CN102260235B - Raw material against influenza A virus as well as preparation and coating - Google Patents
Raw material against influenza A virus as well as preparation and coating Download PDFInfo
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- CN102260235B CN102260235B CN 201010199781 CN201010199781A CN102260235B CN 102260235 B CN102260235 B CN 102260235B CN 201010199781 CN201010199781 CN 201010199781 CN 201010199781 A CN201010199781 A CN 201010199781A CN 102260235 B CN102260235 B CN 102260235B
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Abstract
The invention provides a raw material against influenza A virus. The raw material against influenza A virus is characterized by being prepared by the following method of: distilling a raw material of MEG resin; extracting a fraction of 200-220 DEG C so as to obtain a light yellow oily liquid; putting the light yellow oily liquid into a reaction kettle after being cooled; slowly heating the liquid to 70-80 DEG C; preserving the heat and starting to stir the liquid; slowly dropping phosphoric acid or hydrochloric acid to carry out hydrolysis reaction until the reaction material is transparent and not layered; and then obtaining an MEG disinfectant. The invention also discloses a preparation and a coating, which are both prepared by using the raw material against influenza A virus. The raw material against influenza A virus, provided by the invention, is an air or interface disinfectant mainly used for treating influenza A resistant environments; and the preparation against influenza A virus and the coating against influenza A virus can be used together in various interfaces like spaces, walls, ceilings, grounds and the like of large-sized animal farms, hospitals and public places so as to stop cross infection between people and livestock so that the spread of the epidemic situation can be systematically prevented and controlled from a source point of a pathogen to a public environment of human beings.
Description
Technical field
The present invention relates to the sterilization of a kind of space or interface raw material, particularly a kind of anti-influenza A virus raw material; The invention still further relates to the preparation of this anti-influenza A virus, and the coating that uses this anti-influenza A virus raw material to make.
Background technology
The member of Influenza Virus orthomyxoviridae family is the pathogenic agent of influenza (being commonly called as influenza).Influenza virus is minus-stranded rna virus, is divided into first, second, the third three types.Influenza A virus is further divided into several hypotypes according to outer membrane protein hemagglutinin antigen (HA) and neuraminidase (NA).Our known H1N1, H5N1 influenza virus just belong to one of hypotype of influenza A virus.Influenza A virus easily morphs, and new genotype/antigenic type occurs, can escape host's immune protection, and the vaccine in past is to the effect of crowd's unprotect, and the appearance of new strain causes illness outbreak popular again.Influenza virus mainly is to propagate between the crowd by respiratory tract through the spittle, aerosol.Influenza A virus can also infect multiple animals such as fowl, pig, horse except infecting the mankind, have that infectivity is strong, sickness rate is high, popular characteristics such as wide, is the human Pandemic infection disease that can not effectively control so far.Great viruses such as bird flu (H5N1), H1N1 all are to come from the fowl domestic animals to people's propagation and spread.At present, Influenza A H1N1 is eruption and prevalence worldwide, prevents and treats from environment, is of great immediate significance.
Summary of the invention
Technical problem to be solved by this invention is at the deficiencies in the prior art, and a kind of new anti-influenza A virus raw material that raw material is simple, antiviral effect is good is provided.
Another technical problem to be solved by this invention has provided preparation and the anti-influenza A virus purposes thereof that a kind of anti-influenza A virus raw material is as previously discussed made.
Another technical problem to be solved by this invention has provided the interior wall coating that a kind of anti-influenza A virus raw material is as previously discussed made.
Another technical problem to be solved by this invention has provided the plot coating that a kind of anti-influenza A virus raw material is as previously discussed made.
Technical problem to be solved by this invention is to realize by following technical scheme.The present invention is a kind of anti-influenza A virus raw material, it is characterized in that, it adopts following method to make: it is that raw material distills with the MEG resin, extracts 200-220 ℃ of fraction, gets the pale yellow oily liquid body, drop in the reactor cooling back, slowly be warming up to 70-80 ℃, be incubated again and start stirring, slowly drip the reaction that is hydrolyzed of phosphoric acid or hydrochloric acid, do not have layering and be transparence to reaction mass, namely make anti-influenza A virus raw material MEG sterilizing agent.
In the technique scheme, the rotating speed of stirring can be adjusted as required, is preferably 40-60 commentaries on classics/min.
MEG resin of the present invention refers to: number of patent application is 200710026054.5, publication number is CN101168589, name is called the new type resin described in the Chinese patent of " a kind of production technique of new type resin ", it makes through condensation reaction by propenal and ethyl vinyl ether, and its concrete method for making can be referring to the record in the disclosure patent documentation.Now this resin is on sale in source, Lianyungang Ji institute of microbiology.
The invention also discloses a kind of anti-influenza A virus preparation, be characterized in, it is made by water and the described MEG sterilizing agent of above technical scheme mixed dissolution, and the weight percent that the MEG sterilizing agent accounts for preparation is 0.5-3%.Said preparation can be used for space and interface sterilization.
The invention also discloses a kind of aqueous inner wall paint of anti-influenza A virus, be characterized in, it is mixed and made into by the white slurry of water-based and vehicle, and the described MEG sterilizing agent of the above technical scheme of vehicle is as the function base-material;
Each raw material weight proportioning of the white slurry of water-based is:
Water 140-160; Thickening material 5-9;
Ammoniacal liquor 1-2; Dispersion agent 5-7;
Wetting agent 1-2; Defoamer 1-3;
Titanium dioxide 180-220; Kaolin 70-90;
Water-ground limestone 150-190;
Each raw material weight proportioning of vehicle is:
MEG sterilizing agent 40-60; ACRYLIC EMULSION 230-280;
Film coalescence aid 12-17; Propylene glycol 12-17;
Thickening material 8-12; Defoamer 1-3
Sanitas 1-3; Water 30-50;
Wherein, water-based starch in vain and vehicle between by weight 5-7: 10 allocate.
In the aqueous inner wall paint of above-described anti-influenza A virus, each raw material weight proportion optimization of the white slurry of water-based is:
Water 150; Thickening material 7;
Ammoniacal liquor 1.5; Dispersion agent 6;
Wetting agent 1.5; Defoamer 2;
Titanium dioxide 200; Kaolin 80;
Water-ground limestone 170;
Each raw material weight proportion optimization of vehicle is:
MEG sterilizing agent 50; ACRYLIC EMULSION 250;
Film coalescence aid 15; Propylene glycol 15;
Thickening material 10; Defoamer 2;
Sanitas 2; Water 38;
Wherein, water-based starch in vain and vehicle between allocate by weight preferred 6: 10.
The invention also discloses a kind of water-based Floor paint of anti-influenza A virus, be characterized in, it is mixed and made into by the white slurry of water-based and vehicle, and the described MEG sterilizing agent of the above technical scheme of vehicle is as the function base-material;
Each raw material weight proportioning of the white slurry of water-based is:
MEG sterilizing agent 45-55; Water 110-130;
Natvosol 3-4; Sanitas 2-3;
Propylene glycol 30-40; Dispersion agent 8-10;
Nonionogenic tenside 1.5-2.5; Defoamer 1.5-2.5;
Titanium dioxide 180-220;
Each raw material weight proportioning of vehicle is:
Water 35-45; ACRYLIC EMULSION 480-530;
Butyl carbitol 18-22;
Sanitas 8-10; Defoamer 2-3;
Wherein, water-based starch in vain and vehicle between by weight 4-5: 10 allocate.
In the water-based Floor paint of above-described a kind of anti-influenza A virus,
Each raw material weight proportion optimization of the white slurry of water-based is:
MEG sterilizing agent 50; Water 121;
Natvosol 3.5; Sanitas 2.5;
Propylene glycol 34.3; Dispersion agent 9.1;
Nonionogenic tenside 2.1; Defoamer 1.8;
Titanium dioxide 198;
Each raw material weight proportion optimization of vehicle is:
Water 42; ACRYLIC EMULSION 503;
Butyl carbitol 20.4;
Sanitas 9.5; Defoamer 2.6;
Wherein, water-based starch in vain and vehicle between allocate by weight preferred 4.2: 10.
In interior wall coating of the present invention, the Floor paint, play most critical action function raw material MEG sterilizing agent, therefore, the available concrete kind of each raw material of described in the formulation for coating material other is a lot, therefore if no special instructions, all can refer to disclosed any raw material or marketable material applicable to interior wall coating, Floor paint production in the prior art.
Interior wall coating of the present invention, Floor paint all can prepare by following production technique: successively described each raw material is added in stainless steel vessel under stirring at low speed, fully mix the back high speed dispersion, make fineness reach 50 μ m, make water-based and starch in vain; Each raw material with vehicle fully mixes under stirring at low speed again, adds scattered water-based then and starches in vain, and mixing sieves is finished product.
In the raw material of above-described interior wall or Floor paint, if no special instructions, each raw material all can refer to disclosed any raw material or marketable material applicable to coating material production in the prior art.
The key technical indexes of interior wall coating of the present invention, Floor paint is:
About Stormer viscosity/ku:17.7; About fineness/μ m:50;
Density/(kg/L): 1.45-1.60; About PVC/%:55;
About inactivation of virus rate/%:80%.
The constructional method of interior wall coating of the present invention or Floor paint is: substrate must be handled cleaning, level and smooth, spray, brush, Tu Junke.Easy construction, quick-drying can be brushed 3 times in one day, can select light, inferior light and half inferior light hard floor paint according to on-the-spot needs.
Compared with prior art, anti-influenza A virus raw material of the present invention, preparation and coating have the following advantages:
The one, anti-influenza A virus raw material of the present invention is after anti-influenza A H 1 N 1 virus " vaccine ", can effectively control the product of epidemic situation, this anti-influenza A virus raw material is a kind of air or interface sterilizing agent, is mainly used in the improvement of anti-first stream environment, and does not lie in human body itself.
The 2nd, product anti-influenza A virus preparation of the present invention, anti-influenza A virus coating, can be used in the space of large-scale stock-farms, hospital and public place and each interface such as wall, ceiling, ground, cross infection between the blocking-up people and animals, PE that can be from the source point of pathogenic agent to the mankind systematically prevents and controls its spread in china of epidemic situation.
The 3rd, product of the present invention is the derived product that belongs to the MEG resin, not only has antiviral characteristic, also possesses sterilization, mildew-resistant simultaneously and except the characteristic of algae, wide spectrum, efficient, durable, safety belong to the environment-friendly antibiosis material.
Description of drawings
Fig. 1 is for being tried the anti-first type of materials A H
1N
1The influenza virus action diagram;
Fig. 2 is for being tried the anti-first type of material B H
1N
1The influenza virus action diagram;
Fig. 3 is for being tried the anti-first type of material C H
1N
1The influenza virus action diagram.
Embodiment
Below further describe concrete technical scheme of the present invention, so that those skilled in the art understands the present invention further, and do not constitute the restriction to its right.
Embodiment 1.A kind of anti-influenza A virus raw material, it adopts following method to make: it is that raw material distills with the MEG resin, extract 200 ℃ of fractions, get the pale yellow oily liquid body, drop in the reactor cooling back, slowly is warming up to 70 ℃, be incubated again and start stirring, slowly drip the reaction that is hydrolyzed of phosphoric acid or hydrochloric acid, do not have layering and be transparence to reaction mass, namely make anti-influenza A virus raw material MEG sterilizing agent.
Embodiment 2.A kind of anti-influenza A virus raw material, it adopts following method to make: it is that raw material distills with the MEG resin, extract 220 ℃ of fractions, get the pale yellow oily liquid body, drop in the reactor cooling back, slowly be warming up to 80 ℃, be incubated and start stirring, mixing speed is 60 commentaries on classics/min, slowly drips the reaction that is hydrolyzed of phosphoric acid or hydrochloric acid, do not have layering and be transparence to reaction mass, namely make anti-influenza A virus raw material MEG sterilizing agent.
Embodiment 3.A kind of anti-influenza A virus raw material, it adopts following method to make: it is that raw material distills with the MEG resin, extract 210 ℃ of fractions, get the pale yellow oily liquid body, drop in the reactor cooling back, slowly be warming up to 75 ℃, be incubated and start stirring, mixing speed is 40 commentaries on classics/min, slowly drips the reaction that is hydrolyzed of phosphoric acid or hydrochloric acid, do not have layering and be transparence to reaction mass, namely make anti-influenza A virus raw material MEG sterilizing agent.
Embodiment 4.A kind of anti-influenza A virus preparation, it is made by any one described MEG sterilizing agent mixed dissolution among water and the embodiment 1-3, and the weight percent that the MEG sterilizing agent accounts for preparation is 0.5%.
Embodiment 5.A kind of anti-influenza A virus preparation, it is made by any one described MEG sterilizing agent mixed dissolution among water and the embodiment 1-3, and the weight percent that the MEG sterilizing agent accounts for preparation is 3%.
Embodiment 6.A kind of anti-influenza A virus preparation, it is made by any one described MEG sterilizing agent mixed dissolution among water and the embodiment 1-3, and the weight percent that the MEG sterilizing agent accounts for preparation is 1.5%.
Embodiment 7.A kind of aqueous inner wall paint of anti-influenza A virus, it is mixed and made into the white slurry of water-based and vehicle, vehicle with any one described MEG sterilizing agent among the embodiment 1-3 as the function base-material;
Each raw material weight proportioning of the white slurry of water-based is:
Water 140; Thickening material 5;
Titanium dioxide 180; Kaolin 70;
Water-ground limestone 150;
Each raw material weight proportioning of vehicle is:
MEG sterilizing agent 40; ACRYLIC EMULSION 230;
Film coalescence aid 12; Propylene glycol 12;
Thickening material 8; Defoamer 1;
Wherein, water-based starch in vain and vehicle between by weight carrying out mixing preparation at 5: 10.
Embodiment 8.A kind of aqueous inner wall paint of anti-influenza A virus, it is mixed and made into the white slurry of water-based and vehicle, vehicle with any one described MEG sterilizing agent among the embodiment 1-3 as the function base-material;
Each raw material weight proportioning of the white slurry of water-based is:
Water 160; Thickening material 9;
Titanium dioxide 220; Kaolin 90;
Water-ground limestone 190;
Each raw material weight proportioning of vehicle is:
MEG sterilizing agent 60; ACRYLIC EMULSION 280;
Film coalescence aid 17; Propylene glycol 17;
Thickening material 12; Defoamer 3
Wherein, water-based starch in vain and vehicle between by weight carrying out mixing preparation at 7: 10.
Embodiment 9.A kind of aqueous inner wall paint of anti-influenza A virus, it is mixed and made into the white slurry of water-based and vehicle, vehicle with embodiment 2 described MEG sterilizing agents as the function base-material;
Each raw material weight proportioning of the white slurry of water-based is:
Water 150; Thickening material 7;
Ammoniacal liquor 1.5; Dispersion agent 6;
Wetting agent 1.5; Defoamer 2;
Titanium dioxide 200; Kaolin 80;
Water-ground limestone 170;
Each raw material weight proportioning of vehicle is:
MEG sterilizing agent 50; ACRYLIC EMULSION 250;
Film coalescence aid 15; Propylene glycol 15;
Thickening material 10; Defoamer 2
Wherein, water-based starch in vain and vehicle between allocate by weight 6: 10.
Embodiment 10.A kind of water-based Floor paint of anti-influenza A virus, it is mixed and made into by the white slurry of water-based and vehicle, vehicle with any one described MEG sterilizing agent of embodiment 1-3 as the function base-material;
Each raw material weight proportioning of the white slurry of water-based is:
MEG sterilizing agent 45; Water 110;
Propylene glycol 30; Dispersion agent 8;
Nonionogenic tenside 1.5; Defoamer 1.5;
Titanium dioxide 180;
Each raw material weight proportioning of vehicle is:
Water 35; ACRYLIC EMULSION 480;
Butyl carbitol 18;
Sanitas 8; Defoamer 2;
Wherein, water-based starch in vain and vehicle between allocate by weight 4: 10.
Embodiment 11.A kind of water-based Floor paint of anti-influenza A virus, it is mixed and made into by the white slurry of water-based and vehicle, vehicle with any one described MEG sterilizing agent of embodiment 1-3 as the function base-material;
Each raw material weight proportioning of the white slurry of water-based is:
MEG sterilizing agent 55; Water 130;
Propylene glycol 40; Dispersion agent 10;
Nonionogenic tenside 2.5; Defoamer 2.5;
Titanium dioxide 220;
Each raw material weight proportioning of vehicle is:
Water 45; ACRYLIC EMULSION 530;
Butyl carbitol 22;
Sanitas 10; Defoamer 3;
Wherein, water-based starch in vain and vehicle between allocate by weight 5: 10.
Embodiment 12.A kind of water-based Floor paint of anti-influenza A virus, it is mixed and made into by the white slurry of water-based and vehicle, vehicle with embodiment 2 described MEG sterilizing agents as the function base-material;
Each raw material weight proportioning of the white slurry of water-based is:
MEG sterilizing agent 50; Water 121;
Natvosol 3.5; Sanitas 2.5;
Propylene glycol 34.3; Dispersion agent 9.1;
Nonionogenic tenside 2.1; Defoamer 1.8;
Titanium dioxide 198;
Each raw material weight proportioning of vehicle is:
Water 42; ACRYLIC EMULSION 503;
Butyl carbitol 20.4;
Sanitas 9.5; Defoamer 2.6;
Wherein, water-based starch in vain and vehicle between allocate by weight 4.2: 10.
Experimental example.The different derivatives of MEG resin to first type H
1N
1The effect experiment of influenza virus.This experiment is that the applicant entrusts virusology key lab of Wuhan University virus institute/national to do, and experimental session is year December in July, 2009-2009.
1, experiment purpose
(1) being tried material tests the toxic action of mdck cell.
(2) first type H
1N
1The influenza virus titer determination.
(3) tried material to the influenza A virus direct killing effect.
2, experimental technique
2.1 tried material and preparation
Tried materials A: embodiment 3 described MEG sterilizing agents, faint yellow aqua;
Tried material B: embodiment 9 described aqueous inner wall paints, light brown aqua;
Tried material C: embodiment 12 described water-based Floor paints, oyster white aqua;
Tried the material compound method: being tried material provides by the lucky institute of microbiology in source, Lianyungang, and keeps substratum with cell and be diluted to corresponding concentration.
2.2 use substratum
1. cell proliferation substratum
DMEM (GIBCO) substratum that contains 10% calf serum (GIBCO).Penicillin, content of streptomycin are 100IUml
-1
2. cell is kept substratum
Except not containing the serum, all the other add trypsin 2 μ gml in addition with the cell proliferation substratum
-1).
2.3 cell and cultivation
Madin-Darby canine kidney(cell line) (MDCK) (MDCK) is preserved for this laboratory.With cell proliferation substratum monolayer culture mdck cell in Tissue Culture Flask.Tissue Culture Flask places 37 ℃, 5%CO
2In the incubator.
2.4 experiment prepares with viral suspension
First type H
1N
1Influenza virus preserves for this laboratory.With viral liquid with 15 μ gml
-1Pancreatin is inoculated in the Madin-Darby canine kidney(cell line) (MDCK) (MDCK) that grows up to individual layer after handling, and discards viral liquid behind the 2h, adds behind the washed cell and contains 2 μ gml
-1The cell maintenance medium of pancreatin, in 37 ℃, 5%CO
2Cultivated in the incubator 1～3 day.Go down to posterity 2～3 times before seed culture of viruses uses with on the MDCK, collect viral liquid packing behind the multigelation 3 times, it is standby that all seeds culture of viruses are all put-80 ℃ of preservations.
2.5 experimental technique
2.5.1 tried material to mdck cell toxicity (TC
50) measure
On 96 orifice plates, every hole adds 0.1mL, 5 * 10
4After the mdck cell of concentration, 24h treat that cell grows up to individual layer, add and to be tried material containing of different concns and keep liquid and continue to cultivate.Every day, observation of cell changed under inverted microscope, and behind the 48h, mtt assay detects cell survival rate.Each is tried material concentration and is all repeated 4 holes.Establish the normal cell contrast simultaneously.Cell survival rate (%)=(experimental group average A value/cell control group average A value) * 100%.Analyze with the data of the SPSS17.0 of statistical software, and calculate the half cytotoxicity concentration (TC50) of being tried material.
2.5.2 first type H
1N
1The influenza virus titer determination
On 96 orifice plates, every hole adds 5 * 10 of 100 μ l
4The mdck cell of concentration.After treating that the 24h cell grows up to individual layer, keep the substratum washing once with cell, add viral liquid (50 μ l/ hole) the absorption 2h by 10 times of titre gradient dilutions again.Change cell behind the sucking-off virus liquid and keep substratum (200 μ l/ hole), put into 37 ℃ of incubators, every day, observation of cell changed under inverted microscope, and behind the 48h, mtt assay detects cell survival rate.Each virus titer all repeats 4 holes, does 10 gradients altogether, establishes the normal cell contrast simultaneously.Cell survival rate (%)=(experimental group average A value/cell control group average A value) * 100%.Analyze with the data of the SPSS17.0 of statistical software, and calculate (the TCID of 50tissue infection dose
50).
2.5.3 tried the anti-first type of material H
1N
1The influenza virus effect
Method one: use 100TCID earlier
50Virus and nontoxic scope in different concns tried material preact 6 hours, every hole 50 μ L cells infecteds behind the absorption 2h, are changed to cell and keep substratum, every day, observation of cell under inverted microscope changed (be applicable to and tried materials A).
Method two: get and tried material in right amount and evenly paint one deck airing, add first type H
1N
1Influenza virus stock solution, 4 ℃ of effects are after 6 hours, and by 10 times of 7 titres of dilution of successively decreasing, every hole 50 μ L cells infecteds behind the absorption 2h, are changed to cell and keep substratum with virus, and every day, observation of cell changed under inverted microscope.(be applicable to and tried material B and tried material C).
Observation index: cytopathic effect (CPE), recording method is :-be no CPE ,+be that CPE appears in 25% cell, ++ be that CPE appears in 50% cell, +++be that CPE appears in 75% cell, ++ ++ be that CPE appears in 100% cell.Treat that virus control hole CPE reaches more than 80% and cell contrasts just often, detect cell survival rate with mtt assay, each is tried materials A concentration and is all repeated 4 holes, establishes normal cell contrast and virus control simultaneously.
3, experimental result
3.1 tried material to the toxic action of mdck cell
Being tried materials A shows as the increase of cell refractivity, cell rounding, OD value to the toxic action of mdck cell and obviously descends.And tried material to the toxic action of cell within the specific limits along with the increase that is tried material concentration, cell survival rate reduces.Measure the OD value by microscope observing cell form and mtt assay, carry out data analysis by SPSS17.0 and calculate former medicine, being tried material B is 1 * 10 with the half cytotoxicity concentration (TC50) of being tried material C
-2(being tried the volume ratio of material and diluent), the half cytotoxicity concentration (TC50) of being tried materials A is about 4 * 10
-4(being tried the volume ratio of material and diluent).
3.2 first type H
1N
1The influenza virus titre
Influenza A virus shows as cell to mdck cell CPE and becomes that starlike, gathering, refractivity strengthen, come off, the OD value descends.And the CPE degree of virus increases along with the increase of virus titer within the specific limits.Measure the OD value by microscope observing cell form and mtt assay, determine first type H
1N
1Influenza virus TCID
50Be 10
-3.
Table 1 titration of virus result
3.3 tried the anti-first type of material H
1N
1The influenza virus effect
Influenza A virus shows as cell to mdck cell CPE and becomes that starlike, gathering, refractivity strengthen, come off, the OD value descends.Measure the OD value by microscope observing cell form and mtt assay, it is as follows to the biosynthetic restraining effect experimental result of influenza A virus respectively to be tried material.
Table 2 is tried the anti-first type of materials A H
1N
1The influenza virus effect
Table 3 is tried materials A to first type H
1N
1The influenza virus effect
With reference to Fig. 1 (the medicine group described in the figure is the corresponding material group of being tried, Fig. 2, Fig. 3 with), wherein: gradient 1～5th, tried materials A extent of dilution/virus titer and be respectively 4 * 10
-4/ 10
-1, 4 * 10
-5/ 10
-1, 4 * 10
-6/ 10
-1, 4 * 10
-7/ 10
-1, 4 * 10
-8/ 10
-1
Table 4 is tried the anti-first type of material B H
1N
1The influenza virus effect
The anti-first type of material B that tried H with reference to Fig. 2 demonstration
1N
1Influenza virus effect (the same Fig. 1 of gradient 1～7 expression).
Table 5 is tried the effect of material C anti-influenza A H 1 N 1 virus
"? " precipitation is arranged, can't observe.
And the anti-first type of material C that the tried H that shows with reference to Fig. 3
1N
1Influenza virus effect (the same Fig. 1 of gradient 1～7 expression).
Conclusion
This experiment has been carried out anti-first type H to try material based on the difference of MEG sterilizing agent
1N
1Influenza virus experimental study, purpose are to wish from each space and interface, form the deactivation to influenza virus.This experiment with 3 kinds tried materials A, B, C carry out antiviral experiment, the result shows that being tried the materials A extent of dilution is 1 * 10
-8, the inactivation of virus rate is 89.7%.Antivirus action is changed with trying material concentration, is tangible dose-effect relationship.Being tried the material B extent of dilution is 1 * 10
-2, the inactivation of virus rate is 84.8%.Being tried the material C extent of dilution is 1 * 10
-2, the inactivation of virus rate is 88.7%.Conclusion thinks that the MEG sterilizing agent has tangible anti-first type H
1N
1The influenza virus effect.
Claims (8)
1. anti-influenza A virus raw material, it is characterized in that, it adopts following method to make: it is that raw material distills with the MEG resin, extracts 200-220 ℃ of fraction, gets the pale yellow oily liquid body, drop in the reactor cooling back, slowly be warming up to 70-80 ℃, be incubated again and start stirring, slowly drip the reaction that is hydrolyzed of phosphoric acid or hydrochloric acid, do not have layering and be transparence to reaction mass, namely make anti-influenza A virus raw material MEG sterilizing agent; Described MEG resin makes through condensation reaction by propenal and ethyl vinyl ether; The production stage of its condensation reaction is as follows:
(1) ethyl vinyl ether is added the condensation still, then catalyzer zinc salt or aluminium salt are dropped in the still, start stirring, again propenal is dropped into the condensation still, valve-off after feeding intake, simultaneously, the chuck of condensation still heats with hot water, slowly heat up, and the condenser of condensation still top cooled off, solution is refluxed, when temperature rise in the condensation still during to 60-65 ℃, stop heating, be cooled to and material be evacuated in the still pot after being lower than 30 ℃;
(2) material that obtains is carried out air distillation to 230-240 ℃, open the storage tank valve of still kettle top, in still kettle, drop into the blocking-up resin, after the congruent melting, collection is lower than all fractions of 240 ℃, gets byproduct 2-oxyethyl group-3, and 4-dihydropyrane first product also drops into storage tank; With discharging after being retained in superpolymer in the still kettle and being cooled to 200 ℃ naturally, namely.
2. a kind of anti-influenza A virus raw material according to claim 1 is characterized in that: drop in the reactor described pale yellow oily liquid body cooling back, slowly is warming up to 70-80 ℃, is incubated and the rotating speed that starts stirring is 40-60 commentaries on classics/min again.
3. an anti-influenza A virus preparation is characterized in that, it is made by water and claim 1 or 2 described MEG sterilizing agent mixed dissolutions, and the weight percent that the MEG sterilizing agent accounts for preparation is 0.5-3%.
4. claim 1 or the 2 described anti-influenza A virus raw materials purposes in preparation anti-influenza A virus disinfectant preparation.
5. the aqueous inner wall paint of an anti-influenza A virus is characterized in that, it is mixed and made into the white slurry of water-based and vehicle, vehicle with claim 1 or 2 described MEG sterilizing agents as the function base-material;
Each raw material weight proportioning of the white slurry of water-based is:
Each raw material weight proportioning of vehicle is:
Wherein, water-based starch in vain and vehicle between by weight 5-7: 10 carry out mixing preparation.
6. the aqueous inner wall paint of anti-influenza A virus according to claim 5 is characterized in that, each raw material weight proportioning of the white slurry of water-based is:
Each raw material weight proportioning of vehicle is:
Wherein, water-based starch in vain and vehicle between allocate by weight 6: 10.
7. the water-based Floor paint of an anti-influenza A virus is characterized in that, it is mixed and made into by the white slurry of water-based and vehicle, vehicle with claim 1 or 2 described MEG sterilizing agents as the function base-material;
Each raw material weight proportioning of the white slurry of water-based is:
Each raw material weight proportioning of vehicle is:
Water 35-45; ACRYLIC EMULSION 480-530;
Butyl carbitol 18-22;
Sanitas 8-10; Defoamer 2-3;
Wherein, water-based starch in vain and vehicle between by weight 4-5: 10 allocate.
8. the water-based Floor paint of a kind of anti-influenza A virus according to claim 6 is characterized in that,
Each raw material weight proportioning of the white slurry of water-based is:
Each raw material weight proportioning of vehicle is:
Water 42; ACRYLIC EMULSION 503;
Butyl carbitol 20.4;
Sanitas 9.5; Defoamer 2.6;
Wherein, water-based starch in vain and vehicle between allocate by weight 4.2: 10.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006132668A2 (en) * | 2004-11-04 | 2006-12-14 | Alistagen Corporation | Novel uses of calcium hydroxide |
| CN101168589A (en) * | 2007-08-14 | 2008-04-30 | 徐友志 | Technique for producing resin |
| WO2008127416A3 (en) * | 2006-11-08 | 2008-12-11 | Massachusetts Inst Technology | Polymeric coatings that inactivate viruses and bacteria |
| CN101698769A (en) * | 2009-10-19 | 2010-04-28 | 佛山夫田涂料化工有限公司 | Paint for preventing influenza viruses and preparation method thereof |
| CN101724336A (en) * | 2009-12-24 | 2010-06-09 | 北京联飞翔科技股份有限公司 | Coating material and preparation method thereof |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006132668A2 (en) * | 2004-11-04 | 2006-12-14 | Alistagen Corporation | Novel uses of calcium hydroxide |
| WO2008127416A3 (en) * | 2006-11-08 | 2008-12-11 | Massachusetts Inst Technology | Polymeric coatings that inactivate viruses and bacteria |
| CN101168589A (en) * | 2007-08-14 | 2008-04-30 | 徐友志 | Technique for producing resin |
| CN101698769A (en) * | 2009-10-19 | 2010-04-28 | 佛山夫田涂料化工有限公司 | Paint for preventing influenza viruses and preparation method thereof |
| CN101724336A (en) * | 2009-12-24 | 2010-06-09 | 北京联飞翔科技股份有限公司 | Coating material and preparation method thereof |
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