CN102241785A - Production technology of purslane polysaccharide - Google Patents

Abstract

The invention relates to a production technology of purslane polysaccharide. The production technology comprises the following steps that: impurities in purslane are removed; purslane is washed, cut into segments, and dried; processed purslane is immersed in water for 30 minutes, and is extracted 3 times through microwave auxiliary extraction; in a vacuum heating condition, pressure reduction concentration is carried out on the purslane extract, such that a condensed purslane extract is obtained; a small amount of ethanol is added to the condensed purslane extract, and the mixture is stirred and well-mixed; the mixture is then stood for 24 hours, and a precipitate is obtained; the precipitate is diluted with a proper amount of water; chloroform and n-butanol are added to the diluted precipitate, and the mixture is mixed and oscillated for 30 minutes. Obtained protein-removed crude purslane polysaccharide is diluted with a small amount of water, and the diluted crude purslane polysaccharide is eluted in macroporous adsorption resin; active carbon is added to a polyamide eluate for adsorbing, and active carbon is removed; hydrogen peroxide is added to the obtained material, the mixture is well-mixed, decolored, and heated; residual hydrogen peroxide is then removed; the obtained material is vacuum-dried, such that purslane polysaccharide with a relatively high purity is obtained. The produced purslane polysaccharide has functions of immunity improving, tumor resisting, oxidation resisting, and aging resisting. The purslane polysaccharide has a hypoglycemic effect, and can be used for changing diabetes symptoms. Purslane polysaccharide has a good anti-tumor function, and can be applied in the development of anti-tumor medicines.

Description

The Polysaccharide from Portulaca oleracea production technique

Technical field:

The present invention relates to purslane, particularly a kind of Polysaccharide from Portulaca oleracea production technique.

Background technology:

Purslane is a Portulacaceae Portulaca annual herb plant.Be commonly called as longevity greens/mustard green, the horse dish etc. that collapses.Purslane originates in temperate zone and torrid areas, except that extremely frigid zones, distribution is arranged all over the world, is that the typical case of China's integration of drinking and medicinal herbs culture embodies.Not only have abundant nutritive value, but also have antibiotic, reducing blood-fat, anti-oxidant, strengthening immunity, multiple pharmacological function such as antitumor, polysaccharide is one of four big base substances that constitute life, has important biological.Because in recent years the purslane flavones is had more research report, Polysaccharide from Portulaca oleracea is rarely reported.

Summary of the invention:

The purpose of this invention is to provide a kind of Polysaccharide from Portulaca oleracea production technique, can access the Polysaccharide from Portulaca oleracea of higher degree through production technique of the present invention.

Technical solution of the present invention is as follows:

The production technique of Polysaccharide from Portulaca oleracea, wherein: production technique is as follows: get purslane, segment, drying are cleaned in the removal of impurity; Soaked 30 minutes, and adopted microwave-assisted to extract 3 times; Under vacuum heating conditions, be evaporated to purslane medicinal extract, add small amount of ethanol, stirring, mixing left standstill taking precipitate 24 hours; Throw out adds the suitable quantity of water dilution, adds chloroform and propyl carbinol and mixes the half an hour of vibrating; To remove proteic purslane Crude polysaccharides and dilute, add wash-out in the macroporous adsorbent resin with suitable quantity of water; In the polymeric amide elutriant, add charcoal absorption, remove gac; Add hydrogen peroxide mixing, decolouring again, residual hydrogen peroxide is removed in heating; Get the Polysaccharide from Portulaca oleracea of higher degree through vacuum-drying.

The invention has the advantages that: the Polysaccharide from Portulaca oleracea of producing can improve immunologic function, and is antitumor, anti-oxidant, anti-ageing, blood sugar reducing function and change diabetic symptom.Compare with other vegetable polysaccharides, the anti-tumor capacity of Polysaccharide from Portulaca oleracea is imitated strong, can be used as the antitumor drug exploitation.

Embodiment

The production technique of embodiment 1, Polysaccharide from Portulaca oleracea, wherein: production technique is as follows:

(1) get purslane, segment, drying are cleaned in the removal of impurity;

(2) soak 30 minutes, adopt microwave-assisted to extract 3 times;

(3) under vacuum heating conditions, be evaporated to purslane medicinal extract, add small amount of ethanol, stirring, mixing left standstill taking precipitate 24 hours; Throw out adds the suitable quantity of water dilution, adds chloroform and propyl carbinol and mixes the half an hour of vibrating;

(4) will remove proteic purslane Crude polysaccharides and dilute, add wash-out in the macroporous adsorbent resin with suitable quantity of water;

(5) in the polymeric amide elutriant, add charcoal absorption, remove gac; Add hydrogen peroxide mixing, decolouring again, residual hydrogen peroxide is removed in heating; Get the Polysaccharide from Portulaca oleracea of higher degree through vacuum-drying.

Claims (1)

1. the production technique of Polysaccharide from Portulaca oleracea, it is characterized in that: production technique is as follows:

(1) get purslane, segment, drying are cleaned in the removal of impurity;

(2) soak 30 minutes, adopt microwave-assisted to extract 3 times;

(3) under vacuum heating conditions, be evaporated to purslane medicinal extract, add small amount of ethanol, stirring, mixing left standstill taking precipitate 24 hours; Throw out adds the suitable quantity of water dilution, adds chloroform and propyl carbinol and mixes the half an hour of vibrating;

(4) will remove proteic purslane Crude polysaccharides and dilute, add wash-out in the macroporous adsorbent resin with suitable quantity of water;

(5) in the polymeric amide elutriant, add charcoal absorption, remove gac; Add hydrogen peroxide mixing, decolouring again, residual hydrogen peroxide is removed in heating; Get the Polysaccharide from Portulaca oleracea of higher degree through vacuum-drying.