CN102204439A - Alfalfa seed pretreatment method for improving vigor of seedlings and saline-alkali resistance - Google Patents
Alfalfa seed pretreatment method for improving vigor of seedlings and saline-alkali resistance Download PDFInfo
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Abstract
The invention discloses an alfalfa seed pretreatment method for improving vigor of seedlings and saline-alkali resistance. The alfalfa seed pretreatment method comprises the steps of: firstly putting seeds in an air-permeable mesh bag, placing in a dry container filled with silica gel and sealing with aluminized paper, drying the seeds with the silica gel at the room temperature to reduce the water content of the seeds to be 4.59 to 7.09 percent, sealing the dried seeds with the aluminized paper, placing in a dry container filled with drying silica gel, and storing the seeds at the room temperature. Before seeding, the highly dried and stored seeds are sequentially placed in closed environments the relative humidity of which is increased in sequence, and the water content of seeds is improved gradually. The seeds are highly dried so that the vigor of seeds and the stress resistance of seedlings can be improved, thus the method is a simple, energy-saving and effective seed pretreatment method which can prolong the storage life of seeds. Simultaneously, the osmotic stress resistance of seeds in the germination period is enhanced, and the osmotic stress resisting function of seedlings in a saline environment is improved. The water saving effect and the drought tolerance are achieved in alfalfa cultivation by adopting a method of improving the seedling regularity and germinating speed.
Description
Technical field
The present invention relates to a kind of pretreating method for seed, relate in particular to the alfalfa seed preprocess method that improves seedling vigor and saline and alkaline resistance.
Background technology
Alfalfa seed preliminary treatment measure at present commonly used mainly contains that kind of skin scratches, the initiation measure of seed pelleting, chemical agent seed soaking seed dressing and various seeds.Adopt physical method to break in the seed coat drawing, also very easily introduce pathogen when increasing emergence rate, the maintenance of seed vitality is caused certain injury; The seed dressing operation is numerous and diverse; Seed pelleting cost costliness; Seed commonly used at present causes reagent and mainly comprises polyethylene glycol, organic macromolecule and potassium nitrate such as cross-linking type PAA and polyvinyl alcohol, the little molecule of mineral salt such as sodium chloride and U-Ramin MC, chemical agent mainly contains gibberellic acid, clorox etc., yet, because the characteristics of different germplasms differ, selected processing drug concentration is also different, caused certain inconvenience for the seed soaking seed dressing and the initiation processing of different germplasms, and drug concentration is on the low side when handling is not enough to corrode the hard and thick kind skin of seed, promote seed germination, be not enough to kill the germ that seed carries and reduce the seedling death rate, the chemicals treatment excessive concentration then can produce adverse influence to embryo and interior enzymic activity, and then the normal growth of inhibition seedling, increase certain degree of difficulty to processing, simultaneously, part reagent cost costliness, the use in enormous quantities in also being unfavorable for producing.
Summary of the invention
Technical problem to be solved by this invention is to overcome the deficiencies in the prior art, and a kind of effective raising seedling vigor of germ and pretreating method for seed of saline and alkaline resistance introduced easy to control, difficult is provided.
Technical solution problem of the present invention adopts following technical scheme:
A kind of alfalfa seed preprocess method that improves seedling vigor and saline and alkaline resistance comprises the processing of super dry treatment of seed and rehydration;
Described super dry treatment is: seed is inserted in the gas permeability mesh bag, evenly be positioned in the drier that fills with silica gel, described silica gel for through 120 ℃ of hyperthermia dryings to constant weight and be cooled to the oven dry silica gel of room temperature, seed is 1:10 with the mass ratio of oven dry silica gel, and change a silica gel every 23～25h, under sealing state, use oven dry silica gel with the at room temperature dry 12～216h of seed, making seed moisture in mass percent is 4.59～7.09%, dried seed seals with aluminium-foil paper, place the drier that fills oven dry silica gel, room temperature storage 6～365 days;
Described rehydration is treated to: will surpass the closed environment that the seed after dry treatment is also preserved places relative moisture to raise successively successively before the sowing, and improve seed moisture content gradually.
Further, in the super dry treatment process, preferably changed a silica gel every 24 hours.
Further, the environment of rehydration processing is followed successively by: CaCl
2Closed environment, the NH of the relative temperature 35.0% that saturated solution is regulated
4The closed environment of the relative moisture 100% that the closed environment of the relative moisture 70% that the Cl saturated solution is regulated and distilled water are regulated, multiple time for water treatment is 24～26 hours in each closed environment, and each saturated solution all is the saturated solutions under 20 ℃ of the temperature.
Further, the super dry seeds after the storage was answered time for water treatment preferred 24 hours in each closed environment.
Further, during super dry treatment, the preferred 1:10 of mass ratio of described seed and oven dry silica gel.
Further, described replacing silica gel is spaced apart 24h once, and described oven dry silica gel is for drying to constant weight and cooled silica gel through 120 ℃.
Further, be 72～120h described drying time, and making seed moisture in mass percent is that 5.18～5.72% back seed seedling vigor and saline and alkaline resistances of preserving are best.
Further, described seed is an alfalfa seed.
Beneficial effect of the present invention is embodied in:
The alfalfa seed preprocess method of raising seedling vigor of the present invention and saline and alkaline resistance; the water content of seed is reduced to the safe water content of quality saving; promptly at ambient temperature alfalfa seed is surpassed dry treatment; reduce the generation of lipid peroxidation by the activity of integrality and cell structure, the increase seed internal protection material that keeps film, the stability of keeping the radicals scavenging system and enhancing enzyme system; improving the resistance of seed vitality and gained seedling, is simple, energy-conservation, the effective seed preconditioning technique that can prolong the seed storage life-span.And this method adopts the quick super dried mode of air drying agent that seed moisture content is reduced, to improve the content of seed vigor and impermeabilisation material, the resistance to osmotic stress strengthens in the budding period to make seed, realizes improving the seedling effect that impermeabilisation is coerced in saline-alkali environment.And by improving speed of emergence, the mode that improves the regularity of emerging realizes the water savingization and the drought resistance of clover cultivation.Pretreating method for seed of the present invention in addition scratches raising seed seedling vigor with kind of a skin and saline and alkaline resistance is compared, and is difficult for introducing germ, compares easy to control with the preprocess method of seed pelleting, chemical agent seed soaking seed dressing.
Description of drawings
Fig. 1 is the alfalfa seed seedling plant height comparison diagram under neutral environment after embodiment 1～8 disposal methods;
Fig. 2 is the alfalfa seed seedling plant height comparison diagram under salt stress after embodiment 1～8 disposal methods;
Fig. 3 is the alfalfa seed seedling plant height comparison diagram under alkaline stress after embodiment 1～8 disposal methods;
Fig. 4 is the alfalfa seed seedling leaves number comparison diagram under neutral environment after embodiment 1～8 disposal methods;
Fig. 5 is the alfalfa seed seedling leaves number comparison diagram under salt stress after embodiment 1～8 disposal methods;
Fig. 6 is the alfalfa seed seedling leaves number comparison diagram under alkaline stress after embodiment 1～8 disposal methods;
Fig. 7 is the alfalfa seed seedling leaf area comparison diagram under neutral environment after embodiment 1～8 disposal methods;
Fig. 8 is the alfalfa seed seedling leaf area comparison diagram under salt stress after embodiment 1～8 disposal methods;
Fig. 9 is the alfalfa seed seedling leaf area comparison diagram under alkaline stress after embodiment 1～8 disposal methods;
Figure 10 is the alfalfa seed seedling chlorophyll content comparison diagram under neutral environment after embodiment 1～8 disposal methods;
Figure 11 is the alfalfa seed seedling chlorophyll content comparison diagram under salt stress after embodiment 1～8 disposal methods;
Figure 12 is the alfalfa seed seedling chlorophyll content comparison diagram under alkaline stress after embodiment 1～8 disposal methods.
Embodiment
The invention will be further described below in conjunction with embodiment, east, used Gansu Province alfalfa seed, and cleanliness 98.5%, initial germination rate 78.84% is in mass fraction original water content 9.05%.Before on-test, keep in Dark Place in seed ventilation under room temperature, and before the beginning preliminary treatment, the seed storage time is 6 months.
Alfalfa seed 5.0g inserts in the nylon net bag of good air permeability with east, Gansu Province, evenly be positioned in the drying basin that fills with silica gel, the ratio of seed and silica gel is the 1:10(mass ratio), 23h changes a silica gel afterwards, and silica gel also cools off through 120 ℃ of hyperthermia dryings to constant weight; In airtight container with silica gel with the at room temperature dry 12h of seed, seed moisture content is 7.09% (mass fraction), dried seed places the drying basin that fills oven dry silica gel with the sealing of double-layer aluminum-foil paper, places room temperature condition under and preserves 6 days.
To surpass the closed environment that the seed after dry treatment is also preserved places relative moisture to raise successively successively before the sowing, improve seed moisture content gradually.The environment that the rehydration of using in the present embodiment is handled is followed successively by: CaCl
2Closed environment, the NH of the relative moisture 35.0% that saturated solution is regulated
4The closed environment of the relative moisture 100% that the closed environment of the relative moisture 70% that the Cl saturated solution is regulated and distilled water are regulated, multiple time for water treatment is 24 hours in each closed environment, improves seed moisture content gradually.
Alfalfa seed 5.0g inserts in the nylon net bag of good air permeability with east, Gansu Province, evenly be positioned in the drying basin that fills with silica gel, the ratio of seed and silica gel is the 1:10(mass ratio), 24h changes a silica gel afterwards, and silica gel also cools off through 120 ℃ of hyperthermia dryings to constant weight; In airtight container with silica gel with the at room temperature dry 24h of seed, seed moisture content is 6.93% (mass fraction), dried seed places the drying basin that fills oven dry silica gel with the sealing of double-layer aluminum-foil paper, places room temperature condition under and preserves 30 days.
To surpass the closed environment that the seed after dry treatment is also preserved places relative moisture to raise successively successively before the sowing, improve seed moisture content gradually.The environment that the rehydration of using in the present embodiment is handled is followed successively by: CaCl
2Closed environment, the NH of the relative moisture 35.0% that saturated solution is regulated
4The closed environment of the relative moisture 100% that the closed environment of the relative moisture 70% that the Cl saturated solution is regulated and distilled water are regulated, multiple time for water treatment is 25 hours in each closed environment, improves seed moisture content gradually.
East alfalfa seed 5.0g inserts in the nylon net bag of good air permeability with pale reddish brown Gansu Province, evenly be positioned in the drying basin that fills with silica gel, the ratio of seed and silica gel is the 1:10(mass ratio), 25h changes a silica gel afterwards, and silica gel also cools off through 120 ℃ of hyperthermia dryings to constant weight; In airtight container with silica gel with the at room temperature dry 48h of seed, seed moisture content is 6.36% (mass fraction), dried seed places the drying basin that fills oven dry silica gel with the sealing of double-layer aluminum-foil paper, places room temperature condition under and preserves 60 days.
To surpass the closed environment that the seed after dry treatment is also preserved places relative moisture to raise successively successively before the sowing, improve seed moisture content gradually.The environment that the rehydration of using in the present embodiment is handled is followed successively by: CaCl
2Closed environment, the NH of the relative moisture 35.0% that saturated solution is regulated
4The closed environment of the relative moisture 100% that the closed environment of the relative moisture 70% that the Cl saturated solution is regulated and distilled water are regulated, multiple time for water treatment is 26 hours in each closed environment, improves seed moisture content gradually.
Alfalfa seed 5.0g inserts in the nylon net bag of good air permeability with east, Gansu Province, evenly be positioned in the drying basin that fills with silica gel, the ratio of seed and silica gel is the 1:10(mass ratio), 24h changes a silica gel afterwards, and silica gel also cools off through 120 ℃ of hyperthermia dryings to constant weight; In airtight container with silica gel with the at room temperature dry 72h of seed, seed moisture content is 5.72% (mass fraction), dried seed places the drying basin that fills oven dry silica gel with the sealing of double-layer aluminum-foil paper, places room temperature condition under and preserves 120 days.
The rehydration processing method is identical with processing method among the embodiment 1.
Alfalfa seed 5.0g inserts in the nylon net bag of good air permeability with east, Gansu Province, evenly be positioned in the drying basin that fills with silica gel, the ratio of seed and silica gel is the 1:10(mass ratio), 24h changes a silica gel afterwards, and silica gel also cools off through 120 ℃ of hyperthermia dryings to constant weight; In airtight container with silica gel with the at room temperature dry 96h of seed, seed moisture content is 5.46% (mass fraction), dried seed places the drying basin that fills oven dry silica gel with the sealing of double-layer aluminum-foil paper, places room temperature condition under and preserves 180 days.
The rehydration processing method is identical with processing method among the embodiment 1.
Alfalfa seed 5.0g inserts in the nylon net bag of good air permeability with east, Gansu Province, evenly be positioned in the drying basin that fills with silica gel, the ratio of seed and silica gel is the 1:10(mass ratio), 24h changes a silica gel afterwards, and silica gel also cools off through 120 ℃ of hyperthermia dryings to constant weight; In airtight container with silica gel with the at room temperature dry 120h of seed, seed moisture content is 5.18% (mass fraction), dried seed places the drying basin that fills oven dry silica gel with the sealing of double-layer aluminum-foil paper, places room temperature condition under and preserves 220 days.
The rehydration processing method is identical with processing method among the embodiment 1.
Embodiment 7
Alfalfa seed 5.0g inserts in the nylon net bag of good air permeability with east, Gansu Province, evenly be positioned in the drying basin that fills with silica gel, the ratio of seed and silica gel is the 1:10(mass ratio), 24h changes a silica gel afterwards, and silica gel also cools off through 120 ℃ of hyperthermia dryings to constant weight; In airtight container with silica gel with the at room temperature dry 144h of seed, seed moisture content is 4.97% (mass fraction), dried seed places the drying basin that fills oven dry silica gel with the sealing of double-layer aluminum-foil paper, places room temperature condition under and preserves 300 days.
The rehydration processing method is identical with processing method among the embodiment 1.
Alfalfa seed 5.0g inserts in the nylon net bag of good air permeability with east, Gansu Province, evenly be positioned in the drying basin that fills with silica gel, the ratio of seed and silica gel is the 1:10(mass ratio), 24h changes a silica gel afterwards, and silica gel also cools off through 120 ℃ of hyperthermia dryings to constant weight; In airtight container with silica gel with the at room temperature dry 216h of seed, seed moisture content is 4.59% (mass fraction), dried seed places the drying basin that fills oven dry silica gel with the sealing of double-layer aluminum-foil paper, places room temperature condition under and preserves 365 days.
The rehydration processing method is identical with processing method among the embodiment 1.
Comparative trial
Be better explanation advantage of the present invention, below the seed sample that will obtain according to method described in the embodiment 1 to 8 with without super dried, east, Gansu Province of water content 9.03% (mass fraction) alfalfa seed is done to contrast and is tested.
A, salt alkaline stress method for designing:
With NaCl and Na
2SO
4Press the 9:1 mixed in molar ratio, according to the salt tolerant degree of east, Gansu Province alfalfa, the suitableeest salinity of selecting 40mmol/L by the preliminary experiment in the culture dish is that pot experiment is coerced concentration, to simulate typical salt stress environment.
With NaHCO
3And Na
2CO
3Press the 9:1 mixed in molar ratio, simulate typical alkaline stress environment, select the suitableeest alkaline stress concentration of 15mmol/L, as the alkaline stress concentration of pot experiment.
Preparation Hoagland nutrient solution, macroelement: Ca(NO
3)
2417mg/L; KNO
3607mg/L; MgSO
4493mg/L; (NH
4)
3PO
4115mg/L; Trace element: H
3BO
32.86 mg/L; MnCl
24H
2O 1.81 mg/L; ZnSO
47H
2O 0.22 mg/L; CuSO
45H
2O 0.08 mg/L; H
2MoO
4H2O 0.02 mg/L.
Salt stress nutrient solution NaOH(1mol/L) or HCl(1mol/L) regulate pH value to 7.0.
Selected the cleaning river sand of 1mm sieve for use, with 4mol/L salt acidleach 5h, with distilled water with river sand embathe repeatedly to pH value be 7.0 ± 0.3, dry behind the deionized water rinsing 2～4 times.
B, sowing
Select 25 of each super dry treatment alfalfa seeds at random, pack in the plastic flowerpot that fills 480g cleaning river sand of diameter 7cm, high 7.5cm, volume 200 mL, cover behind the husky 40g each is handled basin insert respectively complete nutrition liquid and and fill in the tank of corresponding complete Hoagland nutrient solution of coercing concentration, make culture fluid at the bottom of basin, upwards slowly infiltrate into the sandy soil surface.
Emerge 22 days the time, measure each and handle form and the physiological and biochemical index of seedling down.
C, result of the test
(a) super dry treatment to the influence of seedling plant height under neutral environment and the salt alkaline stress referring to Fig. 1 to 3.
Referring to Fig. 1, under the neutral environment, during seed sprouting 5d, it is not remarkable that each handles differences; Emerge 10 days the time, the super dry treatment plant height of 5.72% water content exceeds contrast 16.82%; When emerging 15d, the super dry treatment plant height of 5.72% water content exceeds contrast 19.87%; When emerging 22d, in mass fraction water content 6.36～5.18% respectively handle plant height exceed the contrast 2.21～18.38%, difference has statistical significance (P＜0.05).
Referring to Fig. 2, under the salt stress, during seed sprouting 5d, 4.97% water content is handled the seedling plant height and is exceeded contrast 43.59%; Emerge 10 days the time, the super dry treatment plant height of 5.72～4.97% water content exceeds contrast 21.59～46.59%; When emerging 15d, the super dry treatment plant height of 5.46～4.97% water content exceeds contrast 8.16～23.81%; When emerging 22d, all super dry treatment seedling plant heights all exceed contrast, are 8.47～59.32% of contrast, and significant difference is (P＜0.05) significantly.
Referring to Fig. 3, under the alkaline stress, during seed sprouting 5d, handle the seedling plant height in mass fraction water content 5.36% and exceed contrast 31.91%; Emerge 10 days the time, exceed contrast 2.08～44.70% in the super dry treatment plant height of mass fraction water content 5.72～5.18%; When emerging 15d, all super dry treatment plant heights all exceed contrast, are 5.2～37.01% of contrast; When emerging 22d, all super dry treatment seedling plant heights equally all exceed contrast, are 3.41～47.33% of contrast, and significant difference is (P＜0.05) significantly.
(b) super dry treatment to the influence of seedling leaves number under neutral environment and the salt alkaline stress referring to Fig. 4 to 6.
Referring to Fig. 4, under the neutral environment, 6.36,5.46 and contrast seedling in the seedling leaves number that the mass fraction water content 4.59% surpasses dry treatment and exceed 3.95,15.79 and 2.63% respectively, gap has statistical significance (P＜0.05).
Referring to Fig. 5, under the salt stress, all super dry treatment seedling leaves number averages are higher than control treatment, are 112.5～150% of contrast, and gap has statistical significance (P＜0.05).
Referring to Fig. 6, under the alkaline stress, the number of blade of all super dry treatment seed seedling exceeds control treatment equally, is 112.5～150% of contrast, and gap has statistical significance (P＜0.05).
(c) super dry treatment to the influence of seedling leaf area under neutral environment and the salt alkaline stress referring to Fig. 7 to 9.
Referring to Fig. 7, under the neutral environment, exceed contrast 4.83～14.64% in the super dry treatment seedling leaf area in mass fraction water content 5.72～5.18% scopes, gap has statistical significance (P＜0.05).
Referring to Fig. 8, under the salt stress, be 123.80～165.65% of contrast in the super dry treatment seedling leaf area of 5.72～4.97% of mass fraction water content, significant difference is (P＜0.05) significantly.
Referring to Fig. 9, under the alkaline stress, significantly exceed control treatment in the super dry treatment seedling leaf area of 6.36～4.97% of mass fraction water content, be 116.26～167.95% of contrast, significant difference is (P＜0.05) significantly.
(d) super dry treatment to the influence of seedling chlorophyll content under neutral environment and the salt alkaline stress referring to Figure 10 to 12.
Referring to Figure 10, under the neutral environment, contrast the seedling height in the super dry treatment seedling chlorophyll content of 5.72～4.59% of mass fraction water content, be 4.83～12.70% of contrast.
Referring to Figure 11, under the salt stress, exceed control treatment 10.01～71.31% in the super dry treatment seed seedling chlorophyll content of 7.09～4.97% of mass fraction water content, gap has statistical significance (P＜0.05).
Referring to Figure 12, under the alkaline stress, exceed control treatment 121.77～140.53% in the super dry treatment seed seedling chlorophyll content of 6.36～5.46% of mass fraction water content, gap has statistical significance (P＜0.05).
Above experimental data shows, east, Gansu Province alfalfa seed adopts the super super dried mode that reduces seed moisture content fast of handling of intervening of silica gel, can realize improving the seedling effect that impermeabilisation is coerced in saline-alkali environment, improve the resistance of seed vitality and gained seedling, and realize the water savingization and the drought resistance of clover cultivation by the mode that improves speed of emergence, the regularity of emerging.It is simple, energy-conservation, the effective seed preconditioning technique that can prolong the seed storage life-span.
Claims (8)
1. an alfalfa seed preprocess method that improves seedling vigor and saline and alkaline resistance is characterized in that: comprise the processing of super dry treatment of seed and rehydration;
Described super dry treatment is: seed is inserted in the gas permeability mesh bag, evenly be positioned in the drier that fills with silica gel, described silica gel is to dry to constant weight and be cooled to the oven dry silica gel of room temperature, and changed silica gel every 23～25 hours, with drying silica gel with the at room temperature dry 12～216h of seed, make seed reduce to 4.59～7.09% so that mass fraction is moisture under sealing state, dried seed seals with aluminium-foil paper, place the drier that fills oven dry silica gel, room temperature storage 6～365 days;
Described rehydration is treated to: will surpass the closed environment that the seed after dry treatment is also preserved places relative moisture to raise successively successively before the sowing, and improve seed moisture content gradually.
2. the alfalfa seed preprocess method of raising according to claim 1 seedling vigor and saline and alkaline resistance is characterized in that: changed silica gel in the described super dry treatment every 24 hours.
3. the alfalfa seed preprocess method of raising seedling vigor according to claim 1 and saline and alkaline resistance is characterized in that, the environment that the super dry seeds rehydration after the storage is handled is followed successively by: CaCl
2Closed environment, the NH of the relative moisture 35.0% that saturated solution is regulated
4The closed environment of the relative moisture 100% that the closed environment of the relative moisture 70% that the Cl saturated solution is regulated and distilled water are regulated, multiple time for water treatment is 24～26 hours in each closed environment.
4. according to the alfalfa seed preprocess method of claim 1 or 2 or 3 described raising seedling vigor and saline and alkaline resistance, it is characterized in that the multiple time for water treatment in each closed environment of the super dry seeds after the storage is 24 hours.
5. the alfalfa seed preprocess method of raising according to claim 1 seedling vigor and saline and alkaline resistance is characterized in that: during super dry treatment, described seed is 1:10 with the mass ratio of drying silica gel.
6. the alfalfa seed preprocess method of raising seedling vigor according to claim 1 and saline and alkaline resistance is characterized in that: silica gel is changed once every 24h, and described oven dry silica gel is to constant weight and cooled silica gel through 120 ℃ of hyperthermia dryings.
7. the alfalfa seed preprocess method of raising seedling vigor according to claim 1 and saline and alkaline resistance, it is characterized in that: be 72～120h the drying time of described seed, dry back seed is moisture in mass fraction to be 5.18～5.72%.
8. the pretreating method for seed of raising seedling vigor according to claim 1 and saline and alkaline resistance, it is characterized in that: described seed is an alfalfa seed.
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|---|---|---|---|---|
| CN102498788A (en) * | 2011-11-11 | 2012-06-20 | 云南省农业科学院园艺作物研究所 | Method for improving drought resistance of pepper seedlings |
| CN102715003A (en) * | 2012-06-27 | 2012-10-10 | 上海交通大学 | Plantation method for obtaining high-content high-yield sweet wormwood in saline-alkali soil |
| CN103229762A (en) * | 2013-04-18 | 2013-08-07 | 中国热带农业科学院分析测试中心 | Leucaena leucocephala seed ultra-dry preservation method |
| CN104541879A (en) * | 2014-12-31 | 2015-04-29 | 重庆昶信农业有限公司 | Planting technology of artemisia apiacea |
| CN107241938A (en) * | 2017-08-18 | 2017-10-13 | 叶世青 | A kind of xeothermic biography pest and disease damage method of committing genocide of high temperature of super-dry rice seeds |
| CN107409532A (en) * | 2017-07-21 | 2017-12-01 | 广西柳城县成霖农业科技有限公司 | A kind of seed-soaking method for improving Soybean Resistance Saline Alkali Stress ability |
| CN109601262A (en) * | 2018-12-05 | 2019-04-12 | 中国农业科学院北京畜牧兽医研究所 | The implantation methods of clover drought tolerance are improved by regulation endogenous hormones |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102498788A (en) * | 2011-11-11 | 2012-06-20 | 云南省农业科学院园艺作物研究所 | Method for improving drought resistance of pepper seedlings |
| CN102715003A (en) * | 2012-06-27 | 2012-10-10 | 上海交通大学 | Plantation method for obtaining high-content high-yield sweet wormwood in saline-alkali soil |
| CN103229762A (en) * | 2013-04-18 | 2013-08-07 | 中国热带农业科学院分析测试中心 | Leucaena leucocephala seed ultra-dry preservation method |
| CN104541879A (en) * | 2014-12-31 | 2015-04-29 | 重庆昶信农业有限公司 | Planting technology of artemisia apiacea |
| CN107409532A (en) * | 2017-07-21 | 2017-12-01 | 广西柳城县成霖农业科技有限公司 | A kind of seed-soaking method for improving Soybean Resistance Saline Alkali Stress ability |
| CN107241938A (en) * | 2017-08-18 | 2017-10-13 | 叶世青 | A kind of xeothermic biography pest and disease damage method of committing genocide of high temperature of super-dry rice seeds |
| CN109601262A (en) * | 2018-12-05 | 2019-04-12 | 中国农业科学院北京畜牧兽医研究所 | The implantation methods of clover drought tolerance are improved by regulation endogenous hormones |
| CN109601262B (en) * | 2018-12-05 | 2020-11-17 | 中国农业科学院北京畜牧兽医研究所 | Planting method for improving drought tolerance of alfalfa by regulating endogenous hormone |
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