CN102154113B - Penicilliumaurantiogriseum with function of inducing soybeans to generate atrazine resistance and application - Google Patents
Penicilliumaurantiogriseum with function of inducing soybeans to generate atrazine resistance and application Download PDFInfo
- Publication number
- CN102154113B CN102154113B CN2010105930155A CN201010593015A CN102154113B CN 102154113 B CN102154113 B CN 102154113B CN 2010105930155 A CN2010105930155 A CN 2010105930155A CN 201010593015 A CN201010593015 A CN 201010593015A CN 102154113 B CN102154113 B CN 102154113B
- Authority
- CN
- China
- Prior art keywords
- atrazine
- soybean
- snef960
- liquid
- inducing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention relates to a method for culturing penicilliumaurantiogriseum snef960 with a function of inducing soybeans to generate an atrazine resistance after soybean seeds are processed and application of the penicilliumaurantiogriseum snef960, and belongs to the technical field of microbial pesticides. A fungus strain snef960 related to the invention is preserved in the China general microbiological culture collection center on Dec 14th in 2010 and the preservation number of the fungus strain snef960 is CGMCC (China General Microbiological Culture Collection Center) No.4468. The fungus strain snef960 is prepared by liquid fermentation culture; and after the soybean seeds are processed by metabolites of the fungus strain snef960, the soybean seeds can generate the atrazine resistance when sprouting. The soybeans processed by the metabolites of the fungus can be interbred with gramineous crops such as corns and the like. A unified atrazine herbicide is adopted to weed. The difficult problem of weeding of interplanting between the corns and the soybeans is solved. The penicilliumaurantiogriseum snef960 has excellent application and development prospect.
Description
Technical field:
The invention belongs to the microbial pesticide technical field, be specifically related to the tangerine ash penicillium spp and the application of the anti-G-30027 of a kind of tool inducing soybean.
Background technology:
G-30027 is a kind of worldwide widely used dry land herbicide, and Chinese commodity are called atrazine.G-30027 (Atrazine), chemical name 2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazines is the selectivity triazine herbicide, nineteen fifty-two is developed by Switzerland Ciba-Geigy chemical company.Can be used for corn, jowar, sugarcane, fruit tree, nursery, forest land etc. and prevent and kill off annual gramineous weed and broadleaf weeds.Because its good weeding effect and cheap price, nineteen fifty-nine is dropped into commercial prodn.Can remove from office amine with second, alachlor, multiple acetamide-group herbicides such as different third methylamine mix, and strengthen weeding activity and use range.In the dry land herbicide kind, G-30027 occupies critical role.Because cost is low and herbicidal effect good, has obtained large-area popularization and use in countries in the world, be one of important weedicide of Absatzvolumen maximum in the weedicide of selling in the world.The external use in existing 50 years of this weedicide history of using, China brought into use from the beginning of the eighties.
Weedicide has strict selective action to dicotyledonous with monocot crops, and the weedicide big area is used, and makes the good cultivation step of crop intercropping and interplanting be difficult to realize.Particularly more outstanding to the intercropping and interplanting performance of corn and soybean.Usually corn field will adopt the G-30027 weedicide to carry out the preceding sealing of seedling weeding, and G-30027 has stronger toxic action to soybean, and therefore, mostly the producer is to adopt clear corn or the employing artificial weeding of planting.For the soybean field, weeding is difficulty comparatively, and the weedicide cost in soybean field is higher relatively, uses weedicide to be prone to produce poisoning.
There is this problem to producing to go up; Obtain a fungal strain through screening system; Utilize the meta-bolites of this bacterium or this bacterium to handle soybean seeds, but inducing soybean produces the resistance to herbicide atrazine at seed germination and growing period, thereby solved the problem of soybean field with the G-30027 weeding
Summary of the invention:
But the object of the invention inducing soybean produces the bacterial strain to the herbicide atrazine resistance at seed germination and growing period; After bacterial strain pressed the conventional liq fermentation culture; Can use the meta-bolites of this bacterium or this bacterium; Develop the seed treatment agent of handling soybean seeds, treated soybean seeds can make soybean produce the resistance to the responsive G-30027 weedicide of soybean.
The fungus strains that the present invention screens acquisition is tangerine ash mould (Penicillium aurantiogriseum) snef960, depositary institution: China Committee for Culture Collection of Microorganisms common micro-organisms center; Address: China. No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City; Preservation date: on December 14th, 2010; The numbering that preservation is registered on the books: CGMCC NO.4468.Tangerine ash mould (Penicillium aurantiogriseum) snef960 is the strain that from Chinese ShenYang, Liaoning Province city Agricultural University Of Shenyang experimental plot, filters out has strong induced activity to soybean a fungal bacterial strain.
The present invention is through discovering; Soil fungi has a lot of fungal strains all to have certain activity, from these fungies, filter out a strain proterties relatively preferably bacterial strain tangerine ash mould (Penicillium aurantiogriseum) snef960 carried out further research.
The invention still further relates to the fungal metabolite that is used for the inducing soybean seed, it is to extract preparation after the liquid fermentation and culture by fungal bacterial strain tangerine ash mould (Penicillium aurantiogriseum) snef960 process routine of the present invention.
Fungal bacterial strain tangerine ash mould (Penicillium aurantiogriseum) snef960 of the present invention after cultivating through the test tube kind, prepares through enlarging fermentation culture again, and its concrete grammar is following:
1. the test tube kind is cultivated
Culture medium prescription is potato dextrose agar (PDA): yam (peeling) 200g, glucose (or sucrose) 10g, agar 17-20g, water 1000mL.
2. liquid fermentation and culture
Wherein the liquid fermentation medium prescription is: by weight percentage, and NaNO
30.1%-0.3%, KH
2PO
40.1%-0.2%, KCl 0.03%-0.8%, MgSO
47H
2O 0.05%-0.1%, FeSO
40.001%-0.002%, sucrose 2%-3%, agar 1.5%-2%, remainder are zero(ppm) water, pH is 7-9.
The test tube kind is inoculated in 250mL triangular flask (the every bottled 100mL) liquid nutrient medium, and under 25 ℃～28 ℃, shaking speed is with 150rmin
-1～180rmin
-1, fermentation 6-9d, preferred 9d.
The bacterial classification of liquid fermenting can carry out scale prodn through large fermentation tank, and its product is the finished product of fermented liquid or fermented liquid.
The invention still further relates to a kind of soybean seeds treatment agent, its tangerine of the present invention ash mould (Penicilliumaurantiogriseum) snef960 itself or its metabolite that comprises significant quantity is as the effective active composition.
Tangerine ash mould (Penicillium aurantiogriseum) snef960 of the present invention or its metabolite have the induced activity to soybean; The seed treatment that can be used for soybean; Soybean after the processing can be adopted the responsive atrazine of soybean is carried out weeding, thereby solves the problem of soybean field with the G-30027 weeding.
Embodiment:
In order further to illustrate in more detail rather than, to have provided the following example in order to limit the present invention.
Embodiment 1: the cultivation of tangerine ash mould (Penicillium aurantiogriseum) snef960
At first tangerine ash mould (Penicillium aurantiogriseum) snef960 bacterial strain is carried out fermentation culture, then the meta-bolites after the fermentation culture is carried out the test of soybean induced activity, confirm its inducing action soybean.
The thalline of tangerine ash mould (Penicillium aurantiogriseum) snef960 is inoculated on the test tube nutrient agar; Culture medium prescription is PDA substratum, i.e. composition: 7 yams (peeling) 200g, glucose (or sucrose) 10g; Agar 17-20g adds water to 1000mL.Cultivate 7d for 25 ℃, obtain the test tube kind.
The test tube kind is inoculated in 250mL triangular flask (the every bottled 100mL) liquid nutrient medium, culture medium prescription is again: by weight percentage, and NaNO
30.2%, K
2HPO
40.1%, MgSO
4.7H
2O 0.05%, and KCL 0.05%, FeSO
4.7H
2O 0.001%, and sucrose 3%, adding distil water are to 1000mL, and pH is 7.Cultivate down at 25 ℃, shaking speed is with 180rmin
-1, the fermentation 9d, the fermented liquid of formation or the finished product of fermented liquid can directly or indirectly be handled soybean seeds.The soybean seeds that is processed can be planted in the soil that sprays atrazine herbicidal, can resist the poisoning of G-30027 (atrazine) when soybean emerges.
Embodiment 2: the fermentation of tangerine ash mould (Penicillium aurantiogriseum) snef960
Basically with embodiment one, difference is that the bacterial classification inoculation of the liquid culture fermentor tank to 1000L is fermented, and cultivates down at 25 ℃, and pH is 7, fermentation 7d.
Embodiment 3: the use of tangerine ash mould (Penicillium aurantiogriseum) snef960 leavened prod
Use fermented liquid stoste,, after drying, the soybean seeds of handling is normally sowed, press 350g/667m in 1: 50 ratio treatment coatings soybean seeds
2Spray G-30027 (atrazine), the soybean growth of can normally emerging.
Claims (7)
1. a strain has the fungal bacterial strain that inducing soybean produces anti-G-30027 (atrazine) resistance after handling soybean seeds; It is characterized in that: this bacterial strain is a tangerine ash mould (Penicillium aurantiogriseum); Be deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center on December 14th, 2010, preserving number is CGMCC NO.4468.
2. the liquid fermentation process of the described fungal bacterial strain of claim 1, it is characterized in that: the liquid fermentation medium prescription is: by weight percentage, NaNO
30.2%-0.3%, KH
2PO
40.1%-0.2%, KCl 0.05%-0.1%, MgSO
47H
2O0.05%-0.1%, FeSO
40.001%-0.002%, sucrose 2%-3%, remainder is a zero(ppm) water, and pH is 7-9, and leavening temperature is 25-28 ℃, shaking speed 150rmin
-1～180rmin
-1, fermentation time 6-9d.
3. liquid fermentation process according to claim 2 is characterized in that: described fermentation time is 9d.
4. has the preparation method that inducing soybean produces the fermented liquid stoste of anti-G-30027 (atrazine) resistance; It is characterized in that: form through behind the liquid fermenting by the described fungal bacterial strain of claim 1; The liquid fermentation medium prescription is: by weight percentage, and NaNO
30.2%-0.3%, KH
2PO
40.1%-0.2%, KCl 0.05%-0.1%, MgSO
47H
2O0.05%-0.1%, FeSO
40.001%-0.002%, sucrose 2%-3%, remainder is a zero(ppm) water, and pH is 7-9, and leavening temperature is 25-28 ℃, shaking speed 150rmin
-1～180rmin
-1, fermentation time 6-9d.
5. preparation method according to claim 4 is characterized in that: described fermentation time is 9d.
6. a soybean seeds treatment agent is characterized in that, comprises formed fermented liquid stoste in the liquid fermentation process according to claim 2 of significant quantity, is used for inducing soybean and produces the resistance to the responsive herbicide atrazine (atrazine) of soybean.
7. the application of the fermented liquid stoste that forms in the said liquid fermentation process of claim 2 in the anti-G-30027 of inducing soybean (atrazine) weedicide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105930155A CN102154113B (en) | 2010-12-17 | 2010-12-17 | Penicilliumaurantiogriseum with function of inducing soybeans to generate atrazine resistance and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105930155A CN102154113B (en) | 2010-12-17 | 2010-12-17 | Penicilliumaurantiogriseum with function of inducing soybeans to generate atrazine resistance and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102154113A CN102154113A (en) | 2011-08-17 |
| CN102154113B true CN102154113B (en) | 2012-11-07 |
Family
ID=44435789
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105930155A Expired - Fee Related CN102154113B (en) | 2010-12-17 | 2010-12-17 | Penicilliumaurantiogriseum with function of inducing soybeans to generate atrazine resistance and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102154113B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106417290A (en) * | 2016-09-26 | 2017-02-22 | 哈尔滨众诚农业科技有限公司 | Fomesafen antidote preparation method |
| CN110122218A (en) * | 2019-05-31 | 2019-08-16 | 甘肃农业大学 | A kind of N application method balancing corn intercrop pea nitrogen supply and demand relationship |
-
2010
- 2010-12-17 CN CN2010105930155A patent/CN102154113B/en not_active Expired - Fee Related
Non-Patent Citations (6)
| Title |
|---|
| 3种霉菌发酵液对作物的促长作用初报;李明等;《中国农学通报》;20100510;第26卷(第9期);第299-302页 * |
| 叶新强等.除草剂阿特拉津的使用与危害.《环境科学与管理》.2006,第31卷(第8期),第95-97页. |
| 朱晓峰等.黑曲霉发酵液中有机酸的分析及对植物线虫的影响.《农药》.2009,第48卷(第2期),第137-139页. |
| 李明等.3种霉菌发酵液对作物的促长作用初报.《中国农学通报》.2010,第26卷(第9期),299-302. |
| 除草剂阿特拉津的使用与危害;叶新强等;《环境科学与管理》;20061231;第31卷(第8期);第95-97页 * |
| 黑曲霉发酵液中有机酸的分析及对植物线虫的影响;朱晓峰等;《农药》;20090228;第48卷(第2期);第137页右栏"1.1.3 发酵液的制备"节 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102154113A (en) | 2011-08-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104017744B (en) | The preparation method of the Pseudomonas chlororaphis agent of disease-resistant growth-promoting and purposes | |
| CN103451135B (en) | Bacillus subtilis M3 and application thereof | |
| CN101445785B (en) | Aspergillus niger fungus capable of poisoning plant parasitic nematodes, preparation method and application thereof | |
| CN106497831B (en) | A kind of prevention and treatment Phytophthora nicotianae disease composite bacteria agent and its preparation method and application | |
| CN104894035A (en) | Bacillus screening method and application thereof | |
| CN1994092A (en) | A biological weed killer and preparation process thereof | |
| Zhao et al. | Coordinated effects of root autotoxic substances and Fusarium oxysporum Schl. f. sp. fragariae on the growth and replant disease of strawberry | |
| CN104877950B (en) | The bacillus pumilus of one high-efficiency degradation Atrazine | |
| CN103074272A (en) | Bacillus methylotrophicus Sanju-04 and its application | |
| CN102911875A (en) | Preparation method for multifunctional soil remediation microbial inoculum | |
| CN102108339B (en) | Bacillus megaterium with capability of inducing stress resistance of soybeans and application thereof | |
| CN105462881A (en) | Paenibacillus polymyxa for preventing and treating vertieillium wilt in crops and application of paenibacillus polymyxa | |
| CN102154157A (en) | Bio-control strain 4AT8 capable of preventing and curing various bacterial diseases | |
| CN104805019A (en) | Endophytic fungus capable of promoting nutrient element absorption of wood oil tree | |
| CN102154114B (en) | Penicillium Chrysogenun inducing meloidogyne incognita chitwood resistance in tomatoes and use thereof | |
| CN111733081A (en) | Biological agent formula suitable for preventing and treating tomato root-knot nematode diseases | |
| CN105532411A (en) | Method for storing arbuscular mycorrhizal fungi | |
| CN102108338B (en) | Cold-resistance-inducing Pseudomonas aeruginosa strain and application thereof | |
| CN109748735A (en) | A kind of method of the quick decomposition of green plant stalk and prevention and control flue-cured tobacco root knot nematode disease | |
| CN102154113B (en) | Penicilliumaurantiogriseum with function of inducing soybeans to generate atrazine resistance and application | |
| CN105112325A (en) | Bacillus cereus bacterial strain WL08 for efficiently degrading dimethomorph, and application and using method of bacillus cereus bacterial strain | |
| CN104630072A (en) | Trichoderma atroviride Ta-9 strain and application thereof in prevention and control of rice diseases | |
| CN101831395B (en) | Klebsiella pneumoniae for inducing soybean to resist atrazine and application | |
| CN101818124B (en) | Stenotrophomonas capable of improving herbicide resistance in soybeans, method for preparing same and application thereof | |
| CN104195076A (en) | Bacillus methylotrophicus Sanju-04 and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121107 Termination date: 20211217 |