CN102335252A - Oral targeting preparation of compound three-root extract for resisting colorectal cancer and preparation method thereof - Google Patents
Oral targeting preparation of compound three-root extract for resisting colorectal cancer and preparation method thereof Download PDFInfo
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Abstract
The invention discloses an oral targeting preparation of a compound three-root extract for resisting a colorectal cancer and a preparation method thereof. The preparation method comprises the following steps of: at first, compounding medicaments with phospholipid to form liposome; and then coating the liposome containing the medicaments by using a coliform bacteria biodegradable material, a retarded coating material, or a pH-sensitive coating material to obtain the targeting preparation. With the adoption of the preparation, the liposome is released at a colon position and absorbed by colorectal cancer cells so that a tumor is directly killed. According to the preparation provide by the invention, the target concentration and the action effects of Chinese medicinal herb ingredients are improved by using special tissues and cells to dually locate a target; in addition, the preparation provided by the invention is applied to the Chinese medicinal herb ingredients which are easy to damage, and therefore the stability of the Chinese medicinal herb ingredients is improved, and defects that the Chinese medicinal herb ingredients have short and weak effects are overcome.
Description
Technical field
The present invention relates to medical manufacturing field, be specifically related to oral targeting preparation of a kind of herbal mixture extract Chinese People's Anti-Japanese Military and Political College's intestinal cancer and preparation method thereof.
Background technology
Colorectal cancer is the malignant tumor of digestive tract of high harm, and sickness rate has accounted for the 4th of kinds of tumor.Met or exceeded the average level of western developed country at metropolitan colorectal cancer annual morbidities such as Beijing, Shanghai, colorectal cancer becomes second malignant tumor occurred frequently that is only second to pulmonary carcinoma.In recent years, the incidence of colorectal ascendant trend is fairly obvious, according to statistics; The international standard of the rate of climb of China morbidity considerably beyond 2%, a year morbidity rate of increase reaches 4.2%, presses on towards 5%; Annual new cases are up to 400,000, and this wherein much all is 30～40 years old a middle age.Operative treatment is classical efficacious therapy method, but operation causes the serious reduction of patients ' life quality.Toxic and side is very remarkable, and the treatment by Chinese herbs colorectal cancer has special advantages.
The traditional Chinese medical science thinks that colorectal cancer belongs to categories such as " mass in the abdomen ", " spouting bleeding from anus " more, and mostly its cause of disease is eating and drinking without temperance, and addiction to greasy and sweet foods, scorching or unclean thing cause the dysfunction of the spleen, damp and hotly accumulates malicious tenesmus large intestine, thermal burn intestinal network, and poison is evil to be become carbuncle and carcinoma takes place gradually.Etiology and pathogenesis and " insufficiency of the spleen ", " noxious dampness internal resistance " relation maximum so catch disease essence, adopt clearing away heat-damp and promoting diuresis, eliminating toxic substances to remove stasis, tonifying speen and tonifying kidney Therapeutic Method, have obvious therapeutic effect.The prescription that three prescriptions of compound recipe are made up of polygoni cuspidati,radix, Radix Actinidiae Chinensis, Radix Gei japonici cures mainly digestive tract tumor such as colorectal cancer, gastric cancer.The CN1679795A patent report preparation technology of three active components of compound recipe.
Mostly the Chinese medicine active component is Polyphenols, glucosides class, is prone to decompose in oral back and destroys.The active component that compound recipe is three is emodin, Polyphenols (Ye Yong etc., the anti tumor activity in vitro of Radix Gei japonici extract, Zhejiang University of Traditional Chinese Medicine's journal, 2007,31 (3): 372-373), be prone to by enzymolysis, or oxidation under alkali condition.Short at the oral administration after effect time, do not reach target position and promptly lost efficacy.
The targeting preparation of Chinese medicine extract has only less report; Some patents such as publication number CN1480193B; CN1634299B; CN101991720A just adopts targeting material coating to form tablet, pill or capsule, does not still have the report that liposome carries out targeting material coating outward again, does not also have herbal mixture extract targeting preparation report.
Summary of the invention
The shortcoming that the objective of the invention is to overcome prior art provides oral colorectal cancer targeting preparation of a kind of herbal mixture extract and preparation method thereof with not enough.This method is to herbal mixture three root extracts, proposes to prepare liposome in advance, carries out multiple targeting material coating again, can realize that the intestinal tissue position discharges, and directly absorbed by colorectal cancer cells, brings into play better action effect.
The object of the invention is realized through following technical proposals:
The method for preparing of the oral targeting preparation of compound recipe three root extracts of a kind of Chinese People's Anti-Japanese Military and Political College intestinal cancer comprises the steps:
(1) compound recipe three root extracts and phospholipid are mixed by weight 1: 1～3, join in ethyl acetate or chloroform or n-butyl alcohol or the ethanol, 60～80 ℃ of reflux 1～2 hour are clarified to solution; With 60～80 ℃ of vacuum concentration of solution and dry 1～5 hour, promptly get compound recipe three root extract phosphatide complexes again;
(2) the compound recipe three root extract phosphatide complexes that step (1) made, with phospholipid, cholesterol respectively by weight 10～35%, 30～65%; After 15～40% mixing; Join in ethyl acetate or chloroform or n-butyl alcohol or the ethanol, 30～60 ℃ of heated and stirred are to the solution clear; Again with 30～60 ℃ of vacuum concentration of solution to jelly, add the phosphate buffer of pH4～10, fully after the aquation preliminary liposome;
(3) in the preliminary liposome that step (2) makes by weight the mannitol or alginic acid or the glucose that add 5～20%, fully after the dissolving, lyophilization promptly gets compound recipe three root extract liposomees;
(4) with coating material, plasticizer, solvent by weight percentage 1～10%, 0.5～5%, 85～98.5% mixed dissolutions process the coating solution of good fluidity; Adopt above-mentioned coating solution that compound recipe three root extract liposomees are carried out coating, coating weightening finish 10～20% promptly makes targeting preparation.The double-decker that this targeting preparation is made up of internal layer liposome structure and outer targeting coating material.
The said coating material of step (4) is one or more in Eudragit S100, acrylic resin III, hydroxypropyl methylcellulose, ethyl cellulose, pectin, the guar gum.
The said plasticizer of step (4) is a Polyethylene Glycol, one or more in diethyl phthalate, the triethyl citrate.
The said solvent of step (4) is one or more in acetone, ethanol, the isopropyl alcohol.
The effective ingredient of said compound recipe three root extracts of step (1) comprises the emodin and the polyphenolic substance of polygoni cuspidati,radix, Radix Actinidiae Chinensis and Radix Gei japonici mixed extraction.
The method for preparing of said compound recipe three root extracts: get polygoni cuspidati,radix, Radix Actinidiae Chinensis, Radix Gei japonici medical material; Mix by 1: 1: 1 quality, added 80 ℃ of heating and refluxing extraction of mixed medicinal materials 10 times of amounts, 80% ethanol 2 hours, filter; Filtering residue added 80 ℃ of heating and refluxing extraction of mixed medicinal materials 5 times of amounts, 80% ethanol 1 hour; Filter, 1/2 of 60 ℃ of vacuum concentration to original volumes of merging filtrate add macroporous resin D101 absorption 12 hours; Take out macroporous resin and use 50% ethanol elution, 60 ℃ of vacuum concentration of eluent and drying 3 hours get compound recipe three root extracts.
The present invention has following advantage and effect with respect to prior art:
(1) the present invention is a medicine material with herbal mixture active component or composition, processes targeting preparation, can realize multipath, multimachine reason therapeutical effect to the target position tumor.
(2) targeting preparation of the present invention is encapsulated by internal layer liposome and outer biodegradation material or time lag type coating material or pH sensitive material and to form, and can discharge at the large intestine position, and absorbed by colorectal cancer cells.Particular organization and the effect of specific cells dual-target have been realized.
(3) targeting preparation of the present invention overcomes the shortcoming of Chinese medicine ingredients instability, fugitive, weak effect, has increased the stability and the target position concentration that are subject to destructive Chinese medicine ingredients, directly brings into play the effect of Chinese People's Anti-Japanese Military and Political College's intestinal cancer, has improved the Chinese medicine action effect.
The specific embodiment
Below in conjunction with embodiment the present invention is described in further detail, but embodiment of the present invention is not limited thereto.
The preparation of compound recipe three root extracts: get polygoni cuspidati,radix, Radix Actinidiae Chinensis, Radix Gei japonici medical material; By 1: 1: 1 weighing 900g, added 80 ℃ of heating and refluxing extraction of 9kg 80% ethanol 2 hours, filter; Filtering residue added 80 ℃ of heating and refluxing extraction of 4.5kg 80% ethanol 1 hour; Filter, 1/2 of 60 ℃ of vacuum concentration to original volumes of merging filtrate add 1kg macroporous resin D101 absorption 12 hours.Take out macroporous resin and use 50% ethanol elution, 60 ℃ of vacuum concentration of eluent and drying 3 hours get compound recipe three root extract 130g.
Embodiment 1
(1) compound recipe three root extract 1g mix with phosphatidase 13 g, add ethyl acetate 50ml, and 60 ℃ of reflux 1 hour are clarified to solution.With 60 ℃ of vacuum concentration of solution and dry 1 hour, promptly get compound recipe three root extract phosphatide complexes powder 3.8g again.
(2) with compound recipe three root extract phosphatide complexes 1g, mix with phosphatidase 12 .5g, cholesterol 1.5g, add ethyl acetate 50ml, 40 ℃ of heated and stirred are to the solution clear.Again with 60 ℃ of vacuum concentration of solution to jelly, add the phosphate buffer 10ml of pH4, fully after the aquation preliminary liposome.
(3) get preliminary liposome 10g, to wherein adding 1g mannitol, fully after the dissolving, lyophilization gets compound recipe three root extract lipid freeze-dry powder 5.7g.
(4) get 2g Eudragit S100,1g Polyethylene Glycol and add acetone 100g and mix fully to stir and make coating solution, then compound recipe three root extract liposomees are carried out coating, coating weightening finish 20%.
Embodiment 2
(1) compound recipe three root extract 1g mix with phosphatidase 11 g, add chloroform 50ml, and 60 ℃ of reflux 2 hours are clarified to solution.With 60 ℃ of vacuum concentration of solution and dry 3 hours, promptly get compound recipe three root extract phosphatide complexes powder 1.7g again.
(2) with compound recipe three root extract phosphatide complexes 1g, mix with phosphatidase 15 g, cholesterol 2g, add chloroform 50ml, 30 ℃ of stirrings are to the solution clear.Again with 50 ℃ of vacuum concentration of solution to jelly, add the phosphate buffer 50ml of pH10, fully after the aquation preliminary liposome.
(3) in preliminary liposome 50g, add the 2.5g alginic acid, fully after the dissolving, lyophilization gets compound recipe three root extract lipid freeze-dry powder 10g.
(4) get 5g hydroxypropyl methylcellulose, fully stirring of 1g diethyl phthalate adding ethanol 100g mixing.Compound recipe three root extract liposomees are carried out coating, coating weightening finish 15%.
Embodiment 3
(1) compound recipe three root extract 1g mix with phosphatidase 12 g, add n-butyl alcohol 50ml, and 80 ℃ of reflux 2 hours are clarified to solution.With 80 ℃ of vacuum concentration of solution and dry 5 hours, promptly get compound recipe three root extract phosphatide complexes powder 2.5g again.
(2) with compound recipe three root extract phosphatide complexes 1g, mix with phosphatidase 12 g, cholesterol 2g, add n-butyl alcohol 50ml, 60 ℃ of stirrings are to the solution clear.Again with 60 ℃ of vacuum concentration of solution to jelly, add the phosphate buffer 10ml of pH6, fully after the aquation preliminary liposome.
(3) in preliminary liposome 10g, add the 2g glucose, fully after the dissolving, lyophilization gets compound recipe three root extract lipid freeze-dry powder 6.5g.
(4) get 10g pectin, fully stirring of 5g triethyl citrate adding isopropyl alcohol 100g mixing.Compound recipe three root extract liposomees are carried out coating, coating weightening finish 18%.
Embodiment 4
(1) compound recipe three root extract 1g mix with phosphatidase 11 .5g, add ethanol 50ml, and 70 ℃ of reflux 1 hour are clarified to solution.With 70 ℃ of vacuum concentration of solution and dry 2 hours, promptly get compound recipe three root extract phosphatide complexes powder 2.2g again.
(2) with compound recipe three root extract phosphatide complexes 1g, mix with phosphatidase 11 g, cholesterol 1g, add ethanol 50ml, 50 ℃ of heated and stirred are to the solution clear.Again with 60 ℃ of vacuum concentration of solution to jelly, add the phosphate buffer 10ml of pH7, fully after the aquation preliminary liposome.
(3) in preliminary liposome 10g, add 2g mannitol, fully after the dissolving, lyophilization gets compound recipe three root extract lipid freeze-dry powder 4.6g.
(4) get 8g acrylic resin III, fully stirring of 2g diethyl phthalate adding acetone 100g mixing.Compound recipe three root extract liposomees are carried out coating, coating weightening finish 16%.
Embodiment 5
(1) compound recipe three root extract 1g mix with phosphatidase 12 .5g, add chloroform 50ml, and 60 ℃ of reflux 2 hours are clarified to solution.With 60 ℃ of vacuum concentration of solution and dry 3 hours, promptly get compound recipe three root extract phosphatide complexes powder 3.3g again.
(2) with compound recipe three root extract phosphatide complexes 1g, mix with phosphatidase 13 g, cholesterol 1g, add chloroform 50ml, 30 ℃ of stirrings are to the solution clear.Again with 50 ℃ of vacuum concentration of solution to jelly, add the phosphate buffer 10ml of pH8, fully after the aquation preliminary liposome.
(3) in preliminary liposome 10g, add the 1.5g alginic acid, fully after the dissolving, lyophilization gets compound recipe three root extract lipid freeze-dry powder 6.2g.
(4) get 4g ethyl cellulose, fully stirring of 1.5g Polyethylene Glycol adding isopropyl alcohol 100g mixing.Compound recipe three root extract liposomees are carried out coating, coating weightening finish 13%.
Embodiment 6
(1) compound recipe three root extract 1g mix with phosphatidase 11 g, add ethyl acetate 50ml, and 60 ℃ of reflux 1 hour are clarified to solution.With 60 ℃ of vacuum concentration of solution and dry 1 hour, promptly get compound recipe three root extract phosphatide complexes powder 1.5g again.
(2) with compound recipe three root extract phosphatide complexes 1g, mix with phosphatidase 11 .5g, cholesterol 0.5g, add ethyl acetate 50ml, 40 ℃ of heated and stirred are to the solution clear.Again with 60 ℃ of vacuum concentration of solution to jelly, add the phosphate buffer 10ml of pH5, fully after the aquation preliminary liposome.
(3) in preliminary liposome 10g, add the 2g glucose, fully after the dissolving, lyophilization gets compound recipe three root extract lipid freeze-dry powder 4.5g.
(4) get 1g guar gum, fully stirring of 1g triethyl citrate adding ethanol 100g mixing.Compound recipe three root extract liposomees are carried out coating, coating weightening finish 10%.
Embodiment 7
The release degree experiment of the compound recipe three root extract targeting preparations that embodiment 1-6 makes
Method:, investigate the burst size of targeting preparation in simulated gastric fluid and intestinal juice, in solid content amount in the dissolution fluid according to 2010 editions two appendix XD drug release determinations of Chinese Pharmacopoeia method.
The result: targeting preparation did not discharge in simulated gastric fluid in 2 hours, and 2～3 hours begin to discharge in the pH7.0 simulated intestinal fluid, and dissolution can reach 80～87% in 5 hours.The result sees table one.This shows that said preparation has the effect that targeting discharges at the large intestine position.
The dissolution of table one compound recipe three root extract targeting preparations in simulated intestinal fluid
Embodiment 8
The targeting preparation that embodiment 1～6 makes is to the growth inhibited experiment of bringing out property of rat colorectal cancer
Method: 90 rats, male, body weight 60～80g.Be divided into 9 groups at random: normal control group, 1～7 group of modeling group and administration group, 10 every group.Normal control group cervical region subcutaneous injection equivalent normal saline, modeling group and administration group cervical region subcutaneous injection dimethylhydrazine (DMH) 20mg/kg, 1 time weekly, continuous 9 weeks.The administration group gives embodiment 1～6 preparation and compound recipe three root extract 30mg/kg respectively, and administration every day is irritated stomach 1 time.In experiment the 32nd all sacrificed by decapitation rats, open the abdominal cavity rapidly, isolate whole large intestine, vertically cut open, use the normal saline flushing intestinal contents, a situation arises to observe every rat large bowel neoplasm.Except that calculating the tumor number, also use vernier caliper measurement tumor volume, calculate tumour inhibiting rate.
Tumour inhibiting rate=(modeling group tumor volume-administration group tumor volume)/modeling group tumor volume * 100
The result: same dose compound recipe three root extract targeting preparations are significantly higher than compound recipe three root extract groups and modeling group to the colorectal cancer tumour inhibiting rate, point out it to have good colorectal cancer targeting and better antitumous effect.The result sees table two.
Table two compound recipe three root extract targeting preparations are to the inhibition experiment of bringing out property colorectal cancer
Annotate: compare * P＜0.05, * * P＜0.01 with the modeling group
The foregoing description is a preferred implementation of the present invention; But embodiment of the present invention is not restricted to the described embodiments; Other any do not deviate from change, the modification done under spirit of the present invention and the principle, substitutes, combination, simplify; All should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (7)
1. the method for preparing of the oral targeting preparation of compound recipe three root extracts of Chinese People's Anti-Japanese Military and Political College's intestinal cancer is characterized in that, comprises the steps:
(1) compound recipe three root extracts and phospholipid are mixed by weight 1: 1～3, join in ethyl acetate or chloroform or n-butyl alcohol or the ethanol, 60～80 ℃ of reflux 1～2 hour are clarified to solution; With 60～80 ℃ of vacuum concentration of solution and dry 1～5 hour, promptly get compound recipe three root extract phosphatide complexes again;
(2) the compound recipe three root extract phosphatide complexes that step (1) made, with phospholipid, cholesterol respectively by weight 10～35%, 30～65%; After 15～40% mixing; Join in ethyl acetate or chloroform or n-butyl alcohol or the ethanol, 30～60 ℃ of heated and stirred are to the solution clear; Again with 30～60 ℃ of vacuum concentration of solution to jelly, add the phosphate buffer of pH4～10, fully after the aquation preliminary liposome;
(3) in the preliminary liposome that step (2) makes by weight the mannitol or alginic acid or the glucose that add 5～20%, fully after the dissolving, lyophilization promptly gets compound recipe three root extract liposomees;
(4) with coating material, plasticizer, solvent by weight percentage 1～10%, 0.5～5%, 85～98.5% mixed dissolutions process coating solution; Adopt above-mentioned coating solution that compound recipe three root extract liposomees are carried out coating, coating weightening finish 10～20% promptly makes targeting preparation.
2. method for preparing according to claim 1 is characterized in that, the said coating material of step (4) is one or more in Eudragit S100, acrylic resin III, hydroxypropyl methylcellulose, ethyl cellulose, pectin, the guar gum.
3. method for preparing according to claim 1 and 2 is characterized in that, the said plasticizer of step (4) is a Polyethylene Glycol, one or more in diethyl phthalate, the triethyl citrate.
4. method for preparing according to claim 3 is characterized in that, the said solvent of step (4) is one or more in acetone, ethanol, the isopropyl alcohol.
5. method for preparing according to claim 4 is characterized in that, the effective ingredient of said compound recipe three root extracts of step (1) comprises the emodin and the polyphenolic substance of polygoni cuspidati,radix, Radix Actinidiae Chinensis and Radix Gei japonici mixed extraction.
6. method for preparing according to claim 5 is characterized in that, the method for preparing of said compound recipe three root extracts: get polygoni cuspidati,radix, Radix Actinidiae Chinensis, Radix Gei japonici medical material; Mix by 1: 1: 1 quality, added 80 ℃ of heating and refluxing extraction of mixed medicinal materials 10 times of amounts, 80% ethanol 2 hours, filter; Filtering residue added 80 ℃ of heating and refluxing extraction of mixed medicinal materials 5 times of amounts, 80% ethanol 1 hour; Filter, 1/2 of 60 ℃ of vacuum concentration to original volumes of merging filtrate add macroporous resin D101 absorption 12 hours; Take out macroporous resin and use 50% ethanol elution, 60 ℃ of vacuum concentration of eluent and drying 3 hours get compound recipe three root extracts.
7. according to the targeting preparation of any said method preparation of claim 1～6, it is characterized in that the double-decker that it is made up of internal layer liposome structure and outer targeting coating material.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111110863A (en) * | 2020-01-21 | 2020-05-08 | 郑州大学 | Rhein phospholipid complex, preparation method and application thereof, rhein phospholipid complex long-circulating liposome and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1679796A (en) * | 2005-01-04 | 2005-10-12 | 华南理工大学 | Anti-cancer compound preparation of giant knotweed, Tengli root and thinleaf adina root and its making method and use |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1679796A (en) * | 2005-01-04 | 2005-10-12 | 华南理工大学 | Anti-cancer compound preparation of giant knotweed, Tengli root and thinleaf adina root and its making method and use |
Non-Patent Citations (2)
| Title |
|---|
| 刘因华: "抗肿瘤药物的研究现状与发展前景", 《中国医药指南》, 30 September 2007 (2007-09-30) * |
| 廖增华 摘: "中医中药治疗肿瘤的近况", 《新医学》, no. 04, 30 April 1976 (1976-04-30) * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111110863A (en) * | 2020-01-21 | 2020-05-08 | 郑州大学 | Rhein phospholipid complex, preparation method and application thereof, rhein phospholipid complex long-circulating liposome and preparation method thereof |
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