CN102266583A - Preparation method of silver-loading modified bacterial cellulose based compound functional wet dressing - Google Patents
Preparation method of silver-loading modified bacterial cellulose based compound functional wet dressing Download PDFInfo
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Abstract
The invention relates to a preparation method of a silver-loading modified bacterial cellulose based compound functional wet dressing, comprising the following steps of: (1) putting lyophilized bacterial cellulose into a nitroxyl/enzyme system solution, adding an oxidizer for reaction; (2) adding into a diamine substance solution, heating for reaction; (3) then soaking into a silver nitrate solution to obtain bacterial cellulose selectively loading silver ions; or performing high pressure reduction on the bacterial cellulose to obtain bacterial cellulose selectively loading nano-silver particles; and (4) adding into a compound solution, standing at room temperature, then taking out, partially drying, packing, and sterilizing to obtain the silver-loading modified bacterial cellulose based compound functional wet dressing. By the method provided by the invention, antibiotic nano-silver can be loaded on the C6 hydroxyl of the bacterial cellulose, and a functional medicament can be compounded. The prepared dressing can be used for treating all kinds of skin injury, wound, surfaces of burn and scald wounds, and the like, and has good antibiotic and repairing effects.
Description
Technical field
The present invention relates to the preparation method of the composite function hygrometric state of a kind of silver-carrying modified Bacterial cellulose base dressing.
Background technology
Bacterial cellulose (Bacterial cellulose is called for short BC) is by the bacteriogenic fibrid element of part, in order to be different from the cellulose that plant produces, also claims micro organism cellulose.Bacterial cellulose is a kind of new material biology of function admirable, in recent years by people's broad research.Though Bacterial cellulose is formed similar with general cellulose, its physics, chemical property uniqueness.As: the nano level superfine network structure; Elastic modelling quantity, hot strength and wet strength are very high, and mechanical performance is better than the conventional fibre element; Good hydrophilic property because inside is full of " duct ", has well and breathes freely, and is permeable, water holding capacity; Have good biocompatibility, not only adaptability is good but also biodegradable; The physical and chemical performance of Bacterial cellulose can make by adopting different strains or cultural method to synthesize, and has excellent controllability; The raw material sources of synthetic Bacterial cellulose are abundant, low price, and this has also reduced the cost of Bacterial cellulose.
Based on above excellent properties, Bacterial cellulose can be used as a kind of novel " moist " dressing.This dressing has excellent physics, chemistry and engineering properties: ultra-fine network structure, high-tensile and elastic modelling quantity, high-hydrophilic, high ventilation performance, and biocompatibility and biodegradability are arranged.Because it has the structure of nano-scale, can consider some functional particles of load, perhaps group on Bacterial cellulose.Additional properties when the raising Bacterial cellulose uses as dressing.
Chinese patent CN101586309A utilizes the silver ion in the hydroxyl absorption silver ammino solution on the Bacterial cellulose, and reaction generates nano silver/silver chloride as Reducing agent with the chemical reagent that contains aldehyde again; Chinese patent CN101264335A utilizes hydroxyl on the Bacterial cellulose to adsorb silver ion in the silver nitrate solution, again with the chlorate solution reaction, and the compound generation nano silver/silver chloride of original position on bacteria cellulose film.But hydroxyl that above two kinds of methods all are dependence Bacterial cellulose surfaces to be rich in and silver ion form certain coordination compound and exist, this complexes system is an avtive spot with the coordination compound metal ion under the effect of Reducing agent, precipitant again, generates the nanoparticle of certain size.But nanoparticle that the mode that relies on hydroxyl and metal ion to form coordination compound makes and the active force man between the bacteria cellulose film a little less than, easily from the surperficial landing of film.If entering human body from its surperficial landing along wound surface, a large amount of nanometer silvers and silver salt cause the silver calmness that human body is worked the mischief.
In addition, above-mentioned dressing with Bacterial cellulose composite nano silver, silver chloride only plays antimicrobial single effect, complex process in the regeneration and the reparation of human skin, if dressing can activate epithelial hyperplasia, promote that normal granulation tissue generates, the formation of the normal collagen fiber of guiding, accelerated in wounds or wound surface normally heal and have reducing swelling and alleviating pain, suppress fibrocellular propagation, prevent hyperplasia and unordered diffusion, so this dressing just can suppress the formation of special-shaped cicatrix, can effectively promote wound healing, desalination wound cicatrix.
Summary of the invention
The present invention relates to the preparation method of the composite function hygrometric state of a kind of silver-carrying modified Bacterial cellulose base dressing.
The preparation method of the composite function hygrometric state of a kind of silver-carrying modified Bacterial cellulose base of the present invention dressing, be that silver and functional medicine are anchored on respectively on the C6 of Bacterial cellulose and C2, the C3 position hydroxyl, anti-microbial property and the dispersing swelling and dissipating binds that silver is excellent, change rotten evacuation of pus, restrain skin protection, analgesia is antipruritic, the antianaphylactic function of antiinflammatory is composite, obtains the dressing of Compositional type Bacterial cellulose hygrometric state.
The prepared dressing of preparation method of the composite function hygrometric state of a kind of silver-carrying modified Bacterial cellulose base of the present invention dressing; be by modified bacteria cellulose; antibacterial nano-silver or silver ion and functional medicine are formed; described modified bacteria cellulose dressing is that nitroxyl/enzyme cooxidation system is only carried out selective oxidation to the primary hydroxyl of Bacterial cellulose C6 position and become carboxyl; and become the amination Bacterial cellulose with two amine substance reactions such as ethylenediamines; described antibacterial nano-silver or silver ion are to be anchored on the modified bacteria cellulose after producing coordinate bond and ionic bond formation chelate with it by the amido on the C6 position, and described functional medicine is the C2 that is kept by on the modified bacteria cellulose; the N that is contained in C3 position hydroxyl and the functional medicine; O; form hydrogen bond action between the F atom and be adsorbed on the silver-carrying modified Bacterial cellulose.
The preparation method of the composite function hygrometric state of a kind of silver-carrying modified Bacterial cellulose base of the present invention dressing, its preparation comprises the steps:
(1) earlier Bacterial cellulose is placed in the refrigerator freezing 3～12 hours, again with Bacterial cellulose lyophilization 24～48 hours; Lyophilization normally places freezer dryer to handle Bacterial cellulose; Perhaps Bacterial cellulose was placed 6～24 hours in 20～120 ℃ of vacuum drying ovens;
(2) dried Bacterial cellulose is immersed in the aqueous solution that contains 0.01～1mol/L nitroxyl compound and 0.01～10mol/L enzyme, quality-the volumetric concentration of described Bacterial cellulose in described aqueous solution is 2～20g/L, and to adjust above-mentioned pH value of water solution with the HCl of 1～10mol/L or HNO3 solution be 2～6;
(3) oxidizing agent solution that progressively dripped 1～10mol/L in 1～10 hour makes reaction, 20～100 ℃ of system temperatures.Described being reflected under the magnetic agitation situation carried out, and rotating speed is generally at 50～500r/min.Dripping quantity is 1/10th of a described aqueous solution volume;
(4) will taking out through the Bacterial cellulose that the last step handles, wash 3～5 times with distilled water and ethanol respectively, is 6～7 until its pH, promptly obtains the Bacterial cellulose with hydroxyl oxidize carboxylic acid on the C6 position;
(5) Bacterial cellulose of above-mentioned selective oxidation adds in the two amine substance solutions, quality-the volumetric concentration of the Bacterial cellulose of selective oxidation in two amine substance solutions is 1～10g/ml, be heated to 80～120 ℃ of backflows, behind reaction 2～4h, take out Bacterial cellulose after being cooled to room temperature;
(6) with the Bacterial cellulose that obtains with distillation washing 3～5 times, to Bacterial cellulose pH be 6.8～7.0, promptly obtain the aminating Bacterial cellulose of selectivity;
(7) the aminating Bacterial cellulose of above-mentioned selectivity is immersed in 2～720min in the silver nitrate solution that silver ion molar percentage concentration is 0.001～1mol/L, make the argent ion fully be adsorbed onto in the nanoscale aperture of Bacterial cellulose and on the fento surface, and carry out coordination with amino and form chelate;
(8) above-mentioned Bacterial cellulose is taken out and distilled water wash 3～5 times, promptly obtain the Bacterial cellulose of selectivity load silver ion;
Perhaps, further,
(a) Bacterial cellulose with above-mentioned selectivity load silver ion is heated to 100～150 ℃ in high-pressure sterilizing pot, and is forced into 0.1～1MPa, leaves standstill to handle 5～30min;
(b) above-mentioned Bacterial cellulose is taken out and distilled water wash 3～5 times, be the particulate Bacterial cellulose of selectivity loading nano silvery;
(9) with the Bacterial cellulose of above-mentioned selectivity load silver ion or the particulate Bacterial cellulose of selectivity loading nano silvery as matrix, impregnated in mass fraction and be 1%～99% combination solution, room temperature left standstill 10～200 minutes;
(10) dressing after handling is taken out, dried to moisture content is 80～98wt%, packing, sterilization, can obtain the dressing of silver-carrying modified Bacterial cellulose base Compositional type function hygrometric state.
Wherein, described nitroxyl compound refers to the organic nitroxyl compound that lacks the a-hydrogen atom;
Described enzyme refers to peroxidase or polyhydric phenols oxidase;
Described oxidizing agent solution refers to hypochlorous acid solution, hypochlorite solutions, chlorous acid solution, inferior aluminate solution or hydrogenperoxide steam generator;
Described two amine substance solutions are ethylenediamine solution, propane diamine solution, 1,4-butanediamine solution, 1,5-pentanediamine solution, 1,6-hexamethylene diamine solution, p-phenylenediamine (PPD) solution, m-diaminobenzene. solution or o-phenylenediamine solution;
Described compound is one or more combination of Chinese medicine, quintessence oil or somatomedin.
Described compound has the effect of " dispersing swelling and dissipating binds, change rotten evacuation of pus, convergence skin protection, antipruritic, the antiinflammatory antiallergic of analgesia ", comprises one or more combination of Chinese medicine, quintessence oil or somatomedin.
As optimized technical scheme:
Described Bacterial cellulose is a kind of Bacterial cellulose that produces in acetobacter xylinum, product acetobacter, acetify bacillus, Acetobacter pasteurianus, glucose bacillus, Agrobacterium, root nodule bacteria, sarcina, Pseudomonas cepacia, Pseudomonas cocovenenans or the campylobacter jejuni.
The dressing of the composite function hygrometric state of aforesaid a kind of silver-carrying modified Bacterial cellulose base; the nitroxyl compound of the described a-of lacking hydrogen atom is 2; 2; 5,5-tetramethyl piperidine-N-oxygen base (PROXYL), 4-methyl-TEMPO, 4-hydroxyl-TEMPO, 4-acetamido-TEMPO, 4-acetate-TEMPO or 4-methanesulfonic acid base-TEMPO.
The dressing of the composite function hygrometric state of aforesaid a kind of silver-carrying modified Bacterial cellulose base, described peroxidase is horseradish peroxidase, bean shell peroxidase, myeloperoxidase (MPO), lactoperoxidase or terrible umbrella peroxidase.Peroxidase can comprise plant, antibacterial, thread and other funguses and yeast from any source, and these peroxide need hydrogen peroxide as electron acceptor.
The dressing of the composite function hygrometric state of aforesaid a kind of silver-carrying modified Bacterial cellulose base, described polyhydric phenols oxidase is tyrosine (single phenol mono-oxygenase), catechol-oxydase (bis-phenol oxidase) or laccase.The polyhydric phenols oxidase can obtain from fungus, plant or animal, needs oxygen as electron acceptor.
The dressing of the composite function hygrometric state of aforesaid a kind of silver-carrying modified Bacterial cellulose base, described Chinese medicine is Radix Arnebiae (Radix Lithospermi), Radix Notoginseng, Folium Artemisiae Argyi, the Radix Astragali, Radix Et Rhizoma Rhei, the Pseudobulbus Bletillae (Rhizoma Bletillae), Borneolum Syntheticum, Flos Carthami, the Radix Angelicae Dahuricae, Herba Agrimoniae, Rhizoma Coptidis, Cortex Phellodendri, the waist Huang, Flos Caryophylli, Venenum Bufonis, Moschus, Folium Hibisci Mutabilis, Camphora, Fructus Cnidii, Cortex Pseudolaricis, Calamina, Calomelas, hydrargrum oxydatum crudum, pearl sand, Borax, Realgar, different sand, Margarita, succinum, Os Draconis, Sanguis Draxonis, Alumen, Calx, Rhizoma Polygoni Cuspidati, Radix Sanguisorbae, Corium elephatis, Os Draconis, Concha Ostreae, Calamina, Halloysitum Rubrum, the Lithargyrum, Galla Chinensis, Endoconcha Sepiae, Talcum, Mel, Aloe or Herba Ajugae.
The dressing of the composite function hygrometric state of aforesaid a kind of silver-carrying modified Bacterial cellulose base, described quintessence oil is Herba Lysimachiae foenum-graeci quintessence oil, tea tree ethereal oil, Herba Salviae Japonicae essential oil, Flos Pelargonii quintessence oil, Folium Pini quintessence oil, Olibanum quintessence oil or Flos Matricariae chamomillae quintessence oil.
The dressing of the composite function hygrometric state of aforesaid a kind of silver-carrying modified Bacterial cellulose base, described somatomedin is transforming growth factor, basic fibroblast growth factor, nerve growth factor, human vascular endothelial growth factor, platelet derived growth factor or epithelical cell growth factor.
Beneficial effect
The present invention is compared with prior art:
(1) optionally modified bacteria cellulose guarantees the activity of C2, C3 position hydroxyl simultaneously, hydroxyl on the C6 position is converted into amino, make the amino on the C6 position preferentially adsorb metal ion with forms such as ionic bond and coordinate bonds, coordination takes place form stable chelate, be that the site is reduced into elemental metals with it again with this metal ion, give the anti-microbial property of dressing excellence; Because the growth of silver metal nano-particle can also have effectively been controlled in the selectivity modification, has limited particulate reunion.
When (2) the composite nano silver granule reaches antimicrobial effect on guaranteeing Bacterial cellulose C6 position, functional medicine is to be adsorbed on the silver-carrying modified Bacterial cellulose by forming hydrogen bond action between N, the O that is contained in the C2 that is kept on the modified bacteria cellulose, C3 position hydroxyl and the functional medicine, F atom, reach reducing swelling and alleviating pain, suppress fibrocellular propagation, prevent hyperplasia and unordered diffusion, thereby suppress the synergistic function of the formation of special-shaped cicatrix.
The specific embodiment
Below in conjunction with the specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
(1) will place freezer dryer by the Bacterial cellulose that acetobacter xylinum produces, lyophilization 24 hours;
(2) Bacterial cellulose after the lyophilization is immersed in and contains 0.01mol/L 2,2,5, in the aqueous solution of 5-tetramethyl piperidine-N-oxygen base and 0.01mol/L horseradish peroxidase, quality-the volumetric concentration of described Bacterial cellulose in described aqueous solution is 2g/L, and with the HNO of 1mol/L
3It is 2 that solution is adjusted above-mentioned pH value of water solution;
(3) the hypochlorous acid solution that progressively dripped 1mol/L in 1 hour makes reaction, and the dripping quantity by volume is 1/10th of a described aqueous solution volume, 100 ℃ of reaction temperatures;
(4) will take out through the Bacterial cellulose that the last step handles, with distillation washing 5 times, reuse ethanol is washed 5 times, is 7 until its pH value, promptly obtains the selective oxidation Bacterial cellulose;
(5) above-mentioned selective oxidation Bacterial cellulose is added in the ethylenediamine solution, the quality-volumetric concentration of the Bacterial cellulose of selective oxidation in ethylenediamine solution is 10g/ml, is heated to 120 ℃ of backflows, behind the reaction 4h, takes out Bacterial cellulose after being cooled to room temperature;
(6) with the Bacterial cellulose distilled water flushing that obtains, to Bacterial cellulose pH be 7.0, promptly obtain the aminating Bacterial cellulose of selectivity;
(7) the aminating Bacterial cellulose of above-mentioned selectivity is immersed in 720min in the silver nitrate solution that silver ion molar percentage concentration is 1mol/L, make the argent ion fully be adsorbed onto in the nanoscale aperture of Bacterial cellulose and on the fento surface, and carry out coordination with amino and form chelate;
(8) will state that Bacterial cellulose takes out and with distilled water wash 5 times, be the Bacterial cellulose of selectivity load silver ion;
(9) with the Bacterial cellulose of above-mentioned selectivity load silver ion as matrix, add mass fraction and be 99% Radix Arnebiae (Radix Lithospermi) solution, completely be benchmark to soak, room temperature left standstill 200 minutes;
(10) dressing after handling is taken out, dried to moisture content is 98wt%, packing, sterilization, can obtain the dressing of silver-carrying modified Bacterial cellulose base Compositional type function hygrometric state.
Embodiment 2
(1) will place freezer dryer, lyophilization 24 hours by the Bacterial cellulose that produces the acetobacter generation;
(2) Bacterial cellulose after the lyophilization is immersed in the aqueous solution that contains 0.1mol/L 4-methyl-TEMPO and 0.1mol/L bean shell peroxidase, quality-volumetric concentration the 5g/L of described Bacterial cellulose in described aqueous solution, and to adjust above-mentioned pH value of water solution with the HCl solution of 5mol/L be 3;
(3) hypochlorite solutions that progressively dripped 2mol/L in 2 hours makes reaction, and the dripping quantity by volume is 1/10th of a described aqueous solution volume, 20 ℃ of reaction temperatures;
(4) will take out through the Bacterial cellulose that the last step handles, with distillation washing 3 times, reuse ethanol is washed 3 times, is 6 until its pH value, promptly obtains the selective oxidation Bacterial cellulose;
(5) above-mentioned selective oxidation Bacterial cellulose is added in the propane diamine solution, the quality-volumetric concentration of the Bacterial cellulose of selective oxidation in propane diamine solution is 9g/ml, is heated to 80 ℃ of backflows, behind the reaction 2h, takes out Bacterial cellulose after being cooled to room temperature;
(6) with the Bacterial cellulose distilled water flushing that obtains, to Bacterial cellulose pH be 6.8, promptly obtain the aminating Bacterial cellulose of selectivity;
(7) the aminating Bacterial cellulose of above-mentioned selectivity is immersed in 2min in the silver nitrate solution that silver ion molar percentage concentration is 0.001mol/L, make the argent ion fully be adsorbed onto in the nanoscale aperture of Bacterial cellulose and on the fento surface, and carry out coordination with amino and form chelate;
(8) will state that Bacterial cellulose takes out and with distilled water wash 4 times, be the Bacterial cellulose of selectivity load silver ion;
(9) with the Bacterial cellulose of above-mentioned selectivity load silver ion as matrix, add mass fraction and be 1% Radix Notoginseng and Folium Artemisiae Argyi combination solution, completely be benchmark to soak, room temperature left standstill 10 minutes;
(10) dressing after handling is taken out, dried to moisture content is 80wt%, packing, sterilization, can obtain the dressing of silver-carrying modified Bacterial cellulose base Compositional type function hygrometric state.
Embodiment 3
(1) will place freezer dryer, lyophilization 24 hours by the Bacterial cellulose that the acetify bacillus produces;
(2) Bacterial cellulose after the lyophilization is immersed in the aqueous solution that contains 0.51mol/L 4-hydroxyl-TEMPO and 0.5mol/L myeloperoxidase (MPO), the quality-volumetric concentration 10g/L of described Bacterial cellulose in described aqueous solution, and with the HNO of 5mol/L
3It is 4 that solution is adjusted above-mentioned pH value of water solution, 90 ℃ of reaction temperatures;
(3) in 3 hours progressively the chlorous acid solution of Dropwise 5 mol/L make reaction, the dripping quantity by volume is 1/10th of a described aqueous solution volume;
(4) will take out through the Bacterial cellulose that the last step handles, with distillation washing 5 times, reuse ethanol is washed 3 times, is 6 until its pH value, promptly obtains the selective oxidation Bacterial cellulose;
(5) above-mentioned selective oxidation Bacterial cellulose is added 1, in the 4-butanediamine solution, the Bacterial cellulose of selective oxidation is 1, quality-volumetric concentration in the 4-butanediamine solution is 9g/ml, be heated to 100 ℃ of backflows, behind the reaction 3h, take out Bacterial cellulose after being cooled to room temperature;
(6) with the Bacterial cellulose distilled water flushing that obtains, to Bacterial cellulose pH be 6.9, promptly obtain the aminating Bacterial cellulose of selectivity;
(7) the aminating Bacterial cellulose of above-mentioned selectivity is immersed in 400min in the silver nitrate solution that silver ion molar percentage concentration is 0.01mol/L, make the argent ion fully be adsorbed onto in the nanoscale aperture of Bacterial cellulose and on the fento surface, and carry out coordination with amino and form chelate;
(8) will state that Bacterial cellulose takes out and with distilled water wash 4 times, be the Bacterial cellulose of selectivity load silver ion;
(9) Bacterial cellulose with above-mentioned selectivity carrying silver ion is heated to 150 ℃ in high-pressure sterilizing pot, and pressurization 1MPa, leaves standstill and handles 30min;
(10) will state that Bacterial cellulose takes out and, be the particulate Bacterial cellulose of selectivity loading nano silvery with distilled water wash 5 times;
(11) with the particulate Bacterial cellulose of above-mentioned selectivity loading nano silvery as matrix, add mass fraction and be 49% the Radix Astragali, Radix Et Rhizoma Rhei and Pseudobulbus Bletillae (Rhizoma Bletillae) combination solution, completely be benchmark to soak, room temperature left standstill 100 minutes;
(12) dressing after handling is taken out, dried to moisture content is 85wt%, packing, sterilization, can obtain the dressing of silver-carrying modified Bacterial cellulose base Compositional type function hygrometric state.
Embodiment 4
(1) will place freezer dryer by the Bacterial cellulose that Acetobacter pasteurianus produces, lyophilization 24 hours;
(2) Bacterial cellulose after the lyophilization is immersed in the aqueous solution that contains 0.2mol/L 4-acetamido-TEMPO and 0.2mol/L lactoperoxidase, quality-volumetric concentration the 6g/L of described Bacterial cellulose in described aqueous solution, and to adjust above-mentioned pH value of water solution with the HCl solution of 6mol/L be 6;
(3) the inferior aluminate solution that progressively dripped 6mol/L in 6 hours makes reaction, and the dripping quantity by volume is 1/10th of a described aqueous solution volume, 80 ℃ of reaction temperatures;
(4) will take out through the Bacterial cellulose that the last step handles, with distillation washing 3 times, reuse ethanol is washed 3 times, is 6 until its pH value, promptly obtains the selective oxidation Bacterial cellulose.
(5) above-mentioned selective oxidation Bacterial cellulose is added 1, in the 5-pentanediamine solution, the Bacterial cellulose of selective oxidation is 1, quality-volumetric concentration in the 5-pentanediamine solution is 8g/ml, be heated to 90 ℃ of backflows, behind the reaction 3h, take out Bacterial cellulose after being cooled to room temperature;
(6) with the Bacterial cellulose distilled water flushing that obtains, to Bacterial cellulose pH be 7.0, promptly obtain the aminating Bacterial cellulose of selectivity;
(7) the aminating Bacterial cellulose of above-mentioned selectivity is immersed in 100min in the silver nitrate solution that silver ion molar percentage concentration is 0.1mol/L, make the argent ion fully be adsorbed onto in the nanoscale aperture of Bacterial cellulose and on the fento surface, and carry out coordination with amino and form chelate;
(9) Bacterial cellulose with above-mentioned selectivity carrying silver ion is heated to 100 ℃ in high-pressure sterilizing pot, and pressurization 0.1MPa, leaves standstill and handles 5min;
(10) will state that Bacterial cellulose takes out and, be the particulate Bacterial cellulose of selectivity loading nano silvery with distilled water wash 4 times;
(11) with the particulate Bacterial cellulose of above-mentioned selectivity loading nano silvery as matrix, add mass fraction and be 20% Borneolum Syntheticum, Flos Carthami, the Radix Angelicae Dahuricae and Herba Agrimoniae combination solution, completely be benchmark to soak, room temperature left standstill 150 minutes;
(12) dressing after handling is taken out, dried to moisture content is 85wt%, packing, sterilization, can obtain the dressing of silver-carrying modified Bacterial cellulose base Compositional type function hygrometric state.
Embodiment 5
(1) will place freezer dryer, lyophilization 24 hours by the Bacterial cellulose that the glucose bacillus produces;
(2) Bacterial cellulose after the lyophilization is immersed in the aqueous solution that contains 0.05mol/L 4-acetate-TEMPO and 0.05mol/L ghost umbrella peroxidase, the quality-volumetric concentration 5g/L of described Bacterial cellulose in described aqueous solution, and with the HNO of 5mol/L
3It is 6 that solution is adjusted above-mentioned pH value of water solution.
(3) in 5 hours progressively the hydrogenperoxide steam generator of Dropwise 5 mol/L make reaction, the dripping quantity by volume is 1/10th of a described aqueous solution volume, 30 ℃ of reaction temperatures;
(4) will take out through the Bacterial cellulose that the last step handles, with distillation washing 5 times, reuse ethanol is washed 5 times, is 7 until its pH value, promptly obtains the selective oxidation Bacterial cellulose.
(5) above-mentioned selective oxidation Bacterial cellulose is added 1, in the 6-hexamethylene diamine solution, the Bacterial cellulose of selective oxidation is 1, quality-volumetric concentration in the 6-hexamethylene diamine solution is 7g/ml, be heated to 90 ℃ of backflows, behind the reaction 4h, take out Bacterial cellulose after being cooled to room temperature;
(6) with the Bacterial cellulose distilled water flushing that obtains, to Bacterial cellulose pH be 6.8, promptly obtain the aminating Bacterial cellulose of selectivity;
(7) the aminating Bacterial cellulose of above-mentioned selectivity is immersed in 500min in the silver nitrate solution that silver ion molar percentage concentration is 0.5mol/L, make the argent ion fully be adsorbed onto in the nanoscale aperture of Bacterial cellulose and on the fento surface, and carry out coordination with amino and form chelate;
(8) will state that Bacterial cellulose takes out and with distilled water wash 4 times, be the Bacterial cellulose of selectivity load silver ion;
(9) Bacterial cellulose with above-mentioned selectivity carrying silver ion is heated to 130 ℃ in high-pressure sterilizing pot, and pressurization 0.4MPa, leaves standstill and handles 20min;
(10) will state that Bacterial cellulose takes out and, be the particulate Bacterial cellulose of selectivity loading nano silvery with distilled water wash 5 times;
(11) with the particulate Bacterial cellulose of above-mentioned selectivity loading nano silvery as matrix, impregnated in mass fraction and be 59% Rhizoma Coptidis, Cortex Phellodendri, waist Huang, Flos Caryophylli and Venenum Bufonis combination solution, room temperature left standstill 90 minutes;
(12) dressing after handling is taken out, dried to moisture content is 90wt%, packing, sterilization, can obtain the dressing of silver-carrying modified Bacterial cellulose base Compositional type function hygrometric state.
Embodiment 6
(1) will place freezer dryer by the Bacterial cellulose that Agrobacterium produces, lyophilization 24 hours;
(2) Bacterial cellulose after the lyophilization is immersed in the aqueous solution that contains 1mol/L 4-methanesulfonic acid base-TEMPO and 10mol/L tyrosine (single phenol mono-oxygenase), quality-volumetric concentration the 20g/L of described Bacterial cellulose in described aqueous solution, and to adjust above-mentioned pH value of water solution with the HCl solution of 10mol/L be 6;
(3) the hypochlorous acid solution that progressively dripped 10mol/L in 10 hours makes reaction, and the dripping quantity by volume is 1/10th of a described aqueous solution volume, 40 ℃ of reaction temperatures;
(4) will take out through the Bacterial cellulose that the last step handles, with distillation washing 4 times, reuse ethanol is washed 4 times, is 6 until its pH value, promptly obtains the selective oxidation Bacterial cellulose.
(5) above-mentioned selective oxidation Bacterial cellulose is added 1, in the 6-hexamethylene diamine solution, the Bacterial cellulose of selective oxidation is 1, quality-volumetric concentration in the 6-hexamethylene diamine solution is 6g/ml, be heated to 80 ℃ of backflows, behind the reaction 3h, take out Bacterial cellulose after being cooled to room temperature;
(6) with the Bacterial cellulose distilled water flushing that obtains, to Bacterial cellulose pH be 6.8, promptly obtain the aminating Bacterial cellulose of selectivity;
(7) the aminating Bacterial cellulose of above-mentioned selectivity is immersed in 600min in the silver nitrate solution that silver ion molar percentage concentration is 0.6mol/L, make the argent ion fully be adsorbed onto in the nanoscale aperture of Bacterial cellulose and on the fento surface, and carry out coordination with amino and form chelate;
(8) will state that Bacterial cellulose takes out and with distilled water wash 3 times, be the Bacterial cellulose of selectivity load silver ion;
(9) Bacterial cellulose with above-mentioned selectivity carrying silver ion is heated to 140 ℃ in high-pressure sterilizing pot, and pressurization 0.6MPa, leaves standstill and handles 25min;
(10) will state that Bacterial cellulose takes out and, be the particulate Bacterial cellulose of selectivity loading nano silvery with distilled water wash 3 times;
(11) with the particulate Bacterial cellulose of above-mentioned selectivity loading nano silvery as matrix, impregnated in mass fraction and be 39% Moschus, Folium Hibisci Mutabilis, Camphora, Fructus Cnidii, Cortex Pseudolaricis and Calamina combination solution, room temperature left standstill 60 minutes;
(12) dressing after handling is taken out, dried to moisture content is 95wt%, packing, sterilization, can obtain the dressing of silver-carrying modified Bacterial cellulose base Compositional type function hygrometric state.
Embodiment 7
(1) will place freezer dryer by the Bacterial cellulose that root nodule bacteria produce, lyophilization 24 hours;
(2) Bacterial cellulose after the lyophilization is immersed in and contains 0.75mol/L 2,2,5, in the aqueous solution of 5-tetramethyl piperidine-N-oxygen base and 7.5mol/L catechol-oxydase (bis-phenol oxidase), quality-volumetric concentration the 15g/L of described Bacterial cellulose in described aqueous solution, and to adjust above-mentioned pH value of water solution with the HCl solution of 7.5mol/L be 4; .
(3) hypochlorite solutions that progressively dripped 8mol/L in 8 hours makes reaction, and the dripping quantity by volume is 1/10th of a described aqueous solution volume, 50 ℃ of reaction temperatures;
(4) will take out through the Bacterial cellulose that the last step handles, with distillation washing 3 times, reuse ethanol is washed 4 times, is 7 until its pH value, promptly obtains the selective oxidation Bacterial cellulose.
(5) above-mentioned selective oxidation Bacterial cellulose is added in the p-phenylenediamine (PPD) solution, quality-the volumetric concentration of the Bacterial cellulose of selective oxidation in p-phenylenediamine (PPD) solution is 5g/ml, be heated to 100 ℃ of backflows, behind the reaction 3h, take out Bacterial cellulose after being cooled to room temperature;
(6) with the Bacterial cellulose distilled water flushing that obtains, to Bacterial cellulose pH be 6.8, promptly obtain the aminating Bacterial cellulose of selectivity;
(7) the aminating Bacterial cellulose of above-mentioned selectivity is immersed in 700min in the silver nitrate solution that silver ion molar percentage concentration is 0.7mol/L, make the argent ion fully be adsorbed onto in the nanoscale aperture of Bacterial cellulose and on the fento surface, and carry out coordination with amino and form chelate;
(8) will state that Bacterial cellulose takes out and with distilled water wash 5 times, be the Bacterial cellulose of selectivity load silver ion;
(9) Bacterial cellulose with above-mentioned selectivity carrying silver ion is heated to 110 ℃ in high-pressure sterilizing pot, and pressurization 0.7MPa, leaves standstill and handles 20min;
(10) will state that Bacterial cellulose takes out and, be the particulate Bacterial cellulose of selectivity loading nano silvery with distilled water wash 5 times;
(11) with the particulate Bacterial cellulose of above-mentioned selectivity loading nano silvery as matrix, impregnated in mass fraction and be 69% Calomelas, hydrargrum oxydatum crudum, pearl sand, Borax, Realgar, different sand and Margarita combination solution, room temperature left standstill 100 minutes;
(12) dressing after handling is taken out, dried to moisture content is 85wt%, packing, sterilization, can obtain the dressing of silver-carrying modified Bacterial cellulose base Compositional type function hygrometric state.
Embodiment 8
(1) will place freezer dryer by the Bacterial cellulose that sarcina produces, lyophilization 24 hours;
(2) Bacterial cellulose after the lyophilization is immersed in the aqueous solution that contains 0.5mol/L 4-acetate-TEMPO and 6mol/L laccase, the quality-volumetric concentration 15g/L of described Bacterial cellulose in described aqueous solution, and with the HNO of 5mol/L
3It is 6 that solution is adjusted above-mentioned pH value of water solution;
(3) in 10 hours progressively the chlorous acid solution of Dropwise 5 mol/L make reaction, the dripping quantity by volume is 1/10th of a described aqueous solution volume, 60 ℃ of reaction temperatures;
(4) will take out through the Bacterial cellulose that the last step handles, with distillation washing 5 times, reuse ethanol is washed 4 times, is 6 until its pH value, promptly obtains the selective oxidation Bacterial cellulose.
(5) above-mentioned selective oxidation Bacterial cellulose is added in the m-diaminobenzene. solution, quality-the volumetric concentration of the Bacterial cellulose of selective oxidation in m-diaminobenzene. solution is 4g/ml, be heated to 110 ℃ of backflows, behind the reaction 4h, take out Bacterial cellulose after being cooled to room temperature;
(6) with the Bacterial cellulose distilled water flushing that obtains, to Bacterial cellulose pH be 7.0, promptly obtain the aminating Bacterial cellulose of selectivity;
(7) the aminating Bacterial cellulose of above-mentioned selectivity is immersed in 100min in the silver nitrate solution that silver ion molar percentage concentration is 0.05mol/L, make the argent ion fully be adsorbed onto in the nanoscale aperture of Bacterial cellulose and on the fento surface, and carry out coordination with amino and form chelate;
(8) will state that Bacterial cellulose takes out and with distilled water wash 5 times, be the Bacterial cellulose of selectivity load silver ion;
(9) Bacterial cellulose with above-mentioned selectivity carrying silver ion is heated to 140 ℃ in high-pressure sterilizing pot, and pressurization 0.8MPa, leaves standstill and handles 25min;
(10) will state that Bacterial cellulose takes out and, be the particulate Bacterial cellulose of selectivity loading nano silvery with distilled water wash 4 times;
(11) with the particulate Bacterial cellulose of above-mentioned selectivity loading nano silvery as matrix, impregnated in mass fraction and be 60% succinum, Os Draconis, Sanguis Draxonis, Alumen, Calx, Rhizoma Polygoni Cuspidati, Radix Sanguisorbae and Corium elephatis combination solution, room temperature left standstill 50 minutes;
(12) dressing after handling is taken out, dried to moisture content is 85wt%, packing, sterilization, can obtain the dressing of silver-carrying modified Bacterial cellulose base Compositional type function hygrometric state.
Embodiment 9
(1) will place freezer dryer by the Bacterial cellulose that Pseudomonas cepacia produces, lyophilization 24 hours;
(2) Bacterial cellulose after the lyophilization is immersed in the aqueous solution that contains 1mol/L 4-methanesulfonic acid base-TEMPO and 10mol/L tyrosine (single phenol mono-oxygenase), quality-volumetric concentration the 20g/L of described Bacterial cellulose in described aqueous solution, and to adjust above-mentioned pH value of water solution with the HCl solution of 10mol/L be 6;
(3) the hypochlorous acid solution that progressively dripped 10mol/L in 10 hours makes reaction, and the dripping quantity by volume is 1/10th of a described aqueous solution volume, 40 ℃ of reaction temperatures;
(4) will take out through the Bacterial cellulose that the last step handles, with distillation washing 5 times, reuse ethanol is washed 5 times, is 6 until its pH value, promptly obtains the selective oxidation Bacterial cellulose.
(5) above-mentioned selective oxidation Bacterial cellulose is added in the o-phenylenediamine solution, quality-the volumetric concentration of the Bacterial cellulose of selective oxidation in o-phenylenediamine solution is 3g/ml, be heated to 120 ℃ of backflows, behind the reaction 4h, take out Bacterial cellulose after being cooled to room temperature;
(6) with the Bacterial cellulose distilled water flushing that obtains, to Bacterial cellulose pH be 6.8, promptly obtain the aminating Bacterial cellulose of selectivity;
(7) the aminating Bacterial cellulose of above-mentioned selectivity is immersed in 700min in the silver nitrate solution that silver ion molar percentage concentration is 0.9mol/L, make the argent ion fully be adsorbed onto in the nanoscale aperture of Bacterial cellulose and on the fento surface, and carry out coordination with amino and form chelate;
(8) will state that Bacterial cellulose takes out and with distilled water wash 5 times, be the Bacterial cellulose of selectivity load silver ion;
(9) Bacterial cellulose with above-mentioned selectivity carrying silver ion is heated to 145 ℃ in high-pressure sterilizing pot, and pressurization 0.9MPa, leaves standstill and handles 15min;
(10) will state that Bacterial cellulose takes out and, be the particulate Bacterial cellulose of selectivity loading nano silvery with distilled water wash 5 times;
(11) with the particulate Bacterial cellulose of above-mentioned selectivity loading nano silvery as matrix, impregnated in mass fraction and be 70% Os Draconis, Concha Ostreae, Calamina, Halloysitum Rubrum, Lithargyrum, Galla Chinensis, Endoconcha Sepiae, Talcum and Mel combination solution, room temperature left standstill 200 minutes;
(12) dressing after handling is taken out, dried to moisture content is 98wt%, packing, sterilization, can obtain the dressing of silver-carrying modified Bacterial cellulose base Compositional type function hygrometric state.
Embodiment 10
(1) will place freezer dryer by the Bacterial cellulose that Pseudomonas cocovenenans produces, lyophilization 24 hours;
(2) Bacterial cellulose after the lyophilization is immersed in and contains 0.75mol/L 2,2,5, in the aqueous solution of 5-tetramethyl piperidine-N-oxygen base and 7.5mol/L catechol-oxydase (bis-phenol oxidase), quality-volumetric concentration the 15g/L of described Bacterial cellulose in described aqueous solution, and to adjust above-mentioned pH value of water solution with the HCl solution of 7.5mol/L be 4; .
(3) hypochlorite solutions that progressively dripped 8mol/L in 8 hours makes reaction, and the dripping quantity by volume is 1/10th of a described aqueous solution volume, 50 ℃ of reaction temperatures;
(4) will take out through the Bacterial cellulose that the last step handles, with distillation washing 5 times, reuse ethanol is washed 3 times, is 7 until its pH value, promptly obtains the selective oxidation Bacterial cellulose;
(5) above-mentioned selective oxidation Bacterial cellulose is added in the ethylenediamine solution, the quality-volumetric concentration of the Bacterial cellulose of selective oxidation in ethylenediamine solution is 2g/ml, is heated to 80 ℃ of backflows, after the reaction 2, takes out Bacterial cellulose after being cooled to room temperature;
(6) with the Bacterial cellulose distilled water flushing that obtains, to Bacterial cellulose pH be 6.8, promptly obtain the aminating Bacterial cellulose of selectivity;
(7) the aminating Bacterial cellulose of above-mentioned selectivity is immersed in 400min in the silver nitrate solution that silver ion molar percentage concentration is 0.4mol/L, make the argent ion fully be adsorbed onto in the nanoscale aperture of Bacterial cellulose and on the fento surface, and carry out coordination with amino and form chelate;
(8) will state that Bacterial cellulose takes out and with distilled water wash 4 times, be the Bacterial cellulose of selectivity load silver ion;
(9) Bacterial cellulose with above-mentioned selectivity carrying silver ion is heated to 140 ℃ in high-pressure sterilizing pot, and pressurization 0.4MPa, leaves standstill and handles 24min;
(10) will state that Bacterial cellulose takes out and, be the particulate Bacterial cellulose of selectivity loading nano silvery with distilled water wash 3 times;
(11) with the particulate Bacterial cellulose of above-mentioned selectivity loading nano silvery as matrix, impregnated in mass fraction and be 80% Aloe, Herba Ajugae, Herba Lysimachiae foenum-graeci quintessence oil, tea tree ethereal oil, Herba Salviae Japonicae essential oil, Flos Pelargonii quintessence oil, Folium Pini quintessence oil, Olibanum quintessence oil, Flos Matricariae chamomillae quintessence oil and transforming growth factor combination solution, room temperature left standstill 120 minutes;
(12) dressing after handling is taken out, dried to moisture content is 80wt%, packing, sterilization, can obtain the dressing of silver-carrying modified Bacterial cellulose base Compositional type function hygrometric state.
Embodiment 11
(1) will place freezer dryer by the Bacterial cellulose that campylobacter jejuni produces, lyophilization 24 hours;
(2) Bacterial cellulose after the lyophilization is immersed in the aqueous solution that contains 0.5mol/L 4-acetate-TEMPO and 6mol/L laccase, the quality-volumetric concentration 15g/L of described Bacterial cellulose in described aqueous solution, and with the HNO of 5mol/L
3It is 6 that solution is adjusted above-mentioned pH value of water solution;
(3) in 10 hours progressively the chlorous acid solution of Dropwise 5 mol/L make reaction, the dripping quantity by volume is 1/10th of a described aqueous solution volume, 60 ℃ of reaction temperatures;
(4) will take out through the Bacterial cellulose that the last step handles, with distillation washing 3 times, reuse ethanol is washed 3 times, is 6 until its pH value, promptly obtains the selective oxidation Bacterial cellulose.
(5) above-mentioned selective oxidation Bacterial cellulose is added in the propane diamine solution, the quality-volumetric concentration of the Bacterial cellulose of selective oxidation in propane diamine solution is 1g/ml, is heated to 100 ℃ of backflows, behind the reaction 3h, takes out Bacterial cellulose after being cooled to room temperature;
(6) with the Bacterial cellulose distilled water flushing that obtains, to Bacterial cellulose pH be 6.9, promptly obtain the aminating Bacterial cellulose of selectivity;
(7) the aminating Bacterial cellulose of above-mentioned selectivity is immersed in 20min in the silver nitrate solution that silver ion molar percentage concentration is 0.05mol/L, make the argent ion fully be adsorbed onto in the nanoscale aperture of Bacterial cellulose and on the fento surface, and carry out coordination with amino and form chelate;
(8) will state that Bacterial cellulose takes out and with distilled water wash 4 times, be the Bacterial cellulose of selectivity load silver ion;
(9) Bacterial cellulose with above-mentioned selectivity carrying silver ion is heated to 100 ℃ in high-pressure sterilizing pot, and pressurization 1MPa, leaves standstill and handles 5min;
(10) will state that Bacterial cellulose takes out and, be the particulate Bacterial cellulose of selectivity loading nano silvery with distilled water wash 4 times;
(11) with the particulate Bacterial cellulose of above-mentioned selectivity loading nano silvery as matrix, impregnated in mass fraction and be 90% basic fibroblast growth factor, nerve growth factor, human vascular endothelial growth factor, platelet derived growth factor and epithelical cell growth factor combination solution, room temperature left standstill 100 minutes;
(12) dressing after handling is taken out, dried to moisture content is 80wt%, packing, sterilization, can obtain the dressing of silver-carrying modified Bacterial cellulose base Compositional type function hygrometric state.
Claims (8)
1. the preparation method of the composite function hygrometric state of silver-carrying modified Bacterial cellulose base dressing is characterized in that comprising the steps:
(1) Bacterial cellulose is placed freezer dryer lyophilization 24～48 hours;
(2) Bacterial cellulose after the lyophilization is immersed in the aqueous solution that contains 0.01～1mol/L nitroxyl compound and 0.01～10mol/L enzyme; quality-the volumetric concentration of described Bacterial cellulose in described aqueous solution is 2～20g/L, and with HCl or the HNO of 1～10mol/L
3It is 2～6 that solution is adjusted above-mentioned pH value of water solution;
(3) oxidizing agent solution that progressively dripped 1～10mol/L in 1～10 hour makes reaction, and the dripping quantity by volume is 1/10th of a described aqueous solution volume;
(4) will take out through the Bacterial cellulose that the last step handles, distillation washing 3～5 times, reuse ethanol 3～5 times is 6～7 until its pH value, obtains the Bacterial cellulose of selective oxidation;
(5) Bacterial cellulose with above-mentioned selective oxidation adds in the two amine substance solutions, quality-the volumetric concentration of the Bacterial cellulose of described selective oxidation in described two amine substance solutions is 1～10g/ml, be heated to 80～120 ℃ of backflows, behind reaction 2～4h, take out Bacterial cellulose after being cooled to room temperature;
(6) with the Bacterial cellulose that obtains with distillation washing 3～5 times, to Bacterial cellulose pH be about 6.8～7.0, promptly obtain the aminating Bacterial cellulose of selectivity;
(7) the aminating Bacterial cellulose of above-mentioned selectivity is immersed in 2～720min in the silver nitrate solution that silver ion molar percentage concentration is 0.001～1mol/L, make silver ion fully be adsorbed onto in the nanoscale aperture of Bacterial cellulose and on the fento surface, and carry out coordination with amino and form chelate;
(8) above-mentioned Bacterial cellulose is taken out and distilled water wash 3～5 times, be the Bacterial cellulose of selectivity load silver ion; Perhaps, further,
(a) Bacterial cellulose with above-mentioned selectivity load silver ion is heated to 100～150 ℃ in high-pressure sterilizing pot, and is forced into 0.1～1MPa, leaves standstill to handle 5～30min;
(b) above-mentioned Bacterial cellulose is taken out and distilled water wash 3～5 times, be the particulate Bacterial cellulose of selectivity loading nano silvery;
(9) with the Bacterial cellulose of above-mentioned selectivity load silver ion or the particulate Bacterial cellulose of selectivity loading nano silvery as matrix, impregnated in mass fraction and be 1%～99% combination solution, room temperature left standstill 10～200 minutes;
(10) dressing after handling is taken out, dried to moisture content is 80～98wt%, packing, sterilization, can obtain the dressing of silver-carrying modified Bacterial cellulose base Compositional type function hygrometric state;
Wherein,
Described nitroxyl compound refers to the organic nitroxyl compound that lacks the a-hydrogen atom;
Described enzyme refers to peroxidase or polyhydric phenols oxidase;
Described oxidizing agent solution refers to hypochlorous acid solution, hypochlorite solutions, chlorous acid solution, inferior aluminate solution or hydrogenperoxide steam generator;
Described two amine substance solutions are ethylenediamine solution, propane diamine solution, 1,4-butanediamine solution, 1,5-pentanediamine solution, 1,6-hexamethylene diamine solution, p-phenylenediamine (PPD) solution, m-diaminobenzene. solution or o-phenylenediamine solution;
Described compound is one or more combination of Chinese medicine, quintessence oil or somatomedin.
2. the preparation method of the composite function hygrometric state of a kind of silver-carrying modified Bacterial cellulose base according to claim 1 dressing, it is characterized in that described Bacterial cellulose is a kind of Bacterial cellulose that produces in acetobacter xylinum, product acetobacter, acetify bacillus, Acetobacter pasteurianus, glucose bacillus, Agrobacterium, root nodule bacteria, sarcina, Pseudomonas cepacia, Pseudomonas cocovenenans or the campylobacter jejuni.
3. the preparation method of the composite function hygrometric state of a kind of silver-carrying modified Bacterial cellulose base according to claim 1 dressing; it is characterized in that; the nitroxyl compound of the described a-of lacking hydrogen atom is 2; 2; 5,5-tetramethyl piperidine-N-oxygen base, 4-methyl-TEMPO, 4-hydroxyl-TEMPO, 4-acetamido-TEMPO, 4-acetate-TEMPO or 4-methanesulfonic acid base-TEMPO.
4. the preparation method of the composite function hygrometric state of a kind of silver-carrying modified Bacterial cellulose base according to claim 1 dressing, it is characterized in that described peroxidase is horseradish peroxidase, bean shell peroxidase, myeloperoxidase (MPO), lactoperoxidase or terrible umbrella peroxidase.
5. the preparation method of the composite function hygrometric state of a kind of silver-carrying modified Bacterial cellulose base according to claim 1 dressing is characterized in that described polyhydric phenols oxidase is tyrosine, catechol-oxydase or laccase.
6. the preparation method of the composite function hygrometric state of a kind of silver-carrying modified Bacterial cellulose base according to claim 1 dressing, it is characterized in that described Chinese medicine is Radix Arnebiae (Radix Lithospermi), Radix Notoginseng, Folium Artemisiae Argyi, the Radix Astragali, Radix Et Rhizoma Rhei, the Pseudobulbus Bletillae (Rhizoma Bletillae), Borneolum Syntheticum, Flos Carthami, the Radix Angelicae Dahuricae, Herba Agrimoniae, Rhizoma Coptidis, Cortex Phellodendri, the waist Huang, Flos Caryophylli, Venenum Bufonis, Moschus, Folium Hibisci Mutabilis, Camphora, Fructus Cnidii, Cortex Pseudolaricis, Calamina, Calomelas, hydrargrum oxydatum crudum, pearl sand, Borax, Realgar, different sand, Margarita, succinum, Os Draconis, Sanguis Draxonis, Alumen, Calx, Rhizoma Polygoni Cuspidati, Radix Sanguisorbae, Corium elephatis, Os Draconis, Concha Ostreae, Calamina, Halloysitum Rubrum, the Lithargyrum, Galla Chinensis, Endoconcha Sepiae, Talcum, Mel, Aloe or Herba Ajugae.
7. the preparation method of the composite function hygrometric state of a kind of silver-carrying modified Bacterial cellulose base according to claim 1 dressing, it is characterized in that described quintessence oil is Herba Lysimachiae foenum-graeci quintessence oil, tea tree ethereal oil, Herba Salviae Japonicae essential oil, Flos Pelargonii quintessence oil, Folium Pini quintessence oil, Olibanum quintessence oil or Flos Matricariae chamomillae quintessence oil.
8. the preparation method of the composite function hygrometric state of a kind of silver-carrying modified Bacterial cellulose base according to claim 1 dressing, it is characterized in that described somatomedin is transforming growth factor, basic fibroblast growth factor, nerve growth factor, human vascular endothelial growth factor, platelet derived growth factor or epithelical cell growth factor.
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Application publication date: 20111207 |