CN102168016B - Active dry yeast protectant and application thereof - Google Patents
Active dry yeast protectant and application thereof Download PDFInfo
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- CN102168016B CN102168016B CN2011100360893A CN201110036089A CN102168016B CN 102168016 B CN102168016 B CN 102168016B CN 2011100360893 A CN2011100360893 A CN 2011100360893A CN 201110036089 A CN201110036089 A CN 201110036089A CN 102168016 B CN102168016 B CN 102168016B
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Abstract
The invention relates to an active dry yeast dehydration protectant and application thereof and belongs to the technical field of microorganism products and food additives. The active dry yeast protectant comprises the following components: glyceryl monostearate, sorbitan monostearate, sorbitan monooleate (Span80) and glycerin. The active dry yeast protectant can obviously increase the living cell rate of active dry yeast to 86 to 90 percent, obviously increase the preserving rate of active dry yeast to 88 to 90 percent and obviously increase the fermentation ability of active dry yeast to 530 to 550mL/h.
Description
Technical field
The present invention relates to agent of a kind of active dry yeast dehydration protection and application thereof, belong to microbial product and technical field of food additives.
Background technology
Active dry yeast is to be processed through the granulating and drying dehydration by fresh yeast or title yeast cake, and the activity of dry yeast is represented with fermenting power usually.
Generally speaking, the 3.5g fresh yeast could produce the 1g active dry yeast, but with regard to active (with regard to the fermenting power), the 1g active dry yeast only is equivalent to the 2-3g fresh yeast: check is surveyed viable cell and is generally 80-90% after the rehydration.Fresh yeast is processed active dry yeast, and after the storage and the reconstitution process before the use of certain hour, the certain activity loss is arranged.
The factor that influences the dry yeast vigor has the following aspects:
1, chemical constitution is to the influence of dry yeast vigor
The nitrogen phosphorus in the yeast and the content of trehalose have very big influence to the activity of active dry yeast and storage.The cell nitrogen content is high, and not only dead easily in drying process, the cell composition is also oxidized easily in storage, thereby causes cell to deposit the inactivation death in the process in group.If the nitrogen content of control cell, then the total carbohydrates content in the yeast cell increases, and particularly the amount of trehalose increases, and helps the stability of cell in dry and storage process like this.But if nitrogen content is too low, then zymic enzyme system is abundant, and it is lower to ferment during use, and particularly to high sugar-fermenting, fermentation activity is very poor, this shows, when control cell nitrogen content, comprehensively package stability and fermentation activity are considered.Help improving fermentation speed for the baker's active dried yeast high nitrogen content, but the exsiccant condition must be confirmed accurately.
Higher content of trehalose helps prolonging the storage period of active dry yeast.And the height of the content of phosphorus accumulates for trehalose and the activity of cell nitrogen content level and enzyme all has certain influence.The content of the trehalose of general active dry yeast reaches about 14% of dry-matter, and the phosphorus content of yeast cell is (with P
2O
5Calculate) when being the 30-40% of nitrogen content, product has package stability preferably.These moitys of active dry yeast can be regulated through control culture condition and cultivation moity in the culturing process of yeast cell.But different yeast strains has certain difference.
2, moisture is to the influence of dry yeast vigor
The yeast cell water cut of state of nature is 70-80%, and wherein combination water content is 15-20%, and free water content is 50-60%; The yeast cell of state of nature processed has the certain activity loss behind the active dry yeast; And the degree of loss is relevant with the moisture content in the yeast cell, and in general, moisture is low more; Help the storage of active dry yeast more, because the oxidized speed of yeast material reduces with the reduction of moisture content.But then, moisture content is low more, and active loss is just big more in drying and the reconstitution process.The moisture content height is unfavorable for preserving, and storage loss are big, and drying and rehydration loss are little.Therefore, the moisture content of active dry yeast must be considered the stability of storage, considers the loss of activity of dry and reconstitution process again.
When moisture content during at 15-30%, because the not forfeiture basically of zymic combined moisture generally can not influence vigor and fermenting power, but the active dry yeast of this high-moisture can only refrigerate.When the moisture of active dry yeast was reduced to 15%, yeast had certain activity to change, and the stability of at room temperature storing strengthens greatly, in dry and reconstitution process, the small portion loss of activity is arranged.When the moisture content of active dry yeast was 7.5-8.3%, active dry yeast can be stored at normal temperatures, and preservation period can reach more than half a year, in dry and reconstitution process, the forfeiture of parts of fine cytoactive will be arranged.For not adding protectant active dry yeast, when moisture content is lower than 7.5%, the loss of activity in dry and reconstitution process will increase greatly, therefore not add protectant active dry yeast, and its moisture content should not be lower than 7.5%.For adding protectant active dry yeast, its moisture content can be reduced to 4-5%, and preservation period can reach more than 1 year.
3, packaging means is to the active influence of dry yeast
Active dry yeast is at lay up period, and active loss is owing to the oxidized result of cellular constituent, and anaerobic and low moisture content are then because the maintenance of cytoactive.Same active dry yeast product, the Packaging Method that is adopted is different, and its preservation period is just different, in general, adopts vacuum or nitrogen-filled packaging, and its preservation period prolongs 1-3 doubly than ordinary packing.
4, protective material is to the active influence of dry yeast
Protective material is before the yeast drying granulation, evenly sneaks in the fresh yeast activity of protection yeast cell in drying, storage.Do not use protectant active dry yeast product, its moisture content can not be lower than 7.5%, otherwise active loss will be very big, and adding protectant yeast has low water activity preferably.
5, drying temperature is to the influence of yeast activity
Drying temperature is too high, makes cell inactivation dead easily, and temperature is crossed to hang down prolonged time of drying, and product moisture increases.Rate of drying also must be controlled within the specific limits, and rate of drying is too slow, and prolong time of drying, and the loss of activity that yeast cell causes because of oxidation in drying process increases; Rate of drying is too fast, and yeast cell is causing cell membrane damage in the dehydration rapidly easily, makes cell inactivation dead.
Summary of the invention
The present invention is directed to the deficiency of prior art, a kind of active dry yeast protective material and application thereof are provided.
The term explanation:
Air flow: be meant the volume ratio of the amount of air capacity that PM feeds and yeast culture liquid, unit is vvm.
Cell concn: be meant in the fermented liquid or zymic mass concentration in the yeast-lactic.
A kind of active dry yeast protective material is characterized in that composition comprises glyceryl monostearate,, sorbitan mono-oleic acid ester and glycerine.
The weight percent content of said glyceryl monostearate is: 40-44%; The weight percent content of is: 25-30%; The weight percent content of sorbitan mono-oleic acid ester is: 24-26%, the weight percent content of glycerine is: 4-6%.
Preferably, the weight percent content of glyceryl monostearate is: 43%, and the weight percent content of is: 28%, the weight percent content of sorbitan mono-oleic acid ester is: 25%, the weight percent content of glycerine is: 4%.
The application of above-mentioned active dry yeast protective material in dry yeast is preserved is characterized in that step is following:
(1) the yeast fermentation medium centrifugal is separated, obtain to contain the yeast-lactic of solid substance 16-17% cell concn;
(2) in the yeast-lactic that step (1) makes, add above-mentioned active dry yeast protective material, addition is the 2-4% of dry matter weight in the yeast-lactic, through filtering, makes the yeast slurry of water cut 28-30% again;
(3) yeast slurry that step (2) is made is dried to moisture 5.0-5.5% under 40-45 ℃ of condition.
Described yeast is a yeast saccharomyces cerevisiae, and the code name that preferred Institute of Microorganism, Academia Sinica sells is the yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) of AS 2.146.
The preparation of the yeast fermentation nutrient solution in the step (1) can also can adopt following method to make by prior art:
To the seed culture fluid that in the fermentation basic medium of sterilization, inserts after inoculation culture, regulate pH5.2,30-35 ℃ of control culture temperature; Stream sugared soln and nutrient salt solution; The control total fermentation time is 18-21h, in the fermentation system glucose quality percentage composition below 0.5%, pH5.2; Air flow 1.2-2.5vvm, making cell concn is the yeast fermentation nutrient solution of 4.5-5.5%.
Preferably, control culture temperature 30 ℃ of culture temperature when fermentation time 1-8h, culture temperature is 32 ℃ during fermentation time 9-15h, fermentation time 16h 35 ℃ of culture temperature during to fermentation ends; The control air flow is 2.5vvm when fermentation time 1-15h, is 1.2vvm at fermentation time 16h during to fermentation ends; The glucose quality percentage composition is at 0.30-0.50% in the control fermentation system.
Preferably, said fermentation basic medium component is following, is volume percent:
The steeping water 20% that contains solid substance 28wt%, the glucose solution 1% of 250g/L, nutrient salt solution 5%, water 74%;
Wherein, nutrient salt solution contains for every liter: KH
2PO
413.0g, (NH
4)
2SO
494.16g, MgSO
47H
2O 1.4g, ZnSO
47H
2O 0.28g.
Preferably, described seed culture fluid, every liter contains following component:
Glucose 20g, KH
2PO
41g, (NH
4)
2SO
45g, MgSO
47H
2O 0.2g, ZnSO
47H
2O 0.05g, pH5.4.
Preferably, the sugar soln that adds in the process of stream is the glucose solution of the 250g/L that is made into by glucose; The nutrient salt solution that stream adds in the process contains for every liter: KH
2PO
413.0g, (NH
4)
2SO
494.16g, MgSO
47H
2O 1.4g, ZnSO
47H
2O0.28g.
In the above-mentioned steps (2) the protectant addition of active dry yeast be in the yeast-lactic dry matter weight 2.5%.
Yeast conservation agent in the method for the present invention; Can protect yeast cell its activity of protection under the condition of dehydration; This is that cytolemma is well disperseed because this protective material can make yeast cell under the condition of dehydration, when avoiding dewatering because of the distortion of cytolemma; And tenuigenin is flowed out, and cause necrocytosis.
Technical characterstic and excellent results of the present invention are following:
1. active dry yeast protective material of the present invention can make the active dry yeast living cell rate significantly improve, and can reach 86-90%.
2. active dry yeast protective material of the present invention can make the active dry yeast storage rate significantly improve, and can reach 88-90%.
3. active dry yeast protective material of the present invention can make the active dry yeast fermenting power significantly improve, and can reach 530-550mL/h.
Description of drawings
Fig. 1 is the sem photograph that uses dry yeast after the active dry yeast protective material among the embodiment 1;
Fig. 2 is to use the sem photograph of dry yeast after the Sp60 protective material.
Embodiment
Below in conjunction with embodiment, technical scheme of the present invention is done further elaboration, but institute of the present invention protection domain is not limited only to this.Used bacterial classification and substratum are among the embodiment 1-4:
Yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) bacterial classification is available from Institute of Microorganism, Academia Sinica, and DSMZ of Institute of Microorganism, Academia Sinica bacterial classification catalogue code name: AS 2.146;
Seed culture fluid: glucose 20g, KH
2PO
41g, (NH
4)
2SO
45g, MgSO
47H
2O 0.2g, ZnSO
47H
2O 0.05g, pH5.4, zero(ppm) water 1000mL.
Fermentation basic medium component is following, is volume percent:
The steeping water 20% that contains solid substance 28wt%, the glucose solution 1% of 250g/L, nutrient salt solution 5%, water 74%;
Wherein, nutrient salt solution contains for every liter: KH
2PO
413.0g, (NH
4)
2SO
494.16g, MgSO
47H
2O 1.4g, ZnSO
47H
2O 0.28g.
The sugar soln that stream adds in the process is the glucose solution of the 250g/L that is made into by glucose; The nutrient salt solution that stream adds in the process contains for every liter: KH
2PO
413.0g, (NH
4)
2SO
494.16g, MgSO
47H
2O 1.4g, ZnSO
47H
2O 0.28g.
The preparation of yeast fermentation nutrient solution
With carrying out reality jar sterilization in the 50L fermentor tank that 25L fermentation basic medium is housed, sterilising conditions is 120 ℃, 30min.When being cooled to 32 ℃ then, insert the seed culture fluid 3L after inoculation culture, regulate pH5.2; 30 ℃ of control culture temperature; Stream sugared soln and nutrient salt solution, the control total fermentation time is 21h, nutrient salt solution added in fermentation ends in preceding 5 hours; The stream dosage of sugar soln is according to the yeast growth situation, makes that glucose content is controlled at below 0.5% always in the fermentation system.In the fermenting process, control pH5.2, air flow 1.2-2.5vvm, making cell concn is the yeast fermentation nutrient solution of 4.5-5.5%.
Culture temperature control: culture temperature is 30 ℃ when fermentation time 1-8h, and culture temperature is 32 ℃ during 9-15h, and culture temperature is 35 ℃ during 16-21h.
Air flow control: air flow 2.5vvm when fermentation time 1-15h, air flow 1.2vvm when fermentation 16-21h.
Control sugar soln stream dosage makes glucose content 0.30-0.50% volume ratio in the fermentation system; The pH regulator agent is 10wt%Na
2CO
3Solution.
With the above-mentioned yeast fermentation nutrient solution that makes,, obtain containing the yeast-lactic of solid substance 16-17%, as the raw material of 1-4 in the embodiment of the invention through centrifugal, washing, centrifugal, washing more again.
Active dry yeast living cell rate, storage rate and fermenting power measuring method are according to the method for GB/T 20886-2007 food-processing with the yeast regulation.
Embodiment 1
A kind of active dry yeast protective material, component is following, all is weight percentage:
Glyceryl monostearate 40%, 30%, sorbitan mono-oleic acid ester 24%, glycerine 6%.With mentioned component, be made into 20% emulsion with 90 ℃ of zero(ppm) water.Use after being cooled to 45 ℃.
In yeast-lactic, add above-mentioned active dry yeast protective material; The weight percent that accounts for dry-matter in the yeast-lactic is 2.5%, fully stirs 40min, refilters the collection yeast slurry; Water cut 28-30%; Extruder grain becomes the cylindrical particle of 0.1mm diameter, utilizes 45 ℃ of air, dries to moisture 5.0-5.5%.Measuring the active dry yeast living cell rate respectively is 85%; The active dry yeast storage rate is: 87%; Active dry yeast fermenting power (CO
2) be 530mL/h, its sem photograph is as shown in Figure 1.
Reference examples
Use the Sp60 emulsifying agent that moistens magnificent foodstuff additive Ltd available from the Guangzhou, under identical experiment condition, do control experiment, measuring the active dry yeast living cell rate respectively is 82%; The active dry yeast storage rate is 83%; Active dry yeast fermenting power (CO
2) be 515mL/h, its sem photograph is as shown in Figure 2.
Embodiment 2
Like embodiment 1 described active dry yeast protective material, difference is:
Glyceryl monostearate 44%, 25%, sorbitan mono-oleic acid ester 26%, glycerine 5%.
Measuring the active dry yeast living cell rate respectively is 87%; The active dry yeast storage rate is: 89%; Active dry yeast fermenting power (CO
2) be 530mL/h.
Embodiment 3
Like embodiment 1 described active dry yeast protective material, difference is:
Glyceryl monostearate 43%, 28%, sorbitan mono-oleic acid ester 25%, glycerine 4%.
Measuring the active dry yeast living cell rate respectively is 89%; The active dry yeast storage rate is: 91%; Active dry yeast fermenting power (CO
2) be 545mL/h.
Embodiment 4
Like embodiment 1 described active dry yeast protective material, difference is:
Glyceryl monostearate 42%, 27%, sorbitan mono-oleic acid ester 26%, glycerine 5%.
Measuring the active dry yeast living cell rate respectively is 86%; The active dry yeast storage rate is: 88%; Active dry yeast fermenting power (CO
2) be 550mL/h.
Embodiment 5
Utilize the active dry yeast protective material, component is following: (all being weight percentage):
Glyceryl monostearate 40%, 30%, sorbitan mono-oleic acid ester 24%, glycerine 6%.With mentioned component, be made into 20% emulsion with 90 ℃ of zero(ppm) water.Use after being cooled to 45 ℃.
Different debaryomyces hansenii 1312 (Hansenula anomala1312) bacterial strain that the bacterial classification that this example laboratory adopts is sold available from China National Academy of Food & Fermentation Industries.The zymic cultural method is identical with the yeast saccharomyces cerevisiae cultural method of embodiment 1-4, and obtains to contain the yeast-lactic of solid substance 16-17%.In yeast-lactic, add above-mentioned active dry yeast protective material; The weight percent that accounts for dry-matter in the yeast-lactic is 2.5%, fully stirs 40min, refilters the collection yeast slurry; Water cut 28-29%; Extruder grain becomes the cylindrical particle of 0.1mm diameter, utilizes 45 ℃ of air, dries to moisture 5.0-5.5%.Measuring the active dry yeast living cell rate respectively is 87%; The active dry yeast storage rate is: 81%.
Claims (11)
1. an active dry yeast protective material is characterized in that, is grouped into by following one-tenth: glyceryl monostearate,, sorbitan mono-oleic acid ester and glycerine;
The weight percent content of said glyceryl monostearate is: 40-44%; The weight percent content of is: 25-30%; The weight percent content of sorbitan mono-oleic acid ester is: 24-26%, the weight percent content of glycerine is: 4-6%.
2. active dry yeast protective material as claimed in claim 1; It is characterized in that; The weight percent content of said glyceryl monostearate is: 43%; The weight percent content of is: 28%, and the weight percent content of sorbitan mono-oleic acid ester is: 25%, the weight percent content of glycerine is: 4%.
3. the application of the said active dry yeast protective material of claim 1 in dry yeast is preserved.
4. application as claimed in claim 3 is characterized in that step is following:
(1) the yeast fermentation medium centrifugal is separated, obtain to contain the yeast-lactic of solid substance 16-17% cell concn;
(2) in the yeast-lactic that step (1) makes, add above-mentioned active dry yeast protective material, addition is the 2-4% of dry matter weight in the yeast-lactic, through filtering, makes the yeast slurry of water cut 28-30% again;
(3) yeast slurry that step (2) is made is dried to moisture 5.0-5.5% under 40-45 ℃ of condition;
Said cell concn is meant in the fermented liquid or the mass concentration of yeast saccharomyces cerevisiae in the yeast-lactic.
5. application as claimed in claim 4 is characterized in that, described yeast is a yeast saccharomyces cerevisiae.
6. application as claimed in claim 4 is characterized in that, the yeast fermentation nutrient solution in the step (1) adopts following method to make:
To the seed culture fluid that in the fermentation basic medium of sterilization, inserts after inoculation culture, regulate pH5.2,30-35 ℃ of control culture temperature; Stream sugared soln and nutrient salt solution; The control total fermentation time is 18-21h, in the fermentation system glucose quality percentage composition below 0.5%, pH5.2; Air flow 1.2-2.5vvm, making cell concn is the yeast fermentation nutrient solution of 4.5-5.5%.
7. application as claimed in claim 6 is characterized in that, control culture temperature 30 ℃ of culture temperature when fermentation time 1-8h, and culture temperature is 32 ℃ during fermentation time 9-15 h, fermentation time 16h 35 ℃ of culture temperature during to fermentation ends; The control air flow is 2.5vvm when fermentation time 1-15h, is 1.2vvm at fermentation time 16h during to fermentation ends; The glucose quality percentage composition is at 0.30-0.50% in the control fermentation system.
8. application as claimed in claim 6 is characterized in that, said fermentation basic medium component is following, is volume percent:
The steeping water 20% that contains solid substance 28wt%, the glucose solution 1% of 250g/L, nutrient salt solution 5%, water 74%;
Wherein, nutrient salt solution contains for every liter: KH
2PO
413.0g, (NH
4)
2SO
494.16g, MgSO
47H
2O 1.4g, ZnSO
47H
2O 0.28g.
9. application as claimed in claim 6 is characterized in that, the sugar soln that said stream adds in the process is the glucose solution of the 250g/L that is made into by glucose; The nutrient salt solution that stream adds in the process contains for every liter: KH
2PO
413.0g, (NH
4)
2SO
494.16g, MgSO
47H
2O 1.4g, ZnSO
47H
2O 0.28g.
10. application as claimed in claim 6 is characterized in that, described seed culture fluid, and every liter contains following component:
Glucose 20g, KH
2PO
41g, (NH
4)
2SO
45g, MgSO
47H
2O 0.2g, ZnSO
47H
2O 0.05g, pH5.4.
11. application as claimed in claim 4 is characterized in that, in the above-mentioned steps (2) the protectant addition of active dry yeast be in the yeast-lactic dry matter weight 2.5%.
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| CN102792985A (en) * | 2012-09-12 | 2012-11-28 | 济南瑞成生物工程有限公司 | Composite active bread dried yeast and preparation method thereof |
| CN103141666B (en) * | 2013-03-28 | 2014-04-16 | 山东轻工业学院 | Method for producing microbe feed probiotics by using white spirit vinasse |
| CN103497896B (en) * | 2013-10-16 | 2016-03-09 | 济南瑞丰生物工程有限公司 | A kind of dehydration pit mud functional bacteria protective material and application thereof |
| CN103911304A (en) * | 2013-11-07 | 2014-07-09 | 领绿生物镇江有限公司 | Efficient yeast protective agent and usage method thereof |
| CN109234324A (en) * | 2018-11-22 | 2019-01-18 | 浙江华康药业股份有限公司 | A kind of method that cellulose is converted into ethyl alcohol in furfuraldehyde waste slag |
| CN111557409A (en) * | 2020-05-26 | 2020-08-21 | 山西实美食品有限公司 | Processing method of functional millet leavened cake powder |
| CN111748481A (en) * | 2020-06-23 | 2020-10-09 | 天津科技大学 | Active soy sauce dry yeast prepared by using candida and method for fermenting soy sauce by using active soy sauce dry yeast |
| CN112094747B (en) * | 2020-09-28 | 2023-02-24 | 武汉新华扬生物股份有限公司 | Preparation method of kluyveromyces marxianus active dry yeast |
| CN112708564A (en) * | 2020-12-28 | 2021-04-27 | 酆敏 | Preservation solution for saccharomycete strains and preparation method and use method thereof |
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| CN101045903A (en) * | 2006-03-29 | 2007-10-03 | 邵峰 | Probiotic freeze-drying process |
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| CN101045903A (en) * | 2006-03-29 | 2007-10-03 | 邵峰 | Probiotic freeze-drying process |
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