CN103750339B - Saussurea involucrate culture health-care food and application thereof - Google Patents

Saussurea involucrate culture health-care food and application thereof Download PDF

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CN103750339B
CN103750339B CN201410008936.9A CN201410008936A CN103750339B CN 103750339 B CN103750339 B CN 103750339B CN 201410008936 A CN201410008936 A CN 201410008936A CN 103750339 B CN103750339 B CN 103750339B
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health
radix rhodiolae
saussurea
culture
care food
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CN103750339A (en
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刘汉石
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PURUIKANG BIOTECHNOLOGY CO Ltd DALIAN
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23PSHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00Shaping or working of foodstuffs characterised by the products
    • A23P10/40Shaping or working of foodstuffs characterised by the products free-flowing powder or instant powder, i.e. powder which is reconstituted rapidly when liquid is added
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The present invention provides a kind of saussurea involucrate culture health-care food and application thereof, and saussurea involucrate culture health-care food includes following weight part ratio: Saussurea involucrata culture 10~50; Radix Rhodiolae extract 50~500. Saussurea involucrata culture is a kind of novel functional food ingredient, as Medicinal Plant Cell series products, occurs in first in resisting fatigue anti-radiation health care food formula. The present invention selects Saussurea involucrata culture, Radix Rhodiolae extract to be primary raw material; through rational experimental study; it is processed into and there is alleviating physical fatigue and radiation hazradial bundle is had the health food of assistant protection function; the two is also used; can better play alleviating physical fatigue and the health care to radiation hazradial bundle; meet the deep consumer demand by the puzzlement of the two health problem; save the economic expenditure of consumer; can having more healthy health and tackle life, working and learning, therefore the present invention has wide market prospect.

Description

Saussurea involucrate culture health-care food and application thereof
Technical field
The present invention relates to a kind of saussurea involucrate culture health-care food and application thereof.
Background technology
In the face of the society that modern competition is fierce, people, in order to strive for bigger value of survival, in order to pursue higher quality of life, are required for high concentration energy all the time, to meet the challenge of competition. And pressure that the rhythm of life of consequent high speed change makes people bear is also increasing, add living habit irregular, the various reasons such as dietary structure not science, cause that the increasing people from each post stratum are subject to tired puzzlement to some extent. These cause the important background of modern society people " fatigue " symptom, " chronic fatigue syndrome " exactly, it may be said that the whole world is all in popular " fatigue ".
When its function can not be maintained at a specified level by the physiological function of body or each organ can not maintain its predetermined exercise intensity, it is called " fatigue ". Fatigue is to prevent body from the produced a kind of protective response of life-threatening excessive failure function occurring, and reminds and should reduce working strength or terminate motion when producing tired, in order to avoid body injury. Overfatigue can accelerate death, when human body is chronically under fatigue state, can produce geromorphism and fatigue syndrome. The symptom being likely to occur have not easily eliminate tired out, be weary of, there is mottle, anorexia, sexual disorder, hypertension, hyperlipidemia and fatty liver etc. in the finding it hard to concentrate of absent minded, unknown cause, dizzy, headache, constipation or diarrhoea, skin.
According to investigations: the white collars of 41.1% are faced with bigger operating pressure; In student, fatigue person accounts for 38.71% once in a while, and often fatigue person accounts for 33.87%, and fatigue person accounts for 11.29% always. In entire society, fatigue person reaches 70% more than unexpectedly, has had a strong impact on the efficiency of the study and work of people, has compromised the healthy of people, and alleviating physical fatigue is very urgent.
In the modern life, another healthy hidden danger also annoyings people deeply, that is, the harm that Radiation On Human body causes. Entering today of cyberage, electronic product act as more and more important role in life. And we are while enjoyment modern high technology offers convenience, also begin to its adverse effect to health frightened. Radiate almost all-pervasive: nearby, from the mobile phone used with oneself, to the indispensable computer of working environment, photocopier, then the TV put to family, electric refrigerator, air-conditioning, microwave oven, even people often make a move touch marble, composite floor board; At a distance, from surrounding enviroment such as periphery high-voltage line, transformer stations, to natural environment such as sunspots; Various radiation all let us are kept away and can not be kept away.
Radiation refers to a kind of state that the materials such as heat, light, sound, electromagnetic wave are propagated to surrounding, and in life, the radiation of Cucumber may bring harm to human body. The people that works, learn, live in the environment that radiation source is concentrated, it is easy to insomnia and dreamful sleep, hypomnesis, weak, the hypoimmunity of body void etc., and the speed of growth of its cancerous cell also than normal person fast 24 times.
The harm of Radiation On Human body is great: it is the main inducing of cardiovascular disease, diabetes; It can to human reproductive system, and nervous system and immune system cause direct injury; It is cause anemia of pregnant woman to miscarry, the risk factor of the pathological changes such as sterile, monster; It can directly affect child's tissue development, skeleton development, makes visual deterioration, liver hemopoietic decline; It can make Male sexual to decline, female endocrinic disorders, menoxenia etc.
China Indoor Environment Detection center also have issued electromagnetic radiation and has six warnings endangered greatly: it is most likely causes one of leukemic reason of child; It can bring out cancer and accelerate the cancer cell multiplication of human body; It affects the reproductive system of people; It may result in children's intelligence incompleteness; It affects the cardiovascular system of people; The visual system of people is had harmful effect by it. These harm ought to cause everybody attention, and actively takes measures to safeguard that our healthy is not encroached on by it.
Visible, physical fatigue and radiation hazradial bundle are slowly corroding the healthy of people, and these problems ought to be subject to everybody attention.
Thus; resisting fatigue and radioprotective have become as safeguards the healthy requisite link of people; having alleviating physical fatigue and radiation hazradial bundle has the health food of assistant protection function commercially have very big demand, the product studying and developing this function becomes particularly important.
Prior art research is as follows:
Zhang Baiqing etc. (impact on blood lactase acid after mouse movement and hepatic glycogen content of the Herba Saussureae Involueratae culture. Chinese Medicine guide, 2012,10(7): 94-95) to the Saussurea involucrata culture capsule of mouse stomach various dose continuous 30 days every day, survey its walking weight load, Serum lactic acid content and hepatic glycogen content, experimental result display Saussurea involucrata culture can extend the mice burden swimming time, reduces Serum lactic acid content, improve hepatic glycogen content, it was shown that Saussurea involucrata culture has the effect of alleviating physical fatigue.
Zhao Xiaomei etc. (research of Herba Saussureae Involueratae culture antifatigue effect. China and foreign countries' medical treatment, 2012,7:30) with Saussurea involucrata culture for object of study, tested by mice burden swimming, measure with serum urea, hepatic glycogen content measures and blood lactase acid is determined as index, and research Saussurea involucrata culture is to mouse anti-reflecting fatigue activity.Result of the test shows: Saussurea involucrata culture can extend the mice burden swimming time, reduces the increase of serum urea during mice swimming, reduces the level of serum lactic during swimming, dramatically increase liver glycogen reserves amount, have good resisting fatigue physiologically active.
Radiate the damage to body and be mainly manifested in blood system, immune infringement and inducing cell canceration aspect, particularly hemopoietic system radiation height is sensitive. Liu Yaping etc. (defencive function of radiation damage is studied by Herba Saussureae Involueratae culture. and magazine is learned in Chinese medicine basis by China; 2011; 17(9): 968-970) from blood system, chromosome and 3 aspects of oxidation-reduction system, investigate the protective effect to radiation damage of the Herba Saussureae Involueratae culture respectively. Result shows that giving Saussurea involucrata culture can significantly improve the 3rd day radiation damage model mice peripheral white blood cell after radiation; significantly improve the 14th day radiation damage model mice peripheral leukocytes after irradiation; significantly reduce radiation damage model mice bone marrow cell micronucleus rate; significantly improve superoxide dismutase activity in radiation damage model mice blood, illustrate that radiation damage is had good defencive function by Saussurea involucrata culture.
Jia Jingming etc. (radiation resistance of Herba Saussureae Involueratae tissue culture. Shenyang Pharmaceutical University's journal, 2005,22(6): 444-448,462) in order to inquire into the radiation resistance of Saussurea involucrata culture ethanol extraction, various dose Saussurea involucrata culture ethanol extraction is given to mouse stomach, every day, gavage was total to 30d 1 time, within the 20th day after gavage, was irradiated with test group 60Co γ-ray by matched group, and every day is total to 10d 1 time. Observe the change of Mice Body quality, peripheral cells number, superoxide dismutase activity, Micronucleus and mean survival time. Saussurea involucrata culture ethanol extract can make the peripheral leukocytes of Irradiated Mice, superoxide dismutase activity and mean survival time dramatically increase, Micronucleus significantly reduces in result display, describes Saussurea involucrata culture ethanol extraction and has radiation resistance.
In " the radiation resistance research of Herba Saussureae Involueratae culture " literary composition (. China and foreign countries' medical treatment, 2012,6:26), mice is divided into matched group and high, medium and low dosage group 4 groups to carry out experimentation by experimenter. Research finds to compare with matched group; give that the PCEMNR micronuclear rates that Saussurea involucrata culture can make raying reduces, mouse peripheral blood leukocyte count increases, superoxide dismutase activity increases, mean survival time increases, and illustrates that radiation damage is had defencive function by Saussurea involucrata culture.
Ancient book is early on the books to it: and Tibetan medicine's Four-Volume Medical Code record Radix Rhodiolae " cool in nature, heat clearing away, nourish vigour "; It is " draft is top grade " that " Compendium of Materia Medica-grass portion " the 20th volume indicates Radix Rhodiolae, has effects such as " strengthening the body resistance, benefiting qi and nourishing bloods ". In modern medicine study, Radix Rhodiolae is also listed in primary study object. Recent studies indicate that (the microwave extraction preliminary experiment of volatile oil in Radix Rhodiolae leaf. Guangdong pharmacy, 2002,12(6): 21-22) Radix Rhodiolae has strong, anti-hypoxia, cold resistance, resisting fatigue, radioprotective and excited brain and spinal cord effect, the work efficiency of muscle power and mental work can be improved, delay body aging.
Hao Zhaolin etc. (research of Radix Rhodiolae extract antifatigue effect. food and medicine, 2007,9(11A): 28-31) at random healthy rat is divided into 4 groups, respectively Radix Rhodiolae low dose group (0.01g/kg), middle dosage group (0.1g/kg), high dose group (0.3g/kg) and blank group (distilled water), after continuous gastric infusion 15d, carry out swimming with a load attached to the body experiment, record rat doing one's utmost property swimming time, measure blood lactase acid, serum urea nitrogen, muscle glycogen and hepatic glycogen subsequently.Test result indicate that: Radix Rhodiolae has good antifatigue effect, thereby increases and it is possible to be by increase hepatic glycogen, muscle glycogen deposit and suppress serum urea nitrogen realize.
Dai Chunmin etc. (Radix Rhodiolae research to Kunming mouse antifatigue effect. Agriculture of Anhui science, 2010,38(30): 16850-16852,16859) 40 kunming mices are selected, it is randomly divided into high, medium and low 3 test group and blank group, gavage concentration respectively 1.0g(crude drug) Radix Rhodiolae decoct 10.0g/ (kg.bw) of/ml, 5.0g/(kg.bw) and 2.5g/(kg.bw), blank group gavage equivalent distilled water, continuous gavage 3 weeks. The mice pole-climbing time is measured after 10d; Each group of mice swimming time, hepatic glycogen, muscle glycogen content is measured respectively after 3 weeks. Result records the pole-climbing time of high, medium and low dosage group, walking weight load and hepatic glycogen content and compares with blank group and all have pole significant difference, and Er Qiegao, middle dosage group hepatic glycogen content compare with low dose group and have significant difference; The muscle glycogen of gavage high dose group compares with matched group and has pole significant difference, and the muscle glycogen of middle dosage group compares with matched group and has significant difference, illustrates that Radix Rhodiolae has the effect of alleviating physical fatigue.
Yang Xiaoyan etc. (Tibetan medicine Rhodiola rosea capsules antifatigue effect research [J]. Natural products research is developed, 2009,21:480-482) in the experiment of research Tibetan medicine Rhodiola rosea capsules antifatigue effect, determine breakdown test and biochemical indicator respectively, result display experimental mice relatively control group mice walking weight load dramatically increases, after experimental mice motion, serum urea nitrogen and Serum lactic acid content significantly reduce than control group mice under the same terms, and the content of hepatic glycogen substantially increases than control group mice under the same terms. Thus showing, Rhodiola rosea capsules has obvious antifatigue effect.
Miao Yanbo etc. (radiation resistance [J] of Radix Rhodiolae. Pharmacology and Clinics of Chinese Materia Medica, 2004,20(3): 21-22) generation of the survival rate and survival mice thymus, spleen index and the heart, hepatic tissue lipid peroxide by observing mice after single-bolus high-dose (5Gy or 4Gy) deep X-ray irradiation, has carried out experimentation to the radiation resistance of Radix Rhodiolae. Research finds that the Radix Rhodiolae gastric infusion of high dose can significantly improve the survival rate accepting single-bolus high-dose deep X-ray irradiation mice, obvious increase survival mice thymus, spleen index; and dock the heart of raying mice, the generation of hepatic tissue lipid peroxide has obvious inhibitory action, it was shown that the radical damage that radiation is caused by Radix Rhodiolae has significant protective effect.
Zheng Zhi grade clearly (preliminary experiment research [J] of Radix Rhodiolae radiation-resisting functional. Shanghai preventive medicine magazine; 2000; 12(2): 69-70) carry out the preliminary experiment research of Radix Rhodiolae radiation-resisting functional, test result indicate that mice is had notable protective effect by the time-to-live after 60Co irradiation and peripheral blood leucocyte by Radix Rhodiolae ethanol extract. Prompting Radix Rhodiolae is the extremely valuable radioprotective food plant of one. Simultaneously its toxicity is little, and without obvious cumulative action, and has potential plurality of health care functions, is a kind of pole health food of having Development volue.
Liu Sanqiang (Radix Rhodiolae experimentation [J] to rat radiation damage protective action. Clinical Medical Engineering, 2010,17(2): 50-51) in order to study the Radix Rhodiolae protective action to rat radiation damage, to SD rat every day with Radix Rhodiolae extract gavage, after irradiating, measure indices with high-energy X-ray accelerator.Peripheral blood, myeloplast after rat raying are had obvious protective function by result display Radix Rhodiolae, rat chest gland and index and spleen index are had and substantially increases effect. Therefore, rat radiation damage is had obvious protective action by Radix Rhodiolae.
At present, anti-fatigue effect food mainly includes following a few class: (1), rich in vitamins functional food, is mainly vitamin A, vitamin B, vitamin C and vitamin E; (2) rich in amino acid whose functional food; (3) rich in the functional food of trace element; (4) proteins and peptides based food; (5) active polysaccharide food; (6) Chinese crude drug of resisting fatigue. Radiation-resistant food then mainly includes tea, Semen Sesami Nigrum, spirulina, Chinese herbal medicine, Thallus Laminariae (Thallus Eckloniae), Fructus Lycii, Sargassum, Radix Ginseng, Ganoderma, mushrooms, Auricularia, Amaranthus mangostanus., propolis, fruit and vegetable etc.
Summary of the invention
The person that the present invention is directed to fatiguability with contact radiation person; selecting Saussurea involucrata culture, Radix Rhodiolae extract is primary raw material; through rational experimental study; it is processed into and there is alleviating physical fatigue and radiation hazradial bundle is had the health food of assistant protection function, to reach resisting fatigue and radiation-resistant purpose simultaneously.
One of the object of the invention is to provide a kind of saussurea involucrate culture health-care food, including following weight part ratio:
Saussurea involucrata culture 10~50;
Radix Rhodiolae extract 50~500;
Saussurea involucrata culture of the present invention is the dry powder that Herba Saussureae Involueratae cultivates that the process of cell drying obtains.
Herba Saussureae Involueratae of the present invention cultivate cell be Herba Saussureae Involueratae through cell and tissue structrue gained, commercially available commercially available.
Preferably, described saussurea involucrate culture health-care food, including following weight part ratio:
Saussurea involucrata culture 10;
Radix Rhodiolae extract 60.
Preferably, described Saussurea involucrata culture is prepared by following technique:
1. lyophilization: Herba Saussureae Involueratae is cultivated cell and keeps 1~4 hour at-40~-60 DEG C; Maintain temperature at-40~-60 DEG C, be evacuated to vacuum 0.001~0.1pa, sublimation drying 5~20 hours; It is warming up to-20~-35 DEG C dry 2~6 hours, 0~-15 DEG C dry 2~6 hours, 1~20 DEG C dry 5~20 hours, 25~35 DEG C dry 3~10 hours, 40~60 DEG C dry 3~10 hours, obtains dried frozen aquatic products;
2. pulverize and sieve: after being pulverized by described dried frozen aquatic products, cross 80 mesh sieves.
The flavones content of the Saussurea involucrata culture prepared through above-mentioned technique reaches 12%, and protein content is more than 20%.
The flavones content of Saussurea involucrata culture of the present invention refers to the mass percent of flavone in Saussurea involucrata culture, and protein content refers to the mass percent of protein in Saussurea involucrata culture.
Preferably, described Radix Rhodiolae extract is prepared by following technique:
1. pulverize: Radix Rhodiolae is pulverized, obtains coarse powder;
2. extract: Radix Rhodiolae coarse powder 70% alcohol reflux 3 times, each 1h, filter, obtain ethanol extract and filtering residue;
3. concentration: ethanol extract, at temperature 60-70 DEG C, is concentrated into relative density 1.18-1.20 under vacuum 0.07-0.08Mpa, obtains concentrated solution;
4. spray drying: by concentrated solution at inlet temperature 170-180 DEG C, spray drying at leaving air temp 70-80 DEG C, obtain spray powder;
5. sieve: cross 80 mesh sieves after being pulverized by spray powder, obtain Radix Rhodiolae extract.
In the Radix Rhodiolae extract that process above prepares, Determination of Salidroside is more than 2%.
In Radix Rhodiolae extract of the present invention, Determination of Salidroside refers to the mass percent of rhodioside in Radix Rhodiolae extract.
Still a further object of the present invention is to provide a kind of saussurea involucrate culture health-care food preparation, by above-described saussurea involucrate culture health-care food, optionally adds adjuvant by the technology that well known to a person skilled in the art and is processed into preparation.
Namely need the practice with experimentation according to clinic, saussurea involucrate culture health-care food of the present invention is prepared to dosage form commonly used in the art together with adjuvant and optional coating material, such as tablet, granule, capsule, pill, syrup, powder etc.
Described adjuvant includes following optional:
Filler, selected from Icing Sugar, starch, dextrin, cyclodextrin, calcium sulfate two water thing, calcium phosphate, calcium carbonate or microcrystalline Cellulose etc.;
Binding agent, selected from starch, alginic acid and its esters, methylcellulose, sodium carboxymethyl cellulose, ethyl cellulose or polyvinylpyrrolidone etc.;
Lubricant, selected from magnesium stearate, zinc stearate, Pulvis Talci, dodecyl sodium sulfate, silicon dioxide etc.
Preferably, described saussurea involucrate culture health-care food preparation includes following weight part ratio:
Another object of the present invention is to provide described saussurea involucrate culture health-care food or the saussurea involucrate culture health-care food preparation purposes in the health food of preparation resisting fatigue radioprotective.
Herba Saussureae Involueratae is Compositae phoenix hair Dendranthema, is the perennial rare herbaceous plant in a kind of plateau, and medical value is high. Herba Saussureae Involueratae invigorating kidney, promoting blood circulation thought by Uygur medicine, bone and muscle strengthening, trophic nerve, dysregulation body fluid. The traditional Chinese medical science is thought, Herba Saussureae Involueratae bitter in the mouth warm in nature, warming the kidney to activate YANG, the effect such as have heat-clearing and toxic substances removing, wind-damp dispelling, reducing swelling and alleviating pain, restore menstrual flow and invigorate blood circulation, for antiinflammatory, dispel cold, regulating menstruation, hemostasis, enhancing immunity, slow down aging, relieving constipation, radioprotective have good action effect, modern pharmacological research proves, its effective ingredient is mainly flavone compound and polysaccharide.
Radix Rhodiolae is sweet, bitter, flat, returns lung, heart channel, has benefiting QI for activating blood circulation, effect such as relieving asthma of promoting blood circulation, it is possible to anti-hypoxia, resisting fatigue, antibacterial, analgesia, radioprotective, slow down aging etc.
Advantages of the present invention is mainly reflected in:
(1) present invention to fatiguability person with contact radiation person, select Saussurea involucrata culture, Radix Rhodiolae extract is primary raw material, through rational experimental study, it is processed into and there is alleviating physical fatigue and radiation hazradial bundle is had the health food of assistant protection function, the two is also used, can better play alleviating physical fatigue and the health care to radiation hazradial bundle, meet the deep consumer demand by the puzzlement of the two health problem, save the economic expenditure of consumer, more healthy health can be had to tackle life, working and learning, therefore the present invention has wide market prospect.
(2) Saussurea involucrata culture is a kind of novel functional food ingredient, as Medicinal Plant Cell series products, occurs in first in resisting fatigue anti-radiation health care food formula. The application of Saussurea involucrata culture had both protected wild Herba Saussureae Involueratae natural resources, turn avoid the safety problems such as pesticide, heavy metal that artificial growth Herba Saussureae Involueratae brings.
(3) Saussurea involucrata culture prepared through the present invention, general flavone content up to 12%, the material base of this radioprotective having established saussurea involucrate culture health-care food of the present invention and anti-fatigue effect.
Detailed description of the invention
Following non-limiting example can make those of ordinary skill in the art more fully understand the present invention, but does not limit the present invention in any way.
Herba Saussureae Involueratae cultivate cell be Saussurea involucrata through cell and tissue structrue gained, in aubergine, lumps, purchased from Dalian Puruikang Biotechnology Co.
Radix Rhodiolae: Radix Rhodiolae, purchased from local market, Tibet;
Radix Rhodiolae extract is prepared by following technique:
1. pulverize: Radix Rhodiolae is pulverized, obtains coarse powder;
2. extract: Radix Rhodiolae coarse powder 70% alcohol reflux 3 times, each 1h, filter, obtain ethanol extract and filtering residue;
3. concentration: ethanol extract, at temperature 60-70 DEG C, is concentrated into relative density 1.18-1.20 under vacuum 0.07-0.08Mpa, obtains concentrated solution;
4. spray drying: by concentrated solution at inlet temperature 170-180 DEG C, spray drying at leaving air temp 70-80 DEG C, obtain spray powder;
5. sieve: cross 80 mesh sieves after being pulverized by spray powder, obtain Radix Rhodiolae extract.
In the Radix Rhodiolae extract that process above prepares, Determination of Salidroside is more than 2%.
Embodiment 1
Saussurea involucrate culture health-care food, including following weight part ratio:
Saussurea involucrata culture 10; Radix Rhodiolae extract 50.
Embodiment 2
Saussurea involucrate culture health-care food, including following weight part ratio:
Saussurea involucrata culture 50; Radix Rhodiolae extract 500.
Embodiment 3
Saussurea involucrate culture health-care food, including following weight part ratio:
Saussurea involucrata culture 1; Radix Rhodiolae extract 6.
Embodiment 4
Saussurea involucrata culture 1; Radix Rhodiolae extract 6.
Embodiment 5
Saussurea involucrate culture health-care food, including following weight part ratio:
The preparation of embodiment 6 saussurea involucrate culture health-care food capsule
Raw material is prepared: Saussurea involucrata culture 1 part by following weight portion, Radix Rhodiolae extract 6 parts,
Technique: adopt the common process of capsule to produce. First by Saussurea involucrata culture fine powder and dextrin fine powder mix homogeneously, obtain mixed powder A; Again Radix Rhodiolae extract fine powder is mixed homogeneously with mixed powder A, obtain mixed powder B; Finally fine silica being mixed homogeneously with mixed powder B, the total mixed powder obtained directly fills capsule.
Test example 1
The damage of nuclear radiation on organism is mainly manifested in aspects such as blood system, immune infringement and inducing cell cancerations. In radiation damage, body also can produce substantial amounts of free radical, and medicine is by removing these free radicals, and reaches to alleviate and eliminate the damage that radiation brings. The impact of mouse peripheral blood leukocyte count and bone marrow cell micronucleus rate before and after radiation has been investigated by this research.
Experimental studies have found that, the aspect such as recovery and scavenging free radicals of the immune system of nuclear radiation injury mice, hemopoietic function is all had facilitation by Saussurea involucrata culture, Radix Rhodiolae extract, and saussurea involucrate culture health-care food of the present invention has synergism in opposing radiation, have more remarkable radioprotective effect.
Animal: Kunming kind cleaning grade female mice, is provided by Institute of Experimental Animals, Chinese Academy of Medical Sciences's breeding field.
Experimental technique:
The saussurea involucrate culture health-care food being respectively adopted embodiment 3 and embodiment 4 is given the test agent, enters as experimental group
Row test.
Respectively with Saussurea involucrata culture, Radix Rhodiolae extract for given the test agent, group is tested as a comparison, and dosage is identical with experimental group.
Kunming kind female mice, often group 10, random packet. Give 420mg/kg.BW(Mouse Weight every day) given the test agent powder, sample sterilized water configures, and once a day, per os gives, irradiation animal after continuous gavage 30d, still gives given the test agent after irradiation. Mouse stomach volume is 0.1ml/10g.BW. If radiation patterns matched group, replacing given the test agent with sterilized water, every day, gavage volume was identical with experimental group.
Leukocyte count measures: contrast groups, experimental group and radiation patterns matched group are all with same dose 60Co-gamma-rays total irradiation 1 time, and exposure dose selects 3Gy.After pre-irradiation, irradiation, within the 14th day, take blood 20 μ L, carry out leukocyte count mensuration with MEK-6318K blood analyser.
Bone marrow cell micronucleus rate: all same dose gamma total irradiation of contrast groups, experimental group and radiation patterns matched group 1 time, exposure dose selects 3Gy. After irradiating, the 3rd day cervical dislocation kills animal, microscopy, obtains bone marrow cell micronucleus rate.
On the impact (n=10) of mouse peripheral blood leukocyte count after radiation
Group and dosage Before radiation (× 109/L) 14d (× 10 after radiation9/L)
Radiation matched group 12.04±2.73 5.55±1.29
Saussurea involucrata culture contrast groups 11.93±2.50 6.44±1.29
Radix Rhodiolae extract contrast groups 11.76±2.43 6.31±1.57
Embodiment 3 experimental group 11.86±2.78 7.61±1.31
Embodiment 4 experimental group 11.98±2.36 7.96±1.66
It was found that radiation matched group leukocyte count after irradiation substantially reduces, illustrate successfully to establish Radiation damage models; And compared with matched group, the leukocyte count after the radiation of contrast groups, experimental group is all significantly improved; And embodiment 3 with the leukocyte count of embodiment 4 experimental group respectively compared with Saussurea involucrata culture contrast groups, Radix Rhodiolae extract contrast groups, leukocyte count improve more, wherein embodiment 4 experimental group is maximum.
On the impact of Micronucleus after radiation
Group and dosage Micronucleus number (individual) Bone marrow cell micronucleus rate (‰)
Radiation matched group 14.7±3.72 14.7
Saussurea involucrata culture contrast groups 13.4±2.32 13.4
Radix Rhodiolae extract contrast groups 13.1±3.32 13.1
Embodiment 3 experimental group 12.1±3.34 12.1
Embodiment 4 experimental group 11.5±2.64 11.5
It was found that compared with matched group, contrast groups, experimental group bone marrow cell micronucleus rate substantially reduce, and values of other each groups of the numeric ratio of embodiment 3 experimental group and embodiment 4 experimental group are lower, and wherein embodiment 4 experimental group is minimum.
Two above test result indicate that, saussurea involucrate culture health-care food of the present invention has collaborative resistant function for radiation hazradial bundle.
Test example 2
Evaluate tired method and have two kinds: the detection of breakdown test and biochemical indicator. Exercise tolerance be reflection organism fatigue the most directly, the most objective index. Biochemical indicator just had occurred and that change before fatigue occurs in body, therefore can reflect degree of fatigue with tired biochemical indicator and hepatic glycogen and blood lactase acid.
Blood lactase acid, as the main metabolites of musculation, is the important indicator evaluating organism fatigue. Lactic acid is piled up more many, and tired degree is more serious, and time tired, the lactic acid in muscle quickly penetrates into blood, makes Serum lactic acid content increase, until concentration reaches balance between muscle lactate and blood lactase acid. Therefore measure the Serum lactic acid content after motion stops and substantially reflecting the level of muscle lactate. Glucose is stored in liver and muscle with the form of glycogen, is called muscle glycogen and hepatic glycogen. The main source of energy when muscle glycogen is musculation, its number directly affects motor capacity. It is in the flue always simultaneous with the exhaustion of muscle glycogen that motion causes. Along with being continuously increased of muscle glycogen consumption, body, for maintaining blood sugar level, will be employed hepatic glycogen and cause that hepatic glycogen reduces. Therefore, hepatic glycogen content is the sensitive indicator of reflection degree of fatigue.
Animal: Kunming kind cleaning grade female mice, is provided by Institute of Experimental Animals, Chinese Academy of Medical Sciences's breeding field.
The saussurea involucrate culture health-care food being respectively adopted embodiment 3 and embodiment 4 is given the test agent, tests as experimental group.
Respectively with Saussurea involucrata culture, Radix Rhodiolae extract for given the test agent, group is tested as a comparison, and dosage is identical with experimental group group.
Experimental technique:
Giving 420mg/kg.BW given the test agent powder every day, sample sterilized water configures, and once a day, the Hou Jingkou that weighs gives, continuous gavage 30d.Mouse stomach volume is 0.2ml/10gBW. If matched group, replacing given the test agent with sterilized water, every day, gavage volume was identical with experimental group.
Mice burden swimming is tested: after last gives given the test agent 30min, claim weight, every rat-tail root load 5% weight sheet lead, be placed in the depth of water (30 ± 1) cm, water temperature (25 ± 1) DEG C swimming trunk went swimming, frequently stir water, make mice extremity keep motion. Record starts to death time (min) from swimming.
Hepatic glycogen measures: last is put to death mice after giving given the test agent 30min and removed liver, carries out hepatic glycogen mensuration.
Serum urea assay: after last gives given the test agent 30min, puts into not swimming with a load attached to the body 90min in the water of temperature 30 DEG C by mice, after rest 60min, plucks eyeball and takes blood, survey serum urea content.
Mice burden swimming test swimming time, serum urea and hepatic glycogen content measurement result (n=10)
Group Swimming time (min) Serum urea (mmol/L) Hepatic glycogen content (mg/100g hepatic tissue)
Matched group 32.00±9.57 6.38±0.76 6463.97±762.35
Saussurea involucrata culture contrast groups 36.21±4.56 6.01±0.73 7653.75±351.35
Radix Rhodiolae extract contrast groups 35.62±7.06 5.90±0.71 7490.19±289.36
Embodiment 3 experimental group 40.14±5.32 5.60±0.89 9096.07±304.55
Embodiment 4 experimental group 44.70±5.85 5.33±0.69 9686.03±2767.57
It was found that compared with matched group, contrast groups, experimental group swimming time are obviously prolonged, serum urea content reduces, and hepatic glycogen content raises; And, compared with Saussurea involucrata culture contrast groups, Radix Rhodiolae contrast groups, embodiment 3 experimental group and embodiment 4 experimental group more can effectively extend mice swimming time, reduce serum urea content, increasing hepatic glycogen content, i.e. resisting fatigue better effects if, wherein embodiment 4 experimental group resisting fatigue effect is best.
Thus, the Saussurea involucrata culture in saussurea involucrate culture health-care food of the present invention and Radix Rhodiolae can work in coordination with performance antifatigue effect.
Test example 3 safety testing
(1) animal acute toxicity test of saussurea involucrate culture health-care food of the present invention
It is respectively adopted Kunming mouse and each 20 of SD rat, carries out animal acute toxicity test. Test sets mono-dosage group of 15000mg/kg.BW by maximum tolerated dose method, mixes with distilled water, and 75g embodiment 3 saussurea involucrate culture health-care food adds distilled water and is settled to 200ml, gavage. Experimental result shows, MTD value is all higher than 15000mg/kg.BW, by acute toxicity powder, belongs to nontoxic level.
(2) embodiment 3 saussurea involucrate culture health-care food carrying out three genetic toxicity tests (Salmonella reversion test, mice bone marrow micronucleus and mouse inbred strain), result has no saussurea involucrate culture health-care food of the present invention mutagenic action.
Feeding trial test in (3) 30 days
SD rat, the present embodiment 3 saussurea involucrate culture health-care food sets tri-dosage groups of 350mg/kg.BW, 700mg/kg.BW, 1400mg/kg.BW, separately sets full-valence pellet feed matched group, feeding 30 days. It was found that animal growth is normal, continued weight increases, and figure is active, and defecation no abnormality seen changes; Poisoning symptom and death occur without animal; Body weight weekly, weekly food-intake, weekly food utilization and the total foodstuff utilization rate of three dosage group male and female rats, liver ratio compared with matched group, difference that there are no significant; Blood test result, latter stage Biochemistry test result compare with matched group, difference that there are no significant. Histopathological examination has no tested material high dose group and causes animal toxic injury to change.
Test example 4: the flavones content of Saussurea involucrata culture of the present invention
Flavonoid substances is resisting fatigue and radiation-resistant material base.General flavone content in this measuring embodiment of the present invention 4 Saussurea involucrata culture.
Method: Example 4 Saussurea involucrata culture is appropriate, adds methanol in right amount, is placed in apparatus,Soxhlet's, is heated to reflux. According to 2005 editions two catalogue VB of Chinese Pharmacopoeia, with rutin for reference substance, spectrophotometric determination flavones content.
Result: flavones content is up to 12% in Saussurea involucrata culture prepared by present invention process.
Test example 5: the protein content of Saussurea involucrata culture of the present invention
According to GB5009.5-2010 national food safety standard, the protein content measuring embodiment 4 Saussurea involucrata culture is 25%.
Test example 6: in Radix Rhodiolae extract of the present invention salidroside content
The method specified by " health food inspection and assessment technique specification " (version in 2003) it " in health food mensuration of rhodioloside " is tested, and measuring the content of rhodioloside in Radix Rhodiolae extract prepared by present invention process is 3%.
Test example 7: the content of rhodioloside in saussurea involucrate culture health-care food of the present invention
The method specified by " health food inspection and assessment technique specification " (version in 2003) it " in health food mensuration of rhodioloside " is tested, and measuring the content of rhodioloside in embodiment 4 saussurea involucrate culture health-care food is 1.06%. In saussurea involucrate culture health-care food of the present invention, the content of rhodioloside is the mass percent of rhodioloside in saussurea involucrate culture health-care food.

Claims (8)

1. saussurea involucrate culture health-care food, it is characterised in that include following weight part ratio:
Herba Saussureae Involueratae cultivates 10~50; Radix Rhodiolae extract 50~500;
Described Saussurea involucrata culture is prepared by following technique: 1. lyophilization: kept 1~4 hour at-40~-60 DEG C by Herba Saussureae Involueratae cell; Maintain temperature at-40~-60 DEG C, be evacuated to vacuum 0.001~0.1pa, sublimation drying 5~20 hours; It is warming up to-20~-35 DEG C dry 2~6 hours, 0~-15 DEG C dry 2~6 hours, 1~20 DEG C dry 5~20 hours, 25~35 DEG C dry 3~10 hours, 40~60 DEG C dry 3~10 hours, obtains dried frozen aquatic products; 2. pulverize and sieve: after being pulverized by described dried frozen aquatic products, cross 80 mesh sieves;
Described Radix Rhodiolae extract is prepared by following technique: 1. pulverize: is pulverized by Radix Rhodiolae, obtains coarse powder; 2. extract: Radix Rhodiolae coarse powder 70% alcohol reflux 3 times, each 1h, filter, obtain ethanol extract and filtering residue; 3. concentration: ethanol extract, at temperature 60-70 DEG C, is concentrated into relative density 1.18-1.20 under vacuum 0.07-0.08Mpa, obtains concentrated solution; 4. spray drying: by concentrated solution at inlet temperature 170-180 DEG C, spray drying at leaving air temp 70-80 DEG C, obtain spray powder; 5. sieve: cross 80 mesh sieves after being pulverized by spray powder, obtain Radix Rhodiolae extract.
2. saussurea involucrate culture health-care food according to claim 1, it is characterised in that include following weight part ratio:
Saussurea involucrata culture 10;
Radix Rhodiolae extract 60.
3. saussurea involucrate culture health-care food according to claim 1, it is characterised in that the flavones content of described Saussurea involucrata culture reaches 12%, protein content is more than 20%.
4. saussurea involucrate culture health-care food according to claim 1, it is characterised in that in described Radix Rhodiolae extract, Determination of Salidroside is more than 2%.
5. a saussurea involucrate culture health-care food preparation, by the health food described in claim 1,3,4 any one, optionally adds adjuvant by known technology and is processed into preparation.
6. saussurea involucrate culture health-care food preparation according to claim 5, it is characterised in that described preparation is soft capsule, hard capsule, tablet, tea-drinking, oral liquid, medicated wine, granule, honey ointment, distillate medicinal water or powder.
7. saussurea involucrate culture health-care food preparation according to claim 5, it is characterised in that described preparation includes following weight part ratio:
8. saussurea involucrate culture health-care food described in claim 1-4 any one or saussurea involucrate culture health-care food preparation described in the claim 5-7 any one purposes in the health food of preparation resisting fatigue radioprotective.
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