CN102308787A - Cartilage preservation solution - Google Patents
Cartilage preservation solution Download PDFInfo
- Publication number
- CN102308787A CN102308787A CN201110188468A CN201110188468A CN102308787A CN 102308787 A CN102308787 A CN 102308787A CN 201110188468 A CN201110188468 A CN 201110188468A CN 201110188468 A CN201110188468 A CN 201110188468A CN 102308787 A CN102308787 A CN 102308787A
- Authority
- CN
- China
- Prior art keywords
- cartilage
- preservation
- preservation solution
- liquid
- histidine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a cartilage preservation solution. Each 1000ml of the preservation solution comprises 20-30mmol of potassium dihydrogen phosphate, 80mmol of histidine or 15mmol of a histidine salt, 75-95mmol of lactobionic acid, 50-70mmol of sucrose, 0.8-1.2mmol of allopurinol, 45-55g of low molecular dextran-40, 45-55mmol of NaCl, 25-35mmol of KCl, 3-7mmol magnesium sulfate, 70-90U of penicillin, 2-4mmol of reduced glutathione, 3-7mmol of adenosine, 2-5g of trehalose, and the balance deionized water, and the pH value of the cartilage preservation solution is 7.33-7.53. The cartilage preservation solution is packaged, sealed and preserved at 0-4DEG C. By adopting the preservation solution to preserve cartilage tissues, the death speed of cartilage cells is slowed, the activity of cartilage fibers is maintained, the performance of the cartilage tissues is kept, and the antibiotic property is increased, so the preservation solution has a good preservation effect and a long preservation time on the cartilage tissues, and is in favor of clinic cartilage tissue transplant operations.
Description
Technical field
The invention belongs to biomedical engineering field, be specifically related to a kind of cartilage that can solve the retrogression problem of implementing preservation back transfer operation articular cartilage of the same race and preserve liquid and preparation method thereof.
Background technology
Cartilage joint is as a complex tissue, and it preserves the influence that the biologically active of transplanting the back receives many factors, and the joint that causes because of wound, tumour or other cause of disease is damaged to be a difficult problem of treating in the medical science.Many scholars exploring a kind of method that can the midium or long term preserve cartilage joint of walk away safety, reach better situation and biologically active so that preserve biomethanics influence, cartilage cell and the vascular endothelial cell Change of Ultrastructure of back cartilage joint.
Along with the more and more participations of youngster's mouth in athletics sports and stress-relieving activity, the men and women of all age brackets possibly produce cartilage lesion, and the post-traumatic arthritis that can cause than more frequent in the past appearance cartilage lesion.If to the untimely treatment of this situation, may cause to one very the joint of young patient degenerate.Joint injury and the joint damage that is caused are often more serious in the elderly, and the patient can usually complain pain, swelling, can't carry out ADL or the like.United States Medicine discovers that the articular cartilage of infringement health is becoming the significant problem of American's LOM, comprises the professional athlete of five big sports unions.Even the progress of modern society makes the human life-span longer, but also can cause the misery of more permanent time simultaneously.
The artificial prosthesis substitution repairs the damaged effective means in joint, but is not suitable for the bigger adolescent patient of motion tension force, and adopting homogeneous allogenic bone and articular cartilage to transplant and rebuilding is one of orthopaedics method comparatively commonly used.But implement to carry out after cartilaginous tissue is preserved that the retrogression of articular cartilage of the same race is still a unavoidable problem after the transfer operation.
The midium or long term of walk away safety preserves cartilage liquid and has all obtained than much progress from basic theory and zoopery; Prepare through proportion of liquid; Fundamental mechanism to cell, fiber and tissue damage in the cartilaginous tissue preservation has had more deep understanding and solution; In the reaction and the dead speed that reduce edema, the cell glucose-6-phosphate dehydrogenase that slows down, prevent cell acidify, prevent that the space between cells from expanding, prevent or alleviating aspect the reperfusion injury all research experiment, and obtain effect preferably.The orthopedist can provide new selection for patient now clinically---the implantation reparation of cartilage defect, and for this type of patient brings glad tidings.But the preservation effect that on the storage temperature of cartilaginous tissue preservation liquid, still has low temperature is far longer than the preservation effect of normal temperature.
Summary of the invention
The object of the present invention is to provide a kind of retrogression pathological changes that can keep the BA of articular chondrocytes or delay articular cartilage, for clinical treatment large tracts of land articular cartilage defect, carry out cartilaginous tissue and transplant and to provide more cartilages that help to preserve liquid and preparation method thereof more.
For achieving the above object; The technical scheme that the present invention adopts is: cartilage preservation liquid phosphoric acid potassium dihydrogen 20-30mmol, histidine 80mmol or the histidine salt 15mmol of every 1000ml, lactobionic acid 75-95mmol, sucrose 50-70mmol, allopurinol 0.8-1.2mmol, D-40-40:45-55g, Nacl 45-55mmol, KCl 25-35mmol, magnesium sulfate 3-7mmol, penicillin 70-90U, reduced glutathione 2-4mmol, adenosine 3-7mmol, trehalose 2-5g, surplus are deionized water, and the pH value that this cartilage is preserved liquid is 7.33-7.53.
The present invention can overcome the difficult problem that biomethanics influence, cartilage cell and vascular endothelial cell Change of Ultrastructure and the biologically active of present stage cartilage joint in preserving descends, and is applicable to the issuable cartilage lesion of all age bracket crowds, and the post-traumatic arthritis that can cause than more frequent in the past appearance cartilage lesion and then develop into degenerative joint or damaged situation.This cartilage is preserved liquid can solve the problem of carrying out the retrogression of articular cartilage of the same race after the transfer operation after cartilaginous tissue is preserved preferably.
Description of drawings
Fig. 1 is that fresh sheep cartilaginous tissue is preserved liquid with the present invention and control treatment is carried out in traditional-80 ℃ of freezing preservations; Wherein Fig. 1 a preserves the electron micrograph in 1 week of fresh sheep cartilaginous tissue of liquid preservation for adopting the present invention, and Fig. 1 a` is for adopting 1 all electron micrographs of traditional-80 ℃ of fresh sheep cartilaginous tissues of freezing preservation; Fig. 1 b preserves the electron micrograph in 2 weeks of fresh sheep cartilaginous tissue of liquid preservation for adopting the present invention, and Fig. 1 b` is for adopting 2 all electron micrographs of traditional-80 ℃ of fresh sheep cartilaginous tissues of freezing preservation; Fig. 1 c preserves the electron micrograph in 3 weeks of fresh sheep cartilaginous tissue of liquid preservation for adopting the present invention, and Fig. 1 c` is for adopting 3 all electron micrographs of traditional-80 ℃ of fresh sheep cartilaginous tissues of freezing preservation; Fig. 1 d preserves the electron micrograph in 4 weeks of fresh sheep cartilaginous tissue of liquid preservation for adopting the present invention, and Fig. 1 d` is for adopting 4 all electron micrographs of traditional-80 ℃ of fresh sheep cartilaginous tissues of freezing preservation; Fig. 1 e preserves the electron micrograph in 5 weeks of fresh sheep cartilaginous tissue of liquid preservation for adopting the present invention, and Fig. 1 e` is for adopting 5 all electron micrographs of traditional-80 ℃ of fresh sheep cartilaginous tissues of freezing preservation.
Embodiment
Embodiment 1: the cartilage preservation liquid phosphoric acid potassium dihydrogen 20mmol of every 1000ml, histidine 80mmol, lactobionic acid 75mmol, sucrose 60mmol, allopurinol 1.0mmol, D-40-40:50g, Nacl 45mmol, KCl 35mmol, magnesium sulfate 5mmol, penicillin 75U, reduced glutathione 2.5mmol, adenosine 6mmol, trehalose 4g, surplus are deionized water, and the pH value that this cartilage is preserved liquid is 7.33-7.53.Packing is sealed, 0-4 ℃ of preservation.
Embodiment 2: the cartilage preservation liquid phosphoric acid potassium dihydrogen 30mmol of every 1000ml, histidine 80mmol, lactobionic acid 95mmol, sucrose 70mmol, allopurinol 1.2mmol, D-40-40:55g, Nacl 55mmol, KCl 25mmol, magnesium sulfate 7mmol, penicillin 90U, reduced glutathione 3mmol, adenosine 5mmol, trehalose 5g, surplus are deionized water, and the pH value that this cartilage is preserved liquid is 7.33-7.53.Packing is sealed, 0-4 ℃ of preservation.
Embodiment 3: the cartilage preservation liquid phosphoric acid potassium dihydrogen 25mmol of every 1000ml, histidine 80mmol, lactobionic acid 85mmol, sucrose 50mmol, allopurinol 0.8mmol, D-40-40:45g, Nacl 48mmol, KCl 30mmol, magnesium sulfate 4mmol, penicillin 80U, reduced glutathione 4mmol, adenosine 7mmol, trehalose 2g, surplus are deionized water, and the pH value that this cartilage is preserved liquid is 7.33-7.53.Packing is sealed, 0-4 ℃ of preservation.
Embodiment 4: the cartilage preservation liquid phosphoric acid potassium dihydrogen 23mmol of every 1000ml, histidine salt 15mmol, lactobionic acid 80mmol, sucrose 55mmol, allopurinol 1.1mmol, D-40-40:48g, Nacl 50mmol, KCl 32mmol, magnesium sulfate 6mmol, penicillin 70U, reduced glutathione 3.5mmol, adenosine 3mmol, trehalose 3g, surplus are deionized water, and the pH value that this cartilage is preserved liquid is 7.33-7.53.Packing is sealed, 0-4 ℃ of preservation.
Embodiment 5: the cartilage preservation liquid phosphoric acid potassium dihydrogen 26mmol of every 1000ml, histidine salt 15mmol, lactobionic acid 90mmol, sucrose 65mmol, allopurinol 0.9mmol, D-40-40:52g, Nacl 53mmol, KCl 27mmol, magnesium sulfate 3mmol, penicillin 85U, reduced glutathione 2mmol, adenosine 4mmol, trehalose 2g, surplus are deionized water, and the pH value that this cartilage is preserved liquid is 7.33-7.53.Packing is sealed, 0-4 ℃ of preservation.
Embodiment 6: the cartilage preservation liquid phosphoric acid potassium dihydrogen 28mmol of every 1000ml, histidine salt 15mmol, lactobionic acid 88mmol, sucrose 63mmol, allopurinol 1.0mmol, D-40-40:50g, Nacl 46mmol, KCl29mmol, magnesium sulfate 4mmol, penicillin 82U, reduced glutathione 3mmol, adenosine 5mmol, trehalose 4g, surplus are deionized water, and the pH value that this cartilage is preserved liquid is 7.33-7.53.Packing is sealed, 0-4 ℃ of preservation.
Potassium dihydrogen phosphate, histidine or histidine hydrochloric acid are right as efficient buffer among the present invention, to prevent cell acidify; Allopurinol and D-40-40 prevents that the space between cells from expanding, the too much bypass between reducing effectively from the capillary to the ECS, thereby the composition transportation that has guaranteed to preserve liquid; Nacl, KCl and magnesium sulfate reduce edema and lower cell mortality; Reduced glutathione and adenosine are used for the substrate that the active fast quick-recovery of sodium pump and anti-oxidant and cell provide energy (ATP), for the reparation and the activation energy utilization of reperfusion injury are reacted; Trehalose provides better protection to muscle ligament, is beneficial to operation transplantation.Adjust pH to 7.43 ± 0.10 makes preserves liquid and is alkalescent, 0-4 and ℃ preserves to be beneficial to and alleviate the tissue reperfusion damage.
Referring to Fig. 1 fresh sheep cartilaginous tissue is carried out control treatment with preservation liquid of the present invention and traditional-80 ℃ of freezing preservations; Choosing 1 week, 2 weeks, 3 weeks, 4 weeks and 5 time-of-weeks more respectively contrasts; The result who adopts electron microscope to take pictures and obtain: visible Fig. 1 a of first week of experiment (cartilage is preserved the liquid preservation method) cartilage cell is more obvious, and the cartilage cell is high-visible.The visible down cartilage cavities that differs in size in Fig. 1 a` (traditional freeze preservation) visual field has preliminary variation.
Test second all Fig. 1 b (cartilage is preserved the liquid preservation method) it is thus clear that the chondrigen fiber is thick, it is tight to arrange, be layered arrangement.The visible cartilage fibres of Fig. 1 b` (traditional freeze preservation) begins to occur arranging the phenomenon of moving towards unordered.
Test the visible cartilage cavities of the 3rd all Fig. 1 c (cartilage preserve liquid preservation method) and partly disappear, but the chondrigen fiber is arranged in parallel, arrange more neat.Buckling phenomenon appears in the visible collagenous fibres of Fig. 1 c` (traditional freeze preservation).
It is orderly to test the visible collagenous fibres mid portion arrangement of Fig. 1 d (cartilage is preserved the liquid preservation method) all around, marginal portion had slight disorder phenomenon.The visible collagenous fibres buckling phenomenon of Fig. 1 d` (traditional freeze preservation) is more obvious, and the part arrangement of collagen fibers is disorderly.
Test the visible collagenous fibres of the 5th all Fig. 1 e (cartilage is preserved the liquid preservation method) and the diagonal bending occurs, the fracture of Fig. 1 e` (traditional freeze preservation) visible surface collagenous fibres, the arrangement disorder situation is more serious.
Take a picture by electron microscope and can know and find out; Variation along with the holding time; The cartilage surface structure gradually by smoothly become out-of-flatness, occur damaged; It is the most obvious that the cartilage surface collagenous fibres change, collagenous fibres by marshalling, disorderly, the fracture that becomes in order, cartilage cavities fades away simultaneously.But because the difference of store method, the experimental group pace of change of being preserved the liquid preservation by the present invention is significantly less than traditional freezing method preservation group.Corresponding is that the retention cycle of cartilaginous tissue is elongated thereupon, and the cartilage transplantation aspect in the clinical treatment is had certain meaning.
Claims (1)
1. a cartilage is preserved liquid; It is characterized in that: cartilage preservation liquid phosphoric acid potassium dihydrogen 20-30mmol, histidine 80mmol or the histidine salt 15mmol of every 1000ml, lactobionic acid 75-95mmol, sucrose 50-70mmol, allopurinol 0.8-1.2mmol, D-40-40:45-55g, Nacl45-55mmol, KCl 25-35mmol, magnesium sulfate 3-7mmol, penicillin 70-90U, reduced glutathione 2-4mmol, adenosine 3-7mmol, trehalose 2-5g, surplus are deionized water, and the pH value that this cartilage is preserved liquid is 7.33-7.53.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110188468 CN102308787B (en) | 2011-07-06 | 2011-07-06 | Cartilage preservation solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110188468 CN102308787B (en) | 2011-07-06 | 2011-07-06 | Cartilage preservation solution |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102308787A true CN102308787A (en) | 2012-01-11 |
| CN102308787B CN102308787B (en) | 2013-10-02 |
Family
ID=45422658
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110188468 Expired - Fee Related CN102308787B (en) | 2011-07-06 | 2011-07-06 | Cartilage preservation solution |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102308787B (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104938478A (en) * | 2015-05-20 | 2015-09-30 | 泰山医学院 | Cartilago articularis vitrification cryoprotectant and cartilage preservation method |
| CN104938476A (en) * | 2015-03-02 | 2015-09-30 | 泰山医学院 | Osteochondral graft preservation liquid and preparation method thereof |
| CN106614526A (en) * | 2016-12-30 | 2017-05-10 | 潍坊医学院 | Vitrified cryopreservation method for cartilage |
| CN108835105A (en) * | 2018-09-10 | 2018-11-20 | 刘亚莉 | A kind of articular cartilage glass freezing protection liquid and preparation method |
| CN109392892A (en) * | 2018-12-14 | 2019-03-01 | 广州悦清再生医学科技有限公司 | A kind of biological collagen sill saves liquid and application |
| CN109566601A (en) * | 2018-12-29 | 2019-04-05 | 中国人民解放军第四军医大学 | A kind of cartilage preservation solution and its application method |
| CN109644989A (en) * | 2019-01-28 | 2019-04-19 | 徐艳红 | A kind of articular cartilage frozen solution and cartilage freezing and storing method |
| CN110558312A (en) * | 2019-10-09 | 2019-12-13 | 科瑞百奥泰州生物技术有限公司 | Cartilage tissue low-temperature refrigeration preservation solution and application thereof |
| CN111657267A (en) * | 2020-06-17 | 2020-09-15 | 科瑞百奥泰州生物技术有限公司 | Ice-free crystal frozen preservation solution and freezing method for preservation of cartilage, tendon and meniscus |
| CN113767895A (en) * | 2021-09-23 | 2021-12-10 | 北京大麦植发技术研究有限公司 | Composite hair follicle tissue preservation solution, preparation method thereof and preservation method for maintaining hair follicle in-vitro activity |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6365338B1 (en) * | 1999-04-27 | 2002-04-02 | David A. Bull | Organ preservative solution containing trehalose, anti-oxidant, cations and an energy source |
| CN101098622A (en) * | 2004-11-12 | 2008-01-02 | 多尔灿德气动股份有限公司 | Composition for cold preservation and perfusion of organs |
-
2011
- 2011-07-06 CN CN 201110188468 patent/CN102308787B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6365338B1 (en) * | 1999-04-27 | 2002-04-02 | David A. Bull | Organ preservative solution containing trehalose, anti-oxidant, cations and an energy source |
| CN101098622A (en) * | 2004-11-12 | 2008-01-02 | 多尔灿德气动股份有限公司 | Composition for cold preservation and perfusion of organs |
Non-Patent Citations (1)
| Title |
|---|
| 郑军华: "长征-1号多器官保存液研制中的哲学思考", 《医学与哲学》 * |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104938476A (en) * | 2015-03-02 | 2015-09-30 | 泰山医学院 | Osteochondral graft preservation liquid and preparation method thereof |
| CN104938476B (en) * | 2015-03-02 | 2019-04-09 | 泰山医学院 | A kind of osteochondral graft saves liquid and preparation method thereof |
| CN104938478B (en) * | 2015-05-20 | 2018-02-02 | 泰山医学院 | A kind of articular cartilage glass freezing protection liquid and cartilage store method |
| CN104938478A (en) * | 2015-05-20 | 2015-09-30 | 泰山医学院 | Cartilago articularis vitrification cryoprotectant and cartilage preservation method |
| CN106614526A (en) * | 2016-12-30 | 2017-05-10 | 潍坊医学院 | Vitrified cryopreservation method for cartilage |
| CN108835105A (en) * | 2018-09-10 | 2018-11-20 | 刘亚莉 | A kind of articular cartilage glass freezing protection liquid and preparation method |
| CN109392892B (en) * | 2018-12-14 | 2021-07-23 | 广州悦清再生医学科技有限公司 | Biological collagen-based material preserving fluid and application thereof |
| CN109392892A (en) * | 2018-12-14 | 2019-03-01 | 广州悦清再生医学科技有限公司 | A kind of biological collagen sill saves liquid and application |
| CN109566601A (en) * | 2018-12-29 | 2019-04-05 | 中国人民解放军第四军医大学 | A kind of cartilage preservation solution and its application method |
| CN109644989A (en) * | 2019-01-28 | 2019-04-19 | 徐艳红 | A kind of articular cartilage frozen solution and cartilage freezing and storing method |
| CN110558312A (en) * | 2019-10-09 | 2019-12-13 | 科瑞百奥泰州生物技术有限公司 | Cartilage tissue low-temperature refrigeration preservation solution and application thereof |
| CN111657267A (en) * | 2020-06-17 | 2020-09-15 | 科瑞百奥泰州生物技术有限公司 | Ice-free crystal frozen preservation solution and freezing method for preservation of cartilage, tendon and meniscus |
| CN111657267B (en) * | 2020-06-17 | 2021-02-02 | 科瑞百奥泰州生物技术有限公司 | Ice-free crystal frozen preservation solution and freezing method for preservation of cartilage, tendon and meniscus |
| CN113767895A (en) * | 2021-09-23 | 2021-12-10 | 北京大麦植发技术研究有限公司 | Composite hair follicle tissue preservation solution, preparation method thereof and preservation method for maintaining hair follicle in-vitro activity |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102308787B (en) | 2013-10-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102308787B (en) | Cartilage preservation solution | |
| JP2859925B2 (en) | Cell resuscitation method before cryopreservation | |
| Schwartz | Cryopreservation as long-term storage of teeth for transplantation or replantation | |
| CN1477926A (en) | Evaluation and preservation solution | |
| IL101637A0 (en) | Aqueous solutions for perfusion | |
| ES2834388T3 (en) | Modulation of calcium ion homeostasis in harvested transplantable hearts | |
| CN103272282A (en) | Nanometer hydroxyapatite/chitosan/fish skin collagen composite scaffold | |
| CN102438445A (en) | Method for ice-free cryopreservation of tissue | |
| CA2362051A1 (en) | Methods and compositions for use in perfusion applications | |
| CA2593161A1 (en) | Pharmaceutical composition and method for regenerating myofibers in the treatment of muscle injuries | |
| Zhai et al. | Natural zwitterionic l-Carnitine as efficient cryoprotectant for solvent-free cell cryopreservation | |
| Bonfiglio | Repair of bone-transplant fractures | |
| EP1677594B1 (en) | Method for freezing, thawing and transplantation of viable cartilage | |
| CN108338160B (en) | Hair follicle preservation solution and preparation method thereof | |
| CN109566601A (en) | A kind of cartilage preservation solution and its application method | |
| CN207270386U (en) | A kind of portable hair transplant purpose-made pallet | |
| CN104622772A (en) | Medical cosmetic product containing autologous peripheral blood vascular endothelial progenitor cells | |
| Premaratne et al. | Repeated implantation is a more effective cell delivery method in skeletal myoblast transplantation for rat myocardial infarction | |
| Yaneselli et al. | Allogeneic stem cell transplantation for bone regeneration of a nonunion defect in a canine | |
| CN210330755U (en) | Novel hair follicle is planted and is sent out and use tray | |
| Kiehn et al. | A study of the viability of autogenous frozen bone grafts by means of radioactive phosphorus | |
| JP4590041B2 (en) | Organ preservative | |
| Vekemans et al. | Hypothermic liver machine perfusion with EKPS-1 solution vs Aqix RS-I solution: in vivo feasibility study in a pig transplantation model | |
| Lin et al. | Prolonged cold-ischemia increases reactive oxygen species and activates autophagy by enhancing glycolysis in the graft through the mTOR signaling pathway in orthotopic lung transplantation of rats | |
| CN1528469A (en) | Method for preparing and preserving homogeneous bone tissue with biological activity |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C53 | Correction of patent of invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: He Xiaole Inventor after: Liu Jun Inventor after: Bu Weiping Inventor after: Wang Zhen Inventor after: Jin Zhongmin Inventor after: Lian Ling Inventor after: Guo Zheng Inventor after: Zhang Yu Inventor before: Wang Zhen Inventor before: Liu Jun Inventor before: He Xiaole Inventor before: Jin Zhongmin Inventor before: Lian Ling Inventor before: Guo Zheng Inventor before: Zhang Yu |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: WANG ZHEN LIU JUN HE XIAOLE JIN ZHONGMIN LIAN GUO ZHENG ZHANG YU TO: HE XIAOLE LIU JUN BU WEIPING WANG ZHEN JIN ZHONGMIN LIAN GUO ZHENG ZHANG YU |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131002 Termination date: 20150706 |
|
| EXPY | Termination of patent right or utility model |