CN102154382B - Method for producing cellulosic ethanol by using 1308 Saccharomyces cerevisiae - Google Patents
Method for producing cellulosic ethanol by using 1308 Saccharomyces cerevisiae Download PDFInfo
- Publication number
- CN102154382B CN102154382B CN2011100044646A CN201110004464A CN102154382B CN 102154382 B CN102154382 B CN 102154382B CN 2011100044646 A CN2011100044646 A CN 2011100044646A CN 201110004464 A CN201110004464 A CN 201110004464A CN 102154382 B CN102154382 B CN 102154382B
- Authority
- CN
- China
- Prior art keywords
- ethanol
- saccharomyces cerevisiae
- cultivate
- yeast
- domestication
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a method for producing cellulosic ethanol by using 1308 Saccharomyces cerevisiae, which is mainly characterized in that through three times of tolerance domestication in hydrolysis solution of straw cellulose, the 1308 Saccharomyces cerevisiae strain can tolerate the influences of toxic and harmful materials such as acetic acid and furfural in the hydrolysis solution of straw cellulose and adapt to the components of the hydrolysis solution, has a characteristic of efficiently converting glucose into ethanol and high fermentation inhibiting material resistance, and therefore can convert the glucose in the hydrolysis solution into ethanol efficiently. When the method for producing cellulosic ethanol is implemented, the rate of conversion of glucose into ethanol reaches a theoretical conversion rate of 80 percent, and a reliable basic condition is provided for the large-scale production of straw ethanol and reducing production cost.
Description
Technical field
The present invention relates to yeast saccharomyces cerevisiae (Saccharomyces cerevisiae); Deposit number is the new purposes of CGMCC 1308 (Nanyang mixed yeast); Utilizing stalk to produce in the fuel ethanol production specifically, the fermentation of cellulose hydrolysis product is the application in the ethanol.
Background technology
Scarcity day by day along with fossil oil relies on fossil energy to be difficult to realize the Sustainable development of economic society and environment merely.Renewable Energy Development has become alleviates energy supply and demand contradiction, reduces environmental pollution, the important channel of increasing farmers' income.Produce ethanol with non-grain material-stalk, be not only the needs that guarantee national energy security, grain security, and be the protection environment, improve the effective means of farmers' income.
Stalk alcoholic acid operational path is: 1. stalk cuts off: stalk is delivered to chaffcutter through rotary conveyor and carries out the segment processing after knot screen carries out removal of impurities; 2. stalk pre-treatment: the stalk after the segment is sent into pre-treatment workshop section, carries out pre-treatment, and xylogen is separated with Mierocrystalline cellulose; 3. enzymatic saccharification: pretreated stalk gets into enzymatic vessel, adopts prozyme to degrade, and obtains fermentable sugars; 4. fermentation: enzymatic saccharification finishes, and enzymolysis solution is transported to fermentor tank inoculation yeast saccharomyces cerevisiae ferments, and is converted into ethanol to fermentable sugars; 5. distillation: the mash entering distillation process of fermenting-ripening distills and makes ethanol.
The staple of stalk fibre cellulose hydrolysate using: the mixing sugar concentration 10% of glucose, wood sugar, cellobiose, these materials can be converted into ethanol.In addition owing to receive the influence of stalk pretreatment technology; Also contain the material that inhibition yeast such as acetate, furfural and phenolic cpd are grown or influenced its leavening property in the hydrolyzed solution; The concentration of these materials is respectively: acetate 5000～10000ppm, furfural 1000～2000ppm, and contain phenols and compound fragrant hydrocarbon.These materials are having a strong impact on yeast and are being converted into the alcoholic acid ability to monose capable of using; Therefore; The bacterial strain that is used to ferment is the alcoholic acid characteristics except that having efficient transforming glucose, also should possess stronger anti-fermentation inhibitor ability, could improve the sugar alcohol transformation efficiency of stalk cellulose hydrolysis monose.
Seek or the domestication yeast strain, the stalk fibre cellulose hydrolysate using is fermented, efficient metabolizable glucose is produced the alcoholic acid cost to reducing stalk cellulose, carries out large-scale industrial production and is of great importance.
Summary of the invention
Yeast saccharomyces cerevisiae 1308 (Nanyang mixed yeast) primary characteristic is to be main with starchy material hydrolysis monose; Normal circumstances bottom fermentation performance is: sugar alcohol transformation efficiency 43～46%; About remaining reducing sugar 0.5g/100ml, this yeast is the fermented bacterium that generally uses in the starchy material ethanol fermentation technology.But with the starchiness is in the saccharification liquid of raw material, do not have acetate, furfural, etc. suppress the material of its growth and leavening property.
The object of the invention is to provide a kind of application of yeast saccharomyces cerevisiae 1308 in the fermentation of stalk fibre cellulose hydrolysate using of using,
The present invention provides a kind of stalk cellulose hydrolysis to prepare the method for cellulose ethanol for this reason, and this method may further comprise the steps:
Step 1 is tamed yeast saccharomyces cerevisiae 1308;
Step 2 is inoculated into cellulosic hydrolysate with the yeast saccharomyces cerevisiae after the domestication and cultivates, and obtains cellulose ethanol.
Yeast saccharomyces cerevisiae 1308 described in the step 1 of the present invention; Be to be deposited in Chinese common micro-organisms culture presevation administrative center; Yard 1, BeiChen xi Road, Chaoyang District, Beijing City institute of microbiology of the Chinese Academy of Sciences is numbered the yeast saccharomyces cerevisiae of CGMCC 1308 (Nanyang mixed yeast), belongs to prior art.
The present invention utilizes this zymic advantage, obtains better bacterial classification through further taming.
Acclimation method in the step 1 of the present invention is following:
Acclimation method:
Domestication for the first time: the yeast saccharomyces cerevisiae 1308 that picking grows fine inserts in the 1308 yeast first order seed substratum; Cultivate 8～12h under 28～30 ℃, the condition of 150rpm; Make first class inoculum, insert to expand in the fermention medium by 10% inoculum size then and join, temperature is controlled at 28～30 ℃; Ventilating ratio 0.05～0.1vvm cultivates 12～16h.Cultivate sophisticated seed and insert in 20% cellulosic hydrolysate, 28～30 ℃, the condition bottom fermentation of 150rpm 2～3 days with 10% inoculum size;
Domestication for the second time: the yeast that the first time that picking grows fine, domestication obtained inserts in the 1308 yeast first order seed substratum; Cultivate 8～12h under 28～30 ℃, the condition of 150rpm; Make first class inoculum, insert to expand in the fermention medium by 10% inoculum size then and join, temperature is controlled at 28～30 ℃; Ventilating ratio 0.05～0.1vvm cultivates 12～16h.Cultivate sophisticated seed and insert in 50% cellulosic hydrolysate, 28～30 ℃, the condition bottom fermentation of 150rpm 2～3 days with 10% inoculum size;
Domestication for the third time: the yeast that the second time that picking grows fine, domestication obtained inserts in the 1308 yeast first order seed substratum; Cultivate 8～12h under 28～30 ℃, the condition of 150rpm; Make first class inoculum, insert to expand in the fermention medium by 10% inoculum size then and join, temperature is controlled at 28～30 ℃; Ventilating ratio 0.05～0.1vvm cultivates 12～16h.Cultivate sophisticated seed and insert in 100% cellulosic hydrolysate, 28～30 ℃, the condition bottom fermentation of 150rpm 2～3 days with 10% inoculum size.
Alcoholic acid preparation in the step 2 of the present invention:
The yeast that the domestication for the third time that picking grows fine obtains inserts in the 1308 yeast first order seed substratum; Cultivate 8～12h under 28～30 ℃, the condition of 150rpm; Make first class inoculum, insert to expand in the fermention medium by 10% inoculum size then and join, temperature is controlled at 28～30 ℃; Ventilating ratio 0.05～0.1vvm cultivates 12～16h.Cultivate sophisticated seed with in the 10% inoculum size incoming fiber cellulose hydrolysate, fermented 2～3 days.
Above-described substratum is:
(1) 1308 yeast first order seed substratum: 12
°The Bx wort.
(2) fermention medium: 1% steeping water, all the other are cellulose hydrolysis product stoste, pH value 5.0.
After three domestications through the tolerance of 1308 bacterial strains in the stalk fibre cellulose hydrolysate, this bacterial strain can adapt to the composition in the hydrolyzed solution, and fermentation ends glucose reaches 80% of theoretical yield to ethanol conversion.Bacterial strain after the present invention tames through three times is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center; Be numbered CGMCC № 4479, preservation date 2010.12.16, classification name: yeast saccharomyces cerevisiae; The TG-II strain, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.Therefore the present invention also comprises the application of Wine brewing yeast strain in ethanol preparation that is numbered CGMCC № 4479.Said application comprises that the Wine brewing yeast strain of CGMCC № 4479 is inoculated into cellulosic hydrolysate and cultivates, and obtains the step of cellulose ethanol.
Embodiment
Through the practical implementation method essence of the present invention is described below.
Implementation method 1:
The technical scheme that the present invention takes the tolerance domestication of 1308 bacterial strains:
1. bacterial classification is selected: domestication is adopted with bacterial strain and is deposited in Chinese common micro-organisms culture presevation administrative center, is numbered the yeast saccharomyces cerevisiae of CGMCC 1308 (Nanyang mixed yeast).
2. substratum: dull and stereotypedly cultivate and the slant preservation substratum 12
°The Bx wort, 2% agar powder, 121 ℃, 30min sterilising treatment behind the mixing.Seed culture medium, 12
°The Bx wort, 121 ℃, 30min sterilising treatment.Fermention medium, glucose 10%, peptone 2%, yeast extract paste 1% adds the water mixing after 121 ℃, 30min sterilising treatment.
(3) add acetate domestication test:
The domestication scheme: in plate culture medium, add the acetate of 2000ppm, cultivate and finish to select the single colony inoculation inclined-plane that grows fine, cultivate 3～5d for 30 ℃, 4 ℃ of preservations of refrigerator are subsequent use.Slant strains after the activation is inserted in the seed culture medium of the acetate that adds 2000ppm; Cultivate 8～12h under 28～30 ℃, the condition of 150rpm, make fermented bacterium, insert by 10% inoculum size in the fermention medium of the same 2000ppm of adding acetate; Temperature is controlled at 30～32 ℃; Fermented 2～3 days, and detected ethanol content, calculate the sugar alcohol transformation efficiency.
Fermentation ends choose grow fine, acetate domestication experiment that bacterial strain that the sugar alcohol transformation efficiency is high carries out next high density, the rest may be inferred, the result is following in domestication.
(4) add furfural domestication test:
The domestication scheme: in plate culture medium, add the furfural of 400ppm, cultivate and finish to select the single colony inoculation inclined-plane that grows fine, cultivate 3～5d for 30 ℃, 4 ℃ of preservations of refrigerator are subsequent use.Slant strains after the activation is inserted in the seed culture medium that adds the 400ppm furfural; Cultivate 8～12h under 28～30 ℃, the condition of 150rpm, make fermented bacterium, insert by 10% inoculum size in the fermention medium of the same 400ppm of adding furfural; Temperature is controlled at 30～32 ℃; Fermented 2～3 days, and detected ethanol content, calculate the sugar alcohol transformation efficiency.
Fermentation ends choose grow fine, furfural domestication experiment that bacterial strain that the sugar alcohol transformation efficiency is high carries out next high density, the rest may be inferred, the result is following in domestication.
(4) add acetate, furfural mixture domestication test:
The domestication scheme: in plate culture medium, add the furfural of 400ppm and the acetate of 2000ppm, cultivate and finish to select the single colony inoculation inclined-plane that grows fine, cultivate 3～5d for 30 ℃, 4 ℃ of preservations of refrigerator are subsequent use.Slant strains after the activation is inserted in the substratum that adds 400ppm furfural and 2000ppm acetate; Cultivate 8～12h under 28～30 ℃, the condition of 150rpm, make fermented bacterium, insert by 10% inoculum size in the acetic acid fermentation substratum of furfural and 2000ppm of the same 400ppm of adding; Temperature is controlled at 30～32 ℃; Fermented 2～3 days, and detected ethanol content, calculate the sugar alcohol transformation efficiency.
Fermentation ends choose grow fine, furfural domestication experiment that bacterial strain that the sugar alcohol transformation efficiency is high carries out next high density, the rest may be inferred, the result is following in domestication.
Can find out that by table 1～3 the Nanyang mixed yeast 1308 after the domestication has efficient transforming glucose and generates the alcoholic acid ability, have simultaneously can tolerate fermentable suppressor factor acetate 6000～8000ppm furfural 1200～1600ppm ability.
Picking adds the bacterium colony that grows fine on the plate culture medium of 8000ppm furfural and 1200ppm and carries out slant preservation and next step domestication experiment.
The present invention improves the tolerance performance of bacterial classification to the stalk fibre cellulose hydrolysate further through the domestication to bacterial classification, is acclimation method below.
Implementation method 2:
The technical scheme that the present invention takes the tolerance standardization of stalk fibre cellulose hydrolysate 1308 bacterial strains:
(1) substratum: 1308 yeast starter substratum, 12
°The Bx wort.
(2) fermention medium: 1% steeping water, all the other are cellulose hydrolysis product stoste, pH value 5.0.
(3) domestication scheme: the slant strains that picking grows fine inserts in the first order seed enlarged culturing base; Cultivate 8～12h under 28～30 ℃, the condition of 150rpm, make first class inoculum, insert to expand in the seed culture medium by 10% inoculum size then and join; Substratum is the same; Temperature is controlled at 28～30 ℃, and ventilating ratio 0.05～0.1vvm cultivates 12～16h.Cultivate sophisticated seed with in the 10% inoculum size incoming fiber cellulose hydrolysate, 28～30 ℃, the condition bottom fermentation of 150rpm 2～3 days detect ethanol content, calculate the sugar alcohol transformation efficiency.
Can find out through implementation method 2; After the tolerance domestication of 1308 bacterial strains in the stalk fibre cellulose hydrolysate; This bacterial strain can adapt to the composition in the hydrolyzed solution; Fermentation ends glucose reaches 80% of theoretical yield to ethanol conversion, and the be hydrolyzed tolerance of liquid of the Wine brewing yeast strain of the CGMCC4479 that therefore considers domestication is for the third time obtained is amplified production test.
Implementation method 3:
1308 bacterial strains amplify scheme in the production of 20 tons of stalk fibre cellulose hydrolysates:
(1) substratum: 1308 yeast first order seed substratum, 12
°The Bx wort;
(2) fermention medium: 1% steeping water, all the other are cellulose hydrolysis product stoste, pH value 5.0;
(3) culture condition: the Wine brewing yeast strain slant strains of the CGMCC4479 that the domestication for the third time that picking grows fine obtains inserts in the first order seed enlarged culturing base; Cultivate 8～12h under 28～30 ℃, the condition of 150rpm, make first class inoculum, insert to expand in the secondary seed medium by 10% inoculum size then and join; Substratum is the same; Temperature is controlled at 28～30 ℃, and ventilating ratio 0.05～0.1vvm cultivates 12～16h.Cultivate sophisticated seed with in the 10% inoculum size incoming fiber cellulose hydrolysate, fermented 2～3 days, detect reducing sugar and ethanol content.
Can be found out that by above data through domestication, yeast saccharomyces cerevisiae 1308 can tolerate the influence of hazardous and noxious substances in the stalk fibre cellulose hydrolysate fully, after being applied to produce, glucose reaches 80% to ethanol conversion.
Content of the present invention is to provide a strain can tolerate hazardous and noxious substances influences such as acetate, furfural in the stalk fibre cellulose hydrolysate; And can efficiently be converted into alcoholic acid Wine brewing yeast strain 1308 to glucose in the hydrolyzed solution, the present invention implements the back and has great importance for the large-scale production of stalk alcoholic acid, aspect such as reduce production costs.
Claims (4)
1. Wine brewing yeast strain through standardization is deposited in Chinese common micro-organisms culture presevation administrative center, is numbered CGMCC4479, and preservation date 2010.12.16 classifies and names: yeast saccharomyces cerevisiae, TG-II strain.
2. the application of the Wine brewing yeast strain of claim 2 in ethanol preparation.
3. the application of claim 3 is characterized in that, Wine brewing yeast strain is inoculated into cellulosic hydrolysate and cultivates, and obtains cellulose ethanol.
4. the application of claim 3 is characterized in that, step is following:
The Wine brewing yeast strain that picking grows fine inserts in the yeast saccharomyces cerevisiae 1308 first order seed substratum; Cultivate 8～12h under 28～30 ℃, the condition of 150rpm; Make first class inoculum, insert in the fermention medium by 10% inoculum size then and cultivate, temperature is controlled at 28～30 ℃; Ventilating ratio 0.05～0.1vvm cultivates 12～16h; Cultivate sophisticated seed with in the 10% inoculum size incoming fiber cellulose hydrolysate, fermented 2～3 days;
Wherein, said yeast saccharomyces cerevisiae 1308 first order seed substratum are 12
°The Bx wort;
Said fermention medium is 1% steeping water, and all the other are cellulose hydrolysis product stoste, the substratum of pH value 5.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100044646A CN102154382B (en) | 2011-01-11 | 2011-01-11 | Method for producing cellulosic ethanol by using 1308 Saccharomyces cerevisiae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100044646A CN102154382B (en) | 2011-01-11 | 2011-01-11 | Method for producing cellulosic ethanol by using 1308 Saccharomyces cerevisiae |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102154382A CN102154382A (en) | 2011-08-17 |
| CN102154382B true CN102154382B (en) | 2012-08-01 |
Family
ID=44436054
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011100044646A Expired - Fee Related CN102154382B (en) | 2011-01-11 | 2011-01-11 | Method for producing cellulosic ethanol by using 1308 Saccharomyces cerevisiae |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102154382B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102296034B (en) * | 2011-08-18 | 2013-07-31 | 天津大学 | Method for obtaining yeast strain with tolerance on various inhibitors |
| CN102586127B (en) * | 2012-03-09 | 2013-04-03 | 江南大学 | Saccharomyces cerevisiae deleted bacterial strain and application thereof in furfural resistant aspect |
| CN104561127A (en) * | 2014-12-30 | 2015-04-29 | 松原光禾能源有限公司 | Comprehensive utilization method of agricultural straw |
| CN105985915A (en) * | 2015-02-09 | 2016-10-05 | 中国农业科学院作物科学研究所 | Saccharomyces cerevisiae recombinant strain for regulating expression activity of GRE3 genes and application of recombinant strain |
| CN107779479B (en) * | 2016-08-31 | 2021-03-02 | 安琪酵母股份有限公司 | Saccharomyces cerevisiae composition for producing ethanol and method for producing ethanol by using saccharomyces cerevisiae composition |
| CN109251868B (en) * | 2017-07-13 | 2022-03-11 | 吉林中粮生化有限公司 | Saccharomyces cerevisiae and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101434913A (en) * | 2008-12-12 | 2009-05-20 | 华东理工大学 | Wine brewing yeast strain and method for producing ethanol by efficient stalk fermentation |
| CN101555494A (en) * | 2008-04-09 | 2009-10-14 | 北京化工大学 | Method for preparing fuel of ethanol from immobilized mixed strain fermented cellulosic hydrolysate |
| CN101928675A (en) * | 2010-08-11 | 2010-12-29 | 山东大学 | Vanillin-tolerant saccharomyces cerevisiae |
-
2011
- 2011-01-11 CN CN2011100044646A patent/CN102154382B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101555494A (en) * | 2008-04-09 | 2009-10-14 | 北京化工大学 | Method for preparing fuel of ethanol from immobilized mixed strain fermented cellulosic hydrolysate |
| CN101434913A (en) * | 2008-12-12 | 2009-05-20 | 华东理工大学 | Wine brewing yeast strain and method for producing ethanol by efficient stalk fermentation |
| CN101928675A (en) * | 2010-08-11 | 2010-12-29 | 山东大学 | Vanillin-tolerant saccharomyces cerevisiae |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102154382A (en) | 2011-08-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101434913B (en) | Wine brewing yeast strain and method for producing ethanol by efficient stalk fermentation | |
| CN102154382B (en) | Method for producing cellulosic ethanol by using 1308 Saccharomyces cerevisiae | |
| CN102321722B (en) | Method for producing ethanol fuel from kitchen waste | |
| CN1944658B (en) | Method for producing cellulose alcohol using corncob processing leftover by fermenting | |
| CN109706089A (en) | A kind of xylose-fermenting strains being resistant to mortifier and its construction method and application | |
| CN102296034B (en) | Method for obtaining yeast strain with tolerance on various inhibitors | |
| CN110358690A (en) | A kind of S. cervisiae of resistance to mortifier and its selection and application | |
| CN101638673A (en) | Method for manufacturing alcohol by utilizing fermentation of plant straws | |
| CN103820347B (en) | One strain has an industrial strain of S.cerevisiae strain of Acetate tolerance | |
| CN103374532A (en) | High-temperature-resistant saccharomycete for producing ethanol and application thereof | |
| CN104073525A (en) | Method for producing ethanol by virtue of simultaneous saccharification and co-fermentation | |
| CN102286600B (en) | Method for simultaneously producing ethanol and hydrogen by using cassava residue through fermentation | |
| CN101709309B (en) | Method for combined fermentation of ethanol and xylitol | |
| CN111118071A (en) | Fermentation method for producing xylitol and ethanol by using non-detoxified cellulose raw material | |
| CN101165166B (en) | Novel use for Candida tropicalis | |
| CN105062928A (en) | Zymomonas mobilis resistant to high-concentration acetic acid and high-concentration furfural and application thereof | |
| CN102492634B (en) | High-temperature resistant yeast and application thereof | |
| CN104561132B (en) | The method that bacterium produces ethanol is mixed in the presence of a kind of inhibitor | |
| CN104109640B (en) | The raw fragrant solid state fermentation yeast of ester and application thereof are produced in one strain | |
| CN100575477C (en) | The new purposes of Issatchenkia orientalis | |
| CN104480030B (en) | One plant of high temperature resistant Spathaspora passalidarum mutant and its application in xylose fermentation ethanol | |
| CN102154128B (en) | Pichia anomala strain capable of directly preparing xylose into alcohol | |
| CN101475976A (en) | Multi-strain combined fermentation process for preparing fuel ethanol from wood fiber | |
| CN101130792A (en) | Method for producing fuel alcohol by straw enzymolysis | |
| CN105524969A (en) | A method of preparing fuel ethanol by utilizing microbes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: HENAN TIANGUAN CELLULOSIC ETHANOL CO., LTD. Free format text: FORMER OWNER: NANYANG TIANGUAN BIO FERMENTATION CO., LTD. Effective date: 20150710 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20150710 Address after: 473000 No. 1 crown Road, eco industrial park, Henan, Nanyang Patentee after: Henan Tianguan Cellulosic Ethanol Co., Ltd. Address before: Nanyang City, Henan province 474250 Zhenping county industrial park Patentee before: Nanyang Tianguan Bio Fermentation Co., Ltd. |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120801 Termination date: 20170111 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |