CN102144542A - Method for preventing tissue-cultured thrips from being polluted - Google Patents
Method for preventing tissue-cultured thrips from being polluted Download PDFInfo
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- CN102144542A CN102144542A CN 201010620976 CN201010620976A CN102144542A CN 102144542 A CN102144542 A CN 102144542A CN 201010620976 CN201010620976 CN 201010620976 CN 201010620976 A CN201010620976 A CN 201010620976A CN 102144542 A CN102144542 A CN 102144542A
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Abstract
The invention discloses a method for preventing tissue-cultured thrips from being polluted. The method comprises the following steps of: 1) covering a cover on a culture vessel after the conventional tissue culture and inoculation, or plugging a stopper into the culture vessel; 2) spreading or spraying a thrips killing agent at the contact position of the lower edge of the cover and the culture vessel or at the contact position of the mouth of the culture vessel and the stopper; and 3) sealing a sealing film at the contact position of the lower edge of the cover and the culture vessel or at the contact position of the mouth of the culture vessel and the stopper. The invention has the advantages that: by the method, the pollution of plant tissue-cultured thrips and the pollution of fungi, bacteria and the like caused by the pollution of the thrips can be effectively prevented, the production efficiency can be greatly improved, and the production cost can be reduced; and the method is favorable for large-scale industrial production.
Description
Technical field
The present invention relates to plant tissue culture fast breeding technique field, especially a kind of method that prevents to organize the pollution of training thrips.
Background technology
Plant tissue culture technique is to use one of the most successful biotechnology in recent decades in the world.Cultivation, aspects such as quickening breeding process, good variety selection and the preservation of germ plasm resource low temperature at Clonal Rapid Propagation, detoxic seedling have all obtained tangible economic benefit, social benefit and ecological benefits, and its effect on research and production are used is also more and more important.But in the industrialization production process of tissue-culturing rapid propagation, topmost problem is exactly the frequent generation that the group training is polluted.The group training is polluted and is not only had a strong impact on the quality and the output of tissue cultivating seedling, but also increases substantially production cost.It is reported that pollute 5 percentage points of every increases, cost will increase progressively more than 10%; Pollution rate is high more, and increasing costs is big more, and when pollution rate reaches 30%, cost will increase to 106.5% (Zhu Dewei, 2001).Therefore, have only to reduce as far as possible and pollute, reduce the waste of human and material resources and financial resources, just might obtain favorable economic benefit.
Plant tissue culture pollution source kind is a lot, studies more have fungi, bacterium and endogenetic fungus (He Yunfang, 1997; Lie group, 2001; Dong Yingping, 2001; Letter is emerging, and 2003; Zhou Junhui, 2003; Wang Yiting, 2005), but in investigation of pollution sources research, thrips also is the plant tissue culture important pollution sources.Report was once arranged abroad, and one group of training enterprise endangers because of being subjected to thrips, and tissue cultivating seedling is all contaminated, and loss is very heavy.In general, external group is trained the history of the existing recent two decades of industry development, and facilities and equipment, environmental condition and peopleware are better than China, and standard is compared in the management that group training industrialization is produced, and using more rational anti-pollution medicament, the pollution rate of group training can be controlled in 5%.But China is owing to group training industry just starts to walk, particularly the Jiangsu and Zhejiang Provinces local climate is warm wet, bacteria containing amount height in the air, the group training is not mostly isolated around the chamber, small worms such as ant, thrips, spider are easy to creep in the inlet chamber culture vessel, cause a large amount of pollutions, and the pollution rate of tissue cultivating seedling often substantially exceeds 5%, that have even surpass 50%, had a strong impact on economic benefit of enterprises.
Summary of the invention
Purpose of the present invention is just in order to overcome the deficiency of above-mentioned technology, and a kind of method that the training thrips pollutes that prevents to organize is provided.
The present invention solves the technical scheme that its technical problem adopts: this preventing organized the method that the training thrips pollutes, and carries out as follows:
(1), culture vessel is built lid in routine group training inoculation back, or the good stopper of culture vessel plug;
(2), lid following smear with the stopper contact position or spray with culture vessel contact position or culture vessel mouth shine thrips agent extremely;
(3), seal film following the sealing up of lid with culture vessel contact position or culture vessel mouth and stopper contact position.
Described culture vessel comprises transparent plastic culture vessel, glass blake bottle, triangular flask or test tube.
Describedly kill the thrips agent, comprise 2000 times of 1000 times of suspending agents of 2.5% dish happiness, 1000 times of 10% imidacloprid wettable powders or 20% good year winter missible oil.
The described film that seals comprises group training special-purpose sealing film, Palafilm film, preservative film.
Described group of training thrips pollutes, and comprises that palm thrips (Thrips palmi Karny), golden thistle horse (Trips flavusSchrank) and thrips pollute pollutions such as the fungi that causes, bacterium.
The effect that the present invention is useful: by method of the present invention, can effectively prevent the plant tissue culture thrips to pollute and thrips pollutes pollutions such as the fungi that causes, bacterium, enhance productivity greatly, reduce production costs, help the batch production large-scale production.
Embodiment
The invention will be further described below in conjunction with embodiment:
Embodiment 1:
A kind of preventing organized the method that the training thrips pollutes, and carries out as follows:
1), culture vessel is built lid in routine group training inoculation back, or the good stopper of culture vessel plug; 2), lid following smear with the stopper contact position or spray with culture vessel contact position or culture vessel mouth shine thrips agent extremely; 3), seal film following the sealing up of lid with culture vessel contact position or culture vessel mouth and stopper contact position.
Described culture vessel comprises transparent plastic culture vessel, glass blake bottle, triangular flask, test tube.
Describedly kill the thrips agent, comprise that 1000 times of 2.5% dish happiness suspending agents 1000 times, a time clean (10% imidacloprid wettable powder) or 20% get well 2000 times of year winter missible oil.
The described film that seals comprises group training special-purpose sealing film, Palafilm film, preservative film.
Described group of training thrips pollutes, and comprises that palm thrips (Thrips palmi Karny), golden thistle horse (Trips flavusSchrank) and thrips pollute pollutions such as the fungi that causes, bacterium.
Embodiment 2:
In this example, 1), builds lid at routine group training inoculation back culture vessel; 2), kill the thrips agent following solarization of lid with the spray of culture vessel contact position; 3), seal film following the sealing up of lid with the culture vessel contact position.
Described culture vessel comprises transparent plastic culture vessel, glass blake bottle.
Described thrips agent extremely is 1000 times of 2.5% dish happiness suspending agents.
The described film that seals is a preservative film.
Described group of training killed the thrips agent and polluted, and comprises that palm thrips (Thrips palmi Karny), golden thistle horse (Trips flavusSchrank) and thrips pollute pollutions such as the fungi that causes, bacterium.
Embodiment 3:
In this example, 1), at the good stopper of routine group training inoculation back culture vessel plug; 2), be coated with in culture vessel mouth and stopper contact position and obliterate the thrips agent; 3), seal up in culture vessel mouth and stopper contact position and seal film.
Described culture vessel comprises transparent triangular flask, test tube.
Described thrips agent extremely is clean (10% imidacloprid wettable powder) 1000 times.
The described film that seals is group training special-purpose sealing film, Palafilm film.
Described group of training thrips pollutes, and comprises that palm thrips (Thrips palmi Karny), golden thistle horse (Trips flavusSchrank) and thrips pollute pollutions such as the fungi that causes, bacterium.
Embodiment 4:
In this example, 1), culture vessel is built lid in routine group training inoculation back, or the good stopper of culture vessel plug; 2), obliterate the thrips agent following being coated with of lid with culture vessel contact position or culture vessel mouth and stopper contact position; 3), seal film following the sealing up of lid with culture vessel contact position or culture vessel mouth and stopper contact position.
Described culture vessel comprises transparent plastic culture vessel, glass blake bottle, triangular flask, test tube etc.
Described thrips agent extremely is 2000 times of 20% good year winter missible oil.
The described film that seals comprises group training special-purpose sealing film, Palafilm film, preservative film etc.
The described method that prevents to organize the pollution of training thrips, it is characterized in that described group of training thrips pollutes, comprise that palm thrips (Thrips palmi Karny), golden thistle horse (Trips flavus Schrank) and thrips pollute pollutions such as the fungi that causes, bacterium.
In addition to the implementation, the present invention can also have other embodiments.All employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop on the protection domain of requirement of the present invention.
Claims (4)
1. one kind prevents to organize the method that the training thrips pollutes, and it is characterized in that: carry out as follows:
(1), culture vessel is built lid in routine group training inoculation back, or the good stopper of culture vessel plug;
(2), lid following smear with the stopper contact position or spray with culture vessel contact position or culture vessel mouth shine thrips agent extremely;
(3), seal film following the sealing up of lid with culture vessel contact position or culture vessel mouth and stopper contact position.
2. according to claim 1 preventing organized the method that the training thrips pollutes, and it is characterized in that: described culture vessel comprises transparent plastic culture vessel, glass blake bottle, triangular flask or test tube.
3. according to claim 1ly prevent to organize the method that the training thrips pollutes, it is characterized in that: describedly kill the thrips agent, comprise 2000 times of 1000 times of suspending agents of 2.5% dish happiness, 1000 times of 10% imidacloprid wettable powders or 20% good year winter missible oil.
4. according to claim 1 preventing organized the method that the training thrips pollutes, and it is characterized in that: the described film that seals comprises group training special-purpose sealing film, Palafilm film, preservative film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010620976 CN102144542A (en) | 2010-12-24 | 2010-12-24 | Method for preventing tissue-cultured thrips from being polluted |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010620976 CN102144542A (en) | 2010-12-24 | 2010-12-24 | Method for preventing tissue-cultured thrips from being polluted |
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| Publication Number | Publication Date |
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| CN102144542A true CN102144542A (en) | 2011-08-10 |
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| CN 201010620976 Pending CN102144542A (en) | 2010-12-24 | 2010-12-24 | Method for preventing tissue-cultured thrips from being polluted |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102577960A (en) * | 2012-02-27 | 2012-07-18 | 江阴沃土农业生物科技开发有限公司 | Tissue culture method of potatoes |
| CN102771390A (en) * | 2012-07-09 | 2012-11-14 | 江西省喜果绿化有限公司 | Method for preventing and treating thrips in sealed tissue culture bottle seedlings |
| CN103563647A (en) * | 2012-08-07 | 2014-02-12 | 北京中怡远大机电设备有限公司 | Method for processing fungal contamination in cordyceps militaris culture process |
| CN105647818A (en) * | 2016-04-12 | 2016-06-08 | 广西大学 | Plate cultivation method for preventing pollution |
-
2010
- 2010-12-24 CN CN 201010620976 patent/CN102144542A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| 《中国优秀博硕士学位论文全文数据库（硕士） 基础科学辑》 20021231 马翠萍 "植物组培污染防治的研究" 全文 1-4 , 第2期 * |
| 《北方园艺》 20070815 屈云慧等 "花卉组织培养中污染的发生与防治" 第188-189页 1-4 , 第8期 * |
| 《北方园艺》 20101015 肖桔清等 "西花蓟马对安祖花组培苗及其移栽苗危害的初步研究" 第160-162页 1-4 , 第19期 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102577960A (en) * | 2012-02-27 | 2012-07-18 | 江阴沃土农业生物科技开发有限公司 | Tissue culture method of potatoes |
| CN102771390A (en) * | 2012-07-09 | 2012-11-14 | 江西省喜果绿化有限公司 | Method for preventing and treating thrips in sealed tissue culture bottle seedlings |
| CN103563647A (en) * | 2012-08-07 | 2014-02-12 | 北京中怡远大机电设备有限公司 | Method for processing fungal contamination in cordyceps militaris culture process |
| CN103563647B (en) * | 2012-08-07 | 2016-01-20 | 北京中怡远大机电设备有限公司 | The processing method of fungal contamination in a kind of cordyceps militaris plantation process |
| CN105647818A (en) * | 2016-04-12 | 2016-06-08 | 广西大学 | Plate cultivation method for preventing pollution |
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Application publication date: 20110810 |