CN102240059A - Method for preparing bacteriostat and application of acorn extract used as bacteriostat - Google Patents
Method for preparing bacteriostat and application of acorn extract used as bacteriostat Download PDFInfo
- Publication number
- CN102240059A CN102240059A CN2011101264376A CN201110126437A CN102240059A CN 102240059 A CN102240059 A CN 102240059A CN 2011101264376 A CN2011101264376 A CN 2011101264376A CN 201110126437 A CN201110126437 A CN 201110126437A CN 102240059 A CN102240059 A CN 102240059A
- Authority
- CN
- China
- Prior art keywords
- acorn
- extract
- concentrate
- bacteriostat
- bacteriostatic agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
Abstract
The invention discloses a method for extracting a bacteriostat and application of an acorn extract used as the bacteriostat. The method comprises the following steps of: washing fresh acorns and removing impurities; drying at the drying temperature of between 40 and 80 DEG C, and grinding to obtain acorn powder; adding into solution of methanol, leaching, and refluxing in water bath to obtain leaching liquor; concentrating the leaching liquor to obtain concentrated solution; and adding the concentrating solution into extract liquor and extracting to remove fat, continuing to concentrate in boiling water bath after degreasing until a concentrate is dry to obtain paste, adding solution of acetone into paste and dissolving, extracting for 2 to 4 times, filtering, mixing filtrate, and concentrating and drying to obtain the acorn extract bacteriostat. The acorn extract prepared by the method has remarkable effects of inhibiting Staphylococcus aureus and Escherichia coli. The bacteriostat can be used for corrosion prevention and bacteriostasis of foods and medicines.
Description
Technical field
The present invention relates to a kind of preparation method of food additives, is a kind of preparation method of bacteriostatic agent specifically, also relates to the application of acorn extract as bacteriostatic agent.
Background technology
Acorn is the general name of Fagaceae vegetable seeds.Study of Acorn Resources in China is abundant, and is various in style, produces acorn 60～7,000,000,000 kg per year.Every acorn mature period is seen everywhere in the central mountain Songshan, and the acorn that comes off naturally causes the significant wastage of resource.At present, acorn is except that being used for weaving, extraction tannin extract and making bean curd on a small quantity, and major part is not utilized effectively.
Anticorrisive agent can be divided into natural antiseptic agent and synthetic preservative two big classes according to the source.Most synthetic preservative is unfavorable to human body, as benzoic acid, just has antimicrobial spectrum narrow, and taste is not good, and edible for a long time or excessive amount is to shortcomings such as health cause damage.Characteristics such as natural antiseptic agent has safety non-toxic, and antibiotic property is good have become focus about the research of natural antiseptic agent.
Natural antiseptic agent is mainly derived from plant, animal and microorganism, wherein derive from the maximum of plant, and antibiotic property is better.The number of applying for a patent a kind of preparation method of Bitter Melon P.E bacteriostatic agent that has been 200810147316.8 patent disclosure, this anticorrisive agent fungistatic effect is better, but cost is higher.The invention provides a kind of is the broad-spectrum antiseptic of raw material with the acorn, low cost, and bacteriostatic agent preparation method simple to operate, and with the application of acorn extract in bacteriostatic agent.
Summary of the invention
The invention provides the application as bacteriostatic agent of a kind of preparation method of bacteriostatic agent and acorn extract, is raw material with the acorn, the products material wide material sources, and extracting method is simple to operate, can reduce cost.
It is raw material that the present invention adopts acorn, and its concrete steps are
(1) fresh acorn is cleaned impurity elimination;
(2) when bake out temperature is 40 ℃-80 ℃, dry, be milled to the acorn powder;
(3) add and to carry out carrying out after the lixiviate water-bath in the methanol solution and reflux and obtain leaching liquor;
(4) after being concentrated, leaching liquor obtains concentrate;
(5) concentrate is added extract in the extract and sloughs fat,
(6) in boiling water bath, continue to be concentrated into the dried paste that obtains after the degreasing,
(7) paste is added the acetone soln dissolving, extract 2-4 time, filter, merging filtrate, concentrate drying promptly get the acorn extract, and this acorn extract is bacteriostatic agent.
Described acorn is the general name of Fagaceae vegetable seeds, comprises chinquapin, Mao Li, little red evergreen chinquapin, cork oak, bitter sweet oak, struggle against lithocarpus glaber, southeast lithocarpus glaber, U.S. leaf lithocarpus glaber, the lithocarpus glaber that comes into leaves, Duo Mai Qinggang, spire Qinggang, Da Ye Qinggang firmly.
Acorn is starch-containing mostly to be 50%～70% greatly, soluble sugar 2%～15%, tannin 0.26%～17.74%, protein 1.17%～8.72%, grease 1.04%～6.86%, crude fibre 1.13%～5.89%, ash content 1.30%～3.40%.Wherein lithocarpus glaber genus and Castanea content of starch are higher, can reach 60%～80%; Tannin content belongs to the highest with oak, most kinds reach more than 10%; Grease is rich with the beech in Quercus acutissima, tobacco pipe lithocarpus glaber, Guilin evergreen chinquapin and the Cyclobalanopsis, bright leaf beech equal size, reaches 14.79%, 17%, 20%, 40%～46% and 49% respectively; Some kind vitamin B2 content is abundant, is higher than 7～10 times in grain, is respectively 54.2,54.2 and 63.3 μ g/g as Cortex Querci franchetii, yellow back of the body Qinggang and Qinggang.In addition, rubber benevolence also contains the materials such as five carbon glycan, vitamin B1, vitamin C, Yan acid, pigment, mineral matter, datiscetin and carbohydrate thereof of some.
Tannin is tannic acid or tannic acid again, is divided into Hydrolysable Tannins (HT) and condensed tannin (CT).That tannin has is anti-oxidant, conjugated protein, anticorrosion, in conjunction with polysaccharide, antibacterial and function such as preserve moisture.Because the acorn raw material sources are extensive, utilization rate is low, and contains great deal of tannin class material, is used to extract bacteriostatic agent to exploitation wild plant Study of Acorn Resources, and the economy that develops forestry provides a new approach.
The degree of grinding of described acorn powder is the 30-100 order.
The concentration of described methanol solution is 40%-80%, during lixiviate and the solid-to-liquid ratio of acorn powder be 1: 3-1: 15.
The temperature that described water-bath refluxes is 70-90 ℃, and return time is 2h-4h.
Described extract is benzinum, ethyl acetate or dichloromethane solution, and wherein the volume ratio of extract and concentrate is 1: 1.
The volume ratio of described acetone soln and dope is 1: 2-1: 10.
Described filtrate method for concentration is that normal pressure or decompression concentrate, and dry is vacuum drying, and baking temperature is 30-50 ℃.
Also relate to of the application of acorn extract as bacteriostatic agent.
The present invention compared with prior art, with acorn as the raw material that extract bacteriostatic agent, its wide material sources, various in style; This bacteriostatic agent preparation method is simple, and used solvent is all recyclable to be utilized again, and easy to operate, cost is low, is easy to suitability for industrialized production; This bacteriostatic agent fungistatic effect is obvious, nontoxic, environmental friendliness; This bacteriostatic agent scope of application is wide, can be widely used in food, the medicine, and effect is obvious, only needs 3/10000ths to five can reach fungistatic effect.
The specific embodiment
Below by this patent of concrete implementation:
Embodiment 1:
Take by weighing the acorn 50g of 40 ℃ of oven dry, add 80% methanol solution 150mL, in the 250mL round-bottomed flask, filter behind 70 ℃ of water-bath backflow 2h, the leaching liquor vacuum is concentrated into 50mL according to 1: 3 solid-to-liquid ratio.Add isopyknic petroleum ether extraction in the concentrate to slough fat.In boiling water bath, continue to be concentrated into dried after the degreasing, obtain sticky paste.With the paste volume ratio is 1: 2 acetone solution, filters, and repeats 2 times, merges collection filtrate.Filtrate decompression is concentrated into 20mL, and vacuum drying under 50 ℃ of conditions promptly gets the bacteriostatic agent finished product.
Embodiment 2:
Take by weighing the acorn 50g of 80 ℃ of oven dry, add 60% methanol solution 250mL according to 1: 5 solid-to-liquid ratio, in the 500mL round-bottomed flask, 85 ℃ of water-bath backflow 3h repeat 2 times, can suitably reduce methanol usage for the second time, merge and filter, the leaching liquor vacuum is concentrated into 100mL.Add isopyknic petroleum ether extraction in the concentrate to slough fat.In boiling water bath, continue to be concentrated into dried after the degreasing, obtain the sticky paste of brownish black.With the paste volume ratio is 1: 5 acetone solution, filters, and repeats 2 times, merges collection filtrate.Filtrate decompression is concentrated into 20mL, and vacuum drying under 45 ℃ of conditions promptly gets the bacteriostatic agent finished product.
Embodiment 3:
Take by weighing the acorn 50g of 60 ℃ of oven dry, add 40% methanol solution 150mL, in the 250mL round-bottomed flask, filter behind 90 ℃ of water-bath backflow 4h, the leaching liquor vacuum is concentrated into 50mL according to 1: 15 solid-to-liquid ratio.Add isopyknic petroleum ether extraction in the concentrate to slough fat.In boiling water bath, continue to be concentrated into dried after the degreasing, obtain sticky paste.With the paste volume ratio is 1: 10 acetone solution, filters, and repeats 2 times, merges collection filtrate.Filtrate decompression is concentrated into 20mL, and vacuum drying under 30 ℃ of conditions promptly gets the bacteriostatic agent finished product.
In order to improve extraction efficiency, also can adopt microwave or ultrasonic-wave assisted carrying, to reach higher product yield; The dissolving of concentrate also can adopt ethyl acetate or carrene to extract.
Embodiment 4. acorn extracts are as the external bacteriostatic experiment of bacteriostatic agent:
Adopting staphylococcus aureus, Escherichia coli is indicator bacteria.Microorganism fungus kind after will activating respectively with SPSS is made finely dispersed suspension, adjusts concentration, makes thalline or spore concentration be about 10
6Individual/mL, get the adding of 0.10mL bacteria suspension respectively and contain in the aseptic plate of 20mL aseptic culture medium, the spreading rod with sterilization evenly is coated with bacterium liquid then.
The acorn extract bacteria inhibitor finished product that makes in the foregoing description is made into the sample solution of 0.2g/mL, the sterilization filter paper of diameter 5mm is soaked 30min in sample solution, take out the scraps of paper and dry.Filter paper is affixed on the culture dish surface of culture medium, is inverted, cultivated 24 hours for 37 ℃, carry out bacteriostatic experiment, observe filter paper inhibition zone size on every side, the big more fungistatic effect of inhibition zone is good more, inhibition zone thinks that greater than 10mm fungistatic effect is good, and sterile distilled water is a blank.Every kind of bacterium is cooked three parallel laboratory tests, and record inhibition zone size is averaged, and measurement data is as shown in the table.
The acorn extract supplies examination bacterium fungistatic effect to two kinds
Experimental result shows that the acorn extract is obvious to two kinds of fungistatic effects for the examination bacterium, illustrates that the acorn extract is a kind of good natural bacteriostatic agent.
Claims (9)
1. method for preparing bacteriostatic agent is characterized in that: adopting acorn is the raw material preparing bacteriostatic agent, and its concrete steps are:
(1) fresh acorn is cleaned impurity elimination;
(2) when bake out temperature is 40 ℃-80 ℃, dry, be milled to the acorn powder;
(3) add and to carry out carrying out after the lixiviate water-bath in the methanol solution and reflux and obtain leaching liquor;
(4) after being concentrated, leaching liquor obtains concentrate;
(5) concentrate is added extract in the extract and sloughs fat,
(6) in boiling water bath, continue to be concentrated into the dried paste that obtains after the degreasing,
(7) paste is added the acetone soln dissolving, extract 2-4 time, filter, merging filtrate, concentrate drying promptly get the acorn extract, and this acorn extract is bacteriostatic agent.
2. method according to claim 1, it is characterized in that: described acorn is the general name of Fagaceae vegetable seeds, comprises chinquapin, Mao Li, little red evergreen chinquapin, cork oak, bitter sweet oak, struggle against lithocarpus glaber, southeast lithocarpus glaber, U.S. leaf lithocarpus glaber, the lithocarpus glaber that comes into leaves, Duo Mai Qinggang, spire Qinggang, Da Ye Qinggang firmly.
3. method according to claim 1 is characterized in that: the degree of grinding of described acorn powder is the 30-100 order.
4. method according to claim 1 is characterized in that: the concentration of described methanol solution is 40%-80%, during lixiviate and the solid-to-liquid ratio of acorn powder be 1: 3-1: 15.
5. method according to claim 1 is characterized in that: the temperature that described water-bath refluxes is 70-90 ℃, and return time is 2h-4h.
6. method according to claim 1 is characterized in that: described extract is benzinum, ethyl acetate or dichloromethane solution, and wherein the volume ratio of extract and concentrate is 1: 1.
7. method according to claim 1 is characterized in that: the volume ratio of described acetone soln and dope is 1: 2-1: 10.
8. method according to claim 1 is characterized in that: described filtrate method for concentration is that normal pressure or decompression concentrate, and dry is vacuum drying, and baking temperature is 30-50 ℃.
9. the acorn extract is as the application of bacteriostatic agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110126437 CN102240059B (en) | 2011-05-16 | 2011-05-16 | Method for preparing bacteriostat and application of acorn extract used as bacteriostat |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110126437 CN102240059B (en) | 2011-05-16 | 2011-05-16 | Method for preparing bacteriostat and application of acorn extract used as bacteriostat |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102240059A true CN102240059A (en) | 2011-11-16 |
| CN102240059B CN102240059B (en) | 2013-06-26 |
Family
ID=44958443
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110126437 Expired - Fee Related CN102240059B (en) | 2011-05-16 | 2011-05-16 | Method for preparing bacteriostat and application of acorn extract used as bacteriostat |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102240059B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102584916A (en) * | 2011-12-21 | 2012-07-18 | 浙江大学 | Lignan triglucoside compound and preparation method and application thereof |
| CN108214774A (en) * | 2018-01-08 | 2018-06-29 | 西北农林科技大学 | A kind of softwood sources timber preservative and preparation method thereof |
| CN108904625A (en) * | 2018-07-26 | 2018-11-30 | 上海沃必盛生物科技有限公司 | A kind of pure plant children disinfectant and its application method |
| CN112425711A (en) * | 2020-11-23 | 2021-03-02 | 东北林业大学 | Acorn shell extract coating preservative and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1137530A (en) * | 1996-04-26 | 1996-12-11 | 泰安市泰山林场 | Method for extracting edible starch from acorn and its usage |
| CN101747990A (en) * | 2008-12-15 | 2010-06-23 | 胡景波 | Preparation method of acorn oil |
-
2011
- 2011-05-16 CN CN 201110126437 patent/CN102240059B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1137530A (en) * | 1996-04-26 | 1996-12-11 | 泰安市泰山林场 | Method for extracting edible starch from acorn and its usage |
| CN101747990A (en) * | 2008-12-15 | 2010-06-23 | 胡景波 | Preparation method of acorn oil |
Non-Patent Citations (3)
| Title |
|---|
| 张濛等: "橡子酚抗菌洗剂对淋病奈瑟菌和解脲支原体的抑菌效果", 《中国消毒学杂志》 * |
| 胡芳名等: "湖南省主要橡子资源综合开发利用的研究", 《中南林学院学报》 * |
| 黄利群等: "橡子研究概况", 《氨基酸和生物资源》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102584916A (en) * | 2011-12-21 | 2012-07-18 | 浙江大学 | Lignan triglucoside compound and preparation method and application thereof |
| CN102584916B (en) * | 2011-12-21 | 2014-01-15 | 浙江大学 | Lignan triglucoside compound and preparation method and application thereof |
| CN108214774A (en) * | 2018-01-08 | 2018-06-29 | 西北农林科技大学 | A kind of softwood sources timber preservative and preparation method thereof |
| CN108904625A (en) * | 2018-07-26 | 2018-11-30 | 上海沃必盛生物科技有限公司 | A kind of pure plant children disinfectant and its application method |
| CN112425711A (en) * | 2020-11-23 | 2021-03-02 | 东北林业大学 | Acorn shell extract coating preservative and preparation method thereof |
| CN112425711B (en) * | 2020-11-23 | 2023-01-24 | 东北林业大学 | Acorn shell extract coating preservative and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102240059B (en) | 2013-06-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102273715B (en) | Common lophatherum herb extract natural food preservative and preparation method thereof | |
| CN101647474B (en) | Plant-derived bacteriostatic extractive and application thereof | |
| CN107557224B (en) | Hovenia acerba and Chinese wolfberry health wine and brewing method thereof | |
| Basso et al. | Chemical features and antioxidant profile by Schizophyllum commune produced on different agroindustrial wastes and byproducts of biodiesel production | |
| CN102240059B (en) | Method for preparing bacteriostat and application of acorn extract used as bacteriostat | |
| CN103461693A (en) | Forage for big pig, and preparation method thereof | |
| CN103766608A (en) | Eucalyptus leaf extractive natural meat quality improver and preparation method thereof | |
| CN103468019A (en) | Natural pigment prepared by using fruit residues and applications thereof | |
| Meenashree et al. | Evaluation of total phenolic content and antimicrobial activities exhibited by the leaf extracts of Musa acuminata (banana) | |
| CN109362967A (en) | It is a kind of to alleviate pig oxidative stress, the plant extract feed additive of improvement meat and application | |
| CN105580722A (en) | Culture medium suitable for tea-oil trees | |
| CN104171325A (en) | Animal feed | |
| CN104488977A (en) | Chinese ash bark and fruit extractive and novel application thereof | |
| CN103099291A (en) | Preparation method of natural preservative regarding red bayberry leaves as raw material | |
| CN101152018A (en) | Method for producing natural antiseptic agent midbody with blueberry leaf and uses thereof | |
| CN104336106A (en) | Natural biomass compound preservative and preparation method thereof | |
| CN104119137A (en) | Flammulina velutipes culture medium prepared from mushroom residue and preparation method thereof | |
| CN104622732B (en) | A kind of hair washing for child water containing natural plant preservative | |
| CN110574926A (en) | process for high-value utilization of innominate sunflower | |
| CN102860494B (en) | Polysaccharide and flavoring material from Coprinus comatus fruiting bodies and preparation method of polysaccharide and flavoring material | |
| CN110205091B (en) | Plant-source antibacterial and mildew-proof environment-friendly wallpaper adhesive and preparation method thereof | |
| CN107027829A (en) | A kind of tea tree compound botanical source pesticide with synergistic effect | |
| CN107333874A (en) | A kind of peach preservation method | |
| CN103087922B (en) | Penicillium, cellulose produced by fermenting penicillium in solid state and method thereof for preparing pomelo peel high-ester pectin | |
| CN102210831A (en) | Compound smartweed preparation and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130626 Termination date: 20140516 |