CN102206265A - Process of producing cytochrome c solution - Google Patents
Process of producing cytochrome c solution Download PDFInfo
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- CN102206265A CN102206265A CN2011100947559A CN201110094755A CN102206265A CN 102206265 A CN102206265 A CN 102206265A CN 2011100947559 A CN2011100947559 A CN 2011100947559A CN 201110094755 A CN201110094755 A CN 201110094755A CN 102206265 A CN102206265 A CN 102206265A
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- cytochrome
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- crude product
- mammals
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- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses a process of producing a cytochrome c solution which is extracted from hearts of mammals. The method comprises the steps of extracting the hearts of the mammals with acid water, adsorbing by using an absorbing material, eluting the acid water, salting out, settling and dialyzing to prepare a crude product of the cytochrome c solution, and finally carrying out ion exchanging and purifying on the crude product to obtain the refined cytochrome c solution.
Description
Technical field
The invention belongs to chemical technology field, especially relate to a kind of production technique of cytochrome c solution.
Background technology
Cytochrome c also claims thin by the third, be a kind of be the respiratory enzyme of prothetic group with the iron porphyrin, in biological oxidation process, it is a very important electron transit mediator, its action principle is under the situation that enzyme exists, to oxidation, also original enzymatic action rapidly of tissue.Common exogenous cytochrome c can not enter healthy cell, but when anoxic, the permeability of cytolemma increases, and cytochrome c might enter in cell and the plastosome, strengthens cellular oxidation, improves the utilization of oxygen.
Cytochrome c is the cellular respiration agonist, and as the first aid and the adjuvant drug of histanoxia treatment, as carbon monoxide poisoning, poisoning with hypnotic also is used for cerebrovascular disorder, the treatment of apoplexy sequela.
Summary of the invention
It is simple to the purpose of this invention is to provide a kind of technology, the production method of the cytochrome c solution that yield is higher.
Advantage of the present invention: the cytochrome c solution process of producing with this method is simple, and stable, with low cost, yield reaches more than 85%.The present invention has adopted sorbing material A at acid water extracting liquid, and pair cell pigment c solution crude product, through ion exchange resin treatment, gets fine cytochrome c solution elaboration, can be used for clinical.Technical scheme of the present invention is: a kind of production method of cytochrome c solution, and its technological process is as follows:
A) the qualified Mammals of veterinary inspector of learning from else's experience;
B) mammiferous cardiac muscle is through acid extraction;
C) acid water extracting liquid is used the inorganic salt solution wash-out after sorbing material A absorption;
D) elutriant is adjusted to than the 1.22-1.44 that weighs with inorganic salt;
E) elutriant trichloroacetic acid precipitation, filter throw out;
F) throw out is used the dialysis method purifying after with water dissolution, through after the assay was approved, cytochrome c solution crude product;
G) cytochrome c solution crude product after ion exchange resin absorption, is used the inorganic salt solution wash-out;
H) elutriant dialysis method purifying through after the assay was approved, gets cytochrome c solution elaboration, with preservation below-5 ℃.
Embodiment
Further describe the present invention with embodiment below, help the present invention and advantage thereof, the understanding of better effects if, but described embodiment only is used to illustrate the present invention rather than restriction the present invention.
Embodiment
A) the qualified Mammals of veterinary inspector of learning from else's experience.
B) mammiferous myocardium 10Kg adds the 15Kg purified water, transfers pH4.0, mechanical stirring 3 hours with 2mol/L sulfuric acid.After leaving standstill 0.5 hour, centrifuging gets the about 20Kg of acid water extracting liquid.
C) acid water extracting liquid is transferred pH8.0 with 2mol/L ammoniacal liquor, adds the adsorbents adsorb resin by 4% amount (w/v), divides three times whip attachment.Rinse the polymeric adsorbent that has adsorbed well the back upper prop with purified water,, get elutriant with 26% ammonium sulfate (d1.15) wash-out.
D) elutriant is adjusted to than the 1.22-1.44 that weighs with ammonium sulfate;
E) elutriant adds 20% trichoroacetic acid(TCA) 25ml according to every 1000ml and produces precipitation, filter throw out.
F) throw out carries out the dialysis method purifying with dialysis tubing after with water dissolution, through after the assay was approved, cytochrome c solution crude product.
G) cytochrome c solution crude product is after ion exchange resin M 800 absorption, with 0.59mol/LNaCl and 90mmol/L Na
2HPO
4Eluant solution.
H) the elutriant dialysis tubing of packing into is used the dialysis method purifying, through after the assay was approved, cytochrome c solution elaboration, in preserving below-5 ℃.
Claims (3)
1. the production technique of a cytochrome c solution is characterized in that producing cytochrome c solution with the Mammals heart.
2. according to the production method of claims 1 described a kind of cytochrome c, it is characterized in that described Mammals is a kind of of pig, dog, ox, sheep, Malaysia and China, is preferably pig, ox.
3. according to the production technique of claims 1 or 2 described cytochrome c solution, it is characterized in that: get mammalian heart through acid extraction, sorbing material A absorption, the sour water wash-out is saltoutd, and precipitation and dialysis method are produced cytochrome c solution crude product.Crude product finally obtains refining cytochrome c solution through ion-exchange purification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100947559A CN102206265A (en) | 2011-04-15 | 2011-04-15 | Process of producing cytochrome c solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100947559A CN102206265A (en) | 2011-04-15 | 2011-04-15 | Process of producing cytochrome c solution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102206265A true CN102206265A (en) | 2011-10-05 |
Family
ID=44695355
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011100947559A Pending CN102206265A (en) | 2011-04-15 | 2011-04-15 | Process of producing cytochrome c solution |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102206265A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109694408A (en) * | 2017-10-20 | 2019-04-30 | 中蓝晨光化工研究设计院有限公司 | A kind of ion-exchange purification method of cromoci |
| CN114920828A (en) * | 2022-06-17 | 2022-08-19 | 德博尔(成都)生物科技有限公司 | Method for preparing cytochrome C |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1106019A (en) * | 1994-08-04 | 1995-08-02 | 陈庆功 | Production technology of crude cytochrome C |
| CN101709086A (en) * | 2009-10-26 | 2010-05-19 | 烟台东诚生化股份有限公司 | New method for preparing cytochrome C |
-
2011
- 2011-04-15 CN CN2011100947559A patent/CN102206265A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1106019A (en) * | 1994-08-04 | 1995-08-02 | 陈庆功 | Production technology of crude cytochrome C |
| CN101709086A (en) * | 2009-10-26 | 2010-05-19 | 烟台东诚生化股份有限公司 | New method for preparing cytochrome C |
Non-Patent Citations (4)
| Title |
|---|
| 《家畜生态学报》 20070531 马志科 "秦川牛心肌细胞色素C 提取纯化及鉴定的研究" 第90-93页 第28卷, 第3期 * |
| 《应用化学》 19970430 史林启,何炳林 "微多相聚氨酯大孔树脂吸附细胞色素c 的研究" 第113-115页 摘要，结果与讨论，图1-2，表1，3 3 第14卷, 第113期 * |
| 史林启,何炳林: ""微多相聚氨酯大孔树脂吸附细胞色素c 的研究"", 《应用化学》, vol. 14, no. 113, 30 April 1997 (1997-04-30) * |
| 马志科: ""秦川牛心肌细胞色素C 提取纯化及鉴定的研究"", 《家畜生态学报》, vol. 28, no. 3, 31 May 2007 (2007-05-31), pages 90 - 93 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109694408A (en) * | 2017-10-20 | 2019-04-30 | 中蓝晨光化工研究设计院有限公司 | A kind of ion-exchange purification method of cromoci |
| CN114920828A (en) * | 2022-06-17 | 2022-08-19 | 德博尔(成都)生物科技有限公司 | Method for preparing cytochrome C |
| CN114920828B (en) * | 2022-06-17 | 2024-02-02 | 德博尔(成都)生物科技有限公司 | Method for preparing cytochrome C |
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Application publication date: 20111005 |