CN102146448A - DNA finger prints of 30 main cultivars of upland cotton - Google Patents
DNA finger prints of 30 main cultivars of upland cotton Download PDFInfo
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- CN102146448A CN102146448A CN 201110005936 CN201110005936A CN102146448A CN 102146448 A CN102146448 A CN 102146448A CN 201110005936 CN201110005936 CN 201110005936 CN 201110005936 A CN201110005936 A CN 201110005936A CN 102146448 A CN102146448 A CN 102146448A
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Abstract
The invention belongs to the field of agricultural biotechnology. Currently, the condition of low purity of crop seeds is common in China, and all levels of crop variety approval organizations and seed production departments attach increasing importance to the identification of the purity of variety. The research of the invention constructs the DNA finger prints of 30 main cultivars of upland cotton which are planted at one time or at present in different ecological cotton regions in China by an SSR (Simple Sequence Repeats) molecular marker technique. Indicated by electrophoresis results of 20 pairs of SSR core primers with good repeatability and high polymorphism, 4 varieties of the 30 varieties have the respective specific primers and can be differentiated from other varieties; and the rest 26 varieties can be differentiated from other varieties by at least 2 pairs of primer combinations. To identify every variety more conveniently and accurately, digital fingerprint codes of 20 pairs of core primers of 30 varieties are constructed. The invention provides a theoretical basis for authenticity identification, breeding utilization and interest protection of new varieties of the 30 main cultivars of upland cotton, and lays an important foundation for constructing a standard fingerprint library of the bred cotton varieties in China.
Description
Technical field
The invention belongs to agricultural biological technical field, especially utilize molecular marking technique that kind is carried out dna fingerprinting and make up, it is easy, quick, accurate to have realized that kind is differentiated.
Background technology
The inferior situation ubiquity of current China crop seeds purity, varieties of crops at different levels city decides mechanism and seed production department identifies pay attention to day by day to variety, therefore, employing is efficient, the accurate detection method carries out cultivar identification and purity detecting is very necessary.The various molecular marking techniques based on the DNA variation that last century, the eighties was risen have driven the fast development of dna fingerprinting technology.Difference between the direct inverted sequence genomic dna of the polymorphism that this electrophoretogram disclosed, not affected by environment, quantity is many, accuracy is high, individual specificity and environmental stability with height, be called as " dna fingerprinting ", utilizing molecular marking technique constructed dna finger printing is the strong instrument of differential variety or strain.At present, utilize various molecular marking techniques, many important crops such as tomato, rape, paddy rice, wheat, corn, soybean etc. have all carried out the research of cultivar identification and purity detecting, and wherein, the SSR fingerprints database of main breeds such as tomato, paddy rice and wheat has made up to be finished.More existing reports of fingerprint map construction research of cotton.Yet some researchs are owing to be in the commitment of molecule marker development, and its examination material that supplies that is used for fingerprint map construction mainly is confined to less target material, and 30 cotton main breeds that this paper relates to are not seen relevant report.Since a certain primer amplification to the allelotrope number can increase with the increase of building library material, utilize limited examination material to carry out the reduction that the fingerprinting meeting causes the polymorphic quantity of information of primer, thereby influence the specificity of target material collection of illustrative plates.If increased other background materials under study for action, the special primer of some target materials is not unique just probably.
This research and utilization SSR molecular marking technique; to in China different ecological cotton region once or planting 30 cotton main breeds; carry out the dna fingerprinting analysis; the hereditary basis of confession examination material is abundant and quantity is more; utilize the special primer or the combination of primers of each material; and the digital finger-print code of 20 pairs of core primers, can be easy, the differential variety purity and the true and false quickly and accurately, effectively protect the intellecture property of kind and the rights and interests of breeding man.
Summary of the invention
With China different ecological cotton region once or 30 upland cotton main breeds that are mainly derived from Mount Tai word cotton, this word cotton, good fortune word cotton, Uganda cotton of planting be material (table 1), screen 20 pairs of core primers of good reproducibility, rich polymorphism the 2503 pairs of SSR primers (mark) on being positioned at 26 karyomit(e)s of cotton.The electrophoresis result of 20 pairs of core primers shows, there are 4 kinds to have separately special primer in 30 kinds, itself and other variety plot can be separated, wherein, middle cotton 64 special primer have 2 to be respectively NAU4903 and DPL0209, the special primer of the Liao Dynasty cotton 19 has 2 to be respectively NAU2251 and NAU2092, and the special primer that nasal mucus is cotton No. 4 is NAU4903, and prosperous autumn No. 1 special primer is NAU1043 (Fig. 1, Fig. 3); Other 26 kinds can utilize at least 2 pairs of combination of primers and other variety plots to separate, as utilize primer NAU2092/NAU1043 Ji cotton 958 can be separated (Fig. 2, Fig. 3) with other variety plots; For more convenient, differentiate each kind exactly, improve it and produce the separating capacity that goes up other kinds, made up the tens digit finger-print code (table 2) of 20 pairs of core primers of 30 kinds.
The title of table 130 main breed, pedigree source, breeding time type and authorization time
The tens digit finger-print code of 20 pairs of core primers of table 230 main breed
The present invention innovates part
The dna fingerprinting of 30 upland cotton main breeds did not appear in the newspapers in the past, and the digital finger-print of 20 pairs of SSR core primers of the particularly combination of primers of the special primer of 4 kinds, 26 kinds, and all kinds does not appear in the newspapers.The present invention at first screens 20 pairs of SSR core primers that are used for the cotton fingerprint map construction; by electrophoretic analysis; the special primer or the combination of primers finger printing of each kind are combined with digital finger-print information; improve the distinguishing ability of each kind and other kinds greatly, effectively protected the intellecture property of kind and the rights and interests of breeding man.
Description of drawings
Fig. 1: 1-30 is respectively respective material in the table 1; The arrow indication is the special primer NAU2092 finger printing of No. 7 kind the Liao Dynasty cotton 19.
Fig. 2: 1-30 is respectively respective material in the table 1; The arrow indication is the combination of primers finger printing of No. 20 kind Ji cottons 958; Last figure is primer NAU2092, and figure below is primer NAU1043.
Fig. 3: 1-2: middle cotton 64 special primer NAU4903, DPL0209; 3-4: special primer NAU2251, the NAU2092 of the Liao Dynasty cotton 19; 5: the special primer NAU4903 that nasal mucus is cotton No. 4; 6: No. 1 special primer NAU1043 of prosperous autumn; 7-8: middle cotton 12 combination of primers NAU1255/NAU2251; 9-10: middle cotton 41 combination of primers NAU1043/NAU5104; 11-12: middle cotton 45 combination of primers.
Claims (5)
1.30 the dna fingerprinting of individual cotton main breed is characterized in that: 30 cotton main breeds comprise: middle cotton institute 12, middle cotton institute 41, middle cotton institute 45, middle cotton institute 50, middle cotton institute 58, middle cotton institute 64, the Liao Dynasty are cotton 19, the Shandong cotton grinds 19, the Shandong cotton grinds 21, the Shandong cotton grinds 22, the Shandong cotton grinds 28, the Shandong cotton grinds 29, Shanxi cotton 29, Shanxi cotton 36, Shanxi cotton 45, cotton No. 3 of nasal mucus, cotton No. 4 of nasal mucus, Henan cotton 15, Shan 2365, Ji cotton 958, Handan cotton 802, Handan 885, Xuzhou 142, Xuzhou 514, prosperous autumn No. 1, hundred cotton No. 1, SGK958, glad cotton No. 3 of state, cotton No. 4 of section, in plant cotton No. 2; Dna fingerprinting refers to the dna fingerprinting based on the SSR molecule marker.
2. content according to claim 1 is characterized in that: refer to the special primer finger printing and the combination of primers finger printing of 30 kinds and the digital finger-print code of 20 pairs of core primers based on the dna fingerprinting of SSR molecule marker.
3. content according to claim 2 is characterized in that: special primer finger printing or combination of primers finger printing must be the special primer of 4 kinds obtaining of this research and the combination of primers of 26 kinds.Specific as follows: middle cotton 64 special primer be NAU4903 and DPL0209; The special primer of the Liao Dynasty cotton 19 is NAU2251 and NAU2092; The special primer that nasal mucus is cotton No. 4 is NAU4903; Prosperous autumn No. 1 special primer is NAU1043; Middle cotton 12 combination of primers be NAU1255/NAU2251; Middle cotton 41 combination of primers be NAU1043/NAU5104; Middle cotton 45 combination of primers be NAU0905/NAU2627; Middle cotton 50 combination of primers be NAU1085/NAU3110; Middle cotton 58 combination of primers be NAU1043/NAU1085; It is NAU1255/NAU5262 that the Shandong cotton is ground 19 combination of primers; It is NAU0905/NAU2092 that the Shandong cotton is ground 21 combination of primers; It is NAU1043/NAU2121 that the Shandong cotton is ground 22 combination of primers; It is NAU3110/NAU4024/NAU5104 that the Shandong cotton is ground 28 combination of primers; It is NAU1028/NAU5104 that the Shandong cotton is ground 29 combination of primers; The combination of primers of Shanxi cotton 29 is NAU0905/NAU1085; The combination of primers of Shanxi cotton 36 is NAU2251/NAU3839; The combination of primers of Shanxi cotton 45 is NAU2251/NAU5262/NAU2092; The combination of primers that nasal mucus is cotton No. 3 is DPL0209/NAU1085; The combination of primers of Henan cotton 15 is NAU1043/NAU2627; The combination of primers in Shan 2365 is NAU2869/NAU5104; The combination of primers of Ji cotton 958 is NAU2092/NAU1043; The combination of primers of Handan cotton 802 is DPL0209/NAU1028; The combination of primers in Handan 885 is NAU2092/NAU4024; The combination of primers in Xuzhou 142 is NAU2092/NAU4024/NAU1043; The combination of primers in Xuzhou 514 is NAU1085/NAU3703; The combination of primers that hundred cottons are No. 1 is NAU1028/NAU5104; The combination of primers of SGK958 is NAU1085/NAU2627; Glad cotton No. 3 combination of primers of state is DPL0209/NAU1255; The combination of primers that section is cotton No. 4 is NAU2092/NAU2627; In to plant cotton No. 2 combination of primers be NAU3110/NAU3703.
4. content according to claim 2, it is characterized in that: the digital finger-print code of 20 pairs of core primers, must be 20 pairs of core primers that this research obtains, be respectively NAU1028, NAU3703, NAU5104, NAU0905, NAU2627, NAU1255, NAU2092,, NAU3337, NAU4024, NAU5262, NAU3110, NAU2251, NAU1043, DPL0209, NAU1085, NAU3254, NAU2869, NAU3839, NAU4903 and NAU2121; The digital finger-print code is the decimal system or the corresponding binary digit finger-print code of these primers.
5. content according to claim 4 is characterized in that: 20 pairs of core primers ten's digit word codes of 30 kinds see the following form respectively, and its corresponding binary digit finger-print code is corresponding one by one with its information, and the present invention does not do and gives unnecessary details.
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| CN 201110005936 CN102146448A (en) | 2011-01-13 | 2011-01-13 | DNA finger prints of 30 main cultivars of upland cotton |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103049725A (en) * | 2012-11-21 | 2013-04-17 | 安徽省农业科学院水稻研究所 | Crop variety identity card management method |
| CN103060430A (en) * | 2012-03-22 | 2013-04-24 | 艾先涛 | Primers for identifying cotton seeds and kit thereof |
| CN105624289A (en) * | 2016-01-07 | 2016-06-01 | 中国农业科学院棉花研究所 | Primer group, application of primer group, and method for performing cotton germplasm resource genetic diversity analysis by using the primer group |
| CN108570513A (en) * | 2018-04-24 | 2018-09-25 | 中国农业科学院棉花研究所 | SSR primers for building Upland Cotton finger-print and its application |
| CN108570514A (en) * | 2018-04-24 | 2018-09-25 | 中国农业科学院棉花研究所 | SSR primers for building 50 finger-print of nakamise and its application |
| CN113637793A (en) * | 2021-09-15 | 2021-11-12 | 石河子农业科学研究院 | DNA fingerprint construction of new cotton stone K28 based on SSR marker |
-
2011
- 2011-01-13 CN CN 201110005936 patent/CN102146448A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103060430A (en) * | 2012-03-22 | 2013-04-24 | 艾先涛 | Primers for identifying cotton seeds and kit thereof |
| CN103060430B (en) * | 2012-03-22 | 2014-06-18 | 艾先涛 | Primers for identifying cotton seeds and kit thereof |
| CN103049725A (en) * | 2012-11-21 | 2013-04-17 | 安徽省农业科学院水稻研究所 | Crop variety identity card management method |
| CN103049725B (en) * | 2012-11-21 | 2015-05-06 | 安徽省农业科学院水稻研究所 | Crop variety identity card management method |
| CN105624289A (en) * | 2016-01-07 | 2016-06-01 | 中国农业科学院棉花研究所 | Primer group, application of primer group, and method for performing cotton germplasm resource genetic diversity analysis by using the primer group |
| CN105624289B (en) * | 2016-01-07 | 2019-05-21 | 中国农业科学院棉花研究所 | Primer sets and its application utilize the method for primer sets progress germplasm resource for cotton analysis of genetic diversity |
| CN108570513A (en) * | 2018-04-24 | 2018-09-25 | 中国农业科学院棉花研究所 | SSR primers for building Upland Cotton finger-print and its application |
| CN108570514A (en) * | 2018-04-24 | 2018-09-25 | 中国农业科学院棉花研究所 | SSR primers for building 50 finger-print of nakamise and its application |
| CN108570514B (en) * | 2018-04-24 | 2021-10-29 | 中国农业科学院棉花研究所 | SSR primer for constructing 50 fingerprint of Zhongmian institute and application thereof |
| CN108570513B (en) * | 2018-04-24 | 2021-10-29 | 中国农业科学院棉花研究所 | SSR primer for constructing upland cotton variety fingerprint spectrum and application thereof |
| CN113637793A (en) * | 2021-09-15 | 2021-11-12 | 石河子农业科学研究院 | DNA fingerprint construction of new cotton stone K28 based on SSR marker |
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Application publication date: 20110810 |