CN102229896A - Application of radiation resistant bacteria in biological treatment of heavy metal ions - Google Patents
Application of radiation resistant bacteria in biological treatment of heavy metal ions Download PDFInfo
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- CN102229896A CN102229896A CN2010101666670A CN201010166667A CN102229896A CN 102229896 A CN102229896 A CN 102229896A CN 2010101666670 A CN2010101666670 A CN 2010101666670A CN 201010166667 A CN201010166667 A CN 201010166667A CN 102229896 A CN102229896 A CN 102229896A
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Abstract
The invention discloses an application of a radiation resistant bacteria Deinococcus wushiensis CGMCC No. 1.8884 in the biological treatment of heavy metal ions. The invention is characterized by: inoculating the bacterial strain Deinococcus wushiensis CGMCC No. 1.8884 to a liquid medium filled with 5ml TGY for cultivation at the temperature of 30 DEG C, carrying out a 200rpm shake cultivation for 36h, then cultivating according to an inoculation amount of 2%. The bacterial strain used in the invention has tolerance to Hg2+, Cu2+, Co2+, Pb2+ and Cr2+, wherein, the tolerance to Pb2+ and Cr2+ can reach to 150mg/L and 100mg/L respectively, and the tolerance to Cu2+ and Co2+ can reach to 80mg/L. The invention can be widely used in the field of environmental protection treatment of heavy metal waste liquor.
Description
Invention field
The present invention relates to heavy metal biological adsorption technology field, concrete, the present invention relates to the technical field that a kind of radiation hardness bacterium is used in heavy metal ion adsorbed.
Background technology
Heavy metal is meant that proportion is greater than 4 or 5g/cm
3Metal, have 45 kinds approximately, as copper, zinc, lead, cobalt, manganese, cadmium, mercury etc.Because it is human increasing to exploitation, smelting, processing and the commercial manufacturing activities of heavy metal, cause various heavy to enter in atmosphere, water, the soil as lead, mercury, cadmium, copper, cobalt etc., along with heavy metal ion retains, accumulates and move in the environment or the ecosystem, cause serious environmental to pollute and ecological hazard.
The physico-chemical process of conventional process heavy metal-containing waste water comprises chemical precipitation method, ion exchange method, electrolytic process, coacervation and oxidation reduction process etc., and these methods are greatly expensive and may cause secondary pollution.In recent years, biosorption process, advantages of being cheap efficient with it caused environmentalist's attention gradually.Compare with traditional treatment process, biosorption process is to pass through organism, mainly comprise the adsorption of bacterium, fungi, algae and agriculture and forestry organic waste material and derivative thereof to heavy metal ion in the water, reach the purpose of removing and reclaiming heavy metal, compare with traditional sorbent material, they have wide adaptability, can operate under different pH, temperature and the course of processing; Concentration of metal ions influence is little, lower concentration (＜10mg/L) and high density (＞the good metal adsorptive power is all arranged under 100mg/L); Regenerative power is strong, step is simple, and regeneration back adsorptive power does not have advantages such as obvious reduction.The biology absorption of various heavy is studied both at home and abroad at present, all received good effect.
In heavy multi-metal, cobalt metal (Co) is as a kind of important strategic metal, be the important source material of making superalloy, Wimet, magneticalloy, Precise Alloy and cobalt compound, be widely used in that aerospace, motor are electric, mechanical, industries such as chemical industry, pottery, communication and battery; Cobalt element generally extensively is present in occurring in nature with natural concentration, because human in recent years increasing to its exploitation, smelting, processing and commercial manufacturing activities, particularly contains arbitrarily abandoning of cobalt store battery, and as the isotropic substance Co of radiation sterilization
60Improper use and leakage cause cobalt element to enter in atmosphere, water, the soil with various chemical states or chemical form, by retaining, accumulate and moving, have not only caused the serious environmental pollution, also cause the loss of noble metal cobalt simultaneously.
Processing contains cobalt waste liquid main method at present employing sulfurization-precipitation method [Chen Weiping, Yang Qingshan, He Bining, recovery research Deng cobalt in the .NdFeB waste material. rare metal and Wimet, 2006,34:55-57], the ammonium oxalate precipitator method [Tan Haixiang, Hu Qiyang, Li Xinhai, Deng. cobalt reclaims novel process in the useless pole piece of cobalt acid lithium. power technology, 2007,31:288-230], the hydrochloric acid back extraction method [handsome state power. the recovery of Jinchuan company cobalt. nonferrous smelting, method such as 1995 (3): 15-18], these treatment processs have obtained good effect to a certain extent, but the ubiquity secondary pollution, particularly when the heavy metal concentration in the water lower (＜100mg/L) time, not only clearance is lower, and working cost is higher.At present, still there is not adsorption of cobalt ionic relevant report.
The processing that contains the heavy metal waste liquid is not only helped environment protection, also can accomplish to reclaim simultaneously the purpose of important strategic metal.Utilize biological adsorption treatment be a kind of simple to operate, effective, less investment, operating costs low, be easy to manage and the method for environmental protection.
Summary of the invention:
At still there not being adsorption of cobalt ionic relevant report at present, particularly there be not the application of bacterial strain Deinococcuswushiensis in the heavy metal ion biological treatment.The object of the present invention is to provide a kind of deinococcus radiodurans that utilizes to carry out application in the heavy metal ion biological treatment, particularly the invention provides a kind of application of deinococcus radiodurans in the biological treatment of adsorption of cobalt ion that utilize.
The present invention specifically provides a kind of deinococcus radiodurans Deinococcus wushiensis CGMCCNo.1.8884 that utilizes to carry out utilisation technology scheme in the heavy metal ion biological treatment.The used bacterial strain of the present invention is to Hg
2+, Cu
2+, Co
2+, Pb
2+, Cr
2+Five kinds of ions all have resistance characteristics, wherein to Pb
2+, Cr
2+Tolerance concentration maximum, can reach 150mg/L and 100mg/L respectively; Right ' Cu
2+, Co
2+Tolerance take second place, can reach 80mg/L; To Hg
2+Tolerance minimum, at 30mg/L.
Microorganism strains Deinococcus wushiensis CGMCC No.1.8884 used in the present invention, by Microorgan Application Inst., Xinjiang Agricultural Academy's separation screening, be preserved in the international depositary institution of budapest treaty microorganism: China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC).Address: Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode: 100101, preservation date is on May 12nd, 2009, culture presevation number is CGMCC No.1.8884, and the biology specific name is Deinococcus wushiensis, and it is typical anti-gamma-radiation bacterial classification.20-35 ℃ of this strain culturing temperature, about 30 ℃ of the suitableeest culture temperature.(peptone 5g, yeast extract paste 3g, glucose 2g, agar 15g, distilled water IL pH7.5), cultivate through 30 ℃, 48h, and that bacterium colony is is orange red, little, circular, neat in edge, smooth surface, opaque in the TGY media surface in this growth; The cell circle, be bigeminy or tetrad shape.
In bacteria culture medium, preferred TGY liquid nutrient medium, is cultivated 24～48h at 180～200rpm, 30 ℃ with the inoculation of multiplication culture; The culture that fermentation culture obtained is through the centrifugal collection thalline of 4000rpm.Method by 50 ℃ of oven dry obtains dry mycelium.The 0.1g dry mycelium is added in the cobalt ion solution of pH 5.0, concentration 50mg/L, agitation as appropriate, room temperature is adsorbed 30min, can reach the purpose of cobalt in the adsorbent solution, and the maximum adsorption rate reaches 84.5%.
By implementing the concrete technical indicator of the present invention, can reach following beneficial effect.
The application of Deinococcus wushiensis CGMCC No.1.8884 provided by the invention in heavy metal ion adsorbed, Deinococcus wushiensis CGMCC No.1.8884 is to Hg
2+, Cu
2+, Co
2+, Pb
2+, Cr
2+Five kinds of ions all have resistance characteristics, wherein to Pb
2+, Cr
2+Tolerance concentration maximum, can reach 150mg/L and 100mg/L respectively; To Cu
2+, Co
2+Tolerance take second place, can reach 80mg/L; To Hg
2+Tolerance minimum, at 30mg/L.Can utilize the growth adsorption of cobalt ion of thalline by this law, also can directly use dry mycelium absorption, the maximum adsorption ability can reach the 44.5mg/g dry mycelium.
Description of drawings:
Fig. 1: bacterial strain Deinococcus wushiensis CGMCC No.1.8884 is to different metal ionic tolerance.
Fig. 2: bacterial strain Deinococcus wushiensis CGMCC No.1.8884 is at 50mg/L Co
2+Growth curve and characterization of adsorption under the pressure.
Fig. 3: different solutions pH is to the influence of Deinococcus wushiensis CGMCC No.1.8884 dry mycelium absorption.
Fig. 4: different concns Co
2+Influence to the absorption of Deinococcus wushiensis CGMCC No.1.8884 dry mycelium.
Fig. 5: different adsorption times are to the influence of Deinococcus wushiensis CGMCC No.1.8884 dry mycelium absorption
Embodiment:
Below be specific embodiments of the invention, but the present invention is not limited thereto.
Embodiment 1: the liquid culture of bacterial strain Deinococcus wushiensis CGMCC No.1.8884
Activated bacterial strain Deinococcus wushiensis of the present invention is inoculated in the seed test tube that 5ml TGY liquid nutrient medium is housed, in 30 ℃ of cultivations, behind the 200rpm shaking culture 36h, be inoculated in (amount of adding is the bottled 80ml TGY of a 500ml triangle liquid nutrient medium) in the TGY liquid fermenting bottle by 2% inoculum size, in 30 ℃ of cultivations, 220rpm shaking culture 60h.
After above-mentioned cultivation, the thalline peak concentration can reach 4.51OD/ml (600nm).Culture is centrifugal through 8000rpm, 3min, abandons supernatant, collects thalline, and the thalline weight in wet base can reach 5.2g/L.
Embodiment 2: the anti-heavy metal characteristic of bacterial strain Deinococcus wushiensis CGMCC No.1.8884
Activated bacterial strain Deinococcus wushiensis of the present invention is inoculated in the seed test tube that 5ml TGY liquid nutrient medium is housed,, behind the 200rpm shaking culture 36h, is inoculated in the Hg that contains different concns respectively by 2% inoculum size in 30 ℃ of cultivations
2+, Cu
2+, Co
2+, Pb
2+, Cr
2+In the TGY liquid fermenting bottle (amount of adding is the bottled 80ml TGY of a 500ml triangle liquid nutrient medium), in 30 ℃ of cultivations, 220rpm shaking culture 72h, the growing state of observation bacterial classification.The result is referring to accompanying drawing 1, by accompanying drawing 1 as can be seen this patent bacterial strain Deinococcus wushiensis multiple metal is had tolerance, Pb wherein
2+, Cr
2+Tolerance concentration maximum (100mg/L) Cu
2+, Co
2+(80mg/L) takes second place.
Embodiment 3: bacterial strain Deinococcus wushiensis CGMCC No.1.8884 is at different Co
2+Growth characteristics under the concentration
Activated bacterial strain Deinococcus wushiensis of the present invention is inoculated in the seed test tube that 5ml TGY liquid nutrient medium is housed, in 30 ℃ of cultivations, behind the 200rpm shaking culture 36h, be inoculated in by 2% inoculum size contain 0,20,50 respectively, 80mg/L Co
2+TGY liquid fermenting bottle in (amount of adding is the bottled 80ml TGY of a 500ml triangle liquid nutrient medium), in 30 ℃ of cultivations, 220rpm shaking culture 120h, and every 12h timing sampling is measured thalline OD value, the growing state of observation bacterial classification.The result shows, the Co of lower concentration
2+(＜20mg/L) is to the no obvious suppression of bacterial strain Deinococcus wushiensis growth, and after concentration was greater than 50mg/L, strain growth obviously was suppressed (seeing Table 1).Referring to accompanying drawing 2.
Table 1 different concns Co
2+Influence to strain growth
Embodiment 4:Deinococcus wushiensis CGMCC No.1.8884 growth absorption Co
2+Characteristic and effect
Activated inoculation of the present invention in the seed test tube that 5ml TGY liquid nutrient medium is housed, in 30 ℃ of cultivations, behind the 200rpm shaking culture 36h, is inoculated in by 2% inoculum size and contains 50mg/L Co respectively
2+TGY liquid fermenting bottle in (amount of adding is the bottled 80ml TGY of a 500ml triangle liquid nutrient medium), in 30 ℃ of cultivations, 220rpm shaking culture 120h, and every 12h timing sampling is measured Co in thalline OD value and the fermented liquid
2+Content, the result is referring to accompanying drawing 2.By accompanying drawing 2 as can be seen bacterial strain Deinococcus wushiensis can self the growth in active adsorption Co
2+, its adsorption rate is 85.3%.
Embodiment 5: the preparation of bacterial strain Deinococcus wushiensis CGMCC No.1.8884 dry mycelium
Activated bacterial strain Deinococcus wushiensis of the present invention is inoculated in the seed test tube that 5ml TGY liquid nutrient medium is housed, in 30 ℃ of cultivations, behind the 200rpm shaking culture 36h, be inoculated in (amount of adding is the bottled 80ml TGY of a 500ml triangle liquid nutrient medium) in the TGY liquid fermenting bottle by 2% inoculum size, in 30 ℃ of cultivations, 220rpm shaking culture 48h.
Culture is centrifugal through 8000rpm, 3min, abandons supernatant, collects thalline.Thalline behind deionized water wash 3 times, centrifugal collection thalline, place 60 ℃ of baking ovens to dry to constant weight after, put into dry still and preserve stand-by.
Embodiment 6: different solutions pH is to the influence of Deinococcus wushiensis CGMCC No.1.8884 dry mycelium absorption
Accurately measure the Co that concentration is 50mg/L
2+Among the solution 50ml, use HCl, NaOH to transfer to after pH is 3.0,4.0,5.0,6.0,7.0 respectively, add deionized water and be settled to 100ml, and after using the acidometer calibration, add dry mycelium 0.1g respectively and break up to evenly, behind the room temperature vibration absorption 1h, sampling centrifuging and taking supernatant, and, analyze Co in the filtrate with the filter membrane suction filtration of 45um
2+Concentration, and calculating adsorption rate separately are referring to accompanying drawing 3.Can see Deinococcus wushiensis dry mycelium adsorptive capacity maximum when the pH5.0 by accompanying drawing 3.
Embodiment 7: different concns Co
2+Influence to the absorption of Deinococcus wushiensis CGMCC No.1.8884 dry mycelium
Measure 100mL concentration respectively and be 25,50,75, the Co of 100mg/L
2+Solution (pH 5.0) accurately adds Deinococcus wushiensis dry mycelium 0.10g and breaks up to evenly, behind the room temperature vibration absorption 1h, and sampling centrifuging and taking supernatant, and with the filter membrane suction filtration of 45um, Co in the analysis filtrate
2+Concentration, and calculating adsorption rate and unit adsorptive capacity separately, the result is referring to accompanying drawing 4.Can see that by accompanying drawing 4 Deinococcuswushiensis dry mycelium adsorption rate descends along with the increase of concentration, but the unit adsorptive capacity tends towards stability in concentration during more than or equal to 50mg/L, illustrate that under these conditions optimum concn is 50mg/L.
Embodiment 8: different adsorption times are to Deinococcus wushiensis CGMCC No.1.8884 dry mycelium absorption Co
2+Influence
Accurately take by weighing RUV-113 dry mycelium 0.10g respectively, placing concentration is the Co of 50mg/L
2+Among solution (pH 6.5) 100ml, it is centrifugal to take a sample after room temperature was vibrated 0,10,20,30,40 minute respectively, gets supernatant also with the filter membrane suction filtration of 45um, analyzes Co in the filtrate
2+Concentration, and calculating adsorption rate separately are referring to accompanying drawing 5.By accompanying drawing 5 as can be seen, adsorb 30min under these conditions, absorption reaches balance substantially, and the maximum adsorption rate is 84.5%, and reaching adsorptive power most is the 44.5mg/g dry mycelium.Measurement result is as shown in table 2.
Table 2 Deinococcus wushiensis dry mycelium is to the attached effect of telling of other metal ion
Claims (5)
1. deinococcus radiodurans Deinococcus wushiensis CGMCC No.1.8884.
2. one kind is utilized the application of deinococcus radiodurans Deinococcus wushiensis CGMCC No.1.8884 in heavy metal ion adsorbed.
3. the application of deinococcus radiodurans Deinococcus wushiensis CGMCC No.1.8884 as claimed in claim 2 in heavy metal ion adsorbed, it is characterized in that, described deinococcus radiodurans Deinococcuswushiensis CGMCC No.1.8884 is inoculated in the seed test tube that 5ml TGY liquid nutrient medium is housed, in 30 ℃ of cultivations, behind the 200rpm shaking culture 36h, be inoculated in the Hg that contains different concns respectively by 2% inoculum size
2+, Cu
2+, Co
2+, Pb
2+, Cr
2+In the TGY liquid fermentation medium, in 30 ℃ of cultivations, 220rpm shaking culture 72h.
4. the application of a deinococcus radiodurans Deinococcus wushiensis CGMCCNo.1.8884 as claimed in claim 2 in heavy metal ion adsorbed, it is characterized in that described deinococcus radiodurans Deinococcus wushiensis CGMCC No.1.8884 is at absorption Co
2+In application.
5. the application of deinococcus radiodurans Deinococcus wushiensis CGMCC No.1.8884 as claimed in claim 4 in heavy metal ion adsorbed, it is characterized in that described deinococcus radiodurans Deinococcuswushiensis CGMCC No.1.8884 is at absorption Co
2+In application, deinococcus radiodurans Deinococcuswushiensis CGMCC No.1.8884 is inoculated in the seed test tube that 5ml TGY liquid nutrient medium is housed, in 30 ℃ of cultivations, behind the 200rpm shaking culture 36h, be inoculated in Co greater than 50mg/L by 2% inoculum size
2+The TGY liquid nutrient medium, in 30 ℃ of cultivations, 220rpm shaking culture 120h.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103205379A (en) * | 2013-03-26 | 2013-07-17 | 新疆农业科学院微生物应用研究所 | Deinococcus wushiensis and application thereof in cobalt ion biological adsorption treatment |
| CN104694419A (en) * | 2015-02-05 | 2015-06-10 | 浙江大学 | Efficient lead ion bio-adsorbent and preparation method and application thereof |
| CN104785224A (en) * | 2015-04-10 | 2015-07-22 | 合肥环照高分子材料厂 | Agar/kelp powder heavy metal adsorbent and preparation method thereof |
-
2010
- 2010-05-10 CN CN2010101666670A patent/CN102229896A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103205379A (en) * | 2013-03-26 | 2013-07-17 | 新疆农业科学院微生物应用研究所 | Deinococcus wushiensis and application thereof in cobalt ion biological adsorption treatment |
| CN103205379B (en) * | 2013-03-26 | 2014-05-28 | 新疆农业科学院微生物应用研究所 | Deinococcus wushiensis and application thereof in cobalt ion biological adsorption treatment |
| CN104694419A (en) * | 2015-02-05 | 2015-06-10 | 浙江大学 | Efficient lead ion bio-adsorbent and preparation method and application thereof |
| CN104694419B (en) * | 2015-02-05 | 2017-11-17 | 浙江大学 | A kind of efficiently lead ion biological adsorption agent and preparation method and application |
| CN104785224A (en) * | 2015-04-10 | 2015-07-22 | 合肥环照高分子材料厂 | Agar/kelp powder heavy metal adsorbent and preparation method thereof |
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Application publication date: 20111102 |