CN102262059A - Automatic analysis meter and methods for reducing sugar content and water-soluble total sugar content - Google Patents
Automatic analysis meter and methods for reducing sugar content and water-soluble total sugar content Download PDFInfo
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Abstract
The invention relates to an automatic analysis meter and methods for measuring the reducing sugar content and the water-soluble total sugar content. The automatic analysis meter comprises a sample injection device and a color development detection device, wherein the sample injection device comprises an automatic sample injector and a first peristaltic pump; the first peristaltic pump is provided with a first pump pipe; a sample injection needle of the automatic sample injector is connected with a through opening F of a multi-way valve through the first pump pipe; a through opening A of the multi-way valve is connected with a waste liquid trough; a first back pressure adjuster is arranged between the through opening A of the multi-way valve and the waste liquid trough; the color development detection device comprises a second peristaltic pump provided with a second pump pipe, a third pump pipe, a fourth pump pipe, a fifth pump pipe, a sixth pump pipe and a seventh pump pipe; both ends of a sampling ring are connected with a through opening B and a through opening E of the multi-way valve; a through opening D of the multi-way valve is connected with flow carrying solution through the second pump pipe; and a through opening C of the multi-way valve is connected with a first tee joint, an online heating digestion module, a second tee joint, a first knotted reactor, a third tee joint, a fourth tee joint, an online heating color development module, a fifth tee joint, a second knotted reactor, a flow cell, a detector, a data connection cable and a computer processing system respectively. The automatic analysis meter can automatically and continuously test the sample accurately and quickly.
Description
Technical field
The present invention relates to a kind of full-automatic flow injection analyzer, relate in particular to the automatic analyzer and the analytical approach thereof of a kind of reducing sugar and water-soluble total sugar content.
Background technology
At present, the reducing sugar in the materials such as food, plant and the mensuration of water-soluble total sugar content, many dependence manual operationss, its complex steps, personal error is bigger, and precision is poor, and test speed is slow, and toxic reagent may cause damage to operating personnel.Use at present high performance liquid chromatograph can measure reducing sugar, but can't directly measure water-soluble total sugar content, and instrument costs an arm and a leg, test speed is slow.At present, commercial bubble Continuous Flow Analysis instrument is at interval arranged on the market, can directly measure reducing sugar and water-soluble sugar contents, but need have extra bubble adding apparatus, the apparatus structure complexity, cost an arm and a leg, and add bubble and cause instrument to be not easy to enter steady state (SS), the analyst is difficult for grasping its operation.
Summary of the invention
For overcoming above-mentioned deficiency, it is simple to the invention provides a kind of instrument, has saved chemical reagent, has avoided mensuration reducing sugar and the automatic analyzer of water-soluble total sugar content and easily and fast the assay method of toxic reagent to operating personnel's infringement.
A kind of automatic analyzer of measuring reducing sugar and water-soluble total sugar content wherein, comprises sampling device and color developing detection device; Described sampling device comprises automatic sampler, first peristaltic pump, described first peristaltic pump is provided with first pump line, the sample introduction needle of described automatic sampler is connected with the F port of multiport valve by first pump line, and the A port of multiport valve is connected with waste liquid tank, between be provided with first back pressure regulator; Described color developing detection device, comprise second peristaltic pump, described second peristaltic pump is provided with second pump line, the 3rd pump line, the 4th pump line, the 5th pump line, the 6th pump line, the 7th pump line, the two ends of sampling ring are connected the B port and the E port of described multiport valve, the D port of described multiport valve is connected by described second pump line with current-carrying solution bottle, the C port of described multiport valve, first threeway, module is cleared up in online heating, second threeway, first knitting reactor, the 3rd threeway, the 4th threeway, online heating colour developing module, the 5th threeway, second knitting reactor, flow cell, second back pressure regulator, waste liquid tank links to each other successively by connecting capillary; Detecting device, data stube cable are connected successively with computer processing system; Described first threeway links to each other with second container bottle by the 3rd pump line, described second threeway links to each other with the 3rd container bottle by described the 4th pump line, described the 3rd threeway links to each other with the 4th container bottle by described the 5th pump line, described the 4th threeway links to each other with the 5th container bottle by described the 6th pump line, and described the 5th threeway links to each other with the 6th container bottle by described the 7th pump line.
The automatic analyzer of reducing sugar of the present invention and water-soluble total reducing sugar, wherein, described first to the 7th pump line internal diameter is 0.38～1.85mm.
The automatic analyzer of reducing sugar of the present invention and water-soluble total reducing sugar, wherein, described first peristaltic pump, the second peristaltic pump pump speed are 15～60 rev/mins.
The automatic analyzer of reducing sugar of the present invention and water-soluble total reducing sugar, wherein, described sampling ring capillary pipe length is 1～4m; The first knitting reactor capillary pipe length is 0.5～3m; The second knitting reactor capillary pipe length is 1～4m; It is 5～10m that the module capillary pipe length is cleared up in online heating; Online heating colour developing module capillary pipe length is 4～9m.
The automatic analyzer of reducing sugar of the present invention and water-soluble total reducing sugar, wherein, the internal diameter of connecting capillary is 0.5～1.0mm.
Use the method for the automatic analyzer detection reducing sugar of any reducing sugar of the present invention and water-soluble total reducing sugar, wherein, in turn include the following steps:
(1) deionized water in the liquid to be detected and second container bottle mixes by first threeway, and mixed liquor injects online heating and clears up module, and the temperature that module is cleared up in online heating is 80-120 ℃;
(2) liquid and the deionized water with step (1) gained mixes in second knitting reactor by second threeway;
(3) liquid of step (2) step gained and concentration are that the sodium hydroxide solution of 0.1～1.5mol/L mixes by the 3rd threeway, after mixing and colour developing liquid by the common Input Online heating of the 4th threeway colour developing module, online heating colour developing module temperature is 60～100 ℃;
(4) mixed liquor and the catalyzer with step (3) step gained mixes in second knitting reactor by the 5th threeway;
(5) mixed liquor with step (4) step gained enters optical flow cell, produces spectrogram and is surveyed and drawn, and waste liquid enters waste liquid bottle through back pressure regulator;
(6) sample spectrogram that step (6) is drawn and standard specimen spectrogram relatively calculate the concentration of reducing sugar in the sample.
The method of detection reducing sugar of the present invention, wherein, current-carrying solution is deionized water; Para hydroxybenzene formylhydrazine concentration is 25～100g/L in the chromogenic reagent solution, and citric acid concentration is 10～50g/L, and concentration of hydrochloric acid solution is 0.3～1.0mol/L; Described catalyzer is that concentration is 0.002～0.05mol/L calcium salt soln, cadmium salt soln or bismuth salt solusion, and the light path of flow cell is 10-50mm, and the detection wavelength of detecting device is 370～450nm.
The method of detection reducing sugar of the present invention, wherein, described calcium salt is a lime chloride.
Use the automatic analyzer of any reducing sugar of the present invention and water-soluble total reducing sugar to detect the method for water-soluble total reducing sugar, wherein, in turn include the following steps:
(1) hydrochloric acid in the liquid to be detected and second container bottle mixes by first threeway, and mixed liquor injects online heating and clears up module, and the temperature that module is cleared up in online heating is 80～120 ℃;
(2) with liquid and 0.5～2.5mol/L sodium hydroxide solution of step (1) gained, in second knitting reactor, mix by second threeway;
(3) liquid of step (2) step gained and concentration are that the sodium hydroxide solution of 0.1～1.5mol/L mixes by the 3rd threeway, after mixing and colour developing liquid by the common Input Online heating of the 4th threeway colour developing module, online heating colour developing module temperature is 60～100 ℃;
(4) mixed liquor and the catalyzer with step (3) step gained mixes in second knitting reactor by the 5th threeway;
(5) mixed liquor with step (4) step gained enters optical flow cell, produces spectrogram and is surveyed and drawn, and waste liquid enters waste liquid bottle through back pressure regulator;
(6) sample spectrogram that step (5) is drawn and standard specimen spectrogram relatively calculate the concentration of water-soluble total reducing sugar in the sample.
The method of the water-soluble total reducing sugar of detection of the present invention, wherein, current-carrying solution is deionized water; Described concentration of hydrochloric acid solution is 0.5-2mol/L; Para hydroxybenzene formylhydrazine concentration is 25～100g/L in the chromogenic reagent solution, and citric acid concentration is 10～50g/L, and concentration of hydrochloric acid solution is 0.5～2.0mol/L; Described catalyzer is that concentration is 0.002～0.05mol/L calcium salt soln, cadmium salt soln, bismuth salt solusion, and the light path of flow cell is 10-50mm, and the detection wavelength of detecting device is 370～450nm.
The invention has the beneficial effects as follows:
1, use this kind method and Analysis of Matching instrument thereof, compare with domestic existing manual operations, the automaticity height, can be easily and quickly to the online automatic detection of carrying out of reducing sugar in the water sample and water-soluble total sugar content.
2, the present invention replaces the Continuous Flow Analysis method based on standard method (YC/T 159-2002) with the flow injection method, and instrument is simple, has saved chemical reagent, has avoided the infringement of toxic reagent to operating personnel.
3, optimized differential responses stage knitting reactor length capillaceous and caliber by test of many times, made its response obtain maximal value.
Description of drawings
Fig. 1 is the structural representation of mensuration reducing sugar of the present invention and water-soluble total reducing sugar automatic analyzer.
Embodiment
Be described further below in conjunction with the automatic analyzer of Figure of description reducing sugar of the present invention and water-soluble total reducing sugar.
Present embodiment 1
Referring to Fig. 1, the automatic analyzer of mensuration reducing sugar of the present invention and water-soluble total sugar content comprises sampling device and color developing detection device; Sampling device comprises automatic sampler 1, first peristaltic pump 11, first peristaltic pump 11 is provided with first pump line 12, the sample introduction needle 2 of automatic sampler 1 is connected with the F port of multiport valve 20 by first pump line 12, and the A port of multiport valve 20 is connected with waste liquid tank 36, between be provided with first back pressure regulator 21; The color developing detection device, comprise second peristaltic pump 4, second peristaltic pump 4 is provided with second pump line 13, the 3rd pump line 14, the 4th pump line 15, the 5th pump line 16, the 6th pump line 17, the 7th pump line 18, the two ends of sampling ring 19 are connected the B port and the E port of multiport valve 20, the D port of multiport valve 20 is connected by second pump line 13 with current-carrying solution bottle 5, the C port of multiport valve 20, first threeway 22, module 23 is cleared up in online heating, second threeway 24,25, the three threeways 26 of first knitting reactor, the 4th threeway 28, online heating colour developing module 27, the 5th threeway 29, second knitting reactor 30, flow cell 32, second back pressure regulator 31, waste liquid tank 36 connects successively by connecting capillary; Detecting device 33, data stube cable 35 and computer processing system 34 are connected successively; First threeway 22 links to each other with second container bottle 6 by the 3rd pump line 14, second threeway 24 links to each other with the 3rd container bottle 7 by the 4th pump line 15, the 3rd threeway 26 links to each other with the 4th container bottle 8 by the 5th pump line 16, the 4th threeway 28 links to each other with the 5th container bottle 9 by the 6th pump line 17, and the 5th threeway 29 links to each other with the 6th container bottle 10 by the 7th pump line 18.First to the 7th pump line internal diameter is 0.38～1.85mm, first peristaltic pump, the second peristaltic pump pump speed are 15～60 rev/mins, the sampling ring capillary pipe length is 1～4m, the first knitting reactor capillary pipe length is 0.5～3m, the second knitting reactor capillary pipe length is 1～4m, it is 5～10m that the module capillary pipe length is cleared up in online heating, and online heating colour developing module capillary pipe length is 4～9m, and the internal diameter of connecting capillary is 0.5～1.0mm.
The course of work of automatic analyzer that the present invention measures reducing sugar and water-soluble total sugar content is as follows:
Referring to Fig. 1, be placed in the sample hose 3 through the sample liquid to be measured after the pre-treatment, by automatic sampler 1 under the effect of first peristaltic pump 11, sample enters in the sampling ring 19 that connects on the six-way valve 20 by sample introduction needle 2, after sampling ring 19 was full of, excessive part directly entered waste liquid bottle 36 through first back pressure regulator 21.After sampling process finishes, six-way valve 20 turns to the state shown in the dotted line among the figure, current-carrying in first container bottle 5 is under the effect of second peristaltic pump 4, sample in the sampling ring 19 released by first threeway 22 mix with deionized water in second container bottle 6, enter online heating afterwards and clear up module 23, the mixed solution of heating after clearing up mixes in first knitting reactor 25 by deionized water in second threeway 24 and the 3rd container bottle 7, solution after mixing mixes by the sodium hydroxide solution of the 0.1mol/L in the 3rd threeway 26 and the 4th container bottle 8, mix with the 5th container bottle 9 chromogenic reagent solutions by the 4th threeway 28 more afterwards, enter online heating colour developing module 27 after mixing, the solution that comes out from heating display device 27 mixes second knitting reactor 30 with calcium chloride solution 10 by the 5th threeway 29, pond 32 directly circulates after the mixing, carry out colorimetric estimation by detecting device 33, the liquid that flows out directly enters waste liquid bottle 36 through second back pressure regulator 31.
The course of work of automatic analyzer that the present invention measures reducing sugar and water-soluble total sugar content is as follows:
Referring to Fig. 1, be placed in the sample hose 3 through the sample liquid to be measured after the pre-treatment, by automatic sampler 1 under the effect of first peristaltic pump 11, sample enters in the sampling ring 19 that connects on the six-way valve 20 by sample introduction needle 2, after sampling ring 19 was full of, excessive part directly entered waste liquid bottle 36 through first back pressure regulator 21.After sampling process finishes, six-way valve 20 turns to the state shown in the dotted line among the figure, current-carrying in first container bottle 5 is under the effect of second peristaltic pump 4, sample in the sampling ring 19 is released by the mixed in hydrochloric acid in first threeway 22 and second container bottle 6, enter online heating afterwards and clear up module 23, the mixed solution of heating after clearing up mixes in first knitting reactor 25 by the sodium hydroxide solution of 0.5mol/L in second threeway 24 and the 3rd container bottle 7, solution after mixing mixes by the sodium hydroxide solution of the 0.1mol/L in the 3rd threeway 26 and the 4th container bottle 8, mix with the 5th container bottle 9 chromogenic reagent solutions by the 4th threeway 28 more afterwards, enter online heating colour developing module 27 after mixing, the solution that comes out from heating display device 27 mixes second knitting reactor 30 with calcium chloride solution 10 by the 5th threeway 29, pond 32 directly circulates after the mixing, carry out colorimetric estimation by detecting device 33, the liquid that flows out directly enters waste liquid bottle 36 through second back pressure regulator 31.
Use the automatic analyzer of reducing sugar and water-soluble total reducing sugar, first to the 7th pump pump line internal diameter is 0.38mm, first peristaltic pump, the second peristaltic pump pump speed are 15 rev/mins, described sampling ring capillary pipe length is 1m, the first knitting reactor capillary pipe length is 0.5m, and the second knitting reactor capillary pipe length is 1m, and it is 5m that the module capillary pipe length is cleared up in online heating, online heating colour developing module capillary pipe length is 4m, and the internal diameter of connecting capillary is 0.5mm.Measure the method for reducing sugar, in turn include the following steps:
(1) deionized water in the liquid to be detected and second container bottle 6 mixes by first threeway, and mixed liquor injects online heating and clears up module 23, and the temperature that module is cleared up in online heating is 80 ℃;
(2) liquid and the deionized water with step (1) gained mixes in second knitting reactor 25 by second threeway;
(3) liquid of step (2) step gained and concentration are that the sodium hydroxide solution of 0.1mol/L mixes by the 3rd threeway, after mixing and colour developing liquid heat the module 27 that develops the color by the common Input Online of the 4th threeway, the online heating module temperature 27 that develops the color is 60 ℃;
(4) mixed liquor and the catalyzer with step (3) step gained mixes in second knitting reactor 30 by the 5th threeway;
(5) mixed liquor with step (4) step gained enters optical flow cell 32, produces spectrogram and is surveyed and drawn, and waste liquid enters waste liquid bottle 36 through back pressure regulator 31;
(6) sample spectrogram that step (5) is drawn and standard specimen spectrogram relatively calculate the concentration of reducing sugar in the sample.
Current-carrying solution is deionized water; Para hydroxybenzene formylhydrazine concentration is 25g/L in the chromogenic reagent solution, and citric acid concentration is 10g/L, and concentration of hydrochloric acid solution is 0.3mol/L; Catalyzer is that concentration is the 0.002mol/L calcium chloride solution, and the light path of flow cell is 10mm, and the detection wavelength of detecting device is 370nm.
Table 1 adopts the method for present embodiment 2 and the instrument test result to standard sample:
From this embodiment as can be known, it is accurate to adopt this method to record the result.
Present embodiment 3
The automatic analyzer of reducing sugar and water-soluble total reducing sugar, described first to the 7th pump pump line internal diameter is 1.85mm, described first peristaltic pump, the second peristaltic pump pump speed are 60 rev/mins, described sampling ring capillary pipe length is 4m, the first knitting reactor capillary pipe length is 3m, and the second knitting reactor capillary pipe length is 4m, and it is 10m that the module capillary pipe length is cleared up in online heating, online heating colour developing module capillary pipe length is 9m, and the internal diameter of connecting capillary is 1.0mm.Measure the method for reducing sugar, in turn include the following steps:
(1) deionized water in liquid to be detected and the container bottle (6) mixes by first threeway, and mixed liquor injects online heating and clears up module (23), and the temperature that module is cleared up in online heating is 120 ℃;
(2) liquid and the deionized water with step (1) gained mixes in second knitting reactor 25 by second threeway;
(3) liquid of step (2) step gained and concentration are that the sodium hydroxide solution of 1.5mol/L mixes by the 3rd threeway, after mixing and colour developing liquid heat the module 27 that develops the color by the common Input Online of the 4th threeway, the online heating module temperature 27 that develops the color is 100 ℃;
(4) mixed liquor and the catalyzer with step (3) step gained mixes in second knitting reactor 30 by the 5th threeway;
(5) mixed liquor with step (4) step gained enters optical flow cell 32, produces spectrogram and is surveyed and drawn, and waste liquid enters waste liquid bottle 36 through back pressure regulator 31;
(6) sample spectrogram that step (5) is drawn and standard specimen spectrogram relatively calculate the concentration of reducing sugar in the sample.
Current-carrying solution is deionized water; Para hydroxybenzene formylhydrazine concentration is 100g/L in the chromogenic reagent solution, and citric acid concentration is 50g/L, and concentration of hydrochloric acid solution is 1.0mol/L; Catalyzer is that concentration is that the light path of 0.05mol/L calcium chloride solution flow cell is 50mm, and the detection wavelength of detecting device is 450nm.
Table 2 adopts the method for present embodiment 3 and the instrument test result to standard sample:
From this embodiment as can be known, it is accurate to adopt this method to record the result.
Present embodiment 4
The automatic analyzer of reducing sugar and water-soluble total reducing sugar, described first to the 7th pump pump line internal diameter is 1.1mm, described first peristaltic pump, the second peristaltic pump pump speed are 40 rev/mins, described sampling ring capillary pipe length is 3m, the first knitting reactor capillary pipe length is 2m, and the second knitting reactor capillary pipe length is 2m, and it is 7m that the module capillary pipe length is cleared up in online heating, online heating colour developing module capillary pipe length is 6m, and the internal diameter of connecting capillary is 0.7mm.Measure the method for reducing sugar, in turn include the following steps:
(1) deionized water in liquid to be detected and the container bottle (6) mixes by first threeway, and mixed liquor injects online heating and clears up module (23), and the temperature that module is cleared up in online heating is 100 ℃;
(2) liquid and the deionized water with step (1) gained mixes in second knitting reactor 25 by second threeway;
(3) liquid of step (2) step gained and concentration are that the sodium hydroxide solution of 1.2mol/L mixes by the 3rd threeway, after mixing and colour developing liquid heat the module 27 that develops the color by the common Input Online of the 4th threeway, the online heating module temperature 27 that develops the color is 80 ℃;
(4) mixed liquor and the catalyzer with step (3) step gained mixes in second knitting reactor 30 by the 5th threeway;
(5) mixed liquor with step (4) step gained enters optical flow cell 32, produces spectrogram and is surveyed and drawn, and waste liquid enters waste liquid bottle 36 through back pressure regulator 31;
(6) sample spectrogram that step (5) is drawn and standard specimen spectrogram relatively calculate the concentration of reducing sugar in the sample.
Current-carrying solution is deionized water; Para hydroxybenzene formylhydrazine concentration is 60g/L in the chromogenic reagent solution, and citric acid concentration is 30g/L, and concentration of hydrochloric acid solution is 0.6mol/L; Catalyzer is that concentration is the 0.026mol/L cadmium chloride solution, and the light path of flow cell is 30mm, and the detection wavelength of detecting device is 410nm.
Table 3 adopts the method for present embodiment 4 and the instrument test result to standard sample:
From this embodiment as can be known, it is accurate to adopt this method to record the result.
Use the automatic analyzer of reducing sugar and water-soluble total reducing sugar, first to the 7th pump pump line internal diameter is 0.38mm, described first peristaltic pump, the second peristaltic pump pump speed are 15 rev/mins, described sampling ring capillary pipe length is 1m, the first knitting reactor capillary pipe length is 0.5m, and the second knitting reactor capillary pipe length is 1m, and it is 5m that the module capillary pipe length is cleared up in online heating, online heating colour developing module capillary pipe length is 4m, and the internal diameter of connecting capillary is 0.5mm.The method of measuring water-soluble total reducing sugar in turn includes the following steps:
(1) the salt sour water in liquid to be detected and the container bottle 6 mixes by first threeway, and mixed liquor injects online heating and clears up module 23, and the temperature that module is cleared up in online heating is 80 ℃;
(2) liquid of step (1) gained and the NaOH of 0.5mol/L are mixed in second knitting reactor 25 by second threeway;
(3) liquid of step (2) step gained and concentration are that the sodium hydroxide solution of 0.1mol/L mixes by the 3rd threeway, after mixing and colour developing liquid heat the module 27 that develops the color by the common Input Online of the 4th threeway, the online heating module temperature 27 that develops the color is 60 ℃;
(4) mixed liquor and the catalyzer with step (3) step gained mixes in second knitting reactor 30 by the 5th threeway;
(5) mixed liquor with step (4) step gained enters optical flow cell 32, produces spectrogram and is surveyed and drawn, and waste liquid enters waste liquid bottle 36 through back pressure regulator 31;
(6) sample spectrogram that step (5) is drawn and standard specimen spectrogram relatively calculate the concentration of reducing sugar in the sample.Current-carrying solution is deionized water; Concentration of hydrochloric acid solution is 0.5mol/L; Para hydroxybenzene formylhydrazine concentration is 25g/L in the chromogenic reagent solution, and citric acid concentration is 10g/L, and concentration of hydrochloric acid solution is 0.3mol/L, and catalyzer is that concentration is the 0.002mol/L calcium chloride solution, and the light path of flow cell is 10mm, and the detection wavelength of detecting device is 370nm.
Table 4 adopts the method for present embodiment 5 and the instrument test result to standard sample:
From this embodiment as can be known, it is accurate to adopt this method to record the result.
Present embodiment 6
The automatic analyzer of reducing sugar and water-soluble total reducing sugar, described first to the 7th pump pump line internal diameter is 1.85mm, described first peristaltic pump, the second peristaltic pump pump speed are 60 rev/mins, described sampling ring capillary pipe length is 4m, the first knitting reactor capillary pipe length is 3m, and the second knitting reactor capillary pipe length is 4m, and it is 10m that the module capillary pipe length is cleared up in online heating, online heating colour developing module capillary pipe length is 9m, and the internal diameter of connecting capillary is 1.0mm.Measure the method for water-soluble total reducing sugar, in turn include the following steps:
(1) the salt sour water in liquid to be detected and the container bottle 6 mixes by first threeway, and mixed liquor injects online heating and clears up module 23, and the temperature that module is cleared up in online heating is 80 ℃;
(2) liquid of step (1) gained and the NaOH of 1.5mol/L are mixed in second knitting reactor 25 by second threeway;
(3) liquid of step (2) step gained and concentration are that the sodium hydroxide solution of 1.5mol/L mixes by the 3rd threeway, after mixing and colour developing liquid heat the module 27 that develops the color by the common Input Online of the 4th threeway, the online heating module temperature 27 that develops the color is 100 ℃;
(4) mixed liquor and the catalyzer with step (3) step gained mixes in second knitting reactor 30 by the 5th threeway;
(5) mixed liquor with step (4) step gained enters optical flow cell 32, produces spectrogram and is surveyed and drawn, and waste liquid enters waste liquid bottle 36 through back pressure regulator 31;
(6) sample spectrogram that step (5) is drawn and standard specimen spectrogram relatively calculate the concentration of reducing sugar in the sample.Current-carrying solution is deionized water; Concentration of hydrochloric acid solution is 2mol/L; Para hydroxybenzene formylhydrazine concentration is 100g/L in the chromogenic reagent solution, and citric acid concentration is 50g/L, and concentration of hydrochloric acid solution is 1.0mol/L; Catalyzer is that concentration is that the light path of 0.05mol/L cadmium chloride solution flow cell is 50mm, and the detection wavelength of detecting device is 450nm.
Table 5 adopts the method for present embodiment 6 and the instrument test result to standard sample:
From this embodiment as can be known, it is accurate to adopt this method to record the result.
Present embodiment 7
The automatic analyzer of reducing sugar and water-soluble total reducing sugar, described first to the 7th pump pump line internal diameter is 1.1mm, described first peristaltic pump, the second peristaltic pump pump speed are 40 rev/mins, described sampling ring capillary pipe length is 3m, the first knitting reactor capillary pipe length is 2m, and the second knitting reactor capillary pipe length is 2m, and it is 7m that the module capillary pipe length is cleared up in online heating, online heating colour developing module capillary pipe length is 6m, and the internal diameter of connecting capillary is 0.7mm.Measure the method for water-soluble total reducing sugar, in turn include the following steps:
(1) the salt sour water in liquid to be detected and the container bottle (6) mixes by first threeway, and mixed liquor injects online heating and clears up module 23, and the temperature that module is cleared up in online heating is 80 ℃;
(2) liquid of step (1) gained and the NaOH of 1.5mol/L are mixed in second knitting reactor 25 by second threeway;
(3) liquid of step (2) step gained and concentration are that the sodium hydroxide solution of 1.2mol/L mixes by the 3rd threeway, after mixing and colour developing liquid heat the module 27 that develops the color by the common Input Online of the 4th threeway, the online heating module temperature 27 that develops the color is 80 ℃;
(4) mixed liquor and the catalyzer with step (3) step gained mixes in second knitting reactor 30 by the 5th threeway;
(5) mixed liquor with step (4) step gained enters optical flow cell 32, produces spectrogram and is surveyed and drawn, and waste liquid enters waste liquid bottle 36 through back pressure regulator 31;
(6) sample spectrogram that step (5) is drawn and standard specimen spectrogram relatively calculate the concentration of reducing sugar in the sample.Current-carrying solution is deionized water; Concentration of hydrochloric acid solution is 1.2mol/L; Para hydroxybenzene formylhydrazine concentration is 60g/L in the chromogenic reagent solution, and citric acid concentration is 30g/L, and concentration of hydrochloric acid solution is 0.6mol/L; Catalyzer is that concentration is 0.026mol/L bismuth chloride solution, and the light path of flow cell is 30mm, and the detection wavelength of detecting device is 410nm.
Table 6 adopts the method for present embodiment 7 and the instrument test result to standard sample:
From this embodiment as can be known, it is accurate to adopt this method to record the result.
Above embodiment is described preferred implementation of the present invention; be not that scope of the present invention is limited; design under the prerequisite of spirit not breaking away from the present invention; various distortion and improvement that those of ordinary skills make technical scheme of the present invention all should fall in the definite protection domain of claims of the present invention.
Claims (10)
1. an automatic analyzer of measuring reducing sugar and water-soluble total sugar content is characterized in that, comprises sampling device and color developing detection device; Described sampling device comprises automatic sampler (1), first peristaltic pump (11), described first peristaltic pump (11) is provided with first pump line (12), the sample introduction needle (2) of described automatic sampler (1) is connected with the F port of multiport valve (20) by first pump line (12), the A port of multiport valve (20) is connected with waste liquid tank (36), between be provided with first back pressure regulator (21); Described color developing detection device, comprise second peristaltic pump (4), described second peristaltic pump (4) is provided with second pump line (13), the 3rd pump line (14), the 4th pump line (15), the 5th pump line (16), the 6th pump line (17), the 7th pump line (18), the two ends of sampling ring (19) are connected the B port and the E port of described multiport valve (20), the D port of described multiport valve (20) is connected by described second pump line (13) with current-carrying solution bottle (5), the C port of described multiport valve (20), first threeway (22), module (23) is cleared up in online heating, second threeway (24), first knitting reactor (25), the 3rd threeway (26), the 4th threeway (28), online heating colour developing module (27), the 5th threeway (29), second knitting reactor (20), flow cell (32), second back pressure regulator (31), waste liquid tank (36) connects successively by connecting capillary; Detecting device (33), data stube cable (35) and computer processing system (34) are connected successively; Described first threeway (22) links to each other with second container bottle (6) by the 3rd pump line (14), described second threeway (24) links to each other with the 3rd container bottle (7) by described the 4th pump line (15), described the 3rd threeway (26) links to each other with the 4th container bottle (8) by described the 5th pump line (16), described the 4th threeway (28) links to each other with the 5th container bottle (9) by described the 6th pump line (17), and described the 5th threeway (29) links to each other with the 6th container bottle (10) by described the 7th pump line (18).
2. the automatic analyzer of reducing sugar according to claim 1 and water-soluble total reducing sugar is characterized in that, described first to the 7th pump line internal diameter is 0.38～1.85mm.
3. the automatic analyzer of reducing sugar according to claim 2 and water-soluble total reducing sugar is characterized in that, described first peristaltic pump, the second peristaltic pump pump speed are 15～60 rev/mins.
4. the automatic analyzer of reducing sugar according to claim 3 and water-soluble total reducing sugar is characterized in that, described sampling ring capillary pipe length is 1～4m; The first knitting reactor capillary pipe length is 0.5～3m; The second knitting reactor capillary pipe length is 1～4m; It is 5～10m that the module capillary pipe length is cleared up in online heating; Online heating colour developing module capillary pipe length is 4～9m.
5. the automatic analyzer of reducing sugar according to claim 4 and water-soluble total reducing sugar is characterized in that, the internal diameter of connecting capillary is 0.5～1.0mm.
6. use the method for the automatic analyzer detection reducing sugar of described any reducing sugar of claim 1 to 5 and water-soluble total reducing sugar, it is characterized in that, in turn include the following steps:
(1) deionized water in liquid to be detected and second container bottle (6) mixes by first threeway, and mixed liquor injects online heating and clears up module (23), and the temperature that module is cleared up in online heating is 80-120 ℃;
(2) liquid and the deionized water with step (1) gained mixes in second knitting reactor (25) by second threeway;
(3) liquid of step (2) step gained and concentration are that the sodium hydroxide solution of 0.1～1.5mol/L mixes by the 3rd threeway, after mixing and colour developing liquid by the common Input Online heating of the 4th threeway colour developing module (27), online heating colour developing module temperature (27) is 60～100 ℃;
(4) mixed liquor and the catalyzer with step (3) step gained mixes in second knitting reactor (30) by the 5th threeway;
(5) mixed liquor with step (4) step gained enters optical flow cell (32), produces spectrogram and is surveyed and drawn, and waste liquid enters waste liquid bottle (36) through back pressure regulator (31);
(6) sample spectrogram that step (6) is drawn and standard specimen spectrogram relatively calculate the concentration of reducing sugar in the sample.
7. the method for detection reducing sugar according to claim 7 is characterized in that, described current-carrying solution is deionized water; Para hydroxybenzene formylhydrazine concentration is 25～100g/L in the chromogenic reagent solution, and citric acid concentration is 10～50g/L, and concentration of hydrochloric acid solution is 0.3～1.0mol/L; Described catalyzer is that concentration is 0.002～0.05mol/L calcium salt soln, cadmium salt soln or bismuth salt solusion, and the light path of flow cell is 10-50mm, and the detection wavelength of detecting device is 370～450nm.
8. the method for detection reducing sugar according to claim 7 is characterized in that, described calcium salt is a lime chloride.
9. the automatic analyzer of use described any reducing sugar of claim 1 to 5 and water-soluble total reducing sugar detects the method for water-soluble total reducing sugar, it is characterized in that, in turn includes the following steps:
(1) hydrochloric acid in liquid to be detected and second container bottle (6) mixes by first threeway, and mixed liquor injects online heating and clears up module (23), and the temperature that module is cleared up in online heating is 80～120 ℃;
(2) with liquid and 0.5～2.5mol/L sodium hydroxide solution of step (1) gained, in second knitting reactor (25), mix by second threeway;
(3) liquid of step (2) step gained and concentration are that the sodium hydroxide solution of 0.1～1.5mol/L mixes by the 3rd threeway, after mixing and colour developing liquid by the common Input Online heating of the 4th threeway colour developing module (27), online heating colour developing module temperature (27) is 60～100 ℃;
(4) mixed liquor and the catalyzer with step (3) step gained mixes in second knitting reactor (30) by the 5th threeway;
(5) mixed liquor with step (4) step gained enters optical flow cell (32), produces spectrogram and is surveyed and drawn, and waste liquid enters waste liquid bottle (36) through back pressure regulator (31);
(6) sample spectrogram that step (5) is drawn and standard specimen spectrogram relatively calculate the concentration of water-soluble total reducing sugar in the sample.
10. the method for the water-soluble total reducing sugar of detection according to claim 9 is characterized in that, described current-carrying solution is deionized water; Described concentration of hydrochloric acid solution is 0.5-2mol/L; Para hydroxybenzene formylhydrazine concentration is 25～100g/L in the chromogenic reagent solution, and citric acid concentration is 10～50g/L, and concentration of hydrochloric acid solution is 0.5～2.0mol/L; Described catalyzer is that concentration is 0.002～0.05mol/L calcium salt soln, cadmium salt soln, bismuth salt solusion, and the light path of flow cell is 10-50mm, and the detection wavelength of detecting device is 370～450nm.
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| CN201010182115.9A CN102262059B (en) | 2010-05-25 | 2010-05-25 | Automatic analysis meter and methods for reducing sugar content and water-soluble total sugar content |
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| CN201010182115.9A CN102262059B (en) | 2010-05-25 | 2010-05-25 | Automatic analysis meter and methods for reducing sugar content and water-soluble total sugar content |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102494929A (en) * | 2011-12-05 | 2012-06-13 | 中国水产科学研究院黄海水产研究所 | Method for extracting exogenous total sugar in dried sea cucumbers |
| CN102830117A (en) * | 2012-09-18 | 2012-12-19 | 国家海洋局天津海水淡化与综合利用研究所 | Device and method for carrying out continuous flow detection on bicarbonates in water |
| CN103529021A (en) * | 2012-07-03 | 2014-01-22 | 山东省科学院生物研究所 | Quick analyzing method of total sugar |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102494929A (en) * | 2011-12-05 | 2012-06-13 | 中国水产科学研究院黄海水产研究所 | Method for extracting exogenous total sugar in dried sea cucumbers |
| CN102494929B (en) * | 2011-12-05 | 2013-09-18 | 中国水产科学研究院黄海水产研究所 | Method for extracting exogenous total sugar in dried sea cucumbers |
| CN103529021A (en) * | 2012-07-03 | 2014-01-22 | 山东省科学院生物研究所 | Quick analyzing method of total sugar |
| CN102830117A (en) * | 2012-09-18 | 2012-12-19 | 国家海洋局天津海水淡化与综合利用研究所 | Device and method for carrying out continuous flow detection on bicarbonates in water |
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