CN102262059B - Automatic analysis meter and methods for reducing sugar content and water-soluble total sugar content - Google Patents
Automatic analysis meter and methods for reducing sugar content and water-soluble total sugar content Download PDFInfo
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Abstract
The invention relates to an automatic analysis meter and methods for measuring the reducing sugar content and the water-soluble total sugar content. The automatic analysis meter comprises a sample injection device and a color development detection device, wherein the sample injection device comprises an automatic sample injector and a first peristaltic pump; the first peristaltic pump is provided with a first pump pipe; a sample injection needle of the automatic sample injector is connected with a through opening F of a multi-way valve through the first pump pipe; a through opening A of the multi-way valve is connected with a waste liquid trough; a first back pressure adjuster is arranged between the through opening A of the multi-way valve and the waste liquid trough; the color development detection device comprises a second peristaltic pump provided with a second pump pipe, a third pump pipe, a fourth pump pipe, a fifth pump pipe, a sixth pump pipe and a seventh pump pipe; both ends of a sampling ring are connected with a through opening B and a through opening E of the multi-way valve; a through opening D of the multi-way valve is connected with flow carrying solution through the second pump pipe; and a through opening C of the multi-way valve is connected with a first tee joint, an online heating digestion module, a second tee joint, a first knotted reactor, a third tee joint, a fourth tee joint, an online heating color development module, a fifth tee joint, a second knotted reactor, a flow cell, a detector, a data connection cable and a computer processing system respectively. The automatic analysis meter can automatically and continuously test the sample accurately and quickly.
Description
Technical field
The present invention relates to a kind of full-automatic flow injection analyzer, relate in particular to automatic analyzer and the analytical approach thereof of a kind of reducing sugar and Water-soluble Total Sugar content.
Background technology
At present, the mensuration of the reducing sugar in the materials such as food, plant and Water-soluble Total Sugar content, many dependence manual operationss, its complex steps, personal error is larger, and precision is poor, and test speed is slow, and toxic reagent may cause damage to operating personnel.Use at present high performance liquid chromatograph can measure reducing sugar, but cannot directly measure Water-soluble Total Sugar content, and instrument is expensive, test speed is slow.At present, on market, there is commercial bubble interval Continuous Flow Analysis instrument, can directly measure the content of reducing sugar and Water-soluble Total Sugar, but need to there is extra bubble adding apparatus, apparatus structure is complicated, expensive, and add bubble to cause instrument to be not easy to enter steady state (SS), analyst is difficult for grasping its operation.
Summary of the invention
For overcoming above-mentioned deficiency, the invention provides a kind of instrument simple, saved chemical reagent, avoided mensuration reducing sugar and the automatic analyzer of Water-soluble Total Sugar content and the assay method easily and fast of toxic reagent to operating personnel's infringement.
Measure an automatic analyzer for reducing sugar and Water-soluble Total Sugar content, wherein, comprise sampling device and color developing detection device, described sampling device comprises automatic sampler, the first peristaltic pump, described the first peristaltic pump is provided with the first pump line, the sample introduction needle of described automatic sampler is connected with the F port of multiport valve by the first pump line, and the A port of multiport valve is connected with waste liquid tank, between be provided with the first back pressure regulator, described color developing detection device, comprise the second peristaltic pump, described the second peristaltic pump is provided with the second pump line, the 3rd pump line, the 4th pump line, the 5th pump line, the 6th pump line, the 7th pump line, the two ends of sampling ring are connected to B port and the E port of described multiport valve, the D port of described multiport valve is connected by described the second pump line with current-carrying solution bottle, the C port of described multiport valve, the first threeway, on-line heating is cleared up module, the second threeway, the first knitting reactor, the 3rd threeway, the 4th threeway, on-line heating colour developing module, the 5th threeway, the second knitting reactor, flow cell, the second back pressure regulator, waste liquid tank is connected successively by connecting capillary, detecting device, data stube cable are connected successively with computer processing system, described the first threeway is connected with second container bottle by the 3rd pump line, described the second threeway is connected with the 3rd container bottle by described the 4th pump line, described the 3rd threeway is connected with the 4th container bottle by described the 5th pump line, described the 4th threeway is connected with the 5th container bottle by described the 6th pump line, and described the 5th threeway is connected with the 6th container bottle by described the 7th pump line.
The automatic analyzer of reducing sugar of the present invention and Water-soluble Total Sugar, wherein, the described first to the 7th pump line internal diameter is 0.38～1.85mm.
The automatic analyzer of reducing sugar of the present invention and Water-soluble Total Sugar, wherein, described the first peristaltic pump, the second peristaltic pump pump speed are 15～60 revs/min.
The automatic analyzer of reducing sugar of the present invention and Water-soluble Total Sugar, wherein, described sampling ring capillary pipe length is 1～4m; The first knitting reactor capillary pipe length is 0.5～3m; The second knitting reactor capillary pipe length is 1～4m; It is 5～10m that on-line heating is cleared up module capillary pipe length; On-line heating colour developing module capillary pipe length is 4～9m.
The automatic analyzer of reducing sugar of the present invention and Water-soluble Total Sugar, wherein, the internal diameter of connecting capillary is 0.5～1.0mm.
The method of using the automatic analyzer detection reducing sugar of any one reducing sugar of the present invention and Water-soluble Total Sugar, wherein, in turn includes the following steps:
(1) deionized water in liquid to be detected and second container bottle mixes by the first threeway, and mixed liquor injects online heating and decompose module, and the temperature that on-line heating is cleared up module is 80-120 ℃;
(2) liquid and the deionized water of step (1) gained are mixed in the second knitting reactor by the second threeway;
(3) sodium hydroxide solution that the liquid of step (2) step gained is 0.1～1.5mol/L with concentration mixes by the 3rd threeway, after mixing and nitrite ion by the common Input Online heating of the 4th threeway colour developing module, the on-line heating module temperature that develops the color is 60～100 ℃;
(4) mixed liquor of step (3) step gained and catalyzer are mixed in the second knitting reactor by the 5th threeway;
(5) mixed liquor of step (4) step gained is entered to optical flow cell, produce spectrogram and surveyed and drawn, waste liquid enters waste liquid bottle through back pressure regulator;
(6) the sample spectrogram of step (6) being drawn and the comparison of standard specimen spectrogram, calculate the concentration of reducing sugar in sample.
The method of detection reducing sugar of the present invention, wherein, current-carrying solution is deionized water; In chromogenic reagent solution, para hydroxybenzene formylhydrazine concentration is 25～100g/L, and citric acid concentration is 10～50g/L, and concentration of hydrochloric acid solution is 0.3～1.0mol/L; Described catalyzer is that concentration is 0.002～0.05mol/L calcium salt soln, cadmium salt soln or bismuth salt solusion, and the light path of flow cell is 10-50mm, and the detection wavelength of detecting device is 370～450nm.
The method of detection reducing sugar of the present invention, wherein, described calcium salt is lime chloride.
The method of using the automatic analyzer detection Water-soluble Total Sugar of any one reducing sugar of the present invention and Water-soluble Total Sugar, wherein, in turn includes the following steps:
(1) hydrochloric acid in liquid to be detected and second container bottle mixes by the first threeway, and mixed liquor injects online heating and decompose module, and the temperature that on-line heating is cleared up module is 80～120 ℃;
(2) by liquid and 0.5～2.5mol/L sodium hydroxide solution of step (1) gained, by the second threeway, in the second knitting reactor, mix;
(3) sodium hydroxide solution that the liquid of step (2) step gained is 0.1～1.5mol/L with concentration mixes by the 3rd threeway, after mixing and nitrite ion by the common Input Online heating of the 4th threeway colour developing module, the on-line heating module temperature that develops the color is 60～100 ℃;
(4) mixed liquor of step (3) step gained and catalyzer are mixed in the second knitting reactor by the 5th threeway;
(5) mixed liquor of step (4) step gained is entered to optical flow cell, produce spectrogram and surveyed and drawn, waste liquid enters waste liquid bottle through back pressure regulator;
(6) the sample spectrogram of step (5) being drawn and the comparison of standard specimen spectrogram, calculate the concentration of Water-soluble Total Sugar in sample.
The method of detection Water-soluble Total Sugar of the present invention, wherein, current-carrying solution is deionized water; Described concentration of hydrochloric acid solution is 0.5-2mol/L; In chromogenic reagent solution, para hydroxybenzene formylhydrazine concentration is 25～100g/L, and citric acid concentration is 10～50g/L, and concentration of hydrochloric acid solution is 0.5～2.0mol/L; Described catalyzer is that concentration is 0.002～0.05mol/L calcium salt soln, cadmium salt soln, bismuth salt solusion, and the light path of flow cell is 10-50mm, and the detection wavelength of detecting device is 370～450nm.
The invention has the beneficial effects as follows:
1, use this kind of method and supporting analytical instrument thereof, compare with domestic existing manual operations, automaticity is high, easily and quickly the online automatic detection of carrying out to reducing sugar in water sample and Water-soluble Total Sugar content.
2, the present invention be take standard method (YC/T 159-2002) as basis, with Using Flow Injection Analysis, replaces Continuous Flow Analysis method, and instrument is simple, has saved chemical reagent, has avoided the infringement of toxic reagent to operating personnel.
3, by test of many times, optimized differential responses stage knitting reactor length capillaceous and caliber, made its response obtain maximal value.
Accompanying drawing explanation
Fig. 1 is the structural representation of mensuration reducing sugar of the present invention and Water-soluble Total Sugar automatic analyzer.
Embodiment
Below in conjunction with Figure of description, the automatic analyzer of reducing sugar of the present invention and Water-soluble Total Sugar is described further.
The present embodiment 1
Referring to Fig. 1, the automatic analyzer of mensuration reducing sugar of the present invention and Water-soluble Total Sugar content, comprises sampling device and color developing detection device, sampling device comprises automatic sampler 1, the first peristaltic pump 11, the first peristaltic pump 11 is provided with the first pump line 12, the sample introduction needle 2 of automatic sampler 1 is connected with the F port of multiport valve 20 by the first pump line 12, and the A port of multiport valve 20 is connected with waste liquid tank 36, between be provided with the first back pressure regulator 21, color developing detection device, comprise the second peristaltic pump 4, the second peristaltic pump 4 is provided with the second pump line 13, the 3rd pump line 14, the 4th pump line 15, the 5th pump line 16, the 6th pump line 17, the 7th pump line 18, the two ends of sampling ring 19 are connected to B port and the E port of multiport valve 20, the D port of multiport valve 20 is connected by the second pump line 13 with current-carrying solution bottle 5, the C port of multiport valve 20, the first threeway 22, on-line heating is cleared up module 23, the second threeway 24, the first knitting reactor 25, the 3rd threeway 26, the 4th threeway 28, on-line heating colour developing module 27, the 5th threeway 29, the second knitting reactor 30, flow cell 32, the second back pressure regulator 31, waste liquid tank 36 connects successively by connecting capillary, detecting device 33, data stube cable 35 and computer processing system 34 are connected successively, the first threeway 22 is connected with second container bottle 6 by the 3rd pump line 14, the second threeway 24 is connected with the 3rd container bottle 7 by the 4th pump line 15, the 3rd threeway 26 is connected with the 4th container bottle 8 by the 5th pump line 16, the 4th threeway 28 is connected with the 5th container bottle 9 by the 6th pump line 17, and the 5th threeway 29 is connected with the 6th container bottle 10 by the 7th pump line 18.The first to the 7th pump line internal diameter is 0.38～1.85mm, the first peristaltic pump, the second peristaltic pump pump speed are 15～60 revs/min, sampling ring capillary pipe length is 1～4m, the first knitting reactor capillary pipe length is 0.5～3m, the second knitting reactor capillary pipe length is 1～4m, it is 5～10m that on-line heating is cleared up module capillary pipe length, and on-line heating colour developing module capillary pipe length is 4～9m, and the internal diameter of connecting capillary is 0.5～1.0mm.
The course of work of automatic analyzer that the present invention measures reducing sugar and Water-soluble Total Sugar content is as follows:
Referring to Fig. 1, sample liquid to be measured after pre-treatment is placed in sample hose 3, by automatic sampler 1 under the effect of the first peristaltic pump 11, sample enters in the sampling ring 19 connecting on six-way valve 20 by sample introduction needle 2, after sampling ring 19 is full of, excessive part directly enters waste liquid bottle 36 through the first back pressure regulator 21.After sampling process finishes, six-way valve 20 turns to the state shown in dotted line in figure, current-carrying in the first container bottle 5 is under the effect of the second peristaltic pump 4, sample in sampling ring 19 is released and mixed with the deionized water in second container bottle 6 by the first threeway 22, enter afterwards on-line heating and clear up module 23, mixed solution after heating and decompose mixes with deionized water in the 3rd container bottle 7 by the second threeway 24 in the first knitting reactor 25, solution after mixing mixes with the sodium hydroxide solution of 0.1mol/L in the 4th container bottle 8 by the 3rd threeway 26, by the 4th threeway 28, mix with the 5th container bottle 9 chromogenic reagent solutions more afterwards, after mixing, enter on-line heating colour developing module 27, from heating display device 27 solution out, by the 5th threeway 29, mix the second knitting reactor 30 with calcium chloride solution 10, pond 32 directly circulates after mixing, by detecting device 33, carry out colorimetric estimation, the liquid flowing out directly enters waste liquid bottle 36 through the second back pressure regulator 31.
The course of work of automatic analyzer that the present invention measures reducing sugar and Water-soluble Total Sugar content is as follows:
Referring to Fig. 1, sample liquid to be measured after pre-treatment is placed in sample hose 3, by automatic sampler 1 under the effect of the first peristaltic pump 11, sample enters in the sampling ring 19 connecting on six-way valve 20 by sample introduction needle 2, after sampling ring 19 is full of, excessive part directly enters waste liquid bottle 36 through the first back pressure regulator 21.After sampling process finishes, six-way valve 20 turns to the state shown in dotted line in figure, current-carrying in the first container bottle 5 is under the effect of the second peristaltic pump 4, sample in sampling ring 19 is released by the mixed in hydrochloric acid in the first threeway 22 and second container bottle 6, enter afterwards on-line heating and clear up module 23, mixed solution after heating and decompose mixes with the sodium hydroxide solution of 0.5mol/L in the 3rd container bottle 7 by the second threeway 24 in the first knitting reactor 25, solution after mixing mixes with the sodium hydroxide solution of 0.1mol/L in the 4th container bottle 8 by the 3rd threeway 26, by the 4th threeway 28, mix with the 5th container bottle 9 chromogenic reagent solutions more afterwards, after mixing, enter on-line heating colour developing module 27, from heating display device 27 solution out, by the 5th threeway 29, mix the second knitting reactor 30 with calcium chloride solution 10, pond 32 directly circulates after mixing, by detecting device 33, carry out colorimetric estimation, the liquid flowing out directly enters waste liquid bottle 36 through the second back pressure regulator 31.
The present embodiment 2
Use the automatic analyzer of reducing sugar and Water-soluble Total Sugar, the first to the 7th pump pump line internal diameter is 0.38mm, the first peristaltic pump, the second peristaltic pump pump speed are 15 revs/min, described sampling ring capillary pipe length is 1m, the first knitting reactor capillary pipe length is 0.5m, and the second knitting reactor capillary pipe length is 1m, and it is 5m that on-line heating is cleared up module capillary pipe length, on-line heating colour developing module capillary pipe length is 4m, and the internal diameter of connecting capillary is 0.5mm.The method of measuring reducing sugar, in turn includes the following steps:
(1) deionized water in liquid to be detected and second container bottle 6 mixes by the first threeway, and mixed liquor injects online heating and decompose module 23, and the temperature that on-line heating is cleared up module is 80 ℃;
(2) liquid of step (1) gained and deionized water are mixed in the second knitting reactor 25 by the second threeway;
(3) sodium hydroxide solution that the liquid of step (2) step gained is 0.1mol/L with concentration mixes by the 3rd threeway, after mixing and nitrite ion by the common Input Online heating of the 4th threeway colour developing module 27, the on-line heating module temperature 27 that develops the color is 60 ℃;
(4) mixed liquor of step (3) step gained and catalyzer are mixed in the second knitting reactor 30 by the 5th threeway;
(5) mixed liquor of step (4) step gained is entered to optical flow cell 32, produce spectrogram and surveyed and drawn, waste liquid enters waste liquid bottle 36 through back pressure regulator 31;
(6) the sample spectrogram of step (5) being drawn and the comparison of standard specimen spectrogram, calculate the concentration of reducing sugar in sample.
Current-carrying solution is deionized water; In chromogenic reagent solution, para hydroxybenzene formylhydrazine concentration is 25g/L, and citric acid concentration is 10g/L, and concentration of hydrochloric acid solution is 0.3mol/L; Catalyzer is that concentration is 0.002mol/L calcium chloride solution, and the light path of flow cell is 10mm, and the detection wavelength of detecting device is 370nm.
Table 1 adopts method and the test result of instrument to standard sample of the present embodiment 2:
From this embodiment, adopt this method to record result accurate.
The present embodiment 3
The automatic analyzer of reducing sugar and Water-soluble Total Sugar, the described first to the 7th pump pump line internal diameter is 1.85mm, described the first peristaltic pump, the second peristaltic pump pump speed are 60 revs/min, described sampling ring capillary pipe length is 4m, the first knitting reactor capillary pipe length is 3m, and the second knitting reactor capillary pipe length is 4m, and it is 10m that on-line heating is cleared up module capillary pipe length, on-line heating colour developing module capillary pipe length is 9m, and the internal diameter of connecting capillary is 1.0mm.The method of measuring reducing sugar, in turn includes the following steps:
(1) deionized water in liquid to be detected and container bottle (6) mixes by the first threeway, and mixed liquor injects online heating and decompose module (23), and the temperature that on-line heating is cleared up module is 120 ℃;
(2) liquid of step (1) gained and deionized water are mixed in the second knitting reactor 25 by the second threeway;
(3) sodium hydroxide solution that the liquid of step (2) step gained is 1.5mol/L with concentration mixes by the 3rd threeway, after mixing and nitrite ion by the common Input Online heating of the 4th threeway colour developing module 27, the on-line heating module temperature 27 that develops the color is 100 ℃;
(4) mixed liquor of step (3) step gained and catalyzer are mixed in the second knitting reactor 30 by the 5th threeway;
(5) mixed liquor of step (4) step gained is entered to optical flow cell 32, produce spectrogram and surveyed and drawn, waste liquid enters waste liquid bottle 36 through back pressure regulator 31;
(6) the sample spectrogram of step (5) being drawn and the comparison of standard specimen spectrogram, calculate the concentration of reducing sugar in sample.
Current-carrying solution is deionized water; In chromogenic reagent solution, para hydroxybenzene formylhydrazine concentration is 100g/L, and citric acid concentration is 50g/L, and concentration of hydrochloric acid solution is 1.0mol/L; Catalyzer is that concentration is that the light path of 0.05mol/L calcium chloride solution flow cell is 50mm, and the detection wavelength of detecting device is 450nm.
Table 2 adopts method and the test result of instrument to standard sample of the present embodiment 3:
From this embodiment, adopt this method to record result accurate.
The present embodiment 4
The automatic analyzer of reducing sugar and Water-soluble Total Sugar, the described first to the 7th pump pump line internal diameter is 1.1mm, described the first peristaltic pump, the second peristaltic pump pump speed are 40 revs/min, described sampling ring capillary pipe length is 3m, the first knitting reactor capillary pipe length is 2m, and the second knitting reactor capillary pipe length is 2m, and it is 7m that on-line heating is cleared up module capillary pipe length, on-line heating colour developing module capillary pipe length is 6m, and the internal diameter of connecting capillary is 0.7mm.The method of measuring reducing sugar, in turn includes the following steps:
(1) deionized water in liquid to be detected and container bottle (6) mixes by the first threeway, and mixed liquor injects online heating and decompose module (23), and the temperature that on-line heating is cleared up module is 100 ℃;
(2) liquid of step (1) gained and deionized water are mixed in the second knitting reactor 25 by the second threeway;
(3) sodium hydroxide solution that the liquid of step (2) step gained is 1.2mol/L with concentration mixes by the 3rd threeway, after mixing and nitrite ion by the common Input Online heating of the 4th threeway colour developing module 27, the on-line heating module temperature 27 that develops the color is 80 ℃;
(4) mixed liquor of step (3) step gained and catalyzer are mixed in the second knitting reactor 30 by the 5th threeway;
(5) mixed liquor of step (4) step gained is entered to optical flow cell 32, produce spectrogram and surveyed and drawn, waste liquid enters waste liquid bottle 36 through back pressure regulator 31;
(6) the sample spectrogram of step (5) being drawn and the comparison of standard specimen spectrogram, calculate the concentration of reducing sugar in sample.
Current-carrying solution is deionized water; In chromogenic reagent solution, para hydroxybenzene formylhydrazine concentration is 60g/L, and citric acid concentration is 30g/L, and concentration of hydrochloric acid solution is 0.6mol/L; Catalyzer is that concentration is 0.026mol/L cadmium chloride solution, and the light path of flow cell is 30mm, and the detection wavelength of detecting device is 410nm.
Table 3 adopts method and the test result of instrument to standard sample of the present embodiment 4:
From this embodiment, adopt this method to record result accurate.
The present embodiment 5
Use the automatic analyzer of reducing sugar and Water-soluble Total Sugar, the first to the 7th pump pump line internal diameter is 0.38mm, described the first peristaltic pump, the second peristaltic pump pump speed are 15 revs/min, described sampling ring capillary pipe length is 1m, the first knitting reactor capillary pipe length is 0.5m, and the second knitting reactor capillary pipe length is 1m, and it is 5m that on-line heating is cleared up module capillary pipe length, on-line heating colour developing module capillary pipe length is 4m, and the internal diameter of connecting capillary is 0.5mm.The method of measuring Water-soluble Total Sugar in turn includes the following steps:
(1) hydrochloric acid water in liquid to be detected and container bottle 6 mixes by the first threeway, and mixed liquor injects online heating and decompose module 23, and the temperature that on-line heating is cleared up module is 80 ℃;
(2) NaOH of the liquid of step (1) gained and 0.5mol/L is mixed in the second knitting reactor 25 by the second threeway;
(3) sodium hydroxide solution that the liquid of step (2) step gained is 0.1mol/L with concentration mixes by the 3rd threeway, after mixing and nitrite ion by the common Input Online heating of the 4th threeway colour developing module 27, the on-line heating module temperature 27 that develops the color is 60 ℃;
(4) mixed liquor of step (3) step gained and catalyzer are mixed in the second knitting reactor 30 by the 5th threeway;
(5) mixed liquor of step (4) step gained is entered to optical flow cell 32, produce spectrogram and surveyed and drawn, waste liquid enters waste liquid bottle 36 through back pressure regulator 31;
(6) the sample spectrogram of step (5) being drawn and the comparison of standard specimen spectrogram, calculate the concentration of reducing sugar in sample.Current-carrying solution is deionized water; Concentration of hydrochloric acid solution is 0.5mol/L; In chromogenic reagent solution, para hydroxybenzene formylhydrazine concentration is 25g/L, and citric acid concentration is 10g/L, and concentration of hydrochloric acid solution is 0.3mol/L, and catalyzer is that concentration is 0.002mol/L calcium chloride solution, and the light path of flow cell is 10mm, and the detection wavelength of detecting device is 370nm.
Table 4 adopts method and the test result of instrument to standard sample of the present embodiment 5:
From this embodiment, adopt this method to record result accurate.
The present embodiment 6
The automatic analyzer of reducing sugar and Water-soluble Total Sugar, the described first to the 7th pump pump line internal diameter is 1.85mm, described the first peristaltic pump, the second peristaltic pump pump speed are 60 revs/min, described sampling ring capillary pipe length is 4m, the first knitting reactor capillary pipe length is 3m, and the second knitting reactor capillary pipe length is 4m, and it is 10m that on-line heating is cleared up module capillary pipe length, on-line heating colour developing module capillary pipe length is 9m, and the internal diameter of connecting capillary is 1.0mm.The method of measuring Water-soluble Total Sugar, in turn includes the following steps:
(1) hydrochloric acid water in liquid to be detected and container bottle 6 mixes by the first threeway, and mixed liquor injects online heating and decompose module 23, and the temperature that on-line heating is cleared up module is 80 ℃;
(2) NaOH of the liquid of step (1) gained and 1.5mol/L is mixed in the second knitting reactor 25 by the second threeway;
(3) sodium hydroxide solution that the liquid of step (2) step gained is 1.5mol/L with concentration mixes by the 3rd threeway, after mixing and nitrite ion by the common Input Online heating of the 4th threeway colour developing module 27, the on-line heating module temperature 27 that develops the color is 100 ℃;
(4) mixed liquor of step (3) step gained and catalyzer are mixed in the second knitting reactor 30 by the 5th threeway;
(5) mixed liquor of step (4) step gained is entered to optical flow cell 32, produce spectrogram and surveyed and drawn, waste liquid enters waste liquid bottle 36 through back pressure regulator 31;
(6) the sample spectrogram of step (5) being drawn and the comparison of standard specimen spectrogram, calculate the concentration of reducing sugar in sample.Current-carrying solution is deionized water; Concentration of hydrochloric acid solution is 2mol/L; In chromogenic reagent solution, para hydroxybenzene formylhydrazine concentration is 100g/L, and citric acid concentration is 50g/L, and concentration of hydrochloric acid solution is 1.0mol/L; Catalyzer is that concentration is that the light path of 0.05mol/L cadmium chloride solution flow cell is 50mm, and the detection wavelength of detecting device is 450nm.
Table 5 adopts method and the test result of instrument to standard sample of the present embodiment 6:
From this embodiment, adopt this method to record result accurate.
The present embodiment 7
The automatic analyzer of reducing sugar and Water-soluble Total Sugar, the described first to the 7th pump pump line internal diameter is 1.1mm, described the first peristaltic pump, the second peristaltic pump pump speed are 40 revs/min, described sampling ring capillary pipe length is 3m, the first knitting reactor capillary pipe length is 2m, and the second knitting reactor capillary pipe length is 2m, and it is 7m that on-line heating is cleared up module capillary pipe length, on-line heating colour developing module capillary pipe length is 6m, and the internal diameter of connecting capillary is 0.7mm.The method of measuring Water-soluble Total Sugar, in turn includes the following steps:
(1) hydrochloric acid water in liquid to be detected and container bottle (6) mixes by the first threeway, and mixed liquor injects online heating and decompose module 23, and the temperature that on-line heating is cleared up module is 80 ℃;
(2) NaOH of the liquid of step (1) gained and 1.5mol/L is mixed in the second knitting reactor 25 by the second threeway;
(3) sodium hydroxide solution that the liquid of step (2) step gained is 1.2mol/L with concentration mixes by the 3rd threeway, after mixing and nitrite ion by the common Input Online heating of the 4th threeway colour developing module 27, the on-line heating module temperature 27 that develops the color is 80 ℃;
(4) mixed liquor of step (3) step gained and catalyzer are mixed in the second knitting reactor 30 by the 5th threeway;
(5) mixed liquor of step (4) step gained is entered to optical flow cell 32, produce spectrogram and surveyed and drawn, waste liquid enters waste liquid bottle 36 through back pressure regulator 31;
(6) the sample spectrogram of step (5) being drawn and the comparison of standard specimen spectrogram, calculate the concentration of reducing sugar in sample.Current-carrying solution is deionized water; Concentration of hydrochloric acid solution is 1.2mol/L; In chromogenic reagent solution, para hydroxybenzene formylhydrazine concentration is 60g/L, and citric acid concentration is 30g/L, and concentration of hydrochloric acid solution is 0.6mol/L; Catalyzer is that concentration is 0.026mol/L bismuth chloride solution, and the light path of flow cell is 30mm, and the detection wavelength of detecting device is 410nm.
Table 6 adopts method and the test result of instrument to standard sample of the present embodiment 7:
From this embodiment, adopt this method to record result accurate.
Above embodiment is described the preferred embodiment of the present invention; not scope of the present invention is limited; design under the prerequisite of spirit not departing from the present invention; various distortion and improvement that those of ordinary skills make technical scheme of the present invention, all should fall in the definite protection domain of the claims in the present invention book.
Claims (5)
1. the method for using the automatic analyzer detection reducing sugar of measuring reducing sugar and Water-soluble Total Sugar content, described automatic analyzer comprises sampling device and color developing detection device, described sampling device comprises automatic sampler (1), the first peristaltic pump (11), described the first peristaltic pump (11) is provided with the first pump line (12), the sample introduction needle (2) of described automatic sampler (1) is connected with the F port of multiport valve (20) by the first pump line (12), the A port of multiport valve (20) is connected with waste liquid tank (36), between the A port of multiport valve (20) and waste liquid tank (36), is provided with the first back pressure regulator (21), described color developing detection device, comprise the second peristaltic pump (4), described the second peristaltic pump (4) is provided with the second pump line (13), the 3rd pump line (14), the 4th pump line (15), the 5th pump line (16), the 6th pump line (17), the 7th pump line (18), the two ends of sampling ring (19) are connected to B port and the E port of described multiport valve (20), the D port of described multiport valve (20) is connected by described the second pump line (13) with current-carrying solution bottle (5), the C port of described multiport valve (20), the first threeway (22), on-line heating is cleared up module (23), the second threeway (24), the first knitting reactor (25), the 3rd threeway (26), the 4th threeway (28), on-line heating colour developing module (27), the 5th threeway (29), the second knitting reactor (20), flow cell (32), the second back pressure regulator (31), waste liquid tank (36) connects successively by connecting capillary, detecting device (33), data stube cable (35) and computer processing system (34) are connected successively, described the first threeway (22) is connected with second container bottle (6) by the 3rd pump line (14), described the second threeway (24) is connected with the 3rd container bottle (7) by described the 4th pump line (15), described the 3rd threeway (26) is connected with the 4th container bottle (8) by described the 5th pump line (16), described the 4th threeway (28) is connected with the 5th container bottle (9) by described the 6th pump line (17), described the 5th threeway (29) is connected with the 6th container bottle (10) by described the 7th pump line (18), it is characterized in that, in turn include the following steps:
(1) deionized water in liquid to be detected and second container bottle (6) mixes by the first threeway, and mixed liquor injects online heating and decompose module (23), and the temperature that on-line heating is cleared up module is 80-120 ℃;
(2) liquid of step (1) gained and deionized water are mixed in the first knitting reactor (25) by the second threeway;
(3) sodium hydroxide solution that the liquid of step (2) step gained is 0.1～1.5mol/L with concentration mixes by the 3rd threeway, after mixing and nitrite ion by the common Input Online heating of the 4th threeway colour developing module (27), the on-line heating module temperature (27) that develops the color is 60～100 ℃;
(4) mixed liquor of step (3) step gained and catalyzer are mixed in the second knitting reactor (30) by the 5th threeway;
(5) mixed liquor of step (4) step gained is entered to optical flow cell (32), produce spectrogram and surveyed and drawn, waste liquid enters waste liquid bottle (36) through the second back pressure regulator (31);
(6) the sample spectrogram of step (5) being drawn and the comparison of standard specimen spectrogram, calculate the concentration of reducing sugar in sample.
2. the method for detection reducing sugar according to claim 1, is characterized in that, the current-carrying solution in described current-carrying solution bottle (5) is deionized water; In nitrite ion, para hydroxybenzene formylhydrazine concentration is 25～100g/L, and citric acid concentration is 10～50g/L, and concentration of hydrochloric acid solution is 0.3～1.0mol/L; Described catalyzer is that concentration is 0.002～0.05mol/L calcium salt soln, cadmium salt soln or bismuth salt solusion, and the light path of flow cell is 10-50mm, and the detection wavelength of detecting device is 370～450nm.
3. the method for detection reducing sugar according to claim 2, is characterized in that, described calcium salt is lime chloride.
4. the method for using the automatic analyzer detection Water-soluble Total Sugar of measuring reducing sugar and Water-soluble Total Sugar content, described automatic analyzer comprises sampling device and color developing detection device, described sampling device comprises automatic sampler (1), the first peristaltic pump (11), described the first peristaltic pump (11) is provided with the first pump line (12), the sample introduction needle (2) of described automatic sampler (1) is connected with the F port of multiport valve (20) by the first pump line (12), the A port of multiport valve (20) is connected with waste liquid tank (36), between the A port of multiport valve (20) and waste liquid tank (36), is provided with the first back pressure regulator (21), described color developing detection device, comprise the second peristaltic pump (4), described the second peristaltic pump (4) is provided with the second pump line (13), the 3rd pump line (14), the 4th pump line (15), the 5th pump line (16), the 6th pump line (17), the 7th pump line (18), the two ends of sampling ring (19) are connected to B port and the E port of described multiport valve (20), the D port of described multiport valve (20) is connected by described the second pump line (13) with current-carrying solution bottle (5), the C port of described multiport valve (20), the first threeway (22), on-line heating is cleared up module (23), the second threeway (24), the first knitting reactor (25), the 3rd threeway (26), the 4th threeway (28), on-line heating colour developing module (27), the 5th threeway (29), the second knitting reactor (20), flow cell (32), the second back pressure regulator (31), waste liquid tank (36) connects successively by connecting capillary, detecting device (33), data stube cable (35) and computer processing system (34) are connected successively, described the first threeway (22) is connected with second container bottle (6) by the 3rd pump line (14), described the second threeway (24) is connected with the 3rd container bottle (7) by described the 4th pump line (15), described the 3rd threeway (26) is connected with the 4th container bottle (8) by described the 5th pump line (16), described the 4th threeway (28) is connected with the 5th container bottle (9) by described the 6th pump line (17), described the 5th threeway (29) is connected with the 6th container bottle (10) by described the 7th pump line (18), it is characterized in that, in turn include the following steps:
(1) hydrochloric acid in liquid to be detected and second container bottle (6) mixes by the first threeway, and mixed liquor injects online heating and decompose module (23), and the temperature that on-line heating is cleared up module is 80～120 ℃;
(2), by liquid and 0.5～2.5mol/L sodium hydroxide solution of step (1) gained, by the second threeway, in the first knitting reactor (25), mix;
(3) sodium hydroxide solution that the liquid of step (2) step gained is 0.1～1.5mol/L with concentration mixes by the 3rd threeway, after mixing and nitrite ion by the common Input Online heating of the 4th threeway colour developing module (27), the on-line heating module temperature (27) that develops the color is 60～100 ℃;
(4) mixed liquor of step (3) step gained and catalyzer are mixed in the second knitting reactor (30) by the 5th threeway;
(5) mixed liquor of step (4) step gained is entered to optical flow cell (32), produce spectrogram and surveyed and drawn, waste liquid enters waste liquid bottle (36) through the second back pressure regulator (31);
(6) the sample spectrogram of step (5) being drawn and the comparison of standard specimen spectrogram, calculate the concentration of Water-soluble Total Sugar in sample.
5. the method for detection Water-soluble Total Sugar according to claim 4, is characterized in that, the current-carrying solution in described current-carrying solution bottle (5) is deionized water; Described concentration of hydrochloric acid solution is 0.5-2mol/L; In nitrite ion, para hydroxybenzene formylhydrazine concentration is 25～100g/L, and citric acid concentration is 10～50g/L, and concentration of hydrochloric acid solution is 0.5～2.0mol/L; Described catalyzer is that concentration is 0.002～0.05mol/L calcium salt soln, cadmium salt soln, bismuth salt solusion, and the light path of flow cell is 10-50mm, and the detection wavelength of detecting device is 370～450nm.
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| CN103529021A (en) * | 2012-07-03 | 2014-01-22 | 山东省科学院生物研究所 | Quick analyzing method of total sugar |
| CN102830117A (en) * | 2012-09-18 | 2012-12-19 | 国家海洋局天津海水淡化与综合利用研究所 | Device and method for carrying out continuous flow detection on bicarbonates in water |
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|---|---|---|---|---|
| CN1699976A (en) * | 2005-07-15 | 2005-11-23 | 北京吉天仪器有限公司 | Flow injection colorimetric cyanogens detection device and cyanogens detection method thereof |
| CN101692093A (en) * | 2009-09-28 | 2010-04-07 | 北京吉天仪器有限公司 | Automatic analyzer for anionic surfactant in water and automatic analysis method |
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|---|---|---|---|---|
| CN1699976A (en) * | 2005-07-15 | 2005-11-23 | 北京吉天仪器有限公司 | Flow injection colorimetric cyanogens detection device and cyanogens detection method thereof |
| CN101692093A (en) * | 2009-09-28 | 2010-04-07 | 北京吉天仪器有限公司 | Automatic analyzer for anionic surfactant in water and automatic analysis method |
Non-Patent Citations (3)
| Title |
|---|
| JP平1-245161A 1989.09.29 |
| 蜂蜜和饮料中还原糖含量的流动注射分光光度测定法;陈婷等;《中国食品学报》;20091231;第9卷(第6期);171 * |
| 陈婷等.蜂蜜和饮料中还原糖含量的流动注射分光光度测定法.《中国食品学报》.2009,第9卷(第6期),171. |
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