CN102329853A - Method for determining isolated soy protein digestibility in vitro - Google Patents
Method for determining isolated soy protein digestibility in vitro Download PDFInfo
- Publication number
- CN102329853A CN102329853A CN2010102274510A CN201010227451A CN102329853A CN 102329853 A CN102329853 A CN 102329853A CN 2010102274510 A CN2010102274510 A CN 2010102274510A CN 201010227451 A CN201010227451 A CN 201010227451A CN 102329853 A CN102329853 A CN 102329853A
- Authority
- CN
- China
- Prior art keywords
- sample
- soybean protein
- protein isolate
- described method
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention provides a method for determining isolated soy protein digestibility in vitro. The method includes the following steps: preparing the aqueous solution of isolated soy protein as a sample; using one or more varieties of enzymes to simulate the environment of the human gastrointestinal tract to digest the sample in vitro; stopping the process of digestion, and obtaining a sample to be determined; and determining the in-vitro digestibility of the sample to be determined. The method has the advantages of energy saving, environment-friendliness, safety, high efficiency and the like, and is easy to operate.
Description
Technical field
The present invention relates to foods processing technique, relate more specifically to be used for the method for external test soybean protein isolate digestibility.
Background technology
Soybean protein isolate is to be a kind of full price protein food of raw material production with the low temperature desolventizing soybean meal.Protein contnt is more than 90%, and the amino acid kind has nearly 20 kinds, and contains essential amino acid.It is nutritious; Do not contain SUV, research in recent years confirms that Sunlover 10 has the health care value that meat proteins does not have; Be one of kind of alternative animal proteinum few in number in the vegetable-protein, play an important role with the health that guarantees people maintaining the human life.But the Sunlover 10 molecular structure is tight, and enzymolysis is had very strong resistibility, is unfavorable for intravital digestion of people and absorption, and its physiologically active and digestibility are the main difficult problems of exploitation Sunlover 10 processing technology.Therefore, measuring albumen has important practical significance to improving and improve a Sunlover 10 processing difficult problem in the intravital digestibility of people.
At present proteopeptic research method is mainly contained digestion method in chemical method analysis, the body (human body and experimentation on animals method) and in-vitro simulated method etc.Chemical analysis is fast and convenient, but there is very big-difference in this utilization between measured albumen nutrient of summary nutrient analysis and human consumption absorption nutrient, is not enough to reflect exactly proteic actual nutritive value.Digestion method (human body and experimentation on animals method) is to measure protein digestion the best way in the body, but this method is complicated, the time is long, expense is high; And owing to the several factors influence is difficult to accomplish.Compare with preceding two kinds of methods, advantages such as that in-vitro simulated method has is quick, easy, good reproducibility, though digestive process is difficult to accurate reproduction in the body, still, being present in intravital condition can be at reproducing in vitro.Through simulation to truth in the body, can reflect well that food digests and assimilates in vivo, can evaluate a plurality of samples at short notice.
Existing in the prior art document about Sunlover 10 being carried out the record that the external digestion rate is measured.For example; (the comparisons of some protein raw material external digestion rate measuring method such as yellow the deep blue sea; Fodder industry; Chinese Journal Of High Pressure Physics, 2005 the 26th the 20th phases of volume) adopt stomach en--trypsinase two-step approach, the thick zyme extract digestion method of predacious fish (like perch) digestive tube and the thick zyme extract digestion method of herbivorous fishes (like grass carp) digestive tube to measure the digestibility of 7 kinds of protein raw materials such as casein, fish meal, dregs of beans, rapeseed meal, yeast powder and Zein powder respectively.The result shows that stomach en--trypsinase two-step approach can substitute the thick enzyme digestion of fish Digestive system when measuring fish meal protein matter digestibility, should be prudent for other this method of protein raw material use.Pedersen; B. and Eggum; B.O. (Prediction ofprotein digestibility by an in vitro enzymatic pH-stat procedure; The 49th phase of nineteen eighty-three) measured the digestibility of 61 kinds of food proteinss with in vitro method, testing (rat test) in the mixture of 57 kind of plant albumen or vegetable-protein and animal proteinum wherein, the digestibility that experiment in vitro is surveyed and body has good dependency.(the relation research of the outer digestibility of dregs of beans grinding particle size and aleuroplast such as Li Qingxiao; The ecology of domestic animals newspaper; 2005 the 26th the 5th phases of volume) adopt stomach en-one pancreatin two-step approach; Come the proteopepsis rate of comparison difference grinding particle size dregs of beans through environment in the in-vitro simulated chicken body digestive tube, thereby confirm to be fit to the suitable dregs of beans grinding particle size of broiler growth.
Protein digestion in vivo is one two step process, and to be protein contact with the stomach en-of digestibility in advance the first step, and second step, to be protein contacted digestion with amino acid with tryptic.At present, stomach en--pancreatin two step external digestion methods are a kind of methods of measuring protein digestibility preferably of generally acknowledging.But this method is used for the digestibility of feed mostly to be measured; Though research relevant for the Sunlover 10 external digestion; But the proteolytic enzyme that is adopted in these researchs does not pass through the stdn of enzyme; The activity change scope of enzyme may be bigger in external digestion, and not high to the simulation and the fidelity of condition in the body.Like this, can make interior measure of digestibility unstable result of body and unmatchful ratio with reference to property.Therefore, also need improve to improve its stability of measuring the result and to contrast existing Sunlover 10 external digestion method with reference to property.
Summary of the invention
To the deficiency that prior art exists, technical problem to be solved by this invention is to provide a kind of method that is used for external test soybean protein isolate digestibility.
The invention reside in the measuring method that a kind of soybean protein isolate external digestion rate is provided, this method is on the prior art basis, to improve, and can improve the stability of measuring the result.
According to the present invention, the said method that is used for external test soybean protein isolate digestibility comprises the following steps:
The aqueous solution of preparation soybean protein isolate (0.01～0.1%, g/100mL), as sample;
Use one or more enzyme imitations human gastrointestinal tract's environment that said sample is carried out external digestion;
Stop digestive process, obtain testing sample; And
Measure the external digestion rate of said testing sample.
In a preferred embodiments, before the aqueous solution of preparation soybean protein isolate, can at first carry out stdn and detect said soybean protein isolate.
In a preferred embodiments, the protein contnt of said soybean protein isolate is preferably more than 90% (butt).
In a preferred embodiments, said water is preferably distilled water.
In a preferred embodiments, said enzyme can be selected from stomach en-, pancreatin, trypsinase and Quimotrase.
In a preferred embodiments, the process that digests said sample can may further comprise the steps:
The pH that regulates said sample is to about 1.5;
Add stomach en-;
The said sample of incubation;
Improve pH to about 7.8;
Add trypsinase; And
The said sample of incubation.
In a preferred embodiments, the step that stops digestive process can comprise adding Na
2CO
3Solution is in said testing sample, and existing side by side is about to said testing sample and puts into ice bath and cool off.
In a preferred embodiments, can adopt trichoroacetic acid(TCA)-soluble nitrogen method to carry out the external digestion rate and measure.
Compare with measuring method of the prior art, the method that is used for external test soybean protein isolate digestibility of the present invention has the following advantages:
1, the method for the present invention's employing, simulation food digestion condition in vivo, the human consumption environment of trying one's best, safety and environmental protection, technological operation is simple and convenient, is easy to control and management.
2, the method that adopts of the present invention has been avoided because the mensuration difference that enzyme class, vigor difference cause,
3, the method for the present invention's employing to grasping soybean protein isolate external digestion rate, is set up digestion model in the soybean separation protein lean type, and direct and important reference frame can be provided.
Understand essence of the present invention for better,,, specify but do not limit the present invention through description to preferred implementation of the present invention below in conjunction with accompanying drawing.
Description of drawings
Fig. 1 is the schema of external test soybean protein isolate digestibility of the present invention.
Embodiment
The flow process of measuring method according to the invention is as shown in Figure 1.
In an embodiment of the present invention, the method that is used for external test soybean protein isolate digestibility provided by the present invention can comprise the following steps:
1) aqueous solution of preparation soybean protein isolate is as sample;
2) use the environment of one or more enzyme simulation human gastrointestinal tracts that said sample is carried out external digestion;
3) stop digestive process, obtain testing sample; And
4) the external digestion rate of the said testing sample of mensuration.
The method that is used for external test soybean protein isolate digestibility of the present invention is through the intravital digestive environments of anthropomorphic dummy (pH and temperature etc.); With the gastrointestinal protein enzyme soybean protein isolate is carried out the two step external digestions of stomach en--pancreatin; The time simulation food of external digestion is through the needed time of digestive tube of human body; And adopt trichoroacetic acid(TCA)-soluble nitrogen method to carry out the mensuration of proteopepsis rate, thereby can be for the nutritive value and the method for assess proteins feed are given information exactly.This method can be used for the mensuration of various soybean protein isolates (comprising the sex change soybean protein isolate that obtains through thermal treatment or autoclaving) external digestion rate prevailingly.
In step 2) in, said enzyme can be selected from stomach en-, pancreatin, trypsinase and Quimotrase etc.Used enzyme is preferably the stdn enzyme, and the for example stomach en-of sigma company and trypsinase are to improve circulation ratio and the comparability of measuring the result.The enzyme that is used for external digestion of the present invention is produced according to USP standard, and the active height of enzyme is consistent.USP USP is a kind of NGO non governmental organization, and it guarantees that through setting up the state of the art standard drug quality and other healthcare technology are to promote publilc health.These standards are able to develop and accepted by the world through the process of public participation.
In a preferred embodiments, said step 2) may further comprise the steps:
A) regulate pH to about 1.5;
B) add stomach en-;
C) incubation sample;
D) improve pH to about 7.8;
E) add trypsinase;
F) incubation sample.
In the present invention, the step of termination digestive process can comprise adding Na
2CO
3Solution is in sample, and the sample of existing side by side is soon put into ice bath and cooled off.
Before the aqueous solution of preparation soybean protein isolate, at first said soybean protein isolate is carried out stdn usually and detect.Examination criteria can be: the protein contnt of said soybean protein isolate generally should be more than 90% (butt), and impurity is few, and moisture content is about 5%.Soybean protein isolate stdn of the present invention detects; According to national standard (in the GB/T 5009.5-2003 food in protein measuring, the GB/T5009.3-2003 food in the mensuration of moisture, the GB/T 5009.6-2003 food in mensuration and the GB/T5009.4-2003 food of fat the mensuration of ash content) requirement; Carry out the check of the albumen, moisture, fat, ash etc. of raw material; Guarantee that raw material meets the requirement of the few impurity of protein-high, detection method simple operations.
Wherein, the used water of obtained aqueous solution can be zero(ppm) water or distilled water.In a preferred embodiments of the present invention, said water is preferably distilled water.Zero(ppm) water is the pure water that does not contain any mineral element and impurity, and distilled water is carrying out single vaporization to zero(ppm) water exactly, and purity is higher.Because of a certain amount of impurity can produce certain influence to the uhp treatment sample, so preferred distilled water.
In an embodiment, the process that digests said sample can may further comprise the steps:
The pH that regulates said sample is to about 1.5;
Add stomach en-;
The said sample of incubation;
Improve pH to about 7.8;
Add trypsinase; And
The said sample of incubation.
In an embodiment, the step that stops digestive process can comprise adding Na
2CO
3Solution is in said testing sample, and existing side by side is about to said testing sample and puts into ice bath and cool off.
Wherein, can adopt trichoroacetic acid(TCA)-soluble nitrogen method to carry out the external digestion rate measures.Trichoroacetic acid(TCA) of the present invention-soluble nitrogen method is to be insoluble in the trichoroacetic acid(TCA) solution according to macro-molecular protein, has only small-molecule peptide and total free aminoacids just to be dissolved in principle wherein.This is the method for difference macro-molecular protein with its hydrolysate peptide, the total free aminoacids of classics.
For example, in a preferred embodiments, measuring method of the present invention can comprise the following steps:
1) stdn of soybean isolate protein powder detects
2) liquid stock preparation
Selecting the soybean protein isolate (SPI) that contains more than the protein 90% (butt) for use is raw material, and using distilled water obtain solution, concentration down at 20～25 ℃ is 0.1%～1%.
3) protein enzyme solution preparation
Said proteolytic enzyme is stomach en-and trypsinase.Use 0.01M HCl solution to be made into concentration and be the 5mg/mL pepsin solution, be made into the trypsin solution of 5mg/mL with pH 7.0 PBSs.Two kinds of protein enzyme solutions must fresh.
4) external digestion
The simulation soybean protein isolate adopts stomach en--trypsinase two-step approach to carry out external digestion at human gastrointestinal tract's digestive process.Be deployed into 0.1%～1.0% soy bean proteinous soln with distilled water, regulate pH to 1.5.Sample is put into 37 ℃ of constant-temperature shaking shaking tables, add pepsin solution, make its consumption reach 1%～5%, begin digestion.Behind the 30min, transfer pH to 6.0 stopped reactions.Elevated temperature to 40 ℃ subsequently, transferring pH is 7.8.Add fresh trypsin solution 1%～5%, continue digestion 60min.
5) stop digestion
The method that stops digestion comprises adding Na
2CO
3Solution is in the sample that is reacting, and the digest of existing side by side is soon put into ice bath and cooled off.
6) external digestion rate is measured
Adopt trichoroacetic acid(TCA)-soluble nitrogen (TCA-NSI) method that the external digestion rate of sample is measured.Step is following: the different Digestive systems of 10mL are added the 10%TCA of 10mL, behind the centrifugal 30min of 5000 * g, pour out supernatant.The deposition part is washed with the 10%TCA of 10mL again, and centrifugal under similarity condition, obtains the TCA indissolvable component.The insoluble nitrogen content of protein total nitrogen and TCA adopts nitrogen determination to record.Digestive process nitrogen burst size (%) is got by computes:
In the formula: N
tThe insoluble nitrogen of TCA during-digestion t min, mg
N
0The insoluble nitrogen of TCA among the-SPI, mg
N
TotThe total nitrogen of-SPI, mg
Compared with prior art, the method that is used for external test soybean protein isolate digestibility of the present invention has the following advantages:
1, the method for the present invention's employing, simulation food digestion condition in vivo, the human consumption environment of trying one's best, safety and environmental protection, technological operation is simple and convenient, is easy to control and management.
2, the method that adopts of the present invention has been avoided because the mensuration difference that enzyme class, vigor difference cause,
3, the method for the present invention's employing to grasping soybean protein isolate external digestion rate, is set up digestion model in the soybean separation protein lean type, and direct and important reference frame can be provided.
The used experiment material of the present invention like no specified otherwise, is the commercially available prod.
Embodiment 1
Get brand A, B soybean protein isolate sample respectively, be deployed into 0.3% soy bean proteinous soln, transfer pH to 1.5 with distilled water.Sample is put into 37 ℃ of constant-temperature shaking shaking tables, and (sigma company, P6887) solution make its consumption reach 1%, begin digestion to add stomach en-.Behind the 30min, transfer pH to 6.0 stopped reactions.Elevated temperature to 40 ℃ subsequently, transferring pH is 7.8.Add fresh trypsin sigma company, P1625) solution makes its consumption reach 3%, after continuing to digest 60min, adds a certain amount of Na
2CO
3Solution is in digest, and the digest of existing side by side is soon put into ice bath and cooled off termination reaction.Adopting trichoroacetic acid(TCA)-soluble nitrogen method that the Sunlover 10 sample digestion is carried out the external digestion rate measures.Each sample repeats 6 times.
Embodiment 2 (comparative example)
Take by weighing brand A, each 1.00g of B soybean protein isolate sample respectively, add the pepsin solution of the 0.01g/mL (the pH value is 1.5) of 10mL, seal with para, hydrolysis 4h transfers to neutrality with NaOH in 35 ℃ shaking table.When trypsin treatment; Earlier ready dialysis tubing one end is tightened to no water clock with nylon wire. the front is transferred in the dialysis tubing through neutral sample full dose (uses the 40mL phosphate buffer solution approximately) again; The trypsin solution that adds 10mL 0.025g/mL (the pH value is 7.6); The other end is also tightened to no water clock with nylon wire, places the wide-mouth Erlenmeyer flask of 1000mL.Add the phosphate buffer solution of 300mL dialyzate, seal with para. on shaking table, be hydrolyzed into 24h.Then the interior resistates of dialyzate and pipe is done quantitative analysis.Each sample repeats 6 times.
Digestibility (%)=(N
d/ N
s) * 100%
In the formula: N
dProtein content in the-dialyzate
N
sProtein content in the-sample
The digestibility of three kinds of measured soybean protein isolate products of embodiment is as shown in table 1.
Table 1 different measuring methods gained soybean protein isolate external digestion rate (n=6)
Annotate: a, b, c, d are in same column data, and the identical difference of then representing is not remarkable, and difference is then represented significant difference (p＜0.05)
Visible by table 1 gained data, embodiment 1 and 2 gained data are comparatively approaching.But embodiment 1 records the mv of soybean protein isolate external digestion rate and is merely 0.62 and 0.78.Repeatability better.And embodiment 2 measured data mvs all reach more than 1.5.Repeatability is relatively poor relatively.Simultaneously, embodiment 2 is consuming time longer, is unfavorable for accurately measuring with repeatability of Sunlover 10 external digestion.Can find out that proteinic digestibility determination data vary stable, good reproducibility are simple to operate among the embodiment 1, and the protein digestibility significant difference between each sample.Therefore, this method can be used for measuring the external digestion rate of soybean protein isolate.
More than the description of preferred embodiments of the present invention is not limited the present invention, those skilled in the art can make various changes or distortion according to the present invention, only otherwise break away from spirit of the present invention, all should belong to the scope of accompanying claims of the present invention.
Claims (9)
1. method that is used for external test soybean protein isolate digestibility, this method comprises the following steps:
The aqueous solution of preparation soybean protein isolate is as sample;
Use the environment of one or more enzyme simulation human gastrointestinal tracts that said sample is carried out external digestion;
Stop digestive process, obtain testing sample; And
Measure the external digestion rate of said testing sample.
2. the described method of claim 1 wherein, before the aqueous solution of preparation soybean protein isolate, is carried out stdn to said soybean protein isolate and is detected.
3. the described method of claim 2, wherein, the protein contnt of said soybean protein isolate is more than 90% (butt).
4. any described method in the claim 1,2 and 3, wherein, said water is distilled water.
5. any described method in the claim 1,2,3 and 4, wherein, said enzyme is selected from stomach en-, pancreatin, trypsinase and Quimotrase.
6. the described method of claim 5, wherein, said enzyme is the stdn enzyme.
7. any described method in the claim 1,2,3,4,5 and 6, wherein, the process of the said sample of external digestion may further comprise the steps:
The pH that regulates said sample is to about 1.5;
Add stomach en-;
The said sample of incubation;
Improve pH to about 7.8;
Add trypsinase; And
The said sample of incubation.
8. any described method in the claim 1,2,3,4,5,6 and 7, wherein, the step that stops digestive process comprises and adds Na
2CO
3Solution is in said testing sample, and existing side by side is about to said testing sample and puts into ice bath and cool off.
9. any described method in the claim 1,2,3,4,5,6,7 and 8 wherein, adopts trichoroacetic acid(TCA)-soluble nitrogen method to carry out the external digestion rate and measures.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102274510A CN102329853A (en) | 2010-07-12 | 2010-07-12 | Method for determining isolated soy protein digestibility in vitro |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102274510A CN102329853A (en) | 2010-07-12 | 2010-07-12 | Method for determining isolated soy protein digestibility in vitro |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102329853A true CN102329853A (en) | 2012-01-25 |
Family
ID=45481815
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010102274510A Pending CN102329853A (en) | 2010-07-12 | 2010-07-12 | Method for determining isolated soy protein digestibility in vitro |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102329853A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102928581A (en) * | 2012-11-12 | 2013-02-13 | 中国农业科学院北京畜牧兽医研究所 | External measurement method for transgenic plant material digestibility |
| CN104263809A (en) * | 2014-08-27 | 2015-01-07 | 山东农业大学 | In-vitro enzyme digestion method for evaluating amino acid content of soybean meal for poultry |
| CN107739748A (en) * | 2017-10-12 | 2018-02-27 | 广州傲农生物科技有限公司 | A kind of assay method of Pepsin digestibility and its application |
| CN108226407A (en) * | 2018-01-02 | 2018-06-29 | 扬州大学 | A kind of method of quick detection rice digestion characteristics |
| US10191062B2 (en) | 2013-11-25 | 2019-01-29 | University Of Newcastle Upon Tyne | Model gut system |
| CN113702568A (en) * | 2021-08-06 | 2021-11-26 | 福建傲农生物科技集团股份有限公司 | Method for measuring pepsin digestibility of animal protein feed |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009088931A1 (en) * | 2008-01-03 | 2009-07-16 | Monsanto Technology Llc | Method of selecting soybeans with enhanced bioactivity and compositions for reducing cancer cell viability |
| CN101664100A (en) * | 2009-08-24 | 2010-03-10 | 天津春发食品配料有限公司 | Modified and solubilized soybean protein isolate |
-
2010
- 2010-07-12 CN CN2010102274510A patent/CN102329853A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009088931A1 (en) * | 2008-01-03 | 2009-07-16 | Monsanto Technology Llc | Method of selecting soybeans with enhanced bioactivity and compositions for reducing cancer cell viability |
| CN101664100A (en) * | 2009-08-24 | 2010-03-10 | 天津春发食品配料有限公司 | Modified and solubilized soybean protein isolate |
Non-Patent Citations (5)
| Title |
|---|
| SU DAN ET AL.: "Effects of high hydrostatic pressure on in vitro digestion of soy protein", 《INTERNATIONAL AGRICULTURAL ENGINEERING JOURNAL》 * |
| SYLVIE F. GAUTHIER ET AL.: "Assessment of Protein Digestibility by In Vitro Enzymatic Hydrolysis with Simultaneous Dialysis", 《THE JOURNAL OF NUTRITION》 * |
| 于立梅等: "野生山毛豆蛋白制备及体外消化模拟研究", 《食品科学》 * |
| 周志红等: "大豆蛋白的体外模拟消化过程及热处理的影响", 《食品科学》 * |
| 苏丹等: "中心组合设计优化超高压处理的大豆蛋白体外消化工艺", 《农产品加工学刊》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102928581A (en) * | 2012-11-12 | 2013-02-13 | 中国农业科学院北京畜牧兽医研究所 | External measurement method for transgenic plant material digestibility |
| CN102928581B (en) * | 2012-11-12 | 2014-08-06 | 孙哲 | External measurement method for transgenic plant material digestibility |
| US10191062B2 (en) | 2013-11-25 | 2019-01-29 | University Of Newcastle Upon Tyne | Model gut system |
| CN104263809A (en) * | 2014-08-27 | 2015-01-07 | 山东农业大学 | In-vitro enzyme digestion method for evaluating amino acid content of soybean meal for poultry |
| CN104263809B (en) * | 2014-08-27 | 2015-11-11 | 山东农业大学 | A kind of method by vitro enzyme digestion method evaluation dregs of beans poultry Available Amino Acids content |
| CN107739748A (en) * | 2017-10-12 | 2018-02-27 | 广州傲农生物科技有限公司 | A kind of assay method of Pepsin digestibility and its application |
| CN108226407A (en) * | 2018-01-02 | 2018-06-29 | 扬州大学 | A kind of method of quick detection rice digestion characteristics |
| CN113702568A (en) * | 2021-08-06 | 2021-11-26 | 福建傲农生物科技集团股份有限公司 | Method for measuring pepsin digestibility of animal protein feed |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Stern et al. | Methods for determining and factors affecting rumen microbial protein synthesis: a review | |
| Morris et al. | Immunostimulant activity of an enzymatic protein hydrolysate from green microalga Chlorella vulgaris on undernourished mice | |
| Atkinson et al. | Human milk: comparison of the nitrogen composition in milk from mothers of premature and full-term infants | |
| CN102329853A (en) | Method for determining isolated soy protein digestibility in vitro | |
| US9125851B2 (en) | Active small-molecule donkey-hide gelatin mixture and preparation method and application thereof | |
| Strong et al. | Microbiological assay for pantothenic acid | |
| Uys et al. | Changes in digestive enzyme activities of Clarias gariepinus (Pisces: Clariidae) after feeding | |
| CN113088548A (en) | Preparation method of oyster antioxidant active peptide | |
| CN110195090A (en) | The preparation method and gained sea cucumber intestine ovum polypeptide powder of sea cucumber intestine ovum polypeptide powder | |
| Acharya et al. | Response of lactating dairy cows to dietary protein from canola meal or distillers’ grains on dry matter intake, milk production, milk composition, and amino acid status | |
| Chen et al. | Beneficial effect of Rhodopseudomonas palustris on in vitro rumen digestion and fermentation | |
| Hamdan et al. | Development of an in vitro model to assess protein bioavailability in diets for common octopus (Octopus vulgaris) | |
| CN102827910A (en) | Preparation method and application of small peptide chelated copper compound | |
| Qiu et al. | Fermented feed modulates meat quality and promotes the growth of longissimus thoracis of late-finishing pigs | |
| Selle et al. | The impact of digestive dynamics on the bioequivalence of amino acids in broiler chickens | |
| Voss et al. | Processed by-products from soy beverage (Okara) as sustainable ingredients for nile tilapia (O. niloticus) juveniles: Effects on nutrient utilization and muscle quality | |
| Sheng et al. | Incorporation of 15NH4Cl into histidine in adult man | |
| CN106222225B (en) | A kind of preparation method of ox bone active peptide | |
| Qiu et al. | Effects of dietary crude protein and protease levels on performance, immunity capacity, and AA digestibility of broilers | |
| Santos et al. | The influence of casein and urea as nitrogen sources on in vitro equine caecal fermentation | |
| Wang et al. | Comparative study on the residual rate of immunoreactive soybean glycinin (11S) in the digestive tract of pigs of different ages | |
| CN104711217A (en) | Novel bioactive peptide for promoting lactic acid bacteria growth | |
| CN102827908A (en) | Preparation method and application of small peptide chelated iron compound | |
| CN110093333A (en) | A kind of application in compound protease and its low-protein diet | |
| CN109674040A (en) | Production method of high activity yolk fermentation material and products thereof and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120125 |