CN102250167A - Process for separating rebaudioside from stevioside - Google Patents
Process for separating rebaudioside from stevioside Download PDFInfo
- Publication number
- CN102250167A CN102250167A CN2011101410033A CN201110141003A CN102250167A CN 102250167 A CN102250167 A CN 102250167A CN 2011101410033 A CN2011101410033 A CN 2011101410033A CN 201110141003 A CN201110141003 A CN 201110141003A CN 102250167 A CN102250167 A CN 102250167A
- Authority
- CN
- China
- Prior art keywords
- stevioside
- rebaudioside
- sugar
- methyl alcohol
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000019202 steviosides Nutrition 0.000 title claims abstract description 15
- UEDUENGHJMELGK-HYDKPPNVSA-N Stevioside Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O UEDUENGHJMELGK-HYDKPPNVSA-N 0.000 title claims abstract description 13
- 229940013618 stevioside Drugs 0.000 title claims abstract description 13
- OHHNJQXIOPOJSC-UHFFFAOYSA-N stevioside Natural products CC1(CCCC2(C)C3(C)CCC4(CC3(CCC12C)CC4=C)OC5OC(CO)C(O)C(O)C5OC6OC(CO)C(O)C(O)C6O)C(=O)OC7OC(CO)C(O)C(O)C7O OHHNJQXIOPOJSC-UHFFFAOYSA-N 0.000 title claims abstract description 13
- 229930188195 rebaudioside Natural products 0.000 title claims abstract description 10
- 238000000034 method Methods 0.000 title abstract description 5
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 39
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 17
- 238000002425 crystallisation Methods 0.000 claims description 6
- 230000008025 crystallization Effects 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 238000001953 recrystallisation Methods 0.000 claims description 3
- 239000013078 crystal Substances 0.000 claims description 2
- 238000000746 purification Methods 0.000 claims description 2
- 235000019658 bitter taste Nutrition 0.000 abstract description 3
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 3
- 235000013373 food additive Nutrition 0.000 abstract 1
- 239000002778 food additive Substances 0.000 abstract 1
- 235000003599 food sweetener Nutrition 0.000 abstract 1
- 239000003765 sweetening agent Substances 0.000 abstract 1
- 235000019640 taste Nutrition 0.000 abstract 1
- 235000019441 ethanol Nutrition 0.000 description 4
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000012043 crude product Substances 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
Landscapes
- Seasonings (AREA)
Abstract
The invention relates to an improved method for separating rebaudioside from sweetener stevioside contained in a food additive. The method comprises the following steps of: separating RA sugar from common RA-50 stevioside through methanol; then recrystallizing by using ethanol to obtain RA-90 sugar; then purifying by using the methanol to obtain the rebaudioside. According to the method disclosed by the invention, the produced rebaudioside of the stevioside reaches more than 99.5 percent and has good taste without lingering bitterness and heavy metal lower than 100 PPB.
Description
Technical field
The present invention is a kind of improvement that the sweeting agent stevioside separates the rebaudioside method in the foodstuff additive.
Background technology
At present, be well known that crude product sugar separated STV sugar with methyl alcohol after, use ethyl alcohol recrystallization again, obtain RA-95% sugar.Purity is not high enough, and assorted point is bigger, and mouthfeel is impure, and back bitter taste is dense, the heavy metal height.
Summary of the invention
The present invention is directed to above-mentioned defective, purpose is to provide that a kind of purity is higher, the better stevioside of mouthfeel separates rebaudioside technology.
Technical scheme of the present invention is: the present invention separates RA sugar with common RA-50 Steviosides by 6 times of methyl alcohol, and the back makes with methanol purification with obtaining RA-90 sugar behind 5 times of recrystallizations of 85% ethanol again.
It is 6 times of 90% methyl alcohol weight parts that the present invention adds earlier concentration in the stevioside, and at room temperature being stirred to crystal, to separate out the back centrifugal, dry; Be 5 times of dissolvings of ethanol weight part of 85%, leave standstill crystallization in 6 hours, centrifugal drying with concentration again; Be 6 times of dissolvings of methyl alcohol weight part of 80% again with concentration, leave standstill crystallization in 6 hours after centrifugal drying make.
Advantage of the present invention is:: its rebaudioside of stevioside of producing according to the present invention reaches more than 99.5%; Mouthfeel is good, not the back bitter taste; Heavy metal is below 100PPB.
Embodiment
Is 1: 6 times of stirred crystallization with common RA-50 stevioside by 90% methyl alcohol weight ratio, leaves standstill after 6 hours centrifugal, dry; Use 5 times of dissolvings of 85% ethanol weight part again, crystallisation by cooling obtains RA-90 sugar behind the centrifugal drying, is 80% the mellow centrifugal RA-99.5% that makes of dissolve with methanol xln then with concentration.
Claims (2)
1. stevioside separates rebaudioside technology, and common RA-50 Steviosides is separated RA sugar by 6 times of methyl alcohol, and the back is characterized in that with obtaining RA-90 sugar behind 5 times of recrystallizations of 85% ethanol, makes with methanol purification.
2. stevioside according to claim 1 separates rebaudioside technology, it is characterized in that, adds concentration earlier in the stevioside and be 6 times of 90% methyl alcohol weight parts, and at room temperature being stirred to crystal, to separate out the back centrifugal, dry; Be 5 times of dissolvings of ethanol weight part of 85%, leave standstill crystallization in 6 hours, centrifugal drying with concentration again; Be 6 times of dissolvings of methyl alcohol weight part of 80% again with concentration, leave standstill crystallization in 6 hours after centrifugal drying make.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101410033A CN102250167A (en) | 2011-05-30 | 2011-05-30 | Process for separating rebaudioside from stevioside |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101410033A CN102250167A (en) | 2011-05-30 | 2011-05-30 | Process for separating rebaudioside from stevioside |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102250167A true CN102250167A (en) | 2011-11-23 |
Family
ID=44977771
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011101410033A Pending CN102250167A (en) | 2011-05-30 | 2011-05-30 | Process for separating rebaudioside from stevioside |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102250167A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105061527A (en) * | 2015-08-31 | 2015-11-18 | 湖南威嘉生物科技有限公司 | Process for refined production of stevioside |
| CN107820394A (en) * | 2015-07-10 | 2018-03-20 | 帝斯曼知识产权资产管理有限公司 | Steviol glycoside composition |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09107913A (en) * | 1995-10-19 | 1997-04-28 | Morita Kagaku Kogyo Kk | Alpha-glucosylated stevia sweetener |
| CN101250204A (en) * | 2008-03-27 | 2008-08-27 | 青岛润德生物科技有限公司 | Method for refining steviosides STV |
| CN101830947A (en) * | 2010-05-05 | 2010-09-15 | 宋英海 | Method for making high-content rebaudioside-A stevioside |
-
2011
- 2011-05-30 CN CN2011101410033A patent/CN102250167A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09107913A (en) * | 1995-10-19 | 1997-04-28 | Morita Kagaku Kogyo Kk | Alpha-glucosylated stevia sweetener |
| CN101250204A (en) * | 2008-03-27 | 2008-08-27 | 青岛润德生物科技有限公司 | Method for refining steviosides STV |
| CN101830947A (en) * | 2010-05-05 | 2010-09-15 | 宋英海 | Method for making high-content rebaudioside-A stevioside |
Non-Patent Citations (2)
| Title |
|---|
| 张杨，等: "重结晶法分离精制莱鲍迪试A的研究", 《离子交换与吸附》, vol. 14, no. 6, 20 December 1998 (1998-12-20), pages 515 - 520 * |
| 胡滨，等: "甜菊糖中A3甙分离方法初探", 《湖北三峡学院学报》, vol. 19, no. 3, 31 August 1997 (1997-08-31), pages 94 - 96 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107820394A (en) * | 2015-07-10 | 2018-03-20 | 帝斯曼知识产权资产管理有限公司 | Steviol glycoside composition |
| US11066688B2 (en) | 2015-07-10 | 2021-07-20 | Dsm Ip Assets B.V. | Method for preparing a steviol glycoside composition |
| CN105061527A (en) * | 2015-08-31 | 2015-11-18 | 湖南威嘉生物科技有限公司 | Process for refined production of stevioside |
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Application publication date: 20111123 |