CN102319379A - Formula for treating and preventing cancerous tumors and preparation and production process of Chinese caterpillar fungus tumor rehabilitation oral liquid - Google Patents
Formula for treating and preventing cancerous tumors and preparation and production process of Chinese caterpillar fungus tumor rehabilitation oral liquid Download PDFInfo
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Abstract
The invention provides a formula for treating and preventing cancerous tumors. The formula is characterized in that: a plurality of active ingredients for treating cancerous tumors are mixed. Officinal and edible fungi such as Chinese caterpillar fungus, mycelium of Chinese caterpillar fungus, glossy privet fruit, pawpaw and the like or cancer treating ingredients without toxic or side effect extracted from plants are mixed specifically by selecting relevant ingredients according to disease causes, pathology and development process, so that a mixing unit for preventing cancer gene mutation, killing cancer cells and enhancing the immunity is constituted in a layered way, an effective quantity of cancer cells are killed, and the complexity, intractable property and rapidness of cancers are eliminated respectively. By experimenting, a prescription of which the effective rate and the killing rate on tumor cells and cancer cells is formed. The invention is characterized by further providing a preparation and production process of a Chinese caterpillar fungus tumor rehabilitation oral liquid. Ingredients needed for treating cancers are obtained by alcohol extraction, leaching and membrane separating technologies, and other ingredients are used for treating other diseases, so that the functions and values of raw materials are increased manifold, and the economic benefit is increased greatly.
Description
Technical field
The invention belongs to health food and medical technical field.Be specifically related to from Cordyceps edible funguses such as (Cordyceps Sinensis < Berk>Sacc), screening in food plant such as horse Herba Gynurae bicoloris, Fructus Ligustri Lucidi, papaw leaf or the non-toxic herb, separate, extract and contain treatment and the prevention cancer is brought out, oncogene mutation, growth of cancer cells propagation, tumor form, the effective ingredient of cancerometastasis.Through the science compatibility; Form brand-new, different with Chinese medicine raw medicinal material compatibility mode; Therapeutic effect significantly, integrated morphology more closely, specific aim is stronger, cure rate is higher, it is credible to become to distinguish one from the other, and has no side effect, and takes the safe cancer anti-cancer prescription of controlling.
The gene mutation that the present invention's prescription is used for treatment and prevents to be caused by AFB1 (AFB1), DMBA, benzopyrene, urethanes (the urethane EC that fermented food and ethanol beverage exist), AOM (AOM), free radical, cytosol APC Protein kinase C (PKC) and after birth LCK (TPK); Cyclophosphamide brings out PCE (bone marrow polychromatophilia erythrocyte) micronucleus to chromosomal destruction, leukocyte DNA chain rupture or gene mutation that N-N-nitrosodimethylamine, myristoyl Buddhist skin alcohol ethyl ester (TPA), smoke from cigarette (CSC) condensation product cause.Be used for the tumor that retrovirus diffusion causes, and the above-mentioned factor causes the tumor that forms after the gene mutation, cancerometastasis or the like.
The example Cordyceps tumor recovering side oral liquid that the present invention also is specifically related to this prescription prepares production technology.
Technical background
Known cancer is undergone mutation by gene, and diffusion causes and forms with retrovirus.The factor of modificator gene sudden change and retrovirus diffusion is diversified.
The key of treatment cancer be prevent, control, suppressor undergos mutation, and prevents, controls, eliminates retroviral diffusion.
The rapidity of the complexity of cancer, intractable and development and change has brought difficulty for this disease of treatment, although the struggle of the human tanacity of sparing no effort carrying out with it constantly,, fail to subdue this serious illness always.Sum up experience and lessons in the past, the doctor trained in Western medicine medication has got into the mistaken ideas that single active ingredient is cured the disease, and can't resist obstinate and cancer complicated and changeable.Middle medical drugs has followed traditional raw material medical material compatibility mode, and the targeting of medication is not strong, and toxic and side effects is bigger, has weakened therapeutic effect.In order to defeat the No.1 disease cancer that threatens human health, must change mode of thinking, become the Synergistic treatment of the treatment of doctor trained in Western medicine single component into numerous effective ingredient, improve the sick assurance coefficient of winning victory of gram.The raw medicinal material compatibility of medical drugs is the compatibility of effective ingredient in the change, has both greatly improved the effectiveness and the cure rate of target function, can reduce cost again, improves the utilization rate of raw medicinal material, eliminates the toxic and side effects that raw medicinal material had.Along with the development of membrane technology, molecules research, high-performance production and inspection machine, our extraction separation from relevant edible fungus or food plant or the plant that has no side effect has gone out the various effective ingredient of treatment tumor, to satisfy the needs of the present invention's prescription.
Although carcinogenic factor is a lot; As: N-N-nitrosodimethylamine, AFB1 (AFB1), DMBA, benzopyrene, urethanes, AOM (AOM), cytosol APC Protein kinase C (PKC), the after birth LCK factors such as (TPK) can both cause gene mutation; The PCE that cyclophosphamide brings out (bone marrow polychromatophilia erythrocyte) micronucleus can play destruction to chromosome; Myristoyl Buddhist skin alcohol ethyl ester (TPA), smoke from cigarette (CSC) condensation product can cause leukocyte DNA chain rupture or gene mutation, and retroviral diffusion energy directly forms nasopharyngeal carcinoma, pulmonary carcinoma, breast carcinoma, black cancer etc.Yet science compatibility of the present invention and effective ingredient wherein can effectively be eliminated their carcinogenesis.
The determination of treatment based on pathogenesis obtained through differentiation of symptoms and signs of the traditional Chinese medical science, Chinese medicine monarch, minister, the compatibility theory of helping, making are for the invention provides guiding theory and theoretical basis.
Cordyceps is one of China's Chinese medicine three big treasured, in cancer preventing and treating, has irreplaceable effect.The cordycepin that it contains, cordycepic acid, Cordyceps polysaccharide, Cordyceps lactone, Cordyceps alkaloid, linoleic acid etc. all are effective ingredient of anticancer.Because natural cordyceps is rare, can't satisfy the growing market demand.Through the effort of decades, we have developed with natural Cordyceps gene and effect corresponding to Cordyceps mycelium (Hirsutella Sinensis Liu Yu et Zeng).Identify that through Institute of Micro-biology of the Chinese Academy of Sciences Cordyceps mycelium of our Development and Production can substitute natural cordyceps fully.
The destruction that the contained Quercetin (quercetin) of papaw leaf can resist the radical pair cell is directly suppressed the propagation of tumor cell.Quercetin can with Cancer-causing mutation factor N-N-nitrosodimethylamine, AFB1 (AFB1), DMBA, benzopyrene, cyclophosphamide, (TPA) interaction of etc.ing of myristoyl Buddhist skin alcohol ethyl ester, realize suppressing the purpose that cancer is suddenlyd change.Quercetin reaches anticancer purpose through the activity that suppresses cytosol APC Protein kinase C (PKC) and after birth LCK (TPK).
Oleum Bulbus Allii can play the effect of anticancer propagation through blocking the synthetic of cancerous cell DNA and duplicating.Strengthen the antitumor action of interior neutrophilic granulocyte of tumor kitchen range and macrophage in addition.
DOPA contained in the horse Herba Gynurae bicoloris (Herba Portulacae) is bright, DOPA, saponin, n-3 fatty acid component, and chemosynthesis that can block N-N-nitrosodimethylamine suppresses the cancer sudden change.
Components such as the contained oleanolic acid of Herba Ecliptae, 1,8,9-trihydroxy-3-methoxy-benzo[4,5, Fructus seu radix camptothecae acuminatae (Fructus Camptothecae Acuminatae) glycosides A, Fructus seu radix camptothecae acuminatae (Fructus Camptothecae Acuminatae) glycosides B, Fructus seu radix camptothecae acuminatae (Fructus Camptothecae Acuminatae) glycosides C, Fructus seu radix camptothecae acuminatae (Fructus Camptothecae Acuminatae) glycosides D, bosom moral lactone, tannin; Cyclophosphamide is brought out bone marrow polychromatophilia erythrocyte (PCE) micronucleus has the obvious suppression effect, and chromosome damage and hepatic injury are had protective effect and stronger active anticancer.
The contained ursolic acid of Folium Ilicis can suppress Buddhist ripple ester (TPA) is induced skin carcinoma to DMBA (DM-BA), benzopyrene effect.Ursolic acid derivant pair cell P388, L1210, human lung tumor's cell A-549 etc. have cytotoxicity, thus the growth of anticancer and propagation.
Contained tannin, the gallic acid of Galla Chinensis can suppress retroviral and transcribe and the duplicating of cancerous cell.
The chromone (5 that Rhizoma Smilacis Glabrae is contained; 7-dihydroxy-chromone-3-0-a-L-rhamnose) the direct kill cancer cell of ability; Can suppress the increment of HeLa cell, Ehrlich ascites cells; Hepatocarcinoma, ehrlich ascites carcinoma etc. can treated and prevent to the mutagenic action that can suppress cancerigenic factor AFB1 (AFB1).
Fructus Ligustri Lucidi contains abundant Quercetin, ursolic acid, oleanolic acid, Fructus Ligustri Lucidi polysaccharide etc. controls the cancer effective ingredient.Wherein edible fungus such as Fructus Ligustri Lucidi polysaccharide and Cordyceps polysaccharide can promote lymphocyte transformation, increases splenocyte to sheep red blood cell plaque forming cells number, the immunosuppressive action of antagonism meticortelone.Can greatly improve immunity, suppress the HL DNA chain rupture that short cancer thing myristoyl Buddhist skin alcohol ethyl ester (TPA) and smoke from cigarette (CSC) condensation product cause.
Ganoderma glucosan (gIu-can) (LB-NB) with the lactone composition; Can significantly promote the differentiation and proliferation of T cell, adenocarcinoma of lung (LA-795), hepatocarcinoma (7721), gastric cancer (7901), the esophageal carcinoma (Eca109), cervical cancer U14, leukemia, rectal cancer etc. are had prevention and therapeutical effect.
Quercetin, cordarine, cupreol, hyperin, chlorogenic acid component that Herba Houttuyniae is contained; Can significantly improve the ratio of periphery blood T lymphocyte; Can significantly reduce the leukocytoplania index; Improve the specificity Flos Rosae Rugosae and form cell number and neutrophil cell phagocytic rate, suppress the propagation of tumor cell.
The unsaturated fatty acid that Fish contain (oleic acid, linoleic acid, eicosatrienoic acid, arachidonic acid etc.), ability anticancer DNA's is synthetic, has antitumor action.
Herb Gynostemmae Pentaphylli total glycosides can suppress the transfer of pulmonary carcinoma TIS Lewis growth and cancerous cell, can suppress growth and DNA, RNA and proteinic the synthesizing of HCC.
Summary of the invention
Provide that a kind of treatment and prevention cancer are brought out, the prescription and the example Cordyceps tumor recovering side oral liquid of oncogene mutation, growth of cancer cells propagation, cancerometastasis prepare production technology.
The present invention relates to that a kind of cure rate is high, preventive effect is good, take safe treatment, the prevention cancer is brought out, the prescription of oncogene mutation, growth of cancer cells propagation, cancerometastasis.The preparation production technology that relates to example Cordyceps tumor recovering side oral liquid.The edible composition and the edible property that relate to this formulation product, promptly extraction separation comes out from edible fungus and food plant controls the cancer effective ingredient, can better treat and prophylaxis of cancer.The present inventor finds; Extraction separation comes out in food plants such as edible fungus such as Cordyceps and papaw leaf, Fructus Ligustri Lucidi, horse Herba Gynurae bicoloris controls the cancer composition, can in the destruction of cancerigenic factor N-N-nitrosodimethylamine, AFB1 (AFB1), DMBA, benzopyrene, cyclophosphamide, myristoyl Buddhist skin alcohol ethyl ester (TPA) etc.; Suppress oncogene mutation such as pulmonary carcinoma, hepatocarcinoma, gastric cancer, breast carcinoma, intestinal cancer, uterus carcinoma, esophageal carcinoma, osteocarcinoma, bladder cancer, lymphatic cancer, nasopharyngeal carcinoma, cancer of pancreas, skin carcinoma, the brain cancer, leukemia, growth of cancer cells propagation, tumor formation and cancer cell metastasis etc.; Can also the direct killing cancerous cell, improve body immunity, recover the health level of human body.
The representative example of above-mentioned cancer is pulmonary carcinoma and rectal cancer.
For high efficiency, novelty and the safety of checking this prescription, with this formulation Cordyceps tumor recovering side oral liquid, and carried out clinical experiment, explored Cordyceps tumor recovering side oral liquid process for producing.This preparation technology mainly is through medicine-food two-purpose fungus or the step of plant and the steps of membrance separation gained extract such as extraction Cordyceps, papaw leaf, horse Herba Gynurae bicoloris, Fructus Ligustri Lucidi, produces the step of products such as Cordyceps tumor recovering side oral liquid etc. according to prescription.
The present invention relates to treat and to prevent that cancer is brought out, oncogene mutation, growth of cancer cells propagation, tumor forms and the compositions of cancer cell metastasis effective ingredient, said composition contains above-mentioned effective ingredient and pharmaceutically acceptable carrier.
Said composition can be chosen dosage forms such as powder, liquid (containing decoction), tablet, capsule, unguentum, granule, piece agent, pill, medicated wine, sugar-coat agent, gel, syrup, serosity, suspensoid, medicinal tea.
One, prescription
(1) compatibility unit
The first compatibility unit stops or prevents the compatibility of gene mutation
Known N-N-nitrosodimethylamine, AFB1 (AFB1), DMBA, benzopyrene, cyclophosphamide, myristoyl Buddhist skin alcohol ethyl ester (TPA) etc. are the factors of oncogene sudden change.Quercetin (quercetin) is through with the interaction of these cancerigenic factors with suppress the activity of cytosol APC Protein kinase C (PKC) and after birth LCK (TPK); The propagation that can stop gene mutation and cancerous cell, the destruction that can stop the radical pair cell.
(consumption sees that compatibility is synthetic)
With components such as oleanolic acid, 1,8,9-trihydroxy-3-methoxy-benzo[4,5, demethylwedelolactone, Fructus seu radix camptothecae acuminatae (Fructus Camptothecae Acuminatae) glycosides A, Fructus seu radix camptothecae acuminatae (Fructus Camptothecae Acuminatae) glycosides B, Fructus seu radix camptothecae acuminatae (Fructus Camptothecae Acuminatae) glycosides C, Fructus seu radix camptothecae acuminatae (Fructus Camptothecae Acuminatae) glycosides D, bosom moral lactone, tannins; Suppress cyclophosphamide and bring out bone marrow polychromatophilia erythrocyte (PCK) micronucleus; The protection chromosome is avoided damage and hepatic injury, stops gene to be undergone mutation.
(consumption sees that compatibility is synthetic)
, DOPA bright with DOPA, saponin, n-3 fatty acid component, the chemosynthesis of block N-N-nitrosodimethylamine prevents its modificator gene sudden change.
(consumption sees that compatibility is synthetic)
Activity with Oleum Bulbus Allii reduction cancerous cell archaeal dna polymerase a blocks the synthetic of cancerous cell DNA and duplicates.Blocking-up aminophenazone and sodium nitrite form N-nitrosodimethylamine in vivo.
(consumption sees that compatibility is synthetic)
With synthesizing of unsaturated fatty acid (oleic acid, linoleic acid, eicosatrienoic acid, arachidonic acid) anticancer DNA, the retardance gene mutation.
(consumption sees that compatibility is synthetic)
The neoplastic compatibility of growth of cancer cells, propagation and cancerous protuberance is controlled in the second compatibility unit
Form cell toxicant, the growth of anticancer or other tumor cell, propagation with ursolic acid and derivant pair cell P388 thereof, L1210, lung tumor cell A-549 etc.Interior stream and the TPA (Buddhist ripple ester) of release and extracellular Ca2+ that suppresses Ca2+ in the cell lures cancer effect (skin carcinoma) to DMBA (DM-BA).
(consumption sees that compatibility is synthetic)
With Quercetin, cordarine, cupreol, hyperin, chlorogenic acid component, improve the ratio of periphery blood T lymphocyte, reduce the leukocytoplania index, improve the specificity Flos Rosae Rugosae and form cell number and neutrophil cell phagocytic rate, the propagation of anticancer.
(consumption sees that compatibility is synthetic)
The compatibility of cancer cell metastasis is controlled in the 3rd compatibility unit
With ursolic acid, TANSHINONES first, TANSHINONES second component, suppress the invasion by tumor cells adhesive capacity, it can't be hidden oneself and spread.
(consumption sees that compatibility is synthetic)
With Cordyceps polysaccharides, Radix Rubiae lactone, 6-hydroxyl-2H-naphthalene a pair of horses going side by side [1-2-6] pyran-2-one-components such as 5-carboxylate methyl ester; Prevent that oncocyte from shifting or diffusion, suppress the propagation of cancerous cell such as leukemia, ehrlich ascites carcinoma, colorectal cancer, pulmonary carcinoma, hepatocarcinoma, gastric cancer, breast carcinoma, uterus carcinoma, esophageal carcinoma, osteocarcinoma, bladder cancer, lymphatic cancer, nasopharyngeal carcinoma, cancer of pancreas, skin carcinoma, the brain cancer.
(consumption sees that compatibility is synthetic)
The 4th compatibility unit kills and wounds the compatibility of cancerous cell
Propagation with chromone direct killing cancerous cell and anticancer.And the Cancer-causing mutation effect of inhibition AFB1 (AFB1).
(consumption sees that compatibility is synthetic)
With components such as fumaric acid, monomethyl fumarate, pyranglucoside, ursolic acid, the acid of the blue flower principal columns of a hall, Rhizoma Kaempferiae glycosides, killing hepatoma (7721), gastric cancer (7901), esophageal carcinoma (Eca109), nasopharyngeal carcinoma cell etc.
(consumption sees that compatibility is synthetic)
With α-Gu Yunxi, β-gurjunene, 3; 7; Components such as 11-trimethyl 12 tetraenes, Geniposidic acid, asperuloside, isopinocamphone are killed and wounded pulmonary carcinoma, ovarian cancer, uterus carcinoma, osteocarcinoma, rectal cancer, hepatocarcinoma, bladder cancer, anus cancer, esophageal cancer cell etc.
(consumption sees that compatibility is synthetic)
With the growth of Herb Gynostemmae Pentaphylli total glycosides inhibition pulmonary carcinoma original position tumor Lewis, anticancer DNA, RNA and proteinic synthetic.
(consumption sees that compatibility is synthetic)
With components such as cordycepin, cordycepic acid, Cordyceps lactone, Cordyceps alkaloid, linoleic acid, the growth of anticancer, propagation and conversion.
(consumption sees that compatibility is synthetic)
The compatibility of retrovirus diffusion is controlled in the 5th compatibility unit
Nearly 8% is to be made up of the virus of once attacking ancestors in the known human genome, and virus has disappeared, but the hereditary material of virus has been stayed on host's the DNA.These viruses that can on host DNA, stay hereditary material are not general virus, are retrovirus.Retrovirus can not bred, and it can only be transcribed and duplicate after inserting gene.Yet the retrovirus that has made a variation can't transcribe and duplicate because of having lost the activity of transcribing and duplicating again, on the contrary, has but possessed the ability of resisting other virus infection identical carrier.
In endogenous retrovirus; Also have such a case: they are in case activated by certain factor; Just can recover original person's character, or new exogenous retrovirus intrusion people human body, to it condition of recovering originality is provided; Can spread in vivo very soon and come, form cancers such as pulmonary carcinoma, nasopharyngeal carcinoma, breast carcinoma, skin carcinoma.
Components such as ursolic acid, Quercetin, tannin, gallic acid, R-pulegone, S-pulegone, martairesinol, arctigenin; Can effectively block retrovirus and insert transcribing and duplicating behind the gene; Neutralization activates the effect of the endogenous retrovirus factor effectively, thereby reaches the purpose of controlling the retrovirus diffusion.
(consumption sees that compatibility is synthetic)
The 6th compatibility unit improves immunity and the ability of resisting cancer, realizes equilibrium between yin and yang, the level of getting well.
With components such as oleanolic acid, rhamnose, Cordyceps polysaccharides, general polysaccharide, lactone compounds, promote lymphocyte transformation, increase splenocyte to sheep red blood cell plaque forming cells number, the immunosuppressive action of antagonism meticortelone.Promoting leucocytes promotes the ability of leukocytes phagocytic free radical and antibacterial, suppressor mutation, the equilibrium between yin and yang of each side in the realization body, the health level of recovery human body.
(consumption sees that compatibility is synthetic)
(2) compatibility is synthetic
Quercetin,
Oleanolic acid, 1,8,9-trihydroxy-3-methoxy-benzo[4,5, demethylwedelolactone, Fructus seu radix camptothecae acuminatae (Fructus Camptothecae Acuminatae) glycosides A, B, C, D, bosom moral lactone, DOPA is bright, DOPA, saponin, n-3 fatty acid, Oleum Bulbus Allii, unsaturated fatty acid.
Consumption is: 1mg-6g/kg
Ursolic acid, cordarine, cupreol, hyperin, chlorogenic acid.
Consumption is: 1mg-2.4g/kg
Cordyceps polysaccharides, TANSHINONES first, second, Radix Rubiae lactone, 6-hydroxyl-2H-naphthalene a pair of horses going side by side [1-2-6] pyran-2-one-5-carboxylate methyl ester.
Consumption is: 1mg-3.6g/kg
Cordycepin, cordycepic acid, Cordyceps lactone, Cordyceps alkaloid, chromone, fumaric acid, monomethyl fumarate, pyranglucoside, ursolic acid, the acid of the blue flower principal columns of a hall, Rhizoma Kaempferiae glycosides; α-Gu Yunxi, β-gurjunene, 3; 7,11-trimethyl 12 tetraenes, Geniposidic acid, asperuloside, isopinocamphone; Herb Gynostemmae Pentaphylli total glycosides, linoleic acid.
Consumption is: 1mg-5.4g/kg
Tannin, gallic acid, R-pulegone, S-pulegone, martairesinol, arctigenin.
Consumption is: 1mg-2.4g/kg
Rhamnose, general polysaccharide.
Consumption is: 1mg-2.4g/kg
(annotate: mark " _ _ _ _ " line person is double amount)
(3) prescription characteristic
1, the compatibility level is good, and is scientific and reasonable for structure
According to the evolution of the cause of disease, pathology and cancer, divide six levels to carry out compatibility, solved the different problems that the cancer different phase occurs.The one, solved various cancerigenic factor modificator gene sudden change problems.The 2nd, solved retrovirally transcribe, duplicate, diffusion problem.The 3rd, after having solved tumor and forming, control its development problem.The 4th, solved the cancerometastasis problem that occurs in the tumor development.The 5th, after having solved cancerous cell and generating,, directly kill the cancerous cell problem not damaging under the normal cell situation.The 6th, solved problem a little less than the resistance against diseases that causes because of hypoimmunity.
In the compatibility structure aspects, contained the overall process of the cause of disease, pathology and cancer development, there are not perforated scrofula of neck and leak.Aspect the taboo of treatment and prevention composition, the factor of not cancelling each other and restricting, the combination of various compositions has formed the effect of 1+1＞2.
2, coordinative role is big, and therapeutic effect is remarkable, and is safe and reliable, rewritten the history of cancer as incurable disease
Prescription of the present invention is 100% to the effective percentage of tumor cell, is 99.9% to the kill rate of cancerous cell.Why effect is so good, is because this prescription has been given full play to the united and coordinating effect of each effective ingredient, has shown concentrated dominant component, the sick characteristic of winning victory of gram.The present invention is the commander with the Cordyceps polysaccharides, coordinates various effective ingredient, has given full play to maximum function separately, has constituted powerful overall strength.To the complexity of cancer, utilize corresponding effective ingredient, defeat in detail.To the intractable of tumor disease, the putting down of intersection of taking to classify killed strategy it subdued.To the rapidity of cancer development, with the way of removing burning wood away under the boiler, control the growing multiplication of cancerous cell, it can't be developed.
3, with strong points
The cause of disease, pathology and its development and change process according to cancer; What composition is effective to treatment and prophylaxis of cancer; Just what become is divided into groups in the side of going into, forms powerful killing and prevent cancer is brought out, oncogene mutation, growth of cancer cells propagation, cancerometastasis with joint efforts, cancer complicacy and obstinate how no matter; Can both it be killed, reach the purpose of healing.
4, become to distinguish one from the other, do not feel uncertain
Chinese medicine is because of being unable to explain clearly therapeutic component, and externally state medical institutions have caused some to feel uncertain with the part patient, has influenced the Chinese medicine outlet greatly and to the confidence level of Chinese medicine.Because prescription of the present invention has been taked the compatibility mode of effective ingredient, its therapeutic component is fully aware of, and action effect is also very significantly finally captured cancer with the mankind and will be made due contribution for Chinese medicine goes to the world.
5, have no side effect
The effective ingredient of the treatment cancer that the present invention selected for use all comes from edible fungus, food plant or non-toxic herb, has no toxic and side effects.In toxic and side effects test, also strong proof this point.Show fully that with this saying of " dietetic therapy is better than medication " has certain reason.Show simultaneously, from food, also can separate the good medicine that compatibility becomes the thorny disease of treatment, and take saferly than the medicine of chemosynthesis and the medicine that from the toxic side effect plant, separates compatibility, act on better.
6, save raw material, can make the bigger wider effect of raw medicinal material performance
The contained composition of raw medicinal material is a lot, and the treatment cancer only needs a part wherein, and all the other compositions can be used for treating other disease.So, take the effective ingredient prescription, than the traditional raw material compatibility mode, can save a large amount of material compositions, make the bigger wider effect of its performance.
Two, preparation technology
Prescription provided by the invention; With extraction separation treatment and prevention cancer bring out, oncogene mutation; Growth of cancer cells propagation, tumor form, the effective ingredient preparation technology of cancerometastasis is the basis; Under the situation that the needed various effective ingredient of prescription all possess,, form the medicament of treatment and prevention tumor according to the prescription requirement.Its example is Cordyceps tumor recovering side oral liquid and preparation technology thereof.
(1) extraction separation preparation technology
The active ingredient of treatment used in the present invention and prevention tumor; Be from medicine food dual purpose plants such as the mycelium of Cordyceps sporophore or artificial culture or horse Herba Gynurae bicoloris, Fructus Ligustri Lucidi, papaw leaf, Radix Ginseng, to extract; Molecular weight is respectively the plurality of active ingredients of 180 (glucosans), 302 (Quercetins), 456 (ursolic acids), 460 (Fructus Ligustri Lucidi oleanolic acid), 572 (arasaponins), 800 (ginsenosides), 10000 (Cordyceps polysaccharides) etc.; (the extraction and separation process flow chart is seen accompanying drawing) forms the medicament of treating and preventing tumor through compatibility.
For this reason, the present invention proposes to utilize technology such as extraction and membrance separation, and to Cordyceps sporophore, Cordyceps mycelium or food plant are effectively extracted and isolating unified preparation technology.
The step of this technology is, with the ethanol of 15-95% the cancer composition of controlling that Cordyceps or Cordyceps mycelium, food plant etc. contain extracted earlier, obtains small molecule component.Then ethanol extract being used molecular weight respectively is that 302,456,572,800,946,2000 film filters repeatedly, is arranged in order according to the molecular weight size, collects unsanctioned composition of per stage and carries out drying, is needed effective ingredient.The present inventor claims that this technology is alcohol extraction process.
In gathering in alcohol extraction step, used concentration of ethanol is 15-95%, and consumption is the 6-20 doubly [weight (kilogram)/volume (liter) compares] of extraction feed amount, and the extraction time is 1-2 hour at every turn, continuous extraction 1-3 time (seeing embodiment 1).
Residue after the said method alcohol extraction, the oven dry back is heated to 50-100 ℃ with extraction feed 6-20 water (weight ratio) doubly and extracts, and extracts 20-30 minute at every turn, carries out 2-3 time continuously.Through the orthogonal experiment design, the optimum process condition of confirming is: the amount of institute's water is 10 times of extraction feed amount (weight), extracts 30 minutes at every turn, carries out continuously 3 times (seeing embodiment 2).
Three extracts are merged, before membrane filtration, use a certain amount of clarifier in case of necessity, like 101 fruit juice clarifiers, ZTC1+1 natural clarifying agent etc.Water extract after the clarification is by descending the putting in order of molecular weight; Membrane filtration repeatedly through part, is carried out concentration on per stage film successively; Obtain needed various effective ingredient, directly be made into liquid preparations such as Cordyceps tumor recovering side oral liquid then.Or process Powdered composition through vacuum drying, spray drying, cryodesiccated method, be used for other various preparations (seeing embodiment 2).The effective ingredient of various Cordyceps, the food plant that makes like this, the treatment tumor of the plant that has no side effect; Can use the known HPLC of insider methods such as (HPLies) to obtain, can use NMR method methods such as (nuclear magnetic resonance spectroscopy methods) to identify the gained constituent structure.
The specific embodiment
Plant such as embodiment 1 alcohol extraction Fructus Ligustri Lucidi, papaw leaf, Cordyceps or fungus are removed the process optimization of micromolecule composition
1, extract the orthogonal experiment optimization of food plant such as Fructus Ligustri Lucidi, papaw leaf, Cordyceps or fungus technology:
(1) Orthogonal Experiment and Design:
A: concentration of alcohol
A
1?0％；A
2?15％；A
3?30％；A
4?45％；A
5?60％；A
6?75％；A
7?85％；A
8?95％；
B: sample (kilogram)/quantity of solvent (liter)
C: extraction time (h)
C
1?1.5；C
2?3.0
D: extraction time
D
1?2；D
2?3
According to above factor and design levels L
16(8 * 2
8) orthogonal table sees table 6.
Table 6 L
16(8 * 2
8) the gauge outfit design
(2) judge index and mensuration result's (is example with the Fructus Ligustri Lucidi):
Evaluation of this orthogonal experiment and judge index are content and Quercetin, the ursolic acid of Quercetin in the Fructus Ligustri Lucidi extractum, the total amount of oleanolic acid.Its content all adopts the HPLC-ELSD method to measure.Experimental result is seen table 7.
(3) variance analysis:
Measure the result according to above index property component content, carry out variance analysis with the total amount of Quercetin and ursolic acid, oleanolic acid, Quercetin respectively, the result sees table 8 and table 9.
(4) experimental result: the The results of analysis of variance of table 8 and table 9 shows, no matter is to be separately evaluation index with the Quercetin, and still the total amount with Quercetin, ursolic acid, oleanolic acid is an evaluation index; Orthogonal experiment results all shows A6, B2, C1/C2; D2 is an optimum process condition; Promptly use 75% ethanol, consumption is to be 1.5-3 hour 1: 8 extraction time, extracts continuously 2 times.
Table 7 is respectively organized experimental index property composition measurement result
Table 8. is the variance analysis of index with the Quercetin
Table 9 is the variance analysis of index with Quercetin, ursolic acid and oleanolic acid summation
The preparation technology and the optimization thereof of water solublity Fructus Ligustri Lucidi polysaccharide (LLPS) after the embodiment 2. Fructus Ligustri Lucidi alcohol extraction remove impurity
1, the foundation of index property component content method
Classical Fructus Ligustri Lucidi polysaccharide content assaying method is to measure total sugar content earlier, deducts reducing sugar again, because Fructus Ligustri Lucidi is through 75% ethanol extraction, micromolecular reducing sugar has extracted fully, so directly with phenol sulfuric acid method mensuration Fructus Ligustri Lucidi polysaccharide content.
(1) standard curve and the range of linearity
Accurately take by weighing Fructus Ligustri Lucidi rhamnose 20.0mg in the 500ml volumetric flask, add water to scale, mixing.
Draw standard solution 0.4ml respectively, 0.6ml, 0.8ml, 1.0ml, 1.2ml; 1.4ml, 1.6ml and 1.8ml, each is mended to 2.0 ml with water, adds 6% phenol 1.0ml and concentrated sulphuric acid 5.0ml then; Left standstill 10 minutes, and shook up, room temperature is placed 20 minutes later in 490nm photometry density, is blank with 2.0ml water by same color operation; With the polysaccharide micrograms is abscissa, is vertical coordinate with the optical density, and the drawing standard curve also calculates regression equation, and the result sees table 10.
Table 10 standard curve test data
The above results shows that the method that phenol sulfuric acid method is measured polyoses content concerns good in 16-72 μ g scope internal linear: Y=0.0158
X-0.00351R
2=0.9996
(2) application of sample recovery test
Accurately take by weighing the Fructus Ligustri Lucidi polysaccharide sample 250mg of a collection of known content, put in the 50ml volumetric flask, be dissolved in water, be diluted to scale, precision is measured 3 parts of 0.8ml respectively; 3 parts of 1.0ml, 3 parts of 1.2ml; Respectively add the 0.5ml standard solution, thin up is to 100ml, and is subsequent use.
Precision is measured above-mentioned solution 1.0ml, adds water 1.0ml, adds 6% phenol 1.0ml and concentrated sulphuric acid 5.0ml then, leaves standstill 10 minutes, shakes up, and room temperature is placed 20 minutes later in 490nm photometry density, calculates polyoses content according to standard curve, and the result sees table 11.
Annotate: the dilution of sample coefficient of three kinds of concentration is respectively 0.8,1.0 and 1.2.
Table 11 application of sample recovery test determination data
(3) precision test
Precision takes by weighing 6 parts in a collection of Fructus Ligustri Lucidi polysaccharide sample, and every part of 250mg puts in the 50ml volumetric flask, is dissolved in water, and is diluted to scale, and precision is measured 1.0ml respectively, and thin up is to 100ml, and is subsequent use.
Precision is measured above-mentioned solution 1.0ml, adds water 1.0ml, adds 6% phenol 1.0ml and concentrated sulphuric acid 5.0ml then, leaves standstill 10 minutes, shakes up, and room temperature is placed 20 minutes later in 490nm photometry density, calculates polyoses content according to standard curve, and the result sees table 12.
Table 12 replica test data (n=6)
The result shows: phenol sulfuric acid method is measured the content of these article polysaccharide, and method precision is better, RSD%＜1%.
(4) reaction back sample solution stability
The sample solution of getting above-mentioned reaction is a, and respectively 0, measure absorbance in the 30min, 60min, 90min, 120min, the result sees table 13.
Table 13 stability test data
| Time | 0 | 30min | 60min | 90min | 120min | RSD |
| A | 0.638 | 0.637 | 0.638 | 0.639 | 0.637 | 0.12％ |
The result shows: reaction back sample solution is stable in 2 hours.
2, orthogonal test
(1) Orthogonal Experiment and Design
A, factor and level
A factor (extraction time) 1 level: 1 time
2 levels: 2 times
3 levels: 3 times
B factor (extraction time) 1 level: 1 hour
2 levels: 2 hours
3 levels: 3 hours
C factor (consumption of water) 1 level: 1: 10
2 levels: 1: 8
3 levels: 1: 6
B, gauge outfit design
Table 14 L9 (34) orthogonal table
| № | A | B | C | Err |
| 1 | 1 | 1 | 1 | 1 |
| 2 | 1 | 2 | 2 | 2 |
| 3 | 1 | 3 | 3 | 3 |
| 4 | 2 | 1 | 2 | 3 |
| 5 | 2 | 2 | 3 | 1 |
| 6 | 2 | 3 | 1 | 2 |
| 7 | 3 | 1 | 3 | 2 |
| 8 | 3 | 2 | 1 | 3 |
| 9 | 3 | 3 | 2 | 1 |
(2) test method
The preparation of a need testing solution
Accurately take by weighing Fructus Ligustri Lucidi residue 150g, the water by 8-12 times of volume of gauge outfit designs C row adding soaked 30 minutes; Put respectively in the electric jacket, be heated to boiling after, keep little boiling 1-3 hour by B row respectively; Press A row respectively and extract 1-3 time, merge extractive liquid,, 60 ℃ be evaporated to proper volume after, add water and be settled to 100ml, precision is measured 1.0ml, puts in the 250ml volumetric flask, adds water to scale, mixing.
The B sample size is measured
Draw need testing solution 1.0ml, by the above-mentioned steps operation, record OD value, calculate polyoses content with standard curve, the result sees table 15.
Table 15 assay result
With the extraction ratio is to investigate index, and result of the test is carried out variance analysis
The variance analysis of table 16 orthogonal experiments
| Soruces of variation | df | SSj | MSj | F | Sig· |
| A | 2 | 6.1908 | 3.0954 | 359.47 | ** |
| B | 2 | 0.1882 | 0.0941 | 10.92 | ? |
| C | 2 | 0.8401 | 0.4200 | 48.78 | * |
| Err· | 2 | 0.0172 | 0.0086 | ? | ? |
F
u＝0.01(2，2)＝99；F
u＝0.05(2，2)＝19；F
u＝0.10(2，2)＝9；
ANOVA showed significant A factor is the most remarkable, secondly is the C factor, and the B factor is not remarkable in the a=0.05 level, and is remarkable in the a=0.01 level.
The b multiple comparisons need carry out multiple comparisons because each factor has three levels.
The multiple comparisons of table 17A factor
| ? | Adjacent | Between |
| K 3(6.54) | ? | ? |
| K 2(6.35) | 0.19 | ? |
| K 1(4.69) | 1.66 | 1.85 |
| Q 0.05S X | 0.3255 | 0.4995 |
A factor (extraction time) multiple comparisons shows A
3Level and A
2Level does not have significant difference, considers that from economic angle extraction time is chosen A
2Level is promptly extracted 2 times.
Table 18 B factor multiple comparisons
| ? | Adjacent | Between |
| K 3(5.97) | ? | ? |
| K 2(5.96) | 0.01 | ? |
| K 1(5.65) | 0.31 | 0.32 |
| Q 0.05S X | 0.3255 | 0.4995 |
The result of B factor multiple comparisons is consistent with variance analysis, and is not remarkable in the a=0.05 level, B3 level and B2 zero difference, but they differ greatly with B1, but not remarkable in 0.05 level, so the B factor is chosen the B2 level, promptly extracted 2 hours.
The multiple comparisons of table 19 C factor
| ? | Adjacent | Between |
| K 1(6.26) | ? | ? |
| K 25.80 | 0.46 | ? |
| K 35.52 | 0.28 | 0.74 |
| Q 0.05S X | 0.3255 | 0.4995 |
C factor multiple comparisons shows that the C1 level is significantly higher than other two levels, because the C factor is in top level, needs supplementary test, to confirm the optimum solvent ratio.
3, supplementary test
Accurately take by weighing 6 parts of 150g of Fructus Ligustri Lucidi residue, 3 parts add water 1500ml; 3 parts add water 1800ml, soak 30 minutes; Put respectively in the electric jacket, be heated to boiling after, keep little and boiled 2 hours; Extract 2 times, merge extractive liquid,, 60 ℃ be evaporated to proper volume after, add water and be settled to 1000ml, precision is measured 1.0ml, puts in the 250ml volumetric flask, adds water to scale, mixing.
Table 20 chases after test data
The result shows, the extraction ratio that the quantity of solvent increase can not significantly increase polysaccharide with extract total amount, so the C factor is decided to be the C1 level, promptly the ratio in 1: 10 (V/W) adds water.
4, conclusion
According to above orthogonal test and supplementary test, show that the optimised process that residue after the Fructus Ligustri Lucidi alcohol extraction extracts polysaccharide is:
The Fructus Ligustri Lucidi residue was soaked 30 minutes in 1: 10 ratio (weight ratio), was heated to the boiling back and kept little boiling 2 hours, extracted 2 times, and merge extractive liquid, filters, and 60 ℃ are evaporated to dried.
5, technology stability checking
Accurately take by weighing 5 parts of Fructus Ligustri Lucidi residues, every part of 60g adds water 600ml respectively, soaks 30 minutes, after electric jacket is heated to boiling, keeps little and boils 2 hours, extract 2 times, and merge extractive liquid,, 60 ℃ are evaporated to dried.
Accurately take by weighing above-mentioned polysaccharide sample, every part of 250mg puts in the 50ml volumetric flask, is dissolved in water, and is diluted to scale, and precision is measured 1.0ml respectively, and thin up is to 100ml, and is subsequent use.
Accurately measure above-mentioned solution 1.0ml, add water 1.0ml, add 6% phenol 1.0ml and concentrated sulphuric acid 5.0ml then, left standstill 10 minutes, shake up, room temperature is placed after 20 minutes in 490nm photometry density, according to standard curve calculating polyoses content
Table 21 technology stability test data
The result shows that this extraction process is stable, RSD＜1%
The water of embodiment 3, Fructus Ligustri Lucidi water soluble polysaccharide is put forward membrane filtration technology
Fructus Ligustri Lucidi, coarse powder 12000kg is heated to 20-105 ℃ with the water of 10 times of amounts (weight ratio) and extracts, and extracts 30 minutes at every turn, extracts merge extractive liquid, continuously 3 times.In extracting solution, add 5% ZTC1+1 natural clarifying agent, leave standstill after fully stirring and put coldly, filter with centrifugal filtration process and to obtain clarifying extracting solution.Extracting solution is pumped in the membrane filter, and using molecular cut off is that 4,000 filter membrane filters; After several is carried out in circulation, collect and do not pass through part on the film, be concentrated to thick extractum (proportion-1.10) with Rotary Evaporators; The reuse vacuum drying oven is dry, pulverizes, and promptly gets Fructus Ligustri Lucidi water soluble polysaccharide 68kg.
In like manner, with reference to said method, other active ingredient of the needed treatment tumor that obtains filling a prescription.
(2) example Cordyceps tumor recovering square opening clothes liquid preparing process
Require and the active ingredient of the ratio combined treatment cancer prescription of compatibility according to above-mentioned prescription, add water for injection in proportion and be diluted to 20 tons, add 16 kilograms of saccharin sodium; Make it dissolving, left standstill 24 hours, filter; Add water adjustment total amount to 20 ton; Press the dose fill, sterilization promptly gets Cordyceps tumor recovering side oral liquid (this preparation technology's flow chart is seen accompanying drawing 2)
Three, test situation
The present invention has carried out zoopery to above-mentioned Cordyceps tumor recovering side oral liquid; Show this medicine no side effects; The tumor that gene mutation, retrovirus are caused and the growing multiplication of cancerous cell, the diffusion transfer of cancerous cell has significant inhibitory effect; Have tangible immunoloregulation function simultaneously, concrete result of the test is following:
Test Example 1: acute toxicity testing
Acute toxicity test in mice is the result show, with Cmax, maximum administration volume is irritated stomach and given mice, and its maximum dosage-feeding is 75.0g/kg, is about 30 times of clinical RD.
Test Example 2: Cordyceps tumor recovering side oral liquid is to the inhibitory action of mice S180 sarcoma.
(1) test objective
Test Cordyceps tumor recovering side oral liquid is to the inhibitory action of mice S180 sarcoma growth.
(2) receive trial product
Title: Cordyceps tumor recovering side oral liquid (C), the inventor prepares voluntarily.
Preparation: use the distilled water wiring solution-forming
(3) control sample
Polysaccharide-peptide capsule, Shanghai Xinkang Pharmaceutical Factory produces, batch number: 060801 specification: every 0.34g.
(4) animal
Kunming mouse, male, body weight: 18-22g, is provided by the big medical college Experimental Animal Center of green grass or young crops by 10 every group.
(5) transplanted tumor: mice S180 sarcoma, blue or green big medical college Cytology Lab provides.
(6) test method
Get well-grown mice S180 sarcoma ascites, diluted with 1: 4, every the right axil subcutaneous vaccination of mice 0.2ml, random packet with normal saline.Cordyceps tumor recovering side oral liquid (C) establishes 0.5,0.25, three dose groups of 0.125g/kg, polysaccharide-peptide matched group 0.5g/kg, and administration is played next day in the inoculation back, and the administration volume is the 0.5ml/20g body weight, continuous irrigation stomach 7 days.Inoculate back 10 days and take off neck execution animal, weigh, dissect and get the tumor piece, claim that tumor is heavy.According to following formula result of determination:
(7) result of the test
Cordyceps tumor recovering side oral liquid (C) establishes 0.5,0.25 at dosage, during 0.125g/kg, and the suppression ratio of mice S180 sarcoma is respectively 99.90,88.27,72.40, and is more different with matched group.Result of the test is seen table 1
(8) conclusion (of pressure testing)
Cordyceps tumor recovering side oral liquid (C) has the obvious suppression effect to the growth of mice S180 sarcoma.
Table 1 Cordyceps tumor recovering side oral liquid (C) is to the tumor-inhibiting action of mice S180 sarcoma
Test Example 3, Cordyceps tumor recovering side oral liquid are to Immune Effects
1, to the influence of tumor-bearing mice spleen lymphocyte proliferation
(1) test objective
Observe of the effect of Cordyceps tumor recovering side oral liquid to the tumor-bearing mice spleen lymphocyte proliferation.
(2) receive the reagent thing
Title and compound method are with test 2.
(3) control sample
With test 2.
(4) female C57BL/6 mice, body weight: 18-20g is provided by the big medical college Experimental Animal Center of green grass or young crops.
(5) other material
Culture medium: RPMI-1610, the Difco product includes 15%NBS, mercaptoethanol, Hepes etc.
Con A (ConA): Sigma product, 50ug/ml.
(6) test method
30 of C57BL/6 mices, every Mus axil subcutaneous vaccination Lewis lung cancer cell about 2 * 10
5Individual, be divided into 5 groups at random, 6 every group, Cordyceps tumor recovering side oral liquid (C) 0.125,0.25,0.5g/kg, Polysaccharide-peptide capsule 0.5g/kg, normal saline matched group, all po * 7.Administration finishes the back and puts to death animal, gets spleen under the aseptic condition, the counting splenocyte, and the adjustment cell concentration is 1 * 10
7Individual/ml, every hole adds cell suspension 100ul on 96 well culture plates, ConA 50ul and culture fluid, and each group is all established three multiple holes, 37 ℃, 5%CO
2Cultivated 18 hours under the condition, add
3H-TdR 0.5uci/ hole continues to cultivate 18 hours, with bull cell harvestor collecting cell, on liquid scintillation instrument, surveys the CPM value, and compares with matched group, and the result sees table 2.
(7) result of the test
Cordyceps tumor recovering side oral liquid has the effect of tangible promotion tumor-bearing mice spleen lymphocyte proliferation when 0.125g/kg dosage, along with the increase of dosage, promote the spleen lymphocyte proliferation effect to strengthen thereupon.
Table 2 Cordyceps tumor recovering side oral liquid (C) is to the influence of tumor-bearing mice spleen lymphocyte proliferation
2, Cordyceps tumor recovering side oral liquid is to the influence of tumor-bearing mice NK cytoactive
(1) test objective
Observe of the influence of Cordyceps tumor recovering side oral liquid to tumor-bearing mice NK cytoactive
(2) receive the reagent thing
With test 2.
(3) control sample
Polysaccharide-peptide capsule, the source, lot number, specification is the same.
(4) experimental animal
Strain, body weight, sex and originate the same.
(5) other material
Culture medium:
3H-TdR is the same.The YAC-1 cell is provided by blue or green large hospital Cytology Lab.
(6) experimental technique
The labelling of target cell: get the back 24 hours well-grown YAC-1 cells that go down to posterity, by 1 * 10
6Individual/ml cell suspension adds
3H-TdR 10uci, in 37 ℃, 5%CO
2Cultivated 2 hours in the incubator, vibrated once in per 30 minutes, the cell behind the labelling is resuspended in the culture fluid with culture fluid washing 3 times, and making cell concentration is 1 * 10
5Individual/ml.
The NK cytoactive is measured: 30 of C57BL/6 mices, every Mus axil subcutaneous vaccination L ewis lung carcinoma cell about 2 * 10
6Individual, be divided into 5 groups at random, 6 every group, i.e. Cordyceps tumor recovering side oral liquid 0.125,0.25,0.5g/kg, Polysaccharide-peptide capsule 0.5g/kg, normal saline matched group, all po * 7.The last administration finishes the back and puts to death animal, gets spleen under the aseptic condition, the preparation splenocyte, and the adjustment cell concentration is 1 * 10
6Individual/ml does the effector lymphocyte, and other gets and cultivates 24 hours YAC-1 cells, and the adjustment cell concentration is 1 * 10
4Individual/ml is as target cell, is that 1: 100 cell is added on the 96 porocyte culture plates with target cell and effector lymphocyte's ratio, adds
3Three multiple holes, 37 ℃, 5%CO are all established for every group in H-TdR 0.5uci/ hole
2Cultivate under the condition after 24 hours, collecting cell is surveyed the CPM value, calculates specificity and suppresses percentage rate (Pi) expression NK cytoactive.
(7) experimental result
Successive administration can obviously activate the NK cell activity after 7 days, and dosage and facilitation have certain dependency, activated the polysaccharide-peptide that the NK cell activity is superior to Isodose during 0.5g/kg.The result sees table 3.
Table 3, Cordyceps tumor recovering side oral liquid are to the active influence of tumor-bearing mice NK
Compare with the normal saline group:
*P＜0.01.
3, Cordyceps tumor recovering side oral liquid is to the influence of tumor-bearing mice IL-2 generation
(1) test objective: observe the influence that Cordyceps tumor recovering side oral liquid produces tumor-bearing mice IL-2
(2) receive the reagent thing: the same
(3) control sample
Polysaccharide-peptide capsule, the source, lot number, specification is the same.
(4) experimental animal
Strain, body weight, sex, it is the same to originate.
(5) other material
Culture medium is the same.
(6) test method
30 of C57BL/6 mices, every Mus axil subcutaneous vaccination Lewis lung oncocyte about 2 * 10
5Individual, random packet administration, i.e. Cordyceps tumor recovering side oral liquid 0.125,0.25,0.5g/kg, Polysaccharide-peptide capsule 0.5g/kg, normal saline matched group, all po * 7.The last administration finishes the back and puts to death animal, gets spleen under the aseptic condition, the preparation splenocyte suspension, and the adjustment cell concentration is 1 * 10
7Individual/ml, every hole adds 2ml cell and ConA 5ug/ml, 37 ℃, 5%CO on 24 orifice plates
2Cultivate under the condition and collected supernatant in 24 hours, with IL-2 dependent cell strain CTLL, with
3It is active that the H-TdR method of mixing is measured IL-2, and compare with matched group, and the result sees table 4.
(7) experimental result
Cordyceps tumor recovering side oral liquid successive administration can promote the generation of IL-2 after 7 days, its 0.5g/kg group effect is the most obvious.
Table 4 Cordyceps tumor recovering side oral liquid exerts an influence to tumor-bearing mice IL-2
4, to the influence of Turnover of Mouse Peritoneal Macrophages phagocytic function
(1) test objective
Test Cordyceps tumor recovering side oral liquid is to the influence of Turnover of Mouse Peritoneal Macrophages phagocytic function
(2) receive the reagent thing
The same.
(3) control sample
Polysaccharide-peptide capsule, the source, lot number, specification is the same.
(4) experimental animal
Kunming mouse, body weight: 20-22g, male, provide by the big medical college animal center of green grass or young crops.Every treated animal number: 8.
(5) test method
Cordyceps tumor recovering side oral liquid is established three dose groups (0.125,0.25,0.5g/kg), and other establishes normal saline group, polysaccharide-peptide group (0.5g/kg), continuous oral administration 6 days; The last administration finishes the every Mus lumbar injection 20% chicken erythrocyte suspension 1ml in back, and animal is put to death in the cervical vertebra dislocation after 30 minutes, and the abdominal cavity injects normal saline 2ml; After rotating mice; Sucking-off abdominal cavity washing liquid is dripped on microscope slide, after rinsing, fixing, dyeing, carries out the macrophage counting under the oily mirror.
According to following formula result of determination:
(6) result of the test
Normal saline group mice phagocytic percentage and phagocytic index are respectively 20.21% and 0.22; Cordyceps tumor recovering side oral liquid successive administration is after 6 days; High, medium and low dose groups all can obviously activate the phagocytic function of Turnover of Mouse Peritoneal Macrophages; Phagocytic percentage is respectively 63.78%, 49.31% and 43.76%; Phagocytic index is respectively 0.68,0.53,0.47, and the action intensity of high, normal, basic dose groups all is superior to Polysaccharide-peptide capsule.Result of the test is seen table 5.
(7) conclusion (of pressure testing)
The oral liquid continuous oral administration of Cordyceps tumor recovering side is after 6 days, and high, medium and low dose groups all can obviously activate the phagocytic function of Turnover of Mouse Peritoneal Macrophages.
Table 5, Cordyceps tumor recovering side oral liquid influence the phagocytic function of Turnover of Mouse Peritoneal Macrophages
The present invention also proposes the purposes of said Cordyceps tumor recovering side effective ingredient, and it can be used to prepare Cordyceps tumor recovering side oral liquid separately, can also be used to prepare other medicines, also can be used to prepare food.
The medicine and the functional health-care food that prepare by extract of the present invention separately; Or compound medicine and the functional health-care food formed by this extract and other Chinese and western drugs or food; All have antitumor, regulate body immunity, pharmacologically actives such as health invigorating and energy can be used for: all kinds of tumors are treated and prevented in (1); Particularly the gene mutation of cell, retrovirus develop into the growth of tumor, propagation, diffusion.(2) strengthen that tumor is put, the curative effect of chemotherapy; (3) reduce that tumor is put, the toxicity, side effect of chemotherapy; (4) life quality of raising tumour patient, life-saving; (5) prevent and treat other Ia diseases, like rheumatism, rheumatoid, lupus erythematosus, AIDS, all kinds of hepatitis, diabetes, asthma, chronic bronchitis etc.; (6) immunity of raising human body is improved sub-health state, prevents and cures diseases.
Other combination when compatibility or this extract of this extract; When being used for above-mentioned medical treatment with the health care purpose; The method and the technology that can adopt this professional to know; Directly add necessary adjuvant, except that processing oral liquid, also can be made into several formulations finished products such as capsule, tablet, injection, granule, syrup, unguentum, medicated wine, beverage, fruit juice, instant tea, confection.When extract of the present invention was made into tablet, the excipient that it contains had: diluent, like starch, dextrin, lactose etc.; Wetting agent or binding agent, as: water, ethanol, starch slurry, dextrin, gelatine size, low-substituted hydroxypropyl cellulose, polyvinylpyrrolidone, Polyethylene Glycol etc.; Disintegrating agent, as: dry starch, gas-producing disintegrant, surfactant etc.; Lubricant is like Pulvis Talci, magnesium stearate, liquid paraffin, gather ethanol 6000 or 4000 etc.When the prescription of extract of the present invention was made into capsule with other combination, the excipient that it contains had: diluent, as: starch, dextrin, lactose, magnesium oxide, magnesium carbonate etc.; Wetting agent or binding agent, as: water, ethanol, starch slurry, dextrin slurry, gelatine size, low-substituted hydroxypropyl cellulose, polyvinylpyrrolidone, Polyethylene Glycol etc.; Disintegrating agent, as: dry starch, gas-producing disintegrant, surfactant etc.; And select gelatin hard softgel shell or soft capsule shell for use.When pharmaceutical composition of the present invention was made into injection, the excipient that it contains had: solubilizing agent, as: tween 80, glycerol etc.; Suspensoid, as; Hydroxy methocel, polyvinylpyrrolidone, methylcellulose etc.; Antioxidant, as: sodium sulfite, sodium pyrosulfite, sodium thiosulfate etc.; Osmotic pressure regulator is like sodium chloride or glucose etc.; The additives that ease the pain, as: benzyl alcohol, procaine hydrochloride etc.When pharmaceutical composition of the present invention was made into beverage, the excipient that it contains had: aqueous sucrose solution, correctives; Suspending agent is like hydroxy methocel, polyvinylpyrrolidone, methylcellulose etc.; Antiseptic is like Ethyl Hydroxybenzoate or Buddhist nun uncle tortoise beetle fat, propylene glycol, benzoic acid, sorbitol etc.
Fig. 1 is treatment and prevention tumor extracts active ingredients separation process scheme figure
Fig. 2 is a Cordyceps tumor recovering square opening clothes liquid preparing process flow chart.
Claims (11)
1. treat and prevention tumor prescription for one kind, it is characterized in that the compatibility of or magistery effective with multiple treatment tumor.It is made up of following method: contain in edible fungus such as Cordyceps that alcohol extraction, extraction, membrance separation are gone out or Cordyceps mycelium, Fructus Ligustri Lucidi, papaw and the medicine food dual purpose plant; Effective ingredient such as the cordycepin of treatment and prevention tumor, cordycepic acid, Cordyceps polysaccharide, Cordyceps lactone, Quercetin, ursolic acid, oleanolic acid, chromone, TANSHINONES; The cause of disease, pathology and its evolution according to cancer; Choose relevant composition targetedly, crack the complexity of cancer; Be combined into prevention oncogene mutation and the compatibility unit of killing cancerous cell by different level, capture the intractable of cancer; To be enough to the killing cancerous cell effective dose, control the development of tumor, solve the rapidity that cancer changes.Finally obtain tumor cell and cancerous cell effective percentage and kill rate all at the prescription more than 99.9%.
2. treatment as claimed in claim 1 and prevention tumor prescription; The preparation production technology that it is characterized in that example Cordyceps tumor recovering side oral liquid; Earlier with the ethanol of 15-95% to Cordyceps or Cordyceps mycelium; Or medicine food dual purpose plants such as Fructus Ligustri Lucidi, papaw leaf are extracted, obtain part micromolecule effective ingredient after, with the raw material slag of alcohol extraction again water extract.Its extract carries out membrane filtration step by step by the molecular weight size, and the clarification back is directly concentrated, dry, promptly gets the effective ingredient of described treatment and prevention tumor.
3. preparation technology according to claim 2 is characterized in that, said alcohol with the consumption of the unit of being upgraded to be with the kilogram be unit quantity of material such as Cordyceps mycelium 6-20 doubly, extraction time is 1-2 hour, extraction time is 1-3 time.
4. preparation technology according to claim 3 is characterized in that, the residue after the alcohol extraction is heated to 50-100 ° of C continuous extraction 2-3 time with the water of 6-20 times of weight, extracts 2-3 hour, to obtain the extract of various effective ingredient at every turn.
5. according to the described preparation technology of one of claim 2-4; It is characterized in that; The extract of the various effective ingredient that obtain carries out membrane filtration from big to small successively according to molecular weight; Do not obtain through part and clarify again, concentrate, then can directly rare liquid preparation that is made into according to the prescription requirement, or employing vacuum drying, spray drying, lyophilization are processed powder.
6. like the application of effective ingredient such as the described Cordyceps mycelium of claim 2-5, Fructus Ligustri Lucidi; It is characterized in that; This effective ingredient except that the demand that satisfies the present invention prescription, also can with other Chinese medicine and western medicine or food compatibility, be used to prepare the medicine and the functional health-care food of treating other disease.
7. the application of effective ingredient according to claim 6; It is characterized in that; This effective ingredient or comprise the medicine and the combinations of foods of this effective ingredient is processed capsule, pill, tablet, powder, injection, granule, oral liquid, syrup, unguentum, medicated wine, medicinal tea, piece agent, electuary and beverage.
8. prescription as claimed in claim 1, the oncogene mutation that wherein said cancer is brought out by AFB1 (AFB1), DMBA, benzopyrene, urethanes, AOM (AOM), free radical, cytosol APC Protein kinase C (PKC), after birth LCK (TPK), cyclophosphamide, N-N-nitrosodimethylamine, myristoyl Buddhist skin alcohol ethyl ester (TPA), the smoke from cigarette factors such as (CSC) causes or is caused by retrovirus.
9. prescription as claimed in claim 1, wherein said cancer are pulmonary carcinoma, hepatocarcinoma, gastric cancer, breast carcinoma, intestinal cancer, uterus carcinoma, esophageal carcinoma, osteocarcinoma, bladder cancer, lymphatic cancer, nasopharyngeal carcinoma, cancer of pancreas, skin carcinoma, the brain cancer, leukemia etc.Its example is pulmonary carcinoma and rectal cancer.
10. compatibility prescription as claimed in claim 1, effective ingredient that this compatibility prescription contains and pharmacy, health food aspect acceptable carrier.
11. example as claimed in claim 2; It is characterized in that " the Cordyceps tumor health " title in the example " Cordyceps tumor recovering side oral liquid " comprises capsule, pill, tablet, powder, injection, granule, syrup, unguentum, electuary etc.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013174812A1 (en) * | 2012-05-22 | 2013-11-28 | Nestec S.A. | Fructus ligustri lucidi for bones in young subjects |
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| CN113304164A (en) * | 2021-06-11 | 2021-08-27 | 朱峰 | Application of kaempferitrin in preparation of non-small cell lung cancer resistant medicine |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013174812A1 (en) * | 2012-05-22 | 2013-11-28 | Nestec S.A. | Fructus ligustri lucidi for bones in young subjects |
| CN105833245A (en) * | 2016-05-03 | 2016-08-10 | 刘炳慧 | Pharmaceutical composition for hepatic adenoma postoperative coordinating and preparation method thereof |
| CN113304164A (en) * | 2021-06-11 | 2021-08-27 | 朱峰 | Application of kaempferitrin in preparation of non-small cell lung cancer resistant medicine |
| CN113304164B (en) * | 2021-06-11 | 2022-04-08 | 朱峰 | Application of kaempferitrin in preparation of non-small cell lung cancer resistant medicine |
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