CN102186875A - 较高细胞密度的澄清方法 - Google Patents

较高细胞密度的澄清方法 Download PDF

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Publication number
CN102186875A
CN102186875A CN2009801405074A CN200980140507A CN102186875A CN 102186875 A CN102186875 A CN 102186875A CN 2009801405074 A CN2009801405074 A CN 2009801405074A CN 200980140507 A CN200980140507 A CN 200980140507A CN 102186875 A CN102186875 A CN 102186875A
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CN
China
Prior art keywords
cell
culture fluid
biological agents
supernatant layer
cell culture
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
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CN2009801405074A
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English (en)
Chinese (zh)
Inventor
G·扎尔比斯-帕帕斯托伊特西斯
M·C·库策乌斯基
E·贝尔彻席尔默
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DSM IP Assets BV
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DSM IP Assets BV
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Publication date
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Publication of CN102186875A publication Critical patent/CN102186875A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
CN2009801405074A 2008-10-17 2009-10-16 较高细胞密度的澄清方法 Pending CN102186875A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US10632008P 2008-10-17 2008-10-17
US61/106,320 2008-10-17
EP08167506 2008-10-24
EP08167506.8 2008-10-24
PCT/EP2009/063565 WO2010043700A1 (en) 2008-10-17 2009-10-16 Clarification process at higher cell density

Publications (1)

Publication Number Publication Date
CN102186875A true CN102186875A (zh) 2011-09-14

Family

ID=40367660

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2009801405074A Pending CN102186875A (zh) 2008-10-17 2009-10-16 较高细胞密度的澄清方法

Country Status (7)

Country Link
EP (1) EP2346891A1 (fi)
JP (1) JP2012505863A (fi)
KR (1) KR20110085980A (fi)
CN (1) CN102186875A (fi)
AU (1) AU2009305343A1 (fi)
CA (1) CA2739501A1 (fi)
WO (1) WO2010043700A1 (fi)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012030512A1 (en) 2010-09-03 2012-03-08 Percivia Llc. Flow-through protein purification process
SG10201808061WA (en) 2013-09-30 2018-10-30 Janssen Vaccines & Prevention Bv Method for the clarification of high density crude cell culture harvest
SG10201911134QA (en) 2015-06-05 2020-01-30 Grace W R & Co Adsorbent bioprocessing clarification agents and methods of making and using the same

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030170810A1 (en) * 2001-09-18 2003-09-11 Masoud Vedadi Methods and apparatuses for purification
WO2007123961A2 (en) * 2006-04-20 2007-11-01 Wyeth Purification processes for isolating purified vesicular stomatitis virus from cell culture

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030170810A1 (en) * 2001-09-18 2003-09-11 Masoud Vedadi Methods and apparatuses for purification
WO2007123961A2 (en) * 2006-04-20 2007-11-01 Wyeth Purification processes for isolating purified vesicular stomatitis virus from cell culture

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
FRANK RISKE ET AL.,: ""The use of chitosan as a flocculant in mammalian cell culture dramatically improves clarification throughput without adversely impacting monoclonal antibody recovery"", 《JOURNAL OF BIOTECHNOLOGY》 *

Also Published As

Publication number Publication date
JP2012505863A (ja) 2012-03-08
CA2739501A1 (en) 2010-04-22
EP2346891A1 (en) 2011-07-27
AU2009305343A1 (en) 2010-04-22
KR20110085980A (ko) 2011-07-27
WO2010043700A1 (en) 2010-04-22

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