CN102309656A - Chinese medicinal composition with effect of preventing and treating cancers and medicinal application of Chinese medicinal composition - Google Patents
Chinese medicinal composition with effect of preventing and treating cancers and medicinal application of Chinese medicinal composition Download PDFInfo
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Abstract
The invention discloses a composition with an effect of preventing and treating cancers. The composition is mainly prepared from the following Chinese herbal medicines in percentage by mass: 20 to 90 percent of airpotato yam rhizome and 10 to 80 percent of Chinese magnoliavine fruit. The Chinese medicinal composition has the effect of preventing and treating the cancers, can eliminate the limit that liver injury is easily induced by chemotherapeutic medicinal medicines, is suitable for treating various cancers such as liver cancer, gastric cancer, breast cancer, lung cancer, bladder cancer, ovarian cancer, prostate cancer, esophagus cancer, pancreatic cancer, colon cancer, colorectal cancer, skin cancer, thyroid cancer, cervical carcinoma, leukemia and the like, can be used for preparing anti-cancer medicaments, realizes the application of the airpotato yam rhizome and Chinese magnoliavine fruit compositions to the preparation of medicaments for treating the cancers, develops the application range and the medicinal value of the airpotato yam rhizome and the Chinese magnoliavine fruit and makes creative contribution to the development of traditional Chinese medicine.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition with anti-curing cancers effect.In addition, the wood invention also relates to the medical usage of this Chinese medicine composition.
Background technology
Cancer is one of principal disease of serious threat human health and life.In treatment of cancer; Although traditional put, chemotherapy obtained very big progress, because it when killing and wounding cancerous cell, also produces toxic and side effects in various degree to human body; Wherein mainly be bone marrow depression and immunologic hypofunction; Can cause leukopenia etc., sometimes even the TD of having to, thereby also limit the curative effect of medicine.A series of disadvantages of traditional put in order to overcome, chemotherapy being brought, the pharmacy worker constantly explores in recent years, and the appliable plant medicine prevents that curing cancers becomes the hot fields of current research
Rhizoma Dioscoreae Bulbiferae is the dry tuber of Dioscoreaceae yam Dioscorea bulbifera L. Dioscorea bulbifera L., distributes extensively in the torrid zone in Asia and subtropical zone, also is present in the some areas in Africa, and tradition with its as medicinal mainly in China.As far back as 500-600 in Christian era; Rhizoma Dioscoreae Bulbiferae powder, decoction or tincture; Just be used to such as treatment of conditions such as the goiter in the modern medicine (tumor), gastric cancer, rectal cancer, laryngalgia, pharyngitis, metrorrhagia, and wherein commonly used with goiter (tumor) and many types of treatment for cancer especially; Modern times often are used for Rhizoma Dioscoreae Bulbiferae many types of treatment for cancer such as the esophageal carcinoma, gastric cancer, hepatocarcinoma, rectal cancer, breast carcinoma, pulmonary carcinoma, cervical cancer, nasopharyngeal carcinoma, carcinoma of maxillary sinus, bladder cancer clinically.
Compatibility is one of characteristic of middle medical drugs, can play synergic effect through compatibility.After consulting related data, the inventor finds that Rhizoma Dioscoreae Bulbiferae and Fructus Schisandrae Chinensis compatibility use more.Therefore, seeking safely and effectively through Rhizoma Dioscoreae Bulbiferae and Fructus Schisandrae Chinensis compatibility, the medicine of anti-curing cancers is the main starting point of the present invention.
Summary of the invention
Technical problem to be solved by this invention provides a kind of Chinese medicine composition with anti-curing cancers effect.This Chinese medicine composition is a kind of broad spectrum anticancer agent.
Another technical problem to be solved by this invention is to propose the application of this Chinese medicine composition in the medicine of preparation control Cancerous disease.
In order to solve the problems of the technologies described above, the Chinese medicine composition with anti-curing cancers effect that the present invention proposes mainly is prepared from the following raw materials by weight percentage medicine: Rhizoma Dioscoreae Bulbiferae 20%～90%, Fructus Schisandrae Chinensis 10%～80%.
The preparation process that the present invention has the Chinese medicine composition of anti-curing cancers effect does not have special feature.Rhizoma Dioscoreae Bulbiferae tuber and root of Fructus Schisandrae Chinensis are beaten powder respectively; Water, 20%～95% ethanol, acetone, ethyl acetate, gasoline or petroleum ether reflux, extract; Add common pharmaceutic adjuvant and process any pharmaceutically said dosage form, preferred extractum, fluid extract, medicated wine, tincture, oral liquid, pill, powder, drop pill, tablet or capsule.
Chinese medicine composition of the present invention have anti-curing cancers effect probably and the synergism between the following material closely related: the diterpene lactone material in the Rhizoma Dioscoreae Bulbiferae; Especially diosbulbin A, diosbulbin B, diosbulbin C, Diosbulbin D, Diosbulbin E, Diosbulbin F, Diosbulbin G, Diosbulbin H, Diosbulbin I, Diosbulbin J, Diosbulbin K, Diosbulbin L, Diosbulbin M; And their derivant; And the lignin material in the Fructus Schisandrae Chinensis, schisandrin C especially wherein, schisandrin, schisantherin B, schisantherin A, schisantherin B, schisantherin C, deoxyschizandrin and schisandrin B.
Effect experiment subsequently will prove that the alternative chemotherapeutic of Chinese medicine composition of the present invention is prevented and treated many types of Cancerous diseases such as hepatocarcinoma, gastric cancer, breast carcinoma, pulmonary carcinoma, bladder cancer, ovarian cancer, carcinoma of prostate, the esophageal carcinoma, cancer of pancreas, colorectal cancer, colon cancer, skin carcinoma, thyroid carcinoma, cervical cancer, leukemia.
Chinese medicine composition of the present invention is the pure natural plants extract of dietotherapeutic, when having the high-efficiency prevention and control cancer, has avoided chemotherapeutic to bring out the side effect of hepatic injury.
The specific embodiment
Further set forth the present invention below in conjunction with specific embodiment.These embodiment are interpreted as only being used to the present invention is described and are not used in restriction protection scope of the present invention.After the content of having read the present invention's record, those skilled in the art can do various changes or modification to the present invention, and these equivalences change and modify and fall into claim of the present invention institute restricted portion equally.
Embodiment 1 (Rhizoma Dioscoreae Bulbiferae ethanol extract, Chinese Magnoliavine Fruit alcohol extract, Rhizoma Dioscoreae Bulbiferae Chinese Magnoliavine Fruit alcohol extract compositions are to the influence of murine sarcoma S180 solid tumor)
The present invention at first investigates the influence to murine sarcoma S180 solid tumor of ET and schisandra chinensis ethyl hydrate extract (SE) compositions.
1.1 experiment material
1.1.1 animal subject
The ICR mice;
body weight 18～22g; Available from Shanghai Slac Experimental Animal Co., Ltd., the animal quality certification number: SCXK (Shanghai 2007-0005).Mice raising temperature (22 ± 1) ℃, relative humidity (65 ± 10) %, lighting hours 12h every day.
1.1.2 receive reagent thing and preparation thereof
Rhizoma Dioscoreae Bulbiferae ethanol extract (ET): adopt Rhizoma Dioscoreae Bulbiferae tuber decoction pieces coarse powder, with 10 times of amount 80% ethanol extractions three times, each 3h, being evaporated to after filtrating merges does not have alcohol and distinguishes the flavor of, and in Rhizoma Dioscoreae Bulbiferae crude drug concentration 2g/mL, promptly gets Rhizoma Dioscoreae Bulbiferae ethanol extract ET.
Chinese Magnoliavine Fruit alcohol extract (SE): adopt the Fructus Schisandrae Chinensis coarse powder, with 10 times of amount 70% ethanol extractions three times, each 3h, being evaporated to after filtrating merges does not have alcohol and distinguishes the flavor of, and in Fructus Schisandrae Chinensis crude drug concentration 1g/mL, promptly gets Chinese Magnoliavine Fruit alcohol extract SE.
Rhizoma Dioscoreae Bulbiferae Chinese Magnoliavine Fruit alcohol extract compositions: aforementioned Rhizoma Dioscoreae Bulbiferae ethanol extract and Chinese Magnoliavine Fruit alcohol extract in 1/1～4/1 ratio (weight ratio) combination, are promptly got Rhizoma Dioscoreae Bulbiferae Chinese Magnoliavine Fruit alcohol extract compositions ET+SE.
1.2 experimental technique
Adopt experiment in the tumor-bearing mice body, in sarcoma S180 tumor-bearing mice abdomen, extract corresponding tumor cell, with trypan blue dyeing, counting is diluted to (1.0-1.3) * 10 with physiological saline solution
7Cells/mL inserts on ice in centrifuge tube, and subcutaneous injection (sc) is inoculated this concentration tumor cell in the right oxter of ICR mice, and injection volume 0.1mL/ only.By the body weight random packet, be respectively Control (CMC-Na), 5-Fu, Chinese Magnoliavine Fruit alcohol extract SE, Rhizoma Dioscoreae Bulbiferae ethanol extract ET, ET+SE group.Each extract or monomeric compound are dissolved to desired concn with 0.5%CMC-Na; 24h begins to irritate stomach (ig) administration by body weight in the inoculation back; CMC-Na group ig gives the 0.5%CMC-Na of equal volume, and 5-Fu (with the dd water dissolution to 2.5mg/mL) group lumbar injection (ip) is every other day injected once; Successive administration 12d, 24h after the last administration plucks the eyeball blood sampling, supplies to measure Serum ALT and AST vigor usefulness behind the centrifugalize serum; Get tumor tissue, weigh, take pictures.Press formula and calculate tumor control rate (Tumor inhibition ratio).
Tumor inhibition ratio (%)=[(C-T)/C] * 100, wherein C refers to that the average tumor of CMC-Na matched group is heavy, it is heavy that T shows the average tumor of medicine group.With tumor significant difference is arranged heavily, and tumor control rate＞30% has the standard of anti-tumor activity for medicine.
1.3 experimental result
1.3.1ET, SE and compositions ET+SE thereof be to the influence of murine sarcoma S180 solid tumor
Compare with model group, as shown in table 1, the mice of inoculation sarcoma S180 is after giving ET (100mg/kg) 12d continuously, and tumor representation work reduces (P＜0.05), shows that ET (100mg/kg) is effective to the S180 solid tumor; Compare with ET (100mg/kg), behind the compatibility SE (100mg/kg), the tumor weight average significantly reduces (P＜0.05), and tumor control rate obviously improves, and shows that Chinese Magnoliavine Fruit alcohol extract can strengthen the anti-tumor activity of Rhizoma Dioscoreae Bulbiferae ethanol extract.
Table 1ET, SE and compositions ET+SE thereof are to the influence (n=10) of murine sarcoma S180 solid tumor
Compare with model group,
*P＜0.05,
*P＜0.01; Compare with ET,
##P＜0.01
1.3.2 influence to the serum biochemistry index
Can know by table 2; Positive drug 5-Fu can make all extremely significantly risings (P＜0.01) of S180 tumor-bearing mice serum liver damage criterion ALT and AST; Show that 5-Fu has induced the liver toxicity of tumor-bearing mice; And Chinese medicine composition of the present invention does not have obvious influence to the hepatic injury index when keeping curative effect, explain that wood invents Chinese medicine composition and have and avoid chemotherapeutic 5-Fu to induce the advantage of hepatic injury side effect.
Table 2 compositions ET+SE is to the influence (n=10 of murine sarcoma S180 serum biochemistry index;
)
Compare with model group,
*P＜0.05,
*P＜0.01
Embodiment 2 (the full powder suspension of compositions, water extract, 70% ethanol extract, acetic acid ethyl acetate extract, acetone extract, 120# gasoline extraction thing, simulated gastric fluid extract anti-tumor in vivo effect research)
After having confirmed that Rhizoma Dioscoreae Bulbiferae schisandra chinensis ethyl hydrate extract compositions has stronger anti-S180 solid tumor effect, the present invention has further investigated the anti-tumor in vivo effect of the suspension and the multiple extracting solution thereof of Chinese medicine composition of the present invention.
2.1 experiment material
2.1.1 animal subject (with the 1.1.1 of embodiment 1)
2.1.2 receive reagent thing and preparation thereof
The full powder suspension of compositions: Rhizoma Dioscoreae Bulbiferae 36g, Fructus Schisandrae Chinensis 36g pulverizes, and crosses 150 mesh sieves, is suspended in the 180mL distilled water.
Composition water extract: Rhizoma Dioscoreae Bulbiferae 54g, Fructus Schisandrae Chinensis 18g adds 10 times of water gagings, reflux, extract, 3 times, each 3h filters merging filtrate, and recycle-water makes into concentration 0.4g/mL.
Compositions 70% ethanol extract: Rhizoma Dioscoreae Bulbiferae 54g, Fructus Schisandrae Chinensis 18g adds 10 times of amount 70% ethanol, reflux, extract, 3 times, each 3h filters merging filtrate, reclaims ethanol and makes into concentration 0.4g/mL.
The compositions acetic acid ethyl acetate extract: Rhizoma Dioscoreae Bulbiferae 54g, Fructus Schisandrae Chinensis 18g adds 10 times of amount ethyl acetate, reflux, extract, 3 times, each 3h filters merging filtrate, reclaims ethyl acetate and makes into concentration 0.4g/mL.
The compositions acetone extract: Rhizoma Dioscoreae Bulbiferae 54g, Fructus Schisandrae Chinensis 18g adds 10 times of amount ethyl acetate, reflux, extract, 3 times, each 3h filters merging filtrate, reclaims acetone and makes into concentration 0.4g/mL.
Compositions 120# gasoline extraction liquid: Rhizoma Dioscoreae Bulbiferae 54g, Fructus Schisandrae Chinensis 18g adds 10 times of amount 70% ethanol, reflux, extract, 3 times, each 3h filters merging filtrate, reclaims 120# gasoline and makes into concentration 0.4g/mL.
The artificial gastro-intestinal Fluid extract of compositions: Rhizoma Dioscoreae Bulbiferae 54g, Fructus Schisandrae Chinensis 18g adds 10 times of amount simulated gastric fluids; Shake 4h at shaking table (simulated gastric enterokinesia), filtrating and medicinal residues are put into the simulated intestinal fluid of 10 times of amounts, on shaking table, shake 4h; Collect intestinal juice, reclaim and make it concentration 0.4g/mL.
2.2 experimental technique (with embodiment 1 1.2)
2.3 experimental result
As shown in table 3, compare with model group, each administration group tumor weight average significantly reduces, and shows that the full powder suspension of Rhizoma Dioscoreae Bulbiferae Fructus Schisandrae Chinensis compositions and each extract all have antitumor action, and is wherein the strongest with the effect of 70% ethanol extract and acetic acid ethyl acetate extract; And said composition 70% ethanol and acetic acid ethyl acetate extract antitumor action all obviously are better than positive drug 5-Fu's, and compositions acetone extract, full powder suspension, water extract, acetone and gasoline extraction liquid antitumor action are suitable with 5-Fu.
Table 3 compositions suspension and multiple extracting solution are to the influence (n=10) of sarcoma S180 solid tumor
Compare with model group,
*P＜0.05,
*P＜0.01
Embodiment 3 (the external Anticancer Activities of Chinese medicine composition and active component thereof)
Find that through the whole animal experiment Chinese medicine composition of the present invention has antitumor action; The inventor separates from 70% ethanol extraction of Rhizoma Dioscoreae Bulbiferae and obtains diterpenoid-lactone constituents diosbulbin B (Kawasaki T; Komori T, Setoguchi S.Furanoidnorditerpenes from Dioscoreacae plants.1.Diosbulins A, B; And C from Dioscorea bulbifera form a spontanea.Chem Pharm Bull, 1968; 16:2430-2435.) (purity is more than 98%); Lignin chemical compound schisandrin C, schisandrin, schisantherin B, schisantherin A, schisantherin B, schisantherin C, deoxyschizandrin and schisandrin B contained in the Fructus Schisandrae Chinensis are all available from the warm medical sci-tech in Shanghai Development Co., Ltd, and purity is all more than 95%.The inventor has further investigated the active anticancer of this invention Chinese medicine composition and active component thereof through experiment in vitro.
Below will be being that example is introduced to the strongest schisandrin C of Rhizoma Dioscoreae Bulbiferae active component diosbulbin B potentiation in the Fructus Schisandrae Chinensis lignin composition.Wherein, the chemical constitution of diosbulbin B (Diosbulbin B) and schisandrin C (Schizandrin C) is following.
3.1 experiment material
3.1.1 cell strain source
People's hepatocarcinoma Hep3B, gastric cancer SGC-7901, cancer of pancreas BXPC-3, breast carcinoma MCF-7, pulmonary carcinoma A549, bladder cancer T24 HOC HO8910, colon cancer LS174, colorectal cancer CCL229, carcinoma of prostate PC-3, esophageal carcinoma Eca-109, skin carcinoma A431, thyroid carcinoma SW-579, leukemia K 562, s strain are all available from Shanghai Inst. of Cytobiology, Chinese Academy of Sciences.
3.1.2 main agents
IV Collagen Type VI enzyme (Sigma company), DEME culture medium (GIBCO company), hyclone (Hangzhou Ilex purpurea Hassk.[I.chinensis Sims biotech firm); Dimethyl sulfoxide (DMSO) (GIBCO company), MTT (Sigma company), protease inhibitor (Amresco); 5-Fu (Shanghai nation one-tenth), other reagent is homemade analytical pure etc.
3.1.3 key instrument equipment
The laminar flow super-clean bench; CO2 gas incubator is available from Thermo company; Inverted phase contrast microscope is available from Olympus company; ELIASA is available from Biotek company.
3.2 experimental technique
Cell proliferation experiment (MTT): use 0.25% trypsin digestion cell, 1000rpm is centrifugal, with containing 5% active carbon inactivated serum, 37 ℃ of the high sugared culture fluid of no phenol red DMEM, 95%CO
2Cultivate 48h (to get rid of the interference of endogenous hormones), promptly cell gets into exponential phase, uses 0.25% trypsin digestion cell, with 2 * 10
3Individual/hole inoculating cell is on 96 well culture plates, and every pore volume 180 μ L, 24h cell attachment, every row establish 5 multiple holes, establishes solvent control, 5-Fu positive control, experiment drug group respectively, and the dosing continued is observed and cultivated 48h.
Colour generation and colorimetric: after 48h is cultivated in dosing, take out culture plate, every hole adds MTT solution (5mg/mL) 20 μ L, 37 ℃, 5%CO
2Continuing to cultivate 4h stops cultivating.The careful suction abandoned culture supernatant in the hole; Every hole adds 150 μ L DMSO vibration 10min, and crystal is fully dissolved, and selects 490nm to go up at automatic ELIASA (BIO-TEK) and measures each hole absorption value (OD; Be directly proportional with living cells quantity), calculate inhibitory rate of cell growth (IR).IR=(control wells OD value-experimental port OD value)/control wells OD value * 100%.
3.3 experimental result
4-8 can know by table; Chinese medicine composition 70% ethanol extract of the present invention and diterpenoid-lactone active component diosbulbin B thereof all can significantly suppress the growth of human liver cancer cell Hep3B, stomach cancer cell SGC-7901, transitional cell bladder carcinoma cell line T24, ovarian cancer cell HO8910, pancreatic cancer cell BXPC-3, breast cancer cell MCF-7, lung cell A549, colon cancer cell LS174, colorectal cancer cells CCL229, prostate gland cancer cell PC-3, esophageal cancer cell Eca-109, skin cancer cell A431, thyroid carcinoma cell SW-579, leukaemia K562, cervical cancer cell Hela, point out this Chinese medicine composition and diterpenoid-lactone constituents diosbulbin B thereof to can be used for treating many types of treatment for cancer such as hepatocarcinoma, gastric cancer, breast carcinoma, pulmonary carcinoma, bladder cancer, ovarian cancer, carcinoma of prostate, the esophageal carcinoma, cancer of pancreas, colorectal cancer, colon cancer, skin carcinoma, thyroid carcinoma, cervical cancer, leukemia.Simultaneously; The present invention has also investigated the anticancer effect of active ingredient compositions; The result finds in the contained lignin composition of Fructus Schisandrae Chinensis such as schisandrin C; With the diosbulbin B compatibility time, can significantly strengthen the antitumaous effect of diosbulbin B to above multiple cancerous cell; And the active anticancer behind the compatibility is better than chemotherapeutic 5-Fu's, the contained lignin composition of prompting Fructus Schisandrae Chinensis can with contained diterpene lactone components compatibility efficient application in the Rhizoma Dioscoreae Bulbiferae in the above Cancerous disease of treatment, and can be used as and substitute the selection that chemotherapeutic 5-Fu uses.
Table 4 compositions and active component thereof are to the influence of people's cancer BXPC-3, MCF-7, A549 inhibitory rate of cell growth (%)
Compare with solvent control group,
*P＜0.01; Compare with 5-Fu,
#P＜0.05
Table 5 compositions and active component thereof are to the influence of people's cancer SGC-7901, Hep3B, T24 inhibitory rate of cell growth (%)
Compare with solvent control group,
*P＜0.01; Compare with 5-Fu,
#P＜0.05
Table 6 compositions and active component thereof are to the influence of people's cancer HO8910, LS174, PC-3 inhibitory rate of cell growth (%)
Compare with solvent control group,
*Compare with 5-Fu P＜0.01,
#P＜0.05
Table 7 compositions and active component thereof are to the influence of people's cancer CCL229, Eca-109, A431 inhibitory rate of cell growth (%)
Compare with solvent control group,
*P＜0.01; Compare with 5-Fu,
#P＜0.05
Table 8 compositions and active component thereof are to the influence of people's cancer SW-579, K562, Hela inhibitory rate of cell growth (%)
Compare with solvent control group,
*P＜0.01; Compare with 5-Fu,
#P＜0.05
Embodiment 4 (preparation of fluid extract)
Get Rhizoma Dioscoreae Bulbiferae 600g, Fructus Schisandrae Chinensis 200g, beat powder, make solvent with 70% ethanol, dipping 48h, slowly percolation is collected the liquid 425mL that just filters, and device is preserved in addition; Continue percolation, near till colourless, collect the continuous liquid of filtering, be concentrated into the thick paste shape, add the liquid 680mL mixing of just filtering, to 800mL, leave standstill 3d, filter, promptly get with 70% ethanol dilution at 55 ℃ to percolate.
Embodiment 5 (preparation of tablet)
Get Rhizoma Dioscoreae Bulbiferae 600g, Fructus Schisandrae Chinensis 200g, beat powder, cross 100 mesh sieves.Spray into an amount of 70% ethanol, add 17.5% microcrystalline Cellulose, add 7.5% microcrystalline Cellulose and 20% starch in wherein, process granule, drying adds the magnesium stearate of 10% microcrystalline Cellulose and 1%, mixing tabletting.
Embodiment 6 (preparation of drop pill)
Get Rhizoma Dioscoreae Bulbiferae 600g, Fructus Schisandrae Chinensis 200g, beat powder, with 70% alcohol reflux 3 times, each 2h reclaims ethanol and gets extractum, is substrate with PGE4000, with the ratio of medicine be 1: 1.5, dimethicone is a condensing agent, drips system.
Embodiment 7 (preparation of capsule)
Get Rhizoma Dioscoreae Bulbiferae 600g, Fructus Schisandrae Chinensis 200g, beat powder, extract 3 times with ethyl acetate backflow, each 2h reclaims ethyl acetate and gets extractum, and drying is pulverized, and crosses 100 mesh sieves, adds 5% magnesium stearate, the mixing hard capsule of packing into.
Claims (6)
1. the Chinese medicine composition with anti-curing cancers effect is characterized in that, it mainly is prepared from the following raw materials by weight percentage medicine: Rhizoma Dioscoreae Bulbiferae 20%～90%, Fructus Schisandrae Chinensis 10%～80%.
2. the Chinese medicine composition with anti-curing cancers effect according to claim 1; It is characterized in that; This Chinese medicine composition is full powder suspension, or water extract, 20%～95% ethanol extraction, acetone extract, ethyl acetate extract, gasoline or ligroin extraction.
3. the Chinese medicine composition with anti-curing cancers effect according to claim 2 is characterized in that, this Chinese medicine composition consist of Rhizoma Dioscoreae Bulbiferae diterpene lactone diosbulbin A-M and derivant and Fructus Schisandrae Chinensis lignin material.
4. the Chinese medicine composition with anti-curing cancers effect according to claim 3; It is characterized in that; The composition of this Chinese medicine composition and mass percent thereof are: diosbulbin B 50%～90%, schisandrin C, schisandrin, schisantherin B, schisantherin A, schisantherin B, schisantherin C, deoxyschizandrin and schisandrin B each 1%～50%.
5. according to any one described Chinese medicine composition among the claim 1-4, it is characterized in that this Chinese medicine composition is extractum, fluid extract, medicated wine, tincture, oral liquid, pill, powder, drop pill, tablet or capsule with anti-curing cancers effect.
6. any one described Chinese medicine composition is prevented and treated the application in hepatocarcinoma, gastric cancer, breast carcinoma, pulmonary carcinoma, bladder cancer, ovarian cancer, carcinoma of prostate, the esophageal carcinoma, cancer of pancreas, colorectal cancer, colon cancer, skin carcinoma, thyroid carcinoma, cervical cancer or the leukemic medicine in preparation among the claim 1-5.
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| CN108836963A (en) * | 2018-05-18 | 2018-11-20 | 山东省中医药研究院 | Application of the diosbulbin B in preparation treatment nodular goiter drug |
| CN114081873A (en) * | 2021-10-19 | 2022-02-25 | 广州医科大学附属第二医院 | Application of deoxyschizandrin |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103393795A (en) * | 2013-07-20 | 2013-11-20 | 石强 | Traditional Chinese medicine composition, preparation method and application thereof |
| CN108836963A (en) * | 2018-05-18 | 2018-11-20 | 山东省中医药研究院 | Application of the diosbulbin B in preparation treatment nodular goiter drug |
| CN108836963B (en) * | 2018-05-18 | 2020-06-05 | 山东省中医药研究院 | Application of diosbulbin B in preparation of drug for treating nodular goiter |
| CN114081873A (en) * | 2021-10-19 | 2022-02-25 | 广州医科大学附属第二医院 | Application of deoxyschizandrin |
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