CN102154375B - Method for transforming straws into methane by adopting microbe method - Google Patents
Method for transforming straws into methane by adopting microbe method Download PDFInfo
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- CN102154375B CN102154375B CN2011100282150A CN201110028215A CN102154375B CN 102154375 B CN102154375 B CN 102154375B CN 2011100282150 A CN2011100282150 A CN 2011100282150A CN 201110028215 A CN201110028215 A CN 201110028215A CN 102154375 B CN102154375 B CN 102154375B
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- China
- Prior art keywords
- methane
- transforming
- straws
- glycerol
- adopting
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Links
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 title claims abstract description 32
- 239000010902 straw Substances 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 16
- 230000001131 transforming effect Effects 0.000 title abstract 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 39
- 238000000855 fermentation Methods 0.000 claims abstract description 12
- 230000004151 fermentation Effects 0.000 claims abstract description 12
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 9
- 235000009566 rice Nutrition 0.000 claims abstract description 9
- 239000010802 sludge Substances 0.000 claims abstract description 7
- 240000007594 Oryza sativa Species 0.000 claims abstract 2
- 239000007788 liquid Substances 0.000 claims description 14
- 235000015097 nutrients Nutrition 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 5
- 230000005070 ripening Effects 0.000 claims description 5
- 210000002268 wool Anatomy 0.000 claims description 5
- 229910021591 Copper(I) chloride Inorganic materials 0.000 claims description 3
- NGPGDYLVALNKEG-UHFFFAOYSA-N azanium;azane;2,3,4-trihydroxy-4-oxobutanoate Chemical compound [NH4+].[NH4+].[O-]C(=O)C(O)C(O)C([O-])=O NGPGDYLVALNKEG-UHFFFAOYSA-N 0.000 claims description 3
- OXBLHERUFWYNTN-UHFFFAOYSA-M copper(I) chloride Chemical compound [Cu]Cl OXBLHERUFWYNTN-UHFFFAOYSA-M 0.000 claims description 3
- 239000003292 glue Substances 0.000 claims description 3
- 230000000813 microbial effect Effects 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 2
- 230000002538 fungal effect Effects 0.000 claims description 2
- 238000012856 packing Methods 0.000 claims description 2
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 238000007789 sealing Methods 0.000 claims description 2
- 239000011780 sodium chloride Substances 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 230000007613 environmental effect Effects 0.000 abstract description 4
- 150000001720 carbohydrates Chemical class 0.000 abstract description 3
- 238000011161 development Methods 0.000 abstract description 3
- 230000007269 microbial metabolism Effects 0.000 abstract description 3
- 235000014633 carbohydrates Nutrition 0.000 abstract 1
- 230000000696 methanogenic effect Effects 0.000 abstract 1
- 238000013048 microbiological method Methods 0.000 abstract 1
- 241000209094 Oryza Species 0.000 description 7
- 235000013619 trace mineral Nutrition 0.000 description 4
- 239000011573 trace mineral Substances 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000013459 approach Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000036983 biotransformation Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- -1 feed Substances 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012092 media component Substances 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000000967 suction filtration Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention discloses a method for transforming straws into methane by adopting a microbiological method, belonging to the technical field of environmental biology. The method comprises the following steps of: transforming rice straws into a fermentation product mainly containing glycerol by using a fragile hair mold strain; and transforming the glycerol into methane by using methanogenic active sludge. By adopting the method, carbohydrates in straws are transformed into methane through microbial metabolism, so that reasonable, effective, environmentally-friendly and multi-way utilization of the straw resource is realized, and the sustainable development of the agriculture and the ecological environment is facilitated. The method has low equipment requirement and industrial application prospect, and is easy to operate.
Description
Technical field
The invention belongs to the Environmental Biotechnology field, be specifically related to a kind of method of utilizing microorganism stalk to be converted into methane.
Background technology
China's biomass resource is very abundant, can produce more than 600,000,000 ton of various agricultural crop straw every year, and potentiality to be exploited is huge.The utilization of China's stalk resource at present and processing mainly comprise stalk as paper industry raw material, feed, fuel, direct returning to farmland etc., but utilization ratio only accounts for 50% of total amount, having half stalk to be burned and throw aside huddles, the calorific value that produces of annual crop straw burning 500,000,000 tons of standard coals that are equivalent to burn wherein, not only waste stalk resource, also environment has been caused serious harm.To stalk resource carry out rationally effectively, environmental friendliness, multipath utilization, be directly connected to the Sustainable development of China's agricultural and ecotope.By microbial metabolism the carbohydrate in the stalk being converted into materials such as alcohols, inflammable gas is the popular approach that carries out straw utilization in recent years both at home and abroad.Yet the path for transformation of report generally all needs to carry out physics or Chemical Pretreatment at present, the secondary pollution that needs consumes energy and produce chemical reagent.
The bio-transformation approach that the present invention relates to does not need stalk is carried out the pre-treatment of physics or chemistry, adopts the method for pure biology to transform, energy-conserving and environment-protective more.
Summary of the invention
The object of the present invention is to provide a kind of method of utilizing microorganism stalk to be converted into methane.
It is the method for methane that a kind of microbial method transforms stalk, it is characterized in that, carries out according to following steps:
(1) choose ripe before, the rice straw after ripening stage and the results, be ground into the straw powder;
(2) in nutrient solution, add the straw powder, mixing, regulating the pH value is 5.0, and sterilization inserts easy break wool fungal hyphae, puts into shaking table and cultivates, and shaking speed is 120rpm, and temperature is 28 ℃, cultivates 2-3 days, obtains containing the fermented liquid of glycerol;
(3) fermented liquid that will contain glycerol is received in the substratum that contains active sludge, the vial of packing into, cultivate at shaking table, the other end of vial connects history formula fermentation tube with transfusion device, all mouths of pipe are all with glue sealing, shaking speed 140rpm, 35 ℃ of temperature, note observing level change after cultivating 2h, calculate the volume of the methane of generation by liquid level variation in the observation calculating history formula fermentation tube.
The component of described nutrient solution is KH
2PO
43g/L, (NH
4)
2SO
40.1%, MgSO
43.1g/L, ammonium tartrate 5g/L, NaCl 1g/L, FeSO
40.1g/L, CoCl
20.1g/L, ZnSO
40.1g/L, CuCl
20.1g/L, AlK (SO4)
210mg/L, H
3BO
410mg/L, Na
2MO
410mg/L.
The described nutrient media components that contains active sludge is fermented substrate, trace element solution, methanogenesis activity mud.
The component of described trace element solution is CO (NH
2)
20.44g/L, KH
2PO
40.2g/L, NaCl4mg/L, Na
2MO
44mg/L, FeCl
21.1mg/L, MnSO
41.2mg/L, MgSO
40.4g/L, CaCl
24mg/L.
Beneficial effect of the present invention: the present invention is converted into methane by microbial metabolism with the carbohydrate in the stalk, realized to stalk resource carry out rationally effectively, environmental friendliness, multipath utilization, be conducive to the Sustainable development of agricultural and ecotope.Method of the present invention is low for equipment requirements, and is simple to operate, has the industrial applications prospect.
Embodiment
The present invention will be further described with specific embodiment below:
The mould liquid state fermentation to rice straw of embodiment 1 easy break wool
Choose ripe before, the rice straw after ripening stage and the results, be ground into the straw powder; The preparation nutrient solution, component is KH
2PO
43g/L, (NH
4)
2SO
40.1%, MgSO
43.1g/L, ammonium tartrate 5g/L, NaCl1g/L, FeSO
40.1g/L, CoCl
20.1g/L, ZnSO
40.1g/L, CuCl
20.1g/L, AlK (SO
4)
210mg/L, H
3BO
410mg/L, Na
2MO
410mg/L; Add straw powder 5g/L in the nutrient solution, regulate pH 5.0, the 20min that sterilizes under 121 ℃, 0.1MPa makes liquid substratum.
Easy break wool mould silk is seeded on the PDA substratum, is under 28 ℃ in temperature, cultivates 2 days.Then, beating from colony edge with diameter 5mm punch tool and to get well-grown mycelia piece, receive in the triangular flask that the liquid substratum of 100mL is housed, is that 28 ℃, rotating speed are that the shaking table of 120rpm was cultivated 2-3 days in temperature, suction filtration is removed mycelia and stalk, and gained liquid is the fermented liquid that contains glycerol.
This bacterial strain is under above-mentioned culture condition, in 72h, before the fermenting-ripening and the glycerol output of ripening stage rice straw apparently higher than the rice straw in other vegetative period, the glycerol output of rice straw is 1205ppm/g (stalk) before ripe, and the glycerol output of ripening stage rice straw is 1119ppm/g (stalk).
Embodiment 2 active sludge are to the fermentation of glycerol
The preparation trace element solution, component is CO (NH
2)
20.44g/L, KH
2PO
40.2g/L, NaCl4mg/L, Na
2MO
44mg/L, FeCl
21.1mg/L, MnSO
41.2mg/L, MgSO
40.4g/L, CaCl
24mg/L.
Fermented liquid, 50mL fermented substrate, 1mL trace element solution, the 30mL methanogenesis activity mud that 19mL is contained glycerol is put into the vial of 150mL, mixing, cultivate at shaking table, the other end of vial connects history formula fermentation tube with transfusion device, the saturated NaCl solution of the NaOH of 2M is equipped with at the spherical position of fermentation tube, all mouths of pipe all seal with glue, shaking speed 140rpm, 35 ℃ of temperature, note observing level change after cultivating 2h, calculate the volume of the methane of generation by liquid level variation in the observation calculating fermentation tube.Calculate and produce methane efficient (U), (methane efficient is produced in the K-accumulation to U=KT0/XVrT1, and unit is mLCH
4/ h; T0-mark attitude absolute temperature, 273K; The X-sludge concentration, unit is Gvss/L; The volume of Vr-reaction flask, unit are L; Room temperature absolute temperature during the T1-test, unit is K).Experimental result shows the 0.5g stalk fermented liquid that contains glycerol that mould fermentation produces through easy break wool, and the efficient that produces methane through the methanogenesis activity sludge fermentation is 0.038mL/h (g mud).
Claims (1)
1. a microbial method conversion stalk is the method for methane, it is characterized in that, carries out according to following steps:
(1) choose ripe before, the rice straw after ripening stage and the results, be ground into the straw powder;
(2) be KH to component
2PO
43g/L, (NH
4)
2SO
40.1%, MgSO
43.1g/L, ammonium tartrate 5g/L, NaCl 1g/L, FeSO
40.1g/L, CoCl
20.1g/L, ZnSO
40.1g/L, CuCl
20.1 g/L, AlK (SO
4)
210mg/L, H
3BO
410mg/L, Na
2MO
4Add the straw powder in the nutrient solution of 10mg/L, mixing, regulating the pH value is 5.0, and sterilization inserts easy break wool fungal hyphae, puts into shaking table and cultivates, and shaking speed is 120 rpm, and temperature is 28 ℃, cultivates 2-3 days, obtains containing the fermented liquid of glycerol;
(3) fermented liquid that will contain glycerol is received in the substratum that contains active sludge, the vial of packing into, cultivate at shaking table, the other end of vial connects history formula fermentation tube with transfusion device, all mouths of pipe are all with glue sealing, shaking speed 140 rpm, 35 ℃ of temperature, note observing level change after cultivating 2h, calculate the volume of the methane of generation by liquid level variation in the observation calculating history formula fermentation tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100282150A CN102154375B (en) | 2011-01-26 | 2011-01-26 | Method for transforming straws into methane by adopting microbe method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100282150A CN102154375B (en) | 2011-01-26 | 2011-01-26 | Method for transforming straws into methane by adopting microbe method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102154375A CN102154375A (en) | 2011-08-17 |
| CN102154375B true CN102154375B (en) | 2013-07-03 |
Family
ID=44436047
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011100282150A Expired - Fee Related CN102154375B (en) | 2011-01-26 | 2011-01-26 | Method for transforming straws into methane by adopting microbe method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102154375B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111909967A (en) * | 2019-05-07 | 2020-11-10 | 湖南省土壤肥料研究所 | Method for increasing content and volume of methane gas |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1381548A (en) * | 2001-04-23 | 2002-11-27 | 张汝昆 | Process for generating combustible gas with plant straw or stalk |
| CN101691586A (en) * | 2009-09-11 | 2010-04-07 | 南京工业大学 | Method for efficiently and quickly producing biogas by anaerobic fermentation of plant straws |
| CN101768620A (en) * | 2010-02-05 | 2010-07-07 | 安徽安生生物化工科技有限责任公司 | Method for producing biogas by micro-reaction thermal-explosion straw fermentation |
| CN101818173A (en) * | 2010-03-23 | 2010-09-01 | 河南农业大学 | Process for producing biogas from crop straw by dry fermentation |
-
2011
- 2011-01-26 CN CN2011100282150A patent/CN102154375B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1381548A (en) * | 2001-04-23 | 2002-11-27 | 张汝昆 | Process for generating combustible gas with plant straw or stalk |
| CN101691586A (en) * | 2009-09-11 | 2010-04-07 | 南京工业大学 | Method for efficiently and quickly producing biogas by anaerobic fermentation of plant straws |
| CN101768620A (en) * | 2010-02-05 | 2010-07-07 | 安徽安生生物化工科技有限责任公司 | Method for producing biogas by micro-reaction thermal-explosion straw fermentation |
| CN101818173A (en) * | 2010-03-23 | 2010-09-01 | 河南农业大学 | Process for producing biogas from crop straw by dry fermentation |
Non-Patent Citations (1)
| Title |
|---|
| 吕育财等.纤维素分解菌复合系WDC2分解小麦秸秆的特性及菌群多样性.《中国农业大学学报》.2009,第14卷(第05期),40-46. * |
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| Publication number | Publication date |
|---|---|
| CN102154375A (en) | 2011-08-17 |
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Granted publication date: 20130703 Termination date: 20220126 |
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