CN102296103A

CN102296103A - Solid-phase pour enrichment method and matched device thereof

Info

Publication number: CN102296103A
Application number: CN2010102109759A
Authority: CN
Inventors: 唐锋
Original assignee: Individual
Current assignee: Individual
Priority date: 2010-06-28
Filing date: 2010-06-28
Publication date: 2011-12-28

Abstract

The invention relates to a solid-phase pour enrichment method which is characterized by using a solid-phase enrichment medium and a swab containing the solid-phase enrichment medium instead of a liquid-phase enrichment fluid, and carrying out pour cultivation to culture and separate pathogen from an original sample containing miscellaneous bacteria. The matched device of the solid-phase pour enrichment method comprises a container containing the solid-phase enrichment medium and a swab containing the medium, thereby simultaneously having the functions of sampling, preenrichment, enrichment and biochemical coarse screening. By taking a salmonella detector tube for physical examination of employees as an example, the solid-phase pour enrichment method comprises the following steps: wetting a anal swab containing the medium with water, directly sampling before culturing, and directly observing the reaction; and if salmonella is suspected to exist and a colored suspicious spot is presented on the swab, taking the spot for pure separation, and carrying out further identification. Having no spot is a representative of negative and leads to no need of flat plate subcultivation. The method has a significantly improved detection rate in comparison with the conventional method; and as the observation time can be prolonged, slow-growing bacteria can not be neglected. The method has the advantages of simplified procedures, lowered cost and high detection rate. The invention also discloses a salmonella detector bag using the same principle, which has a higher detection rate. Besides, a series of multi-function products can be developed, such as a series of products used for food bacterium monitoring, food poisoning detection, infectious disease detection, hospital infection monitoring and the like.

Description

Solid phase is poured into enrichment and the supporting device of using

Technical field

The present invention relates to a kind of laboratory and pour into enrichment and the supporting device of using with microbial culture method and proofing unit, particularly solid phase.

Background technology

At present during bacterial detection, adopt the swab sampling more, increase bacterium with the liquid enrichment liquid again.The swab of its sampling usefulness transfers in physiological saline or the enrichment liquid behind the sticking sample thief only as a kind of launch vehicle.The Salmonellas examination of carrying out during such as practitioner's health check-up, traditional method be with anus wipe away sampling be placed on increase bacterium in the enrichment liquid after, flat the pulling of transferred species done pure separation again, selects suspicious bacterium colony then and does next step evaluation.This traditional method is little but formality is numerous and diverse, and there is the more improved problem for the treatment of in the liquid enrichment liquid, as consider to help as yet injured thalline, in and antibiotic in the sample, to the problems such as time-delay observation of slow growth bacterium, and the more important thing is the minimum sample segment that next step can only utilize previous step, randomness is big, representative poor, so recall rate is extremely low, and no matter has or not suspicious Salmonellas in the enrichment liquid, each sample all wants the transferred species flat board to carry out pure separation, causes waste.For separating time the problem that exists, though once applied for utility model patent (patent No. ZL 2007200879654), only be the improvement of shape and structure, fail fundamentally to solve the problem of above-mentioned existence.

Summary of the invention

For addressing the above problem, especially the low problem of recall rate is developed solid phase and is poured into enrichment and the supporting device of using.Technical scheme of the present invention is to adopt solid phase to pour into enrichment, utilize the principle of pour plate method, the solid phase of development is poured into enrichment medium and reagent is added in the various containers such as test tube, flat board, reagent bottle, plastics bag, pour into cultivation after adding sample, also substratum can be added on the swab with absorbent function the direct inoculation primary sample.For easy to operate, characteristics at different samples, the spy makes various shape with swab (4,5), and can set up various difform handles (1,2,3), can form a complete set of into detector tube with corresponding container (6,7,8) simultaneously, detects bag, detect flat board.Swab is after so handling, and adding water can directly use, and after the sampling, the bacterium in the sample will penetrate into swab inside located growth separately, similarly pours into cultivation.In view of the above and a kind of practitioner of design carries disease germs and checks that special-purpose anus wipes away, the long 6～20cm of its handle (1), the long 1～10cm of hypomere swab (4) contains solid phase to pour into enrichment special culture media and reagent, adds the water post-sampling, cultivates the back observing response.If have suspiciously, continue to do isolation identification, as do not have suspiciously, then do negative the processing, needn't the transferred species flat board.After handling by this method, whole samples that anus is wiped away all will obtain utilizing, no any losing, and the unfavorable factors such as antibiotic that the while grows in bacterium and the sample at injured thalline, have late been taked corresponding measure, so recall rate obviously improves.Be example still, get final product observing response after directly cultivating with special-purpose swab sampling with the Salmonellas detection.This method with the sampling in the testing procedures, precedingly increase bacterium, increase bacterium and cultivate, separate several big steps of primary dcreening operation and be merged into a step and finish, the recall rate of not only carrying disease germs obviously improves, and has simplified step, has reduced cost.Other has the similar Salmonellas of a kind of principle to detect bag (sheet),, can directly detect Salmonellas at the body surface cleaning sampling, also can put into any quantitative sample liquid and cultivate the detection Salmonellas, its recall rate is higher.

As change corresponding substratum and then can detect other corresponding pathogenic bacteria.

Description of drawings

Fig. 1 has handle swab and band to fill in the detector tube structural representation for of the present invention.

Fig. 2 is have handle swab and a detector tube structural representation with cover of the present invention.

Fig. 3 is a kind of have handle swab and detection bag constructions synoptic diagram thereof of the present invention.

Fig. 4 is a kind of swab and detection bag constructions synoptic diagram thereof that contains substratum of the present invention.

Fig. 5 is a kind of detection slab construction synoptic diagram of the present invention.

Fig. 6 is a multi-functional detection bag constructions synoptic diagram of the present invention.

Fig. 7 makes the swab of handle and detects the bag constructions synoptic diagram with inner bag for of the present invention.

Embodiment

Swab shown in Figure 1 is made of shaft-like handle (1) and column swab head (4), and this kind swab both can use separately, can install test tube plug (9) again additional and be put in formation detector tube in the test tube (6) on its handle.Wherein a kind of swab, the long 6～20cm of handle (1), the long 1～10cm of hypomere swab head (4) contains solid phase and pours into enrichment medium and reagent, and the lower end is round and smooth.After adding water, but Direct Sampling gets final product observing response after the cultivation.The special-purpose anus of wherein a kind of pathogen enterobacteria primary dcreening operation swab and Salmonellas is wiped away substratum and the reagent that contains corresponding one to multiple kind biochemistry and immunological response respectively.

Detector tube shown in Figure 2, only the test tube plug (9) with Fig. 1 changes test tube lid (10), other same Fig. 1 into.This is second kind of embodiment of detector tube.

Swab shown in Figure 3 is made of flat handle (2) and sheet swab head (5).And be put in the plastics bag (7) detect bag.Because the handle utilization is arranged, be convenient to the hand-held body surface sampling that is used as, also can directly leach liquid sample.

Swab shown in Figure 4 is to pour into the sheet swab (5) of enrichment medium and be put in the plastics bag (7) and constitute containing solid phase.This is for detecting the another kind of embodiment of bag.Wherein a kind ofly be exclusively used in the swab that pathogen enterobacteria checks and constitute, and contain solid phase and pour into enrichment medium and reagent, can be applicable to the sample of people, animal, food and body surface bacterioscopy by the multilayer build-up water-absorbing material.

Detection shown in Figure 5 is dull and stereotyped, is made of sheet swab (5) and plate (8).Can be used for liquid sample and detect, also can be used as dry finished product substratum and use,, be the finished product substratum with before adding sterilized water.

Figure 6 shows that multi-functional detection bag.Place formation in the same plastics bag (7) by a plurality of swabs that detect different pathogenic bacterium, behind the adding sample, can detect multiple microorganism simultaneously.Method can be prepared into food poisoning detection, institute's sense monitoring, monitoring of infectious disease series detection bag according to this.After adding handle on the swab, operation can be more convenient.

Figure 7 shows that with the inner bag to be the detection bag of handle, be fixed on the inner bag (3), promptly constitute in the bag (7) outside being put in again by swab (5).Cai Yang Time, finger or other object can insert Direct Sampling operation in the inner bag, also can not pollute swab.

In a test tube, plate, also can put into a plurality of swabs to constitute multi-functional detection pipe, multifunctional examining master plate.

Solid phase is poured into the used swab of enrichment, handle, container, can require to be made into different shapes and specification according to the difference of various bacteriums and sample.On the swab used solid phase pour into enrichment medium by with detected corresponding substratum of microorganism and reagent, different microorganisms, different ingredients.As this kind substratum and reagent directly are put in the containers such as test tube, flat board, reagent bottle and plastics bag, also can be used as solid phase and pour into and increase bacterium.

Used swab is drawn materials extensively, and swab handle (1,2,3) can have certain degree of hardness and nontoxic material constitutes by plastics, bamboo, timber, paper, glass, metal etc.Swab (4,5) is made of the non-toxic material that cotton, paper, plastics, rubber, animal and plant fiber etc. have water-retaining capacity.Container (6,7,8) is made of nontoxic transparent materials such as plastics, transparent paper, glass.

More than all associated plants, the solid phase when all being made for various microorganism detection is poured into and is increased bacterium and use.

Claims

1. solid phase is poured into enrichment and the supporting device of using, it is characterized in that, it is to use solid phase to pour into enrichment medium and contain solid phase and pour into the device of enrichment medium and replace the liquid enrichment liquid that solid phase is poured into enrichment, by pouring into or the methods of the similar cultural method that pours into culture of isolated pathogenic bacterium from the primary sample that contains assorted bacterium.

2. pour into enrichment and the supporting device of using according to the described solid phase of claim 1, it is characterized in that, used substratum is that the corresponding solid phase of various bacteriums is poured into enrichment medium, the different bacterium difference of then filling a prescription, equipment therefor are to contain the solid phase that contains that the corresponding solid phase of various bacteriums pours into the container of enrichment medium (test tube, flat board, reagent bottle, plastics bag) and have an absorbent function to pour into the column of enrichment medium (4), sheet (5) swab.

3. pour into enrichment and the supporting device of using according to the described solid phase of claim 2, it is characterized in that, contain solid phase pour into the column of enrichment medium (4), sheet (5) swab can be respectively and the handle that is shaft-like (1), flat (2), bag shape (3), be combined into the handle swab, and can be respectively be combined into detector tube with corresponding container (6,7,8), detect bag, detect dull and stereotypedly, will possess multiple function after putting a plurality of swabs in the container.

4. pour into enrichment and the supporting device of using according to the described solid phase of claim 2, it is characterized in that, a kind ofly contain the special detection pipe that solid phase is poured into enrichment medium,, be put in the test tube (6) and constitute by column (4) swab of band handle (1), can connect test tube plug (9) or test tube lid (10) on the handle, a kind of special-purpose anal swab that solid phase is poured into enrichment medium, the long 6～20cm of handle (1), long 1～10cm of hypomere swab (4) of containing, terminal round and smooth, be put in formation dedicated test pipe in the test tube (6).

5. pour into enrichment and the supporting device of using according to the described solid phase of claim 2, it is characterized in that, a kind ofly contain the special detection bag that solid phase is poured into enrichment medium, its swab is sheet (5), can be formed by stacking by the multi-layer absorbent material, can connect the handle of shaft-like (1), flat (2), bag shape (3), place promptly to constitute special detection bag in the plastics bag (7).