CN102154406A - Method for producing bacterial cellulose by taking high-concentration methanol-containing waste water as raw material - Google Patents
Method for producing bacterial cellulose by taking high-concentration methanol-containing waste water as raw material Download PDFInfo
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- CN102154406A CN102154406A CN2011100095968A CN201110009596A CN102154406A CN 102154406 A CN102154406 A CN 102154406A CN 2011100095968 A CN2011100095968 A CN 2011100095968A CN 201110009596 A CN201110009596 A CN 201110009596A CN 102154406 A CN102154406 A CN 102154406A
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Abstract
The invention relates to a method for producing bacterial cellulose by taking high-concentration methanol-containing waste water as a raw material. The method comprises the following steps of: inoculating gluconacetobacter xylinus in a culture solution for fermentation; and shaping, bleaching and drying the bacterial cellulose to obtain a finished product; the method is characterized in that: high-concentration methanol-containing wastewater from petroleum and coal chemical industries is used as a carbon source and process water in the culture solution; and a preparation method of the bacterial cellulose comprises the following steps of: adding a nitrogen source and inorganic salt into the high-concentration methanol-containing wastewater from the petroleum or the coal chemical industry; and adjusting pH value to be between 6.8 and 7.0. The method has the advantages that: production cost of the bacterial cellulose can be reduced, recycling of the high-concentration methanol-containing wastewater from the petroleum and coal chemical industry is realized, and the problem of environmental pollution can be solved.
Description
Technical field
The present invention relates to a kind of methanol rectification waste water that produces with oil, coal chemical industry field is the method for raw material production bacteria cellulose.
Background technology
Methyl alcohol is a kind of important chemical material, and the high concentration methanol waste water that oil, coal chemical industry industry produce mainly is meant in the methanol production process, the vinasse that rectification process is got rid of.This wastewater temperature height has the strong impulse smell, and main component is methyl alcohol, ethanol, higher alcohols and aldehydes, also contains some long-chain compounds.COD and BOD
5Be generally 8000～20000mg/L and 5000～10000mg/L, its BOD
5/ COD is higher, belongs to high concentrated organic wastewater.Oil, coal resources have not only been wasted in any discharging of such waste water, and the biology in the environment is had toxic action, cause soil and phreatic pollution, and are also very outstanding to atmospheric pollution problem.
Bacteria cellulose (bacterial cellulose, be called for short BC) be by a kind of ultra micro pure cellulose of microorganism synthetic, the bacteria cellulose molecule is independently filamentary fibers form, other polyose impurity such as xylogen, hemicellulose that undope, so bacteria cellulose not only has the premium properties of plant cellulose, also has the purity height, the degree of crystallinity height, unique physical and chemical properties and mechanical propertys such as water-absorbent is strong, and tensile strength is good, and biological fitness is strong.At present known can synthetic cellulose bacterium have genus acetobacter (
Acetobacter), rhizobium (
Rhizobium), Sarcina (
Sarcina), false single pole Pseudomonas (
Pseudomonas), achromobacter (
Achrombacter), Alcaligenes (
Alcaligcncs), aerobacter (
Aerobacter), Azotobacter (
Azotobacter) and Agrobacterium (
Agrobacterium) wait 9 some kind that belongs in the bacteriums.Wherein, gluconate pyracetobacillus (
Gluconacetobacter xylinus) ability of synthetic cellulose is the strongest, has the potentiality that large scale fermentation is produced.As a kind of biodegradable natural cellulose, the bacteria cellulose range of application has related to industrial circles such as food, papermaking, medicine, weaving and environmental protection, but the high problem of production cost has limited its scale operation and widespread use.
Summary of the invention
The purpose of this invention is to provide a kind of is the method for raw material production bacteria cellulose with the oil, the coal chemical industry waste water that contain high concentration methanol, this method can reduce the production cost of bacteria cellulose, realizes resource utilization, the solution problem of environmental pollution of wastewater containing methanol simultaneously.
In order to achieve the above object, the invention provides a kind of is the method for raw material production bacteria cellulose with the waste water that contains high concentration methanol, comprise gluconate pyracetobacillus is inoculated in the nutrient solution and ferment, the gained bacteria cellulose obtains finished product through shaping, bleaching, drying, it is characterized in that, described nutrient solution with the oil that contains high concentration methanol, coal chemical industry waste water as carbon source and process water, its preparation method is: add nitrogenous source and inorganic salt in oil that contains high concentration methanol or coal chemical industry waste water, regulate pH value to 6.8～7.0.
The described oil of high concentration methanol, the waste water that coal chemical industry waste water produces when being mainly methanol rectification of containing, its temperature is 70～80 ℃.Containing main component in this waste water is long-chain compounds such as methyl alcohol, ethanol, higher alcohols and aldehydes and a small amount of paraffin, and wherein, the content of methyl alcohol is 0.5% ~ 5%(massfraction).
Need not in the preparation process of described nutrient solution through sterilising treatment.
The condition of described fermentation is: connect bacterium amount and be 5～10vol%, leavening temperature and be 30～35 ℃, fermentation time and be 70～120h, air demand and be 60～80%, shaking speed is 300～350r/min.
The present invention as thermal source, keeps leavening temperature by heat exchange with the oil that contains high concentration methanol, coal chemical industry waste water.
Also can add other carbon sources in the described nutrient solution, as glucose.Described nitrogenous source is preferably yeast powder, peptone or its composition.Described inorganic salt are preferably lime carbonate.
Utilization of the present invention contains the oil of high concentration methanol, the method that the coal chemical industry wastewater fermentation is produced bacteria cellulose, the high concentration methanol waste water that its invention core is to utilize oil, coal chemical industry is used as carbon source, process water and the thermal source of bacteria cellulose fermentative production, both handle high density, pyritous high-concentration industrial organic waste water, reduced the bacteria cellulose production cost simultaneously again.Technological innovation of the present invention embodies a concentrated reflection of " non-sterilization fermentation " and " 2 resource utilizations "." non-sterilization fermentation " refers to and adopts oil, the coal chemical industry waste water prepared culture contain methyl alcohol, can not need promptly can be used for fermentation through sterilization, this be because:
The bacteria cellulose leavening temperature is about 30 ℃, and the oil, the coal chemical industry waste water water temperature that contain methyl alcohol are generally 70～80 ℃, have avoided the pollution of most of mesophilic bacterias in the nutritive medium process for preparation;
Contain in the oil, coal chemical industry waste water of methyl alcohol and contain a certain amount of ethanol, not only can stimulate the growth and the cellulosic output of gluconate pyracetobacillus, can suppress varied bacteria growing during the fermentation simultaneously." 2 resource utilizations " refers to organism such as the methyl alcohol that contains in the oil that contains high concentration methanol, the coal chemical industry waste water, ethanol as the cheap carbon source resource utilization with contain oil, the coal chemical industry waste water used heat resource utilization of high concentration methanol.By the method for the invention, can effectively reduce the fermentation costs of bacteria cellulose, and reach the purpose of energy-saving and emission-reduction, realize cleaner production.
The present invention has the following advantages:
1, contain the oil of high concentration methanol, the organism such as methyl alcohol, ethanol and propyl alcohol in the coal chemical industry waste water are used as carbon source, add an amount of nitrogenous source and inorganic salt, regulate pH value to 6.8～7.0, the inoculation gluconate pyracetobacillus is produced bacteria cellulose, turn waste into wealth, realize that fermentation costs reduces and the wastewater organic pollutant reduction of discharging.
2, contain oil, the coal chemical industry waste water preparation nutrient solution of high concentration methanol, need not sterilising treatment and be directly used in fermentative production, reduce operation steps, realize that fermentation costs reduces.
3, oil, the coal chemical industry wastewater temperature that contains high concentration methanol is 70～80 ℃, keeps the fermentation unit temperature at 30～35 ℃ by heat exchange, improves the heat energy utilization rate, realizes that fermentation costs reduces, and avoids causing the environmental thermal pollution problem.
Embodiment
Below in conjunction with the indefiniteness specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
A, to adopt methanol content be the 0.5%(massfraction) petrochemical wastewater preparation nutritive medium, press mass volume ratio and add 1% yeast powder, 2% lime carbonate, to satisfy the nutritional requirement of microbial fermentation, regulate pH to 6.8 with hydrochloric acid, standby without sterilising treatment;
B, add gluconate pyracetobacillus dynamically ferment in above-mentioned nutrient solution, it is that 30 ℃, fermentation time are that 70h, air air demand are 60%, shaking speed is 300r/min for 5vol%, leavening temperature that control connects the bacterium amount, finishes fermentation;
C, the petrochemical wastewater temperature that contains methyl alcohol are 70～80 ℃, keep the fermentation unit temperature at 30 ℃ by heat exchange;
D, gained bacteria cellulose make finished product through shaping, bleaching, drying routinely.
Embodiment 2
A, to adopt methanol content be the 0.5%(massfraction) coal chemical industry waste water preparation nutritive medium, press the yeast powder of mass volume ratio adding 0.5% and 0.5% peptone, 1% lime carbonate, to satisfy the nutritional requirement of microbial fermentation, regulate pH to 6.8, standby without sterilising treatment;
B adds gluconate pyracetobacillus and dynamically ferments in above-mentioned nutrient solution, it is that 35 ℃, fermentation time are that 120h, air air demand are 80%, shaking speed is 350r/min for 10vol%, leavening temperature that control connects bacterium amount, finishes fermentation;
C, the coal chemical industry wastewater temperature that contains methyl alcohol are 70～80 ℃, keep the fermentation unit temperature at 35 ℃ by heat exchange;
D, gained bacteria cellulose make finished product through shaping, bleaching, drying routinely.
Embodiment 3
A, to adopt methanol content be the 4%(massfraction) petrochemical wastewater preparation nutritive medium, press the yeast powder of mass volume ratio adding 0.5% and 0.5% peptone, 2% lime carbonate, to satisfy the nutritional requirement of microbial fermentation, regulate pH to 7.0, standby without sterilising treatment;
B adds gluconate pyracetobacillus and dynamically ferments in above-mentioned nutrient solution, it is that 32 ℃, fermentation time are that 100h, air air demand are 70%, shaking speed is 320r/min for 7vol%, leavening temperature that control connects bacterium amount, finishes fermentation;
C, the petrochemical wastewater temperature that contains methyl alcohol are 70～80 ℃, keep the fermentation unit temperature at 32 ℃ by heat exchange;
D, gained bacteria cellulose make finished product through shaping, bleaching, drying routinely.
Embodiment 4
A, to adopt methanol content be the 4.5%(massfraction) coal chemical industry waste water preparation nutritive medium, press mass volume ratio and add 0.5% glucose, 0.5% yeast powder, 0.5% peptone, 2% lime carbonate, to satisfy the nutritional requirement of microbial fermentation, regulate pH to 7.0, standby without sterilising treatment;
B, add gluconate pyracetobacillus dynamically ferment in above-mentioned nutrient solution, it is that 33 ℃, fermentation time are that 85h, air air demand are 75%, shaking speed is 330r/min for 8vol%, leavening temperature that control connects the bacterium amount, finishes fermentation;
C, the coal chemical industry wastewater temperature that contains methyl alcohol are 70～80 ℃, keep the fermentation unit temperature at 33 ℃ by heat exchange;
D, gained bacteria cellulose make finished product through shaping, bleaching, drying routinely.
Claims (9)
1. one kind is the method for raw material production bacteria cellulose with the waste water that contains high concentration methanol, comprise gluconate pyracetobacillus is inoculated in the nutrient solution and ferment, the gained bacteria cellulose obtains finished product through shaping, bleaching, drying, it is characterized in that, described nutrient solution with the oil that contains high concentration methanol, coal chemical industry waste water as carbon source and process water, its preparation method is: add nitrogenous source and inorganic salt in oil that contains high concentration methanol or coal chemical industry waste water, regulate pH value to 6.8～7.0.
2. as claimed in claim 1 is the method for raw material production bacteria cellulose with the waste water that contains high concentration methanol, it is characterized in that, the mass concentration of methyl alcohol is 0.5% ~ 5% in the described oil that contains high concentration methanol, the coal chemical industry waste water.
3. as claimed in claim 1 is the method for raw material production bacteria cellulose with the waste water that contains high concentration methanol, it is characterized in that, need not in the preparation process of described nutrient solution through sterilising treatment.
4. as claimed in claim 1 is the method for raw material production bacteria cellulose with the waste water that contains high concentration methanol, it is characterized in that the condition of described fermentation is: connect bacterium amount and be 5～10vol%, leavening temperature and be 30～35 ℃, fermentation time and be 70～120h, air demand and be 60～80%, shaking speed is 300～350r/min.
5. as claimed in claim 1 is the method for raw material production bacteria cellulose with the waste water that contains high concentration methanol, it is characterized in that, as thermal source, keeps leavening temperature by heat exchange with the oil that contains high concentration methanol, coal chemical industry waste water.
6. as claimed in claim 1 is the method for raw material production bacteria cellulose with the waste water that contains high concentration methanol, it is characterized in that, also adds supplementary carbon source in the described nutrient solution.
7. as claimed in claim 6 is the method for raw material production bacteria cellulose with the waste water that contains high concentration methanol, it is characterized in that described supplementary carbon source is a glucose.
8. as claimed in claim 1 is the method for raw material production bacteria cellulose with the waste water that contains high concentration methanol, it is characterized in that described nitrogenous source is yeast powder, peptone or its composition.
9. as claimed in claim 1 is the method for raw material production bacteria cellulose with the waste water that contains high concentration methanol, it is characterized in that described inorganic salt are lime carbonate.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101307294A (en) * | 2008-06-02 | 2008-11-19 | 南京工业大学 | Pichia yeast for rapid utilizing industrial methanol and uses thereof |
| CN101487033A (en) * | 2009-02-23 | 2009-07-22 | 天津科技大学 | Preparation of bacteria cellulose special-shaped product by microbial fermentation direct biosynthesis |
| CN101608167A (en) * | 2009-01-20 | 2009-12-23 | 海南椰国食品有限公司 | The method of gluconacetobacter oboediens strain and seed selection thereof and production bacteria cellulose |
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2011
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101307294A (en) * | 2008-06-02 | 2008-11-19 | 南京工业大学 | Pichia yeast for rapid utilizing industrial methanol and uses thereof |
| CN101608167A (en) * | 2009-01-20 | 2009-12-23 | 海南椰国食品有限公司 | The method of gluconacetobacter oboediens strain and seed selection thereof and production bacteria cellulose |
| CN101487033A (en) * | 2009-02-23 | 2009-07-22 | 天津科技大学 | Preparation of bacteria cellulose special-shaped product by microbial fermentation direct biosynthesis |
Non-Patent Citations (5)
| Title |
|---|
| 关晓辉等: "细菌纤维素发酵条件的优化及结构分析", 《中国酿造》 * |
| 张凤清等: "玉米浆做Acetobacter xylinum培养基生产纤维素的发酵条件优化", 《食品工业科技》 * |
| 邵伟等: "巴氏醋酸杆菌发酵处理甲醇废水合成细菌纤维素的研究", 《化工环保》 * |
| 邵伟等: "醋酸菌对甲醇的净化及细菌纤维素合成研究", 《生物技术》 * |
| 马霞等: "有机酸对木醋杆菌合成细菌纤维素的影响规律", 《纤维素科学与技术》 * |
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Application publication date: 20110817 |