CN102154158A - Pseudoalteromonas sp., dextran enzyme-producing method using same and dextran enzyme product - Google Patents
Pseudoalteromonas sp., dextran enzyme-producing method using same and dextran enzyme product Download PDFInfo
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Abstract
The invention relates to a Pseudoalteromonas sp. LP602 CGMCC NO.4319. The strain is characterized in that the strain is a Gram-negative rod-shaped bacterium, and has capsules but no spores; and the growth temperature range of the strain is 4-37 DEG C, the growth pH range is 6.0-11.0, and the grown NaCl concentration range is 0.5-10%. The invention also discloses a method for producing a dextran enzyme by using the Pseudoalteromonas sp. and a dextran enzyme product prepared by the method. The dextran enzyme plays an important role in the aspects of saprodontia prevention and treatment, medical dextran production and the like.
Description
Technical field
The present invention relates to a kind of microorganism, particularly a kind of pseudoalteromonas LP602(that separates from marine site, Lianyun Harbour, Jiangsu Province, China
Pseudoalteromonassp. LP602) CGMCC N0.4319(is deposited in the common micro-organisms center C GMCC of China Committee for Culture Collection of Microorganisms on November 10th, 2010, and deposit number is CGMCC N0.4319); The invention still further relates to this bacterial strain and produce method of dextran enzyme and products thereof.
Background technology
Dextran (Dextean) mainly is α-1, the glucose that the 6-glycosidic link connects.(6-Glucan-6-D-Glucanohydrolase EC3.2.1.11), is alpha-glucanase again to the dextran enzyme for Dextranase, α-D-1, is the lytic enzyme of α-1,6 glycosidic link in the single-minded cutting dextran.
At present both at home and abroad the generation dextran enzyme microorganism of report mainly be mould (
Penicilliumsp.), this reach saccharomyces oleaginosus (
Lipomyces starkeyi), the diagonal angle chaetomium (
Chaetomium gracile), aspergillus (
Aspergillus ustus), genus bacillus (
BacillusSp.), suis (
StreptococcusSp.) all can produce the dextran enzyme.
The dextran enzyme has very important use value, and the dextran enzyme is the degradation of polysaccharide polymkeric substance in sugar industry, reduces the relative molecular mass of polysaccharide, thereby reduces the stickiness of sugar.The dextran enzyme can also be used for the Production by Enzymes of oral medical goods and plasma substitute dextran.
The application of dextran enzyme in the oral medical goods.Sucrose in the fermentation using bacteria food in the oral cavity, and the generation dextran (α-D-glucan).Dextran forms plaque at the tooth face, and it is to cause the determinative of bacterium in the dental surface accumulation.Then breeding in a large number under reticulated structure protection of microorganism in the bacterial plaque, its acidic metabolite destroys enamel, thereby causes carious tooth, and causing can't the regenerated injury of teeth, also brings huge misery to the patient simultaneously.Plaque is one of important paathogenic factor of carious tooth and periodontopathy.Therefore in order to prevent and control carious tooth and periodontopathy, the generation and the development that suppress plaque are vital.
Dextran is made of α-1,4, α-1,3 and α-1,6 glycosidic link, and wherein the side chain of α-1,6 glycosidic link formation determines its adhesivity, and this adhesivity is the important materialization factor that forms bacterial plaque and carious tooth.Dextran is cross-linked with each other by its adhesivity, forms the protectiveness reticulated structure, feasible wherein microorganism, and the suis that particularly has carious tooth formation ability is very easy to grow.
Because dextran forms firm in dental surface or slit and has certain flexible bacterial plaque structure; this protective structures makes to have very difficult elimination of microorganism that carious tooth forms ability, and Physiotherapy methods such as therefore traditional toothbrush, dental floss are difficult to stop carious lesions.Usually contain compounds such as boric acid, metronidazole, cetylpyridinium chloride, chlorhexidine, the tincture of iodine, ethanol in the common chemical oral cavity nursing agent, generally and under the situation of oral mucosa breakage, these materials may cause side reaction, increase patient's misery.
The dextran enzyme dextran in the oral cavity of can degrading.The dextran enzyme is a kind of biotechnological formulation, uses and preserves simple and easy to do.Nontoxicity has no drug resistance, and does not influence intraoral biological and ecological balance, under the infant that can not brush teeth and the situation that do not possess the condition of washing one's face and rinsing one's mouth, is a kind of preparation of good preventing tooth carious tooth spot.And everyone after meal, especially uses the mouth wash shua that is added with this enzyme after the sugar, is that a good restraining bacterial plaque forms the effective measure of preventing dental caries and periodontopathy.Various countries had done further investigation in this field in recent years, and the dextran enzyme with degradation bacteria dental plaque ability is added in toothpaste, collutory and the chewing gum, had obtained the good effect of preventing and treating carious tooth.
The application of dextran enzyme in the Production by Enzymes of plasma substitute dextran.Dextran is mainly as blood plasma extender, i.e. plasma substitute.At present pharmaceutically the dextran of usefulness has two kinds of middle molecular weight and lower molecular weights, and middle molecular weight is limited to about 70000, and low-molecular-weight is 40000.At present, the production of medicinal dextran mainly is to adopt acid-hydrolysis method, the condition fierceness, and yield is low, requires production unit condition harshness, if can adopt Production by Enzymes, can improve productive rate, reduces cost.
The research to these two fields abroad all has report with application, but domestic present correlative study is less, does not have the relevant patent that marine source dextran enzyme is applied to medical articles through Cha Xinshang.And the production bacterial strain of external dextran enzyme preparation mostly is the fungi of Lu Yuan, for example
Lipomyces starkeyiDeng, its enzymic activity parameter is not satisfactory.This is because the dextran enzyme optimum temperuture of Lu Yuan is higher, and plays a role under comparatively gentle acid-base condition, not organic solvent-resistant.And the dextran enzyme of marine source can under extreme conditions play a role, for example low temperature, extreme pH, high salt, organic solvent, and this provides optimal conditions for developing more novel sea bio-medical materials.Current marine microorganism produced the development research that the dextran enzyme is used for biomedical articles both at home and abroad and do not appear in the newspapers as yet.
Though China zymin market growth is very fast, the research of marine source zymin is not enough.China's zymin research focuses mostly in food, industry, agricultural, but not enough for pharmaceutical enzyme research.China starts late for zymologic property research and industrial applications research, the especially applied research on biomedicine of the dextran enzyme of marine source.
Summary of the invention
Technical problem to be solved by this invention is at the deficiencies in the prior art, provides a kind of new marine bacteria that can produce the dextran enzyme for pseudomonas LP602.
Another technical problem to be solved by this invention is the growth characteristics of research pseudoalteromonas, and produces the method and the enzyme product of dextran enzyme.
Technical problem to be solved by this invention is to realize by following technical scheme.
Feature of the present invention comprises pseudoalteromonas LP602(
Pseudoalteromonassp.LP602) bacterial strain itself, and the method for utilizing this bacterial strain to produce the dextran enzyme, and the dextran enzyme product that utilizes this bacterial strain to produce.
Bacterial strain involved in the present invention is the pseudoalteromonas LP602(that is separated in the seawater in marine site, Lianyun Harbour, Jiangsu Province, China
Pseudoalteromonas sp.LP602), this pseudoalteromonas LP602 is deposited in the common micro-organisms center C GMCC of China Committee for Culture Collection of Microorganisms on November 10th, 2010, deposit number is CGMCC N0.4319, and the preservation address is: No. 3 Institute of Microorganism, Academia Sinica in Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.
One, the screening method of bacterial strain of the present invention.
1.1 the substratum that relates among the present invention:
2216E substratum: peptone 0.5%, yeast powder 0.1%, agar 2%, Chen Haishui preparation, pH8.
The primary dcreening operation substratum: peptone 1%, extractum carnis 0.5%, dextran 0.2%, agar 2%, the Chen Haishui preparation, pH 8.0;
Produce the enzyme substratum: yeast extract paste 0.1%, peptone 0.1%, dextran 1%, the Chen Haishui preparation, pH 8.0.
Trace quantity mineral salts solution (every liter): CuSO
45H
2O, 0.01g; ZnSO
47H
2O, 0.1g; CoCl
26H
2O, 0.005g; MnCl
24H
2O, 0.2g; Na
2MoO
42H
2O, 0.1g; KBr, 0.05g; KI, 0.05g; H
3B0
3,0.1g; NaF, 0.05g; LiCl, 0.05g; Al
2(SO4)
3,0.05g; NiCl
26H
2O, 0.01g; VoSO
42H
2O, 0.005g; H
2WO
42H
2O, 0.002g; Na
2SeO
4,0.005g; SrCl6H
2O, 0.005g; BaCl
2, 0.005g.
1.2 the screening method of bacterial strain:
Ooze is directly got 1g and is put into 50ml 2216E substratum, and 20 ℃, 180r/min are cultivated 2-5d.Choose the diluent coating primary dcreening operation substratum of suitable nutrient solution, cultivate 3-4d for 20 ℃, bacterium colony grows the back and adds 95% ethanol, and-20 ℃ of freezing 3 ~ 4h observe periphery of bacterial colonies and transparent circle whether occurs.Picking has the single bacterium colony bacterial strain of transparent circle to insert to produce the enzyme substratum, and 20 ℃, 180r/min are cultivated 2d, and the centrifugal 15min of 10000r/min gets supernatant liquor mensuration enzyme activity size.Select the big and higher LP602 bacterial strain of enzyme activity of transparent circle.
Two, the morphological specificity of bacterial strain of the present invention and physiological and biochemical property.
1.1 morphological specificity:
Bacterial strain is the Gram-negative rod-shaped bacterium, has pod membrane, no gemma, size to be about 1.7-2 μ m * 0.8-1.2 μ m, referring to Fig. 1.At the colony characteristics that contains on the dextran solid medium: colony diameter 4 mm-7 mm, circle, oyster white, moistening, the edge is smooth, center protrusion, bacterium colony grows the back and adds 95% ethanol ,-20 ℃ of freezing 3 ~ 4h, transparent circle appears in periphery of bacterial colonies, referring to Fig. 2.
1.2 physiological and biochemical property:
The physiological and biochemical property of pseudoalteromonas LP602 and with other pseudoalteromonas relatively see Fig. 3,4.Bacterial strain LP602 oxydase is positive, energy glucose fermentation, methyl red, and V-P tests negative, and non-sodium chloride can not be grown, and the energy liquefy gelatin can not produce indoles; Glycerine, L-Ala, L-glutamic acid, pyruvic acid can be utilized, and lactose, semi-lactosi, fructose, cellobiose, maltose can not be utilized.Pass through Bergey ' s Manual of Systematic Bacteriology (second edition) and analyze relatively, this bacterial strain of preliminary judgement LP602 be pseudoalteromonas belong to (
Pseudoalteromonassp.).
1.3 the molecular biology identification of bacterial strain LP602:
Extract the DNA template with the Takara test kit, and in-40 ℃ of preservations.
Forward primer P1:5 '-GAGAGTTTGATCCTGGCT-3 ', reverse primer P2:5 '-CGGCTACCTTGTTACGAC-3 '.Reaction system 25 μ l, Taq enzyme 2U, reaction conditions are 95 ℃ of pre-sex change 5min, 94 ℃ of sex change 30s, and 53 ℃ of annealing 45s, 72 ℃ are extended 70s, 35 circulations, 72 ℃ are extended 6min.The purifying of PCR product, clone, order-checking are finished by Shanghai Sangon Biological Engineering Technology And Service Co., Ltd.The 16S rDNA of amplification bacterial strain LP602, its length is 1500bp.Submit the 16S rRNA gene order of bacterial strain LP602 to the GenBank database, by 16S rDNA sequence homology relatively, can tentatively confirm this bacterial strain be pseudoalteromonas belong to (
PseudoalteromonasSp.).Bacterial strain 16S rDNA that sibship is nearer uses MEGA software and carries out multiple comparisons, with in adjacent method (Neibor-joining method) build systematic evolution tree, show bacterial strain LP602 and pseudoalteromonas genus (NR028992 from evolutionary tree
Pseudoalteromonas mariniglutinosa) be positioned at the cluster group, sibship is nearest, referring to Fig. 5.
Three,The growth characteristics of bacterial strain LP602 of the present invention
Bacterial strain LP602 provided by the invention has carried out careful research to its growth characteristics, understands the growing state of this bacterium under the different condition.
3.1 the preparation of seed liquor: the pseudoalteromonas LP602 that will be deposited in the 2216E substratum is inoculated in the 2216E substratum, rotating speed 180r/min, and liquid amount 20% is cultivated 12h.
3.2 the influence that temperature is grown to bacterial strain LP602:
With seed liquor with 2% inoculum size in the 2216E substratum, rotating speed 180r/min, liquid amount 20%, under differing temps, cultivate respectively, measure cell concn at set intervals, be chosen in and measure the OD value under the 600nm wavelength, this bacterial strain can be 4 ℃ of growths down, its growth temperature range is 4～37 ℃, and optimum growth temperature is 25 ℃, sees Fig. 6.
3.3pH influence to bacterial strain LP602 growth:
Adding final concentration in the 2216E substratum is the phosphoric acid of 10mM: acetic acid: boric acid (1:1:1) is respectively between 4.0～11.0 the pH of substratum as damping fluid, cultivates 24 hours at 25 ℃, measure cell concn, growth pH scope is 6～11, and the suitableeest growth pH is 9.0, sees Fig. 7.
3.4 the influence that NaCl grows to bacterial strain LP602:
Prepare seed liquor according to 3.1 methods, (using Chen Haishui instead the trace quantity mineral salts solution replaces at the 2216E substratum, every liter of substratum adds 10ml) the middle NaCl of adding, making it is 0% to 12% NaCl, cultivated 24 hours at 25 ℃, measure cell concn, the NaCl concentration of growth is 0.5%～10%, the NaCl concentration of suitable growth is 7%, sees Fig. 8.
Four, bacterial strain produces the method for dextran enzyme.
Seed liquor is inoculated in 2% inoculum size produces the enzyme substratum, 180r/min cultivates 24h, the centrifugal 10min of 10000r/min for 25 ℃, get supernatant liquor, add 65% ammonium sulfate, leave standstill 3h after, the centrifugal 20min of 12000r/min, use an amount of 50mM, pH is 7.0 Tris-HCL damping fluid dissolution precipitation, and 4 ℃ of dialysis 24h obtain the dextran enzyme through methods such as DEAE-sepharose Flat Flow and Sephacryl S-200 more then.
Five, the character of dextran enzyme.
5.1 the enzyme operative temperature is to the influence of enzymic activity:
The dextran enzyme placed under the differing temps react with substrate, measure enzyme activity, the results are shown in Figure 9, the optimum temperature of enzyme is 20 ℃, and 5 ℃ still have 45% enzyme activity, and higher catalysis activity is arranged when 5 ℃～40 ℃ temperature ranges.
5.2 the thermostability of enzyme:
Get an amount of enzyme liquid and be incubated 2.5h down in differing temps (30 ℃, 40 ℃, 50 ℃ and 80 ℃) respectively, take out one group of sample every 0.5 h, place rapidly in 4 ℃ of refrigerators, treat to be unified under the standard conditions after insulation finishes and measure remnant enzyme activity, enzyme work with the enzyme liquid that is untreated is made as 100%, the results are shown in Figure 10, the better heat stability of the dextran enzyme of generation, enzyme is lived and still can be kept more than 74% behind insulation 2.5 h under 50 ℃, and enzyme is alive behind insulation 2.5 h under 80 ℃ still can keep 50%.
5.3 the action pH of enzyme is to the influence of enzymic activity:
With enzyme liquid with in 1% the dextran solution of different pH, under 20 ℃, carry out enzyme activity determination, the damping fluid of different pH values is: 50mM sodium citrate buffer solution (pH3.0 to 6.0); 50mM sodium phosphate buffer (pH 6.0 to 8.0); 50mM Tris-hydrochloride buffer (pH 8.0 to 9.0); 50mM glycine-sodium hydrate buffer solution (pH9.0 to 10.0).The results are shown in Figure 11, the activity of this enzyme of pH8.0 is the highest.
5.4 the pH stability of enzyme:
50 ul enzyme liquid are mixed with the damping fluid of the different pH of 150 ul, damping fluid be Bloomsbury smooth-Robinson, Robert (Britton-Robinson) buffered soln, pH is respectively 4.0,5.0,6.0,7.0,8.0,9.0,10.0, insulation 1h take out to measure remnant enzyme activity in 20 ℃ of water-baths, and the enzyme work of the enzyme liquid that is untreated is made as 100%.The results are shown in Figure 12, the result shows that the dextran enzyme is stable in pH7.0～9.0 scopes behind 1h under 20 ° of C insulations, and residual enzyme activity remains on more than 55%, at pH4.0 and pH10.0 8.28% and 21.6% residual enzyme activity is arranged respectively.
5.5 dextran enzyme activity determination
1. dextran enzyme activity determination method: get 1% dextran solution, 100 μ L and add 100 μ L enzyme liquid, blank enzyme liquid with 100 μ L deactivations is for it, 20 ℃ of reaction 15min, put into the ice bath termination reaction immediately, add 200 μ L DNS and boil 5min for 100 ℃, termination reaction and colour developing add 3mL deionized water vibration mixing, get 200 μ L and carry out light absorption value mensuration on 96 hole enzyme plates under 520nm.
2. enzyme activity unit definition: per minute hydrolysis dextran produces the required enzyme amount of 1 μ g maltose under the above-mentioned reaction conditions, is defined as an enzyme activity unit (U).
Description of drawings
Fig. 1 is bacterial strain LP602 form dyeing characteristic (10 * 100) figure.
Fig. 2 is that bacterial strain LP602 is at the transparent loop graph that contains on the flat board of dextran.
Fig. 3 is the physiological and biochemical property chart of bacterial strain LP602.
Fig. 4 is the comparison diagram of bacterial strain LP602 physicochemical characteristics and other pseudoalteromonas.
Fig. 5 is the 16S rRNA evolutionary tree analysis chart of bacterial strain LP602.
Fig. 6 is the influence figure of temperature to bacterial strain LP602 growth.Among the figure: 15 ℃ (◆), 20 ℃ (), 25 ℃ (▲), 30 ℃ (■).
Fig. 7 is the influence figure of pH to bacterial strain LP602 growth.
Fig. 8 is the influence figure of NaCl concentration to strain growth.
Fig. 9 is the influence figure of temperature to enzymic activity.
Figure 10 is the influence figure of temperature to enzyme heat stability.Among the figure: 30 ℃ (◇), 40 ℃ (*), 50(◆), 80 ℃ (■).
Figure 11 is the influence figure of pH to enzyme activity.
Figure 12 is the influence figure of pH to enzyme stability.
Bacterial strain pseudoalteromonas LP602(involved in the present invention
Pseudoalteromonassp.LP602) be deposited in the common micro-organisms center C GMCC of China Committee for Culture Collection of Microorganisms on November 10th, 2010, deposit number is: CGMCC N0.4319; The preservation address is: No. 3 Institute of Microorganism, Academia Sinica in Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.
Embodiment
Following with reference to accompanying drawing, further describe concrete technical scheme of the present utility model, so that those skilled in the art understands the present invention further, and do not constitute restriction to its right.
Embodiment 1.A kind of pseudoalteromonas LP602(
Pseudoalteromonassp.LP602) CGMCC N0.4319.This bacterial strain has following feature: bacterial strain is the Gram-negative rod-shaped bacterium, has pod membrane, no gemma, size to be about 1.7-2 μ m * 0.8-1.2 μ m; At the colony characteristics that contains on the dextran solid medium: colony diameter 4 mm-7 mm, circle, oyster white, moistening, the edge is smooth, center protrusion, bacterium colony grows the back and adds 95% ethanol ,-20 ℃ of freezing 3 ~ 4h, transparent circle appears in periphery of bacterial colonies; Oxydase is positive, energy glucose fermentation, methyl red, and V-P tests negative, and non-sodium chloride can not be grown, and the energy liquefy gelatin can not produce indoles; Glycerine, L-Ala, L-glutamic acid, pyruvic acid can be utilized, and lactose, semi-lactosi, fructose, cellobiose, maltose can not be utilized.The growth characteristics of pseudoalteromonas LP602 are: this strain growth temperature range is 4～37 ℃, growth pH scope is 6.0～11.0, and the NaCl concentration range of growth is 0.5%～10%, and optimum growth temperature is 25 ℃, the suitableeest growth pH is 9.0, and the NaCl concentration of suitable growth is 7%.
Embodiment 2.A kind of embodiment 1 described pseudoalteromonas LP602 (
Pseudoalteromonassp.LP602) CGMCC N0.4319 produces the method for dextran enzyme, and its step is as follows: with pseudoalteromonas LP602 inoculation in the 2216E substratum, rotating speed 180r/min, liquid amount 20% is cultivated 12h, seed liquor; Seed liquor is inoculated in fermention medium with 2% inoculum size, and 180r/min cultivates 24h for 25 ℃, and the centrifugal 5min of 10000r/min gets supernatant liquor and is dextran enzyme crude enzyme liquid.
Embodiment 3.A kind of embodiment 2 described methods are produced the dextran enzyme, this dextran enzyme has following feature: the character of described dextran enzyme is: the suitable operative temperature of dextran enzyme is 20 ℃, 5 ℃ still have 45% enzyme activity, higher catalysis activity is arranged when 5 ℃～40 ℃ temperature ranges, the better heat stability of the dextran enzyme that produces, enzyme is lived and still can be kept more than 74% behind insulation 2.5 h under 50 ℃, and enzyme is alive behind insulation 2.5 h under 80 ℃ still can keep 50%.The dextran enzyme is stable in pH7.0～9.0 scopes.
Claims (6)
1. pseudoalteromonas LP602(
Pseudoalteromonassp.LP602) CGMCC N0.4319.
2. according to the described pseudoalteromonas LP602 of claim 1 CGMCC N0.4319, it is characterized in that this bacterial strain has following feature: bacterial strain is the Gram-negative rod-shaped bacterium, pod membrane, no gemma, size are arranged is 1.7-2 μ m * 0.8-1.2 μ m; At the colony characteristics that contains on the dextran solid medium be: colony diameter 4 mm-7 mm, circle, oyster white, moistening, the edge is smooth, center protrusion, bacterium colony grows the back and adds 95% ethanol ,-20 ℃ of freezing 3 ~ 4h, transparent circle appears in periphery of bacterial colonies; Oxydase is positive, energy glucose fermentation, methyl red, and V-P tests negative, and non-sodium chloride can not be grown, and the energy liquefy gelatin can not produce indoles; Glycerine, L-Ala, L-glutamic acid and pyruvic acid can be utilized, and lactose, semi-lactosi, fructose, cellobiose and maltose can not be utilized.
According to the described pseudoalteromonas LP602 of claim 1 (
Pseudoalteromonassp.LP602) CGMCC N0.4319 is characterized in that, its growth characteristics are: this strain growth temperature range is 4～37 ℃, and growth pH scope is 6.0～11.0, and the NaCl concentration range of growth is 0.5%～10%.
According to the described pseudoalteromonas LP602 of claim 3 (
Pseudoalteromonassp.LP602) CGMCC N0.4319 is characterized in that: this bacterial strain optimum growth temperature is 25 ℃, and the suitableeest growth pH is 9.0, and the NaCl concentration of suitable growth is 7%.
One kind as among the claim 1-4 any one described as described in pseudoalteromonas LP602(
Pseudoalteromonassp.LP602) CGMCC N0.4319 produces the method for dextran enzyme, it is characterized in that its step is as follows: with pseudoalteromonas LP602 inoculation in the 2216E substratum, rotating speed 180r/min, liquid amount 20% is cultivated 16h, seed liquor; Seed liquor is inoculated in 2% inoculum size produces the enzyme substratum, 180r/min cultivates 24h for 25 ℃, and the centrifugal 5min of 10000r/min gets supernatant liquor and is dextran enzyme crude enzyme liquid.
6. a method as claimed in claim 5 is produced the dextran enzyme, it is characterized in that, the physico-chemical property of described dextran enzyme is: the suitable operative temperature of dextran enzyme is 20 ℃, 5 ℃ still have 45% enzyme activity, catalysis activity is arranged when 5 ℃ ~ 40 ℃ temperature ranges, the Heat stability is good of the dextran enzyme that produces, enzyme is lived and still can be kept more than 74% behind insulation 2.5 h under 50 ℃, and enzyme is alive behind insulation 2.5 h under 80 ℃ still can keep 50%; This enzyme is stable in the scope of pH 7.0～9.0.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103194414A (en) * | 2013-04-22 | 2013-07-10 | 淮海工学院 | Marine catenovulumsp. DP03 and method for producing dextran enzyme by using same |
| CN109456898A (en) * | 2018-07-09 | 2019-03-12 | 江南大学 | A kind of the fermentation preparation and its application of chaetomium globosum dextranase |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101070526A (en) * | 2007-02-12 | 2007-11-14 | 淮海工学院 | Method for producing low-temperature alkaline protease by alternative pseudomonad |
| CN101200700A (en) * | 2007-11-21 | 2008-06-18 | 淮海工学院 | Ocean low-temperature cellulase and enzyme producing method as well as producing strain pseudoalteromonas thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101070526A (en) * | 2007-02-12 | 2007-11-14 | 淮海工学院 | Method for producing low-temperature alkaline protease by alternative pseudomonad |
| CN101200700A (en) * | 2007-11-21 | 2008-06-18 | 淮海工学院 | Ocean low-temperature cellulase and enzyme producing method as well as producing strain pseudoalteromonas thereof |
Non-Patent Citations (1)
| Title |
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| 《微生物学杂志》 20101130 吕明生等 交替假单胞菌LP621菌株产右旋糖苷酶的培养条件优化 11-17 1-6 第30卷, 第6期 2 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103194414A (en) * | 2013-04-22 | 2013-07-10 | 淮海工学院 | Marine catenovulumsp. DP03 and method for producing dextran enzyme by using same |
| CN103194414B (en) * | 2013-04-22 | 2014-05-21 | 淮海工学院 | Marine catenovulumsp. DP03 and method for producing dextran enzyme by using same |
| CN109456898A (en) * | 2018-07-09 | 2019-03-12 | 江南大学 | A kind of the fermentation preparation and its application of chaetomium globosum dextranase |
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