CN102329836A - Method for producing 5'-adenosine by adopting bacillus - Google Patents
Method for producing 5'-adenosine by adopting bacillus Download PDFInfo
- Publication number
- CN102329836A CN102329836A CN201110277143A CN201110277143A CN102329836A CN 102329836 A CN102329836 A CN 102329836A CN 201110277143 A CN201110277143 A CN 201110277143A CN 201110277143 A CN201110277143 A CN 201110277143A CN 102329836 A CN102329836 A CN 102329836A
- Authority
- CN
- China
- Prior art keywords
- fermented liquid
- adenosine
- genus bacillus
- hours
- enzyme reagent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to a method for producing 5'-adenosine by adopting bacillus, and the method comprises the following specific steps: (1) using a bacillus culture medium to culture the bacillus; (2) using a fermentation culture medium to culture fermentation broth; (3) putting adenosine into the fermentation broth when the bacterial concentration of the bacillus achieves 28%-35%, controlling the temperature of the fermentation broth at 25-40 DEG C, fermenting for 20-25 hours and then adding an enzyme reagent based on the condition that the ratio of the adenosine to the enzyme reagent is (95:1)-(105:1); and (4) squeezing the fermentation broth after 8-16 hours, loading the fermentation broth to a resin column, eluting for getting a clear solution and regulating the pH value of the clear solution until the clear solution is acid so as to get the 5'-adenosine. The yield of the 5'-adenosine product obtained by the method is higher.
Description
Technical field
The present invention relates to the medicine intermediate technical field, the method that especially a kind of employing genus bacillus produces 5 '-adenylic acid(AMP).
Background technology
In the prior art, the conventional employing has the common micro-organisms fermentation process, and raw material availability is low only to have 61.2%, and the yield of product is merely 1.05; Perhaps, adopt chemical synthesis process, still; Adopt the used part material of chemical synthesis process to belong to hazardous and noxious substances such as trihalophosporus oxide; Make to be prone in process of production human body is produced injury, and product can only be used for biochemical reagents production, can not be applied to food service industry.Puerarin (HPLC) content is about 97.5% in 5 '-adenylic acid(AMP) product that conventional production methods is produced, and ultraviolet content (UV) is about 97%.
Summary of the invention
The technical problem that the present invention will solve is: overcome the deficiency of prior art, and the method that provides a kind of employing genus bacillus to produce 5 '-adenylic acid(AMP), the yield of 5 '-adenylic acid(AMP) that this method is produced is higher.
The technical solution adopted for the present invention to solve the technical problems is: the method that a kind of employing genus bacillus produces 5 '-adenylic acid(AMP); Be specially: genus bacillus is cultivated with bacillus culture medium in (1); (2) with fermention medium cultivation and fermentation liquid, drop into adenosine in the fermented liquid when reaching 28%～35% when the bacterium of genus bacillus is dense (3), and the fermented liquid temperature is controlled at 25～40 degrees centigrade; Ferment after 20～25 hours, add enzyme reagent according to adenosine and 95: 1～105: 1 ratio of enzyme reagent; Squeeze fermented liquid after (4) 8～16 hours, with resin column on the fermented liquid, get clear liquid through wash-out, regulating the clear liquid pH value is to obtain 5 '-adenylic acid(AMP) after the acidity.
Particularly, described bacillus culture medium comprises: peptone 20g/L, beef extract powder 5g/L, sodium-chlor 5g/L.
Particularly, described fermention medium comprises: glucose 20g/L, yeast extract 4.0g/L, lime carbonate 5g/L, ammonium sulfate 10g/L, potassium primary phosphate 1.0g/L, iron vitriol 0.01g/L, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.01g/L.
Particularly; Step (3) step (4) is specially: drop into adenosine in the fermented liquid when reaching 30% when the bacterium of genus bacillus is dense (3); The fermented liquid temperature is controlled at 28～35 degrees centigrade, ferments after 22～24 hours, adds enzyme reagent according to adenosine and 100: 1 ratio of enzyme reagent; Squeeze fermented liquid after (4) 12 hours, with resin column on the fermented liquid, get clear liquid through wash-out, regulating the clear liquid pH value is to obtain 5 '-adenylic acid(AMP) after the acidity.
Particularly; Step (3) step (4) is specially: drop into adenosine in the fermented liquid when reaching 30.5% when the bacterium of genus bacillus is dense (3); The fermented liquid temperature is controlled at 27～34 degrees centigrade, ferments after 21～23 hours, adds enzyme reagent according to adenosine and 101: 1 ratio of enzyme reagent; Squeeze fermented liquid after (4) 11～12 hours, with resin column on the fermented liquid, get clear liquid through wash-out, regulating the clear liquid pH value is to obtain 5 '-adenylic acid(AMP) after the acidity.
Particularly, step (3) step (4) is specially: drop into the 500g adenosine in the 3L fermented liquid when the dense 30.5%-32% of reaching of the bacterium of genus bacillus (3); The fermented liquid temperature is controlled at 28～35 degrees centigrade, ferments after 22～24 hours, after 0.4～0.5 hour, acetylphosphate is added in the fermented liquid as enzyme reagent, adds enzyme reagent according to adenosine and 100: 1 ratio of enzyme reagent; Squeeze fermented liquid after (4) 12 hours, with resin column on the fermented liquid, get clear liquid through wash-out, regulating the clear liquid pH value is to obtain 5 '-adenylic acid(AMP) after the acidity.
A kind of method of utilizing genus bacillus to produce 5 '-adenylic acid(AMP), genus bacillus is cultivated with bacillus culture medium in (1), and described bacillus culture medium comprises: peptone 20g/L, beef extract powder 5g/L, sodium-chlor 5g/L; (2) with fermention medium cultivation and fermentation liquid, described fermention medium comprises: glucose 20g/L, yeast extract 4.0g/L, lime carbonate 5g/L, ammonium sulfate 10g/L, potassium primary phosphate 1.0g/L, iron vitriol 0.01g/L, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.01g/L; (3) adenosine is dropped in the fermented liquid when reaching 28%～35% when the bacterium of genus bacillus is dense, the fermented liquid temperature is controlled at 25～40 degrees centigrade, ferments after 20～25 hours, adds enzyme reagent according to adenosine and 95: 1～105: 1 ratio of enzyme reagent; Squeeze fermented liquid after (4) 8～16 hours, with resin column on the fermented liquid, get clear liquid through wash-out, regulating the clear liquid pH value is to obtain 5 '-adenylic acid(AMP) after the acidity.
Particularly, step (3) step (4) is specially: drop into the 500g adenosine in the 3L fermented liquid when the dense 30.5%-32% of reaching of the bacterium of genus bacillus (3); The fermented liquid temperature is controlled at 28～35 degrees centigrade, ferments after 22～24 hours, after 0.4～0.5 hour, acetylphosphate is added in the fermented liquid as enzyme reagent, adds enzyme reagent according to adenosine and 100: 1 ratio of enzyme reagent; Squeeze fermented liquid after (4) 12 hours, with resin column on the fermented liquid, get clear liquid through wash-out, regulating the clear liquid pH value is to obtain 5 '-adenylic acid(AMP) after the acidity.
The invention has the beneficial effects as follows: the 5 '-adenylic acid(AMP) that obtains through aforesaid method is 5 '-adenylic acid(AMP) bullion, and 5 '-adenylic acid(AMP) bullion gets 5 '-adenylic acid(AMP) finished product according to the crystallization procedure crystallization.Puerarin (HPLC) content through check 5 '-adenylic acid(AMP) product is 99%; Ultraviolet content (UV) content is 98.5%; The yield of 5 '-adenylic acid(AMP) product is 1.25, than common micro-organisms fermentation process used in the prior art and chemical synthesis process the yield of product all exceed a lot.
Embodiment
Embodiment one:
The method that a kind of employing genus bacillus produces 5 '-adenylic acid(AMP); Be specially: genus bacillus is cultivated with bacillus culture medium in (1); (2) with fermention medium cultivation and fermentation liquid, drop into adenosine in the fermented liquid when reaching 28%～35% when the bacterium of genus bacillus is dense (3), and the fermented liquid temperature is controlled at 25～40 degrees centigrade; Ferment after 20～25 hours, add enzyme reagent according to adenosine and 95: 1～105: 1 ratio of enzyme reagent; Squeeze fermented liquid after (4) 8～16 hours, with resin column on the fermented liquid, get clear liquid through wash-out, regulating the clear liquid pH value is to obtain 5 '-adenylic acid(AMP) after the acidity.
Embodiment two:
The method that a kind of employing genus bacillus produces 5 '-adenylic acid(AMP); Be specially: genus bacillus is cultivated with bacillus culture medium in (1); Bacillus culture medium comprises: peptone 20g/L, beef extract powder 5g/L, sodium-chlor 5g/L; (2) with fermention medium cultivation and fermentation liquid, drop into adenosine in the fermented liquid when reaching 28%～35% when the bacterium of genus bacillus is dense (3), and the fermented liquid temperature is controlled at 25～40 degrees centigrade; Ferment after 20～25 hours, add enzyme reagent according to adenosine and 95: 1～105: 1 ratio of enzyme reagent; Squeeze fermented liquid after (4) 8～16 hours, with resin column on the fermented liquid, get clear liquid through wash-out, regulating the clear liquid pH value is to obtain 5 '-adenylic acid(AMP) after the acidity.
Embodiment three:
The method that a kind of employing genus bacillus produces 5 '-adenylic acid(AMP); Be specially: genus bacillus is cultivated with bacillus culture medium in (1); (2) with fermention medium cultivation and fermentation liquid; Fermention medium comprises: glucose 20g/L, yeast extract 4.0g/L, lime carbonate 5g/L, ammonium sulfate 10g/L, potassium primary phosphate 1.0g/L, iron vitriol 0.01g/L, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.01g/L, and drop into adenosine in the fermented liquid when reaching 28%～35% when the bacterium of genus bacillus is dense (3), and the fermented liquid temperature is controlled at 25～40 degrees centigrade; Ferment after 20～25 hours, add enzyme reagent according to adenosine and 95: 1～105: 1 ratio of enzyme reagent; Squeeze fermented liquid after (4) 8～16 hours, with resin column on the fermented liquid, get clear liquid through wash-out, regulating the clear liquid pH value is to obtain 5 '-adenylic acid(AMP) after the acidity.
Embodiment four:
The method that a kind of employing genus bacillus produces 5 '-adenylic acid(AMP); Be specially: genus bacillus is cultivated with bacillus culture medium in (1); (2) with fermention medium cultivation and fermentation liquid, drop into adenosine in the fermented liquid when reaching 30% when the bacterium of genus bacillus is dense (3), and the fermented liquid temperature is controlled at 28～35 degrees centigrade; Ferment after 22～24 hours, add enzyme reagent according to adenosine and 100: 1 ratio of enzyme reagent; Squeeze fermented liquid after (4) 12 hours, with resin column on the fermented liquid, get clear liquid through wash-out, regulating the clear liquid pH value is to obtain 5 '-adenylic acid(AMP) after the acidity.
Embodiment five:
The method that a kind of employing genus bacillus produces 5 '-adenylic acid(AMP); Be specially: genus bacillus is cultivated with bacillus culture medium in (1); (2) with fermention medium cultivation and fermentation liquid, drop into adenosine in the fermented liquid when reaching 30.5% when the bacterium of genus bacillus is dense (3), and the fermented liquid temperature is controlled at 27～34 degrees centigrade; Ferment after 21～23 hours, add enzyme reagent according to adenosine and 101: 1 ratio of enzyme reagent; Squeeze fermented liquid after (4) 11～12 hours, with resin column on the fermented liquid, get clear liquid through wash-out, regulating the clear liquid pH value is to obtain 5 '-adenylic acid(AMP) after the acidity.
Embodiment six:
The method that a kind of employing genus bacillus produces 5 '-adenylic acid(AMP); Be specially: genus bacillus is cultivated with bacillus culture medium in (1); (2) with fermention medium cultivation and fermentation liquid, drop into the 500g adenosine in the 3L fermented liquid when the bacterium of genus bacillus is dense when reaching 30.5%-32% (3); The fermented liquid temperature is controlled at 28～35 degrees centigrade, ferments after 22～24 hours, after 0.4～0.5 hour, acetylphosphate is added in the fermented liquid as enzyme reagent, adds enzyme reagent according to adenosine and 100: 1 ratio of enzyme reagent; Squeeze fermented liquid after (4) 12 hours, with resin column on the fermented liquid, get clear liquid through wash-out, regulating the clear liquid pH value is to obtain 5 '-adenylic acid(AMP) after the acidity
Embodiment seven:
A kind of method of utilizing genus bacillus to produce 5 '-adenylic acid(AMP), genus bacillus is cultivated with bacillus culture medium in (1), and described bacillus culture medium comprises: peptone 20g/L, beef extract powder 5g/L, sodium-chlor 5g/L; (2) with fermention medium cultivation and fermentation liquid, described fermention medium comprises: glucose 20g/L, yeast extract 4.0g/L, lime carbonate 5g/L, ammonium sulfate 10g/L, potassium primary phosphate 1.0g/L, iron vitriol 0.01g/L, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.01g/L; (3) adenosine is dropped in the fermented liquid when reaching 28%～35% when the bacterium of genus bacillus is dense, the fermented liquid temperature is controlled at 25～40 degrees centigrade, ferments after 20～25 hours, adds enzyme reagent according to adenosine and 95: 1～105: 1 ratio of enzyme reagent; Squeeze fermented liquid after (4) 8～16 hours, with resin column on the fermented liquid, get clear liquid through wash-out, regulating the clear liquid pH value is to obtain 5 '-adenylic acid(AMP) after the acidity.
Embodiment eight:
A kind of method of utilizing genus bacillus to produce 5 '-adenylic acid(AMP), genus bacillus is cultivated with bacillus culture medium in (1), and described bacillus culture medium comprises: peptone 20g/L, beef extract powder 5g/L, sodium-chlor 5g/L; (2) with fermention medium cultivation and fermentation liquid, described fermention medium comprises: glucose 20g/L, yeast extract 4.0g/L, lime carbonate 5g/L, ammonium sulfate 10g/L, potassium primary phosphate 1.0g/L, iron vitriol 0.01g/L, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.01g/L; (3) when the bacterium of genus bacillus is dense when reaching 30.5%-32%, the 500g adenosine is dropped in the 3L fermented liquid; The fermented liquid temperature is controlled at 28～35 degrees centigrade, ferments after 22～24 hours, after 0.4～0.5 hour, acetylphosphate is added in the fermented liquid as enzyme reagent, adds enzyme reagent according to adenosine and 100: 1 ratio of enzyme reagent; Squeeze fermented liquid after (4) 12 hours, with resin column on the fermented liquid, get clear liquid through wash-out, regulating the clear liquid pH value is to obtain 5 '-adenylic acid(AMP) after the acidity.
The 5 '-adenylic acid(AMP) that obtains through aforesaid method is 5 '-adenylic acid(AMP) bullion, and 5 '-adenylic acid(AMP) bullion gets 5 '-adenylic acid(AMP) finished product 625g according to the crystallization procedure crystallization.Puerarin (HPLC) content through check 5 '-adenylic acid(AMP) product is 99%; Ultraviolet content (UV) content is 98.5%; The yield of 5 '-adenylic acid(AMP) product is 1.25, than common micro-organisms fermentation process used in the prior art and chemical synthesis process the yield of product all exceed a lot.The 5 '-adenylic acid(AMP) product that uses method of the present invention to produce can directly use and food service industry.
Claims (8)
1. method that adopts genus bacillus to produce 5 '-adenylic acid(AMP); Be specially: genus bacillus is cultivated with bacillus culture medium in (1); (2) with fermention medium cultivation and fermentation liquid, it is characterized in that: drop into adenosine in the fermented liquid when reaching 28%～35% when the bacterium of genus bacillus is dense (3), and the fermented liquid temperature is controlled at 25～40 degrees centigrade; Ferment after 20～25 hours, add enzyme reagent according to adenosine and 95: 1～105: 1 ratio of enzyme reagent; Squeeze fermented liquid after (4) 8～16 hours, with resin column on the fermented liquid, get clear liquid through wash-out, regulating the clear liquid pH value is to obtain 5 '-adenylic acid(AMP) after the acidity.
2. the method that employing genus bacillus according to claim 1 produces 5 '-adenylic acid(AMP), it is characterized in that: described bacillus culture medium comprises: peptone 20g/L, beef extract powder 5g/L, sodium-chlor 5g/L.
3. the method that employing genus bacillus according to claim 1 produces 5 '-adenylic acid(AMP), it is characterized in that: described fermention medium comprises: glucose 20g/L, yeast extract 4.0g/L, lime carbonate 5g/L, ammonium sulfate 10g/L, potassium primary phosphate 1.0g/L, iron vitriol 0.01g/L, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.01g/L.
4. the method that employing genus bacillus according to claim 1 produces 5 '-adenylic acid(AMP); It is characterized in that: step (3) step (4) is specially: drop into adenosine in the fermented liquid when reaching 30% when the bacterium of genus bacillus is dense (3); The fermented liquid temperature is controlled at 28～35 degrees centigrade; Ferment after 22～24 hours, add enzyme reagent according to adenosine and 100: 1 ratio of enzyme reagent; Squeeze fermented liquid after (4) 12 hours, with resin column on the fermented liquid, get clear liquid through wash-out, regulating the clear liquid pH value is to obtain 5 '-adenylic acid(AMP) after the acidity.
5. the method that employing genus bacillus according to claim 1 produces 5 '-adenylic acid(AMP); It is characterized in that: step (3) step (4) is specially: drop into adenosine in the fermented liquid when reaching 30.5% when the bacterium of genus bacillus is dense (3); The fermented liquid temperature is controlled at 27～34 degrees centigrade; Ferment after 21～23 hours, add enzyme reagent according to adenosine and 101: 1 ratio of enzyme reagent; Squeeze fermented liquid after (4) 11～12 hours, with resin column on the fermented liquid, get clear liquid through wash-out, regulating the clear liquid pH value is to obtain 5 '-adenylic acid(AMP) after the acidity.
6. employing according to claim 1 genus bacillus produces the method for 5 '-adenylic acid(AMP), and it is characterized in that: step (3) step (4) is specially: drop into the 500g adenosine in the 3L fermented liquid when the dense 30.5%-32% of reaching of the bacterium of genus bacillus (3); The fermented liquid temperature is controlled at 28～35 degrees centigrade, ferments after 22～24 hours, after 0.4～0.5 hour, acetylphosphate is added in the fermented liquid as enzyme reagent, adds enzyme reagent according to adenosine and 100: 1 ratio of enzyme reagent; Squeeze fermented liquid after (4) 12 hours, with resin column on the fermented liquid, get clear liquid through wash-out, regulating the clear liquid pH value is to obtain 5 '-adenylic acid(AMP) after the acidity.
7. method of utilizing genus bacillus to produce 5 '-adenylic acid(AMP), it is characterized in that: genus bacillus is cultivated with bacillus culture medium in (1), and described bacillus culture medium comprises: peptone 20g/L, beef extract powder 5g/L, sodium-chlor 5g/L; (2) with fermention medium cultivation and fermentation liquid, described fermention medium comprises: glucose 20g/L, yeast extract 4.0g/L, lime carbonate 5g/L, ammonium sulfate 10g/L, potassium primary phosphate 1.0g/L, iron vitriol 0.01g/L, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.01g/L; (3) adenosine is dropped in the fermented liquid when reaching 28%～35% when the bacterium of genus bacillus is dense, the fermented liquid temperature is controlled at 25～40 degrees centigrade, ferments after 20～25 hours, adds enzyme reagent according to adenosine and 95: 1～105: 1 ratio of enzyme reagent; Squeeze fermented liquid after (4) 8～16 hours, with resin column on the fermented liquid, get clear liquid through wash-out, regulating the clear liquid pH value is to obtain 5 '-adenylic acid(AMP) after the acidity.
8. the method for utilizing genus bacillus to produce 5 '-adenylic acid(AMP) according to claim 7, it is characterized in that: step (3) step (4) is specially: drop into the 500g adenosine in the 3L fermented liquid when the dense 30.5%-32% of reaching of the bacterium of genus bacillus (3); The fermented liquid temperature is controlled at 28～35 degrees centigrade, ferments after 22～24 hours, after 0.4～0.5 hour, acetylphosphate is added in the fermented liquid as enzyme reagent, adds enzyme reagent according to adenosine and 100: 1 ratio of enzyme reagent; Squeeze fermented liquid after (4) 12 hours, with resin column on the fermented liquid, get clear liquid through wash-out, regulating the clear liquid pH value is to obtain 5 '-adenylic acid(AMP) after the acidity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110277143A CN102329836A (en) | 2011-09-19 | 2011-09-19 | Method for producing 5'-adenosine by adopting bacillus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110277143A CN102329836A (en) | 2011-09-19 | 2011-09-19 | Method for producing 5'-adenosine by adopting bacillus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102329836A true CN102329836A (en) | 2012-01-25 |
Family
ID=45481798
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110277143A Pending CN102329836A (en) | 2011-09-19 | 2011-09-19 | Method for producing 5'-adenosine by adopting bacillus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102329836A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1657608A (en) * | 2005-03-23 | 2005-08-24 | 江苏省微生物研究所有限责任公司 | Adenosme generation bacteria and method for generating adenosine by fermentation |
| EP1700910A2 (en) * | 2005-03-10 | 2006-09-13 | Ajinomoto Co., Inc. | Purine-derived substance-producing Bacillus and a method for producing purine-derived substance therewith |
| WO2007125783A1 (en) * | 2006-04-24 | 2007-11-08 | Ajinomoto Co., Inc. | Bacterium capable of producing purine substance, and process for production of purine substance |
| CN101563448A (en) * | 2006-12-22 | 2009-10-21 | 味之素株式会社 | A method for producing purine nucleosides and nucleotides by fermentation using a bacterium belonging to the genus escherichia or bacillus |
| CN102154165A (en) * | 2011-01-05 | 2011-08-17 | 南京工业大学 | Bacillus subtilis capable of producing adenosine at high yield |
-
2011
- 2011-09-19 CN CN201110277143A patent/CN102329836A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1700910A2 (en) * | 2005-03-10 | 2006-09-13 | Ajinomoto Co., Inc. | Purine-derived substance-producing Bacillus and a method for producing purine-derived substance therewith |
| CN1657608A (en) * | 2005-03-23 | 2005-08-24 | 江苏省微生物研究所有限责任公司 | Adenosme generation bacteria and method for generating adenosine by fermentation |
| WO2007125783A1 (en) * | 2006-04-24 | 2007-11-08 | Ajinomoto Co., Inc. | Bacterium capable of producing purine substance, and process for production of purine substance |
| CN101563448A (en) * | 2006-12-22 | 2009-10-21 | 味之素株式会社 | A method for producing purine nucleosides and nucleotides by fermentation using a bacterium belonging to the genus escherichia or bacillus |
| CN102154165A (en) * | 2011-01-05 | 2011-08-17 | 南京工业大学 | Bacillus subtilis capable of producing adenosine at high yield |
Non-Patent Citations (2)
| Title |
|---|
| TAKAYUKI ASAHARA YUKIKO MORI, ET AL.: "Accumulation of gene-targeted Bacillus subtilis mutations that enhance fermentative inosine production", 《APPL MICROBIOL BIOTECHNOL》, no. 87, 4 June 2010 (2010-06-04) * |
| 王红连等: "枯草芽孢杆菌JSIM-X-13-4突变株腺苷发酵研究", 《食品与发酵工业》, vol. 32, no. 12, 31 December 2006 (2006-12-31), pages 44 - 47 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101418270A (en) | The Lactobacillus casei Zhang high-density cultivation method, use them to prepare the method for freeze-dried vaccine powder and resulting freeze-dried vaccine powder and uses thereof | |
| CN102296102B (en) | Control method for gluconate production by microbiological method | |
| CN105385712B (en) | A kind of method of fermentation productions of ethylformic acid | |
| CN104877886A (en) | Edible vinegar liquid state fermentation nutrient salt formula and edible vinegar liquid state semi-continuous fermentation method | |
| CN104017853A (en) | Method for producing gamma-aminobutyric acid by fermentation | |
| CN102465166A (en) | Method for improving 2-keto-L-gulonic acid fermentation production strength | |
| CN102206684A (en) | Fermentation technology for producing calcium lactate with sweet potatoes as raw material | |
| CN101748177A (en) | Optimized method for producing vitamin B12 through pseuomonas denitrifican fermentation and synthetic medium | |
| CN107354193B (en) | Method for replicating a medium | |
| CN104212851A (en) | Method for producing L-phenylalanine by multistage continuous fermentation | |
| CN101638674B (en) | Method for manufacturing citric acid by utilizing cane sugar hydrolysate fermentation method | |
| CN109182438B (en) | Production of vitamin B by fermentation of bacillus2Culture medium and culture method | |
| CN101638675B (en) | Method for manufacturing citric acid by cane sugar fermentation method | |
| CN102150688A (en) | Prescription of plant lactic acid bacteria solution and preparation technology thereof | |
| CN102329836A (en) | Method for producing 5'-adenosine by adopting bacillus | |
| CN105087417A (en) | Compound microbial agent for aerobic treatment on food processing wastewater and application of compound microbial agent | |
| CN101139553A (en) | Method for producing nutritious yeast powder by monosodium glutamate waste fluid | |
| CN104561141A (en) | Acetic fermentation nutrient and use method thereof | |
| CN101845475B (en) | Nutrition-enhanced culture medium for preparing 2-KGA through fermentation and method thereof for preparing 2-KGA | |
| CN103497988B (en) | Fermentation production method of safe efficient lactobacillus product | |
| CN105331649A (en) | Method for continuous production of gamma-aminobutyric acid | |
| CN101717797B (en) | Method for improving yield of succinic acid by adding key growth factors | |
| CN101555504B (en) | Method for producing D-ribose by utilizing fermentation of bacillus pumilus transketolase variant | |
| CN108936640A (en) | A kind of ferment production method | |
| CN102443610A (en) | Volume dissolved oxygen transfer coefficient staged control method based on citric acid metabolic network |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120125 |