CN102147366A - Super-hydrophobic biochip and optical testing method therefor - Google Patents
Super-hydrophobic biochip and optical testing method therefor Download PDFInfo
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- CN102147366A CN102147366A CN 201010610808 CN201010610808A CN102147366A CN 102147366 A CN102147366 A CN 102147366A CN 201010610808 CN201010610808 CN 201010610808 CN 201010610808 A CN201010610808 A CN 201010610808A CN 102147366 A CN102147366 A CN 102147366A
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Abstract
The invention belongs to the technical field of a biochip, in particular to a super-hydrophobic biochip and an optical testing method therefor. The super-hydrophobic biochip mainly comprises a transparent substrate, a layer of transparent zinc oxide nanorods and a layer of transparent Teflon thin film, wherein the layer of transparent zinc oxide nanorods are tightly combined with the upper surface of the transparent substrate; and the layer of transparent Teflon thin film covers the upper surface of the layer of transparent zinc oxide nanorods; a bioluminescence signal testing system can be established by the super-hydrophobic biochip and comprises a sensitive device, the super-hydrophobic biochip and a liquid drop to be tested which are sequentially arranged from bottom to top; and biological signals of the liquid drop to be tested are spherically converged, penetrate through the super-hydrophobic biochip and are tested by the sensitive device. The super-hydrophobic biochip and the testing method can effectively improve the testing sensitivity of bioluminescence signals.
Description
Technical field
The present invention relates to the biochip technology field, be specifically related to a kind of super-hydrophobic biochip and optical detecting method thereof, specifically, be based on the transparent monoplane opically detecting bio-chip at super-hydrophobic interface and the detection method of faint bioluminescence signal thereof about a kind of.
Background technology
At present traditional optical bio monitoring system often realizes higher detection sensitivity by the sensitization value that improves reactant content or raising sensor devices for the sensitivity that improves optical detection.But some biological respinse reagent price on the one hand is very expensive, is subjected to the restriction of the intrinsic concentration of reactant on the one hand, so be difficult to further improve the content of reactant.This will influence the fluorescence intensity of biological respinse greatly, bring very big difficulty for improving detection sensitivity.
And the sensitization value that improves sensor devices also can improve the detection to faint fluorescence, but because high sensitivity sensor devices cost is higher, and fluorescence is faint more, and ground unrest is strong relatively more, makes the high sensitivity sensor devices also powerless.
Therefore, how to realize the lifting of the sensitivity of Photobiology detection system cheaply, have great significance for biological fluorescent signals.
Summary of the invention
The object of the present invention is to provide a kind of system simple, cheap, can overcome the novel super-hydrophobic biochip and the optical detecting method thereof of the inherent limitation of traditional optical biological monitoring system.
Super-hydrophobic biochip provided by the invention, comprise: a transparent substrates, the zinc oxide nano rod layer of the layer of transparent of combining closely with the upper surface of described transparent substrates, described zinc oxide nano rod is closely vertically arranged each other, forms the needle-like upper surface of a nanostructured; Described needle-like upper surface covers the Teflon film of layer of transparent.
Described transparent substrates thickness is 0.1～0.3mm, and the thickness of described zinc oxide nano rod layer is 100～600nm, and described Teflon film thickness is 50～500nm.
Transparent substrates described in the present invention is made of common cover glass.This cover glass can effectively reduce the lateral scattering and the neighbourhood noise of bioluminescence signal; Described cover glass light transmission is good, can effectively reduce the transmission loss of fluorescence signal through substrate; Described cover glass is cheap, can greatly reduce production cost of the present invention.
Described zinc oxide nano rod layer is grown up by hydro-thermal method and is formed.Earlier at the upper surface of described cover glass by solgel method spin coating one deck zinc paste inculating crystal layer, then by hydro-thermal method, the zinc oxide nano rod that grows vertically and closely arrange in described seed crystal surface.
Described zinc oxide nano rod layer is grown up by hydro-thermal method and is formed.Described zinc paste inculating crystal layer also can be by at the upper surface of the described cover glass method deposit one deck zinc paste inculating crystal layer with plasma sputtering, then by hydro-thermal method, and the zinc oxide nano rod that grows vertically and closely arrange in described seed crystal surface.
Described Teflon film, is annealed in air at upper surface spin coating one deck Teflon of described zinc oxide nano rod then by the method for spin coating, can form described Teflon film.
The optical detecting method of the described super-hydrophobic biochip that the present invention proposes, concrete steps are:
S1, at first set up a bioluminescence signal detection system, comprise and place a sensor devices and described super-hydrophobic biochip from bottom to up successively;
S2, drip bioluminescence drop to be measured at the upper surface of described super-hydrophobic biochip, drop draws globulate in;
Bioluminescence signal in S3, the described bioluminescence drop converges effect through the spherical optics of described bioluminescence drop, focus on downwards, and the transmission of the described super-hydrophobic biochip of process, enter described sensor devices, described sensor devices is converted into electric signal with described bioluminescence signal, according to described electric signal the biomolecule in the described fluorescence drop is analyzed.
The present invention is by forming a super-hydrophobic nanostructured surface, increase the upper surface contact angle of bioluminescence drop and described super-hydrophobic biochip, make described bioluminescence drop draw glomeration in, thereby increase the downward aggregate capabilities of fluorescence signal, thereby make the fluorescence signal intensity that enters described super-hydrophobic biochip below sensor devices increase greatly, by this method, can improve the sensitivity that bioluminescence detects significantly.Described super-hydrophobic biochip can also be produced in batches by microelectronic large-scale production process, greatly reduces the cost of described super-hydrophobic biochip, and has saved expensive biological detection solution.But by the just super-hydrophobic biochip in a kind of transparent monoplane of acquisition of simple and fast of said method, improved the detection sensitivity of bioluminescence signal greatly, reduced the cost of biological detection simultaneously.
Description of drawings
Fig. 1 is a bioluminescence detection system synoptic diagram of the present invention.
Fig. 2 is a manufacturing process synoptic diagram of the present invention.
Fig. 3 is a test result of the present invention.
Number in the figure: 1 is super-hydrophobic biochip; 2 is the bioluminescence drop; 3 is sensor devices; 4 is cover glass; 5 is the zinc oxide nano rod layer; 6 is the Teflon film.
Embodiment
Provide better embodiment of the present invention according to Fig. 1-Fig. 3 below and described in detail, so that the present invention is described better rather than is used for limiting the scope of the invention.
Super-hydrophobic biochip provided by the invention is made of a super-hydrophobic nanostructured surface and a common cover glass substrate.Above-mentioned nanostructured surface is made of the Teflon film that the needle shape zinc oxide nano stick of closely arranging and this nanorod surfaces cover, and has super-hydrophobic and transparent effect.Above-mentioned zinc oxide nano rod forms by hydrothermal growth.Above-mentioned Teflon film forms by spin-coating method and annealing.The substrate of above-mentioned super-hydrophobic biochip is a common cover glass, and light transmission is good and cheap.Pass through said structure, can make the bioluminescence drop form the solid-liquid interface contact angle of spending greater than 150 at above-mentioned super-hydrophobic nanostructured surface, make drop draw glomeration in, thereby increased the downward aggregate capabilities of drop to fluorescence, make the fluorescence signal that enters chip below sensor devices strengthen greatly, can improve the sensitivity that bioluminescence detects significantly.Above-mentioned super-hydrophobic biochip can also be produced in batches by microelectronic large-scale production process, greatly reduces the cost of above-mentioned super-hydrophobic biochip, and has saved expensive biological detection solution.But by the just super-hydrophobic biochip in a kind of transparent monoplane of acquisition of simple and fast of said method, improved the detection sensitivity of bioluminescence signal greatly, reduced the cost of biological detection simultaneously.
As shown in Figure 1, bioluminescence detection system of the present invention comprises sensor devices 3, super-hydrophobic biochip 1 and the bioluminescence drop of placing successively from bottom to up 2.Bioluminescence signal in the described bioluminescence drop 2 converges effect through the spherical optics of described bioluminescence drop 2, focus on downwards, and the transmission of the described super-hydrophobic biochip 1 of process, enter described sensor devices 3, described sensor devices 3 is converted into electric signal with described bioluminescence signal, can analyze the biomolecule in the described fluorescence drop 2 according to described electric signal.
As shown in Figure 2, the preparation process of described super-hydrophobic biochip 1 of the present invention was made up of two steps, and method is simple, and is with low cost.At first on a transparent cover glass 4, pass through solgel method spin coating one deck zinc paste inculating crystal layer, 6000 rpms of rotating speeds, 100 degree annealing earlier are 5 minutes then, and 300 degree were annealed 10 minutes again.Then by hydro-thermal method, at described seed crystal surface growing zinc oxide nanorod layer 5.Detailed process is for having the zinc paste inculating crystal layer with spin coating and moving back fiery described cover glass 4 and put into 75 degrees centigrade Zn (CH
3COO)
2-2H
20 and the MEA mixed solution in, leave standstill the zinc oxide nano rod that can grow vertically and closely arrange in 5 hours, 400 degrees centigrade of AN 15 minutes, can form stable zinc oxide nano rod layer 5 at last.
Second step is at upper surface spin coating one deck Teflon of described zinc oxide nano rod layer 5, rotating speed is 6000 rpms, in 115 degrees centigrade of air, annealed 10 minutes then, then in 245 degree air, annealed 5 minutes, in 330 degree air, annealed 15 minutes at last, can form stable Teflon film 6.A stable super-hydrophobic optical detection chip 1 just prepares to be finished.
Figure 3 shows that and utilize described super-hydrophobic biochip 1 and described sensor devices 3 to detect the sheep anti-mouse igg-HRP biomolecule solution for preparing, and under the effect of zymolyte, can send light blue fluorescence.By changing the solid-liquid contact angle of described solution droplets and described super-hydrophobic biochip 1, it is big more that we can obtain contact angle, and the bioluminescence signal that detects is strong more.So by described super-hydrophobic biochip of the present invention, can significantly strengthen the sensitivity that bioluminescence detects, and method is simple, with low cost.
Claims (7)
1. super-hydrophobic biochip is characterized in that comprising:
A transparent substrates;
The zinc oxide nano rod layer of the layer of transparent of combining closely with the upper surface of described transparent substrates, described zinc oxide nano rod is closely vertically arranged each other, forms the needle-like upper surface of a nanostructured;
Cover the Teflon film of the layer of transparent of described needle-like upper surface.
2. according to the described super-hydrophobic biochip of claim 1, it is characterized in that described transparent substrates is a cover glass.
3. according to the described super-hydrophobic biochip of claim 2, it is characterized in that, described zinc oxide nano rod layer is grown up by hydro-thermal method and formed: the upper surface at described cover glass passes through solgel method spin coating one deck zinc paste inculating crystal layer earlier, then by hydro-thermal method, the zinc oxide nano rod that grows vertically and closely arrange in described seed crystal surface.
4. according to the described super-hydrophobic biochip of claim 2, it is characterized in that, described zinc oxide nano rod layer is grown up by following method and is formed: method deposit one deck zinc paste inculating crystal layer of using plasma sputtering at the upper surface of described cover glass, then by hydro-thermal method, the zinc oxide nano rod that grows vertically and closely arrange in described seed crystal surface;
5. according to claim 3 or 4 described super-hydrophobic biochips, it is characterized in that, described Teflon film prepares by spin-coating method: at described zinc oxide nano rod layer upper surface spin coating one deck Teflon, anneal in air then, promptly form described Teflon film.
6. according to the described super-hydrophobic biochip of one of claim 1-4, it is characterized in that described transparent substrates thickness is 0.1～0.3mm, the thickness of described zinc oxide nano rod layer is 100～600nm, and described Teflon film thickness is 50～500nm.
7. the optical detecting method of a super-hydrophobic biochip as claimed in claim 1 is characterized in that concrete steps are:
S1, at first set up a bioluminescence signal detection system, comprise and place a sensor devices and described super-hydrophobic biochip from bottom to up successively;
S2, drip bioluminescence drop to be measured at the upper surface of described super-hydrophobic biochip, drop draws globulate in;
Bioluminescence signal in S3, the described bioluminescence drop converges effect through the spherical optics of described bioluminescence drop, focus on downwards, and the transmission of the described super-hydrophobic biochip of process, enter described sensor devices, described sensor devices is converted into electric signal with described bioluminescence signal, according to described electric signal the biomolecule in the described fluorescence drop is analyzed.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107974679A (en) * | 2017-11-22 | 2018-05-01 | 西北大学 | A kind of green can anti-corrosion tube wall film preparation method |
Citations (5)
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|---|---|---|---|---|
| CN1379128A (en) * | 2001-04-06 | 2002-11-13 | 中国科学院化学研究所 | Ultra-amphosphobic film and its preparing process |
| CN1940561A (en) * | 2005-09-27 | 2007-04-04 | 横河电机株式会社 | Biochip reading apparatus and biochip reading method |
| US20070210349A1 (en) * | 2002-06-06 | 2007-09-13 | Yicheng Lu | Multifunctional biosensor based on ZnO nanostructures |
| CN101285168A (en) * | 2008-01-11 | 2008-10-15 | 北京工业大学 | Preparation method of porous conducting nano copper film material with ultra- hydrophobicity |
| CN101613872A (en) * | 2009-07-23 | 2009-12-30 | 东南大学 | Method for preparing super-hydrophobic surface with electro-wetting property |
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2010
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1379128A (en) * | 2001-04-06 | 2002-11-13 | 中国科学院化学研究所 | Ultra-amphosphobic film and its preparing process |
| US20070210349A1 (en) * | 2002-06-06 | 2007-09-13 | Yicheng Lu | Multifunctional biosensor based on ZnO nanostructures |
| CN1940561A (en) * | 2005-09-27 | 2007-04-04 | 横河电机株式会社 | Biochip reading apparatus and biochip reading method |
| CN101285168A (en) * | 2008-01-11 | 2008-10-15 | 北京工业大学 | Preparation method of porous conducting nano copper film material with ultra- hydrophobicity |
| CN101613872A (en) * | 2009-07-23 | 2009-12-30 | 东南大学 | Method for preparing super-hydrophobic surface with electro-wetting property |
Non-Patent Citations (2)
| Title |
|---|
| 《Applied Surface Science》 20101001 N.L. Tarwal ET AL Superhydrophobic and transparent ZnO thin films synthesized by spray pyrolysis technique 全文 1-7 第256卷, 第24期 * |
| 《物理学报》 20090331 公茂刚等 两步法制备超疏水性ZnO纳米棒薄膜 1885-1888 1-7 第58卷, 第3期 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107974679A (en) * | 2017-11-22 | 2018-05-01 | 西北大学 | A kind of green can anti-corrosion tube wall film preparation method |
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Application publication date: 20110810 |