CN102309656B - Traditional Chinese medicine composition with effect of preventing and treating cancers and medicinal application of traditional Chinese medicine composition - Google Patents
Traditional Chinese medicine composition with effect of preventing and treating cancers and medicinal application of traditional Chinese medicine composition Download PDFInfo
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Abstract
The invention discloses a composition with an effect of preventing and treating cancers. The composition is mainly prepared from the following Chinese herbal medicines in percentage by mass: 20 to 90 percent of airpotato yam rhizome and 10 to 80 percent of Chinese magnoliavine fruit. The Chinese medicinal composition has the effect of preventing and treating the cancers, can eliminate the limit that liver injury is easily induced by chemotherapeutic medicinal medicines, is suitable for treating various cancers such as liver cancer, gastric cancer, breast cancer, lung cancer, bladder cancer, ovarian cancer, prostate cancer, esophagus cancer, pancreatic cancer, colon cancer, colorectal cancer, skin cancer, thyroid cancer, cervical carcinoma, leukemia and the like, can be used for preparing anti-cancer medicaments, realizes the application of the airpotato yam rhizome and Chinese magnoliavine fruit compositions to the preparation of medicaments for treating the cancers, develops the application range and the medicinal value of the airpotato yam rhizome and the Chinese magnoliavine fruit and makes creative contribution to the development of traditional Chinese medicine.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition with anti-curing cancers effect.In addition, wood invention also relates to the medical usage of this Chinese medicine composition.
Background technology
Cancer is one of principal disease of serious threat human health and life.In treatment of cancer, although traditional Radiotherapy chemotherapy makes great progress, but because it is when killing and wounding cancerous cell, also human body is produced to toxic and side effects in various degree, mainly wherein bone marrow depression and immunologic hypofunction, can cause leukopenia etc., the therapy discontinued of sometimes even having to, thus also limited the curative effect of medicine.A series of disadvantages of bringing in order to overcome traditional Radiotherapy chemotherapy, pharmacy worker constantly explores in recent years, and appliable plant medicine carries out the hot fields that anti-curing cancers becomes current research
Rhizoma Dioscoreae Bulbiferae is the dry tuber of Dioscoreaceae yam Dioscorea bulbifera L. Dioscorea bulbifera L., widely distributed in the subtropical and tropical zones in Asia, is also present in African some areas, and tradition using its as medicinal mainly in China.As far back as 500-600 in Christian era, Rhizoma Dioscoreae Bulbiferae powder, decoction or tincture, just be used to the treatment of diseases such as the goiter in modern medicine (tumor), gastric cancer, rectal cancer, laryngalgia, pharyngitis, metrorrhagia, and wherein especially conventional with the treatment of goiter (tumor) and many types of cancer; The often treatment for many types of cancers such as the esophageal carcinoma, gastric cancer, hepatocarcinoma, rectal cancer, breast carcinoma, pulmonary carcinoma, cervical cancer, nasopharyngeal carcinoma, carcinoma of maxillary sinus, bladder cancer by Rhizoma Dioscoreae Bulbiferae on modern clinic.
Compatibility is one of characteristic of middle medical drugs, can play the effect of potentiation by compatibility.By consulting after related data, inventor finds that the application of Rhizoma Dioscoreae Bulbiferae and Fructus Schisandrae Chinensis compatibility is more.Therefore, by Rhizoma Dioscoreae Bulbiferae and Fructus Schisandrae Chinensis compatibility, seeking the medicine of anti-curing cancers is safely and effectively the main starting point of the present invention.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of Chinese medicine composition with anti-curing cancers effect.This Chinese medicine composition is a kind of broad spectrum anticancer agent.
Another technical problem to be solved by this invention is to propose the application of this Chinese medicine composition in the medicine of preparation control Cancerous disease.
In order to solve the problems of the technologies described above, the Chinese medicine composition with anti-curing cancers effect that the present invention proposes, mainly the crude drug by following mass percent is prepared from: Rhizoma Dioscoreae Bulbiferae 20%～90%, Fructus Schisandrae Chinensis 10%～80%.
The preparation process that the present invention has the Chinese medicine composition of anti-curing cancers effect there is no special feature.Rhizoma Dioscoreae Bulbiferae tuber and root of Fructus Schisandrae Chinensis are beaten respectively to powder, water, 20%～95% ethanol, acetone, ethyl acetate, gasoline or petroleum ether reflux, extract,, add common medicinal adjuvant to make any pharmaceutically said dosage form, preferably extractum, fluid extract, medicated wine, tincture, oral liquid, pill, powder, drop pill, tablet or capsule.
It is closely related that Chinese medicine composition of the present invention has the synergism of anti-curing cancers effect probably and between following material: the diterpene lactone material in Rhizoma Dioscoreae Bulbiferae, especially diosbulbin A, diosbulbin B, diosbulbin C, Diosbulbin D, Diosbulbin E, Diosbulbin F, Diosbulbin G, Diosbulbin H, Diosbulbin I, Diosbulbin J, Diosbulbin K, Diosbulbin L, Diosbulbin M, and their derivant, and the lignin material in Fructus Schisandrae Chinensis, especially wherein schisandrin C, schisandrin, schisantherin B, schisantherin A, schisantherin B, schisantherin C, deoxyschizandrin and schisandrin B.
Effect experiment subsequently will prove, the alternative chemotherapeutic of Chinese medicine composition of the present invention is prevented and treated the many types of Cancerous diseases such as hepatocarcinoma, gastric cancer, breast carcinoma, pulmonary carcinoma, bladder cancer, ovarian cancer, carcinoma of prostate, the esophageal carcinoma, cancer of pancreas, colorectal cancer, colon cancer, skin carcinoma, thyroid carcinoma, cervical cancer, leukemia.
Chinese medicine composition of the present invention is the plant extract of the pure natural of dietotherapeutic, when having high-efficiency prevention and control cancer, has avoided chemotherapeutic to bring out the side effect of hepatic injury.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.These embodiment are interpreted as only for the present invention is described, is not used in and limits the scope of the invention.After having read the content of the present invention's record, those skilled in the art can make various changes or modifications the present invention, and these equivalences change and modification falls into the scope of the claims in the present invention equally.
Embodiment 1 (Rhizoma Dioscoreae Bulbiferae ethanol extract, Chinese Magnoliavine Fruit alcohol extract, the impact of Rhizoma Dioscoreae Bulbiferae Chinese Magnoliavine Fruit alcohol extract compositions on murine sarcoma S180 solid tumor)
First the present invention investigates ET and the impact of schisandra chinensis ethyl hydrate extract (SE) compositions on murine sarcoma S180 solid tumor.
1.1 experiment material
1.1.1 animal subject
ICR mice,
body weight 18～22g, purchased from Shanghai Slac Experimental Animal Co., Ltd., the animal quality certification number: SCXK (Shanghai 2007-0005).Mice raising temperature (22 ± 1) ℃, relative humidity (65 ± 10) %, lighting hours 12h every day.
1.1.2 tested medicine and preparation thereof
Rhizoma Dioscoreae Bulbiferae ethanol extract (ET): adopt Rhizoma Dioscoreae Bulbiferae tuber decoction pieces coarse powder, with 10 times of amount 80% ethanol extractions three times, each 3h, is evaporated to without alcohol taste after filtrate merging, in Rhizoma Dioscoreae Bulbiferae crude drug concentration 2g/mL, obtains Rhizoma Dioscoreae Bulbiferae ethanol extract ET.
Chinese Magnoliavine Fruit alcohol extract (SE): adopt Fructus Schisandrae Chinensis coarse powder, with 10 times of amount 70% ethanol extractions three times, each 3h, is evaporated to without alcohol taste after filtrate merging, in Fructus Schisandrae Chinensis crude drug concentration 1g/mL, obtains Chinese Magnoliavine Fruit alcohol extract SE.
Rhizoma Dioscoreae Bulbiferae Chinese Magnoliavine Fruit alcohol extract compositions: aforementioned Rhizoma Dioscoreae Bulbiferae ethanol extract and Chinese Magnoliavine Fruit alcohol extract, in 1/1～4/1 ratio (weight ratio) combination, are obtained to Rhizoma Dioscoreae Bulbiferae Chinese Magnoliavine Fruit alcohol extract compositions ET+SE.
1.2 experimental technique
Adopt experiment in tumor-bearing mice body, in sarcoma S180 tumor-bearing mice abdomen, extract corresponding tumor cell, with Trypan Blue, counting, is diluted to (1.0-1.3) * 10 with physiological saline solution
7cells/mL, in centrifuge tube, inserts on ice, and subcutaneous injection (sc) is inoculated this concentration tumor cell in the right oxter of ICR mice, and injection volume 0.1mL/ only.By body weight random packet, be respectively Control (CMC-Na), 5-Fu, Chinese Magnoliavine Fruit alcohol extract SE, Rhizoma Dioscoreae Bulbiferae ethanol extract ET, ET+SE group.Each extract or monomeric compound are dissolved to desired concn with 0.5%CMC-Na, after inoculation, 24h starts by body weight gavage (ig) administration, CMC-Na group ig gives the 0.5%CMC-Na of equal volume, 5-Fu (using dd water dissolution to 2.5mg/mL) group lumbar injection (ip), every other day injection is once; Successive administration 12d, 24h after last administration, plucks eyeball blood sampling, for measuring Serum ALT and AST vigor after centrifugalize serum; Get tumor tissue, weigh, take pictures.Press formula and calculate tumor control rate (Tumor inhibition ratio).
Tumor inhibition ratio (%)=[(C-T)/C] * 100, wherein C refers to the average tumor weight of CMC-Na matched group, T shows medicine group average tumor weight.With tumor, heavily have significant difference, and tumor control rate > 30% has the standard of anti-tumor activity for medicine.
1.3 experimental result
1.3.1ET, SE and the impact of compositions ET+SE on murine sarcoma S180 solid tumor thereof
With model group comparison, as shown in table 1, the mice of inoculation sarcoma S180 is after giving continuously ET (100mg/kg) 12d, and tumor representation work reduces (P < 0.05), shows that ET (100mg/kg) is effective to S180 solid tumor; Compare with ET (100mg/kg), after compatibility SE (100mg/kg), tumor weight average significantly reduces (P < 0.05), and tumor control rate obviously improves, and shows that Chinese Magnoliavine Fruit alcohol extract can strengthen the anti-tumor activity of Rhizoma Dioscoreae Bulbiferae ethanol extract.
Table 1ET, SE and compositions ET+SE thereof are on the impact of murine sarcoma S180 solid tumor (n=10)
With model group comparison,
*p < 0.05,
*p < 0.01; With ET comparison,
##p < 0.01
1.3.2 the impact on Biochemical Indices In Serum
As shown in Table 2, positive drug 5-Fu can make all extremely significantly risings (P < 0.01) of S180 tumor-bearing mice serum liver damage criterion ALT and AST, show that 5-Fu has induced the liver toxicity of tumor-bearing mice, and Chinese medicine composition of the present invention is when keeping curative effect, hepatic injury index is had no significant effect, illustrate that wooden invention Chinese medicine composition has the advantage of avoiding chemotherapeutic 5-Fu induction hepatic injury side effect.
Impact (the n=10 of table 2 compositions ET+SE on murine sarcoma S180 Biochemical Indices In Serum;
)
With model group comparison,
*p < 0.05,
*p < 0.01
Embodiment 2 (the full powder suspension of compositions, water extraction liquid, 70% ethanol extract, acetic acid ethyl acetate extract, acetone extract, 120# gasoline extraction thing, simulated gastric fluid extract anti-tumor in vivo effect research)
After having determined that Rhizoma Dioscoreae Bulbiferae schisandra chinensis ethyl hydrate extract compositions has stronger anti-S180 solid tumor effect, the present invention has further investigated the anti-tumor in vivo effect of suspension and the multiple extracting solution thereof of Chinese medicine composition of the present invention.
2.1 experiment material
2.1.1 animal subject (with the 1.1.1 of embodiment 1)
2.1.2 tested medicine and preparation thereof
The full powder suspension of compositions: Rhizoma Dioscoreae Bulbiferae 36g, Fructus Schisandrae Chinensis 36g, pulverizes, and crosses 150 mesh sieves, is suspended in 180mL distilled water.
Composition water extract: Rhizoma Dioscoreae Bulbiferae 54g, Fructus Schisandrae Chinensis 18g, adds 10 times of water gagings, reflux, extract, 3 times, each 3h, filters merging filtrate, and recycle-water makes into concentration 0.4g/mL.
Compositions 70% ethanol extract: Rhizoma Dioscoreae Bulbiferae 54g, Fructus Schisandrae Chinensis 18g, adds 10 times of amount 70% ethanol, reflux, extract, 3 times, each 3h, filters merging filtrate, reclaims ethanol and makes into concentration 0.4g/mL.
Compositions acetic acid ethyl acetate extract: Rhizoma Dioscoreae Bulbiferae 54g, Fructus Schisandrae Chinensis 18g, adds 10 times of amount ethyl acetate, reflux, extract, 3 times, each 3h, filters merging filtrate, reclaims ethyl acetate and makes into concentration 0.4g/mL.
Compositions acetone extract: Rhizoma Dioscoreae Bulbiferae 54g, Fructus Schisandrae Chinensis 18g, adds 10 times of amount ethyl acetate, reflux, extract, 3 times, each 3h, filters merging filtrate, reclaims acetone and makes into concentration 0.4g/mL.
Compositions 120# gasoline extraction liquid: Rhizoma Dioscoreae Bulbiferae 54g, Fructus Schisandrae Chinensis 18g, adds 10 times of amount 70% ethanol, reflux, extract, 3 times, each 3h, filters merging filtrate, reclaims 120# gasoline and makes into concentration 0.4g/mL.
The artificial gastro-intestinal Fluid extract of compositions: Rhizoma Dioscoreae Bulbiferae 54g, Fructus Schisandrae Chinensis 18g, adds 10 times of amount simulated gastric fluids, at shaking table (simulation gastrointestinal peristalsis), shake 4h, filtrate and medicinal residues are put into the simulated intestinal fluid of 10 times of amounts, on shaking table, shake 4h, collect intestinal juice, reclaim and make it concentration 0.4g/mL.
2.2 experimental techniques (with embodiment 1 1.2)
2.3 experimental result
As shown in table 3, with model group comparison, each administration group tumor weight average significantly reduces, and shows that the full powder suspension of Rhizoma Dioscoreae Bulbiferae Fructus Schisandrae Chinensis compositions and each extract all have antitumor action, wherein the strongest with the effect of 70% ethanol extract and acetic acid ethyl acetate extract; And said composition 70% ethanol and acetic acid ethyl acetate extract antitumor action are all obviously better than positive drug 5-Fu's, and compositions acetone extract, full powder suspension, water extraction liquid, acetone and gasoline extraction liquid antitumor action are suitable with 5-Fu.
Table 3 compositions suspension and multiple extracting solution are on the impact of sarcoma S180 solid tumor (n=10)
With model group comparison,
*p < 0.05,
*p < 0.01
Embodiment 3 (Chinese medicine composition and the research of active component Anticancer Activity in vitro thereof)
By Integral animal experiment, find that Chinese medicine composition of the present invention has antitumor action, inventor's separation from 70% ethanol extraction of Rhizoma Dioscoreae Bulbiferae obtains Diterpenes constituents diosbulbin B (Kawasaki T, Komori T, Setoguchi S.Furanoidnorditerpenes from Dioscoreacae plants.1.Diosbulins A, B, and C from Dioscorea bulbifera form a spontanea.Chem Pharm Bull, 1968; 16:2430-2435.) (purity is more than 98%); Lignin compound schisandrin C, schisandrin, schisantherin B, schisantherin A, schisantherin B, schisantherin C, deoxyschizandrin and schisandrin B contained in Fructus Schisandrae Chinensis are all purchased from Shanghai warm medical sci-tech Development Co., Ltd, and purity is all more than 95%.Inventor has further investigated the active anticancer of this invention Chinese medicine composition and active component thereof by experiment in vitro.
By take in Fructus Schisandrae Chinensis lignin composition, the strongest schisandrin C of Rhizoma Dioscoreae Bulbiferae active component diosbulbin B potentiation is introduced as example below.Wherein, the chemical constitution of diosbulbin B (Diosbulbin B) and schisandrin C (Schizandrin C) is as follows.
3.1 experiment material
3.1.1 cell strain is originated
Hep3B cells, gastric cancer SGC-7901, cancer of pancreas BXPC-3, breast carcinoma MCF-7, pulmonary carcinoma A549, bladder cancer T24 human ovarian cancer HO8910, colon cancer LS174, colorectal cancer CCL229, carcinoma of prostate PC-3, hunman esophageal carcinoma Eca-109 cells, skin carcinoma A431, thyroid carcinoma SW-579, leukemia K 562, s strain are all purchased from Shanghai Inst. of Cytobiology, Chinese Academy of Sciences.
3.1.2 main agents
IV Collagenase Type (Sigma company), DEME culture medium (GIBCO company), hyclone (Hangzhou Ilex purpurea Hassk.[I.chinensis Sims biotech firm), dimethyl sulfoxide (DMSO) (GIBCO company), MTT (Sigma company), protease inhibitor (Amresco), 5-Fu (Shanghai nation one-tenth), other reagent is domestic analytical pure etc.
3.1.3 key instrument equipment
Laminar flow super-clean bench; CO2 gas incubator, purchased from Thermo company; Inverted phase contrast microscope, purchased from Olympus company; Microplate reader, purchased from Biotek company.
3.2 experimental technique
Cell proliferation experiment (MTT): use 0.25% trypsin digestion cell, 1000rpm is centrifugal, with containing 5% active carbon inactivated serum, without 37 ℃ of the high sugared culture fluid of phenol red DMEM, 95%CO
2cultivate 48h (to get rid of the interference of endogenous hormones), cell enters exponential phase, uses 0.25% trypsin digestion cell, with 2 * 10
3individual/hole inoculating cell on 96 well culture plates, every pore volume 180 μ L, 24h cell attachment, every row are established 5 multiple holes, establish respectively solvent control, 5-Fu positive control, Experimental agents group, continue to observe to cultivate 48h after dosing.
Colour generation and colorimetric: in dosing, cultivate after 48h, take out culture plate, every hole adds MTT solution (5mg/mL) 20 μ L, 37 ℃, 5%CO
2continuing to cultivate 4h stops cultivating.Careful suction abandoned culture supernatant in hole, every hole adds 150 μ L DMSO vibration 10min, and crystal is fully dissolved, and selects 490nm at upper each hole absorption value (OD of mensuration of automatic microplate reader (BIO-TEK), be directly proportional to living cells quantity), calculate inhibitory rate of cell growth (IR).IR=(control wells OD value-experimental port OD value)/control wells OD value * 100%.
3.3 experimental result
From table 4-8, Chinese medicine composition 70% ethanol extract of the present invention and diterpenoid-lactone active component diosbulbin B thereof all can significantly suppress human liver cancer cell Hep3B, stomach cancer cell SGC-7901, transitional cell bladder carcinoma cell line T24, ovarian cancer cell HO8910, pancreatic cancer cell BXPC-3, breast cancer cell MCF-7, lung cell A549, colon cancer cell LS174, colorectal cancer cells CCL229, prostate gland cancer cell PC-3, esophageal cancer cell Eca-109, skin cancer cell A431, thyroid carcinoma cell SW-579, leukaemia K562, the growth of cervical cancer cell Hela, point out this Chinese medicine composition and Diterpenes constituents diosbulbin B thereof to can be used for Hepatoma therapy, gastric cancer, breast carcinoma, pulmonary carcinoma, bladder cancer, ovarian cancer, carcinoma of prostate, the esophageal carcinoma, cancer of pancreas, colorectal cancer, colon cancer, skin carcinoma, thyroid carcinoma, cervical cancer, the treatment of the many types of cancer such as leukemia.Simultaneously, the present invention has also investigated the anticancer effect of active ingredient compositions, found that in the contained lignin composition of Fructus Schisandrae Chinensis such as schisandrin C, when with diosbulbin B compatibility, can significantly strengthen the antitumaous effect of diosbulbin B to above multiple cancerous cell, and the active anticancer after compatibility is better than chemotherapeutic 5-Fu's, the contained lignin composition of prompting Fructus Schisandrae Chinensis can be with contained Diterpenes constituents compatibility efficient application in Rhizoma Dioscoreae Bulbiferae in the above Cancerous disease for the treatment of, and can be used as the selection that substitutes chemotherapeutic 5-Fu application.
The impact on people's cancer BXPC-3, MCF-7, A549 inhibitory rate of cell growth (%) of table 4 compositions and active component thereof
With the comparison of solvent control group,
*p < 0.01; With 5-Fu comparison,
#p < 0.05
The impact on people's cancer SGC-7901, Hep3B, T24 inhibitory rate of cell growth (%) of table 5 compositions and active component thereof
With the comparison of solvent control group,
*p < 0.01; With 5-Fu comparison,
#p < 0.05
The impact on people's cancer HO8910, LS174, PC-3 inhibitory rate of cell growth (%) of table 6 compositions and active component thereof
With the comparison of solvent control group,
*p < 0.01 and 5-Fu comparison,
#p < 0.05
The impact on people's cancer CCL229, Eca-109, A431 inhibitory rate of cell growth (%) of table 7 compositions and active component thereof
With the comparison of solvent control group,
*p < 0.01; With 5-Fu comparison,
#p < 0.05
The impact on people's cancer SW-579, K562, Hela inhibitory rate of cell growth (%) of table 8 compositions and active component thereof
With the comparison of solvent control group,
*p < 0.01; With 5-Fu comparison,
#p < 0.05
Embodiment 4 (preparation of fluid extract)
Get Rhizoma Dioscoreae Bulbiferae 600g, Fructus Schisandrae Chinensis 200g, beat powder, with 70% ethanol, make solvent, dipping 48h, slowly percolation, collects the liquid 425mL that just filters, and another device is preserved; Continue percolation, colourless to percolate approaches, collect the continuous liquid of filtering, at 55 ℃, be concentrated into thick paste shape, add the first liquid 680mL that filters to mix, with 70% ethanol dilution to 800mL, standing 3d, filtration, obtains.
Embodiment 5 (preparation of tablet)
Get Rhizoma Dioscoreae Bulbiferae 600g, Fructus Schisandrae Chinensis 200g, beat powder, cross 100 mesh sieves.Spray into appropriate 70% ethanol, add 17.5% microcrystalline Cellulose, add 7.5% microcrystalline Cellulose and 20% starch in wherein, granulation, dry, additional 10% microcrystalline Cellulose and 1% magnesium stearate, mix tabletting.
Embodiment 6 (preparation of drop pill)
Get Rhizoma Dioscoreae Bulbiferae 600g, Fructus Schisandrae Chinensis 200g, beat powder, with 70% alcohol reflux 3 times, each 2h, reclaims ethanol and obtains extractum, take PGE4000 as substrate, with the ratio of medicine be 1: 1.5, dimethicone is condensing agent, dripping.
Embodiment 7 (preparation of capsule)
Get Rhizoma Dioscoreae Bulbiferae 600g, Fructus Schisandrae Chinensis 200g, beat powder, by ethyl acetate backflow, extract 3 times, each 2h, reclaims ethyl acetate and obtains extractum, dry, pulverize, and crosses 100 mesh sieves, adds 5% magnesium stearate, mixes and packs hard capsule into.
Claims (4)
1. a Chinese medicine composition with anti-curing cancers effect, is characterized in that, by Rhizoma Dioscoreae Bulbiferae ethanol extraction and schisandra chinensis ethyl hydrate extract, the weight ratio with 100:150 mixes for it, wherein:
Aforementioned Rhizoma Dioscoreae Bulbiferae ethanol extraction system adopts Rhizoma Dioscoreae Bulbiferae tuber decoction pieces coarse powder, and with 10 times of amount 80% ethanol extractions three times, each 3h, is evaporated to without alcohol taste after filtrate merging, and the Rhizoma Dioscoreae Bulbiferae crude drug concentration of take is 2g/mL;
Aforementioned schisandra chinensis ethyl hydrate extract system adopts Fructus Schisandrae Chinensis coarse powder, and with 10 times of amount 70% ethanol extractions three times, each 3h, is evaporated to without alcohol taste after filtrate merging, and the Fructus Schisandrae Chinensis crude drug concentration of take is 1g/mL.
2. the Chinese medicine composition with anti-curing cancers effect according to claim 1, is characterized in that, this Chinese medicine composition is extractum, fluid extract, medicated wine, tincture, oral liquid, pill, powder, tablet or capsule.
3. the Chinese medicine composition with anti-curing cancers effect according to claim 2, is characterized in that, pill is drop pill.
4. in claim 1-3, the Chinese medicine composition described in any one is prevented and treated the application in hepatocarcinoma, gastric cancer, breast carcinoma, pulmonary carcinoma, bladder cancer, ovarian cancer, carcinoma of prostate, the esophageal carcinoma, cancer of pancreas, colorectal cancer, colon cancer, skin carcinoma, thyroid carcinoma, cervical cancer or leukemic medicine in preparation.
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| CN108836963B (en) * | 2018-05-18 | 2020-06-05 | 山东省中医药研究院 | Application of diosbulbin B in preparation of drug for treating nodular goiter |
| CN114081873A (en) * | 2021-10-19 | 2022-02-25 | 广州医科大学附属第二医院 | Application of deoxyschizandrin |
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| C06 | Publication | ||
| PB01 | Publication | ||
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