CN102250809B - Wheat rhizosphere azotobacter for antagonism of various pathogenic fungi and application of wheat rhizosphere azotobacter - Google Patents
Wheat rhizosphere azotobacter for antagonism of various pathogenic fungi and application of wheat rhizosphere azotobacter Download PDFInfo
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Abstract
The invention discloses a wheat rhizosphere azotobacter for antagonism of various pathogenic fungi and application of the wheat rhizosphere azotobacter. The wheat rhizosphere azotobacter is Paenibacillus sp. GD641, and has the collection number of CGMCC No.5042 in China General Microbiological Culture Collection Center. The Paenibacillus sp. GD641 with the collection number of CGMCC No.5042 has effective antagonism on Gibberella zeae, Sclerotinia sclerotiorum and Verticillium dahlia of crops, and has wide application prospect in microbial azotobacter and biological organic fertilizer production.
Description
Technical field
The present invention relates to wheat rhizosphere vinelandii of antagonism Various Diseases fungal pathogens and uses thereof, particularly a kind of can antagonism crop gibberellic hypha, sclerotium germ and verticillium wilt pathogen, and have the bacterium that carries out the high-efficiency nitrogen-fixing function at wheat rhizosphere.
Background technology
Nitrogenous fertilizer is one of most important production means in the agriculture production.Produce chemical nitrogen fertilizer and consume mass energy, account for 70%～80% of total production cost.Energy day is becoming tight in recent years, and China's chemical fertilizers production soars with the coal price lattice and causes the chemical nitrogen fertilizer price increase, and the peasant is difficult to bear, and repercussion is strong.In addition, chemical nitrogen fertilizer is being brought into play great function aspect raising crop yield, the guarantee China grain security, but the unreasonable nitrogen of executing has formed serious pollution of area source, causes a series of serious harm, makes us startling such as the Taihu Lake Blue Algae Event.The urban groundwater azotate pollution has caused threat to drinking water safety.
Contain 78% nitrogen in the atmosphere, but its existence form is molecular state nitrogen, animals and plants can not directly utilize, and nature only has some prokaryotic micro-organisms to have the ability of directly utilizing nitrogen in the atmosphere, with its reduction ammonification, biological nitrogen fixation that Here it is.Select the batch production of high-efficiency nitrogen-fixing microorganism bacterial classification to produce and make microbial inoculum, or further become biological products with other material Compound Machining that is rich in plant nutrition, be applied to agriculture production, crop nitrogen nutrition can be provided, improve the crop rhizosphere ecotope, improve the soil biological fertility proterties, Here it is usually said nitrogen-fixing microorganism microbial inoculum or nitrogen-fixing microorganism fertilizer.Biological nitrogen fertilizer has multiple advantage: 1. biological nitrogen fertilizer is comprised of reproducible biochemical preparation and living microorganism, can regenerate, and does not have the resource exhaustion problem, is the Sustainable development agricultural material product.2. biological nitrogen fertilizer is environmental friendly fertilizer, and it is produced and use procedure does not all produce the pollutants such as the three wastes, and can improve soil physico-chemical property, increase soil fertility, and be ecological agriculture agricultural material product.3. biological nitrogen fertilizer production power consumption less, cost is low, and its cost only is 20%～40% of equivalent chemical nitrogen fertilizer, can save a large amount of coal and oil equal energy source strategic materials for country.4. use biological nitrogen fertilizer can significantly reduce agriculture production cost, improve quality of agricultural product, promote soil fertility, the peasant is benefited, development promotes social harmony.
The pathogenic bacteria of crop head blight is that Gibberella zeae bacterium (Gibberella zeae) asexual generation is Fusarium graminearum (Fusarium graminearum), is the important pathogenic bacteria of food crop.China's wheat scab has 95% to be that Gibberella zeae (Sch.) Petch is microbial, is the important disease of middle and lower reach of Yangtze River Winter Wheat Area and Northeasten Spring Wheat Area of China, often causes 20%～30% production loss during morbidity.In addition, this bacterium is also infected the crops such as corn, paddy rice, barley, causes that seedling is withered, stem rot, base is rotten, fringe is rotten etc.The pathogenic bacteria of sclerotium disease is sclerotinite (Sclerotinia sclerotiorum), its morphological specificity shows as: apothecium is little, be little cup-shaped, light salmon is to brown, single or severally bears from sclerotium, diameter 0.5-1cm, the handle brown is elongated, bending, long 3-5cm, gradually thin downwards, link to each other with sclerotium.Mycelium can form sclerotium, and the brown apothecium of long handle is created on the sclerotium.The sclerotium shape is various, long 3-15 μ m.Ascus is cylindrical, 120-140 μ m * 11 μ m, and common 8 of spore, single file is arranged, ellipse, 8-14 μ m * 4-8 μ m, lateral filament is elongated, and is linear, colourless, and the top is thicker.This bacterium is the global important phytopathogen of a kind of damage to crops and vegetables, infect widely, endanger the plants such as Cruciferae, pulse family, Solanaceae, Rutaceae, as cause cash crop and the vegetables sclerotium diseases such as rape, soybean, Sunflower Receptacle, cucumber, capsicum.Verticillium dahliae (verticillium dahliae Verticillium dahliae) is take the soil propagation as main, host range is very wide, cause the verticillium except infecting cotton, also infect cash crop tobacco, oil crops soybean, peanut, Sunflower Receptacle, sesame, vegetable crop potato, eggplant, capsicum, tomato, cucumber, watermelon, and forest, flowers etc., harm is serious, causes the tremendous economic loss.At present the control of above-mentioned fungal disease mainly relied on chemical pesticide, yet not only cost is high, contaminate environment in chemical prevention, and preventive effect is also undesirable, the simultaneously security of food is also had a strong impact on.
Bacterial classification is the basis of microbial fertilizer production application.At present, the bottleneck of restriction China microbial fertilizer industry development is exactly the seed selection problem of high-efficiency strain.Agriculture production active demand nitrogen fixation efficiency height, antagonizing pathogenic fungi, strong stress resistance, the long microbial fertilizer production bacterial classification of shelf-lives.
Summary of the invention
The purpose of this invention is to provide a strain can carry out high-efficiency nitrogen-fixing at crop rhizosphere, and the bacterium of antagonism crop gibberellic hypha (Gibberella zeae), sclerotium germ (Sclerotinia sclerotiorum) and verticillium dahliae (Verticillium dahliae).
Rhizosphere azotobacter provided by the present invention, series bacillus (Paenibacillus sp.) GD641, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on July 6th, 2011 and (is called for short CGMCC, the address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City), deposit number is CGMCC No.5042.
Another object of the present invention provides a kind of microbial inoculum, and the activeconstituents of this microbial inoculum is described series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042.
This microbial inoculum also can comprise auxiliary material, such as the stalk of the ight soil of the peat composed of rotten mosses, animal, all kinds of crops, loose shell, straw, peanut skin etc. except series bacillus (Paenibacillus sp.) the GD641 CGMCC No.5042 that comprises as activeconstituents.
This microbial inoculum can be used for suppressing pathogenic fungi, control fungal diseases of plants, fixed nitrogen, generation nitrogenase etc.
Described series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042 is in preparation following 1)-4) in application in arbitrary microbial inoculum also belong to protection scope of the present invention:
1) for the microbial inoculum that suppresses pathogenic fungi;
2) for the microbial inoculum of preventing and treating fungal diseases of plants;
3) be used for the microbial inoculum of fixed nitrogen;
4) microbial inoculum of generation nitrogenase.
Described pathogenic fungi can be by soil, the fertilizer in being manured into soil and/or the fungi of seed dispersal, specifically can be the fungi or fungi or the fungi for causing verticillium for causing sclerotium disease that cause head blight; Described fungal diseases of plants can be head blight, sclerotium disease or verticillium.
Describedly cause that the fungi of head blight can be Gibberella zeae bacterium (Gibberella zeae) or Fusarium graminearum (Fusarium grammearum); Describedly cause that the fungi of sclerotium disease can be sclerotinite (Sclerotinia sclerotiorum); Describedly cause that the fungi of verticillium can be verticillium dahliae (Verticillium dahliae).
Application and the application in the preparation biological organic fertilizer in producing nitrogenase of described series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042 or the microbial inoculum take this series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042 as activeconstituents also belongs to protection scope of the present invention.
A further object of the present invention provides a kind of described series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042 or biological organic fertilizer take described series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042 as the microbial inoculum of activeconstituents of containing.
Another purpose of the present invention provides the method for a kind of cultivation series bacillus (Paenibacillus sp.) GD641 CGMCCNo.5042, and the method comprises the step that series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042 is cultivated at the substratum that is used for the cultivation series bacillus.
Another purpose of the present invention provides a kind of method for preparing described microbial inoculum, and the method comprises the steps: described series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042 to obtain described microbial inoculum as activeconstituents.
Experimental results show that, the present invention is through layer by layer screening from pedotheque, finishing screen has been selected series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042, this bacterial strain has very high nitrogenase activity, can antagonism crop head blight pathogenic bacteria Gibberella zeae bacterium (Gibberella zeae), sclerotium disease pathogenic bacteria sclerotinite (Sclerotinia sclerotiorum) and verticillium dahliae (verticillium dahliae Verticillium dahliae), compete adaptable, effect of inoculation is good, has broad application prospects in nitrogen-fixing microorganism microbial inoculum and biological organic fertilizer production.
The preservation explanation
Strain name: series bacillus
Latin name: (Paenibacillus sp.)
Strain number: GD641
Preservation mechanism: China Committee for Culture Collection of Microorganisms common micro-organisms center
Preservation mechanism is called for short: CGMCC
Address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City
Preservation date: on July 6th, 2011
The preservation center numbering of registering on the books: CGMCC No.5042
Description of drawings
Fig. 1 is the nitrogenase activity of series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042.
Fig. 2 is series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042 antagonism gibberellic hypha Gibberella zeae bacterium (Gibberella zeae).The left side bacterium colony is series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042, and the right side bacterium colony is gibberellic hypha Gibberella zeae bacterium (Gibberella zeae).
Fig. 3 is series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042 antagonism sclerotium germ (Sclerotinia sclerotiorum).The left side bacterium colony is series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042, and the right side bacterium colony is sclerotium germ (Sclerotinia sclerotiorum).
Fig. 4 is series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042 antagonism verticillium dahliae (Verticillium dahliae).The left side bacterium colony is series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042, and the right side bacterium colony is verticillium dahliae (Verticillium dahliae).
Embodiment
Employed experimental technique is ordinary method if no special instructions among the following embodiment.
Used material, reagent etc. if no special instructions, all can obtain from commercial channels among the following embodiment.
Vinelandii enrichment culture liquid ACCC55 composition: sucrose 10g, K
2HPO
43H
2O 0.5g, NaCl 0.2g, CaCO
31g, MgSO
47H
2O 0.2g, distilled water 1000ml, pH 7.0～7.2.
The ACCC55 vinelandii solid medium composition of improvement: sucrose 10g, K
2HPO
43H
2O 0.5g, NaCl 0.2g, CaCO
31g, MgSO
47H
2O 0.2g, yeast extract paste 0.5g, distilled water 1000ml, agar 1.5%～2.0%, pH 7.0～7.2.
Nitrogen-free agar: sucrose 10g, NaCl 0.12g, K
2HPO
43H
2O 0.5g, CaCO
31g, MgSO
47H
2O 0.2g, distilled water 1000mL, pH7.2.
Azotobacter chroococcum (Azotobacter chroococcum) ACCC11103 (referring to " Sun Jianguang etc. the screening of high-efficiency nitrogen-fixing genus bacillus and biological characteristics thereof. Scientia Agricultura Sinica, 2009,42 (6): 2043-2051 ").
Gibberellic hypha-Gibberella zeae bacterium (Gibberella zeae) (Chinese agriculture microbial strains preservation administrative center, ACCC31053).
Sclerotium germ-sclerotinite (Sclerotinia sclerotiorum) (Chinese agriculture microbial strains preservation administrative center, ACCC30046).
Verticillium dahliae-Garden Dahlia wheel branch spore (Verticillium dahliae) (Chinese agriculture microbial strains preservation administrative center, ACCC30308).
Separation and the evaluation of embodiment 1, wheat rhizosphere vinelandii GD641
One, the enrichment of wheat rhizosphere vinelandii GD641 with separate
Getting 10g soil sample (picking up from the inner mongolia) puts into 90ml sterilized water shaking table vibration 20min and makes dirty solution, draw 5ml and put into 30ml vinelandii enrichment culture liquid ACCC55,100rpm, 28 ℃ are carried out the shaking table shaking culture, renew bright nutrient solution behind the 72h and continue to cultivate.Carrying out the fixed nitrogen sporeformer after repeating to cultivate 3 times separates.The above-mentioned vinelandii enrichment culture of absorption 1ml thing is put in the 9ml sterilized water makes 10
-1Extent of dilution continues dilution and makes 10
-2, 10
-3, 10
-4, 10
-5Dilution bacteria suspension heats 10min in 100 ℃ of boiling water, rear each extent of dilution of cooling is got 0.1ml and is coated on the vinelandii isolation medium flat board, and 29 ℃ leave standstill cultivation.2～3d carries out purifying agaric with plate streak on the ACCC55 vinelandii culture medium flat plate of improvement after bacterium colony forms.One of them bacterial strain called after wheat rhizosphere vinelandii GD641 with the separation and purification gained.
Two, the evaluation of wheat rhizosphere vinelandii GD641
From the wheat rhizosphere vinelandii that the following aspects authentication step one is separated and purifying obtains:
1, Morphological Identification
To be in logarithmic phase, and the bacterium colony size is stable, the vinelandii GD641 that above-mentioned steps one is separated and purifying obtains carries out single bacterium colony state description, mainly comprises size, color, transparency, wettability, bacterium colony condition of surface (whether smooth, projection, fold, depression etc.), the colony edge state (whether neat, irregular, radial etc.) of bacterium colony.
For the described vinelandii GD641 that is in logarithmic phase, behind smear staining, adopt the form of observation by light microscope thalline.
The result shows, the vinelandii GD641 bacterium colony circular protrusions that above-mentioned steps one is separated and purifying obtains, and drops, transparent, glossy, surface wettability is smooth, the edge haloing; Thalline is shaft-like, and 0.5～1.0 * 0.5～1.0 μ m are without gemma.
2, analysis of physio biochemical characteristics
With reference to " common bacteria system identification handbook (eastern elegant pearl, Cai Miaoying. common bacteria system identification handbook. Beijing: Science Press, 2011.) and " Microbiology Experiment " (Shen Ping, Fan Xiurong, Li Guangwu. Microbiology Experiment (third edition). Beijing: Higher Education Publishing House, 1999.) measure the physiological and biochemical property of above-mentioned vinelandii GD641.
The physiological and biochemical property measurement result of described vinelandii GD641 is as follows:
Catalase reaction: the positive
Oxydase reaction: feminine gender
Growth temperature: do not grow for 4 ℃, 28 ℃ of growths, 37 ℃ of growths or not for 60 ℃
The salt tolerance test: the 2%NaCl growth, 5%NaCl does not grow, and 7%NaCl does not grow, and 10%NaCl does not grow
Phenylalanine deaminase test: feminine gender
Utilize Citrate trianion: feminine gender
Hydrolyzed starch: the positive
Yolk lecithin enzyme: the positive
Methyl red test: feminine gender
V.P test: feminine gender
PH 5.7 growths: the positive
The sugar alcohol fermentation and acid: glucose is positive, and N.F,USP MANNITOL is positive, and lactose is positive, and sucrose is positive
3,16s rDNA sequence homology analysis
Ordinary method is cultivated the vinelandii GD641 that above-mentioned steps one separation and purification obtains, extract total DNA of bacterial strain as the gene amplification template, with bacterium 16s rDNA universal primer, 27f:5 '-AGAGTTTGATCCTGGCTCAG-3 ', 1492r:5 '-TACGGTTACCTTGTTACGACTT-3 ' carry out the PCR reaction.Reaction system adopts Shanghai biotechnology company limited pcr amplification test kit.Response procedures is: 95 ℃ of sex change 30s, 55 ℃ of annealing 1min, 72 ℃ of extensions 2min, totally 30 circulations.Dna sequencing is finished by Beijing three rich polygala root biotech companies, sequence assembly and similarity analysis use DNAStar software to finish, and sequence alignment is finished online by American National biotechnology information center ncbi database (http://www.ncbi.nlm.nih.gov).
The sequence of vinelandii GD641 bacterial strain 16s rDNA sees sequence 1 in the sequence table for details.
4, growth characteristics analysis
Bacterial strain optimum temperuture and optimal pH growth experiment have been carried out.Adopt nitrogen-free agar, at the thermal adaptability of 4 ℃, 28 ℃, 37 ℃, 60 ℃ cultivations, observation, record bacterial strain, each is processed 3 times and repeats respectively.Adjust acidity and be respectively pH3, pH4, pH5, pH6, pH7, pH8, pH9, pH10, pH11, each is processed 3 times and repeats, the optimal pH of cultivation, observation, record strain growth.
The result shows that the optimum growth temperature of described wheat rhizosphere vinelandii GD641 is 28 ℃, and the most suitable growth pH is pH7～8.
In view of above-mentioned form, analysis of physio biochemical characteristics and 16s rDNA sequence homology analysis result, the wheat rhizosphere vinelandii GD641 that step 1 is separated and purifying obtains is accredited as modification bacterial alpha subgroup series bacillus section series bacillus belongs to (Paenibacillus sp.).This wheat rhizosphere vinelandii GD641 is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on July 6th, 2011 and (is called for short CGMCC, the address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City), deposit number is CGMCC No.5042.
Embodiment 2, series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042 nitrogenase activity determination
Embodiment 1 resulting series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042 is carried out nitrogenase activity determination, concrete grammar is as described below: add 5ml improvement fixed nitrogen substratum bevel in 15 * 150mm screw socket Glass tubing, Azotobacter, 28 ℃ of cultivations.With the positive contrast of microbial fertilizer production commonly used bacterial classification azotobacter chroococcum (Azotobacter chroococcum) ACCC11103, do not inoculate the negative contrast in blank inclined-plane, establish 3 repetitions.After cultivating 72h, change rubber plug, it is 10% that the injection acetylene gas makes final concentration, seal with medical proof fabric, continue to cultivate 72h, get 100 μ l reactant gasess, with gas chromatograph for determination ethene growing amount, according to the nitrogenase activity of following formula compute classes genus bacillus (Paenibacillus sp.) GD641 CGMCC No.5042.Nitrogenase activity (nmol/mgh)=C
2H
4Nmol/[tropina amount (mg) * reaction times (h)], C wherein
2H
4Nmol=1000 * C
2H
4Volume (μ l) * 273 * P/[22.4 * (273+t) * 760], wherein P is air pressure (mm mercury column), t be temperature of reaction (℃).
Wherein, the tropina content assaying method is as described below: with 5ml physiological saline the lawn on the test tube slant is washed in the centrifuge tube, collect thalline, the NaOH boiling water that adds 3ml 0.5M in the precipitation boils 5min, and the HCl that adds 3ml 0.5M mixes, and gets supernatant 1.0ml after centrifugal, add 5ml Xylene Brilliant Cyanine G solution, mix colour developing 3min, the light absorption value A at mensuration 595nm place at eddy mixer
595, calculate tropina content according to the bovine serum albumin typical curve.
The result shows that the nitrogenase activity of the series bacillus that screens (Paenibacillus sp.) GD641 CGMCC No.5042 is 27.357nmol C
2H
4/ hmg albumen, statistical study and the microbial fertilizer nitrogenase activity 25.100nmol C that produces bacterial classification azotobacter chroococcum ACCC11103 commonly used
2H
4/ hmg albumen indifference, as shown in Figure 1.This result shows that series bacillus of the present invention (Paenibacillus sp.) GD641 CGMCC No.5042 can high-efficiency nitrogen-fixing.
Embodiment 3, series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042 antagonizing pathogenic fungi bacteriostasis rate are measured
Adopting 2 face-off methods that embodiment 1 resulting series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042 is carried out the antagonizing pathogenic fungi bacteriostasis rate measures, concrete operations are as described below: inoculate respectively crop gibberellic hypha (Gibberella zeae) (perhaps sclerotium germ (Sclerotinia sclerotiorum) on 2 of distance center 2cm on the PDA flat board, perhaps verticillium dahliae (Verticillium dahliae)) and series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042,3 repetitions of each Screening Treatment do not connect the flat board of vinelandii as contrast only to connect pathogenic fungi.28 ℃ of constant temperature culture are measured the dull and stereotyped upper pathogenic fungi of face-off along the colony radius r of tested series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042 direction with the millimeter graduated scale behind the 15d
1, and the colony radius r of the dull and stereotyped upper pathogenic fungi of contrast
0Pathogenic fungi growth inhibition ratio (%)=(contrast radius r
0Pathogenic fungi colony radius r is cultivated in-face-off
1)/contrast radius r
0* 100%.
The result shows, the r of gibberellic hypha (Gibberella zeae)
063.3 ± 2.8mm, r
126.6 ± 0.8mm; The r of sclerotium germ (Sclerotinia sclerotiorum)
063.0 ± 2.1mm, r
127.3 ± 1.5mm; The r of verticillium dahliae (Verticillium dahliae)
041.0 ± 1.9mm, r
121.7 ± 1.1mm.Mean value is calculated for the above-mentioned formula of people: described series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042 is 57.98% to the bacteriostasis rate of gibberellic hypha (Gibberella zeae), as shown in Figure 2; Bacteriostasis rate to sclerotium germ (Sclerotinia sclerotiorum) is 56.67%, as shown in Figure 3; Bacteriostasis rate to verticillium dahliae (Verticillium dahliae) is 47.07%, as shown in Figure 4.This result shows described series bacillus (Paenibacillus sp.) GD641 CGMCC No.5042 effectively antagonism crop gibberellic hypha (Gibberella zeae), sclerotium germ (Sclerotinia sclerotiorum) and verticillium dahliae (Verticillium dahliae).
Claims (10)
1. series bacillus (Paenibacillus sp.) GD641, its deposit number at China Committee for Culture Collection of Microorganisms common micro-organisms center is CGMCC No.5042.
2. microbial inoculum, its activeconstituents is series bacillus claimed in claim 1 (Paenibacillus sp.) GD641CGMCC No.5042.
3. series bacillus claimed in claim 1 (Paenibacillus sp.) GD641CGMCC No.5042 is in preparation following 1)-4) in application in the arbitrary microbial inoculum:
1) for the microbial inoculum that suppresses pathogenic fungi;
2) for the microbial inoculum of preventing and treating fungal diseases of plants;
3) be used for the microbial inoculum of fixed nitrogen;
4) microbial inoculum of generation nitrogenase.
4. application according to claim 3 is characterized in that: described pathogenic fungi is fungi or fungi or the fungi for causing verticillium for causing sclerotium disease that causes head blight; Described fungal diseases of plants is head blight, sclerotium disease or verticillium.
5. application according to claim 4 is characterized in that: describedly cause that the fungi of head blight is Gibberella zeae bacterium (Gibberella zeae) or Fusarium graminearum (Fusarium graminearum); Describedly cause that the fungi of sclerotium disease is sclerotinite (Sclerotinia sclerotiorum); Describedly cause that the fungi of verticillium is verticillium dahliae (Verticillium dahliae).
6. series bacillus claimed in claim 1 (Paenibacillus sp.) GD641CGMCC No.5042 or the described microbial inoculum of claim 2 are used in producing nitrogenase.
7. series bacillus claimed in claim 1 (Paenibacillus sp.) GD641CGMCC No.5042 or the described microbial inoculum of claim 2 are used in the preparation biological organic fertilizer.
8. the biological organic fertilizer that contains the described series bacillus of claim 1 (Paenibacillus sp.) GD641CGMCC No.5042 or the described microbial inoculum of claim 2.
9. cultivate the method for the described series bacillus of claim 1 (Paenibacillus sp.) GD641CGMCC No.5042, comprise the step that series bacillus (Paenibacillus sp.) GD641CGMCC No.5042 is cultivated at the substratum that is used for the cultivation series bacillus.
10. the preparation method of the described microbial inoculum of claim 2 comprises the steps: series bacillus claimed in claim 1 (Paenibacillus sp.) GD641CGMCC No.5042 is obtained described microbial inoculum as activeconstituents.
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| CN108728380A (en) * | 2018-06-06 | 2018-11-02 | 浙江省农业科学院 | One plant to bacterial strain of the Chinese cabbage hyphal cluster germ with antagonism and its cultural method and application |
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| KR100995311B1 (en) * | 2008-04-22 | 2010-11-19 | 재단법인서울대학교산학협력재단 | Paenibacillus polymyxa GBR508 strain having antifungal, nematicidal activity and plant growth-promoting effect and method for controlling plant disease complex and promoting plant growth using the same |
| CN101696395B (en) * | 2009-10-29 | 2010-10-27 | 南京农业大学 | Antagonistic bacterium for preventing and killing off continuous cropping tobacco black shank and microbial organic fertilizer thereof |
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| CN108728380B (en) * | 2018-06-06 | 2020-07-31 | 浙江省农业科学院 | Strain with antagonistic action on Chinese cabbage sclerotinia sclerotiorum and culture method and application thereof |
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