CN108103081A - A kind of acetaldehyde dehydrogenase and its application in terms of alcohol-neutralize healthy product - Google Patents
A kind of acetaldehyde dehydrogenase and its application in terms of alcohol-neutralize healthy product Download PDFInfo
- Publication number
- CN108103081A CN108103081A CN201810148121.9A CN201810148121A CN108103081A CN 108103081 A CN108103081 A CN 108103081A CN 201810148121 A CN201810148121 A CN 201810148121A CN 108103081 A CN108103081 A CN 108103081A
- Authority
- CN
- China
- Prior art keywords
- acetaldehyde dehydrogenase
- gene
- alcohol
- acetaldehyde
- dehydrogenase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108010081577 aldehyde dehydrogenase (NAD(P)+) Proteins 0.000 title claims abstract description 32
- 241000894006 Bacteria Species 0.000 claims abstract description 16
- 238000000855 fermentation Methods 0.000 claims abstract description 16
- 230000004151 fermentation Effects 0.000 claims abstract description 16
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 11
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- 108090000623 proteins and genes Proteins 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 6
- 235000013305 food Nutrition 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 239000013604 expression vector Substances 0.000 claims description 2
- 108090000471 glyoxylate dehydrogenase (acylating) Proteins 0.000 claims description 2
- 230000036541 health Effects 0.000 claims description 2
- 239000002773 nucleotide Substances 0.000 claims description 2
- 125000003729 nucleotide group Chemical group 0.000 claims description 2
- 238000010353 genetic engineering Methods 0.000 claims 3
- 102000004190 Enzymes Human genes 0.000 abstract description 21
- 108090000790 Enzymes Proteins 0.000 abstract description 21
- 239000002609 medium Substances 0.000 abstract description 6
- 239000001110 calcium chloride Substances 0.000 abstract description 3
- 229910001628 calcium chloride Inorganic materials 0.000 abstract description 3
- 229910000366 copper(II) sulfate Inorganic materials 0.000 abstract description 3
- 239000003814 drug Substances 0.000 abstract description 3
- 239000001963 growth medium Substances 0.000 abstract description 3
- 230000002075 anti-alcohol Effects 0.000 abstract description 2
- 238000013459 approach Methods 0.000 abstract description 2
- 238000010276 construction Methods 0.000 abstract description 2
- 229940079593 drug Drugs 0.000 abstract description 2
- 230000006872 improvement Effects 0.000 abstract description 2
- 238000012827 research and development Methods 0.000 abstract description 2
- IKHGUXGNUITLKF-UHFFFAOYSA-N Acetaldehyde Chemical compound CC=O IKHGUXGNUITLKF-UHFFFAOYSA-N 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- 239000000047 product Substances 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 235000019441 ethanol Nutrition 0.000 description 5
- 239000012530 fluid Substances 0.000 description 5
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 4
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 4
- 108010009513 Mitochondrial Aldehyde Dehydrogenase Proteins 0.000 description 4
- 239000013612 plasmid Substances 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 3
- 101001045232 Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) Acetaldehyde dehydrogenase 2 Proteins 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000276408 Bacillus subtilis subsp. subtilis str. 168 Species 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 102000005369 Aldehyde Dehydrogenase Human genes 0.000 description 1
- 108020002663 Aldehyde Dehydrogenase Proteins 0.000 description 1
- 102000009645 Mitochondrial Aldehyde Dehydrogenase Human genes 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- NBXMJDVWESETMK-UHFFFAOYSA-N acetaldehyde Chemical compound CC=O.CC=O NBXMJDVWESETMK-UHFFFAOYSA-N 0.000 description 1
- IKHGUXGNUITLKF-XPULMUKRSA-N acetaldehyde Chemical compound [14CH]([14CH3])=O IKHGUXGNUITLKF-XPULMUKRSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000005554 pickling Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 230000001755 vocal effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
- C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
- C12Y102/0101—Acetaldehyde dehydrogenase (acetylating) (1.2.1.10)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A kind of application the invention discloses acetaldehyde dehydrogenase and its in terms of alcohol-neutralize healthy product, belongs to technical field of enzyme engineering.The present invention provides it is a kind of can be with the engineering bacteria and construction method of high efficient expression people's acetaldehyde dehydrogenase gene, expression of the people source acetaldehyde dehydrogenase gene in bacillus subtilis shown in SEQ ID NO.1 is realized, making fermentation, recombinant bacterium yield of enzyme reaches 24.52U/L for 24 hours.And pass through improvement fermentation medium, the yield of acetaldehyde dehydrogenase is improved, addition is made to add CaCl2Salt and CuSO4Culture medium in yield of enzyme improve to original 140~180%, provide a new approach and theoretical foundation for the research and development of antialcoholic drug.
Description
Technical field
A kind of application the present invention relates to acetaldehyde dehydrogenase and its in terms of alcohol-neutralize healthy product belongs to enzyme engineering technology neck
Domain.
Background technology
In human body alcohol dehydrogenase (Alcohol dehydrogenase) and acetaldehyde dehydrogenase (EC 1.2.1.10,
Aldehyde dehydrogenase) it is played an important role in the metabolism of ethyl alcohol.Ethyl alcohol is under the action of alcohol dehydrogenase
Acetaldehyde is oxidized to, is oxidized to acetic acid under the action of acetaldehyde dehydrogenase afterwards, when two kinds of enzymes can give full expression in human body, ethyl alcohol
It will fully be metabolized in a short time, reduce the influence of alcohol Central nervous.However, under normal circumstances, alcohol dehydrogenase can
It gives full expression to, lacks the individual relatively more of acetaldehyde dehydrogenase, acetaldehyde is caused largely to accumulate in vivo, generates drunk symptom.In people
In vivo, the acetaldehyde dehydrogenase gene for having obtained identification shares 12 kinds, and wherein acetaldehyde dehydrogenase I, acetaldehyde dehydrogenase II, acetaldehyde takes off
Hydrogen enzyme III and acetaldehyde dehydrogenase IV are four kinds of common types.Participate in oxidation of acetaldehyde acetaldehyde dehydrogenase there are mainly two types of, i.e., with
Acetaldehyde has the mitochondria acetaldehyde dehydrogenase II of most low km value and has the endochylema acetaldehyde dehydrogenase I of moderate affinity with acetaldehyde,
So acetaldehyde dehydrogenase II is the key enzyme of human body alcohol metabolism.In recent years, have the successful expression acetaldehyde in Escherichia coli to take off
The case of hydrogen enzyme, but since Escherichia coli have food-safety problem, it is not suitable for the preparation of food.As people are complete to obtaining
The demand of new, more effective medicament for sobering up and protecting liver is increasingly urgent, realizes the industrialization of II encoding gene ALDH2 of acetaldehyde dehydrogenase
Production has important application value.
The content of the invention
First purpose of the present invention is to provide a kind of gene of encoding human source acetaldehyde dehydrogenase, and nucleotide sequence is such as
Shown in SEQ ID NO.1.
Second object of the present invention is to provide the carrier or cell line for carrying the gene.
Third object of the present invention is to provide a kind of bacillus subtilis engineering bacteria, is the expression acetaldehyde dehydrogenase base
The bacillus subtilis of cause.
In one embodiment of the invention, the bacillus subtilis engineering bacteria is with bacillus subtilis
Bacillus subtilis 168 are host, and the gene of encoding glyoxylate dehydrogenase is expressed using pMA5 as expression vector.
Fourth object of the present invention is to provide a kind of production method of acetaldehyde dehydrogenase, is to be seeded to the engineering bacteria
In fermentation medium, 28~37 DEG C of 12~36h of culture.
In one embodiment of the invention, the fermentation medium is LB culture mediums.
In one embodiment of the invention, the fermentation medium also contains：CaCl20.2~0.5g/L.
In one embodiment of the invention, the fermentation medium also contains：CuSO40.2~0.5g/L.
The 5th purpose of the present invention is to provide application of the gene in the product containing acetaldehyde dehydrogenase is prepared.
In one embodiment of the invention, the product includes food, health products.
Advantageous effect：The present invention provides it is a kind of can be with the engineering bacteria and structure of high efficient expression people's acetaldehyde dehydrogenase gene
Method, the present invention realize expression of the people source acetaldehyde dehydrogenase gene in bacillus subtilis, make fermentation recombinant bacterium producing enzyme for 24 hours
Amount reaches 24.52U/L.And pass through improvement fermentation medium, the yield of acetaldehyde dehydrogenase is improved, addition is made to add CaCl2Salt and
CuSO4Culture medium in yield of enzyme improve to original 140~180%, provide a new approach for the research and development of antialcoholic drug
And theoretical foundation.
Description of the drawings
Fig. 1 is the variation diagram of recombined bacillus subtilis yield of enzyme in fermentation process.
Specific embodiment
Embodiment 1
Synthesize the ALDH2 genes shown in SEQ ID NO.1；Activation, culture bacillus subtilis 168, are inoculated in LB cultures
In base, culture 18~extract afterwards for 24 hours plasmid pMA5.By ALDH2 genes and pMA5 double digestions and connect, construction recombination plasmid
pMA5-aldh2；Recombinant plasmid pMA5-aldh2 is converted into 168 competent cell of bacillus subtilis, in LB culture mediums
Culture, is coated on LB tablets, picking positive colony, extraction plasmid and sequence verification.Correct bacterial strain is sequenced as recombinant bacterium.
Embodiment 2
Recombined bacillus subtilis prepared by embodiment 1 is inoculated in LB culture mediums, carries out activation culture in 37 DEG C, then
Two bacterium solutions are transferred simultaneously and are cultivated in fresh LB culture mediums, concrete operation step is as follows：
(1) actication of culture
The original bacteria that glycerol tube preserves is taken to be added in 30 μ L of bacterium are integrated in the test tube of 10mL culture mediums containing LB respectively, 37
DEG C, 14~20h is cultivated under the conditions of 200rpm, obtain seed liquor.
(2) strain fermentation
The seed liquor 2mL of activation is taken to be added in the LB culture mediums of 200mL respectively, is fermented under the conditions of 37 DEG C, 200rpm
For a period of time, fermentation for 24 hours, 30h, 34h and 48h when take respectively 5mL zymotic fluid be used for than measure living.
(3) processing of zymotic fluid
The zymotic fluid of 5mL centrifuges 5min under the conditions of 8000rpm, with sterile water washing thalline once, then uses liquid nitrogen frozen
Thalline is eventually adding the PEB solution of the pickling glass pearl isometric with thalline and 600 μ L, 30s is shaken on turbula shaker, it
1min is placed on ice afterwards, whole process continues 45min, and centrifugation stays supernatant to measure enzyme activity.
(4) enzyme activity and the measure of protein content
Enzyme activity defines：The enzyme amount used in ALDH2 catalysis acetaldehyde-dehydrogenase generation acetic acid is measured at 340nm.It defines per minute
It is 1 unit of activity (U) that absorbance, which increases by 0.001,
1 enzyme activity determination reaction system of table
The results are shown in Figure 1 for enzyme activity determination, and when fermenting for 24 hours, the ratio work highest of engineered strain production acetaldehyde dehydrogenase reaches
24.52U/L。
Embodiment 3
By the identical method culture bacterial strain of embodiment 2, difference lies in the metal salts of addition 0.4mmol/L into culture medium
(table 2).Fermentation measures enzyme activity afterwards for 24 hours.Using the enzyme activity fermented for 24 hours under 2 the same terms of embodiment as 100%, as a result such as 2 institute of table
Show, add Ca2+Salt and Cu2+Salt can improve the yield of the enzyme in zymotic fluid to a certain extent.Due to Ca2+Salt and Cu2+Belong to pair
The harmless or even beneficial metal ion of human body, preparing the product containing acetaldehyde dehydrogenase for field of food, there is important application to anticipate
Justice.
Enzyme activity variation in unit zymotic fluid of the table 2 containing different metal ions
Although the present invention has been described by way of example and in terms of the preferred embodiments, it is not limited to the present invention, any to be familiar with this skill
The people of art without departing from the spirit and scope of the present invention, can do various change and modification, therefore the protection model of the present invention
Enclosing be subject to what claims were defined.
SEQUENCE LISTING
<110>Zibo Vocational College
<120>A kind of acetaldehyde dehydrogenase and its application in terms of alcohol-neutralize healthy product
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1551
<212> DNA
<213>Artificial sequence
<400> 1
atgcttcgtg ctgctgctcg tttcggccct cgtcttggcc gtcgtcttct ttctgctgct 60
gctacacaag ctgttcctgc tcctaaccaa caacctgaag ttttctgcaa ccaaatcttc 120
atcaacaacg aatggcatga tgctgtttct cgtaaaacat tccctacagt taaccctttc 180
acaggcgaag ttatctgcca agttgctgaa ggcgataaag aagatgttga taaagctgtt 240
aaagctgctc gtgctgcttt ccaacttggc tctccttggc gtcgtatgga tgcttctcat 300
cgtggccgtc ttcttaaccg tcttgctgat cttatcgaac gtgatcgtac ataccttgct 360
gctcttgaaa cacttgataa cggcaaacct tacgttatct cttaccttgt tgatcttgat 420
atggttctta aatgccttcg ttactacgct ggctgggctg ataaatacca tggcaaaaca 480
atccctatcg atggcgattt cttctcttac acacgtcatg aacctgttgg cgtttgcggc 540
caaatcatcc cttggaactt ccctcttctt atgcaagctt ggaaacttgg ccctgctctt 600
gctacaggca acgttgttgt tatgaaagtt gctgaacaaa cacctcttac agctctttac 660
gttgctaacc ttatcaaaga agctggcttc cctcctggcg ttgttaacat cgttcctggc 720
ttcggcccta cagctggcgc tgctatcgct tctcatgaag atgttgataa agttgctttc 780
acaggctcta cagaaatcgg ccgtgttatc caagttgctg ctggctcttc taaccttaaa 840
cgtgttacac ttgaacttgg cggcaaatct cctaacatca tcatgtctga tgctgatatg 900
gattgggctg ttgaacaagc tcatttcgct cttttcttca accaaggcca atgctgctgc 960
gctggctctc gtacattcgt tcaagaagat atctacgatg aattcgttga acgttctgtt 1020
gctcgtgcta aatctcgtgt tgttggcaac cctttcgatt ctaaaacaga acaaggccct 1080
caacttgatg aaacacaatt caaaaaaatc cttggctaca tcaacacagg caaacaagaa 1140
ggcgctaaac ttctttgcgg cggcggcatc gctgctgatc gtggctactt catccaacct 1200
acagttttcg gcgatgttca agatggcatg acaatcgcta aagaagaaat cttcggccct 1260
gttatgcaaa tccttaaatt caaaacaatc gaagaagttg ttggccgtgc taacaactct 1320
acatacggcc ttgctgctgc tgttttcaca aaagatcttg ataaagctaa ctacctttct 1380
caagctcttc aagctggcac agtttgggtt aactgctacg atgttttcgg cgctcaatct 1440
cctttcggcg gctacaaaat gtctggctct ggccgtgaac ttggcgaata cggccttcaa 1500
gcttacacag aagttaaaac agttacagtt aaagttcctc aaaaaaactc t 1551
Claims (10)
1. a kind of acetaldehyde dehydrogenase gene in people source, which is characterized in that its nucleotide sequence is as shown in SEQ ID NO.1.
2. carry the carrier or cell line of gene described in claim 1.
3. a kind of genetic engineering bacterium, which is characterized in that express the bacillus subtilis of acetaldehyde dehydrogenase gene described in claim 1
Bacterium.
4. genetic engineering bacterium according to claim 3, which is characterized in that with bacillus subtilis (Bacillus
Subtilis) 168 be host, and the gene of encoding glyoxylate dehydrogenase is expressed using pMA5 as expression vector.
5. a kind of production method of acetaldehyde dehydrogenase, which is characterized in that engineering bacteria described in claim 1 is seeded to fermentation training
It supports in base, 28~37 DEG C of 12~36h of culture.
6. according to the method described in claim 5, it is characterized in that, the fermentation medium is LB culture mediums.
7. according to the method described in claim 6, it is characterized in that, the fermentation medium also contains Ca2+0.2~0.5g/L.
8. according to the method described in claim 6, it is characterized in that, the fermentation medium also contains Cu2+0.2~0.5g/L.
9. application of the genetic engineering bacterium in the product containing acetaldehyde dehydrogenase is prepared described in claim 3.
10. application of the gene described in claim 1 in alcohol dispelling food or the health products containing acetaldehyde dehydrogenase are prepared.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810148121.9A CN108103081B (en) | 2018-02-13 | 2018-02-13 | Acetaldehyde dehydrogenase and application thereof in anti-alcohol health-care product |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810148121.9A CN108103081B (en) | 2018-02-13 | 2018-02-13 | Acetaldehyde dehydrogenase and application thereof in anti-alcohol health-care product |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108103081A true CN108103081A (en) | 2018-06-01 |
| CN108103081B CN108103081B (en) | 2020-09-04 |
Family
ID=62205758
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810148121.9A Active CN108103081B (en) | 2018-02-13 | 2018-02-13 | Acetaldehyde dehydrogenase and application thereof in anti-alcohol health-care product |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108103081B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110564662A (en) * | 2019-09-30 | 2019-12-13 | 南京农业大学 | Construction method of integrated bacillus subtilis for efficiently expressing acetaldehyde dehydrogenase |
| CN111808877A (en) * | 2020-09-07 | 2020-10-23 | 广州暨南生物医药研究开发基地有限公司 | Production method, composition and preparation of acetaldehyde dehydrogenase |
| US10849938B2 (en) | 2017-09-13 | 2020-12-01 | ZBiotics Company | Gene expression system for probiotic microorganisms |
| CN112143743A (en) * | 2020-09-07 | 2020-12-29 | 广州暨南生物医药研究开发基地有限公司 | Acetaldehyde dehydrogenase gene, escherichia coli engineering bacteria, expression and application |
| CN113046252A (en) * | 2021-03-23 | 2021-06-29 | 江南大学 | Separation and identification of acetaldehyde dehydrogenase high-producing strain |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101091793A (en) * | 2007-06-01 | 2007-12-26 | 复旦大学附属中山医院 | New usage of anti alcoholic drug containing acetadehyde dehydrogenase |
| CN101942465A (en) * | 2010-04-02 | 2011-01-12 | 四川大学 | Method for producing acetaldehyde dehydrogenase of recombinant basophilic salt-tolerant bacillus halodurans (XJU-1) by using genetic engineering technique |
-
2018
- 2018-02-13 CN CN201810148121.9A patent/CN108103081B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101091793A (en) * | 2007-06-01 | 2007-12-26 | 复旦大学附属中山医院 | New usage of anti alcoholic drug containing acetadehyde dehydrogenase |
| CN101942465A (en) * | 2010-04-02 | 2011-01-12 | 四川大学 | Method for producing acetaldehyde dehydrogenase of recombinant basophilic salt-tolerant bacillus halodurans (XJU-1) by using genetic engineering technique |
Non-Patent Citations (2)
| Title |
|---|
| EBERL L. ET AL.: "ALDH2 [Homo sapiens]", 《GENBANK：CAG33272.1》 * |
| 赵绪光 等: "重组嗜碱耐盐芽孢杆菌(XJU-1)乙醛脱氢酶aldA基因在工程菌BL21(DE3)中的表达和酶学性质研究", 《酿酒科技》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10849938B2 (en) | 2017-09-13 | 2020-12-01 | ZBiotics Company | Gene expression system for probiotic microorganisms |
| US11696932B2 (en) | 2017-09-13 | 2023-07-11 | ZBiotics Company | Gene expression system for probiotic microorganisms |
| US11975033B2 (en) | 2017-09-13 | 2024-05-07 | ZBiotics Company | Gene expression system for probiotic microorganisms |
| CN110564662A (en) * | 2019-09-30 | 2019-12-13 | 南京农业大学 | Construction method of integrated bacillus subtilis for efficiently expressing acetaldehyde dehydrogenase |
| CN110564662B (en) * | 2019-09-30 | 2022-03-25 | 南京农业大学 | Construction method of integrated bacillus subtilis for efficiently expressing acetaldehyde dehydrogenase |
| CN111808877A (en) * | 2020-09-07 | 2020-10-23 | 广州暨南生物医药研究开发基地有限公司 | Production method, composition and preparation of acetaldehyde dehydrogenase |
| CN112143743A (en) * | 2020-09-07 | 2020-12-29 | 广州暨南生物医药研究开发基地有限公司 | Acetaldehyde dehydrogenase gene, escherichia coli engineering bacteria, expression and application |
| CN112143743B (en) * | 2020-09-07 | 2021-12-31 | 广州暨南生物医药研究开发基地有限公司 | Acetaldehyde dehydrogenase gene, escherichia coli engineering bacteria, expression and application |
| CN113046252A (en) * | 2021-03-23 | 2021-06-29 | 江南大学 | Separation and identification of acetaldehyde dehydrogenase high-producing strain |
| CN113046252B (en) * | 2021-03-23 | 2022-07-05 | 江南大学 | Separation and identification of acetaldehyde dehydrogenase high-producing strain |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108103081B (en) | 2020-09-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108103081A (en) | A kind of acetaldehyde dehydrogenase and its application in terms of alcohol-neutralize healthy product | |
| Garrity et al. | Class I. Alphaproteobacteria class. nov. | |
| TW200914610A (en) | Isopropyl alcohol-producing bacteria and method for producing isopropyl alcohol using the same | |
| CN103952318B (en) | High yield citric acid waste residue bacterium FY2013 and its application | |
| CN101880696B (en) | Method for producing L-lactic acid by fermentation and bacterial strain using same | |
| CN102994406B (en) | Genetically engineered bacterium for producing glucose oxidase as well as construction and application thereof | |
| CN107201315A (en) | A kind of Paecilomyces thermaphila mutagenic strain and its method of mutagenesis and application | |
| CN107828806A (en) | A kind of β alpha-glucosidase genes of new resistance to glucose and its application | |
| CN107760623B (en) | The A Shi bacillus of the neutral uncooked amylum enzyme of one plant of production | |
| CN106520715A (en) | Short-chain dehydrogenase and gene thereof, recombinant expression vector, genetically engineered bacterium and application thereof in astaxanthin chiral intermediate synthesis | |
| CN103937691B (en) | One plant production β fructosidases aspergillus oryzae strain and its cultural method and application | |
| CN101215533B (en) | Hydantoinase and carbamoyl hydrolase producing strain, bienzyme gene and application thereof for preparing L-amino acid | |
| CN107227284A (en) | A kind of restructuring streptococcus zooepidemicus for the micromolecule hyaluronic acid that ferments | |
| CN102417890B (en) | Sinorhizobium meliloti and method for applying same for fermenting to produce manganese peroxidase | |
| CN105602853A (en) | Bacterial strain of Rasamsonia emersonii and application thereof to production of Pu'er tea | |
| CN102224938B (en) | Application of cold-adapted protease MCP-01 for tenderizing meat | |
| CN106591210B (en) | It is a kind of express vgb gene recombination Corynebacterium glutamicum and its application | |
| CN105802892A (en) | Stenotrophomonas maltophilia for producing keratinase and application of stenotrophomonas maltophilia | |
| CN105861373B (en) | It is a kind of produce keratinase pseudomonas aeruginosa and its application | |
| CN104593306B (en) | A kind of E bacterial strain HY 05C high-density cultivation methods | |
| CN107460152A (en) | Produce recombinant bacterium, construction method and the purposes of rhodioside and the like | |
| CN102936576B (en) | A kind of Xylitol genetic engineering bacterium and mixing thereof transform the method for producing Xylitol | |
| CN108192848A (en) | A kind of Psychrobacter bacterial strain of galactopoiesis carbohydrase and the method that low temperature lactase is prepared using the bacterial strain | |
| CN105950494B (en) | Produce the circle rhodosporidium toruloides and preparation method thereof of linoleic acid plus linolenic acid | |
| CN106754829A (en) | A kind of method of utilization bacillus HS17 fermenting and producing chitosan enzymes and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |