7 volumes deionized water doubly, boil 45 minutes, filtration or centrifugal, gets filtrate or supernatant and obtains extract.
Said herbal medicine extract of the present invention all can be used by single in the application of preparing Weight-lossing hypolipemic medicine, also use capable of being combined, and Weight-lossing hypolipemic medicine can be made by water, alcohol and the extractive with organic solvent of said herbal medicine or the effective ingredient of other method purification;
Weight-lossing hypolipemic medicine can also comprise the active components playing weight losing and hypolipemic role of following one or more other mechanisms of action: glycosidase inhibitor, fatty acid synthetase inhibitor, cholesterol biosynthesis enzyme inhibitor, amylase inhibitor;
In order to increase drug effect or to reduce side effect, Weight-lossing hypolipemic medicine can also comprise the Chinese herbal medicine of following one or more other pharmacological actions: Fructus Corni, Herba Ephedrae, Folium Isatidis, Herba Taraxaci, Radix Rehmanniae Preparata;
Weight-lossing hypolipemic medicine can also comprise following one or more excipient: Radix Acaciae senegalis, skim milk, mannan, glucomannan, Rhizoma amorphophalli powder, starch, lipotropy sclerosing agent;
Weight-lossing hypolipemic medicine can also comprise following one or more pharmaceutic adjuvants or reduce the existing product of drug side effect: Radix Acaciae senegalis, skim milk, mannan, glucomannan, Rhizoma amorphophalli powder, starch, lipotropy sclerosing agent, stearic acid.
Above-mentioned lipase refers in particular to pancreatic lipase.
The present invention be take pancreatic lipase activity as target spot, the repressed degree of the pancreatic lipase activity of take is index, from a large amount of Chinese herbal medicine, screen said herbal medicine, its extract all has the effect of obvious inhibition lipase active, the invention solves existing clinical slimming medicine (especially Chinese medicine) effective percentage low, the shortcoming that side effect is large, provide that a kind of mechanism is clear and definite, target spot science, the Weight-lossing hypolipemic medicine that side effect is little, clinical effective rate is high.
Experimental example and embodiment are used for further illustrating but are not limited to the present invention below.
The water extract of experimental example 1 Chinese herbal medicine of the present invention and alcohol extract are tested lipase inhibitory action
1, water extract or the alcohol extract of preparation Chinese herbal medicine of the present invention:
Water extract preparation: get each single herbal decoction piece, prefabricated, pulverize; Add 5 volumes deionized water doubly, boil 30 minutes, filtration or centrifugal, gets filtrate or supernatant and obtains extract;
Ethanol extraction preparation: get each single herbal decoction piece, prefabricated, pulverize; Add 5 volumes, 75% ethanol doubly, supersound extraction 60 minutes, filtration or centrifugal, gets filtrate or supernatant and obtains extract.
2, the mensuration of lipase activity inhibition rate:
A. prepare reagent, olive oil emulsion and measure enzyme liquid:
The preparation of olive oil emulsion: get olive oil 12ml and Radix Acaciae senegalis 22.5g, grind evenly, add water and be ground to 300ml, the homogenizer emulsifying of use 18000r/min 2 times, each 3 minutes; Get Emulsion and check under the microscope, the diameter of 90% breast grain, below 3 μ m, and must not have the breast grain of 10 μ m;
The preparation of bovine bile solution: precision takes bovine bile 8.0g, is placed in 50ml beaker, with water dissolution, is transferred to standardize solution in 100ml volumetric flask;
The preparation of 0.1mol/L sodium hydroxide solution: accurate weighing sodium hydroxide 2.0g, is dissolved in water and is settled to 500ml;
TRIS buffer preparation: get Tris 3.030g, with 0.1mol/L hydrochloric acid solution adjust pH to 8.0, add water to 500ml, shake up, then regulate pH value to 8.0;
The preparation of lipase solution: precision takes pancreatic lipase (SIGMA:L3126) in volumetric flask, with being cooled to 5 ℃ of following TRIS buffer, dissolve, make the solution that contains pancreatic lipase 100 unit of activity in every 1ml, standby as proenzyme liquid; During drawing standard curve, proenzyme liquid is diluted to the mensuration enzyme liquid of different gradient 90u/ml, 80u/ml, 70u/ml, 60u/ml, 50u/ml, 40u/ml, 30u/ml, 20u/ml, 10u/ml by TRIS buffer; The mensuration enzyme liquid of 0u/ml, that is: enzyme is blank, is the proenzyme liquid boiling in water-bath 15-30 minute;
B. set up lipase and survey live body system:
1). water is that sample blank solution lipase is surveyed live body system: get olive oil emulsion 25ml, 8% bovine bile solution 2ml and water 10ml, put in 100ml triangular flask, add water 2ml, with 0.1mol/L sodium hydroxide solution, regulate pH value to 9.0;
2) .75% ethanol is that sample blank solution lipase is surveyed live body system: get olive oil emulsion 25ml, 8% bovine bile solution 2ml and water 10ml, put in 100ml triangular flask, add 75% alcoholic solution 2ml, with 0.1mol/L sodium hydroxide solution, regulate pH value to 9.0;
3). sample solution lipase is surveyed live body system: get olive oil emulsion 25ml, 8% bovine bile solution 2ml and water 10ml, put in 100ml triangular flask, add respectively sample solution 2ml, with 0.1mol/L sodium hydroxide solution, regulate pH value to 9.0; Sample solution can be a kind of of the water extract of 63 kinds of Chinese herbal medicine or alcohol extract.
C. draw lipase suppression ratio " Y% " and be related to standard curve with pH amplitude of variation " Δ pH ":
During drawing standard curve, first the mensuration enzyme liquid of different gradients is converted into corresponding suppression ratio, suppression ratio reduction formula:
Y(％)＝(X
0-Xn)/X
0
Y (%)-expression lipase suppression ratio
X
0-expression enzyme original liquid concentration (u/ml)
Xn-represents different dilution enzyme liquid concentration (u/ml);
When setting enzyme concentration is zero, suppression ratio is 100%; The suppression ratio of setting enzyme original liquid concentration is 0, proenzyme liquid is become to the mensuration enzyme liquid of 90u/ml, 80u/ml, 70u/ml, 60u/ml, 50u/ml, 40u/ml, 30u/ml, 20u/ml, 10u/ml by certain gradient dilution, corresponding suppression ratio Y% is respectively 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%; According to Specification Curve of Increasing method drawing standard curve, Specification Curve of Increasing method of the present invention is as follows:
Above-mentioned water or 75% ethanol blank solution lipase are surveyed to live body and tie up in 37 ℃ of water-baths and be incubated 10 minutes, with 0.1mol/L sodium hydroxide solution, regulate pH value to 9.0; Precision measures mensuration enzyme liquid 1 parts by volume of above-mentioned different gradient concentrations, adds to respectively blank solution and surveys in live body system, and in 37 ℃ of water-baths, accurate response is 5 minutes, records reaction front and back pH value simultaneously, and calculates reaction front and back pH difference; PH difference while being 100u/ml by enzyme concentration before and after reaction, pH difference when deducting respectively enzyme concentration and being 90u/ml, 80u/ml, 70u/ml, 60u/ml, 50u/ml, 40u/ml, 30u/ml, 20u/ml, 10u/ml, 0u/ml before and after reaction, obtains pH amplitude of variation " Δ pH " value of corresponding different suppression ratio; Take suppression ratio as vertical coordinate, and " Δ pH " value for abscissa, is drawn lipase suppression ratio " Y% " and is related to standard curve with pH amplitude of variation " Δ pH ", and to obtain water or 75% be the lipase suppression ratio computing formula of blank sample solution;
D. the mensuration of assay method and sample solution lipase suppression ratio
Blank solution is measured: above-mentioned water or 75% ethanol blank solution lipase are surveyed to live body and tie up in 37 ℃ of water-baths and be incubated 10 minutes, with 0.1mol/L sodium hydroxide solution, regulate pH value to 9.0; Precision measures proenzyme liquid 1ml and adds to blank solution and survey in live body system, and in 37 ℃ of water-baths, accurate response is 5 minutes, pH value before and after record reaction simultaneously, and calculate the pH difference before and after blank solution reaction, be designated as A1; Separately be taken in water-bath and boil the above-mentioned proenzyme liquid 1ml of 15-30 minute, according to said method, measure, as enzyme blank, pH difference is designated as A0; The pH changing value of blank solution enzyme reaction is Δ A=A1-A0;
Sample solution is measured: sample solution lipase survey live body is tied up in 37 ℃ of water-baths and is incubated 10 minutes, with 0.1mol/L sodium hydroxide solution, regulate pH value to 9.0; Precision measures proenzyme liquid 1ml and adds in sample solution survey live body system, and in 37 ℃ of water-baths, accurate response is 5 minutes, and pH value before and after record reaction simultaneously, and the pH difference of calculation sample solution reaction front and back, be designated as B1; Separately be taken in water-bath and boil the above-mentioned proenzyme liquid 1ml of 20 minutes, according to said method, measure, as enzyme blank, pH difference is designated as B0; The pH changing value of sample solution enzyme reaction is Δ B=B1-B0;
The lipase suppression ratio of sample solution: the pH changing value (Δ B) that deducts sample solution enzyme reaction with the pH changing value (Δ A) of blank solution enzyme reaction, obtain " Δ pH " value of blank solution and sample solution enzyme reaction, be Δ pH=Δ A-Δ B, by the lipase suppression ratio computing formula of the above-mentioned corresponding blank solution of this " Δ pH " value substitution, try to achieve the lipase suppression ratio of working sample, the lipase suppression ratio of sample solution the results are shown in Table 1:
Table 1 Chinese herbal medicine extract is to lipase activity inhibition rate
The power how many representatives of "+" suppress lipase, "+" is more, represents lipase to suppress stronger.
Following embodiment all can realize the effect of above-mentioned experimental example.
The specific embodiment
Embodiment 1: the preparation of Radix Scutellariae water extract
Get baikal skullcap root decoction pieces, prefabricated, pulverize; Add 5 volumes deionized water doubly, boil 30 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 2: the preparation of balcalin extract
Get baikal skullcap root decoction pieces, prefabricated, pulverize; Add 5 volumes, 75% ethanol doubly, supersound extraction 60 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 3: the preparation of Folium camelliae assamicae water extract
Get Folium camelliae assamicae decoction pieces, prefabricated, pulverize; Add 10 volumes deionized water doubly, boil 60 minutes, centrifugal 20 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 4: the preparation of Folium camelliae assamicae alcohol extract
Get Folium camelliae assamicae decoction pieces, prefabricated, pulverize; Add 10 volumes, 95% ethanol doubly, supersound extraction 90 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 5: the preparation of oolong tea water extract
Get oolong tea decoction pieces, prefabricated, pulverize; Add 6 volumes deionized water doubly, boil 40 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 6: the preparation of oolong tea alcohol extract
Get oolong tea decoction pieces, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 30 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 7: the preparation of TIEGUANYIN tea water extract
Get TIEGUANYIN tea decoction pieces, prefabricated, pulverize; Add 6 volumes deionized water doubly, boil 40 minutes, centrifugal 10 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 8: the preparation of TIEGUANYIN tea alcohol extract
Get TIEGUANYIN tea decoction pieces, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 50 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 9: the preparation of Folium Ilicis water extract
Get Folium Ilicis decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boil 50 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 10: the preparation of Folium Ilicis alcohol extract
Get Folium Ilicis decoction pieces, prefabricated, pulverize; Add 7 volumes, 50% ethanol doubly, supersound extraction 80 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 11: the preparation of black tea water extract
Get black tea decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boil 50 minutes, centrifugal 20 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 12: the preparation of black tea alcohol extract
Get black tea decoction pieces, prefabricated, pulverize; Add 7 volumes, 70% ethanol doubly, supersound extraction 70 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 13: the preparation of green tea water extract
Get green tea decoction pieces, prefabricated, pulverize; Add 8 volumes deionized water doubly, boil 50 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 14: the preparation of green tea alcohol extract
Get green tea decoction pieces, prefabricated, pulverize; Add 8 volumes, 95% ethanol doubly, supersound extraction 50 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 15: the preparation of CitrusaurantiumL.Var.amara Engl. water extract
Replace seville orange flower decoction pieces, prefabricated, pulverize; Add 8 volumes deionized water doubly, boil 50 minutes, centrifugal 20 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 16: the preparation of CitrusaurantiumL.Var.amara Engl. alcohol extract
Replace seville orange flower decoction pieces, prefabricated, pulverize; Add 8 volumes, 95% ethanol doubly, supersound extraction 40 minutes, centrifugal 20 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 17: the preparation of crude extract from carthamus tinctorius L. with water
Get Flos Carthami decoction pieces, prefabricated, pulverize; Add 9 volumes deionized water doubly, boil 60 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 18: the preparation of Safflower extract
Get Flos Carthami decoction pieces, prefabricated, pulverize; Add 9 volumes, 95% ethanol doubly, supersound extraction 30 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 19: the preparation of pepper water extract
Get Pericarpium Zanthoxyli decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boil 45 minutes, centrifugal 20 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 20: the preparation of Pericarpium Zanthoxyli alcohol extract
Get Pericarpium Zanthoxyli decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes, centrifugal 20 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 21: the preparation of aloe water extract
Get Aloe decoction pieces, prefabricated, pulverize; Add 5 volumes deionized water doubly, boil 30 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 22: the preparation of Aloe alcohol extract
Get Aloe decoction pieces, prefabricated, pulverize; Add 5 volumes, 75% ethanol doubly, supersound extraction 85 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 23: the preparation of Rhizoma Polygoni Cuspidati water extract
Get Polygonum Cuspidatum, prefabricated, pulverize; Add 10 volumes deionized water doubly, boil 60 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 24: the preparation of Rhizoma Polygoni Cuspidati alcohol extract
Get Polygonum Cuspidatum, prefabricated, pulverize; Add 10 volumes, 95% ethanol doubly, supersound extraction 75 minutes, centrifugal 10 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 25: the preparation of Folium Sennae water extract
Get Folium Sennae decoction pieces, prefabricated, pulverize; Add 6 volumes deionized water doubly, boil 40 minutes, filter, get supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 26: the preparation of Folium Sennae alcohol extract
Get Folium Sennae decoction pieces, prefabricated, pulverize; Add 6 volumes, 60% ethanol doubly, supersound extraction 60 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 27: the preparation of Radix Puerariae water extract
Get Radix Puerariae decoction pieces, prefabricated, pulverize; Add 6 volumes deionized water doubly, boil 40 minutes, 4000rpm/ minute centrifugal, gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 28: the preparation of Radix Puerariae alcohol extract
Get Radix Puerariae decoction pieces, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 80 minutes, centrifugal 20 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 29: the preparation of Danshen
Get salvia piece, prefabricated, pulverize; Add 7 volumes deionized water doubly, boil 50 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 30: the preparation of tanshinol extract
Get salvia piece, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 31: the preparation of Rhizoma Atractylodis Macrocephalae water extract
Get Rhizoma Atractylodis Macrocephalae decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boil 50 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 32: the preparation of Rhizoma Atractylodis Macrocephalae alcohol extract
Get Rhizoma Atractylodis Macrocephalae decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 70 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 33: the preparation of Flos persicae water extract
Get Flos persicae decoction pieces, prefabricated, pulverize; Add 8 volumes deionized water doubly, boil 50 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 34: the preparation of Flos persicae alcohol extract
Get Flos persicae decoction pieces, prefabricated, pulverize; Add 8 volumes, 95% ethanol doubly, supersound extraction 50 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 35: the preparation of Herba Apocyni veneti water extract
Get Herba Apocyni veneti decoction pieces, prefabricated, pulverize; Add 8 volumes deionized water doubly, boil 50 minutes, centrifugal 20 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 36: the preparation of Herba Apocyni veneti alcohol extract
Get Herba Apocyni veneti decoction pieces, prefabricated, pulverize; Add 8 volumes, 95% ethanol doubly, supersound extraction 60 minutes, centrifugal 20 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 37: the preparation of chrysanthemum water extract
Get Flos Chrysanthemi decoction pieces, prefabricated, pulverize; Add 9 volumes deionized water doubly, boil 60 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 38: the preparation of Flos Chrysanthemi alcohol extract
Get Flos Chrysanthemi decoction pieces, prefabricated, pulverize; Add 9 volumes, 95% ethanol doubly, supersound extraction 65 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 39: the preparation of Flos Campsis water extract
Get Flos Campsis decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boil 45 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 40: the preparation of Flos Campsis alcohol extract
Get Flos Campsis decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 41: the preparation of Flos Jasmini Sambac water extract
Get Flos Jasmini Sambac decoction pieces, prefabricated, pulverize; Add 5 volumes deionized water doubly, boil 30 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 42: the preparation of Flos Jasmini Sambac alcohol extract
Get Flos Jasmini Sambac decoction pieces, prefabricated, pulverize; Add 5 volumes, 75% ethanol doubly, supersound extraction 45 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 43: the preparation of Semen Oroxyli water extract
Get Semen Oroxyli decoction pieces, prefabricated, pulverize; Add 10 volumes deionized water doubly, boil 60 minutes, centrifugal 10 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 44: the preparation of Semen Oroxyli alcohol extract
Get Semen Oroxyli decoction pieces, prefabricated, pulverize; Add 10 volumes, 95% ethanol doubly, supersound extraction 60 minutes, centrifugal 10 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 45: the preparation of Semen Vitis viniferae water extract
Get Semen Vitis viniferae decoction pieces, prefabricated, pulverize; Add 6 volumes deionized water doubly, boil 40 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 46: the preparation of Semen Vitis viniferae alcohol extract
Get Semen Vitis viniferae decoction pieces, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 90 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 47: the preparation of Flos Trollii water extract
Depletion Flos Nelumbinis decoction pieces, prefabricated, pulverize; Add 6 volumes deionized water doubly, boil 40 minutes, centrifugal 20 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 48: the preparation of Flos Trollii alcohol extract
Depletion Flos Nelumbinis decoction pieces, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 60 minutes, centrifugal 20 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 49: the preparation of lilac water extract
Get Flos Caryophylli decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boil 50 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 50: the preparation of syringyl alcohol extract
Get Flos Caryophylli decoction pieces, prefabricated, pulverize; Add 7 volumes, 75% ethanol doubly, supersound extraction 75 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 51: the preparation of Flos Lonicerae water extract
Extracting honeysuckle decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boil 50 minutes, centrifugal 10 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 52: the preparation of Flos Lonicerae alcohol extract
Extracting honeysuckle decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes, centrifugal 10 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 53: the preparation of Radix Cynanchi Atrati water extract
Get Radix Cynanchi Atrati decoction pieces, prefabricated, pulverize; Add 8 volumes deionized water doubly, boil 50 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 54: the preparation of Radix Cynanchi Atrati alcohol extract
Get Radix Cynanchi Atrati decoction pieces, prefabricated, pulverize; Add 8 volumes, 95% ethanol doubly, supersound extraction 70 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 55: the preparation of Radix Sophorae Flavescentis water extract
Get Radix Sophorae Flavescentis decoction pieces, prefabricated, pulverize; Add 8 volumes deionized water doubly, boil 50 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 56: the preparation of Radix Sophorae Flavescentis alcohol extract
Get Radix Sophorae Flavescentis decoction pieces, prefabricated, pulverize; Add 8 volumes, 95% ethanol doubly, supersound extraction 80 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 57: the preparation of Herba Cassiae Nomamis water extract
Water intaking Fructus Gleditsia decoction pieces, prefabricated, pulverize; Add 9 volumes deionized water doubly, boil 60 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 58: the preparation of Herba Cassiae Nomamis alcohol extract
Water intaking Fructus Gleditsia decoction pieces, prefabricated, pulverize; Add 9 volumes, 95% ethanol doubly, supersound extraction 60 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 59: the preparation of Folium Artemisiae Argyi water extract
Get Folium Artemisiae Argyi decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boil 45 minutes, centrifugal 10 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 60: the preparation of Folium Artemisiae Argyi alcohol extract
Get Folium Artemisiae Argyi decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes, centrifugal 10 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 61: the preparation of Radix Ginseng water extract
Get Radix Ginseng decoction pieces, prefabricated, pulverize; Add 5 volumes deionized water doubly, boil 30 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 62: the preparation of panaxynol extract
Get Radix Ginseng decoction pieces, prefabricated, pulverize; Add 5 volumes, 75% ethanol doubly, supersound extraction 60 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 63: the preparation of Flos Sophorae Immaturus water extract
Get Flos Sophorae Immaturus decoction pieces, prefabricated, pulverize; Add 10 volumes deionized water doubly, boil 60 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 64: the preparation of Flos Sophorae Immaturus alcohol extract
Get Flos Sophorae Immaturus decoction pieces, prefabricated, pulverize; Add 10 volumes, 95% ethanol doubly, supersound extraction 70 minutes, centrifugal 20 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 65: the preparation of Rhizoma Fagopyri Dibotryis water extract
Depletion Semen Fagopyri Esculenti decoction pieces, prefabricated, pulverize; Add 6 volumes deionized water doubly, boil 40 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 66: the preparation of Rhizoma Fagopyri Dibotryis alcohol extract
Depletion Semen Fagopyri Esculenti decoction pieces, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 60 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 67: the preparation of Rhizoma Alismatis water extract
Get Rhizoma Alismatis decoction pieces, prefabricated, pulverize; Add 6 volumes deionized water doubly, boil 40 minutes, centrifugal 20 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 68: the preparation of alisol extract
Get Rhizoma Alismatis decoction pieces, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 60 minutes, centrifugal 20 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 69: the preparation of Fructus Aurantii water extract
Get Fructus Aurantii Praeparata, prefabricated, pulverize; Add 7 volumes deionized water doubly, boil 50 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 70: the preparation of Fructus Aurantii alcohol extract
Get Fructus Aurantii Praeparata, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 71: the preparation of Radix Achyranthis Bidentatae water extract
Get Radix Achyranthis Bidentatae decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boil 50 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 72: the preparation of Radix Achyranthis Bidentatae alcohol extract
Get Radix Achyranthis Bidentatae decoction pieces, prefabricated, pulverize; Add 7 volumes, 65% ethanol doubly, supersound extraction 75 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 73: the preparation of Fructus Aurantii Immaturus water extract
Get Fructus Aurantii Immaturus decoction pieces, prefabricated, pulverize; Add 8 volumes deionized water doubly, boil 50 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 74: the preparation of Fructus Aurantii Immaturus alcohol extract
Get Fructus Aurantii Immaturus decoction pieces, prefabricated, pulverize; Add 8 volumes, 95% ethanol doubly, supersound extraction 60 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 75: the preparation of Fructus Chebulae's water extract
Get Fructus Chebulae's decoction pieces, prefabricated, pulverize; Add 8 volumes deionized water doubly, boil 50 minutes, centrifugal 10 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 76: the preparation of Fructus Chebulae's alcohol extract
Get Fructus Chebulae's decoction pieces, prefabricated, pulverize; Add 8 volumes, 90% ethanol doubly, supersound extraction 60 minutes, centrifugal 10 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 77: the preparation of Caulis Polygoni Multiflori water extract
Get Caulis Polygoni Multiflori decoction pieces, prefabricated, pulverize; Add 9 volumes deionized water doubly, boil 60 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 78: the preparation of Caulis Polygoni Multiflori alcohol extract
Get Caulis Polygoni Multiflori decoction pieces, prefabricated, pulverize; Add 9 volumes, 95% ethanol doubly, supersound extraction 60 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 79: the preparation of Radix Aucklandiae water extract
Get Radix Aucklandiae decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boil 45 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 80: the preparation of Aucklandiae alcohol extractive
Get Radix Aucklandiae decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 50 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 81: the preparation of Folium Ginkgo water extract
Get Folium Ginkgo decoction pieces, prefabricated, pulverize; Add 5 volumes deionized water doubly, boil 30 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 82: the preparation of ethanol extracts from ginkgo biloba leaves
Get Folium Ginkgo decoction pieces, prefabricated, pulverize; Add 5 volumes, 75% ethanol doubly, supersound extraction 45 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 83: the preparation of Rhizoma Osmundae water extract
Get Rhizoma Osmundae decoction pieces, prefabricated, pulverize; Add 10 volumes deionized water doubly, boil 60 minutes, centrifugal 20 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 84: the preparation of Rhizoma Osmundae alcohol extract
Get Rhizoma Osmundae decoction pieces, prefabricated, pulverize; Add 10 volumes, 50% ethanol doubly, supersound extraction 60 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 85: the preparation of Cortex Eucommiae water extract
Get Cortex Eucommiae decoction pieces, prefabricated, pulverize; Add 6 volumes deionized water doubly, boil 40 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 86: the preparation of eucommoil extract
Get Cortex Eucommiae decoction pieces, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 60 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 87: the preparation of Pollen Tyjphae water extract
Get Pollen Tyjphae decoction pieces, prefabricated, pulverize; Add 6 volumes deionized water doubly, boil 40 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 88: the preparation of Pollen Tyjphae alcohol extract
Get Pollen Tyjphae decoction pieces, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 50 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 89: the preparation of Fructus Trichosanthis water extract
Get Fructus Trichosanthis decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boil 50 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 90: the preparation of Fructus Trichosanthis alcohol extract
Get Fructus Trichosanthis decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 91: the preparation of Folium Mori water extract
Get Folium Mori decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boil 50 minutes, centrifugal 20 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 92: the preparation of Folium Mori alcohol extract
Get Folium Mori decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes, centrifugal 10 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 93: the preparation of Cortex Mori water extract
Get Cortex Mori decoction pieces, prefabricated, pulverize; Add 8 volumes deionized water doubly, boil 50 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 94: the preparation of Cortex Mori alcohol extract
Get Cortex Mori decoction pieces, prefabricated, pulverize; Add 8 volumes, 90% ethanol doubly, supersound extraction 80 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 95: the preparation of Fructus Lycii water extract
Get Fructus Lycii decoction pieces, prefabricated, pulverize; Add 8 volumes deionized water doubly, boil 50 minutes, centrifugal 20 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 96: the preparation of Folium Nelumbinis water extract
Get Folium Nelumbinis decoction pieces, prefabricated, pulverize; Add 9 volumes deionized water doubly, boil 60 minutes, filter, get filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 97: the preparation of Radix Platycodonis water extract
Get Radix Platycodonis decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boil 45 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 98: the preparation of rhababerone extract
Get Radix Et Rhizoma Rhei decoction piece, prefabricated, pulverize; Add 8 volumes, 95% ethanol doubly, supersound extraction 75 minutes, centrifugal 10 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 99: the preparation of glycyrol extract
Extracting liquorice decoction pieces, prefabricated, pulverize; Add 9 volumes, 95% ethanol doubly, supersound extraction 60 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 100: the preparation of Rhizoma Chuanxiong alcohol extract
Get Rhizoma Chuanxiong decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 70 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 101: the preparation of Fructus Chaenomelis alcohol extract
Get pawpaw decoction pieces, prefabricated, pulverize; Add 5 volumes, 75% ethanol doubly, supersound extraction 60 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 102: the preparation of milletol extract
Get Caulis Spatholobi decoction pieces, prefabricated, pulverize; Add 10 volumes, 95% ethanol doubly, supersound extraction 60 minutes, centrifugal 10 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 103: the preparation of Cortex Magnoliae Officinalis alcohol extract
Get Medicinal Magnolia Bark, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 60 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 104: the preparation of Cortex Moutan alcohol extract
Get paeonol piece, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 60 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 105: the preparation of Herb Gynostemmae Pentaphylli alcohol extract
Get Herb Gynostemmae Pentaphylli decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 106: the preparation of curcumol extract
Get Rhizoma Curcumae Longae decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 90 minutes, centrifugal 10 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 107: the preparation of Notopterol extract
Get Rhizoma Et Radix Notopterygii decoction pieces, prefabricated, pulverize; Add 8 volumes, 95% ethanol doubly, supersound extraction 60 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 108: the preparation of Radix Polygoni Multiflori alcohol extract
Get Radix Polygoni Multiflori decoction pieces, prefabricated, pulverize; Add 8 volumes, 95% ethanol doubly, supersound extraction 90 minutes, centrifugal 10 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 109: the preparation of Semen Arecae alcohol extract
Get Semen Arecae decoction pieces, prefabricated, pulverize; Add 9 volumes, 95% ethanol doubly, supersound extraction 60 minutes, filters, and gets filtrate; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
Embodiment 110: the preparation of Ramulus Cinnamomi alcohol extract
Get Ramulus Cinnamomi decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes, centrifugal 15 minutes of 4000r/min, gets supernatant; By conventional method, make regular dosage form, for the preparation of lipase inhibitor.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (4)
1. the water extract of Chinese herbal medicine CitrusaurantiumL.Var.amara Engl. has the application in the medicine of lipase activity inhibition as unique active ingredient in preparation, and described lipase is pancreatic lipase.
2. application according to claim 1, is characterized in that, the water extract of described Chinese herbal medicine CitrusaurantiumL.Var.amara Engl. is prepared by the following method: replace seville orange flower herbal decoction piece, and prefabricated, pulverize; Add 5-10 volume deionized water doubly, boil 30-60 minute, filter, or the centrifugal 10-20 minute of 4000r/min, get filtrate or centrifuged supernatant and obtain described water extract.
3. application according to claim 2, is characterized in that, the water extract of described Chinese herbal medicine CitrusaurantiumL.Var.amara Engl. is prepared by the following method: get described CitrusaurantiumL.Var.amara Engl. herbal decoction piece, and prefabricated, pulverize; Add 7 volumes deionized water doubly, boil 45 minutes, filter, or centrifugal 15 minutes of 4000r/min, get filtrate or centrifuged supernatant and obtain described water extract.
4. application according to claim 2, is characterized in that, the water extract of described Chinese herbal medicine CitrusaurantiumL.Var.amara Engl. is prepared by the following method: get described CitrusaurantiumL.Var.amara Engl. herbal decoction piece, and prefabricated, pulverize; Add 8 volumes deionized water doubly, boil 50 minutes, centrifugal 20 minutes of 4000r/min, gets supernatant and obtains described water extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110317409.2A CN102357142B (en) | 2007-08-07 | 2007-08-07 | Use of extract of Chinese herbal medicine seville orange flower in preparation of drug for losing weight and reducing blood fat or drug for inhibiting lipase activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110317409.2A CN102357142B (en) | 2007-08-07 | 2007-08-07 | Use of extract of Chinese herbal medicine seville orange flower in preparation of drug for losing weight and reducing blood fat or drug for inhibiting lipase activity |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007101200147A Division CN101361886B (en) | 2007-08-07 | 2007-08-07 | Chinese herbal medicine extract with lipase activity inhibition and new use thereof |
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| CN102357142A CN102357142A (en) | 2012-02-22 |
| CN102357142B true CN102357142B (en) | 2014-03-12 |
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| CN201110317409.2A Expired - Fee Related CN102357142B (en) | 2007-08-07 | 2007-08-07 | Use of extract of Chinese herbal medicine seville orange flower in preparation of drug for losing weight and reducing blood fat or drug for inhibiting lipase activity |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106562988B (en) * | 2016-11-11 | 2020-02-18 | 华南理工大学 | Application of substituted calyx polysaccharide in preparation of weight-reducing medicine |
| CN106539944A (en) * | 2016-11-11 | 2017-03-29 | 华南理工大学 | A kind of effective ingredient of calyx from generation to generation and preparation method and the application in slimming medicine is prepared |
| CN106728187A (en) * | 2016-11-11 | 2017-05-31 | 华南理工大学 | A kind of extract of calyx from generation to generation and preparation method and the application in slimming medicine is prepared |
| TWI746917B (en) * | 2018-12-22 | 2021-11-21 | 大江生醫股份有限公司 | Use of citrus aurantium flower extract for reducing skin aging and fat content |
| TWI794575B (en) * | 2019-09-10 | 2023-03-01 | 大江生醫股份有限公司 | Use of compound for preventing and/or treating of hyperlipidemia |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1191680A (en) * | 1998-03-30 | 1998-09-02 | 项玉昌 | Three-flower slimming tea |
| CN1363367A (en) * | 2002-01-18 | 2002-08-14 | 北京大学 | Active components of Chinese-medicinal medicine with weight-losing and hypolipemic actions and its relative medicine |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3953847B2 (en) * | 2002-03-11 | 2007-08-08 | 花王株式会社 | Lipolysis accelerator |
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2007
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1191680A (en) * | 1998-03-30 | 1998-09-02 | 项玉昌 | Three-flower slimming tea |
| CN1363367A (en) * | 2002-01-18 | 2002-08-14 | 北京大学 | Active components of Chinese-medicinal medicine with weight-losing and hypolipemic actions and its relative medicine |
Non-Patent Citations (3)
| Title |
|---|
| JP特开2002-326947A 2002.11.15 |
| Niti Sharma et al..screening of some medicinal plants for anti-lipase acitivity.《Journal of Ethnopharmacology》.2005,第97卷(第3期),第453-456页. |
| screening of some medicinal plants for anti-lipase acitivity;Niti Sharma et al.;《Journal of Ethnopharmacology》;20051231;第97卷(第3期);第453-456页，尤其是第454页Table 1,第455页第2.2节，第2.3节 * |
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