CN102225180A - Traditional Chinese medicine composition having anticancer effect and preparation method thereof - Google Patents
Traditional Chinese medicine composition having anticancer effect and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition having an anticancer effect and a preparation method thereof. The traditional Chinese medicine composition is a medicament mixture composed of a polyphyllin extract and a turmeric water extract in a mass ratio of 2:3. The preparation process of the traditional Chinese medicine composition comprises the following steps: preparing the polyphyllin extract; preparing the turmeric water extract; and preparing a mixture of the polyphyllin extract and the turmeric water extract. The traditional Chinese medicine composition has the advantages that: the preparation process is simple; the obtained traditional Chinese medicine composition has the anticancer effect, and the anticancer activity of the composition is high and has broad spectrum; and compared with singly applied polyphyllin extract or turmeric water extract, the composition has stronger antitumour activity, smaller toxic and side effects and immunity-enhancing function.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition and preparation method thereof, belong to technical field of Chinese medicines with antitumaous effect.
Background technology
The Chinese medicine Rhizoma Paridis is Liliaceae Paris Linnaeus(Paris L.) (Paris) plant Rhizoma Paridis (Paris polyphylla Smith var.yannanensis) and Rhizoma Paridis (paris polyphylla Smith var.chinensis (Franch.) Hara) rhizome, has heat-clearing and toxic substances removing, the effect of reducing swelling and alleviating pain, cool liver arresting convulsion, being used for the treatment of diseases such as too fat to move, laryngopharynx swelling and pain, venom, traumatic pain, cool breeze tic, is the main composition medicine of famous Chinese patent medicine YUNNAN BAIYAO, jidesheng sheyao tablets, GONGXUENING JIAONANG etc.The modern pharmacology effect shows that this medical instrument has hemostasis, eliminates the phlegm and antibacterial, antalgic and sedative, antiearly pregnancy are killed effects such as sperm, anti-cell poison, in treatment dysfunctional uterine hemorrhage, neurodermatitis and surgery inflammation and treatment tumor etc., significant curative effect is arranged all in clinical practice.The compound recipe that with the Rhizoma Paridis is main component at present has the multiple recovery oral liquid of gold, building lotus capsule, gan fu le capsule etc.Bibliographical information about the antitumor action of Rhizoma Paridis total saponins is more and more.CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2008,33 (16): 2057-2060 discloses cell toxicant spectrum and the structure activity study of Rhizoma Paridis saponin to 10 kinds of tumor cell lines; Wang Yu etc. are at CHINA JOURNAL OF CHINESE MATERIA MEDICA, and 2007,32 (14): 1425-1428) delivered the anti-tumor active ingredient research of Rhizoma Paridis; Cheng Zhixiang etc. study Rhizoma Paridis total saponins effect human hepatoma cell line HepG2's cytotoxic effect and protein science; Observations such as Shi Xiaofeng find that Rhizoma Paridis total saponins aqueous solution filling stomach and lumbar injection all can obviously suppress the growth of tumor-bearing mice tumor tissue, prolong the doubling time of tumor, nucleic acid isotope precursor mixes experiment and shows, Rhizoma Paridis total saponins can disturb the H22 oncocyte to form DNA, RNA, can also disturb the biosynthesis of tumor-bearing mice spleen tissue DNA, RNA simultaneously.Chinese patent 03135589.7,200610052223.8 discloses the preparation method of Rhizoma Paridis total saponins, and 200810052092.2 also to disclose be pharmaceutical preparation of effective ingredient and preparation method thereof with the Rhizoma Paridis total saponins.Rhizoma Curcumae Longae (scutellariae barbatae) is the herb of Labiatae Scutellaria plant Rhizoma Curcumae Longae, the Rhizoma Curcumae Longae all herbal medicine, the property suffering, hardship, cold, go into lung, liver, kidney channel, have effects such as heat-clearing and toxic substances removing, dissipating blood stasis hemostasis, reducing swelling and alleviating pain, be used for the treatment of diseases such as cancer, hepatitis, laryngopharynx swelling and pain, lung abscess, urethritis, dysentery and venom.Carry according to " Chinese oncotherapy great achievement ", Rhizoma Curcumae Longae cures mainly gynecological tumors such as primary hepatocarcinoma etc., digestive tract tumor, pulmonary carcinoma and cervical cancer.Modern study and clinical proof, Rhizoma Curcumae Longae have the effect paid more and more attention of reverse multiple drug resistance of tumor, become the focus of current research.Rhizoma Curcumae Longae contains the number of chemical composition, main component is curcumin and Rhizoma Curcumae Longae volatile oil, they are its antineoplastic effective compositions, according to surveying and determination, turmeric oil content can reach 4.0%-6.0%, curcumin is 1.8%-5.4%, and Rhizoma Curcumae Longae also contains saccharide, stigmasterol, cupreol, fatty acid and trace element etc. in addition.Have only the patent of the pharmaceutical composition (CN101152408A) of Rhizoma Paridis saponin and astragalus polysaccharides to be seen in report and curcumin and chemotherapeutic compatibility at present and be seen in report.Up to the present the Chinese medicine composition of not finding to have antitumaous effect as yet and being made up of Rhizoma Paridis saponin extract and Rhizoma Curcumae Longae water extract is seen in report.
Summary of the invention
The object of the present invention is to provide to the present invention relates to a kind of Chinese medicine composition and preparation method thereof with antitumaous effect, described Chinese medicine composition with antitumaous effect, its active anticancer is high and have broad spectrum activity, and its preparation method is simple.
The present invention is realized by the following technical programs, a kind of Chinese medicine composition with antitumaous effect, it is characterized in that, this Chinese medicine composition with antitumaous effect is by mass ratio 2: 3 by Rhizoma Paridis saponin extract and Rhizoma Curcumae Longae water extract, and to contain 25 milligrams of Rhizoma Paridis saponin extracts altogether in every ml water solution and the Rhizoma Curcumae Longae water extract is formed by compatibility, the quality that wherein contains the steroidal saponins in the Rhizoma Paridis saponin extract is 34～39%, all the other are unidentified saponins still, Rhizoma Curcumae Longae water extract main component is a curcumin, demethoxycurcumin, bisdemethoxycurcumin.
The preparation method of the above-mentioned Chinese medicine composition with antitumaous effect is characterized in that comprising following process:
1) Rhizoma Paridis saponin extracts
(1) the Rhizoma Paridis coarse powder is crossed the 4-6 mesh sieve after, in Rhizoma Paridis powder and the ethanol water 1g of sieving: 4～8ml ratio, the Rhizoma Paridis powder was soaked in the alcoholic solution that mass concentration is 60-80% 1 hour, then 90 ℃ of following heating and refluxing extraction of temperature 2 hours, reclaim extracting solution, and carry out reflux, extract, again 2 times by the 1st reflux, extract, operating condition, merge 3 times extracting solution, extracting solution evaporates down for 80 ℃ in temperature and obtains concentrated solution;
(2) step (1) concentrated solution being added the volume multiple is that 5～10 times mass concentration is to be uniformly dispersed in 1～30% alcoholic solution, and supernatant is collected in centrifugation;
(3) supernatant that step (2) is obtained by the big adsorbent resin chromatographic column of D101, discards effluent with the flow velocity of 1～3 column volume per hour, and the adsorbent resin in the coupled columns carries out eluting then; At first with mass concentration 30%～40% ethanol water with the flow velocity eluting of 1～2 column volume per hour 2～4 hours, reject eluent; And then with mass concentration 70%～85% ethanol water and 1～2 column volume eluting 3～5 hours per hour, collect ethanol elution, to the ethanol elution concentrating under reduced pressure, obtain containing the Rhizoma Paridis saponin extract of the steroidal saponins of mass fraction 34～39% behind the vacuum drying.
2) preparation of Rhizoma Curcumae Longae water extract
(1) curcuma powder is broken into the 1cm fritter, in Rhizoma Curcumae Longae fritter and deionized water 1g: 8～10ml ratio adds Rhizoma Curcumae Longae in the deionized water, extracts half an hour for 100 ℃ in temperature, reclaims extracting solution, and carry out reflux, extract, again 2 times by the 1st reflux, extract, operating condition, merge 3 times extracting solution;
(2) extracting solution that step 1) is merged after filtration, filtrate concentrates with rotary evaporator, concentrated solution obtains being the Rhizoma Curcumae Longae water extract in 100 ℃ of evaporation dryings of temperature, quality is the 10-12% of Rhizoma Curcumae Longae quality;
3) Rhizoma Paridis saponin extract and Rhizoma Curcumae Longae water extract combination
Press the mass ratio 2: 3 of Rhizoma Paridis saponin extract powder and Rhizoma Curcumae Longae water extract, add in the deionized water, obtain having the Chinese medicine composition of antitumaous effect through ultrasonic mix homogeneously, to contain 25 milligrams of Rhizoma Paridis saponin extracts and Rhizoma Curcumae Longae water extract in every ml water solution altogether.
The invention has the advantages that use separately with Rhizoma Paridis saponin and compare, tumour inhibiting rate obviously improves.And this Chinese medicine composition preparation method is simple, and activity is higher and effect is obvious.Compare with the astragalus polysaccharides pharmaceutical composition with Rhizoma Paridis saponin in the past and to have broad spectrum anticancer, at multiple cancer, hepatocarcinoma, Emhorn, the S180 sarcoma all has good therapeutic effect, and inhibitory rate is to 40%-50%.
The specific embodiment
Embodiment one:
1) Rhizoma Paridis saponin extracts
(1) will cross Rhizoma Paridis coarse powder 500g behind 4 mesh sieves, the coarse powder that sieves is soaked in the alcoholic solution 1 hour of 2 mass per liter concentration 60%, then 90 ℃ of following heating and refluxing extraction of temperature 2 hours, reclaim 1.8 liters of extracting solution, and carry out reflux, extract, again 2 times by the 1st reflux, extract, operating condition, after merging 5.4 liters of 3 extracting solution, in the evaporation and obtain concentrated solution down of 80 ℃ of thickening temperatures, volume is 0.5L.
(2) mass concentration with step (1) 0.5L concentrated solution adding 5L is to be uniformly dispersed in 1% alcoholic solution, and centrifugation gets supernatant 4.8L.
(3) be 0.8L with the flow velocity of 2.4L per hour by column volume with the 4.8L supernatant, blade diameter length ratio is the big adsorbent resin chromatographic column of 1: 5 D101, discards effluent; At first with mass concentration 30% ethanol water with the flow velocity eluting of 0.8L per hour 3 hours, reject eluent; With mass concentration 70% ethanol water with the flow velocity eluting of 0.gL per hour 3 hours, collect ethanol elution then, can obtain containing steroidal saponins mass fraction 34% Rhizoma Paridis saponin extract 15g behind the concentrating under reduced pressure vacuum drying.
2) preparation Rhizoma Curcumae Longae water extract
(1) Rhizoma Curcumae Longae 200g is ground into the 1cm fritter, adds 1.6 liters of deionized waters, extract half an hour for 100 ℃, reclaim extracting solution, and carry out reflux, extract, again 2 times, merge 4.6 liters of 3 extracting solution by the 1st reflux, extract, operating condition in temperature;
(2) extracting solution that step 1) is obtained concentrates with Rotary Evaporators after filtration, and 100 ℃ of evaporation dryings obtain being Rhizoma Curcumae Longae water extract 20g that quality is 10% of a Rhizoma Curcumae Longae quality.
(3) get Rhizoma Paridis saponin extract powder 10g and Rhizoma Curcumae Longae water extract 15g, be mixed with the Chinese medicine composition mixed liquor that contains 25 grammes per square metre building saponin extracts and Rhizoma Curcumae Longae water extract in every premium on currency solution altogether with antitumaous effect.Embodiment two:
1) Rhizoma Paridis saponin extracts
(1) will cross Rhizoma Paridis coarse powder 500g behind 4 mesh sieves, the coarse powder that sieves is soaked in the alcoholic solution 1 hour of 2.5 mass per liter concentration 70%, then 90 ℃ of following heating and refluxing extraction of temperature 2 hours, reclaim 2.3 liters of extracting solution, and carry out reflux, extract, again 2 times by the 1st reflux, extract, operating condition, after merging 6.9 liters of 3 extracting solution, in the evaporation and obtain concentrated solution down of 80 ℃ of thickening temperatures, volume is 0.5L.
(2) it is to be uniformly dispersed in 10% alcoholic solution that the 0.5L concentrated solution that step (1) is obtained adds the 4L mass concentration, and centrifugation gets supernatant 3.9L.
(3) be 800mL with the flow velocity of 1.6L per hour by column volume with the 3.9L supernatant, blade diameter length ratio is the big adsorbent resin chromatographic column of 1: 5 D101, discards effluent; At first with mass concentration 35% ethanol water with the flow velocity eluting of 1.6L per hour 4 hours, reject eluent; With mass concentration 75% ethanol water with the flow velocity eluting of 1.6L per hour 4 hours, collect ethanol elution then, can obtain containing the Rhizoma Paridis saponin extract 15g of steroidal saponins mass fraction 36% behind the concentrating under reduced pressure vacuum drying.
2) preparation Rhizoma Curcumae Longae water extract
(1) Rhizoma Curcumae Longae 200g is ground into the 1cm fritter, adds deionized water 1.8L, extracted 1 hour for 100 ℃, reclaim extracting solution, and carry out reflux, extract, again 2 times, merge 3 extracting solution and be total to 5.3L by the 1st reflux, extract, operating condition in temperature;
(2) after filtration, concentrate with Rotary Evaporators, 100 ℃ of evaporation dryings obtain being Rhizoma Curcumae Longae water extract 22g that quality is 11% of a Rhizoma Curcumae Longae quality with the 5.3L extracting solution.
3) get Rhizoma Paridis saponin extract powder 10g and Rhizoma Curcumae Longae water extract 15g, be mixed with the Chinese medicine composition mixed liquor that contains 25 grammes per square metre building saponin extracts and Rhizoma Curcumae Longae water extract in every premium on currency solution altogether with antitumaous effect.
Embodiment three:
1) Rhizoma Paridis saponin extracts
(1) will cross Rhizoma Paridis coarse powder 500g behind 4 mesh sieves, the coarse powder that sieves is soaked in the alcoholic solution 1 hour of 3 mass per liter concentration 70%, then 90 ℃ of following heating and refluxing extraction of temperature 2 hours, reclaim 2.8 liters of extracting solution, and carry out reflux, extract, again 2 times by the 1st reflux, extract, operating condition, after merging 8.4 liters of 3 extracting solution, in the evaporation and obtain concentrated solution down of 80 ℃ of thickening temperatures, volume is 0.5L.
(2) it is to be uniformly dispersed in 20% alcoholic solution that the 0.5L concentrated solution that step (1) is obtained adds the 3.5L mass concentration, and centrifugation gets supernatant 3.9L.
(3) be 800mL with the flow velocity of 1.6L per hour by column volume with the 3.9L supernatant, blade diameter length ratio is the big adsorbent resin chromatographic column of 1: 5 D101, discards effluent; At first with mass concentration 40% ethanol water with the flow velocity eluting of 0.8L per hour 3 hours, reject eluent; With mass concentration 80% ethanol water with the flow velocity eluting of 1.6L per hour 5 hours, collect ethanol elution then, can obtain containing the Rhizoma Paridis saponin extract 15g of steroidal saponins mass fraction 37% behind the concentrating under reduced pressure vacuum drying.
2) preparation Rhizoma Curcumae Longae water extract
(1) Rhizoma Curcumae Longae 200g is ground into the 1cm fritter, adds deionized water 2L, extracted 1 hour for 100 ℃, reclaim extracting solution, and carry out reflux, extract, again 2 times, merge 3 extracting solution and be total to 5.8L by the 1st reflux, extract, operating condition in temperature;
(2) after filtration, concentrate with Rotary Evaporators, 100 ℃ of evaporation dryings obtain being Rhizoma Curcumae Longae water extract 23g that quality is 11.5% of a Rhizoma Curcumae Longae quality with the 5.8L extracting solution.
3) get Rhizoma Paridis saponin extract powder 10g and Rhizoma Curcumae Longae water extract 15g, be mixed with the Chinese medicine composition mixed liquor that contains 25 grammes per square metre building saponin extracts and Rhizoma Curcumae Longae water extract in every premium on currency solution altogether with antitumaous effect.
Embodiment four:
1) Rhizoma Paridis saponin extracts
(1) will cross Rhizoma Paridis coarse powder 500g behind 4 mesh sieves, the coarse powder that sieves is soaked in the alcoholic solution 1 hour of 4 mass per liter concentration 80%, then 90 ℃ of following heating and refluxing extraction of temperature 2 hours, reclaim 3.8 liters of extracting solution, and carry out reflux, extract, again 2 times by the 1st reflux, extract, operating condition, after merging 8.4 liters of 3 extracting solution, in the evaporation and obtain concentrated solution down of 80 ℃ of thickening temperatures, volume is 0.5L.
(2) it is to be uniformly dispersed in 30% alcoholic solution that the 0.5L concentrated solution that step (1) is obtained adds the 2.5L mass concentration, and centrifugation gets supernatant 2.4L.
(3) be 800mL with the flow velocity of 0.8L per hour by column volume with the 2.4L supernatant, blade diameter length ratio is the big adsorbent resin chromatographic column of 1: 5 D101, discards effluent; At first with mass concentration 40% ethanol water with the flow velocity eluting of 0.8L per hour 2 hours, reject eluent; With mass concentration 85% ethanol water with the flow velocity eluting of 1.6L per hour 5 hours, collect ethanol elution then, can obtain containing the Rhizoma Paridis saponin extract 15g of steroidal saponins mass fraction 37% behind the concentrating under reduced pressure vacuum drying.
2) preparation Rhizoma Curcumae Longae water extract
(1) Rhizoma Curcumae Longae 200g is ground into the 1cm fritter, adds deionized water 2L, extracted 1 hour for 100 ℃, reclaim extracting solution, and carry out reflux, extract, again 2 times, merge 3 extracting solution and be total to 5.8L by the 1st reflux, extract, operating condition in temperature;
(2) after filtration, concentrate with Rotary Evaporators, 100 ℃ of evaporation dryings obtain being Rhizoma Curcumae Longae water extract 24g that quality is 12% of a Rhizoma Curcumae Longae quality with the 5.8L extracting solution.
3) get Rhizoma Paridis saponin extract powder 10g and Rhizoma Curcumae Longae water extract 15g, be mixed with the Chinese medicine composition mixed liquor that contains 25 grammes per square metre building saponin extracts and Rhizoma Curcumae Longae water extract in every premium on currency solution altogether with antitumaous effect.
The test of pesticide effectiveness of Chinese medicine composition of the present invention is as follows:
Compositions so that embodiment obtains shows by the test to tumor-bearing mice in the body, uses separately with Rhizoma Paridis saponin and compares, and tumour inhibiting rate obviously improves, and does not have obvious toxic-side effects, and this Chinese medicine composition preparation method is simple, and active height and effect are obviously.
Above-mentioned Chinese medicine composition is carried out anti-tumor activity test, and its method and result are as follows:
One. the anti-tumor activity test method
1. the anti-tumor in vivo activity is tested
With embodiment preparation pharmaceutical composition 140ml carry out H22 liver cancer model, S180 solid tumor models, the test of pesticide effectiveness of Emhorn solid tumor models respectively.
1.1. animal modeling
1.1.1.H22 liver cancer model is got the full tumor-bearing mice of ascites after the inoculation H22 hepatoma carcinoma cell, the sterilization abdominal part, extracting milky ascites in superclean bench is the tumor source, the normal saline washing, ascites 2mL, the ratio of ascites and normal saline is 1: 4, normal saline 8mL, be made into cell suspension, extract 0.2mL, be inoculated in 48 mice oxters respectively with asepsis injector.Whole operation is carried out under aseptic condition.
1.1.2.S180 solid tumor models is got the full tumor-bearing mice of ascites behind the inoculation S180 cell, the sterilization abdominal part, extracting milky ascites in superclean bench is the tumor source, the normal saline washing, ascites 2mL, the ratio of ascites and normal saline is 1: 4, normal saline 8mL, be made into cell suspension, extract 0.2mL, be inoculated in 48 mice oxters with asepsis injector.Whole operation is carried out under aseptic condition.
1.1.3. the Emhorn solid tumor models is got the full tumor-bearing mice of ascites behind the inoculation Emhorn cell, the sterilization abdominal part, extracting milky ascites in superclean bench is the tumor source, the normal saline washing, ascites 2mL, the ratio of ascites and normal saline is 1: 4, normal saline 8mL, be made into cell suspension, extract 0.2mL, be inoculated in 48 mice oxters with asepsis injector.Whole operation is carried out under aseptic condition.
1.2. animal grouping and administration are inoculated and begun administration, every day 1 time next day: (1) model group gavages normal saline, every 0.2mL; (2) Rhizoma Paridis saponin group: gavage the Rhizoma Paridis saponin extract 0.2ml for preparing among the embodiment by 100mg/kg; (3) Rhizoma Curcumae Longae group: gavage the Rhizoma Curcumae Longae water extract 0.2ml for preparing among the embodiment by 150mg/kg; (3) Rhizoma Paridis saponin and Rhizoma Curcumae Longae compositions group: gavage the mixture 0.2ml for preparing among the embodiment by 250mg/kg.Two weeks, finish back cervical region next day dislocation in administration and put to death mice, its tumor tissue is got in dissection, weigh, survey tumour inhibiting rate: tumour inhibiting rate (%)=(the average tumor of the average tumor weight/normal saline of 1-administration group group is heavy) * 100%, win lung, spleen, liver is liquid-solid fixed with paraformaldehyde, weigh liver and spleen weight.Liver refers to=(liver weight/body weight) * 1000 that spleen refers to=(spleen weight/body weight) * 1000.
Two, anti-tumor activity test result
Table 1 Rhizoma Paridis saponin extract and Rhizoma Curcumae Longae water extract Chinese medicine composition are to H22 tumor-bearing mice tumour inhibiting rate result of the test
Table 2 Rhizoma Paridis saponin extract and Rhizoma Curcumae Longae water extract Chinese medicine composition are to H22 tumor-bearing mice immune organ weight index relatively
Table 3 Rhizoma Paridis saponin extract and Rhizoma Curcumae Longae water extract Chinese medicine composition are to S180 tumor-bearing mice tumour inhibiting rate result of the test
Table 4 Rhizoma Paridis saponin extract and Rhizoma Curcumae Longae water extract Chinese medicine composition are to S180 tumor-bearing mice immune organ weight index relatively
Table 5 Rhizoma Paridis saponin extract and Rhizoma Curcumae Longae water extract compositions are to Emhorn tumor-bearing mice tumour inhibiting rate result of the test
Table 6 Rhizoma Paridis saponin extract and Rhizoma Curcumae Longae water extract Chinese medicine composition are to Emhorn tumor-bearing mice immune organ weight index relatively
Three, conclusion
Through evidence, it is simple to the invention provides the Chinese medicine composition preparation method with antitumaous effect, has higher anti-tumor activity, can obviously suppress the tumor of tumor-bearing mice.Tumour inhibiting rate behind the compatibility significantly improves, refer to refer to as can be seen by liver with spleen, compare with independent use of Rhizoma Paridis saponin extract, liver refers to be significantly improved, spleen refers to also increase a lot, illustrate that Rhizoma Curcumae Longae water extract and Rhizoma Paridis saponin extract compatibility have Attenuation to liver, can effect of gain be arranged to immune organ simultaneously, the effect of enhance immunity is arranged.
Claims (2)
1. Chinese medicine composition with antitumaous effect, it is characterized in that, this Chinese medicine composition with antitumaous effect by Rhizoma Paridis saponin extract and Rhizoma Curcumae Longae water extract by mass ratio 2: 3, and to contain 25 milligrams of Rhizoma Paridis saponin extracts altogether in every ml water solution and the Rhizoma Curcumae Longae water extract is formed by compatibility, the quality that wherein contains the steroidal saponins in the Rhizoma Paridis saponin extract is 34～39%, all the other are unidentified saponins still, Rhizoma Curcumae Longae water extract main component is a curcumin, demethoxycurcumin, bisdemethoxycurcumin.
2. one kind by the described preparation method with Chinese medicine composition of antitumaous effect of claim 1, it is characterized in that comprising following process:
1) Rhizoma Paridis saponin extracts
(1) the Rhizoma Paridis coarse powder is crossed the 4-6 mesh sieve after, in Rhizoma Paridis powder and the ethanol water 1g of sieving: 4～8ml ratio, the Rhizoma Paridis powder was soaked in the alcoholic solution that mass concentration is 60-80% 1 hour, then 90 ℃ of following heating and refluxing extraction of temperature 2 hours, reclaim extracting solution, and carry out reflux, extract, again 2 times by the 1st reflux, extract, operating condition, merge 3 times extracting solution, extracting solution evaporates down for 80 ℃ in temperature and obtains concentrated solution;
(2) step (1) concentrated solution being added the volume multiple is that 5～10 times mass concentration is to be uniformly dispersed in 1～30% alcoholic solution, and supernatant is collected in centrifugation;
(3) supernatant that step (2) is obtained by the big adsorbent resin chromatographic column of D101, discards effluent with the flow velocity of 1～3 column volume per hour, and the adsorbent resin in the coupled columns carries out eluting then; At first with mass concentration 30%～40% ethanol water with the flow velocity eluting of 1～2 column volume per hour 2～4 hours, reject eluent; And then with mass concentration 70%～85% ethanol water and 1～2 column volume eluting 3～5 hours per hour, collect ethanol elution, to the ethanol elution concentrating under reduced pressure, obtain containing the Rhizoma Paridis saponin extract of the steroidal saponins of mass fraction 34～39% behind the vacuum drying;
2) preparation of Rhizoma Curcumae Longae water extract
(1) curcuma powder is broken into the 1cm fritter, in Rhizoma Curcumae Longae fritter and deionized water 1g: 8～10ml ratio adds Rhizoma Curcumae Longae in the deionized water, extracts half an hour for 100 ℃ in temperature, reclaims extracting solution, and carry out reflux, extract, again 2 times by the 1st reflux, extract, operating condition, merge 3 times extracting solution;
(2) extracting solution that step 1) is merged after filtration, filtrate concentrates with rotary evaporator, concentrated solution obtains being the Rhizoma Curcumae Longae water extract in 100 ℃ of evaporation dryings of temperature, quality is the 10-12% of Rhizoma Curcumae Longae quality;
3) Rhizoma Paridis saponin extract and Rhizoma Curcumae Longae water extract combination
Press the mass ratio 2: 3 of Rhizoma Paridis saponin extract powder and Rhizoma Curcumae Longae water extract, add in the deionized water, obtain having the Chinese medicine composition of antitumaous effect through ultrasonic mix homogeneously, to contain 25 milligrams of Rhizoma Paridis saponin extracts and Rhizoma Curcumae Longae water extract in every ml water solution altogether.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103142935A (en) * | 2013-03-18 | 2013-06-12 | 天津大学 | Traditional Chinese medicinal composition for treating lung cancer and liver cancer |
| CN103142934A (en) * | 2013-03-18 | 2013-06-12 | 天津大学 | Traditional Chinese medicinal composition for treating lung cancer and liver cancer |
| CN103169845A (en) * | 2013-03-13 | 2013-06-26 | 天津大学 | Traditional Chinese medicine composition having anti-liver cancer and anti-lung cancer effects |
| CN103169844A (en) * | 2013-03-13 | 2013-06-26 | 天津大学 | Traditional Chinese medicine composition having anti-lung cancer and anti-liver cancer effects |
| CN103933472A (en) * | 2014-04-04 | 2014-07-23 | 天津大学 | Traditional Chinese medicine composition for resisting liver cancer and lung cancer |
| CN104623215A (en) * | 2015-01-30 | 2015-05-20 | 天津大学 | Anti-tumor medicine composition |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101152408A (en) * | 2007-09-24 | 2008-04-02 | 天津大学 | Pharmaceutical composition having function cancer of the lungs against |
| CN101214342A (en) * | 2008-01-17 | 2008-07-09 | 天津大学 | Paris total saponins extract with tumor metastasis resisting action and pharmaceutical formulation thereof |
| CN101693035A (en) * | 2009-10-15 | 2010-04-14 | 天津大学 | Medicinal preparation with inhibiting effect on tumor metastasis |
| CN101904968A (en) * | 2010-07-27 | 2010-12-08 | 天津大学 | Preparation method of Chinese paris rhizome yam type saponin and anti-tumor medicinal preparation thereof |
| CN101912514A (en) * | 2010-07-27 | 2010-12-15 | 天津大学 | Paris rhizome total saponin capsules with anti-tumor effect and preparation method thereof |
-
2011
- 2011-06-17 CN CN 201110162554 patent/CN102225180A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101152408A (en) * | 2007-09-24 | 2008-04-02 | 天津大学 | Pharmaceutical composition having function cancer of the lungs against |
| CN101214342A (en) * | 2008-01-17 | 2008-07-09 | 天津大学 | Paris total saponins extract with tumor metastasis resisting action and pharmaceutical formulation thereof |
| CN101693035A (en) * | 2009-10-15 | 2010-04-14 | 天津大学 | Medicinal preparation with inhibiting effect on tumor metastasis |
| CN101904968A (en) * | 2010-07-27 | 2010-12-08 | 天津大学 | Preparation method of Chinese paris rhizome yam type saponin and anti-tumor medicinal preparation thereof |
| CN101912514A (en) * | 2010-07-27 | 2010-12-15 | 天津大学 | Paris rhizome total saponin capsules with anti-tumor effect and preparation method thereof |
Non-Patent Citations (6)
| Title |
|---|
| 《中南林业科技大学学报》 20090615 李湘洲等 姜黄色素的生物活性和提取分离研究进展 190-194 1-2 第29卷, 第03期 * |
| 《中国中医药信息杂志》 20060915 闵存云 黄松柏治疗肿瘤临床经验总结 87-88 1-2 第13卷, 第09期 * |
| 《中国民族民间医药》 20091031 胡兵等 SCI杂志中医药抗肿瘤文献研究 232-235 1-2 第13卷, 第20期 * |
| 《中国药业》 20040625 罗红霞等 姜黄素的提取及其含量测定研究进展 74-75 1-2 第13卷, 第06期 * |
| 《河南科技学院学报(自然科学版)》 20081215 牛生洋等 姜黄素的提取及应用研究进展 58-61 1-2 第34卷, 第04期 * |
| 《解放军药学学报》 20010430 韩婷等 姜黄的化学成分及药理活性研究进展 第95页第1部分姜黄的化学成分项下，第2部分姜黄的药理活性项下 1-2 第17卷, 第02期 * |
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