CN102297963A - Premature rupture of membrane colloidal gold test paper reaction buffer, and preparation method thereof - Google Patents
Premature rupture of membrane colloidal gold test paper reaction buffer, and preparation method thereof Download PDFInfo
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- CN102297963A CN102297963A CN2011101411017A CN201110141101A CN102297963A CN 102297963 A CN102297963 A CN 102297963A CN 2011101411017 A CN2011101411017 A CN 2011101411017A CN 201110141101 A CN201110141101 A CN 201110141101A CN 102297963 A CN102297963 A CN 102297963A
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Abstract
The invention discloses a premature rupture of membrane colloidal gold test paper reaction buffer, and a preparation method thereof. The method comprises the following steps: dissolving 1.2-7.8 parts of trisodium citrate in 100 parts of purified water to prepare a solution A; dissolving 0.1-1.2 parts of citric acid in 100 parts of purified water to prepare a solution B; adjusting the pH value of the solution A to 6.50-10.00 through the solution B to prepare a sodium citrate buffer; adding 1-5 parts of BSA, 0.5-0.9 parts of NaCl, 0.2-2 parts of TritonX100, 0.01-0.06 parts of Proclin300 to the 100 parts of the sodium citrate buffer, uniformly stirring to prepare the premature rupture of membrane colloidal gold test paper reaction buffer. According to the present invention, the migration speed of the colloidal gold test paper can be substantially increased; the emergence times of the detection line and the quality control line can be shortened; the components of the buffer are common; the prices of the components of the buffer are relatively cheap; the preparation method is more convenient; the preparation process is simple and easy to operate.
Description
Technical field
The invention belongs to medical detection range, specifically, relate to a kind of reaction buffer of medical external diagnosis reagent.
Background technology
Colloidal gold strip belongs to the immunology detection category, and method is special, and susceptibility is good.Romano in 1974 etc. on second antibody (the anti-human IgG of horse), have set up indirect immune colloid gold decoration method with colloid gold label.Geoghega had found the application of colloid gold label thing in the light microscopic level in 1978.This application of collaurum in immunochemistry, the immunity gold is otherwise known as. afterwards, many scholars confirm that further collaurum can stablize again adsorbed proteins promptly, and the biologically active of protein does not have obvious change. it can be used as the accurate location that probe carries out biomacromolecules such as polysaccharide, protein, many skins, antigen, hormone, nucleic acid in cell surface and the cell, also can be used for daily immunodiagnosis, carry out the immunohistochemistry location, thereby the application at aspects such as clinical diagnosis and drug tests has been subjected to paying attention to widely.The premature rupture of fetal membranes colloid gold test paper is formed in conjunction with the corresponding antibodies of albumen-1 and control zone (C) by containing by the anti-insulin like growth factor of predetermined fixed detection zone (T) on film, be mainly used in external fast qualitative and detect amniotic fluid insulin-like growth factor binding protein-1 in pregnant woman's vaginal fluid, it is under a cloud or have clinical sign showing that premature rupture of fetal membranes may take place to be used for auxiliary diagnosis.But at present, be used for the damping fluid of premature rupture of fetal membranes colloid gold test paper reaction, the fast inadequately and nature controlling line of the speed of dividing a word with a hyphen at the end of a line, that speed appears in detection line is slower.
Summary of the invention
For solving above technical matters, it is a kind of with low cost that one of the object of the invention is to provide, and can improve the colloid gold test paper speed of dividing a word with a hyphen at the end of a line greatly, shortens the premature rupture of fetal membranes colloid gold test paper reaction buffer of detection line, nature controlling line time of occurrence.
Two of the object of the invention is to provide a kind of preparation method of premature rupture of fetal membranes colloid gold test paper reaction buffer.
The present invention seeks to realize like this:
A kind of premature rupture of fetal membranes colloid gold test paper reaction buffer is made up of following raw materials according by weight:
The pH value is 100 parts of the sodium citrate buffer solutions of pH6.50～10.00
1～5 part of BSA (bovine serum albumin)
Nacl 0.5-0.9 part
0.2～2 part of TritonX100
Proclin300 0.01-0.06 part.
Above-mentioned sodium citrate buffer solution is dissolved in 100 parts of purified water by 1.2～7.8 parts of trisodium citrates by weight, wiring solution-forming A; Citric acid is dissolved in 100 parts of purified water wiring solution-forming B for 0.1～1.2 part; Transfer solution A to pH6.50～10.00 to obtain with solution B.
The binding ability that the sodium citrate buffer solution of potential of hydrogen is used to improve antigen-antibody in the damping fluid improves the color speed of colour band, use TritonX100 as non-ionic surfactant, add roll booster to the material in the reaction systems such as albumen, accelerated chromatography speed, the use of BSA makes reaction system more near the system of the secretion in the vagina, adds Proclin300 and makes damping fluid avoid microorganism such as breed bacteria to play antisepsis.
A kind of method for preparing premature rupture of fetal membranes colloid gold test paper reaction buffer, its key are to carry out as follows:
(1) takes by weighing 1.2～7.8 parts of trisodium citrates and be dissolved in 100 parts of purified water wiring solution-forming A; Take by weighing 0.1～1.2 part of citric acid and be dissolved in 100 parts of purified water wiring solution-forming B; Transfer solution A to pH6.50～10.00 with solution B, be made into sodium citrate buffer solution;
(2) measure 100 parts of sodium citrate buffer solutions, take by weighing 1～5 part of BSA, 0.5～0.9 part of Nacl, 0.2～2 part of TritonX100, Proclin300 adds in the sodium citrate buffer solution for 0.01～0.06 part, stirs, and is made into premature rupture of fetal membranes colloid gold test paper reaction buffer.
Beneficial effect: the present invention can improve the colloid gold test paper speed of dividing a word with a hyphen at the end of a line greatly, shorten detection line, nature controlling line time of occurrence, and the damping fluid constituent is common, and price is relatively cheap, and whole preparation is also more convenient, the simple operation easily of preparation section.
Embodiment
Embodiment 1
(1) takes by weighing trisodium citrate 1.2 grams and be dissolved in 100 milliliters of purified water wiring solution-forming A; Take by weighing citric acid 0.1 gram and be dissolved in 100 milliliters of purified water wiring solution-forming B; Transfer solution A to pH6.50 with solution B, wiring solution-forming C
(2) measure 100 milliliters of solution C, take by weighing BSA 1 gram, Nacl 0.5 gram, TritonX100 0.2 gram, Proclin300 0.01ml adds in the solution C, stirs, and is made into premature rupture of fetal membranes colloid gold test paper reaction buffer;
Embodiment 2
(1) takes by weighing trisodium citrate 1.5 grams and be dissolved in 100 milliliters of purified water wiring solution-forming A; Take by weighing citric acid 0.2 gram and be dissolved in 100 milliliters of purified water wiring solution-forming B; Transfer solution A to pH7.00 with solution B, wiring solution-forming C
(2) measure 100 milliliters of solution C, take by weighing BSA 1.5 grams, Nacl 0.6 gram, TritonX100 0.3 gram, Proclin300 0.02ml adds in the solution C, stirs, and is made into premature rupture of fetal membranes colloid gold test paper reaction buffer;
Embodiment 3
(1) takes by weighing trisodium citrate 2.0 grams and be dissolved in 100 milliliters of purified water wiring solution-forming A; Take by weighing citric acid 0.4 gram and be dissolved in 100 milliliters of purified water wiring solution-forming B; Transfer solution A to pH7.50 with solution B, wiring solution-forming C
(2) measure 100 milliliters of solution C, take by weighing the BSA2.0 gram, Nacl 0.8 gram, TritonX100 0.5 gram, Proclin300 0.03ml adds in the solution C, stirs, and is made into premature rupture of fetal membranes colloid gold test paper reaction buffer;
Embodiment 4
(1) takes by weighing trisodium citrate 2.5 grams and be dissolved in 100 milliliters of purified water wiring solution-forming A; Take by weighing citric acid 0.6 gram and be dissolved in 100 milliliters of purified water wiring solution-forming B; Transfer solution A to pH8.00 with solution B, wiring solution-forming C
(2) measure 100 milliliters of solution C, take by weighing the BSA3.0 gram, Nacl 0.9 gram, TritonX100 0.8 gram, Proclin300 0.04ml adds in the solution C, stirs, and is made into premature rupture of fetal membranes colloid gold test paper reaction buffer;
Embodiment 5
(1) takes by weighing trisodium citrate 3.0 grams and be dissolved in 100 milliliters of purified water wiring solution-forming A; Take by weighing citric acid 0.8 gram and be dissolved in 100 milliliters of purified water wiring solution-forming B; Transfer solution A to pH8.50 with solution B, wiring solution-forming C
(2) measure 100 milliliters of solution C, take by weighing the BSA4.0 gram, Nacl 0.9 gram, TritonX100 1.0 grams, Proclin300 0.05ml adds in the solution C, stirs, and is made into premature rupture of fetal membranes colloid gold test paper reaction buffer;
Embodiment 6
(1) takes by weighing trisodium citrate 4.0 grams and be dissolved in 100 milliliters of purified water wiring solution-forming A; Take by weighing citric acid 1.0 grams and be dissolved in 100 milliliters of purified water wiring solution-forming B; Transfer solution A to pH9.0 with solution B, wiring solution-forming C
(2) measure 100 milliliters of solution C, take by weighing the BSA5.0 gram, Nacl 0.9 gram, TritonX100 1.1 grams, Proclin300 0.06ml adds in the solution C, stirs, and is made into premature rupture of fetal membranes colloid gold test paper reaction buffer;
Embodiment 7
(1) takes by weighing trisodium citrate 5.0 grams and be dissolved in 100 milliliters of purified water wiring solution-forming A; Take by weighing citric acid 1.2 grams and be dissolved in 100 milliliters of purified water wiring solution-forming B; Transfer solution A to pH9.5 with solution B, wiring solution-forming C
(2) measure 100 milliliters of solution C, take by weighing the BSA5.0 gram, Nacl 0.9 gram, TritonX100 1.5 grams, Proclin300 0.06ml adds in the solution C, stirs, and is made into premature rupture of fetal membranes colloid gold test paper reaction buffer;
Embodiment 8
(1) takes by weighing trisodium citrate 5.5 grams and be dissolved in 100 milliliters of purified water wiring solution-forming A; Take by weighing citric acid 1.2 grams and be dissolved in 100 milliliters of purified water wiring solution-forming B; Transfer solution A to pH9.8 with solution B, wiring solution-forming C
(2) measure 100 milliliters of solution C, take by weighing the BSA5.0 gram, Nacl 0.9 gram, TritonX100 1.8 grams, Proclin300 0.06ml adds in the solution C, stirs, and is made into premature rupture of fetal membranes colloid gold test paper reaction buffer;
Embodiment 9
(1) takes by weighing trisodium citrate 6.0 grams and be dissolved in 100 milliliters of purified water wiring solution-forming A; Take by weighing citric acid 1.2 grams and be dissolved in 100 milliliters of purified water wiring solution-forming B; Transfer solution A to pH10.0 with solution B, wiring solution-forming C
(2) measure 100 milliliters of solution C, take by weighing the BSA5.0 gram, Nacl 0.9 gram, TritonX100 2.0 grams, Proclin300 0.06ml adds in the solution C, stirs, and is made into premature rupture of fetal membranes colloid gold test paper reaction buffer;
Embodiment 10
(1) takes by weighing trisodium citrate 6.5 grams and be dissolved in 100 milliliters of purified water wiring solution-forming A; Take by weighing citric acid 1.0 grams and be dissolved in 100 milliliters of purified water wiring solution-forming B; Transfer solution A to pH10.0 with solution B, wiring solution-forming C
(2) measure 100 milliliters of solution C, take by weighing the BSA5.0 gram, Nacl 0.9 gram, TritonX100 1.5 grams, Proclin300 0.05ml adds in the solution C, stirs, and is made into premature rupture of fetal membranes colloid gold test paper reaction buffer;
Embodiment 11
(1) takes by weighing trisodium citrate 7.0 grams and be dissolved in 100 milliliters of purified water wiring solution-forming A; Take by weighing citric acid 1.0 grams and be dissolved in 100 milliliters of purified water wiring solution-forming B; Transfer solution A to pH10.0 with solution B, wiring solution-forming C
(2) measure 100 milliliters of solution C, take by weighing the BSA4.0 gram, Nacl 0.9 gram, TritonX100 1.2 grams, Proclin300 0.04ml adds in the solution C, stirs, and is made into premature rupture of fetal membranes colloid gold test paper reaction buffer;
Embodiment 12
(1) takes by weighing trisodium citrate 7.5 grams and be dissolved in 100 milliliters of purified water wiring solution-forming A; Take by weighing citric acid 1.0 grams and be dissolved in 100 milliliters of purified water wiring solution-forming B; Transfer solution A to pH10.0 with solution B, wiring solution-forming C
(2) measure 100 milliliters of solution C, take by weighing the BSA5.0 gram, Nacl 0.85 gram, TritonX100 1.2 grams, Proclin300 0.05ml adds in the solution C, stirs, and is made into premature rupture of fetal membranes colloid gold test paper reaction buffer;
Embodiment 13
(1) takes by weighing trisodium citrate 7.80 grams and be dissolved in 100 milliliters of purified water wiring solution-forming A; Take by weighing citric acid 1.2 grams and be dissolved in 100 milliliters of purified water wiring solution-forming B; Transfer solution A to pH10.0 with solution B, wiring solution-forming C
(2) measure 100 milliliters of solution C, take by weighing the BSA4.50 gram, Nacl 0.85 gram, TritonX100 1.0 grams, Proclin300 0.05ml adds in the solution C, stirs, and is made into premature rupture of fetal membranes colloid gold test paper reaction buffer;
Embodiment 14
(1) takes by weighing trisodium citrate 7.50 grams and be dissolved in 100 milliliters of purified water wiring solution-forming A; Take by weighing citric acid 1.0 grams and be dissolved in 100 milliliters of purified water wiring solution-forming B; Transfer solution A to pH10.0 with solution B, wiring solution-forming C
(2) measure 100 milliliters of solution C, take by weighing the BSA4.00 gram, Nacl 0.75 gram, TritonX100 0.9 gram, Proclin300 0.05ml adds in the solution C, stirs, and is made into premature rupture of fetal membranes colloid gold test paper reaction buffer;
Table 1 is the part of detecting result of embodiment of the invention 1-14
The raw material that is used in the table 1 test is respectively:
Insulin-like growth factor binding protein I antigen: insulin-like growth factor binding protein I(U.S. Meridian company produces)
The premature rupture of fetal membranes colloid gold test paper: insulin-like growth factor binding protein I measures kit (Chongqing Zhongyuan Biological Technology Co., Ltd.'s production)
BSA:Roche company produces
During test insulin-like growth factor binding protein I is added in the premature rupture of fetal membranes colloid gold test paper reaction buffer, detect, carry out data analysis then with the premature rupture of fetal membranes colloid gold test paper.
Table 1: test result
Claims (3)
1. a premature rupture of fetal membranes colloid gold test paper reaction buffer is made up of following raw materials according by weight:
The pH value is 100 parts of the sodium citrate buffer solutions of pH6.50～10.00
1～5 part of BSA
Nacl 0.5-0.9 part
0.2～2 part of TritonX100
0.01～0.06 part of Proclin300.
2. according to the described a kind of premature rupture of fetal membranes colloid gold test paper reaction buffer of claim 1, it is characterized in that: described sodium citrate buffer solution is dissolved in 100 parts of purified water by 1.2～7.8 parts of trisodium citrates by weight, wiring solution-forming A; Citric acid is dissolved in 100 parts of purified water wiring solution-forming B for 0.1～1.2 part; Transfer solution A to pH6.50～10.00 to obtain with solution B.
3. method for preparing claim 1 or 2 described premature rupture of fetal membranes colloid gold test paper reaction buffers is characterized in that carrying out as follows:
(1) takes by weighing 1.2～7.8 parts of trisodium citrates and be dissolved in 100 parts of purified water wiring solution-forming A; Take by weighing 0.1～1.2 part of citric acid and be dissolved in 100 parts of purified water wiring solution-forming B; Transfer solution A to pH6.50～10.00 with solution B, be made into sodium citrate buffer solution;
(2) measure 100 parts of sodium citrate buffer solutions, take by weighing 1～5 part of BSA, 0.5～0.9 part of Nacl, 0.2～2 part of TritonX100, Proclin300 adds in the sodium citrate buffer solution for 0.01～0.06 part, stirs, and is made into premature rupture of fetal membranes colloid gold test paper reaction buffer.
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Cited By (1)
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| CN105699370A (en) * | 2016-01-20 | 2016-06-22 | 江西华清博恩生物科技有限公司 | Amniotic fluid detection indicator composition, preparation thereof and corresponding amniotic fluid detection pad |
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Application publication date: 20111228 |