CN102319255B - Anti-radiation spriulina polysaccharide organic bio-active iodine and application thereof - Google Patents
Anti-radiation spriulina polysaccharide organic bio-active iodine and application thereof Download PDFInfo
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- CN102319255B CN102319255B CN2011101512893A CN201110151289A CN102319255B CN 102319255 B CN102319255 B CN 102319255B CN 2011101512893 A CN2011101512893 A CN 2011101512893A CN 201110151289 A CN201110151289 A CN 201110151289A CN 102319255 B CN102319255 B CN 102319255B
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Abstract
The invention provides spriulina polysaccharide organic bio-active iodine and application thereof. The spriulina polysaccharide organic bio-active iodine is obtained by adding spriulina polysaccharides and organic bio-active iodine into fresh vital alga mud serving as a carrier and undergoing an optical autotrophic reaction under illumination and temperature control. The spriulina polysaccharide organic bio-active iodine can be used for preparing medicaments and health-care foods which are resistant to ionization and micro nuclear radiation, or nuclear radiation, cancers or viruses and can be used for enhancing the human immunity.
Description
Technical field
The present invention relates to a kind of anti-radiation spriulina polysaccharide organic bio-active iodine and application thereof.
Background technology
British scientist Bei Fuli once foretold " threatening human two large stealthy killers is radiation and overfatigue ", the mankind are in the in various degree electromagnetic radiation scopes of appliance communication such as mobile phone, refrigerator, TV, computer, daylight lamp, microwave oven, electromagnetic wave, air-conditioning, vacuum cleaner, add the radiation that produces in the Nature, after time is permanent, cause the brain injury, cause the symptoms such as fatigue, tinnitus, ophthalmic, headache feeling of fullness in the head.
" World Health Organization claims to use for a long time the careful usefulness of the mobile phone carcinogenic suggestion teenager of possibility "
Source: on 06 01st, 2011 15:25 of China News Service
The subordinate's of World Health Organization (WHO) on the 1st IARC united and delivered report and claim that certain cancers (particularly glioma) may be related with mobile phone www.chinanews.com's June, and glioma is a kind of of the brain cancer.
The president Obama cancer member Sa Mite of consultative committee of country (Jonathan Samet) says in statement: " may be risky, we need to further observe the contact of mobile phone and cancer.”
When using mobile phone, the electromagnetic wave that gives off can heat tissue, and damages.World Health Organization (WHO) and multinational government are all ben to be, teenager should carefully be used mobile phone.Teen-age ear and skull are less, thinner than the adult, and they are when using mobile phone, and the radiation that brain absorbs will exceed 50% than the adult.
On April 26th, 86, former Soviet Union's Chernobyl Plant blast.1979, U.S.'s Three Mile Island Nuclear Station accident.Violent earthquake on March 11st, 2011 causes the blast of Fukushima, Japan nuclear power station, involves the whole world.Radioactive substance is discharged into the atmosphere in nuclear blast, forms the radioactivity plume, along with the air-flow drift; The radioactive substance cooling water is discharged in the ocean, and near national vegetable and tap water have detected micro-radioactive substance, and up to the present micro-radioprotective 100 little west not are difficult to provide final conclusion to Health Impact." sickness rate and amount of radiation are linear, extend to the low dosage scope always for radioprotective expert Deng Puyan." German nuclear power station disclosed at " European cancer journal " 2008 years, lived in around the nuclear power plant in the 5000m scope, child below 5 years old suffers from the carninomatosis rate and rises 47%.Xinhua News Agency's telecommunication report, after the expert thought 30 years, the Fukushima radiation will diffuse to the whole Pacific Ocean.Another nuclear energy expert Robert Brenner thinks that " radiation destruction DNA, and that I guess damaged cell all may become the cancer seed, the development of cancer can last many decades." micro-radioprotective is that slow 10 years and even 20 years are carcinogenic to human health, outburst out before is not a stealthy killer who hides for a long time and can not neglect.
Nuclear blast radiation 131-iodine by media report after, many people take for inorganic iodine and can resist nuclear blast, cause at home panic buying iodine salt agitation (in fact 5 kilograms of disposable taking of the total content of iodine of iodine salt could be resisted nuclear blast 131-iodine), human blast produces frightened to nuclear energy." Pandora's box " phenomenon has occured, and nuclear energy power generation promotes the well-being of mankind simultaneously, brings on a disaster to the mankind again.
" spirulina radioprotective anticancer function and principle (one) "
Select from spirulina radioprotective anticancer function and principle (one) Baidu library
Also for it is found that, this paper is concise and to the point comprehensive to spirulina research work one for the radioprotective of spirulina, antimutagenic, anti-cancer, antitumaous effect.
Research data shows that spirulina has very strong radioprotective and antimutagenic, irradiation with radiation spirulina with different radians, when exposure rate reaches 100Gy (100k rad), all do not observe the inhibitory action to growth of spirulina platensis, illustrate that spirulina has very strong radioresistance.
The cyanophycear microalgae of Spirullina just has the people to find that the extract of lyngbya majuscula has anti-leukocythemia liveness in the cyanophyceae before very early, thereby has evoked the research interest of people to cyanophyceae.
Liu Lisheng etc. (1991) have done with the research of spirulina polysaccharide to the effect of transplantability inhibition of cancer cell, spirulina polysaccharide can significantly suppress the fermented soya beans, salted or other wise that increases of osteosarcoma cell in the Mice Body when the 200mg/kg agent is, suppress the synthetic of ascites hepatoma cells DNA and protein.Inhibitory action in 3-24 hour in time prolongation and improve, by half amount of suppression computational analysis to different JEG-3, illustrate that different two cells are different to the sensitivity of spirulina polysaccharide.After medicine removed from culture medium, the synthetic speed of cancerous cell DNA was recovered rapidly.Illustrate that spirulina polysaccharide is to finish by suppressing the synthetic institute of DNA to the inhibition of cancer cell multiplication, and this inhibition is to belong to metabolic inhibition.
" research of spirulina radiation resistance " Cui Wenming, Zhang Xin, Liu Zeqin, Li Ning, Han Chi (Chinese Academy Of Preventive Medicine Research Institute Of Nutrition And Food Hygiene, Beijing 100050)
This experiment is take Leukocyte Counts, 30d mouse survival rate, Micronucleus and mouse's germ cell chromosome aberration rate as observation index, and the anti-radiation health care effect of spirulina is studied.
It is 4.8g 60kg that the spirulina human body is recommended consumption
-1d
-1Kunming mouse (secondary) and standing feedstuff are provided by Laboratory Animal Science section of Beijing Medical University.The moving word 01-3049 of qualified number doctor of animal.
X ray irradiation x 1 time, radiation parameter close rate are 3.21Gy/min, and radiated time is 2min 53s, and exposure dose is 9Gy.
The result shows that caused mouse chromosome damage has protective effect to spirulina to multi irradiation, and is preferably dose-response relationship.
The result shows that spirulina has protective effect to the caused sexual cell chromosome damage of inferior chronic irradiation.
" spirulina radiation resistance " (professor of biomedical research institute of Qi power Peking University, the head of the department of internal medicine of Affiliated Hospital of Beijing Medical University, FAMA are published in March 21 " Liberation Daily " in 2003)
Spirulina radioprotective mechanism
Contain 5 times of beta-carotene to Radix Dauci Sativae, phycocyanin and polysaccharose substance in the spirulina.Human free radical increases and damaging cells causes cancer, and beta-carotene is one of establishment free radical activity material.Algae protein, algal polysaccharides are improved immunologic function for domestic and international medical circle is generally acknowledged, and inhibition or killing tumor cell have sure curative effect.Simultaneously the side effect leukocyte increasing of ameliorate tumor after chemotherapy, radiotherapy all there is precise effects.The opposing ray has stronger radiation resistance to the myelocytic infringement of human bone.And the gamma-Linolenic acid that contains, vitamin E and superoxide dismutase (SOD) can strengthen cell viability.And at occurring in nature, gamma-Linolenic acid only just contains in spirulina and breast milk.
Ruan Jihong studies show that, spirulina polysaccharide and GL-PP can significantly alleviate and improve aberration rate and chromosome aberration type that radiation-induced Vicia faba Root Tip Cells is examined.Can obviously reduce after the radiation micronuclear rates of polychromatic erythrocyte in the mouse bone marrow cells, keep the ratio of medullary cell PEC/NCE, and radiation-induced leukopenia is improved significantly.Thereby think that spirulina polysaccharide and GL-PP are a kind of effective natural radiation protective agents.
I is the technical field of two kinds of different subjects from spirulina polysaccharide, and depending merely on spirulina polysaccharide can not be to Antiradiation, and International Atomic Energy Agency of World Health Organization assert that available iodine could Antiradiation (spirulina polysaccharide is not identified Antiradiation).The rich iodine polysaccharide of dark star Times report Zhao Bo on May 2nd, 98 superman Spirulina powder is identified (seeing Appendix) by Shenzhen Municipal Government.People's Daily's 29, scientific and technological world column in 98 on June the 15 edition has been reported Shenzhen Municipal Government and science and technology office's expert's conclusion: the rich iodine polysaccharide of superman's spirulina is high temperature resistant, without the algae raw meat, causes the huge repercussion of domestic and international scientific and technological circle.It is an innovation that organic iodine and spirulina polysaccharide combination are added in the food, has filled up domestic and international blank.
Spirulina polysaccharide is a kind of biomacromolecule that extracts from spirulina, function with radioprotective, mutation, propagation capable of inhibiting cell, and having the character such as the expansion very similar to dietary fiber, gelatification, moisture holding capacity, low heat value, this has showed wide prospect for its application in functional health product.
Spirulina polysaccharide (PSP) is a kind of existing conventional biological extraction technology, and obtainable anticancer antineoplastic bioactive substance extracts with the trichloroacetic acid method of improveing, and products obtained therefrom polysaccharide mass content reaches more than 92%, is better than traditional SEVDG method.Spirulina polysaccharide has good medical medical value.The first radioprotective anti-aging effects, PSP significantly enhanced rad causes DNA damage resurrection and unscheduled DNA synthesis, and animal bone marrow cell regeneration ability after delaying the saturated of above two important reparation reactions and suffering radiation, promote the recovery of hemopoietic system raying damage, have certain radioprotective and chemically resistant material effect, have the inhibitory action of killing and wounding to cancerous cell; Second improves immune function of human body; The 3rd antivirus action.Expensive because of spirulina polysaccharide, China's medical circle is mainly only for being used for anticancer antitumor.Existing product, Shenzhen enterprise delivers to the outer space with the algae kind by spacecraft, breeding goes out " space spiral algae sheet ", " space breeding " method improves spirulina polysaccharide content, antagonism light current magnetic radiation and chemotherapy have certain effect, but can not be effectively to Antiradiation " iodine-131 ", and cost be expensive.At present prior art has the safe iodine " potassium iodide tablet " of kind of Antiradiation to place restrictions on when the nuclear energy crisis occurs under given conditions to use.
International Atomic Energy Agency of World Health Organization (WHO) has proposed " Stable Iodine preventative strategies can under the nuclear energy case of emergency ", its Stable Iodine is real to be inorganic iodine tablet (potassium iodide tablet and potassium iodate sheet), and to announce be not that " radiation antidote " neither everyone can both be taken, only under NSF clearly instructs just needs can take.
U.S. FPA is decided to be the GRS level to potassium iodide and potassium iodate, belongs to low toxicity material, adds Sal and go up century-oldly, becomes world today's iodine salt that the whole world of specializing in must eat that " enriches the iodine ".
World Health Organization announces the present whole world 6.5 hundred million population goiter, malpractice, it is in rising trend to suffer from papillary thyroid carcinoma, in the areas such as non-, Southeast Asia, the Central Asia, Central Europe, Eastern Europe spread unchecked.
205 thyroid carcinoma patients received in 1 year in south of the River Urban Newspaper on April 23rd, 2010 " our salt overfeed " Zhejiang Hospital, increased by 10 times before 10 years.All owing to inorganic iodine salt causes.
Antichemical troop of the military all has highly enriched effective iodine tablet (potassium iodate sheet and potassium iodide tablet) when antagonism chemical and biological weapons and bacteriological warfare both at home and abroad.
In view of inorganic iodine belongs to low toxicity material, it has dual nature, and it can resist nuclear blast, but the people is known from experience the injury that causes in various degree.How to substitute inorganic iodine antagonism nuclear blast with organic iodine and just become our this generation scientific worker urgent need to solve the problem.
Mr. Zhao Bo just will be rich in the organic iodine polysaccharide before the more than ten years Spirulina powder has been made the blue polysaccharide extraction liquid of algae by biotechnology, add in the food such as soy sauce, monosodium glutamate, and the blue polysaccharide extraction liquid of algae added in Tsingtao beer and the Maotai, cause that food circle causes a sensation.
" the safe iodine " of World Health Organization (WHO) (potassium iodide tablet and potassium iodate sheet) radioprotective principle is " attacking large poison with mild toxicity " method, after taking the potassium iodide tablet and potassium iodate sheet of super excess iodine, thyroid is full of, can resist radioiodine (131 iodine) and invade human body, prevent that thyroid carcinoma from occuring.Its unfavorable factor and drawback are attended to one thing and lose sight of another, and be hard to guard against.Adopt this measure, relatively can reduce " thyroid carcinoma " sickness rate, cause from now on thyromegaly poisoning and canceration wind-transformation danger but can not reduce.Chinese public health the 4th phase of the 22nd volume April in 2006 " excessive iodine is to the development and use of body harm and organic iodine " has been reported, other has research to find, inorganic iodine is taken in and is surpassed 40 μ g/d, the obvious enlargement of Mouse thyroid behind the 180d, even and Thallus Laminariae (Thallus Eckloniae) organic iodine intake surpasses 200 μ g/d, Mouse thyroid also has no obvious enlargement, and it is swollen to find also that simultaneously the Thallus Laminariae (Thallus Eckloniae) organic iodine can be prevented and treated high iodine first.
Chinese public health the 4th phase of the 23rd volume April in 2007 " iodine is on the impact of human thyroid cell proliferation " report, the sickness rate of China's thyroid disease presents the rising stage variable concentrations trend, too much inorganic iodine can suppress the propagation of thyroid cell, the infringement thyroid cell, and organic iodine is less on the impact report of thyroid cell propagation situation.This paper utilizes external iodine irritant test to measure the organic iodine of variable concentrations and inorganic iodine to the impact of thyroid cell propagation situation, inquires into organic iodine on the impact of human thyroid cell, for it provides foundation as a kind of safe preparation that enriches the iodine.
Mr. Zhao Bo does experiment with own human body, takes continuously the organic iodine of 5 years high doses, and body burden reaches millions of micrograms, and its urine iodine has reached 7950 micrograms, and normal person's urine iodine is 200 micrograms, sets the world record.(seeing Jiangxi Province health and epidemic prevention station 98 years urine iodate verification certificate for details) if chemical inorganic iodine will certainly die undoubtedly, the safety of organic iodine to human body of having given counterevidence.Press the health ministry required standard with spirulina polysaccharide and organic bio-iodine, can be mixed with the organic iodine tablet of Antiradiation of new generation, the inorganic iodine tablet that replaces Antiradiation provides technical support.(seeing embodiment five for details)
" Chongqing Medical " 03 phase in 2003 is published " the antagonism impact that spirulina platensis polysaccharide suppresses radiation induced mice hemopoietic function " clinical trial, and unite common experiment results proved by hospital general, Tibet Military Area Command department of dermatologry, anti-former medical science teaching and research room of preventive medicine system of Third Military Medical University and large level ground hospital blood internal medicine, biology department of Nanjing University: spirulina polysaccharide can be to Antiradiation.The hematopoietic cell culture technique is used in this experiment, selects blunt top polysaccharides (SPP), systematically observed SPP to normal mouse and
60Co gamma-rays 5Gy irradiation mice periphery detailed information born of the same parents, impact and the effect characteristics thereof of marrow hemopoietic stem cells (CFU-S) and hemopoietic progenitor cell (CFU-GM, CFU-E, BFU-E) proliferation and differentiation are intended at the regulating and controlling effect of research spirulina polysaccharide to the body hemopoietic function.
Healthy Kunming mouse, male and female half and half, in Mus 8-10 in age week, body weight 22-24g is provided by Third Military Medical University's Experimental Animal Center.The irradiation of illuminate condition 60Co γ 8Cy lethal dose, absorbent rate rate 46.75cGy/min, be used for the hematopoietic stem cell colony and measure, 5G master's sublethal irradiation is for detection of the experiment of peripheral blood various types of cells and grain-monosystem hemopoietic progenitor cell, hemopoietic progenitor cell of red blood cell line and nucleated marrow cell population.
The result: mouse peripheral blood WBC, PLT and the equal compared with normal matched group of BMC obviously reduced (P＜0.01) after the impact of spirulina platensis polysaccharide human peripheral blood cell and skeleton number of nucleated cells was hindered, radiation medication group mice WBC, BMC are again apparently higher than the radiation matched group, and Hb, PLT count medicated cap without obviously changing (P＞0.05).Table 1 shows positive herbal companion mice BMC apparently higher than Normal group (P＜0.01), and other index is compared with Normal group, difference that there are no significant (P＞0.05).
See adnexa for details: (x ± s), table 2 spirulina platensis polysaccharide is on the impact of mouse hemopoietic CFU-GM and hematopoietic stem cell number (x ± s) on the impact of mouse peripheral blood cell and bone marrow nucleated cell number for table 1 spirulina platensis polysaccharide.
SPP can promote propagation and the differentiation of hematopoietic cell, participates in the direct or indirect regulation and control activity of hemopoietic progenitor cell and hematopoietic stem cell, and hemopoietic and immune group are woven with material impact.This impact may be again the main cause that SPP has radiation resistance and inhibition cell proliferation.
The blast of former Soviet Union Chernobyl Plant, maximum in the Japanese disaster relief supplies is exactly spirulina capsule ball and the tablet that contains polysaccharide.
The disease prevention and control center, Jiangxi to " salt with rich iodine spirulina " (namely containing pure organic bio-iodine) total iodine in 84700 micrograms, mouse toxicological experiment report (December in 2009 complete inspection on the 1st) serve on the conclusion after 30 days, it three causes test and all belongs to negative, the PCEMNR micronucleus test, the mouse sperm deformity test, the AMES test.Main organs is dissected without pathology and is changed, and true border is nontoxic.(looking into newly through retrieval, both at home and abroad without this case) and inorganic iodine (potassium iodate, potassium iodide) are namely died after the mouse food when 10000 micrograms-10140 microgram.The safety of sufficient proof organic bio-iodine is far superior to potassium iodide, potassium iodate.
Organic bio-iodine is again tyrosine iodine, wherein 2-Amino-3-(4-hydroxy-3-iodo-phenyl)-propionic acid content 96%, 2-iodotyrosine content 2%, sodium alginate 2%.Wherein the 2-Amino-3-(4-hydroxy-3-iodo-phenyl)-propionic acid chemical structural formula is:
2-iodotyrosine content 2%, structural formula is:
Organic bio-iodine and advantage thereof are " organic bio-iodine and application thereof " (patent No.: ZL200310111222.2, patentee: set forth Zhao Bo).
Summary of the invention
Purpose of the present invention just provides a kind of anti-radiation spriulina polysaccharide organic bio-active iodine, do not occur in the nuclear energy crisis or the nuclear energy accident has occured after strick precaution and the prevention of the micro-radioprotective that produces of surrounding area.Use the product of the present invention's preparation that micro-radioprotective is had outside strick precaution and the preventive effect, can effectively resist control and cause radiation to human body after all kinds of mobile phones, computer and household appliances and the chemotherapy, reach resisting fatigue, antiviral, anticancer change improves immune function of human body, effectively enriches the iodine, without any side effects, improve human health quality; The present invention in being about to the nuclear energy accident to occur and the nuclear energy accident occurs for the mankind effectively to Antiradiation and all kinds of different radiation, the prevention effect that is better than prior art " Stable Iodine " (being potassium iodide tablet and potassium iodate sheet) is provided, its radioprotective principle is " attacking large poison with nontoxic ", has novelty, creativeness.The health of bringing benefit to the mankind.
The present invention is achieved like this:
A kind of spirulina polysaccharide organic bio-iodine is provided, it is characterized in that take new living body algae mud as carrier, add spirulina polysaccharide and organic bio-iodine, issue third contact of a total solar or lunar eclipse autotroph reaction and obtain the spirulina polysaccharide organic bio-iodine in illumination and temperature control.
Wherein, spirulina polysaccharide organic bio-iodine described above by weight ratio, is preferably algae mud and accounts for 50-96%, and spirulina polysaccharide accounts for 3-50%, and organic bio-iodine accounts for 0.0001-10%.
Spirulina polysaccharide organic bio-iodine described above is characterized in that described new living body algae mud is selected from spirulina plalensis, huge spirulina or chlorella.
Spirulina polysaccharide organic bio-iodine described above is characterized in that described organic bio-iodine is comprised of 96% 2-Amino-3-(4-hydroxy-3-iodo-phenyl)-propionic acid, 2% 2-iodotyrosine and 2% sodium alginate.
Spirulina polysaccharide organic bio-iodine described above is characterized in that described illumination is daylight or artificial light sources, its intensity of illumination 5-10 ten thousand luxs, wavelength 400-700 millimicron.
Spirulina polysaccharide organic bio-iodine described above is characterized in that described temperature control is that temperature is between 30 ℃-40 ℃.
Spirulina polysaccharide organic bio-iodine described above is characterized in that calculating with the dry powder of algae mud, and every 100g algae mud dry powder adds spirulina polysaccharide 3-50 gram, and organic bio-iodine calculates 100-10 with iodine, 000,000 microgram.
The present invention also provide spirulina polysaccharide organic bio-iodine described above for the preparation of radioprotective, improve the application in body immunity, anticancer or antiviral medicine or the health food.
Wherein, application described above, described radiation are ionizing radiation and examine micro-radiation or the nuclear energy radiation.
Particularly, from spirulina plalensis, in huge spirulina or the chlorella culturing pool → fish for fresh algae mud → mistake and fill in → put into container (or another algae pond) → batching: take algae mud as carrier, algae mud requires the 10-15 kilogram by the technical standard of 1 kilogram of algae powder of dry reducible one-tenth, spirulina polysaccharide after add purifying in proportion and pure organic bio-iodine powder (patent of invention number: ZL2003 1 0111222.2) → stir simultaneously → at daylight or artificial light sources, intensity of illumination 5-10 ten thousand luxs, wavelength 400-700 millimicron, occur between 30 ℃-40 ℃ of the temperature controls optics autotroph course of reaction → total content of iodine of detections organic iodine (calculating effectively from 5 micrograms-100,000 microgram with every gram dry powder content) and spirulina polysaccharide content (contain 3-50 take per 100 gram dry powder and restrain polysaccharide as effective standard) up to standard after, spray drying or lyophilization → finished product packing → 15 ℃ of-20 ℃ of storage at a constant temperature of warehouse-in.
Spirulina polysaccharide of the present invention can prepare by the known spirulina polysaccharide preparation technique of prior art.For example:
A, first spirulina powder is carried out broken wall treatment when slightly extracting spirulina polysaccharide (polysaccharide of Spirulina.PS), remove low-polarity component with low polar solvent, carry (can adopt microwave-assisted to extract) through hot-water extraction or alkali liquor cold-draw, get supernatant concentration, centrifugal behind the alcohol chromatography, the dry spirulina crude polysaccharides that gets.Protein accounts for 60～70% of dry cell weight in the spirulina, and therefore extracting the spirulina polysaccharide key is effectively to remove protein.
At present, the method for Deproteinization mainly contains following several: Sevag method, foam separating technology, trichloroacetic acid (TCA) method, heating concentration method, enzyme process.The Sevag method is the classical way of deproteinize, usually is only applicable to except a small amount of albumen, and must be repeatedly.TCA method Deproteinization can shorten flow process, and the polysaccharide loss rate reduces, and is conducive to follow-up purification.Improve the yield of polysaccharide crude product with enzymatic degraded Intake Protein mass-energy, but still have the protein more than 30% in the gained composition.Adopt two enzymes method (trypsin, papain) to carry out polysaccharide extracting process, recording residual protein content is 3%.
B, purification
May contain a plurality of mixed sugar components and small molecular weight impurity by the resulting crude polysaccharides of water extraction liquid extracting, further grading purification just can obtain the single sterling of spirulina polysaccharide.Generally to observe single narrow peak behind the chromatography as the standard of Purity.Purification process commonly used has:
(1) ion-exchange cellulose chromatography: ion exchange and cellulose chromatography combine to be made a series of ion-exchange cellulose chromatographies to be based on resin different to the affinity of different ions, and the various polysaccharide of stripping are for separating of saccharide.Often adopt NaCl solution or phosphate buffer solution gradient elution in the separation process, can obtain the one-component of different fractions.Common are DEAE cellulose and DEAE-Sepharose.
(2) gel filtration: the polysaccharide goods need further just can obtain the homogeneous polysaccharide sterling with the gel filtration purification after the DEAE cellulose separates.Gel filtration is that the gel particle that a kind of usefulness has a certain pore size size is the chromatography method of holder, and separable molecule with different size and shape is effective especially for separating of the saccharide of different polymerization degree.Polydextran gel (Sephadex G), agarose gel (Sepharose), polyacrylamide gel (Bio-gel P) is hydrophilic gel, all is widely used in the purification of polysaccharide.General Sephadex G100 or Sephadex G200, the Sepharyl S100 of adopting in the purge process of spirulina polysaccharide.But Sephadex G itself is saccharide, is stained with the sugar that elutes from gel unavoidably in eluent, may cause the impure of refining polysaccharide.Measurement of the polysaccharide content detects with sulphuric acid anthrone method root track behind the purification.
The present invention also provides a kind of more efficient spirulina polysaccharide novel technology for extracting.Namely adopt two enzymes method (trypsin, papain) to carry out polysaccharide extracting process, the deproteinization effect is near 100%, and crude polysaccharides adopts ion-exchange chromatography and gel chromatography to be further purified, and surveys its composition by paper chromatography.By DEAE-Sep harose and Sep hadex-G50 purification of crude polysaccharide, obtain 2 polysaccharide components.The monosaccharide of component 1 consists of D-Portugal glucose, D-xylose, D-galactose, glucuronic acid after measured; The monosaccharide of component 2 consists of: D-Glucose, D-MANNOSE, L-rhamnose, D-galactose, glucuronic acid.
The algae powder solution → trypsin hydrolyzing of Spirulina powder → be made into 12% → papain hydrolysis → vacuum concentration → alcohol precipitation → washing with acetone → lyophilization → polysaccharide semifinished product polysaccharide semifinished product → ion-exchange chromatography → dialysis → gel chromatography → lyophilization vacuum concentration: at 70～80 ℃ of vacuum concentration to containing solid content 20%.Alcohol precipitation separates: add 95% ethanol of 5 times of volumes in concentrated solution, make spirulina polysaccharide be flocculent deposit and separate out, most of protein and other composition are retained in the solution, and the centrifugal 15min of 4000r/min must precipitate.
The triumphant formula nitriding of protein determination.
Determining of trypsin optimum temperature, tryptic optimum temperature is 55 ℃, this moment, enzymolysis deproteinization effect was best.
Determining of trypsin Optimun pH, trypsin has higher enzyme activity in 6.2～7.2 scope.
Determining of trypsin digestion time, behind the trypsin acting 2.5h in the polysaccharide crude product protein content no longer include obvious decline, but so stopped reaction behind the protease effect 2.5h.
Trypsin is determining of the suitableeest actuating quantity, and the deproteinization effect no longer obviously increased after enzyme dosage reached 3.0%, so enzyme dosage is defined as 3.0%.
Utilize papain to carry out the secondary hydrolysis
Enzyme concentration 3%, in the buffer of pH6.5,40 ℃, insulation 20h, often jolting.
Enzymolysis solution is behind vacuum concentration, alcohol precipitation, and recording residual protein content is 3%.
The ion-exchange chromatography separating polyose
(20 * 1cm) carry out separation of polysaccharides: the pyridine of usefulness 0.01mol/L, p H8.0-hydrochloride buffer equilibrium ion exchange column to adopt DEAE-Sep harose post, the applied sample amount of crude polysaccharides is 8mL, flow velocity is 25mL/h, carry out gradient elution with pyridine-hydrochloride buffer of 0.01mol/L, p H8.0 and the NaCl of 0～2mol/L, get curve shown in Figure 1.Two peaks of this curve are collected respectively, and lyophilization gets thick component 1 and thick component 2.
The gel chromatography separating polyose
(90 * 2cm) carry out separating of the thick component 1 of polysaccharide and the thick component 2 of polysaccharide, and eluent is distilled water, and flow velocity 25mL/h gets Fig. 2, curve shown in Figure 3 to adopt Sep hadex-G50 gel column.Collect respectively single solution, get polysaccharide pure component 1 and pure component 2 after the lyophilization.
The polysaccharide sample gets monosaccharide and consists of through acidolysis, paper chromatography: component 1 is D-Glucose, D-xylose, D-galactose, glucuronic acid; Component 2 is D-Glucose, D-MANNOSE, L-rhamnose, D-galactose, glucuronic acid.
Spirulina polysaccharide organic bio-iodine of the present invention can be used as edible and medical, can produce the series of products such as anti-radiation spriulina polysaccharide organic bio-active iodine sheet, capsule, granule ball, instant powder, oral liquid, volumetric solution.
Organic iodine content in the product of Antiradiation of the present invention adds according to the up-to-date specified value of China's Ministry of Public Health to be made, 25 milligrams of children below 3 years old (i.e. 25000 micrograms) wherein, be grown up 100 milligrams (i.e. 100000 micrograms) pregnant and lying-in women and 3-12 year 50 milligrams of children (i.e. 50000 micrograms); 100 milligrams of food rations of being grown up are equivalent to five kilograms of iodine salt total iodo quantities.
In the above-mentioned Antiradiation product of preparation, can adopt existing medicine microcapsule production technology and existing iodized oil ball production technology.Wherein, with the walnut oil in the former iodized oil ball and Oleum Glycines deletion, change into and use olive oil (its unsaturated fatty acid content of fine quality is high), be of value to the care of heart and blood vessel, can reach the purpose that human body slowly absorbs available iodine, in the hope of effective and safe more to radioprotective iodine.Be about to occur above-mentioned disposable food amount to be mixed with dose 2-3 time within front 24-48 hour for nuclear energy, replace the slow releasing agent effect.
The micro-radiation of anti-nuclear, ionizing radiation-resistant and chemotherapy product contain the natural spiral seaweed polysaccharide at 3-12g, and its organic iodine content versatility product contains the preparation of 5 micrograms by every gram, is fit to any crowd.For increasing anticancer antitumous effect, spirulina polysaccharide content is at 12-50g.
Radioprotective also has caesium-137, nitrogen-16, tritium except iodine 131, radioactive substance also can damage human body, the uniting antagonism and can reduce it to human injury's degree of organic bio-iodine and spirulina polysaccharide.Organic bio-iodine and spirulina polysaccharide not only fill in the thyroid under excessive state, also are covered with in the Whole Body fatty acid to reach in the blood, play the effect of an amount of protection skin, skeleton, blood, the five internal organs.Anti-treating leukemia etc. there is certain effect.After adding spirulina leucocyan and chlorophyll, have toxin expelling re-scheduling metal and radiate the gonosome inner product and deposit the thing effect.
Spirulina polysaccharide organic bio-iodine of the present invention can be made Dietotherapy health food, is used for the micro-radiation of anti-nuclear, ionizing radiation-resistant and chemotherapy and uses and improve body immunity and anticancer antiviral functions.
Spirulina polysaccharide organic bio-iodine of the present invention can be made medicine and use, be used for the radiation prevention of antagonism nuclear energy and treatment, rescue the victims of the disaster, reduce the canceration sickness rate, improve body immunity, the prevention effect that is better than prior art " Stable Iodine " (being potassium iodide and potassium iodate sheet), the health of bringing benefit to the mankind.
Spirulina polysaccharide organic bio-iodine of the present invention, detect A022 number report of the malicious searching of the anti-food of " superman's Spirulina powder " (rich iodine glycan) (98) Yue Wei according to acute toxicity test in mice, belong to nontoxic level material and the 2003CT00019 of Qingdao of Shandong province Center for Disease Control (CDC) " edible high iodine spirulina primary stability test " report, through No. 014 scientific and technological achievement assay certificate of State Scientific and Technological Commission's light section mirror word [199], assessment of result is made the food that enriches the iodine for it is added in Sal and other food, do not find like product at home and abroad, being an innovation, is a kind of safe food that enriches the iodine.Organic iodine salt was sold the city 11 years, and none routine toxic and side effects is highly commended by customers deeply.
Description of drawings
Fig. 1: DEAE-Sepharose post elution curve;
Fig. 2: the Sephadex-G50 post elution curve of polysaccharide component 1;
Fig. 3: the Sephadex-G50 post elution curve of polysaccharide component 2;
Fig. 4: SPP is to PBMC and K
562The cell toxic action;
Fig. 5: SPP incubation time and NK activity relationship.
The specific embodiment
Embodiment one: the micro-radiation of anti-nuclear, anti-household electrical appliances, communication, chemotherapy ionizing radiation, and spirulina polysaccharide organic bio-iodine compound method:
From the chlorella culturing pool, fish for new living body algae mud → mistake and fill in → put into container (or another algae pond) take algae mud as carrier, batching prescription conversion scale: can become 1 kilogram of algae dry powder after the spray-dried or lyophilization of the every 10-15 kilogram of its algae mud, add 30-500 gram spirulina polysaccharide by every 10-15 kilogram algae mud simultaneously, after adding simultaneously pure organic bio-iodine powder 1g and containing the 1-5 microgram → stir simultaneously → at daylight or artificial light sources (intensity of illumination 5-10 ten thousand luxs, wavelength 400-700 millimicron) between 30 ℃-40 ℃ of the temperature controls → after optics autotroph reaction → spray drying or lyophilization occur becoming spirulina powder, measure the per 100 gram content 100-500 micrograms of the total iodine of organic iodine, the per 100 gram content 3-50 grams of dry algae powder spirulina polysaccharide are qualified products → finished product packing → 15 ℃ of-20 ℃ of storage at a constant temperature of warehouse-in.
The said goods is radiation after the micro-radiation of anti-nuclear, anti-mobile phone, computer, household electrical appliances, the chemotherapy (this product is fit to any crowd to be taken, and not limited by the age-sex).
Embodiment two: the micro-radiation of anti-nuclear, anti-household electrical appliances, communication, chemotherapy ionizing radiation, and spirulina polysaccharide organic bio-iodine compound method (type that enriches the iodine, all ages and classes section amount is seen description):
From the chlorella culturing pool, fish for new living body algae mud → mistake and fill in → put into container (or another algae pond) take algae mud as carrier, batching prescription conversion scale: can become 1 kilogram of algae dry powder after the spray-dried or lyophilization of the every 10-15 kilogram of its algae mud, add 30-500 gram spirulina polysaccharide by every 10-15 kilogram algae mud simultaneously, after adding simultaneously pure organic bio-iodine powder 1g and containing the 20-50 microgram → stir simultaneously → at daylight or artificial light sources (intensity of illumination 5-10 ten thousand luxs, wavelength 400-700 millimicron) between 30 ℃-40 ℃ of the temperature controls → after optics autotroph reaction → spray drying or lyophilization occur becoming spirulina powder, measure the per 100 gram content 2000-5000 micrograms of the total iodine of organic iodine, the per 100 gram content 3-50 grams of dry algae powder spirulina polysaccharide are qualified products → finished product packing → 15 ℃ of-20 ℃ of storage at a constant temperature of warehouse-in.
Embodiment three: Antiradiation spirulina polysaccharide organic bio-iodine compound method:
From the spirulina plalensis culturing pool, fish for new living body algae mud → mistake and fill in → put into container (or another algae pond) take algae mud as carrier, the batching formula proportion: the every 10-15 kilogram of its ratio can become 1 kilogram of spirulina powder after lyophilization, add pure organic bio-iodine powder by every 10-15 kilogram algae mud simultaneously, make end product can reach adult's (every gram dry algae powder contains 100,000 microgram organic iodines) or pregnant and lying-in women and 3-12 year child's (every gram dry algae powder contains 50,000 microgram organic iodines), or child below 3 years old (every gram dry algae powder contains 2.5 ten thousand microgram organic iodines), after adding 300-500 gram spirulina polysaccharide by every 10-15 kilogram algae mud simultaneously → stir simultaneously, at daylight or artificial light sources (intensity of illumination 5-10 ten thousand luxs, wavelength 400-700 millimicron) between 30 ℃-40 ℃ of the temperature controls → after the optics autotroph occurs reacting → be spray dried to spirulina powder → measure the total iodine total amount of organic iodine, reach standard (adult, pregnant and lying-in women, child, World Health Organization (WHO) require the content regulation) be qualified → finished product packing → 15 ℃ of-20 ℃ of storage at a constant temperature of warehouse-in.If during batching, add phycocyanobilin and chlorophyll, namely have radiation deposit function in the toxin expelling isostere.
Embodiment four: Antiradiation spirulina polysaccharide organic bio-iodine compound method:
From huge spirulina culture pond, fish for new living body algae mud → mistake and fill in → put into container (or another algae pond) take algae mud as carrier, the batching formula proportion: the every 10-15 kilogram of its ratio can become 1 kilogram of spirulina powder after lyophilization, add pure organic bio-iodine powder by every 10-15 kilogram algae mud simultaneously, make end product can reach adult's (every dry algae powder contains 100,000 microgram organic iodines) or pregnant and lying-in women and 3-12 year child's (every gram dry algae powder contains 50,000 microgram organic iodines), child's (every gram dry algae powder contains 2.5 ten thousand microgram organic iodines) below 3 years old, after adding 300-500 gram spirulina polysaccharide by every 10-15 kilogram algae mud simultaneously → stir simultaneously, at daylight or artificial light sources (intensity of illumination 5-10 ten thousand luxs, wavelength 400-700 millimicron) between 30 ℃-40 ℃ of the temperature controls → after the optics autotroph occurs reacting → be spray dried to spirulina powder → measure the total iodine total amount of organic iodine, reach standard (adult, pregnant and lying-in women, child, World Health Organization (WHO) require the content regulation) be qualified → finished product packing → 15 ℃ of-20 ℃ of storage at a constant temperature of warehouse-in.If during batching, add a certain proportion of phycocyanobilin and chlorophyll, it is had accumulate the radiation residue in the toxin expelling isostere, from defecation, discharge.
Embodiment five: the anti-radiation spriulina polysaccharide organic bio-active iodine effect test:
40 times of superhumans
Zhao Bo excessive amount organic bio-iodine clinical report
One, purpose:
Use own health, do the absorption of human body experiment, prove that organic bio-iodine can be absorbed by the body; Do the experiment of human body excessive use organic bio-iodine, prove harmless.Making organic iodine tablet for Antiradiation reaches Ministry of Public Health required standard amount foundation is provided.
Two, test difficulty:
This pilot country is inside and outside to be " initiating ", and this similar test was not done by any detection or hygiene department, and who can not " test with body ", bears the personal safety and the accident responsibility that cause thus.
Three, guiding theory:
Do not find harm through edible high dose organic iodine over 5 years, the addition by test acquisition Ministry of Public Health provides foundation for being mixed with anti-nuclear blast organic iodine.
Zhao Bo takes 40 times of content of iodine verification certificates of superhuman analysis report (only for reference)
4 years edible accumulated doses: 1788500 μ g
Four, prepare before 40 times of content of iodine examinations of superhuman:
Five, 40 times of content of iodine of superhuman take the afterreaction observation:
Six, conjunctival congestion and constipation produce the analysis of causes and emergency measure:
The high concentration iodide enter in human body and the blood, cause that by blood circulation eye retina is congested, absorb peripheral moisture content, produce hyperemia and dry sensation: the high concentration iodide enter intestinal tract, because iodide have very big adsorptivity, intestinal wall and water adsorption are totally caused due to the constipation.
Its emergency measure, I gulp down drink large water gaging and popsicle, Mel and Fructus Musae, after test is finished, am to alleviate constipation, take a little " cow-bezoar anti-toxic bolus " in several days in JIUYUE after 15 days, Guangzhou five sheep boards " clearing moist heat " reach " summer Sang Ju Yin " electuary, namely recover normally.
Seven, survey report interpretation of result
Eight, above-mentioned test is understood
8.1 by taking continuously of the first four years, produced iodide ion up to a million in the body, rear six take microgram iodide ion every days 8000 continuously, have therefore produced side effect, withdraw rear side effect and disappear.
8.2 the experiment total amount of taking continuously six days is 54000 micrograms, meet Ministry of Public Health pregnant and lying-in women and 3-12 year child's (every gram dry algae powder contains 50,000 microgram organic iodines), child's (every gram dry algae powder contains 2.5 ten thousand microgram organic iodines) standard below 3 years old, this case has played the effect of alternative slow releasing agent.
8.3 because the overdose iodide, its urine iodine testing result is 7954 micrograms.(check on October 15th, 1 statement)
Embodiment six: salt is understood with rich iodine Spirulina powder (high dose group) radioprotective organic iodine:
This examining report list is provided by the disease prevention and control center, Jiangxi Province, sample number into spectrum: food 20091351, food 20092200.
Test basis: GB15193-2003 " food safety toxicology is commented program and method "
The toxicology check conclusion:
1, acute oral toxicity test: salt with rich iodine Spirulina powder to female male chmice acute Oral toxicity MTD all greater than 10g/kgBW, true border is nontoxic.
2, PCEMNR micronucleus test: the result is negative for the PCEMNR micronucleus test.
3, mouse sperm deformity test: the mouse sperm deformity result of the test is negative.
4, Salmonella reversion test: the Salmonella reversion test result is negative.
5,30 days feeding trials of rat: each dosage treated animal body weight, food utilization, main dirty body compare with the matched group comparing difference without significance (P＞0.05).The numeration of leukocyte of matched group and each dosage group and classification, red blood cell count(RBC), hemoglobin and every biochemical indicator (paddy the third Cyklokapren, glutami-oxaloacetic transaminase, blood urea nitrogen, creatinine, cholesterol, triglyceride, blood glucose, total protein, albumin) are all in this laboratory normal reference value.Each treated animal gross anatomy and main organs pathologic diagnosis are showed no significant pathological change.
Every mouse amount every day: 84700 micrograms, took continuously 30 days, totally 2541000 micrograms.Each treated animal gross anatomy and main organs pathologic diagnosis are showed no significant pathological change.Do not poison, pathological changes does not occur.Thus deducibility, the adult is each serving being safe with 100000 micrograms.Can reach Ministry of Public Health adult, pregnant and lying-in women, the anti-nuclear blast standard of children taking consumption with this dosage.Embodiment seven: spirulina platensis polysaccharide radioprotective effect test data
Table 1 spirulina platensis polysaccharide is on the impact of mouse peripheral blood cell and bone marrow nucleated cell number
Annotate: compare with Normal group,
△P＜0.05,
△ △P＜0.01; Compare with the radiation damage matched group
*P＜0.05,
*P＜0.01
Table 2 spirulina platensis polysaccharide is on the impact of mouse hemopoietic group cell and hematopoietic stem cell number
Annotate: compare with Normal group,
△P＜0.05,
△ △P＜0.01; Compare with the radiation damage matched group
*P＜0.05,
*P＜0.01
Embodiment eight: spirulina radioprotective effect test data
Table 1 spirulina pair
60The impact of Co-r irradiation Mouse bone marrow erythrocyte micronuclear rates
Compare a:p＜0.01, b: compare p＜0.005 with high dose group with matched group.
Table 2 spirulina pair
60The impact of Co-r irradiation mouse testis chromosome aberration of spermatocytes
Compare a:p＜0.01, b:p＜0.005 with the radiation matched group; Compare c:p＜0.001 with low dose group; Compare d:p＜0.01 with the blank group.
Embodiment nine: the spirulina platensis polysaccharide radioprotective is to the effect of Patients with Acute Leukemia NK cell
Referring to Fig. 4: SPP to PBMC and K
562The cell toxic action;
2-2-2SPP action time and NK cytoactive relation
10mgL
-1SPP add NK effector lymphocyte hatch different time on the NK cytoactive impact see Fig. 5, illustrate that the effector lymphocyte adds that the beginning produces effect more than the SPP effect 12h, 24h reaches higher level, is 24h so incubation time is selected in this experiment.
2-2-3SPP affects normal person NK cytoactive
PBMC of healthy people adds SPP10mgL
-1Rear NK cytoactive is 46.70 ± 10.56% no significant differences (p＞0.05) of comparing with normal person NK cytoactive, illustrates that this concentration SPP has no significant effect normal person NK cytoactive.
2-3SPP is to the effect of leukemia patient NK cytoactive
SPP makes 31 routine leukemia patient peripheral blood NK cell activity bring up to 38.33 ± 9.45% (p＜0.01) from 26.41 ± 10.17%, but still being lower than normal person NK cytoactive (p＜0.01), different stadium Patients with Acute Leukemia PERIPHERAL BLOOD MONONUCLEAR CELL add behind the SPP NK cytoactive all be significantly improved (p＜0.01).Though onset, recurrence phase patient NK cytoactive add and be improved behind the SPP but still be lower than normal person (p＜0.01), catabasis patient NK cytoactive adds behind the SPP the then no significant difference (p＞0.05) of comparing with the normal person, sees attached list.
Subordinate list SPP is to leukemia patient NK cytoactive
| The example number | Do not add SPP | Add SPP | The P value | |
| The onset recurrence phase | 23 | 20.30±6.72 * | 31.73±8.67 * | ＜0.001 |
| Catabasis | 8 | 30.64±8.91 * | 40.81±5.35 ** | |
| The P value | ＜0.01 | ＜0.01 |
Annotate:
*P＜0.01,
*P＞0.05 vs normal person
Embodiment ten: spirulina polysaccharide is to ultra-violet radiation resisting
3 results
3.1 the optium concentration of administration group: add medicine in 96 orifice plates, cell is cultivated, compare with matched group, each drug level group Growth of Cells differs, wherein 1600,800gL
-1Dosage group cell namely occurred the contracting of cell circle on the 2nd day and is difficult to sticking wall after inoculation, saw culture fluid inner cell residue on the 3rd day, and each drug dose group was carried out cell counting on the 4th day after inoculation, the results are shown in Table 1, thereby the dosage of determining PSP is 100,50,25gL
-1
The impact of the different PSP concentration of table 1 cell growth
| PSP concentration (gL -1) | n | Cell density (1 * 10 6Individual/mL) |
| 0 | 8 | 1.8±0.075 |
| 25 | 8 | 3.2±0.142 |
| 50 | 8 | 3.0±0.102 |
[0174]
| 100 | 8 | 3.0±0.149 |
| 200 | 8 | 1.4±0.127 |
| 400 | 8 | 0.8±0.093 |
The 3-2DNA injury experiment
After determining drug level, get cell with 1 * 10
6/ cm
2Density be inoculated in the culture bottle of 25mL, still with containing 10% hyclone, 0.3% L-glutaminate, 100UMl
-1Penicillin and 100mgL
-1Streptomycin cultivate, and establish respectively matched group and add 100,50,25mgL
-1PSP administration group cultivate, cell shines 30min under UV after cultivating 4d, spread sheet, degeneration is untwisted, electrophoresis, neutralization, dyeing, observe and film making, and on every microscope slide, select at random 10 cells to measure, and the DNA migration distance (MLD) of matched group and each administration group is such as table 2, and the MLD of each administration group compares with matched group, MLD obviously reduces, and at statistics notable difference is arranged
Table 2PSP on ultraviolet radiation 30min after the impact of MLD of cell
Repair after the damage of 3-3DNA foundation level and the damage
Give PSP again and cultivate after the foundation level damage, each time of 30,60,90min is carried out SCGE after the damage, and each organizes MLD such as the table 3 of cell and each time, Fig. 4.
After reaching, MLD when the DNA basis that table 3UV brings out is damaged gives the MLD (μ m) of each time of PSP
Annotate:
*P＜0.05,
*P＜0.01 vt matched group
Claims (6)
1. a spirulina polysaccharide organic bio-iodine is characterized in that take new living body algae mud as carrier, adds spirulina polysaccharide and organic bio-iodine, issues third contact of a total solar or lunar eclipse autotroph reaction and obtains the spirulina polysaccharide organic bio-iodine in illumination and temperature control; Described algae mud is selected from spirulina plalensis, huge spirulina or chlorella; The weight proportion of described spirulina polysaccharide organic bio-iodine is: algae mud accounts for 50-96%, and spirulina polysaccharide accounts for 3-50%, and organic bio-iodine accounts for 0.0001-10%; Described illumination is daylight or artificial light sources, its intensity of illumination 5-10 ten thousand luxs, wavelength 400-700 millimicron; Described temperature control is that temperature is between 30 ℃-40 ℃.
2. spirulina polysaccharide organic bio-iodine according to claim 1 is characterized in that described organic bio-iodine is comprised of 96% 2-Amino-3-(4-hydroxy-3-iodo-phenyl)-propionic acid, 2% 2-iodotyrosine and 2% sodium alginate.
3. spirulina polysaccharide organic bio-iodine according to claim 1 is characterized in that described spirulina polysaccharide is made through enzyme hydrolysis, precipitate with ethanol and column chromatography purification step by Spirulina powder, and wherein said enzyme is trypsin and papain.
4. spirulina polysaccharide organic bio-iodine according to claim 1 is characterized in that calculating with the dry powder of algae mud, and every 100g algae mud dry powder adds spirulina polysaccharide 3-50 gram, and organic bio-iodine calculates 100-10 with iodine, 000,000 microgram.
5. described each spirulina polysaccharide organic bio-iodine is for the preparation of the application in radiation-resistant medicine or the health food according to claim 1-4.
6. application according to claim 5, wherein radiation is ionizing radiation and examines micro-radiation or the nuclear energy radiation.
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| PCT/CN2012/073504 WO2012167656A1 (en) | 2011-06-08 | 2012-04-05 | Anti-radiation spriulina polysaccharide organic bio-active iodine and application thereof |
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| CN102851345B (en) * | 2012-08-31 | 2014-07-30 | 华南理工大学 | Preparation method for chlorella antitumor polypeptide |
| CN103013726B (en) * | 2012-12-12 | 2017-07-11 | 赵波 | The algae indigo plant green beer of polysaccharide and drinks and beverage and preparation method thereof |
| CN103923858A (en) * | 2014-04-24 | 2014-07-16 | 周霞轩 | Method for breeding iodine-rich cyanobacterium spirulina platensis iodized salt |
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| CN1173989A (en) * | 1996-12-03 | 1998-02-25 | 赵波 | Compounding process of food and drink seasoned with spirulina-active salt seasoning |
| CN1528279A (en) * | 2003-10-10 | 2004-09-15 | 波 赵 | Organic bio-iodine and its use |
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