CN102250955A - Fermentation medium for promoting generation of antioxidant - Google Patents
Fermentation medium for promoting generation of antioxidant Download PDFInfo
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- CN102250955A CN102250955A CN201110127680XA CN201110127680A CN102250955A CN 102250955 A CN102250955 A CN 102250955A CN 201110127680X A CN201110127680X A CN 201110127680XA CN 201110127680 A CN201110127680 A CN 201110127680A CN 102250955 A CN102250955 A CN 102250955A
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Abstract
The invention discloses to a fermentation medium for promoting the generation of an antioxidant and relates to a culture medium. The fermentation medium for promoting the generation of an antioxidant is composed of a carbon-based nutrient component(s), a nitrogen-based nutrient component(s), a metal ion(s) and the balance of distilled water, wherein the carbon-based nutrient component(s) can be one or more than one of glucose, sucrose and starch syrup; the nitrogen-based nutrient component can be one or more than one of corn steep liquor, peptone and yeast extract; the metal ion(s) can be one or more than one of manganese ion, magnesium ion and zinc ion. The fermentation medium for promoting the generation of an antioxidant is composed of the carbon-based nutrient component(s), the nitrogen-based nutrient component(s), agar, the metal ion(s) and the balance of distilled water. The fermentation medium for promoting the generation of an antioxidant, which is provided by the invention, is a liquid culture medium or a solid culture medium, the components of the culture medium are easy to obtain and have low cost; the fermentation medium can be used for promoting the synthesis of the antioxidant; each gram of fermented culture contains 100.7-214.4 mg (dry weight) of the antioxidant.
Description
Technical field
The present invention relates to substratum.
Background technology
Free radical is the intermediary metabolism substance of multiple biochemical reaction in the vital movement.Free-radical generating too much or removed slowly can cause the various damages of body in molecular level, cell levels and histoorgan level, and the aging course of acceleration body is also brought out various diseases.In aging course, variation has taken place in the balance in the body between oxidation and the antioxidant system, showing as oxygenizement strengthens, resistance of oxidation weakens with advancing age, antioxidant reductase such as SOD isoreactivity reduce, the ability of removing oxyradical in the body is affected, concentration of oxygen free radicals raises, cause the harm such as peroxidation, protein denaturation, enzymic activity reduction and dna damage of membrane lipid, the change of these biomacromolecule structures and function, must cause the disorder of cell function, thereby cause various diseases and cause aging.Therefore saying that free radical is attacked life macromolecule and various organoid causes tissue injury, is the basic reason that causes body aging, also is the great reason of malignant disease such as induced tumor.
Antioxidant (antioxidant) is meant can remove oxyradical, a class material that suppresses or eliminate and slow down oxidizing reaction.The exploitation of antioxidant gets more and more people's extensive concerning with research all the time, and particularly after scientists found that the disease of human body is the result of free radical effect in the human body with aging, Study on antioxidants had more had wide development space.Natural antioxidants from merely as the antioxidant of grease and fat-containing food, develop into scavenging agent as oxyradical in the body, play the protection human cell tissue, protection cardiovascular and cerebrovascular circulation system, anticancer and physiological action such as delay senility.
The natural anti-oxidation material mainly extracts from animals and plants at present, as vitamins, enzyme and natural drug class etc.But natural anti-oxidation material content is lower in the animals and plants, the extraction process complexity, and extract from these resources these these animal and plant resources are seriously damaged, cause the loss of species diversity and ecotope.In recent years, utilize some microorganisms to have the characteristics of generation and the same or similar physiologically active substance of host, the plant origin bacterium has been carried out a large amount of research, separation obtains many strains and can produce the bacterial strain with physiologically active substance from the Corylus plant, (bacterial strain that produces antioxidant is mould JTLR-1 comprising the mould that can produce antioxidant, classification called after mould Penicillium sp, be deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC), the preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date is on March 21st, 2011, deposit number is CGMCC No.4701), but the nutritional formula that promotes mould to produce antioxidant does not have report yet.
Summary of the invention
The object of the invention provides the fermention medium that short antioxidant generates.
The every L of fermention medium that short antioxidant generates is made up of the carbon source nutritive ingredient of 5～100g, the nitrogenous source nutritive ingredient of 0.1～6g, the metal ion of 0.001～0.04mol and the distilled water of surplus; Wherein the carbon source nutritive ingredient is one or more in glucose, sucrose, the starch syrup; The nitrogenous source nutritive ingredient is one or more in corn steep liquor, peptone, the yeast extract paste; Metal ion is one or more in mn ion, magnesium ion, the zine ion.
The every L of fermention medium that short antioxidant generates is made up of the carbon source nutritive ingredient of 5～100g, the nitrogenous source nutritive ingredient of 0.1～6g, the agar of 1～100g, the metal ion of 0.001～0.04mol and the distilled water of surplus; Wherein the carbon source nutritive ingredient is one or more in glucose, sucrose, the starch syrup; The nitrogenous source nutritive ingredient is one or more in corn steep liquor, peptone, the yeast extract paste; Metal ion is one or more in mn ion, magnesium ion, the zine ion.
The fermention medium that short antioxidant generates among the present invention is liquid nutrient medium or solid medium, nutrient media components is easy to get, with low cost, can promote antioxidant synthetic, every gram fermenting culture (with the dry weight basis that anhydrates) contains antioxidant 100.7mg～214.4mg.
Embodiment
Embodiment one: the every L of fermention medium that the short antioxidant of present embodiment generates is made up of the carbon source nutritive ingredient of 5～100g, the nitrogenous source nutritive ingredient of 0.1～6g, the metal ion of 0.001～0.04mol and the distilled water of surplus; Wherein the carbon source nutritive ingredient is one or more in glucose, sucrose, the starch syrup; The nitrogenous source nutritive ingredient is one or more in corn steep liquor, peptone, the yeast extract paste; Metal ion is one or more in mn ion, magnesium ion, the zine ion.
Press when the carbon source nutritive ingredient is formed for two or more material in the present embodiment arbitrarily than mixing; When forming for two or more material, the nitrogenous source nutritive ingredient presses arbitrarily than mixing; When forming for two or more material, presses metal ion arbitrarily than mixing.
Embodiment two: the every L of fermention medium that is not both short antioxidant generation of present embodiment and embodiment one is made up of the carbon source nutritive ingredient of 5g, the nitrogenous source nutritive ingredient of 0.1g, the metal ion of 0.001mol and the distilled water of surplus.Other is identical with embodiment one.
Embodiment three: the every L of fermention medium that is not both short antioxidant generation of present embodiment and embodiment one is made up of the carbon source nutritive ingredient of 100g, the nitrogenous source nutritive ingredient of 6g, the metal ion of 0.04mol and the distilled water of surplus.Other is identical with embodiment one.
Embodiment four: the every L of fermention medium that is not both short antioxidant generation of present embodiment and embodiment one is made up of the carbon source nutritive ingredient of 10～90g, the nitrogenous source nutritive ingredient of 0.5～5g, the metal ion of 0.002～0.03mol and the distilled water of surplus.Other is identical with embodiment one.
Embodiment five: the every L of fermention medium that is not both short antioxidant generation of present embodiment and embodiment one is made up of the carbon source nutritive ingredient of 30g, the nitrogenous source nutritive ingredient of 2g, the metal ion of 0.005mol and the distilled water of surplus.Other is identical with embodiment one.
Embodiment six: the every L of fermention medium that is not both short antioxidant generation of present embodiment and embodiment one is made up of the carbon source nutritive ingredient of 50g, the nitrogenous source nutritive ingredient of 3g, the metal ion of 0.01mol and the distilled water of surplus.Other is identical with embodiment one.
Embodiment seven: the every L of fermention medium that is not both short antioxidant generation of present embodiment and embodiment one is made up of the carbon source nutritive ingredient of 60g, the nitrogenous source nutritive ingredient of 4g, the metal ion of 0.02mol and the distilled water of surplus.Other is identical with embodiment one.
Embodiment eight: the mn ion that is not both of one of present embodiment and embodiment one to seven derives from MnSO
4H
2O.Other is identical with one of embodiment one to seven.
Embodiment nine: the magnesium ion that is not both of one of present embodiment and embodiment one to eight derives from MgSO
47H
2O.Other is identical with one of embodiment one to eight.
Embodiment ten: the zine ion that is not both of one of present embodiment and embodiment one to nine derives from ZnCl
2Other is identical with one of embodiment one to nine.
Embodiment 11: the every L of fermention medium that the short antioxidant of present embodiment generates is made up of the carbon source nutritive ingredient of 5～100g, the nitrogenous source nutritive ingredient of 0.1～6g, the agar of 1～100g, the metal ion of 0.001～0.04mol and the distilled water of surplus; Wherein the carbon source nutritive ingredient is one or more in glucose, sucrose, the starch syrup; The nitrogenous source nutritive ingredient is one or more in corn steep liquor, peptone, the yeast extract paste; Metal ion is one or more in mn ion, magnesium ion, the zine ion.
Press when the carbon source nutritive ingredient is formed for two or more material in the present embodiment arbitrarily than mixing; When forming for two or more material, the nitrogenous source nutritive ingredient presses arbitrarily than mixing; When forming for two or more material, presses metal ion arbitrarily than mixing.
Embodiment 12: the every L of fermention medium that is not both short antioxidant generation of present embodiment and embodiment 11 is made up of the carbon source nutritive ingredient of 15g, the nitrogenous source nutritive ingredient of 1g, the agar of 10g, the metal ion of 0.03mol and the distilled water of surplus.Other is identical with embodiment 11.
Embodiment 13: the every L of fermention medium that is not both short antioxidant generation of present embodiment and embodiment 11 is made up of the carbon source nutritive ingredient of 20g, the nitrogenous source nutritive ingredient of 2g, the agar of 20g, the metal ion of 0.01mol and the distilled water of surplus.Other is identical with embodiment 11.
Embodiment 14: the every L of fermention medium that is not both short antioxidant generation of present embodiment and embodiment 11 is made up of the carbon source nutritive ingredient of 40g, the nitrogenous source nutritive ingredient of 5g, the agar of 50g, the metal ion of 0.02mol and the distilled water of surplus.Other is identical with embodiment 11.
Embodiment 15: the mn ion that is not both of one of present embodiment and embodiment 11 to 14 derives from MnSO
4H
2O.Other is identical with one of embodiment 11 to 14.
Embodiment 16: the magnesium ion that is not both of one of present embodiment and embodiment 11 to 15 derives from MgSO
47H
2O.Other is identical with one of embodiment 11 to 15.
Embodiment 17: the zine ion that is not both of one of present embodiment and embodiment 11 to 16 derives from ZnCl
2Other is identical with one of embodiment 11 to 16.
Embodiment 18: the every L of fermention medium that the short antioxidant of present embodiment generates is made up of the sucrose of 20g, the yeast extract paste of 2g, the mn ion of 0.01mol, the magnesium ion of 0.01mol, the zine ion of 0.01mol and the distilled water of surplus.
The bacterial strain mould JTLR-1 of antioxidant will be produced, be inoculated in seed culture medium (PDA solid medium, by the 100ml mass concentration is that 20% potato juice, the sucrose of 2g and the agar of 2g are formed), cultivated 2 days at 28 ℃, transfer then in the 500mL triangular flask of the fermention medium that the short antioxidant generation of 200mL is housed, shake-flask culture is 7 days under 28 ℃, 180r/min condition, collects fermenting culture; With fermenting culture with liquid nitrogen freezing and after grinding, the ratio that adds the methanol-water of 10mL mass concentration 50% with the 1g fermenting culture is extracted, use n-hexane extraction, after n-hexane extract is colourless, remove upper strata normal hexane layer, take off a layer lyophilize, promptly get antioxidant, as calculated, every gram fermenting culture (with the dry weight basis that anhydrates) contains antioxidant 164.6mg.
Embodiment 19: the every L of fermention medium that the short antioxidant of present embodiment generates is made up of the sucrose of 15g, the peptone of 1g, the agar of 10g, the mn ion of 0.01mol, the magnesium ion of 0.01mol, the zine ion of 0.01mol and the distilled water of surplus.
With producing the bacterial strain mould JTLR-1 of antioxidant, be inoculated in seed culture medium (PDA solid medium), 28 ℃ of cultivations 3 days, transfer then in the big plate of the fermention medium that the short antioxidant generation of 50mL is housed, cultivate down in 25 ℃ and got culture in 7 days; With culture with liquid nitrogen freezing and after grinding, the ratio that adds the methanol-water of 10mL mass concentration 50% with the 1g culture is extracted, use n-hexane extraction, after n-hexane extract is colourless, remove upper strata normal hexane layer, take off a layer lyophilize, promptly get antioxidant, as calculated, every gram fermenting culture (with the dry weight basis that anhydrates) contains antioxidant 106.2mg.
Embodiment 20: the every L of fermention medium that the short antioxidant of present embodiment generates is made up of the glucose of 20g, the yeast extract paste of 2g, the agar of 10g, the mn ion of 0.02mol, the magnesium ion of 0.01mol, the zine ion of 0.01mol and the distilled water of surplus.
With producing the bacterial strain mould JTLR-1 of antioxidant, be inoculated in seed culture medium (PDA solid medium), 28 ℃ of cultivations 3 days, transfer then in the big plate of the fermention medium that the short antioxidant generation of 50mL is housed, cultivate down in 25 ℃ and got culture in 7 days; With culture with liquid nitrogen freezing and after grinding, the ratio that adds the methanol-water of 10mL mass concentration 50% with the 1g culture is extracted, use n-hexane extraction, after n-hexane extract is colourless, remove upper strata normal hexane layer, take off a layer lyophilize, promptly get antioxidant, as calculated, every gram fermenting culture (with the dry weight basis that anhydrates) contains antioxidant 214.4mg.
Claims (10)
1. the fermention medium that short antioxidant generates is characterized in that the every L of fermention medium that short antioxidant generates is made up of the carbon source nutritive ingredient of 5～100g, the nitrogenous source nutritive ingredient of 0.1～6g, the metal ion of 0.001～0.04mol and the distilled water of surplus; Wherein the carbon source nutritive ingredient is one or more in glucose, sucrose, the starch syrup; The nitrogenous source nutritive ingredient is one or more in corn steep liquor, peptone, the yeast extract paste; Metal ion is one or more in mn ion, magnesium ion, the zine ion.
2. the fermention medium that short antioxidant according to claim 1 generates is characterized in that the every L of fermention medium that short antioxidant generates is made up of the carbon source nutritive ingredient of 10～90g, the nitrogenous source nutritive ingredient of 0.5～5g, the metal ion of 0.002～0.03mol and the distilled water of surplus.
3. the fermention medium that short antioxidant according to claim 1 generates is characterized in that the every L of fermention medium that short antioxidant generates is made up of the carbon source nutritive ingredient of 30g, the nitrogenous source nutritive ingredient of 2g, the metal ion of 0.005mol and the distilled water of surplus.
4. the fermention medium that short antioxidant according to claim 1 generates is characterized in that the every L of fermention medium that short antioxidant generates is made up of the carbon source nutritive ingredient of 50g, the nitrogenous source nutritive ingredient of 3g, the metal ion of 0.01mol and the distilled water of surplus.
5. the fermention medium that short antioxidant according to claim 1 generates is characterized in that the every L of fermention medium that short antioxidant generates is made up of the carbon source nutritive ingredient of 60g, the nitrogenous source nutritive ingredient of 4g, the metal ion of 0.02mol and the distilled water of surplus.
6. the fermention medium that generates according to claim 1,2,3,4 or 5 described short antioxidants is characterized in that mn ion derives from MnSO
4H
2O.
7. the fermention medium that short antioxidant according to claim 6 generates is characterized in that magnesium ion derives from MgSO
47H
2O.
8. the fermention medium that short antioxidant according to claim 7 generates is characterized in that zine ion derives from ZnCl
2
9. the fermention medium that short antioxidant generates is characterized in that the every L of fermention medium that short antioxidant generates is made up of the carbon source nutritive ingredient of 5～100g, the nitrogenous source nutritive ingredient of 0.1～6g, the agar of 1～100g, the metal ion of 0.001～0.04mol and the distilled water of surplus; Wherein the carbon source nutritive ingredient is one or more in glucose, sucrose, the starch syrup; The nitrogenous source nutritive ingredient is one or more in corn steep liquor, peptone, the yeast extract paste; Metal ion is one or more in mn ion, magnesium ion, the zine ion.
10. the fermention medium that short antioxidant according to claim 9 generates is characterized in that the every L of fermention medium that short antioxidant generates is made up of the carbon source nutritive ingredient of 15g, the nitrogenous source nutritive ingredient of 1g, the agar of 10g, the metal ion of 0.03mol and the distilled water of surplus.
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| CN201110127680XA CN102250955A (en) | 2011-05-17 | 2011-05-17 | Fermentation medium for promoting generation of antioxidant |
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|---|---|---|---|
| CN201110127680XA CN102250955A (en) | 2011-05-17 | 2011-05-17 | Fermentation medium for promoting generation of antioxidant |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1456666A (en) * | 2003-03-20 | 2003-11-19 | 中国科学院生态环境研究中心 | Production of pectase by solid mould oxalate fermenting |
| CN1807607A (en) * | 2006-01-20 | 2006-07-26 | 安徽大学 | Method for fermentative production of laccase using fungoid co-cultivated |
| CN1844407A (en) * | 2005-04-06 | 2006-10-11 | 北京化工大学 | Method for simultaneous production of ergosterol and glutathione by yeast fermentation |
| CN101220335A (en) * | 2007-12-28 | 2008-07-16 | 中国烟草总公司郑州烟草研究院 | Bacterial strain for preventing and controlling tobacco black shank and bacterium agent thereof |
| CN101760489A (en) * | 2008-12-23 | 2010-06-30 | 上海医药工业研究院 | Fermentation medium for producing Mizoribine |
-
2011
- 2011-05-17 CN CN201110127680XA patent/CN102250955A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1456666A (en) * | 2003-03-20 | 2003-11-19 | 中国科学院生态环境研究中心 | Production of pectase by solid mould oxalate fermenting |
| CN1844407A (en) * | 2005-04-06 | 2006-10-11 | 北京化工大学 | Method for simultaneous production of ergosterol and glutathione by yeast fermentation |
| CN1807607A (en) * | 2006-01-20 | 2006-07-26 | 安徽大学 | Method for fermentative production of laccase using fungoid co-cultivated |
| CN101220335A (en) * | 2007-12-28 | 2008-07-16 | 中国烟草总公司郑州烟草研究院 | Bacterial strain for preventing and controlling tobacco black shank and bacterium agent thereof |
| CN101760489A (en) * | 2008-12-23 | 2010-06-30 | 上海医药工业研究院 | Fermentation medium for producing Mizoribine |
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Application publication date: 20111123 |