CN102174382A - System and method for monitoring bioaerosol in real time - Google Patents
System and method for monitoring bioaerosol in real time Download PDFInfo
- Publication number
- CN102174382A CN102174382A CN2011100204503A CN201110020450A CN102174382A CN 102174382 A CN102174382 A CN 102174382A CN 2011100204503 A CN2011100204503 A CN 2011100204503A CN 201110020450 A CN201110020450 A CN 201110020450A CN 102174382 A CN102174382 A CN 102174382A
- Authority
- CN
- China
- Prior art keywords
- sample
- amplifier
- lock
- sampling
- electrode
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000012544 monitoring process Methods 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000005070 sampling Methods 0.000 claims abstract description 43
- 244000005700 microbiome Species 0.000 claims abstract description 16
- 238000001514 detection method Methods 0.000 claims abstract description 12
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 12
- 238000012545 processing Methods 0.000 claims abstract description 6
- 230000000694 effects Effects 0.000 claims abstract description 5
- 230000005540 biological transmission Effects 0.000 claims abstract description 4
- 239000000523 sample Substances 0.000 claims description 74
- 239000002070 nanowire Substances 0.000 claims description 22
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 21
- 229910052710 silicon Inorganic materials 0.000 claims description 21
- 239000010703 silicon Substances 0.000 claims description 21
- 238000000018 DNA microarray Methods 0.000 claims description 19
- 239000002184 metal Substances 0.000 claims description 18
- 230000005686 electrostatic field Effects 0.000 claims description 12
- 239000012212 insulator Substances 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 230000005611 electricity Effects 0.000 claims description 4
- 238000012805 post-processing Methods 0.000 claims description 4
- 230000008859 change Effects 0.000 claims description 3
- 230000007613 environmental effect Effects 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 2
- 238000011897 real-time detection Methods 0.000 claims 1
- 241000894006 Bacteria Species 0.000 abstract description 5
- 241000700605 Viruses Species 0.000 abstract description 5
- 239000013566 allergen Substances 0.000 abstract 1
- 239000012678 infectious agent Substances 0.000 abstract 1
- 241000712461 unidentified influenza virus Species 0.000 description 10
- 238000005516 engineering process Methods 0.000 description 9
- 206010022000 influenza Diseases 0.000 description 6
- 230000003321 amplification Effects 0.000 description 5
- 238000003199 nucleic acid amplification method Methods 0.000 description 5
- 230000004544 DNA amplification Effects 0.000 description 3
- YFTHZRPMJXBUME-UHFFFAOYSA-N tripropylamine Chemical compound CCCN(CCC)CCC YFTHZRPMJXBUME-UHFFFAOYSA-N 0.000 description 3
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 2
- 125000003172 aldehyde group Chemical group 0.000 description 2
- 125000003277 amino group Chemical group 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
- 230000005684 electric field Effects 0.000 description 2
- 230000005669 field effect Effects 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 239000002086 nanomaterial Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000001069 Raman spectroscopy Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 239000002210 silicon-based material Substances 0.000 description 1
- BEOOHQFXGBMRKU-UHFFFAOYSA-N sodium cyanoborohydride Chemical compound [Na+].[B-]C#N BEOOHQFXGBMRKU-UHFFFAOYSA-N 0.000 description 1
- 241000894007 species Species 0.000 description 1
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Abstract
The invention discloses a system and method for monitoring bioaerosol in real time. The system comprises a sample acquisition and transmission unit, a signal detection and processing unit and a data output and display unit, wherein, the sample acquisition and delivery unit comprises a sampler (1), a sample delivery pipe (2), a peristaltic pump (5) and a sampling medium container; the signal detection and processing unit comprises a preamplifier (6), a phase-locked amplifier (7) and a biological chip (3) integrated with a biosensor (5); and the data output and display unit comprises a computer host and a display connected with the phase-locked amplifier (7). The system and method provided by the invention can be used for monitoring infectious agents in the air, comprising viruses, bacteria, and allergen and the like in real time, can play a role in preventing the biological risks, such as large flu epidemic situation and bioterrorism activity and the like, and also can be used for dynamically representing the changes of microorganism in the air and the like.
Description
Technical field
The present invention relates to a kind of bioaerosol real-time monitoring system and method, mainly can be used for monitoring in real time airborne pathogenic former (comprising virus, bacterium, anaphylactogen etc.), thereby when taking precautions against bio-hazard such as large-scale influenza epidemic situation, bio-terrorism activity, play a role, also can be used for dynamically characterizing the variation of air microorganism etc.
Background technology
The breathing of bioaerosol exposes and has caused a lot of health problems, and large-scale in recent years flu outbreak has caused huge lives and properties and financial loss such as the influenza of the H1N1 of SARS in 2003 and 2009.In order to take precautions against and resist these danger better, press for a kind of can be to virus, the bacterium technology of monitoring in real time in the air, yet this is a technical barrier for a long time.In the past few years a lot of technology were once attempted being used for realizing the on-line monitoring of bioaerosol, comprising mass-spectrometric technique, surface enhanced Raman technology, cell streaming instrument, also have based on the Ultraviolet Aerodynamic ParticleSizer (UVAPS) of fluorescence etc., but these technology are difficult to realize to the examination of microorganism in the air or exist very high problems such as false positive.Though the appearance of gene amplification makes the accuracy of the airborne microorganism of monitoring improve greatly, these systems are difficult to and Sampling techniques etc. are integrated into automatic monitoring system.
Performances such as the power of the uniqueness that nano material/structural table reveals, electricity, heat, light, magnetic, can realize the performance boundary of new function or breakthrough conventional device, realize having the nano-device and the system of new principle or high-performance (as highly sensitive, reduce power consumption, lower noise).Silicon nanowires is a kind of as one dimension Nano structure, has as many excellent properties different with the body silicon materials such as electron transport, field emission characteristic, surfactivity and quantum limit, thereby has very application prospects aspect the making of low-dimensional nano-device.Can control its semi-conductor conductivity effectively by mixing.In recent years, the field-effect transistor based on nano wire successfully has been applied in the influenza virus and several bioproteins of parallel monitoring of monitoring in the water.But these researchs are primarily aimed at the microorganism monitoring in the liquid medium, also do not report the research that utilizes silicon nanowires field-effect transistor on-line monitoring bioaerosol at present.
Summary of the invention
At the problem of above-mentioned existence, the present invention is intended to by technology such as integrated bio sampling of aerosol, microflow control technique and biological detection, feeble signal amplifications, realizes the former on-line monitoring that causes a disease in the air has been filled up the blank in this field.
Technical scheme provided by the invention is as follows:
Scheme 1: the aerocolloidal system of a kind of real-time monitoring bio (as shown in Figure 1), it is characterized in that, comprise three parts:
-sample collecting and supply unit comprise sampling thief 1, sample delivery pipe 2, peristaltic pump 9, collection containers 10 and sampling media container 11; The conveying of sample is finished in the collection that described sampling thief 1 is realized bioaerosol then by sample delivery pipe 2 under the effect of peristaltic pump 9 by sampling media, and through micro-fluidic 4, biochip 3, be transported to collection containers 10 at last;
-signal detection and processing unit comprise prime amplifier 6, lock-in amplifier 7, are integrated with the biochip 3 and micro-fluidic 4 of silicon nano-wire biological sensor 5;
Output of-data and display unit comprise the host computer and the indicating meter that link to each other with lock-in amplifier 7; Be equipped with on the described host computer and be used for the data that lock is put are transmitted the software that control, signal post-processing and result show.
Scheme 2: a kind of preferred realization as scheme 1 is characterized in that described sampling thief 1 is electrostatic field sampling thief (as shown in Figure 3), comprising: dome electrode 101, circular electrode 102, sample collecting groove 104; Wherein, dome electrode 101 is fixed on the insulator foot 106, and circular electrode 102 is positioned at the sphere center position of insulator foot 106 first ball shaped electrodes 101, and described sample collecting groove 104 is positioned at the top of circular electrode 102; A plurality of air intlets 111 are arranged at dome electrode 101 tops; On insulator foot 106, be provided with two airs 110; In sample collecting groove 104, there is 108 and one of a sample delivery outlet to gather liquid input aperture 109.
Scheme 3: as a kind of preferred realization of scheme 1, it is characterized in that, in described signal detection and the processing unit, the microelectrode of metal probe contact devices, connect input electrical signal by the metal probe lead-in wire and be applied on the chip electrode, draw detected signal simultaneously and input to surveying instrument (prime amplifier 6 and lock-in amplifier 7); Wherein, two metal probes connect the source-drain electrode of some silicon nano-wire biological sensors respectively, be connected with cable between metal probe and lock-in amplifier 7 and the prime amplifier 6, a metal probe links to each other with the reference output of lock-in amplifier 7, and another probe output is connected to the voltage input of lock-in amplifier 7 through prime amplifier 6.With reference to the output signal maximum amplitude is 5V, and highest frequency is 100kHz.
Scheme 4: a kind of preferred realization as scheme 1, it is characterized in that the electrode surface material of described biochip 3 is Au, for reducing the contact resistance between probe and microelectrode, selecting the probe tip material for use is the composition metal of Au.
Scheme 5: a kind of preferred realization as scheme 4 is characterized in that the needle point radius-of-curvature of described probe tip is 25 μ m.
Scheme 6: a kind of preferred realization as scheme 2 is characterized in that, in described sample collecting and the supply unit, the pump head of peristaltic pump 9 is two-tube output, one end of one of them flexible pipe connects sampling media (for example, sterilized water), and the other end connects sample collecting groove 104 input interfaces of sampling thief 1; One end of another flexible pipe connects sample collecting groove 104 output interfaces, and the other end is connected on the inlet of micro-fluidic 4 on the biochip 3.Certain rotating speed is set, in the peristaltic pump operational process air sample that collects is transported to biochip surface from the collection groove of sampling thief by micro-fluidic 4, by micro-fluidic 4 outlet sample delivery is arrived sample collecting bottle 10 at last, simultaneously the sampling media of gathering in the groove is replenished.Can change the speed that sample flow is crossed chip by the rotating speed of regulating peristaltic pump.
Scheme 7: as a kind of preferred realization of scheme 1, it is characterized in that, in described data output and the display unit, in order to show detection signal in real time, consider follow-up possible multi-channel detection simultaneously, adopt extendible GPIB card and private cable to connect lock-in amplifier 7 and host computer; Cable links to each other with the RS232 serial ports of lock-in amplifier 7; On the described host computer computer software is installed, this software realizes that Data Transmission Controlling, signal post-processing, result that lock is put show; Program interface comprises the input reference voltage value, sampling number/sampling time, and magnification, gauge dial shows and the measured electricity in back that converts is led change curve, and can preserve the data that real-time monitoring is surveyed.
Scheme 8: a kind of preferred realization as scheme 7 is characterized in that described computer software has the function that triggers warning when detecting thing to be checked.Even under unattended situation, also can realize real-time monitoring like this to microorganism.
The present invention provides a kind of method of utilizing scheme 1 described system to monitor in real time simultaneously, and scheme is as follows:
Scheme 9: a kind of scheme 1 described system that utilizes carries out the aerocolloidal method of real-time monitoring bio, it is characterized in that, comprises the steps:
A), biochip 3 is carried out specific antibody modify at the object microorganism A that is monitored (for example: virus, bacterium, anaphylactogen etc.);
B) start-up system realizes the continuous sampling of environmental sample, the real-time conveying of sample, the real-time demonstration of silicon nanowires conductivity;
C) when detected object microorganism A is arranged in the air sample that collects, in indicating system, demonstrate the electroconductibility generation obvious variation of silicon nanowires, the electroconductibility that is higher than the correspondence of silicon nanowires itself or negative air sample far away, based on the specificity of antibody, the phenomenon that electric conductivity value significantly changes can confirm the existence of detected object microorganism A.
Scheme 10: a kind of preferred realization as scheme 9, it is characterized in that, after detecting microorganism A existence, report to the police by launch computer.Warning in the time of can realizing unmanned like this.
Beneficial effect of the present invention: this invention provides the novel method in bioaerosol on-line monitoring field, realized utilizing biochip on-line monitoring bioaerosol first, monitoring time is significantly shorter than existing most of technology, realized real-time monitoring to influenza virus in the air, not only can screen kind, but also can be quantitative.In medical and health organization, drome, critical point, airport and strick precaution influenza and bio-terrorism activity, there is important use to be worth.
Description of drawings
The online bioaerosol Monitoring systems of Fig. 1 structural representation.
The airborne influenza virus of Fig. 2 on-line monitoring (H3N2 hypotype) sample.
Fig. 3 electrostatic field sampling thief structural representation.
Wherein, mark is described as follows among Fig. 1:
| 1: the electrostatic field sampling thief | 2: the sample delivery pipe |
| 3: biochip | 4: micro-fluidic |
| 5: biosensor (integrated a plurality of silicon nanowires on it) | 6, |
| 7, lock-in |
8, display routine |
| 9, |
10, sample collection bottle |
| 11, sampling media container | 12, vacuum pump |
| 13, high-voltage DC power supply |
Mark is described as follows among Fig. 3:
| 101: dome electrode | 102: circular electrode |
| 103: the impressed voltage interface | 104: the sample collecting groove |
| 105: particle charger | 106: insulator foot |
| 107: the retaining screw mother | 108: the sample delivery outlet |
| 109: gather the liquid input aperture | 110: air |
| 111: air intlet |
Embodiment
With reference to structure shown in Figure 1, the present invention has realized a concrete system: electrostatic field sampling thief 1 connects biochip 3 by sample delivery pipe 2, microfluidic channel 4 is arranged on biochip 3, integrated a plurality of silicon nano-wire biological sensors 5 on it, on biochip 3, also be connected with signal preamplifier 6 and signal lock-in amplifier 7 (signal preamplifier 6 also links to each other with signal lock-in amplifier 7), computer LabView signal display routine 8 is installed on the signal lock-in amplifier 7, is used to show monitoring result; Electrostatic field sampling thief 1 links to each other with a peristaltic pump 9 by another root sample delivery pipe 2, in order to realize the real-time transmission of sampling media; The two ends of peristaltic pump 9 connect sample collection bottle 10 and sample solution bottle 11 respectively; On the base of electrostatic field sampling thief, be connected to a vacuum pump 12, in order to drive the circulation of air in sampling thief; Electrostatic field sampling thief 1 links to each other with high-voltage DC power supply 13, in order to drive sampling thief work.
Described electrostatic field sampling thief 1 is a kind of air sampler based on electrostatic field (as shown in Figure 3), it is characterized in that, comprising: dome electrode 101, circular electrode 102, sample collecting groove 104; Wherein, dome electrode 101 is fixed on the insulator foot 106, and circular electrode 102 is positioned at the sphere center position of insulator foot 106 first ball shaped electrodes 101, and described sample collecting groove 104 is positioned at the top of ball shaped electrode 102; A plurality of air intlets 111 are arranged at dome electrode 101 tops; On insulator foot 106, be provided with a plurality of airs 110; In sample collecting groove 104, there is 108 and one of a sample delivery outlet to gather liquid input aperture 109.103 is the impressed voltage interface among the figure, and 105 is particle charger, and 107 for being fixed to dome electrode 101 on the nut on the insulator foot 106.This electrostatic field sampling thief utilizes electrostatic field to make in the sample space in the air electrically charged microorganism under electric field action, concentrates along direction of an electric field to be deposited on the hemisphere center relatively in the liquid medium than the zonule, thereby realizes the purpose of sampling.
Described signal preamplifier 6 is LI76 (NF), and signal lock-in amplifier 7 is LI5640 (NF), and vacuum pump 12 is the SKC pump, and biochip 3 is made of silicon nanowire array, and data-interface is AgilentPCI-GPIB82350B.
The electrode surface material of sensor chip 5 is Au, and for reducing the contact resistance between probe and microelectrode, selecting the needle point material for use is the compound Au probe (the needle point radius-of-curvature is 25 μ m) of Au.Be connected with the bnc interface cable between metal probe and lock-in amplifier and the prime amplifier.One of them metal probe links to each other with the reference output of lock-in amplifier, and another metal probe is connected to the voltage input of lock-in amplifier through prime amplifier.With reference to the output signal maximum amplitude is 5V, and highest frequency is 100kHz.
Monitoring method following (is example with influenza virus H3N2):
(1), biochip is carried out specific antibody (H3N2 Antibody of Influenza) modify at first at the object of being monitored (H3N2);
(2) start-up system realizes the continuous sampling of environmental sample, the real-time conveying of sample, the real-time demonstration of silicon nanowires conductivity;
(3) when in the air sample that collects influenza virus being arranged, obvious variation can take place in the electroconductibility of silicon nanowires, the electroconductibility that is higher than the correspondence of silicon nanowires itself or negative air sample far away, based on the specificity of antibody, the phenomenon that electric conductivity value significantly changes can confirm the existence of influenza virus.
Biochip is carried out specific antibody (H3N2 Antibody of Influenza) modify, mainly finish by 4 steps: the first step, under the room temperature, nano-device and glutaraldehyde (5% glutaraldehyde, pH=8) reaction is 1 hour, and (10mM, pH=8) flushing is 5 minutes to use phosphate buffered saline buffer again.In second step, H3N2 antibody (0.1mg/ml antibody-solutions, pH=8 comprise 4mM sodiumcyanoborohydride) wraps by nano-device, and reaction conditions is 4 ℃, 14 hours.In this process, the aldehyde groups combination on the amino group of antibody end and nano-device surface.In the 3rd step, (10mM, pH=8) flushing is 5 minutes with phosphate buffered saline buffer.In the 4th step, (the 100mM Tri N-Propyl Amine pH=8) wraps by nano-device 2 hours time to utilize Tri N-Propyl Amine solution.Utilize the amino group sealing nano-device surface of Tri N-Propyl Amine not have aldehyde groups with antibodies.After corresponding microorganism occurring in the air, it is condensing to be sampled the device collection, enters the chip detection part, and Ag-Ab immunity association reaction takes place, and the bio signal of appearance is converted to electrical signal through preposition amplification and phase-locked amplification, has promptly realized on-line monitoring.
Fig. 2 is the partial data of on-line monitoring influenza virus (H3N2 hypotype), according to the information among the figure, when having influenza virus (H3N2 hypotype) in the air, total system can show that in 1-3 branch clock time the significant electricity of silicon nanowires leads variation, meanwhile, result according to gene amplification shows that the sample correspondence that influenza virus concentration is high in the air higher silicon nanowires electroconductibility.This system not only can be used for monitoring virus, and can carry out on-line monitoring to biotic components such as airborne bacterium and anaphylactogens equally.On detection time,, usually need 2-3 hour such as gene amplification considerably beyond other conventional art.
To sum up, the present invention has following advantage:
(1) the present invention's integrated by to technology such as silicon nano-wire biological sensor, micro-fluidic, air continuous sampling and the phase-locked amplifications of signal, formed the bioaerosol real-time monitoring system, result of study shows that when having influenza virus to exist in the air, this system can show early warning signal in 1-3 minute;
(2) this system integration the technology of different field comprise the amplification and the demonstration of environment sampling, micro-fluidic delivery system, nanotechnology and detection signal;
(3) this system is an auto-real-time monitoring system that does not need artificial supervision, can realize the real-time examination of living species in the air and quantitatively.
Claims (10)
1. the aerocolloidal system of real-time monitoring bio is characterized in that, comprises three parts:
-sample collecting and supply unit comprise sampling thief (1), sample delivery pipe (2), peristaltic pump (9), sampling media container (11), collection containers (10); Described sampling thief (1) is realized the collection to bioaerosol, under the effect of peristaltic pump (9), finish the conveying of sample then by sample delivery pipe (2) by sampling media, and, be transported to collection containers (10) at last through micro-fluidic (4), biochip (3);
-signal detection and processing unit comprise prime amplifier (6), lock-in amplifier (7), are integrated with the biochip (3) of silicon nano-wire biological sensor (5);
Output of-data and display unit comprise the host computer and the indicating meter that link to each other with lock-in amplifier (7); Be equipped with on the described host computer and be used for the data that lock is put are transmitted the software that control, signal post-processing and result show.
2. the system as claimed in claim 1 is characterized in that, described sampling thief (1) is the electrostatic field sampling thief, comprising: dome electrode (101), circular electrode (102), sample collecting groove (104); Wherein, dome electrode (101) is fixed on the insulator foot (106), and circular electrode (102) is positioned at the sphere center position of first ball shaped electrode of insulator foot (106) (101), and described sample collecting groove (104) is positioned at the top of circular electrode (102); A plurality of air intlets (111) are arranged at dome electrode (101) top; On insulator foot (106), be provided with two airs (110); A sample delivery outlet (108) and a collection liquid input aperture (109) are arranged in sample collecting groove (104).
3. the system as claimed in claim 1, it is characterized in that, in described signal detection and the processing unit, the microelectrode of metal probe contact devices, connect input electrical signal by the metal probe lead-in wire and be applied on the chip electrode, draw detected signal simultaneously and input to surveying instrument; Wherein, two metal probes connect the source-drain electrode of some silicon nano-wire biological sensors respectively, be connected with cable between metal probe and lock-in amplifier (7) and the prime amplifier (6), a metal probe links to each other with the reference output of lock-in amplifier (7), and another metal probe is connected to the voltage input of lock-in amplifier (7) through prime amplifier (6).
4. the system as claimed in claim 1 is characterized in that, the electrode surface material of described biochip (3) is Au, and metal probe needle point material is the composition metal of Au.
5. system as claimed in claim 4 is characterized in that, the needle point radius-of-curvature of described metal probe needle point is 25 μ m.
6. system as claimed in claim 2, it is characterized in that in described sample collecting and the supply unit, the pump head of peristaltic pump (9) is two-tube output, one end of one of them flexible pipe connects sampling media, and the other end connects sample collecting groove (104) input interface of sampling thief (1); One end of another flexible pipe connects sample collecting groove (104) output interface, and the other end is connected in the outlet of micro-fluidic (4) on the biochip (3).
7. the system as claimed in claim 1 is characterized in that, in described data output and the display unit, adopts extendible GPIB card and private cable to connect lock-in amplifier (7) and host computer; Cable links to each other with the RS232 serial ports of lock-in amplifier (7); On the described host computer computer software is installed, this software realizes that Data Transmission Controlling, signal post-processing, result that lock is put show; Program interface comprises the input reference voltage value, sampling number/sampling time, and magnification, gauge dial shows and the measured electricity in back that converts is led change curve, and can preserve the data of real-time detection.
8. system as claimed in claim 7 is characterized in that, described computer software has the function that triggers warning when detecting thing to be checked.
9. one kind is utilized claim 1 described system to carry out the aerocolloidal method of real-time monitoring bio, it is characterized in that, comprises the steps:
A), biochip (3) is carried out specific antibody modify at the object microorganism A that is monitored;
B) start-up system realizes the continuous sampling of environmental sample, the real-time conveying of sample, the real-time demonstration of silicon nanowires conductivity;
C) when detected object microorganism A is arranged in the air sample that collects, in indicating system, demonstrate the electroconductibility generation obvious variation of silicon nanowires, the electroconductibility that is higher than the correspondence of silicon nanowires itself or negative air sample far away, based on the specificity of antibody, the phenomenon that electric conductivity value significantly changes can confirm the existence of detected object microorganism A.
10. method as claimed in claim 9 is characterized in that, after detecting microorganism A existence, is reported to the police by launch computer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110020450 CN102174382B (en) | 2011-01-18 | 2011-01-18 | System and method for monitoring bioaerosol in real time |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110020450 CN102174382B (en) | 2011-01-18 | 2011-01-18 | System and method for monitoring bioaerosol in real time |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102174382A true CN102174382A (en) | 2011-09-07 |
| CN102174382B CN102174382B (en) | 2013-05-08 |
Family
ID=44517640
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110020450 Expired - Fee Related CN102174382B (en) | 2011-01-18 | 2011-01-18 | System and method for monitoring bioaerosol in real time |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102174382B (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106525733A (en) * | 2016-10-25 | 2017-03-22 | 西安交通大学 | Quick detection device and method for microbial aerosol in air |
| CN108362754A (en) * | 2018-01-19 | 2018-08-03 | 北京大学 | Biomarker on-line detecting system and method in a kind of expiratory air |
| CN109564144A (en) * | 2016-10-26 | 2019-04-02 | 株式会社岛津制作所 | Circulate bottle and Autosampler |
| CN111529225A (en) * | 2020-06-12 | 2020-08-14 | 吉林大学 | Ambulance with function of immediately detecting new coronavirus in air |
| CN111763614A (en) * | 2020-07-24 | 2020-10-13 | 北京大学 | Online bioaerosol monitoring system and method based on ATP (adenosine triphosphate) biochemical luminescence |
| CN112649412A (en) * | 2020-12-29 | 2021-04-13 | 北京鼎蓝科技有限公司 | Bio-aerosol early warning device based on ATP bioluminescence technology |
| CN113295653A (en) * | 2021-05-20 | 2021-08-24 | 北京航空航天大学 | Real-time detection system and method for aerogel pathogens |
| CN114594208A (en) * | 2022-03-07 | 2022-06-07 | 谱瑞前海(深圳)智能科技有限公司 | Real-time detection device for virus spread to epidemic disease in air environment |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001079522A2 (en) * | 2000-04-14 | 2001-10-25 | Caliper Technologies Corp. | Screening assay methods and systems using target pooling |
| CN1460855A (en) * | 2003-06-13 | 2003-12-10 | 中国科学院生物物理研究所 | Microfluid biological sensor chip device and its application |
-
2011
- 2011-01-18 CN CN 201110020450 patent/CN102174382B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001079522A2 (en) * | 2000-04-14 | 2001-10-25 | Caliper Technologies Corp. | Screening assay methods and systems using target pooling |
| CN1460855A (en) * | 2003-06-13 | 2003-12-10 | 中国科学院生物物理研究所 | Microfluid biological sensor chip device and its application |
Non-Patent Citations (2)
| Title |
|---|
| 张惠力等: "《静电场采样装置对室内空气中过敏原采集效率的研究》", 《环境监测管理与技术》 * |
| 钱乐等: "《嗜肺军团气溶胶静电场采样技术初步研究》", 《环境与健康杂志》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106525733A (en) * | 2016-10-25 | 2017-03-22 | 西安交通大学 | Quick detection device and method for microbial aerosol in air |
| CN109564144A (en) * | 2016-10-26 | 2019-04-02 | 株式会社岛津制作所 | Circulate bottle and Autosampler |
| CN108362754A (en) * | 2018-01-19 | 2018-08-03 | 北京大学 | Biomarker on-line detecting system and method in a kind of expiratory air |
| CN108362754B (en) * | 2018-01-19 | 2020-10-09 | 北京大学 | Online detection system and method for biomarkers in exhaled breath |
| CN111529225A (en) * | 2020-06-12 | 2020-08-14 | 吉林大学 | Ambulance with function of immediately detecting new coronavirus in air |
| CN111763614A (en) * | 2020-07-24 | 2020-10-13 | 北京大学 | Online bioaerosol monitoring system and method based on ATP (adenosine triphosphate) biochemical luminescence |
| CN111763614B (en) * | 2020-07-24 | 2023-06-20 | 北京大学 | On-line bioaerosol monitoring system and method based on ATP biochemical luminescence |
| CN112649412A (en) * | 2020-12-29 | 2021-04-13 | 北京鼎蓝科技有限公司 | Bio-aerosol early warning device based on ATP bioluminescence technology |
| CN113295653A (en) * | 2021-05-20 | 2021-08-24 | 北京航空航天大学 | Real-time detection system and method for aerogel pathogens |
| CN114594208A (en) * | 2022-03-07 | 2022-06-07 | 谱瑞前海(深圳)智能科技有限公司 | Real-time detection device for virus spread to epidemic disease in air environment |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102174382B (en) | 2013-05-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102174382B (en) | System and method for monitoring bioaerosol in real time | |
| Hamada et al. | Development of rapid oral bacteria detection apparatus based on dielectrophoretic impedance measurement method | |
| Shen et al. | Integrating silicon nanowire field effect transistor, microfluidics and air sampling techniques for real-time monitoring biological aerosols | |
| Tan et al. | Development of an automated electrostatic sampler (AES) for bioaerosol detection | |
| CN103168241B (en) | Virus detection device and virus detection method | |
| CN202956299U (en) | Portable biological aerosol enrichment and quick detection and analysis device | |
| CN108387410A (en) | The wet wall Cyclonic mass flow air sampler of electrostatic field and gravity centrifugation enhancing | |
| CN102109423B (en) | Electrostatic field-based air sampler and sampling method thereof | |
| CN102435654A (en) | Viral disease diagnosis device and method based on field effect transistor | |
| CN208270267U (en) | A kind of mass flow air sampler | |
| US11754523B2 (en) | Detection device and method for coronavirus and influenza virus | |
| CN102559486B (en) | Full-automatic air microbe sampler | |
| CN106442649B (en) | A method of 1,5- dewatered grape sugar alcohol is detected based on EIS structure electrochemical biosensor | |
| TW202142864A (en) | Bioaerosol detection apparatus | |
| CN112683977A (en) | Novel coronavirus detection module and method based on multi-target-site antibody combination | |
| CN115093943B (en) | Biological aerosol enrichment device by centrifugal impact method and cell exposure and application thereof | |
| CN104502303A (en) | Sub-THz nano-biosensor for quickly frame-detecting bacteria and detection method thereof | |
| CN106482988A (en) | A kind of Portable high-flow air sampler | |
| Ma et al. | Rapid detection of airborne protein from Mycobacterium tuberculosis using a biosensor detection system | |
| CN202401061U (en) | Full-automatic air microbe sampler | |
| CN108362754A (en) | Biomarker on-line detecting system and method in a kind of expiratory air | |
| CN105158310B (en) | A kind of micro-fluidic detection chip and its application based on micro-porous electrode | |
| Rastmanesh et al. | On-Site Bioaerosol Sampling and Airborne Microorganism Detection Technologies | |
| Li et al. | Electrochemical biosensors based on carbon nanomaterials for diagnosis of human respiratory diseases | |
| CN202631299U (en) | Liquid impact type microbial air monitoring system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20181106 Address after: 100085 D405, block D, 9 Sandi street, Haidian District, Beijing. Patentee after: Beijing Ding LAN Technology Co.,Ltd. Address before: 100085 F block 606, 9 on the 3rd Street, Haidian District, Beijing. Patentee before: Beijing Zhong LAN Technology Co.,Ltd. Effective date of registration: 20181106 Address after: 100085 F block 606, 9 on the 3rd Street, Haidian District, Beijing. Patentee after: Beijing Zhong LAN Technology Co.,Ltd. Address before: 100871 No. 5, the Summer Palace Road, Beijing, Haidian District Patentee before: Peking University |
|
| TR01 | Transfer of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130508 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |