CN102140130B - Antibacterial peptide and use thereof - Google Patents
Antibacterial peptide and use thereof Download PDFInfo
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- CN102140130B CN102140130B CN 201010552417 CN201010552417A CN102140130B CN 102140130 B CN102140130 B CN 102140130B CN 201010552417 CN201010552417 CN 201010552417 CN 201010552417 A CN201010552417 A CN 201010552417A CN 102140130 B CN102140130 B CN 102140130B
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- antibacterial peptide
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- fish
- antibacterial
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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Abstract
The invention relates to the field of molecular microbiology, in particular to antibacterial peptide and use thereof. The antibacterial peptide T1-17 is represented by an amino acid sequence SEQ ID No.1 in a sequence table. The antibacterial peptide T1-17 has a bacterial inhibition function. The antibacterial peptide has a high-efficiency bacterial inhibition function and can effectively inhibit various kinds of fish pathogenic bacteria. Thus, the antibacterial peptide is expected to be used for preventing and controlling fish diseases.
Description
Technical field
The present invention relates to field of molecular microbiology, specifically a kind of antibacterial peptide and application thereof.
Background technology
Along with improving constantly of fishery intensive culture degree, water area ecological environment has suffered havoc, causes aquatic animal disease to be on the increase, and the disease problem has become the bottleneck of culture fishery sustainable development.Traditional antibiotic therapy not only causes the generation of bacterial drug resistance, and has caused drug residue, has a strong impact on the quality of fishery products, even threatens human health.Therefore, seeking new disease-resistance substance replaces microbiotic just to seem very urgent.Antibacterial peptide is that a class extensively is present in the micromolecule polypeptide in microorganism and the animal and plant body, usually by normally being formed by more than 30 amino-acid residues, molecular weight is that the bactericidal mechanism of 2-7kDa. antibacterial peptide is that it can destroy the cytolemma of bacterium, cause entocyte to overflow, cause that finally bacterium is dead.Antibacterial peptide has has a broad antifungal spectrum and be difficult for to produce the unrivaled advantages of traditional antibiotic medicine such as resistance, thermostability are strong, thereby more and more is subject to people's attention.
Antibacterial peptide is to find in plant at first.In recent years, increasing research is found also to have antibacterial peptide in many aquatic animals.Studies show that the antibacterial peptide of aquatic animal not only has anti-microbial activity, and also have antiviral and anti-tumor activity.Have the non-specific immunity system without the vertebra aquatic animal, fish then have specificity and non-specific immunity system concurrently.Resist in the process of pathogenic bacterial infection aquatic animal, non-specific immunity plays an important role.Because antibacterial peptide is the important component part of non-specific immunity system, in opposing pathogen infection process, play an important role, therefore, antibacterial peptide has huge application potential in aquiculture disease prevention.
Summary of the invention
The object of the invention is to provide a kind of antibacterial peptide and application thereof.
For achieving the above object, the technical solution used in the present invention is:
A kind of antibacterial peptide T1-17 is shown in the aminoacid sequence among the sequence table SEQ ID No.1.
The application of antibacterial peptide T1-17: described antibacterial peptide T1-17 has the effect of anti-bacteria.
Described antibacterial peptide T1-17 has the effect that suppresses the fish bacterium.Described antibacterial peptide T1-17 has the effect that suppresses Edwardsiella tarda or Vibrio anguillarum.
The present invention has following advantage: antibacterial peptide of the present invention has Wide High-efficient Antibacterial can the multiple fish bacterial pathogens of establishment, therefore is expected to be applied to the fish diseases control.
Embodiment
The invention will be further described below in conjunction with embodiment.Embodiment is intended to the description of giving an example to the present invention, but not limits the invention in any form.
Embodiment 1
Aminoacid sequence among the antibacterial peptide T1-17 tool sequence table SEQ ID No.1 of the present invention.
Sequence table SEQ ID No.1 is:
KCRRRKVHGPMIRIRKK
(a) sequence signature:
● length: 17
● type: aminoacid sequence
● chain: strand
● topological framework: linearity
(b) molecule type: protein
(c) suppose: no
(d) antisense: no
(e) initial source: synthetic
The synthetic antibacterial peptide T1-17 of present embodiment is according to BOC method solid-phase polypeptide synthesis method, finally obtains purity greater than 90% antibacterial peptide T1-17.When synthetic a Boc-amino acid derivative is covalently bonded on the resin (insoluble macromolecule resin for example; be the chloromethyl macromolecule resin); remove Boc with TFA; with in the triethylamine and free N-terminal; then by DCC activation, the next amino acid of coupling, final deprotection adopts HF method or TFMSA (trifluoromethanesulfonic acid) method more.
Referring to Schnlzer M, Alewood P, Jones A, Alewood D, Kent SBH.In situ neutralization in Boc-chemistry solid phase peptide synthesis.Int J Pept Res Ther.2007; 13:31-44.In addition, the antibacterial peptide of present embodiment utilizes glass reaction post (machine models: p3050) synthetic by Shanghai biotechnology Services Co., Ltd (Shanghai, China).
Embodiment 2
The application of antibacterial peptide T1-17
1) Edwardsiella tarda and Vibrio anguillarum preparation.In the LB substratum, cultivate Edwardsiella tarda and Vibrio anguillarum respectively to OD
600Be 0.5, centrifugal (5000g, 4 ℃) 10min then.Collect thalline, with its be suspended among the PBS to final concentration be 1x10
6Cfu/ml.
Described PBS moiety is by weight percentage: 0.8% NaCl, 0.02% KCl, 0.358%Na
2HPO
4.12H
2O, 0.024% NaH
2PO
4
2) bactericidal effect of antibacterial peptide T1-17.With 10ul above-mentioned steps 1) bacterium liquid mix with 30nMT1-17 or PBS (contrast), 25 ℃ the insulation 3-5h.Subsequently mixed solution is coated the LB solid plate, cultivated 32-48h for 28 ℃.Enumeration is found, compares with PBS, and T1-17 processes that to cause the Edwardsiella tarda number to lower about 90%, and Vibrio anguillarum then can not survive fully, and namely about 90% Edwardsiella tarda and 100% Vibrio anguillarum are killed by T1-17.Therefore, T1-17 has efficient germicidal action, is expected to be applied to the control of fish bacterial disease.
Claims (4)
1. antibacterial peptide T1-17 is characterized in that: antibacterial peptide T1-17 is shown in the aminoacid sequence among the sequence table SEQ ID No.1.
2. the application of an antibacterial peptide T1-17 claimed in claim 1 is characterized in that: the purposes of described antibacterial peptide T1-17 in preparation anti-bacteria medicine.
3. by the application of antibacterial peptide T1-17 claimed in claim 2, it is characterized in that: the purposes of described antibacterial peptide T1-17 in preparation inhibition fish bacterium medicine.
4. by the application of claim 2 or 3 described antibacterial peptide T1-17, it is characterized in that: the purposes of described antibacterial peptide T1-17 in preparation inhibition Edwardsiella tarda or Vibrio anguillarum medicine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010552417 CN102140130B (en) | 2010-11-12 | 2010-11-12 | Antibacterial peptide and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010552417 CN102140130B (en) | 2010-11-12 | 2010-11-12 | Antibacterial peptide and use thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102140130A CN102140130A (en) | 2011-08-03 |
| CN102140130B true CN102140130B (en) | 2013-03-27 |
Family
ID=44407942
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010552417 Expired - Fee Related CN102140130B (en) | 2010-11-12 | 2010-11-12 | Antibacterial peptide and use thereof |
Country Status (1)
| Country | Link |
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| CN (1) | CN102140130B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102351949B (en) * | 2011-10-25 | 2013-07-31 | 中国农业科学院植物保护研究所 | Xenorhabdus antibacterial peptide and application thereof in inhibiting growth of pathogenic fungi and bacteria |
| CN102786581A (en) * | 2012-01-09 | 2012-11-21 | 中国农业科学院植物保护研究所 | Brevibacillus laterosporus antibacterial peptide and application of Brevibacillus laterosporus antibacterial peptide in pathogenic fungus inhibition and bacterium growth |
| CN106749534B (en) * | 2016-12-07 | 2020-04-14 | 青岛农业大学 | Sebastes schlegeli antibacterial peptide BS20 and application thereof |
| CN107501411A (en) * | 2017-07-03 | 2017-12-22 | 汕头大学 | A kind of hemocyanin in shrimp Litopenaeus vannamei antibacterial peptide and its application |
| CN112961233B (en) * | 2021-03-01 | 2022-09-13 | 中国科学院海洋研究所 | Application of deep sea chitin peptide |
| CN113880933B (en) * | 2021-11-12 | 2023-10-20 | 青岛农业大学 | Antibacterial peptide SsNKL27 and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1763092A (en) * | 2005-11-03 | 2006-04-26 | 中山大学 | Antibiotic peptide and its coding sequence and uses |
| CN1821271A (en) * | 2005-12-29 | 2006-08-23 | 中山大学 | Antibiotic peptide and its code sequence and use |
| CN101191129A (en) * | 2006-11-28 | 2008-06-04 | 武汉大学 | Gene engineering domestic silkworm antibiotic peptide and its preparation method and application |
| CN101314772A (en) * | 2007-05-28 | 2008-12-03 | 上海汉德食品有限公司 | Recombination expression and application of shrimp antimicrobial peptide genes in probiotic bacteria |
-
2010
- 2010-11-12 CN CN 201010552417 patent/CN102140130B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1763092A (en) * | 2005-11-03 | 2006-04-26 | 中山大学 | Antibiotic peptide and its coding sequence and uses |
| CN1821271A (en) * | 2005-12-29 | 2006-08-23 | 中山大学 | Antibiotic peptide and its code sequence and use |
| CN101191129A (en) * | 2006-11-28 | 2008-06-04 | 武汉大学 | Gene engineering domestic silkworm antibiotic peptide and its preparation method and application |
| CN101314772A (en) * | 2007-05-28 | 2008-12-03 | 上海汉德食品有限公司 | Recombination expression and application of shrimp antimicrobial peptide genes in probiotic bacteria |
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|---|---|
| CN102140130A (en) | 2011-08-03 |
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