CN102154386A - Method for preparing short-chain fatty acid by fermenting rinsing liquor of hemicellulose from steam-exploded straws - Google Patents
Method for preparing short-chain fatty acid by fermenting rinsing liquor of hemicellulose from steam-exploded straws Download PDFInfo
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- CN102154386A CN102154386A CN2011100341958A CN201110034195A CN102154386A CN 102154386 A CN102154386 A CN 102154386A CN 2011100341958 A CN2011100341958 A CN 2011100341958A CN 201110034195 A CN201110034195 A CN 201110034195A CN 102154386 A CN102154386 A CN 102154386A
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- fatty acid
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Abstract
The invention relates to a method for preparing short-chain fatty acid by fermenting rinsing liquor of hemicellulose from steam-exploded straws. The method comprises the following steps: soaking the straws after being subjected to steam explosion treating, filtrating to obtain rinsing liquor; leading into anaerobic acidified sludge by 10-30 percent, regulating the pH to be neutral, introducing N2 into a reactor to cause an anaerobic environment, fermenting for 3-7 days on the condition of 35-40 DEG C, and discharging the produced gas by a guide pipe during fermentation period in time. According to the method, the problem of utilizing the hemicellulose degraded product in the stream-exploded straws is solved; and the obtained short-chain fatty acid can be further used as subsequent fermentation substrate, thus improving the utilization rate of the straws, and providing cheap carbon source for the production of subsequent products.
Description
Technical field
The present invention relates to a kind of preparation method of short chain fatty acid, particularly a kind of method of using steam puffed stalk hemicellulose water lotion fermentative preparation short chain fatty acid.
Background technology
Along with the shortage of fossil energy and the enhancing gradually of people's Environmental awareness, the biomass resource of exploitation cleaning energetically, recyclability is rooted in the hearts of the people.China's straw biomass aboundresources is produced per year more than 700,000,000 tons, is used as fuel at present mostly or is directly burnt in the field, has polluted environment, has destroyed the eubiosis, simultaneously, because straw burning heat energy utilization rate is low, also is great waste to resource.Therefore, develop and utilize biotechnology that its decomposition and inversion is become energy product and various Chemicals, realize its higher value application, be of great immediate significance for solving environmental pollution, energy shortage, crisis in food.
But the straw cell wall structure is very tight, its three big component, twine each other between Mierocrystalline cellulose, hemicellulose and the xylogen, closely crosslinked together, because chemical structure is with qualitative different, cause each component utilization ratio very low, need through suitable pretreatment process, destruction or change part-structure are to realize the separation and the utilization of component.The quick-fried The Application of Technology of vapour, the utilization ratio of stalk is improved greatly, mechanical effect by high temperature steaming effect and instant decompression generation, hemicellulose is degraded into the oligopolymer of solubility, be mainly wood sugar and xylo-oligosaccharide, simultaneously the Mierocrystalline cellulose position is fully exposed, improved enzymatic hydrolyzation effectively.
At present, people pay close attention to the situation that is more conducive to the cellulosic component enzymolysis after the quick-fried processing of vapour more, have ignored the utilization that is degraded into the hemicellulose of solubility oligopolymer mostly.And the quick-fried process of vapour can produce some inhibitions to follow-up microbial fermentation, as furfural etc., needs sometimes and will utilize after the stalk washing of the quick-fried back of vapour again, and water lotion directly is discharged into and goes in the environment, not only brings environmental pollution, and has caused the waste of resource.
Short chain fatty acid such as acetate, propionic acid, butanic acid etc. not only can be used as the raw material production high value-added product of fermentation industry, also can be used as the synthetic other products of industrial chemicals.It is synthetic that the production of China's short chain fatty acid at present mostly is chemical method, and its output also can not satisfy the demands far away, needs a large amount of imports every year.
At above situation, the present invention utilizes abundant and cheap stalk resource, quick-fried with vapour as the pretreated means of stalk, hemicellulose in the stalk is degraded into water miscible oligopolymer, extract through soaking, reclaim hemicellulose degradation product in the quick-fried water lotion of vapour, be that utilization of carbon source anaerobic acidification sludge fermentation prepares short chain fatty acid with it again, so not only make the degradation product of half fibre fractionation after vapour is quick-fried in the stalk be fully used, and the production cost of fermentation short chain fatty acid is reduced greatly, thereby provide new thinking for the higher value application of stalk.
Summary of the invention
The object of the present invention is to provide a kind of method of steam puffed stalk hemicellulose water lotion fermentative preparation short chain fatty acid, promptly utilize the quick-fried technology of vapour that hemicellulose component degradation in the stalk is become oligopolymer, extract through soaking, obtain a large amount of wood sugars, xylo-oligosaccharide, and, prepare short chain fatty acid by the anaerobic acidification sludge fermentation as substrate.The invention solves the problem of utilizing of hemicellulose in the steam puffed stalk, simultaneously, the short chain fatty acid that makes can be used as the good cheap carbon source of multiple microorganism, production various energy resources and Chemicals, thereby both improved the comprehensive utilization ratio of stalk, walk out the road of a stalk higher value application again, had good benefits in environment and economic benefit.
Technical scheme of the present invention is as follows:
The method of steam puffed stalk hemicellulose water lotion fermentative preparation short chain fatty acid provided by the invention, its step is as follows:
1) preparation of steam puffed stalk hemicellulose water lotion
It is long that stalk is cut into 3-5cm, soaks 30-60min, stalk with adding the quality ratio be 1: 0.5-1: 1, stalk is handled 5-20min under pressure 0.8-2.5MPa condition, discharge explosion then rapidly, promptly get steam puffed stalk; Add its weight in wet base 1-4 warm water soaking 30-60min doubly in steam puffed stalk, it is 40-70 ℃ that institute adds warm water temperature, and the extruding separation obtains steam puffed stalk hemicellulose water lotion, contains water miscible oligopolymer in this water lotion, mainly is wood sugar and xylo-oligosaccharide.
2) steam puffed stalk hemicellulose water lotion fermentative preparation short chain fatty acid
In the steam puffed stalk hemicellulose water lotion that step 1) obtains, press the 10%-30% volume ratio and insert anaerobic acidification mud as seed liquor, then pH is transferred to neutrality, in reactor, feed N again
2Cause anaerobic environment, at 30-40 ℃ of condition bottom fermentation 3-7d, aerogenesis is in time discharged through conduit between yeast phase;
Described anaerobic acidification mud is anaerobically digested sludge gained after heating (95-105 ℃ of heating 15-120min) is handled;
Described stalk is one or more in wheat, corn or rice straw and other straw.A kind of steam puffed stalk hemicellulose water lotion of the present invention prepares the method for short chain fatty acid, has the following advantages: at first, the invention solves the problem of utilizing of steam puffed stalk hemicellulose, improved the comprehensive utilization ratio of stalk; Secondly,, thereby reduced production cost effectively, satisfied the market requirement for follow-up microbial fermentation provides cheap carbon source-short chain fatty acid.
Embodiment
The invention is further illustrated by the following examples.
Embodiment 1
Maize straw is cut into 3-5cm, gets the maize straw 200g that cuts, add the 200ml tap water and soak 60min, in the steam-explosion jar of then it being packed into, dimension is pressed among the 5min and is discharged rapidly from steam-explosion jar under pressure 2.0MPa, promptly gets steam puffed stalk; Add the 800mL tap water then in steam puffed stalk, soak 30min down at 70 ℃, the extruding separation obtains steam puffed stalk hemicellulose water lotion; This water lotion branch is installed in several 500ml triangular flasks, insert anaerobic acidification mud, pH is transferred to neutrality, carry out anaerobically fermenting by 10% volume ratio, 35 ℃ of leavening temperatures, fermentation time 5d makes short chain fatty acid.
Used anaerobic acidification mud is taken from sewage work's anaerobically digested sludge in this example, makes through 100 ℃ of heat treated 30min.
Embodiment 2
Wheat stalk is cut into 3-5cm, gets 200g, add the 150ml tap water and soak 30min, in the steam-explosion jar of packing into, dimension is pressed 6min under pressure 1.5MPa, discharges rapidly from steam-explosion jar and explosion promptly gets steam puffed stalk; Add the 800mL tap water then in steam puffed stalk, soak 30min down at 70 ℃, the extruding separation obtains steam puffed stalk hemicellulose water lotion; This water lotion branch is installed in several 500ml triangular flasks, insert anaerobic acidification mud, pH is transferred to neutrality, carry out anaerobically fermenting by 10% volume, 35 ℃ of leavening temperatures, fermentation time 5d makes short chain fatty acid.
Used anaerobic acidification mud is taken from sewage work's anaerobically digested sludge in this example, makes through 105 ℃ of heat treated 60min.
Embodiment 3
Broomcorn straw is cut into 3-5cm, gets 200g, add the 200ml tap water and soak 60min, in the steam-explosion jar of packing into then, dimension is pressed 10min under pressure 1.5MPa, discharges rapidly from steam-explosion jar and explosion promptly gets steam puffed stalk; In steam puffed stalk, add the 500mL tap water then, soak 60min down, separate promptly getting steam puffed stalk hemicellulose water lotion through extruding at 70 ℃; Then this water lotion branch is installed in several 500ml triangular flasks, insert anaerobic acidification mud, pH is transferred to neutrality, carry out anaerobically fermenting by 20% volume, 35 ℃ of leavening temperatures, fermentation time 3d makes short chain fatty acid.Used anaerobic acidification mud is taken from sewage work's anaerobically digested sludge in this example, makes through 95 ℃ of heat treated 100min.
Claims (7)
1. the method for a steam puffed stalk hemicellulose water lotion fermentative preparation short chain fatty acid is characterized in that concrete steps are as follows:
1) pushes separation after the stalk after quick-fried is soaked in water with vapour, obtain steam puffed stalk hemicellulose water lotion;
2) the steam puffed stalk hemicellulose water lotion that obtains by step 1) inserts 10%-30% anaerobic acidification mud, regulates pH to neutral, carries out anaerobically fermenting after stirring, the preparation short chain fatty acid.
2. method according to claim 1 is characterized in that, described soaking conditions is that amount of water is 1-4 a times of the quick-fried back of vapour stalk weight in wet base, temperature 40-70 ℃, and time 30-60min.
3. method according to claim 1 is characterized in that, described stalk is one or more in wheat, corn or rice straw and other straw.
4. method according to claim 1 is characterized in that, described anaerobic acidification mud is through heat treated anaerobically digested sludge.
5. method according to claim 4 is characterized in that, described heat-treat condition is 95-105 ℃ of heating 15-120min.
6. method according to claim 1 is characterized in that, the time of described fermentative preparation short chain fatty acid is 3-7 days.
7. method according to claim 1 is characterized in that, described leavening temperature is 30-40 ℃.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011100341958A CN102154386A (en) | 2011-02-01 | 2011-02-01 | Method for preparing short-chain fatty acid by fermenting rinsing liquor of hemicellulose from steam-exploded straws |
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| CN2011100341958A CN102154386A (en) | 2011-02-01 | 2011-02-01 | Method for preparing short-chain fatty acid by fermenting rinsing liquor of hemicellulose from steam-exploded straws |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103305558A (en) * | 2013-05-09 | 2013-09-18 | 同济大学 | Method for producing short-chain fatty acid by promoting combined anaerobic fermentation of excess sludge and ryegrass through thermal pretreatment |
| CN103304042A (en) * | 2013-06-20 | 2013-09-18 | 同济大学 | Method for preparing rapid external carbon source through cassava ethanol wastewater |
| CN105063109A (en) * | 2015-09-02 | 2015-11-18 | 钟华 | Method for obtaining short-chain fatty acids by vegetable protein microbial fermentation |
| CN105861576A (en) * | 2016-04-19 | 2016-08-17 | 湖南大学 | Method for producing volatile fatty acid (VFA) from mushroom cultured residue through alkaline fermentation |
| CN112811715A (en) * | 2020-12-28 | 2021-05-18 | 吉林农业大学 | Sewage treatment system utilizing primary sludge fermentation |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101148630A (en) * | 2006-09-19 | 2008-03-26 | 中国科学院过程工程研究所 | Method for preparing microorganism grease by fermenting steam-exploded straw hemicellulose hydrolysate |
| CN101314783A (en) * | 2008-06-02 | 2008-12-03 | 江南大学 | Method for preparing volatile fatty acid with high solid concentration organic castoff heat-alkali preprocessing post anaerobic fermentation |
| CN101475964A (en) * | 2009-01-16 | 2009-07-08 | 南京大学 | Novel wood fiber raw material anaerobic fermentation process |
-
2011
- 2011-02-01 CN CN2011100341958A patent/CN102154386A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101148630A (en) * | 2006-09-19 | 2008-03-26 | 中国科学院过程工程研究所 | Method for preparing microorganism grease by fermenting steam-exploded straw hemicellulose hydrolysate |
| CN101314783A (en) * | 2008-06-02 | 2008-12-03 | 江南大学 | Method for preparing volatile fatty acid with high solid concentration organic castoff heat-alkali preprocessing post anaerobic fermentation |
| CN101475964A (en) * | 2009-01-16 | 2009-07-08 | 南京大学 | Novel wood fiber raw material anaerobic fermentation process |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103305558A (en) * | 2013-05-09 | 2013-09-18 | 同济大学 | Method for producing short-chain fatty acid by promoting combined anaerobic fermentation of excess sludge and ryegrass through thermal pretreatment |
| CN103305558B (en) * | 2013-05-09 | 2014-11-05 | 同济大学 | Method for producing short-chain fatty acid by promoting combined anaerobic fermentation of excess sludge and ryegrass through thermal pretreatment |
| CN103304042A (en) * | 2013-06-20 | 2013-09-18 | 同济大学 | Method for preparing rapid external carbon source through cassava ethanol wastewater |
| CN105063109A (en) * | 2015-09-02 | 2015-11-18 | 钟华 | Method for obtaining short-chain fatty acids by vegetable protein microbial fermentation |
| CN105861576A (en) * | 2016-04-19 | 2016-08-17 | 湖南大学 | Method for producing volatile fatty acid (VFA) from mushroom cultured residue through alkaline fermentation |
| CN112811715A (en) * | 2020-12-28 | 2021-05-18 | 吉林农业大学 | Sewage treatment system utilizing primary sludge fermentation |
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Application publication date: 20110817 |