CN102154093B - Plate type photobioreactor - Google Patents
Plate type photobioreactor Download PDFInfo
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- CN102154093B CN102154093B CN 201110006738 CN201110006738A CN102154093B CN 102154093 B CN102154093 B CN 102154093B CN 201110006738 CN201110006738 CN 201110006738 CN 201110006738 A CN201110006738 A CN 201110006738A CN 102154093 B CN102154093 B CN 102154093B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
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Abstract
The invention discloses a plate type photobioreactor. The plate type photobioreactor comprises a plurality of independent reactor units which are connected in series and mutually communicated, wherein adjacent reactor units are connected with each other by a tie bar assembly; each reactor unit is formed by bonding a transparent front plate, a back plate, side plates and a base plate so as to create a bioreaction space; the tie bar assembly comprises a front tie bar support plate, a back tie bar support plate and a built-in tie bar; one end of the built-in tie bar is fixed with the front tie bar support plate, and the other end of the built-in tie bar is fixed with the back tie bar support plate; and for the adjacent reactor units, the front plate parts are bonded to one side of the front tie bar support plate, the back plate parts are bonded to one side of the back tie bar support plate, and the side plates are respectively bonded to one side of the built-in tie bar. By using the built-in tie bar assembly to tightly connect the front and back plates of each reactor unit together, the supporting capacity of the reactor is improved, thus the photobioreactor is applicable to industrial-level culture.
Description
Technical field
The present invention relates to photobioreactor field, relate in particular to a kind of flat bioreactor that wraps up built-in lacing wire component.
Background technology
Little algae is to live in multipotency in fresh water or briny environment, large to carry out photosynthetic lower plant.Little algae is generally unicellular, and its individuality is less, need to could observe its morphological structure by microscope.Single microalgae cell is equivalent to the plant of a higher plant, and it can constantly breed and exercise diversified physiology, biochemical function.These single celled little algaes can be by being similar to culture apparatus microbial fermentation, can transmitted light---and bioreactor carries out mass-producing and cultivates.Therefore bioreactor should be a kind of key equipment of realizing that efficiently, fast mass-producing is cultivated, can obtain higher biomass by it, and then refines further the dissimilar little algae biological products of acquisition.
Mainly divide open and closed two large types for the bioreactor of cultivating little algae.The characteristics of Race-way photobioreactor are: simple in structure; Easily amplify; Cost is low; Specific surface area is little; The efficiency of light energy utilization is low; To the more difficult control of biological parameter; Easily pollute; Can't realize sterile culture; Production efficiency is lower; And CO
2Utilization ratio is low.The characteristics of closed photo bioreactor are: the structure relative complex; Be subject to certain limitation during amplification; Specific surface area is large; The efficiency of light energy utilization is high; Compare with Race-way photobioreactor, growth parameter(s) is easily controlled; Easily control and pollute; Can realize sterile culture under given conditions; Production efficiency is higher; CO
2Utilization ratio is higher.
The closed photo bioreactor that mostly uses at present mainly contains three types: pipeline reactor, column reactor and flat-plate reactor.Pipeline reactor oxygen is resolved difficulty, and easy cleaning not too be fit to the large scale culturing of little algae, and cost is high.Pillar bioreactor unit volume is less, be difficult for to amplify, production efficiency is not high yet.Flat bioreactor has following characteristics: light-receiving area and volume of culture large percentage; Being suitable for outdoor little algae cultivates; Optical path can be regulated; Optical energy utilization efficiency is high; Can obtain higher biomass; Cost is lower; Easily clean; Easily control; The cumulative concentration of oxygen is low.Therefore, this flat bioreactor is paid close attention to widely in the cultivation of the little algae of photoautotrophy type.As far back as nineteen fifty-three, Milner just begins to utilize the transparent vessel of rectangle to cultivate little algae, for the application of dull and stereotyped reactor is laid a good foundation.After this, Samson and Leduy have invented a kind of flat bioreactor, and have been equipped with luminescent lamp as light source (1985).After 1 year, Ramos de Ortega and Roux have invented a kind of outdoor dull and stereotyped reactor that can be used for, and it is (1986) that make of thick transparent PVC material.After this, many bibliographical informations the design of dissimilar vertical flat plate bioreactor, and be applied to different types of micro-algae large-scale cultivate in (Tredici﹠amp; Materassi, 1992; Hu et al., 1996; Zhang et al., 2002; Hoekema et al., 2002).
The existing flat-plate reactor that is suitable for the large scale culturing of little algae cultivation generally is comprised of transparent dull and stereotyped casing.The material that casing adopts is silicate glass or synthetic glass, at box house, adopts the dividing plate of casing same material to stick between the flat board of front and back, thereby plays certain reinforcement effect.In addition, the outside bonding of the reactor that also has strip of paper used for sealing is with further reinforcing.Yet, this transparent vessel that is bonded by some flat boards and acid glass cement, keeping the stable bounding force of structure of reactor provides by acid glass cement, has larger defective.When the micro algae culturing liquid volume is large, when liquid height raises, its strengthens the pressure of reactor wall, the glass cement that at this moment very easily causes the front and rear panel bonding part of internal partition and reactor takes off and splits, and finally causes whole reactor to break fully.If when adopting synthetic glass as tank material and with the synthetic glass glue bond, because having plasticity, synthetic glass easily causes the poly (methyl methacrylate) plate distortion, finally cause whole reactor break.For these reasons, when no matter the flat bioreactor that designs is applied in laboratory study or is applied in scale operation, except the amplification performance that will consider it, processing property, culture efficiency, also must consider its weight capacity, wearing quality, work-ing life and aesthetic measure.
At present, published flat bioreactor patent documentation and be used for the flat bioreactor that micro-algae large-scale cultivates and mainly have following shortcoming:
(1) classic flat-plate formula bioreactor is by the transparent vessel that some sheet glass and acid glass cement are bonded, and keeping the stable bounding force of structure of reactor provides by acid glass cement.On the one hand, the quality of glass cement, life-span and bonding skill can directly affect the stability of reactor; On the other hand, the pulling force that glass cement provides is limited, increase along with height for reactor and volume, liquid can strengthen the pressure of front and back main body sheet glass, cause acid glass cement bonding separation sheet glass (glass lacing wire) and front and back main body sheet glass securely, can cause breaking of flat bioreactor.
(2) for guaranteeing the stability of flat bioreactor structure, usually in the bonding some glass lacing wires of inside reactor.But the shortcoming of glass lacing wire maximum is that fragility is larger, in case slight slight crack appears in the glass lacing wire or the installation site is unreasonable, the device that all can induce reaction breaks.
(3) the flat bioreactor of present published external reinforced formula, external reinforcement is more with material, and aesthetics is relatively poor, and these materials have also blocked a part of light and enter photoreactor.
(4) existing bonding glass technology, the flat bioreactor after being difficult to guarantee to amplify uses for a long time.
Summary of the invention
In view of this, the purpose of this invention is to provide a kind of flat bioreactor that can be used for little algae large scale culturing, classic flat-plate formula bioreactor is difficult to realize large volume culture, structural stability is poor, work-ing life is short and keep in repair the shortcomings such as loaded down with trivial details in order to solve.
For solving the problems of the technologies described above, technical scheme of the present invention is: a kind of flat bioreactor, and the separate reactors unit that comprises a plurality of series connection and be interconnected, and connect by a lacing wire component between the adjacent reactor unit; Described reactor unit is bonded by transparent front plate, rear plate, side plate and base plate, forms a biological reaction compartment; Described lacing wire component comprises the front fagging of a lacing wire, a lacing wire rear stay plate and a built-in lacing bar, and before an end of this built-in lacing bar and described lacing wire, fagging is fixed, and the other end and described lacing wire rear stay plate are fixed; The former board part of adjacent reactor unit is bonded in the same side of the front fagging of described lacing wire, and rear plate portion is bonded in the same side of described lacing wire rear stay plate, and side plate is bonded in respectively a side of described built-in lacing bar.
More preferably, before described lacing wire, fagging, lacing wire rear stay plate and built-in lacing bar are flat board; Before one end of described built-in lacing bar and described lacing wire, fagging is fixing, and the other end and described lacing wire rear stay plate are fixed.
More preferably, before described lacing wire, fagging and lacing wire rear stay plate are flat board, and described built-in lacing bar is the flat tube of a top and bottom sealing; Offer some groups of through holes on plate after the front fagging of described lacing wire, flat tube and lacing wire, run through a locking member in every group of through hole.
More preferably, also comprise before lacing wire stiffening plate after stiffening plate and lacing wire; After described lacing wire front end panel, the front stiffening plate of lacing wire, lacing wire rear bearing sheet and lacing wire, stiffening plate is flat board, and described built-in lacing bar is some pipes; Before one end of described pipe and described lacing wire, fagging is fixed, and the other end and described lacing wire rear stay plate are fixed; After the front stiffening plate of described lacing wire and lacing wire, stiffening plate is offered the some group through holes corresponding with described pipe, runs through a locking member in every group of through hole and corresponding pipe.
More preferably, before described lacing wire, fagging comprises the first " L " template and second " L " template, and described lacing wire rear stay plate and described built-in lacing bar are respectively base plate and the intermediate plate of T-shape plate; Offer some groups of through holes on intermediate plate and second " L " template of described first " L " template, T-shape plate, run through a locking member in every group of through hole.
More preferably, comprise the reactor kerve, be provided with groove on described reactor kerve, the two side of described groove touch respectively header board and the rear plate of described reactor unit.
More preferably, described reactor kerve is provided with cushion, between the diapire of the base plate of described reactor unit and described groove.
More preferably, described groove is provided with some discharge gates, and each discharge gate is communicated with a reactor unit.
More preferably, described discharge gate comprises drainage conduit and is installed on valve on described drainage conduit, and described drainage conduit is passed the base plate of described groove and described reactor unit.
More preferably, the upper edge of described drainage conduit is lower than the base plate of described reactor unit or concordant with the base plate of described reactor unit.
Compared with prior art, the present invention is in the inner packaging type lacing wire component ruggedized construction of introducing various ways of flat bioreactor, can closely link together the forward and backward plate of each reactor unit, thereby greatly improved the supporting capacity of reactor, made reactor have advantages of that structural stability is good, long service life and easy to maintenance.This structure of reactor is simple, durable, and the liquid of the top and the bottom in whole reactor assembly can be communicated with mobile, and easily is amplified to other culture system of industrialization level.In addition, lacing wire ruggedized construction of the present invention can not shut out the light, and does not affect good looking appearance yet.
Description of drawings
Fig. 1 is the schematic diagram of the flat bio-reactor of the present invention;
Fig. 2 A is the frontview of lacing wire component embodiment one in Fig. 1;
Fig. 2 B is the left view of lacing wire component embodiment one in Fig. 1;
Fig. 2 C is the vertical view of lacing wire component embodiment one in Fig. 1;
Fig. 2 D is the stereographic map of lacing wire component embodiment one in Fig. 1;
Fig. 3 A is the frontview of lacing wire component embodiment two in Fig. 1;
Fig. 3 B is the left view of lacing wire component embodiment two in Fig. 1;
Fig. 3 C is the vertical view of lacing wire component embodiment two in Fig. 1;
Fig. 3 D is the stereographic map of lacing wire component embodiment two in Fig. 1;
Fig. 4 A is the frontview of lacing wire component embodiment three in Fig. 1;
Fig. 4 B is the left view of lacing wire component embodiment three in Fig. 1;
Fig. 4 C is the vertical view of lacing wire component embodiment three in Fig. 1;
Fig. 4 D is the stereographic map of lacing wire component embodiment three in Fig. 1;
Fig. 5 A is the frontview of lacing wire component embodiment four in Fig. 1;
Fig. 5 B is the left view of lacing wire component embodiment four in Fig. 1;
Fig. 5 C is the vertical view of lacing wire component embodiment four in Fig. 1;
Fig. 5 D is the stereographic map of lacing wire component embodiment four in Fig. 1;
Fig. 6 is the design sketch after flat bio-reactor assembling shown in Figure 1 is completed.
Embodiment
In order to make those skilled in the art understand better technical scheme of the present invention, the present invention is described in further detail below in conjunction with the drawings and specific embodiments.
Referring to Fig. 1, be a preferred embodiment of the flat bioreactor of the present invention, it comprises: then a glass plate by the specific standard size is bonded together them with glass cement and a closed transparent reactor assembly 1 forming; Some built-in stainless steel lacing wire components 2; One stainless steel reactor kerve 3.
Described closed transparent reactor assembly 1 is the main part of flat bioreactor, and little algae receives luminous energy and various function nutrition composition therein, thereby carries out the cell fission growth.This closed transparent reactor assembly 1 is comprised of some independences and the reactor unit 11 that is interconnected, wherein: each reactor unit 11 comprises a glass film plates 111, a glass front plate 113, a back glass 114 and two blocks of glass side plates 112, bonding by acid glass cement between them, form a biological reaction compartment; Adjacent reactor unit 11 connects by built-in stainless steel lacing wire component 2, but the plural serial stage of realization response device unit 11 and being interconnected, thus form a larger reaction system.In the present embodiment, adjacent reactor unit 11 is communicated with by the communicating aperture of glass side plate top and bottom, thereby guarantees circulating of nutrient solution in whole flat bioreactor.Usually, according to the requirement of volume of culture, can select the quantity of suitable glasses plate thickness, height and reactor unit 11, not repeat them here.
The built-in stainless steel lacing wire component 2 of described reactor is the core components that maintain the stable and realization response device of reactor unit 11 plural serial stages, and it comprises fagging before a lacing wire, a lacing wire rear stay plate and a built-in lacing bar.Before one end of described built-in lacing bar and lacing wire, fagging is fixed, and the other end and lacing wire rear stay plate are fixed.During assembling, the former board part of adjacent reactor unit is bonded in the same side of fagging before lacing wire, and rear plate portion is bonded in the same side of lacing wire rear stay plate, and side plate is bonded in respectively a side of built-in lacing bar.Like this, apply by 2 pairs of forward and backward sheet glass of lacing wire component the stability that certain pulling force is kept structure of reactor, can overcome to a great extent the shortcoming that traditional reactor is only brought as interior lacing wire by sheet glass.
Described reactor kerve 3 is the device for reinforcing bottom of each reactor unit 11, protects flat bioreactor bottom glass plate not damaged by the hard bottom surface.This reactor kerve 3 comprises stainless steel groove 31, transparent plastics cushion (scheming not shown) and discharge gate 32 3 parts, wherein: the two side of stainless steel groove 31 touch respectively the forward and backward plate of reactor unit 11, increased on the one hand the anti-damage capability of reactor bottom, also the forward and backward plate of reactor is applied anchorage force on the other hand, stop reactor unit to be split by the bottom; The transparent plastics cushion is placed between stainless steel groove 31 diapires and reactor base plate, plays preferably shock absorption; Discharge gate 32 is arranged on an end of stainless steel groove 31, formed by a stainless steel circle drainage conduit and a valve, this stainless steel circle drainage conduit is passed stainless steel groove 31 and reactor bottom sheet glass, preferably with the upper edge of stainless steel circle drainage conduit slightly lower than or concordant with the reactor bottom sheet glass, thorough to guarantee discharge.Certainly, also a plurality of discharge gates can be set on stainless steel groove 31, each discharge gate is communicated with a reactor unit, does not repeat them here.
Embodiment one
Simultaneously referring to Fig. 2 A～Fig. 2 D, the lacing wire component of the first type is " barrier type ", formed by fagging 21, stainless steel built-in lacing bar dividing plate 22 and stainless steel lacing wire rear stay plate 23 before the stainless steel lacing wire, wherein: lacing wire dividing plate 22 is built-in lacing bar, the middle part of fagging 21 welding before one end and lacing wire, the middle part welding of the other end and lacing wire rear stay plate 23, thus be welded into " work " font structure by these three stainless steel plates.
During assembling, before lacing wire, the header board of adjacent reactor unit is pushed down in the inboard of fagging 21, and the rear plate of adjacent reactor unit is pushed down in the inboard of lacing wire rear stay plate 23; According to reactor optical path size, determine the width of middle part stainless steel lacing wire dividing plate 22, and make the side plate of adjacent reactor unit be bonded in respectively the both sides of the stainless steel lacing wire dividing plate 22 at " work " font structure middle part.
Embodiment two
Simultaneously referring to Fig. 3 A～Fig. 3 D, the lacing wire component of the second type be " stainless steel hollow template ", by sealing and the stainless steel lacing wire flat tube 24 of hollow and rivet 29 etc. form in fagging 21, stainless steel lacing wire rear stay plate 23, top and bottom before the stainless steel lacing wire.Wherein, open some groups of identical through holes of even distribution and position on fagging 21, stainless steel lacing wire rear stay plate 23 before stainless steel lacing wire flat tube 24 and stainless steel lacing wire; Rivet runs through these through holes as locking member, so that fixing corresponding reactor unit.
During assembling, before the stainless steel lacing wire, the both sides of fagging 21 partly are pressed in and are bonded on the header board of adjacent reactor unit respectively, the both sides of stainless steel plate lacing wire rear stay plate 23 part respectively are pressed in and are bonded on the rear plate of adjacent reactor unit, and the side plate of adjacent reactor unit is bonded in respectively the both sides of stainless steel lacing wire flat tube 24, at last by nut and rivet to the stainless steel lacing wire before fagging 21 and 23 reinforcement effects of stainless steel lacing wire rear stay plate.
The advantage of this " stainless steel hollow template " lacing wire component structure is easy accessibility, breaks down as certain reactor unit, and quick release keeps in repair.
Embodiment three
Simultaneously referring to Fig. 4 A～Fig. 4 D, this lacing wire component is " abacus-type ", is comprised of four stainless steel plates and some hollow stainless steel lacing wire pipes 25 and rivet.These four stainless steel plates are respectively stiffening plate (scheming not shown) after the front fagging 21 of stainless steel lacing wire, stainless steel lacing wire rear stay plate 23, the front stiffening plate of stainless steel lacing wire (scheming not shown) and stainless steel lacing wire, wherein: stainless steel lacing wire pipe 25 welds together with the front fagging 21 of stainless steel lacing wire and stainless steel lacing wire rear stay plate 23, forms " abacus " structure.And after the front stiffening plate of lacing wire and lacing wire, stiffening plate is offered the some group through holes corresponding with pipe 25, runs through a locking member in every group of through hole and corresponding pipe 25.
During assembling, the forward and backward plate of reactor unit contracts out this " abacus " structure, and rivet 29 passes the through hole of offering on stiffening plate after stiffening plate and lacing wire before stainless steel lacing wire pipe 25, lacing wire as locking member, in order to reinforce corresponding reactor unit.
Embodiment four
Simultaneously referring to Fig. 5 A～Fig. 5 D, this lacing wire component is " zipper form ", similar zipper, formed by first " L " type stainless steel plate 26, second " L " type stainless steel plate 27, T-shaped stainless steel plate 28 and some rivets 29 and rubber pad, wherein: offer some through holes on the intermediate plate of first " L " type stainless steel plate 26, second " L " type stainless steel plate 27, T-shaped stainless steel plate 28 templates, rivet passes these through holes, with the forward and backward plate of fixing adjacent reactor unit.
Embodiment five
This type lacing wire component is " plastic hollow template ", the structure two of its structure and embodiment two described lacing wire components is similar, difference is, stainless steel lacing wire flat tube 24 is replaced to the lacing wire flat tube configuration that acrylic material and glass material combine, and do not repeat them here.
Adopt above-mentioned arbitrary stainless steel lacing wire component 2 as the interconnecting piece of reactor unit 11, but equal plural serial stages of realization response device unit.In addition, the introducing of this lacing wire component structure has improved the anti-damage capability of reactor, has only overcome by sheet glass as the disadvantage that interior lacing wire brings, and has increased the work-ing life of reactor.In addition, in reactor unit, nutrient solution all is communicated with by connecting passage, has greatly increased the flow circuit of nutrient solution, can play promoter action to the growth of little algae.
Below the specific embodiments of the present invention's one test subject is described:
1, the structure project of the flat bioreactor of pilot scale
Building volume of culture is the flat bioreactor system of 1000L left and right, in order to the cultivation of oil-producing microalgae pilot scale.Consider the restriction of oil-producing microalgae oil and fat accumulation characteristic and culture space, select the less and highly higher reactor of optical path, thereby improve grease harvest yield and reduce the reactor floor space.In this enforcement project arranges, flat bioreactor optical path 3cm, each reactor unit size 120cm * 60cm * 3cm (maximum dress liquid liquid level is 100cm), can be calculated 55 reactor units, each reactor unit volume of culture is 18L, consider to peel off the ability to bear of liquid pressure and the cost of glass, selecting thickness is the simple glass of 12mm, the reactor lacing wire component can be selected any one according to practical situation, this project is selected the second type, and concrete implementation step is:
(1) at first utilize comparatively cheap cement or angle bar to make reactor frame, can be arranged on indoorly, also can be arranged on outdoor;
(2) make the reactor kerve, be of a size of 5cm * 3cm * 3300cm, (every section is that 5cm * 3cm * 3300cm), the reactor kerve arranges a discharge gate every 10m in reactor kerve sectional processing welding;
(3) the reactor kerve is arranged on reactor frame, after water level gauge conditioned reaction device kerve level, with glass cement, the plastic, transparent cushion is adhered in the reactor kerve, the reactor glass film plates is adhered on transparent cushion, completes reactor bottom and makes;
(4) lacing wire component is welded to the specified location of reactor stainless steel kerve, every one of 60cm welding;
(5) at first reactor unit side glass plate is adhered in the stainless steel lacing wire, then after the conglutination reaction device, the main body sheet glass arrives and formulates the position, after the band glass cement is done, the main body sheet glass is to specified location before the conglutination reaction device, after screw rod passes two stainless steel plates and stainless steel flat tube, two of front and back main body sheet glass is firmly fixing, and as shown in Figure 6, flat photoreactor main part completes;
(6) in culturing process, required light irradiation apparatus, circulation cooling device and aeration equipment need configuration in addition.
2, the high-density culture of little algae test
Test the high 120cm of flat bioreactor used, wide 120cm, optical path 3.3 cm, long-pending 36 L of dress liquid.During use, according to the growth needs of different algae kinds, add fresh culture to carry out the cultivation of little algae in reactor.
Adopt above-mentioned flat bioreactor successfully to carry out the cultivation of produce oil chlorella and grid algae, culture condition is as follows: light intensity 300 μ molm
-2S
-1, 24 ℃ ± 1 ℃ of temperature, cultivate and can reach harvesting time after 16 days, dry cell weight can reach 5-6gL
-1, in cell, total lipid content can reach 40～50% of dry weight.
Above-mentioned reactor has used more than 1 year, and running condition is good.
In above embodiment, the material of reactor unit is glass, also can adopt other transparent material; The material of lacing wire component and reactor kerve is stainless steel, obviously also can other metal or the material such as plastic cement.Focus in the present invention is the structure of reactor, it is made material section be restricted, and the user can be selected as the case may be, does not repeat them here.
Above the present invention is described in detail, uses specific case in literary composition principle of the present invention and embodiment are set forth, wherein the explanation of above embodiment just is used for helping understanding method of the present invention and core concept thereof.Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention, can also carry out some improvement and modification to the present invention, these improvement and modification also fall in the protection domain of claim of the present invention.
Claims (10)
1. a flat bioreactor, is characterized in that, the separate reactors unit that comprises a plurality of series connection and be interconnected, and connect by a lacing wire component between the adjacent reactor unit; Described reactor unit is bonded by transparent front plate, rear plate, side plate and base plate, forms a biological reaction compartment; Described lacing wire component comprises the front fagging of a lacing wire, a lacing wire rear stay plate and a built-in lacing bar, and before an end of this built-in lacing bar and described lacing wire, fagging is fixed, and the other end and described lacing wire rear stay plate are fixed; The former board part of adjacent reactor unit is bonded in the same side of the front fagging of described lacing wire, and rear plate portion is bonded in the same side of described lacing wire rear stay plate, and side plate is bonded in respectively a side of described built-in lacing bar.
2. flat bioreactor as claimed in claim 1, is characterized in that, before described lacing wire, fagging, lacing wire rear stay plate and built-in lacing bar are flat board; Before one end of described built-in lacing bar and described lacing wire, fagging is fixed, and the other end and described lacing wire rear stay plate are fixed.
3. flat bioreactor as claimed in claim 1, is characterized in that, before described lacing wire, fagging and lacing wire rear stay plate are flat board, and described built-in lacing bar is the flat tube of a top and bottom sealing; Offer some groups of through holes on plate after the front fagging of described lacing wire, flat tube and lacing wire, run through a locking member in every group of through hole.
4. flat bioreactor as claimed in claim 1, is characterized in that, also comprises before lacing wire stiffening plate after stiffening plate and lacing wire; After described lacing wire front end panel, the front stiffening plate of lacing wire, lacing wire rear bearing sheet and lacing wire, stiffening plate is flat board, and described built-in lacing bar is some pipes; Before one end of described pipe and described lacing wire, fagging is fixed, and the other end and described lacing wire rear stay plate are fixed; After the front stiffening plate of described lacing wire and lacing wire, stiffening plate is offered the some group through holes corresponding with described pipe, runs through a locking member in every group of through hole and corresponding pipe.
5. flat bioreactor as claimed in claim 1, is characterized in that, before described lacing wire, fagging comprises the first " L " template and second " L " template, and described lacing wire rear stay plate and described built-in lacing bar are respectively base plate and the intermediate plate of T-shape plate; Offer some groups of through holes on intermediate plate and second " L " template of described first " L " template, T-shape plate, run through a locking member in every group of through hole.
6. flat bioreactor as described in claim 1 ~ 5 any one, is characterized in that, comprises the reactor kerve, is provided with groove on described reactor kerve, and the two side of described groove touch respectively header board and the rear plate of described reactor unit.
7. flat bioreactor as claimed in claim 6, is characterized in that, described reactor kerve is provided with cushion, between the diapire of the base plate of described reactor unit and described groove.
8. flat bioreactor as claimed in claim 7, is characterized in that, described groove is provided with some discharge gates, and each discharge gate is communicated with a reactor unit.
9. flat bioreactor as claimed in claim 8, is characterized in that, described discharge gate comprises drainage conduit and be installed on valve on described drainage conduit, and described drainage conduit is passed the base plate of described groove and described reactor unit.
10. flat bioreactor as claimed in claim 9, is characterized in that, the upper edge of described drainage conduit is lower than the base plate of described reactor unit or concordant with the base plate of described reactor unit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110006738 CN102154093B (en) | 2011-01-13 | 2011-01-13 | Plate type photobioreactor |
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| CN 201110006738 CN102154093B (en) | 2011-01-13 | 2011-01-13 | Plate type photobioreactor |
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| CN102154093B true CN102154093B (en) | 2013-05-15 |
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| TWI505771B (en) * | 2012-02-16 | 2015-11-01 | Far East Bio Tec Co Ltd | Continuous layered three-dimensional microalgae culturing system |
| CN106906116B (en) * | 2015-12-22 | 2020-04-14 | 国投生物科技投资有限公司 | Cleaning device of tube bank type photobioreactor and photobioreactor |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2001269161A (en) * | 2000-03-29 | 2001-10-02 | Research Institute Of Innovative Technology For The Earth | Dome-shaped optically culturing apparatus |
| JP4523187B2 (en) * | 2001-03-15 | 2010-08-11 | 三井造船株式会社 | Photobioreactor |
| CN101405385A (en) * | 2006-02-21 | 2009-04-08 | 亚利桑那州立大学董事会，代表亚利桑那州立大学法人团体利益 | Photobioreactor and uses therefor |
| CN100587054C (en) * | 2006-05-15 | 2010-02-03 | 华东理工大学 | Novel multi-section flat type photo-bioreactor |
| CN201045139Y (en) * | 2006-09-19 | 2008-04-09 | 邹宁 | Industrialized scale optical bioreactor |
| CN201062270Y (en) * | 2007-06-07 | 2008-05-21 | 邹宁 | Industrialized scale economic optical bioreactor |
| US8821431B2 (en) * | 2007-09-07 | 2014-09-02 | Beta O2 Technologies Ltd. | Air gap for supporting cells |
| CN101709264B (en) * | 2009-06-19 | 2012-05-23 | 新奥科技发展有限公司 | Optical bioreactor |
| CN101942388B (en) * | 2009-07-06 | 2014-04-09 | 新奥科技发展有限公司 | Optical bioreactor |
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