CN102151367B

CN102151367B - Micro broad-pulse dual-wavelength optogenetic stimulation device

Info

Publication number: CN102151367B
Application number: CN 201110118258
Authority: CN
Inventors: 张永刚; 顾溢
Original assignee: Shanghai Institute of Microsystem and Information Technology of CAS
Current assignee: Shanghai Institute of Microsystem and Information Technology of CAS
Priority date: 2011-05-09
Filing date: 2011-05-09
Publication date: 2013-04-24
Anticipated expiration: 2031-05-09
Also published as: CN102151367A

Abstract

The invention relates to a micro broad-pulse dual-wavelength optogenetic stimulation device which comprises a small-scale integrated circuit, semiconductor light-emitting devices and switch type driving circuits, wherein the small-scale integrated circuit is respectively connected with two adjustable resistors capable of independently adjusting pulse parameters within large ranges, a changeover switch and an electrical interface; the electrical interface is used for monitoring pulse waveforms and parameters; both ends of the changeover switch are respectively connected with the switch type driving circuits; the two switch type driving circuits are respectively connected with one semiconductor light-emitting device; light pulses of two wavelengths, which are sent out by the driven semiconductor light-emitting devices, are respectively coupled to a light output interface through an optical fiber to be output or are directly output by the optical fiber; and the small-scale integrated circuit is connected with a power supply through a micro voltage-stabilizing module. The invention is applicable to light-emitting diodes (LED) and laser diodes (LD), can be applied to laser diode-pumped solid or optical fiber lasers, has fine universality and generality, and can be implemented in a flexible mode.

Description

A kind of miniature broad pulse scope dual wavelength light sensillary base is because of stimulating apparatus

Technical field

The invention belongs to photoelectron, microelectronics and gene optical technical field, particularly relate to a kind of miniature broad pulse scope dual wavelength light sensillary base because of stimulating apparatus.

Background technology

Introduce electricity irritation at biology and medical domain and produce the history that specific effect or function have centuries, become and used widely a kind of means and technology in these neighborhoods, and entering already clinical medicine, most typical application apparatus is the implantable cardiac pacemaker.Adopt microelectronic circuit to produce specific electric pulse in this type of battery powered device, the microelectrode of input implant into body produces required treatment function.In recent years, the what is called " brain pacemaker " that is used for the treatment of the cerebral nerve disease of the types such as parkinson disease also begins to enter clinical practice, the ultimate principle of its device and function and heart heartstart are quite similar, but the controllability of paired pulses parameter is had higher requirement.Along with the progress of the deep and molecular biotechnology of studying, adopt gene technology in biological cell and organism, to introduce and has bioactive photaesthesia material, utilize its heliosensitivity to produce very big interest and attention that specific biological function has caused people.Nature is through the development in 1 years, a series of bioactive photaesthesia materials that have of having evolved out, and these materials have very strong specificity to the light of specific wavelength in the solar spectrum, but human still insufficient to its understanding, demand carrying out further investigation urgently.In addition, producing the function that the existing function of electricity irritation and exploitation make new advances in the present clinical medicine with photostimulation also is that people dream of.Compare with electricity irritation, can need not to implant in the specific region microelectrode in conjunction with the photostimulation method of gene technology, even might stimulate external, also avoided the aging and fatigue problem of stimulated areas; Utilization also might develop brand-new effect, treatment and control model to the biological photoactive substance of different wave length sensitivity, has broad prospects.In these bases and preclinical applications research, generally adopt the commercial apparatus of general relatively large costliness and device to make up required photostimulation function at present, obviously the requirement of research and development can not be satisfied, easy to use, pulse parameter light stimulation device adjustable, microminiaturized and cheap, low-power consumption on a large scale need to be developed.

Summary of the invention

Technical problem to be solved by this invention provides a kind of miniature broad pulse scope dual wavelength light sensillary base because of stimulating apparatus, can significantly improve the convenience in the light sensation gene studies, reduces to greatest extent and carries out the in this respect threshold of research, promotes the progress of technology.

The technical solution adopted for the present invention to solve the technical problems is: provide a kind of miniature broad pulse scope dual wavelength light sensillary base because of stimulating apparatus, by small scale integration, the formations such as light emitting semiconductor device and switching mode drive circuit, described small scale integration respectively with 2 adjustable resistances of independent regulation pulse parameter on a large scale, change-over switch links to each other with electrical interface, described electrical interface is used for monitoring impulse waveform and parameter, the two ends of described change-over switch are connected with respectively the switching mode drive circuit, described two switching mode drive circuits are connected with respectively a light emitting semiconductor device, the light pulse of two kinds of wavelength that light emitting semiconductor device sends is exported via coupling fiber to light output interface respectively or directly by optical fiber output, described small scale integration links to each other with power supply by the micro voltage stabilizing module.

Described Voltage stabilizing module joins by on and off switch and power supply, and described power supply is lithium battery.

Described lithium battery carries out automatic charging by automatic charging module to it, and described automatic charging module is connected with USB interface.

The present invention adopts cheap general small scale integration, in conjunction with using little, the lightweight light emitting semiconductor device of volume, produce the adjustable two kinds of different wave length pulses on a large scale of pulse frequency and width, be used for biological study and therapeutic treatment is dissimilar and the light sensation genetic recipients of effect stimulate and make it produce corresponding function.The rectangle electric pulse of the Transistor-Transistor Logic level that small scale integration produces, its repetition rate and pulse width can be by adjustable resistance continuous or classification adjustings on a large scale, this electric pulse is sent into the switching mode drive circuit after change-over switch is switched, drive respectively corresponding light emitting semiconductor device, change into the light pulse of the identical required wavelength of pulse parameter, send into simultaneously electrical interface and be used for monitoring impulse waveform and parameter; The light pulse of two kinds of wavelength respectively via coupling fiber to light output interface output or directly by optical fiber output, the intensity of light pulse respectively by adjustable resistance continuously or classification regulate, to satisfy in research and the medical treatment needs to different light pulse parameters and intensity; For the convenient energy of this device that uses is supplied with by the higher chargeable lithium cell of volume energy, in view of the voltage of conventional lithium battery is that 3.7V and voltage are with fluctuating discharge time, and circuit part wishes to power to guarantee that by the burning voltage of 5V pulse parameter is stable, this lithium battery is sent into via on and off switch and is boosted (adopting a joint lithium battery) or voltage-dropping type (adopting the above lithium battery of two joints) micro voltage stabilizing module converts and burning voltage, and the stabilized power source of required voltage is provided for device; Device can carry out automatic charging via automatic charging module by conventional USB interface.

Beneficial effect

The present invention both had been applicable to laser diode LD, also was applicable to LED, also can be applicable to laser diode-pumped solid state laser and optical fiber laser etc., and its wavelength and power can be selected according to the practical application needs; On this device, change a little, the output light wavelength of device can expand to easily two or more or be simplified to a kind of wavelength, the different wave length pulse can be exported respectively or simultaneously output by different optical fiber, also can adopt the optical fiber combiner to be exported by an optical fiber; The different wave length pulse can be produced by same electric pulse generator, also can produce conveniently to be arranged to different pulse parameters with different electric pulse generators; The future developments such as, device implantable, little power consumption can also be further remote-controlled to wavelength and pulse parameter at the basis of this scheme device and contactless charging have good universality and versatility, can realize with the mode of quite flexible.

Description of drawings

Fig. 1 is structural representation of the present invention.

The specific embodiment

Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used for explanation the present invention and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims limited range equally.

As shown in Figure 1, the present invention includes small scale integration, light emitting semiconductor device and switching mode drive circuit, described small scale integration 1 is the adjustable resistance 2 in parallel with 2 respectively, 3, change-over switch 4 links to each other with electrical interface 11, described electrical interface 11 is used for monitoring impulse waveform and parameter, the two ends of described change-over switch 4 are connected with respectively switching mode drive circuit 5,6, described two switching mode drive circuits 5,6 are connected with respectively light emitting semiconductor device 7,8, light emitting semiconductor device 7, the light pulse of 8 two kinds of wavelength that send respectively via coupling fiber to light output interface 9,10 export or are directly exported by optical fiber, and described small scale integration 1 links to each other with power supply by micro voltage stabilizing module 12.

Described Voltage stabilizing module 12 joins by on and off switch 13 and power supply, and described power supply is lithium battery 14.

Described lithium battery 14 carries out automatic charging by automatic charging module 15 to it, and described automatic charging module 15 is connected with usb 16.

Embodiments of the present invention relate to a kind of miniature broad pulse scope dual wavelength light sensillary base because of stimulating apparatus, and implementation step is as follows:

1. adopt a conventional cheap general small-scale 54 or the monostable multi-frequency generator integrated circuit 1 generation pulse frequency of two-way and the width of 74 series to distinguish independent adjustable Transistor-Transistor Logic level rectangle electric pulse on a large scale, its pulse parameter scope is set according to the scope that the light sensation gene stimulates.In view of the requirement of bio-light sensillary base because of the stimulation time constant, the repetition rate General Requirements covers several hertz to up to a hundred hertz scopes, in the device it is arranged on 2-500Hz, and range of accommodation reaches 250 times; The covering of pulse width General Requirements to up to a hundred milliseconds of scopes, is arranged on 0.5-200ms with it in the device below 1 millisecond, and range of accommodation reaches 400 times; Adopt Single Capacitance and ten circle miniature

adjustable resistances

2,3 in the device, the range of accommodation maximum of RC time constant can reach 500 times, can meet the demands, and repetition rate and pulse width are regulated on a large scale continuously; This electric pulse can be monitored its impulse waveform and parameter by electrical interface 11 outputs with oscillograph when pulse parameter is set;

2. adopt two kinds of schemes that different wave length is exported respectively via different fiber in this scheme, the TTL electric pulse is sent into switching mode drive circuit 5,6 after change-over switch 4 is switched, this switching mode drive circuit can directly be made of bipolar transistor, adopt the adjustable series resistance of classification to control its output current, can be controlled at according to concrete device tens of to hundreds of mA; Drive light emitting semiconductor device 7,8 and produce the adjustable two kinds of different wave length pulses on a large scale of pulse frequencies and width.When adopting LED, emission wavelength can be chosen 450-480nm scope blue light commonly used according to the sensitive wave length of light sensation gene, 560-590nm scope gold-tinted and 630-670nm scope HONGGUANG etc., optional wavelength is more, substantially can cover whole limit of visible spectrum, having adopted centre wavelength in this device is that the blue-ray LED of 470nm is as excited light source, the yellow light LED of 590nm is as suppressing light source, silica fibre output via 300 microns core diameters, in view of organism can tolerate photostimulation power plane density every square millimeter of 100mW magnitude, required photostimulation power plane density is below every square millimeter of 10mW during normal operation, and required luminous power is in the mW magnitude when adopting 300 microns core optical fibers; Then optional wavelength is less when adopting the solid state laser of LD or LD pumping, and some wavelength need specific customization, but spectral purity can improve, and luminous power is also larger; Can be directly used in device after luminescent device and the tail optical fiber one end coupling with the required diameter optical fiber of fibre-optical splice;

3. supplied with by a joint rechargeable lithium battary 14 for convenient its energy that uses, this lithium battery is sent into booster type micro voltage stabilizing module via on and off switch 13, be converted to the burning voltage of 5V, for device provides the stabilized power source of required voltage, when adopting the lithium battery of 1Ah, can guarantee stream time more than 12 hours;

4. device adopts 6 pairs of lithium batteries 14 for the device power supply of conventional usb 1 to charge, owing to only used a joint lithium battery, consider that conventional USB interface has the power supply capacity of 5V/100mA, charging module is reduced to one 15 ohmages, available so about 12 hours is the lithium battery full charge of 1Ah, and convenient the employing filled daily mode work night.

By aforementioned implementation step as seen, cheap, conventional, general a small amount of electronic devices and components have only been adopted in this device, adopt the whole device of surface mount mode to realize microminiaturization, can be used for biological study and therapeutic treatment is dissimilar and the light sensation genetic recipients of effect stimulate and make it produce corresponding function.

Claims

1. a miniature broad pulse scope dual wavelength light sensillary base is because of stimulating apparatus, comprise small scale integration, light emitting semiconductor device and switching mode drive circuit, it is characterized in that: described small scale integration (1) respectively with 2 adjustable resistances (2,3), change-over switch (4) links to each other with electrical interface (11), described small scale integration (1) is the monostable multi-frequency generator of two-way of 54 or 74 series, for generation of electric pulse, the repetition rate of this electric pulse and pulse width are by adjustable resistance (2,3) continuous or classification is regulated on a large scale, described electrical interface (11) is used for monitoring impulse waveform and parameter, the two ends of described change-over switch (4) are connected with respectively switching mode drive circuit (5,6), described two switching mode drive circuits (5,6) be connected with respectively light emitting semiconductor device (7,8), light emitting semiconductor device (7,8) send for the light pulse of two kinds of wavelength that stimulate the light sensation genetic recipient respectively via coupling fiber to light output interface (9,10) export or directly exported by optical fiber, described small scale integration (1) links to each other with power supply by micro voltage stabilizing module (12).

2. a kind of miniature broad pulse scope dual wavelength light sensillary base according to claim 1 is characterized in that because of stimulating apparatus: described Voltage stabilizing module (12) joins by on and off switch (13) and power supply, and described power supply is lithium battery (14).

3. a kind of miniature broad pulse scope dual wavelength light sensillary base according to claim 2 is because of stimulating apparatus, it is characterized in that: described lithium battery (14) carries out automatic charging by automatic charging module (15) to it, and described automatic charging module (15) is connected with USB interface (16).