CN102225073A - Injection for treating spring allergy and allergic asthma and preparation method thereof - Google Patents
Injection for treating spring allergy and allergic asthma and preparation method thereof Download PDFInfo
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- CN102225073A CN102225073A CN2011101504878A CN201110150487A CN102225073A CN 102225073 A CN102225073 A CN 102225073A CN 2011101504878 A CN2011101504878 A CN 2011101504878A CN 201110150487 A CN201110150487 A CN 201110150487A CN 102225073 A CN102225073 A CN 102225073A
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- mesenchymal stem
- allergic asthma
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Abstract
The invention provides an injection for treating spring allergy and allergic asthma. The injection is characterized in that the injection takes cells as the main components; each millilitre of filler for injection contains 10,000,000-50,000,000 cell components; the cell components are human mesenchymal stem cells collected after the mesenchymal stem cells extracted from human bone marrows are subjected to low-serum or serum-free culture in vitro and amplification; and the collected mesenchymal stem cells are used for preparing the injection which can be used as the clinical intravenous drip. The injection is used for treating spring allergy and allergic asthma. The invention provides a preparation method of the injection. The injection has the following advantages: the obvious clinical effect can be shown within 7 days after the injection is injected; and the injection has lasting effect and simple and convenient treatment procedures.
Description
Technical field
The present invention relates to a kind of injection for the treatment of irritated and allergic asthma in spring and preparation method thereof.
Background technology
Treat for a long time spring irritated with allergic asthma be exactly a difficult problem of medical circle, many at present employing hormone antiallergic and anti-inflammatory treatment, but curative effect is relatively poor, and symptom the lighter can play certain therapeutical effect, but can only symptomatic treatment, and poor effect for sending out the author repeatedly.Spring, allergy all belonged to autoimmune disease with allergic asthma, its pathogeny be spring irritated with allergic asthma be by the various kinds of cell chronic air flue and the scytitis that participate in of mastocyte, eosinophilic granulocyte and T lymphocyte particularly, the verified chronic anaphylaxis inflammation at skin and respiratory tract of immunopathology is similar, the participation of Th2 cell, hypertrophy/basophilic leukocyte, eosinophilic granulocyte and IgE is all arranged, inflammatory chemical mediators such as the cytokine of participation such as IL-4.At sensitive individual, with bonded tissue mast cell of IgE and basophilic granulocyte, behind the contact allergen, be activated then, discharge histamine, leukotriene and other media.This reaction the lighter can cause allergy in spring, and weight person can cause that speed sends out the nose irritation of property, as sneeze and rhinorrhea by neural mediation, and the nasal obstruction that causes by the congestion of blood vessel etc.Sending out symptom in the speed of lower respiratory tract is bronchospasm and mucous high secretion, causes cough, and dyspnea is uncomfortable in chest and pant.
Summary of the invention
In order to overcome the deficiency of irritated medicine with allergic asthma in above-mentioned treatment spring, the object of the present invention is to provide irritated injection with allergic asthma in a kind of instant effect, the persistent treatment of curative effect spring.
Another object of the present invention provides the preparation method of this injection.
For achieving the above object, the present invention is by the following technical solutions:
A kind of irritated injection with allergic asthma in spring for the treatment of, this agent mainly is made up of cell component, and the content of cell component is 1,000 ten thousand to 50,000,000 in every milliliter of injection agent; Described cell component is that the mescenchymal stem cell that extracts from people's bone marrow, is purified into is collected the mesenchymal stem cells MSCs that obtains after external low serum or serum-free culture and amplification.
Injection of the present invention can also contain some auxiliary elements except cell component, described auxiliary element can be this area common can be as the composition of cell lysing agent, as vitamin, glucose, etc. ooze normal saline or the like.
In addition, the present invention also provides the preparation method of a kind of described treatment allergy in spring with the injection of allergic asthma, and this method step is: described cell component is that the mescenchymal stem cell that extracts from people's bone marrow, is purified into is collected the mesenchymal stem cells MSCs that obtains after external low serum or serum-free culture and amplification.
The myeloid tissue that the present invention gathered is to extract to obtain from people's bone marrow.
In injection preparation method provided by the present invention,, after external low serum or serum-free culture and amplification, collect the mesenchymal stem cells MSCs that obtains again with deriving from mescenchymal stem cell extraction, the purification of people's bone marrow.
Get specimen and cell cultivation process and adopt method in common.
Injection preparation: will cultivate quantity and meet the requirements of cell and at first use trypsin to carry out digestion process, and make it break away from the culture dish bottom surface, and clean several at the DMEM that uses no extrinsic protein, the removal exogenous growth factor and active substance.The cell of collecting is directly joined in 5% the glucose injection that contains aminoacid, normal saline and vitamin, mixing is made into injection again, and total number of cell component is 1,000 ten thousand to 5,000 ten thousand in per 150 milliliters of injections.
Treatment step: injection is carried out intravenous drip, be totally lost in 40 minutes.
Compare with existing treatment means, injection of the present invention have treatment easy, have no side effect, instant effect, characteristics such as curative effect is lasting.
Description of drawings
Fig. 1 cultivates successful cell morphology photo under 100 times of optical microscopes;
Fig. 2 is the treatment allergy in spring of a specific embodiment of the present invention and the photo of injection in packaging bag of allergic asthma.
The specific embodiment
Further describe injection of treatment allergy in spring provided by the present invention and allelgic asthma disease and preparation method thereof below, but therefore the present invention is not subjected to any restriction.
Embodiment: a kind of preparation for the treatment of the injection of irritated and allergic asthma in spring
One. the extraction of bone marrow sample
Patient's marrow blood is extracted in routine operation sterilization back, adds centrifugal degrease layer behind the anticoagulant, add normal saline dilute back three times centrifugal, the cell that obtains behind the washed cell is cultivated.
Two. the preparation of cell suspension
Preparation injection: will cultivate quantity and meet the requirements of cell and at first use trypsin to carry out digestion process, and make it break away from the culture dish bottom surface, and re-use the DMEM cleaning 3 times of no extrinsic protein.The cell of collecting is joined 5% contain in the amino acid whose glucose injection, and mixing is made into injection, and total number of cell component is 1,000 ten thousand to 5,000 ten thousand in every milliliter of injection agent.
Virus detects: skin histology and the cell after 4 weeks of cultivation carry out the check of HIV and hepatitis virus, determine that this is virus-free cell.
The immunoreation test: the 4th week was carried out skin test (with penicillin skin test method), no immunoreation with the 0.1ml cell injection to the operation receiveing person.
Finished product detection:
1) outward appearance: injection is colourless suspension.
2) cell divide calibrating in this product:
Mesenchymal stem cells MSCs: adopt the morphocytology method to observe.
3) sterility test: undertaken by " Chinese biological goods rules " (2000 editions) general rule " biological product sterility test rules " A/B item, the result meets aseptic requirement.
Three. treatment
Be used for irritated and each 1 example of allergic asthma case in spring.
1. check before the treatment that every index is all normal, comprising: hematuria is routine, electrocardiogram, hepatic and renal function, HIV, HbsAg just.
2. sterilized with routine disinfection in the injection site.
3. carry out intravenous drip infusion.
Four, taboo:
The careful usefulness of anemia of pregnant woman.
Five, effect is described
The back three days sxs of infusing obviously alleviated after one week, two week the back symptoms disappear substantially.
Claims (3)
1. injection for the treatment of irritated and allergic asthma in spring, this injection mainly is grouped into by human marrow mesenchymal stem cell one-tenth, and the content of cell component is 1,000 ten thousand to 5,000 ten thousand in every milliliter of injection; Described cell component is that the mescenchymal stem cell that extracts from people's bone marrow, is purified into is collected the mesenchymal stem cells MSCs that obtains after external low serum or serum-free culture and amplification.
2. method for preparing the injection of irritated and allergic asthma in the described treatment of claim 1 spring may further comprise the steps:
(1) mesenchymal stem cells MSCs that adopts people's bone marrow to separate, be purified into for the source carries out external low serum or serum-free culture and amplification, collects resulting a large amount of mesenchymal stem cells MSCs;
(2) mesenchymal stem cells MSCs of collecting is prepared into the treatment allergy in spring or the injection of allergic asthma; The content of cell component is 1,000 ten thousand to 5,000 ten thousand in every milliliter of this injection.
3. the cell of collecting is directly joined in 5% the glucose injection that contains aminoacid, normal saline and vitamin, mixing is made into injection 150ml injection.Therapeutic process is: every bag injection 150ml, through intravenous drip, to inject once in per 40 days, and three injections are a course of treatment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101504878A CN102225073A (en) | 2011-06-07 | 2011-06-07 | Injection for treating spring allergy and allergic asthma and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101504878A CN102225073A (en) | 2011-06-07 | 2011-06-07 | Injection for treating spring allergy and allergic asthma and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102225073A true CN102225073A (en) | 2011-10-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011101504878A Pending CN102225073A (en) | 2011-06-07 | 2011-06-07 | Injection for treating spring allergy and allergic asthma and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109331187A (en) * | 2018-12-10 | 2019-02-15 | 天津长和生物技术有限公司 | The evaluation method and application of mescenchymal stem cell preparation sensitization |
-
2011
- 2011-06-07 CN CN2011101504878A patent/CN102225073A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109331187A (en) * | 2018-12-10 | 2019-02-15 | 天津长和生物技术有限公司 | The evaluation method and application of mescenchymal stem cell preparation sensitization |
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
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Application publication date: 20111026 |