CN102154837A - Inactivation method for polished cellulolytic enzyme - Google Patents
Inactivation method for polished cellulolytic enzyme Download PDFInfo
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- CN102154837A CN102154837A CN 201010609732 CN201010609732A CN102154837A CN 102154837 A CN102154837 A CN 102154837A CN 201010609732 CN201010609732 CN 201010609732 CN 201010609732 A CN201010609732 A CN 201010609732A CN 102154837 A CN102154837 A CN 102154837A
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Abstract
The invention relates to an inactivation method for polished cellulolytic enzyme, which is different from the traditional inactivation method for cellulolytic enzyme. The inactivation method is characterized in that a conventional process is used for carrying out cellulolytic enzyme polishing treatment on cotton knitted fabric; after the completion of polishing treatment, the fabric is taken out, is quickly centrifuged and dehydrated so as to lead the rate of liquid entrainment in a cloth cover to be controlled to be 80 to 100%, and then the fabric is roasted under a temperature of 80 DEG C till the rate of liquid entrainment in the fabric is about 20 to 30%; and finally the fabric is roasted under a temperature of 130 to 180 DEG C for 2-5min. In the process, molecular chains of the cellulolytic enzyme adsorbed on the pure cotton knitted fabric irreversibly extend and gather so as to cause the activation loss of the cellulolytic enzyme, wherein the inactivated cellulolytic enzyme is remained on the surface of the fabric, and hydrophobic parts in the structure of the inactivated cellulolytic enzyme lead the hydrophilic nature of the surface of the pure cotton to change.
Description
Technical field
The present invention relates to a kind of cellulase deactivation new method, especially for the cellulase ablation method of pure cotton knitted fabric polishing.
Background technology
Cellulase is the general name of catalyzing hydrolysis cellulose, glucogenic enzyme, and the enzyme system by the multicomponent enzyme is formed comprises endoglucanase, exoglucanase and beta-glucosidase, and the three is collaborative to play hydrolysis to cellulose.The cellulose restriction endonuclease can be along cellulose molecular chain hydrolysis β-1 at random, the 4-glycosidic bond, thus increased the terminal quantity of cellulose molecular chain, for the effect of excision enzyme provides the position; The cellulose excision enzyme then can only form cellobiose or glucose from the plain molecule of an end-grain cutting protruding end of cellulose molecular chain, can expose more cellulose molecular chain simultaneously for the restriction endonuclease effect; The effect of beta-glucosidase then is that cellobiose is hydrolyzed into glucose.
Cellulose treatment can obviously be improved the wearability of cellulose fabrics such as cotton, fiber crops, improves its added value, and the action condition gentleness, and is low to environmental hazard, caused the extensive concern of textile printing and dyeing circle in recent years.Cellulase is used the most successful field and is comprised indigo cowboy's biological fabric washing and the arrangement of pure cotton fabric biopolishing in textile printing and dyeing industry.Cellulase involved in the present invention is mainly used in COTTON FABRIC is carried out the biopolishing processing.Surface of cotton fabric is if exist more fine hair can influence its outward appearance, the hydrolyzation catalysis effect of cellulase can make fine hair or fiber reduction, by cooperating with the effective of mechanical agitation power, surface of cotton fabric fine hair can be removed, reach the purpose of biopolishing, the effect of biopolishing arrangement has persistence, and this also is better than other chemical finishing.
At present the microorganism of production of cellulose enzyme belongs to fungi mostly, uses more have wooden mould, aspergillus, humic head mold etc.When pure cotton fabric being polished, should select high impulsive force equipment in reel dye machine, spraying machine, the rotating cage type rinsing machine etc. for use with cellulase.The selection of bath raio is also careful, satisfy and handle the free-pouring needs of fabric, but too high bath raio then can have dilution to enzyme concentration, increases processing cost.Enzyme dosage is controlled at 1.0-3.0% (by handled thing weight) usually, to special heavily fabric, also should suitably improve consumption.In order to guarantee best polishing effect, must be optimized treatment process parameter such as pH, temperature, time, under optimum condition, use as far as possible.
It is generally acknowledged that cellulase is finished after the polishing without any favourable value.Continue catalyzing hydrolysis and cause the follow-up strength loss of fabric excessive for fear of the cellulase that remains on the cloth cover, also will pass through inactivation treatment, make cellulase generation irreversible denaturation inactivation, the forfeiture catalytic capability.At present, printing and dyeing producer extensively adopts the mode that improves treatment fluid temperature and pH value to make the cellulase inactivation, make the plain enzyme of most of residual devitalizing fiberses by eccysis, discharging by the brute force washing then, fabric face did not keep any cellulase protein substantially after washing was finished.
Summary of the invention
The purpose of this invention is to provide the strong polishing cellulase ablation method of a kind of actual operation, can rapidly and efficiently finish the deactivation of cellulase, cellulase after the deactivation can be retained in processed fabric face again, hydrophobic part in the plain enzymatic structure of devitalizing fibers can change the surface nature of COTTON FABRIC, its hydrophilicity is descended, with the then therefore enhancing of binding ability of lyophobic dust.
Basic process of the present invention is described below: the pure cotton knitted fabric through the pre-treatment of scouringing and bleaching is carried out the plain enzyme polishing of conventional fibre, the waste liquid of draining after polished the finishing dealing with, fabric is taken out back centrifugal dehydration rapidly, the cloth cover liquid carrying rate is controlled at about 80-100%, then fabric is dried by the fire to the liquid carrying rate 20-30% 80 ℃ of temperature, at last fabric is baked 2-5min under 130-180 ℃ of temperature, finish inactivation process.
Pure cotton knitted fabric must carry out sufficient kiering and bleaching before carrying out polishing, remove cotton fiber self with various natural impuritys and the exogenous impurity of introducing at weaving process.When carrying out polishing, high impact forces equipment in needing to adopt is because cellulase only just can be given full play to polishing action under the cooperation of certain mechanical force.Polishing generally continues 20-60min, after finishing fabric taken out and use the centrifuge centrifugal dehydration, the cloth cover liquid carrying rate is controlled at about 80-100%, then fabric is dried by the fire to the liquid carrying rate 20-30% in the temperature about 80 ℃, at last fabric is baked 2-5min under 130-180 ℃ of temperature, wherein temperature is preferably 150-180 ℃.In general, baking temperature is high more, and required time is just long more.As can baking 5min at 150 ℃, and if bake at 180 ℃, the time is controlled at about 2min, in order to avoid fabric is caused damage.High temperature bakes in the process, and irreversible stretching, extension and gathering take place Cellulase Molecules chain adsorbed on the pure cotton fabric, causes the forfeiture of cellulase catalytic activity, but still is adsorbed on the cotton fiber.
The invention provides a kind of cellulase deactivation new method, this method is not carried out deactivation to polishing the back cellulase in treatment fluid, but make the cellulase inactivation that is adsorbed on the cotton fiber by the mode that high temperature bakes, thereby the cellulase behind the inactivation is kept on fiber, the existence that keeps cellulase protein can make the surface hydrophilic performance of cotton fiber change, and plays the effect of cotton fiber being carried out surface modification.
The specific embodiment
Further specify the present invention below by embodiment and Comparative Examples.
Embodiment
At first with cellulase to the pure cotton knitted fabric (200g/m after scouringing and bleaching
2) carry out polishing, every cloth specimen weight is 20g, adopts the bit machine to test, other process conditions are as follows:
Polishing cellulase (50mg/ml) 18ml/L
JFC (nonionic wetting agent) 0.1g/L
PH (acetic acid adjusting) 5.5
50 ℃ of temperature
Time 30min
Bath raio 20: 1
After polishing is finished, fabric taken out and with centrifuge dewatering to liquid carrying rate 80%, then fabric is dried by the fire to liquid carrying rate 25% in 80 ℃ of baking ovens, under higher temperature, bake then, actual conditions is as shown in table 1:
Table 1 fabric bakes condition
Bake after inactivation finishes, the hydrophily of inactivation rate, cellulase reservation amount and the fabric face of cellulase protein on the test cloth specimen, the result is as shown in table 2:
Table 2 cellulase inactivation rate, fabric hydrophilic and reservation cellulase amount
As can be seen, baking temperature improves or bakes the inactivation that time lengthening all helps cellulase.If baking temperature is 150 ℃, the time of baking needs 5min, is increased to 180 ℃ if bake, and then only needs 2min can make its complete deactivation.Generally do not recommend 130 ℃ and bake,, need time expand, the performances such as feel of pure cotton fabric are had adverse effect but bake overlong time if think complete deactivation because inactivation rate is lower under this temperature.With method of the present invention to the cellulase deactivation after, the part cellulase is retained in fabric face, reservation amount and baking temperature are irrelevant; From hydrophily, the plain enzyme of devitalizing fibers that is retained in fabric face descends the hydrophily of fabric face, and level of deactivation is high more, and hydrophily descends obvious more, shows the change degree of surface of cotton fabric character big more.
Comparative Examples
Carry out inactivation treatment according to conventional method.According to polishing condition among the embodiment pure cotton knitted fabric is handled, with soda ash treatment fluid pH value is transferred to 9 and temperature risen to 85 ℃ after finishing, behind the insulation 15min treatment fluid is discharged, inject the clear water of same amount then, after cleaning 15min fabric is taken out dehydration, at last 80 ℃ of oven dry down.Its test result is also listed in the table 2, handles to guarantee the cellulase complete deactivation as can be seen with this method, but the cellulase behind the inactivation can't remain on the COTTON FABRIC, thereby also can't utilize the plain enzyme of this devitalizing fibers that COTTON FABRIC is carried out surface modification.Show that after tested fabric testing time of dripping is 1.3 seconds after traditional inactivation treatment, suitable with the back pure cotton knitted fabric hydrophilicity (1.2 seconds) of scouringing and bleaching, show that the surface hydrophilic character of pure cotton fabric takes place obviously to change after the plain enzyme-deactivating of traditional fibre is handled.
Claims (2)
1. a polishing cellulase ablation method is different from the plain enzyme-deactivating mode of traditional fibre, can guarantee that cellulase activity completely loses, and again the cellulase after the deactivation is retained in surface of cotton fabric, and the surface of cotton fabric hydrophilic nmature is changed.It is characterized in that technology is crossed pure cotton knitted fabric at first routinely carries out the cellulase polishing, after polished the finishing dealing with fabric is taken out and centrifugal dehydration rapidly, the cloth cover liquid carrying rate is controlled at about 80-100%, then fabric is dried by the fire to the liquid carrying rate 20-30% 80 ℃ of temperature, at last fabric is baked 2-5min under 130-180 ℃ of temperature, finish inactivation process.
2. according to the described cellulase ablation method of claim 1, wherein baking temperature is 150-180 ℃.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010106097322A CN102154837B (en) | 2010-12-22 | 2010-12-22 | Inactivation method for polished cellulolytic enzyme |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010106097322A CN102154837B (en) | 2010-12-22 | 2010-12-22 | Inactivation method for polished cellulolytic enzyme |
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| CN102154837A true CN102154837A (en) | 2011-08-17 |
| CN102154837B CN102154837B (en) | 2012-07-04 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102505532A (en) * | 2011-09-26 | 2012-06-20 | 青岛大学 | Method for promoting pigment dyeing by using cellulase |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03241076A (en) * | 1990-02-13 | 1991-10-28 | Chugoku Senko Kk | Production of jeans product |
| CN1570260A (en) * | 2004-04-30 | 2005-01-26 | 中国农业科学院麻类研究所 | Grass cloth processing process |
| JP2006207095A (en) * | 2005-01-31 | 2006-08-10 | Koike Tateami Senkosho:Kk | Method for treating cotton-silk blended yarn |
| CN101555664A (en) * | 2009-05-20 | 2009-10-14 | 西南大学 | Method for increasing cellulose enzyme activity in processing ramie fabrics |
| CN101818396A (en) * | 2010-03-31 | 2010-09-01 | 长沙市欧林雅家纺有限责任公司 | Anti-pilling bamboo fiber textile products in bamboo fiber field and preparation method thereof |
-
2010
- 2010-12-22 CN CN2010106097322A patent/CN102154837B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03241076A (en) * | 1990-02-13 | 1991-10-28 | Chugoku Senko Kk | Production of jeans product |
| CN1570260A (en) * | 2004-04-30 | 2005-01-26 | 中国农业科学院麻类研究所 | Grass cloth processing process |
| JP2006207095A (en) * | 2005-01-31 | 2006-08-10 | Koike Tateami Senkosho:Kk | Method for treating cotton-silk blended yarn |
| CN101555664A (en) * | 2009-05-20 | 2009-10-14 | 西南大学 | Method for increasing cellulose enzyme activity in processing ramie fabrics |
| CN101818396A (en) * | 2010-03-31 | 2010-09-01 | 长沙市欧林雅家纺有限责任公司 | Anti-pilling bamboo fiber textile products in bamboo fiber field and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102505532A (en) * | 2011-09-26 | 2012-06-20 | 青岛大学 | Method for promoting pigment dyeing by using cellulase |
| CN102505532B (en) * | 2011-09-26 | 2013-05-01 | 青岛大学 | Method for promoting pigment dyeing by using cellulase |
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| CN102154837B (en) | 2012-07-04 |
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